Plant extract composition with whitening and brightening effects and application thereof

Abstract

The invention discloses a plant extract composition with whitening and brightening effects and application of the plant extract composition. The plant extraction composition with the whitening and brightening effects is mainly prepared from a rhizoma bletillae (BLETILLA STRIATA) root extract and a poria cocos (PORIA COCOS) sclerotium extract. According to the invention, plant compositions of rhizoma bletillae and poria cocos are carefully selected, and the plant extract composition with whitening and brightening effects is obtained by means of modern biological fermentation, ultrahigh pressure extraction and other technical means. Tests prove that the composition can effectively inhibit melanogenesis, can also inhibit non-enzymatic glycosylation reaction, and has good whitening and brightening effects when being applied to cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a plant extract composition with whitening and brightening effects and application thereof. Background Art

[0002] "A white complexion hides all ugliness" has always been the aesthetic philosophy of oriental women, and "skin as white as snow and as smooth as fat" has always been a skin condition that has been admired and pursued. However, with the accelerated pace of life and work and the intensification of environmental pollution, people are facing more and more skin problems, and dull skin color and pigmentation have become common problems. There are still some unclear points about the mechanism of the above-mentioned dull skin color and pigmentation problems. Traditional research believes that melanin deposition is the main cause of the above problems. However, with the development of life sciences, people have found that non-enzymatic glycosylation reactions in the skin are also one of the main causes of the above problems.

[0003] About melanin deposition. Skin melanin is a protein, and its metabolic pathway is as follows: in melanocytes, tyrosine is gradually converted into true melanin through intermediates such as dopa, dopaquinone, dopachrome, and dihydroxyindole under the action of enzymes such as tyrosinase, and then transferred to the epidermal basal cells through the top of the melanocyte dendrites, and enters the stratum corneum with the metabolism of the cells, and finally falls off with the keratinized cells. However, when melanin grows too fast and is unevenly distributed, it will cause local skin to be too dark and pigmented.

[0004] About non-enzymatic glycosylation reaction. The non-enzymatic glycosylation theory believes that reducing sugars and free amino groups of proteins in the body undergo a complex process to eventually generate brown or even black macromolecules, namely glycosylation end products (AGEs). Specifically, it starts with the nucleophilic addition reaction of amino free radicals and reducing carbonyl compounds, followed by the generation of Schiff bases; molecular rearrangement is carried out on the basis of this Schiff base to form stable Amadori products; glycosylated proteins undergo further reactions to form dicarbonyl intermediates (such as: 3-deoxyglucose), and finally generate AGEs.

[0005] Therefore, products that inhibit both melanin production and non-enzymatic glycation reactions will achieve better whitening effects.

[0006] After extensive research in the field of cosmetics, the inventors of the present invention discovered that a plant extract composition was obtained by using Bletilla striata as a substrate and solid-state fermentation with Poria cocos mycelium, which can effectively inhibit melanin production and non-enzymatic glycosylation reactions. The plant extract composition has good whitening and brightening effects when used in cosmetics, thus completing the present invention. Summary of the invention

[0007] In view of this, the object of the present invention is to provide a plant extract composition with whitening and brightening effects and its application in cosmetics.

[0008] In order to achieve the above-mentioned object of the invention, the specific technical scheme of the present invention is as follows: A plant extract composition with whitening and brightening effects, characterized in that it mainly contains Bletilla striata root extract and Poria cocos sclerotium extract.

[0009] The Bletilla striata described in the present invention is a hemostatic drug with the effects of astringing and stopping bleeding, reducing swelling and promoting tissue regeneration. Bletilla striata is also known as the "whitening fairy" among herbs. The Treatise on Materia Medica says that Bletilla striata "treats facial sores and makes the skin smooth"; the Compendium of Materia Medica also says that it "washes away dark spots on the face and removes freckles."

[0010] The Poria cocos described in the present invention is a fungus that can be used as both food and medicine, and has multiple biological activities, such as strengthening the spleen, reducing lipids, anti-tumor, immune regulation and anti-oxidation, etc. Poria cocos has a strong ability to degrade natural macromolecules such as cellulose and lignin, and its mycelium can also be used for liquid or solid fermentation.

[0011] The embodiment of the present invention provides a method for preparing the plant extract composition having whitening and brightening effects, comprising the following steps: (1) Weigh the following raw materials in parts by weight: 1-10 parts of Bletilla striata root, dry in the shade, crush and sieve, sterilize, and obtain a mixed powder; (2) Weigh 1-10 parts by weight of Poria cocos, add 50-500 parts by weight of PDA culture medium, and perform culture activation; then add the mixed powder, mix well, and perform solid-state fermentation to obtain a fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:10-15, stirring and soaking at a temperature of 45-55 degrees for 1-2 hours, performing ultrahigh pressure assisted extraction at 50-100 MPa for 1-3 minutes, and centrifuging to obtain the supernatant; (4) The supernatant is allowed to stand, and then frozen and filtered to obtain the plant extract composition.

[0012] In some embodiments, 1-10 portions of Bletilla striata flowers may be added in step (1).

[0013] In some embodiments, the sieving in step (1) is sieving through a 20-50 mesh sieve; and the sterilization is sealing and sterilizing at 121° C. for 120 min.

[0014] In one embodiment, the weight proportions of the raw materials are: 8 parts of Bletilla striata root, 3 parts of Bletilla striata flower, and 5 parts of Poria cocos.

[0015] The PDA medium described in step (2) of the present invention is the abbreviation of potato dextrose agar medium. PDA medium can be homemade or commercially available. The homemade process is as follows: 200 g of potatoes are cut into small pieces, boiled for 30 minutes, filtered through gauze, 20 g of glucose and 20 g of agar are added, the volume is fixed to 1000 mL, and sterilized at 121°C for 20 minutes.

[0016] In some embodiments, the culture activation in step (2) is to culture at a temperature of 20-25°C and a humidity of 60-63% for 12-24 hours; the solid-state fermentation is to culture at a temperature of 20-25°C and a humidity of 60-63% for 24-48 hours, and the fermentation is stopped after the Poria mycelium fills the entire culture chamber.

[0017] The ultra-high pressure assisted extraction used in step (3) of the present invention rapidly increases the pressure from normal pressure to hundreds of MPa within a few minutes, forming a huge pressure difference between the inside and outside of the solid cells. The extraction solvent rapidly penetrates into the vascular bundles and cells inside the plant under the push of the ultra-high pressure, causing structural changes such as loosening, holes, and ruptures in the cell structure, and the active substances in the cells are fully contacted with the solvent and dissolved.

[0018] In some of the embodiments, the mass ratio of butanediol to water in the butanediol aqueous solution in step (3) is 1:3-5; and the centrifugation refers to centrifugation at a speed of 5000-10000 rpm for 30-60 minutes.

[0019] In some embodiments, the freeze filtration in step (4) refers to standing at a temperature of 1-5 degrees for 12-24 hours and then filtering with a 0.1-1 μm filter membrane. Freeze filtration allows fine precipitates suspended in the liquid to be precipitated in advance and filtered clean, thereby preventing precipitation from occurring during long-term storage of the product.

[0020] The plant extract composition with whitening and brightening effects prepared in the embodiment of the present invention can be used as an active ingredient in a cosmetic formula, and its addition amount can be 1-10wt%. The cosmetic can be in the form of cream, lotion, facial mask, essence, facial cleanser, etc.

[0021] The embodiment of the present invention also provides a plant-based whitening cream, which is composed of the following raw materials in percentage by weight: 2% butylene glycol, 0.1% xanthan gum, 0.35% ammonium acryloyldimethyltaurate / VP copolymer, 0.65% acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 0.3% allantoin, 0.1% sodium hyaluronate, 0.5% p-hydroxyacetophenone, 1% polysorbate-60, 1% cetearyl alcohol, 4% isononyl isononanoate, 2% polydimethylsiloxane, 0.5% tocopheryl acetate, 0.1% ceramide NP, 0.2% stearyl glycyrrhetinate, 0.55% triethanolamine, 0.2% polyacrylamide, 0.2% C13-14 isoparaffin, 0.2% laureth-7, 1% trehalose, 0.8% 1,2-hexanediol, 0.2% phenoxyethanol, 1-10% of the above-mentioned plant-based composition with whitening and brightening effects, and the balance water.

[0022] The technical solution provided by the embodiment of the present invention has the following beneficial effects: (1) The present invention provides a method for preparing a plant extract composition with whitening and brightening effects. The preparation method requires, on the one hand, extracting the active ingredients of Poria cocos itself, and on the other hand, ingeniously utilizing Poria cocos mycelium for solid-state fermentation to bioconvert natural macromolecules such as cellulose and lignin in raw materials such as Bletilla striata into small molecules, thereby increasing the active ingredient content of the composition. The preparation method of the present invention also uses an ultrahigh pressure assisted extraction step, which is conducive to further dissolution and extraction of the active ingredients.

[0023] (2) The present invention selects Bletilla striata and Poria cocos as a plant formula, and obtains a plant extract composition with whitening and brightening effects through modern biological fermentation, ultra-high pressure extraction and other technical means. Experiments have confirmed that it can effectively inhibit the formation of melanin and non-enzymatic glycosylation reactions, and its application in cosmetics has a good whitening and brightening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0025] Figure 1 The flavonoid content of each test group is shown; Figure 2 Shows the AGEs inhibition rate of each test group; Figure 3 The tyrosinase inhibition rate of each test group is shown. DETAILED DESCRIPTION

[0026] The embodiments in the specification are only used to illustrate the present invention and do not limit the scope of protection of the present invention. The scope of protection of the present invention is only limited by the claims, and any omission, replacement or modification made on the basis of the embodiments disclosed in the present invention shall fall within the scope of protection of the present invention.

[0027] The content of the present invention can be more easily understood by selecting the following detailed description of the preferred implementation method of the present invention and the embodiments included. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs. When there is a conflict, the definition in this specification shall prevail.

[0028] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0029] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0030] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously intended to be in the singular form.

[0031] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention. Example 1

[0032] A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 1 part of Bletilla striata root, dry in the shade, crush through a 50-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 10 parts by weight of Poria cocos, add 50 parts by weight of PDA culture medium, and incubate at 25°C and 60% humidity for 24 hours for activation; then add the mixed powder, mix well, and incubate at 20°C and 63% humidity for 24 hours. When the Poria cocos mycelium fills the entire culture chamber, stop fermentation to obtain a fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:15, wherein the mass ratio of butanediol to water in the butanediol aqueous solution is 1:3; stirring and soaking at a temperature of 55 degrees for 1 hour, performing ultrahigh pressure assisted extraction at 100 MPa for 1 minute, centrifuging at a speed of 10,000 rpm for 30 minutes, and taking the supernatant; (4) The supernatant is allowed to stand at 5 degrees for 12 hours, and then filtered through a 1 μm filter membrane to obtain a plant extract composition. Example 2

[0033] A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 10 parts of Bletilla striata root, dry in the shade, crush through a 20-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 1 part by mass of Poria cocos, add 500 parts by mass of PDA culture medium, and incubate for 12 hours at a temperature of 20°C and a humidity of 63% for activation; then add the mixed powder, mix well, and incubate for 48 hours at a temperature of 25°C and a humidity of 60%. When the Poria cocos mycelium fills the entire culture chamber, the fermentation is stopped to obtain the fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:10, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:5; stirring and soaking at a temperature of 45 degrees for 2 hours, performing ultrahigh pressure assisted extraction at 50 MPa for 3 minutes, and centrifuging at a speed of 5000 rpm for 60 minutes to obtain the supernatant; (4) The supernatant is allowed to stand at 1°C for 24 hours and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition. Example 3

[0034] A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 8 parts of Bletilla striata root, dry in the shade, crush through a 40-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 5 parts by weight of Poria cocos, add 300 parts by weight of PDA culture medium, and incubate for 18 hours at a temperature of 22°C and a humidity of 62% for activation; then add the mixed powder, mix well, and incubate for 36 hours at a temperature of 22°C and a humidity of 61%. When the Poria cocos hyphae fill the entire culture chamber, stop the fermentation and obtain the fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:12, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (4) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0035] Test-flavonoid content and anti-glycation test This test uses the plant extract composition with whitening and brightening effects prepared in Examples 1-3 as the research object, and evaluates the anti-glycation ability of the plant extract composition prepared in the examples of the present invention by measuring the flavonoid content in the plant extract composition and conducting an anti-glycation test.

[0036] In order to investigate the interaction between the root of Bletilla striata and Poria cocos in the plant extract composition of the present invention, and the difference between the preparation process of the present invention and the conventional process, comparative examples 1-3 are specially set.

[0037] Comparative Example 1 A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 8 parts of Bletilla striata root and 5 parts of Poria cocos, dry in the shade, and grind through a 40-mesh sieve to obtain a mixed powder; (2) adding a butanediol aqueous solution to the mixed powder, with the solid-liquid ratio controlled at 1:12, wherein the mass ratio of butanediol to water in the butanediol aqueous solution is 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (3) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0038] Comparative Example 2 A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh 13 parts by weight of Poria cocos, add 300 parts by weight of PDA culture medium, and incubate for 18 hours at a temperature of 22°C and a humidity of 62% for activation; then add the mixed powder, mix well, and incubate for 36 hours at a temperature of 22°C and a humidity of 61%. When the Poria cocos hyphae fill the entire culture chamber, the fermentation is stopped to obtain a fermentation product; (2) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:12, wherein the mass ratio of butanediol to water in the butanediol aqueous solution is 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (3) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0039] Comparative Example 3 A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 13 parts of Bletilla striata root, dry in the shade, crush through a 40-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) adding a butanediol aqueous solution to the mixed powder, with the solid-liquid ratio controlled at 1:12, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (3) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0040] A. Determination of total flavonoids content The extracellular flavonoids content was determined by the aluminum chloride method. Accurately pipette 1.0 mL of the test solution into a 10 mL volumetric flask, add 2.0 mL of 0.1 mol / L aluminum chloride solution and 3.0 mL of 1.0 mol / L potassium acetate solution, respectively, dilute to the scale with 70% methanol solution, shake well, and place at room temperature for 30 minutes. Centrifuge at 4000 r / min for 10 minutes, and measure the absorbance at a wavelength of 420 nm. Draw a standard curve with rutin as the standard, and calculate the extracellular flavonoids content according to the standard curve equation. The specific test results are attached. Figure 1 .

[0041] From the attached Figure 1 It can be seen that the total flavonoid content in the plant extract composition with whitening and brightening effect prepared in Examples 1-3 of the present invention is 0.32-0.43 g / L, which is higher than that in Comparative Example 1 using conventional process and Comparative Examples 2-3 using Bletilla striata or Poria cocos.

[0042] B. Anti-glycation test This test refers to the method described in [1] Cao Yimiao, Gao Hongqi, Gao Cong, et al. Study on the active ingredients and skin care effects of camellia at high and low altitudes [J]. Daily Chemicals Science, 2024, 47(01): 29-32. Anti-glycation activity test-advanced glycation end products (AGEs) inhibition rate: bovine serum albumin and glucose were dissolved in 0.05 mol / L phosphate buffer to prepare 8 mg / mL bovine serum albumin and 0.2 mol / L glucose phosphate buffer. First, 50 µL of the sample diluted to a certain concentration was added to the sample tube, 50 µL of phosphate buffer was added to the modeling group, and then 300 µL of phosphate buffer was added to each. After incubation at 60°C for 40 hours, 200 µL of each sample was added to a black 96-well plate, and the fluorescence value at the excitation wavelength of 370 nm / emission wavelength of 440 nm was detected by an ELISA reader, and 0.04% aminoguanidine was used as a positive control. AGEs inhibition rate = (1-A sample / A modeling group) × 100%. See the attached for specific test results Figure 2 .

[0043] By the attached Figure 2 It can be seen that the plant extract compositions with whitening and brightening effects prepared in Examples 1-3 of the present invention have comparable effects to the positive control of 0.04% aminoguanidine, indicating that the plant extract compositions prepared in the present invention have good anti-glycation activity.

[0044] Test Dityrosinase inhibition assay This test uses the preferred embodiment 3 as the test object, and evaluates the whitening ability of the plant extract composition prepared in the embodiment of the present invention by measuring the tyrosinase inhibition rate in the plant extract composition.

[0045] In order to investigate the interaction between the Bletilla striata flower and the Bletilla striata root in the plant extract composition of the present invention, Example 4 and Comparative Examples 4-5 are specially set. Example 4

[0046] A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 8 parts of Bletilla striata root and 3 parts of Bletilla striata flower, dry in the shade, grind through a 40-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 5 parts by weight of Poria cocos, add 300 parts by weight of PDA culture medium, and incubate for 18 hours at a temperature of 22°C and a humidity of 62% for activation; then add the mixed powder, mix well, and incubate for 36 hours at a temperature of 22°C and a humidity of 61%. When the Poria cocos hyphae fill the entire culture chamber, stop the fermentation and obtain the fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:12, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (4) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0047] Comparative Example 4 A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials in parts by weight: 11 parts of Bletilla striata root, dry in the shade, crush through a 40-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 5 parts by weight of Poria cocos, add 300 parts by weight of PDA culture medium, and incubate for 18 hours at a temperature of 22°C and a humidity of 62% for activation; then add the mixed powder, mix well, and incubate for 36 hours at a temperature of 22°C and a humidity of 61%. When the Poria cocos hyphae fill the entire culture chamber, stop the fermentation and obtain the fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:12, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (4) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0048] Comparative Example 5 A plant extract composition with whitening and brightening effects is prepared by the following steps: (1) Weigh the following raw materials by weight: 11 parts of Bletilla striata flower, dry in the shade, crush through a 40-mesh sieve, seal and sterilize at 121°C for 120 min to obtain a mixed powder; (2) Weigh 5 parts by weight of Poria cocos, add 300 parts by weight of PDA culture medium, and incubate for 18 hours at a temperature of 22°C and a humidity of 62% for activation; then add the mixed powder, mix well, and incubate for 36 hours at a temperature of 22°C and a humidity of 61%. When the Poria cocos hyphae fill the entire culture chamber, stop the fermentation and obtain the fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:12, and the mass ratio of butanediol to water in the butanediol aqueous solution being 1:4; stirring and soaking at a temperature of 50 degrees for 1.5 hours, performing ultrahigh pressure assisted extraction at 80 MPa for 2 minutes, centrifuging at a speed of 8000 rpm for 50 minutes, and taking the supernatant; (4) The supernatant is allowed to stand at 3 degrees for 18 hours, and then filtered through a 0.1 μm filter membrane to obtain a plant extract composition.

[0049] C. Tyrosinase inhibition test Melanin is a type of high-molecular compound that causes skin darkening. It usually starts with L-tyrosine. After being catalyzed by tyrosinase, L-dopa and dopaquinone are successively generated, and after multiple steps of reaction, melanin is finally synthesized. Tyrosinase continuously catalyzes two rate-limiting reactions and is a key enzyme in the melanin synthesis process. Therefore, the tyrosinase activity inhibition rate is an important indicator for the evaluation of the whitening effect of cosmetics.

[0050] This test refers to the method described in the literature "Lin Shengjie, Yang Dengliang, Duan Jun, et al. Study on the synergistic inhibitory effect of Dendrobium officinale, Ginseng and Pueraria extracts on tyrosinase diphenolase activity [J]. Contemporary Chemical Research, 2024, (19): 42-44." Take a test tube and add 0.4mL of 1.0mg / mL L-dopa solution as substrate, add an appropriate amount of phosphate buffer, and bathe at 30℃ for 10min, then add 0.2mL of 250U / mL tyrosinase solution. Mix and read the absorbance (A475) at a wavelength of 475nm when the time is 1min. Calculate the enzyme activity inhibition rate according to the following formula. Inhibition rate = [1-(Ac-Ad) / (Aa-Ab)]×100%. Among them, the mass concentration of the test substance refers to the final mass concentration of the additive in the test solution. Each absorbance value in the formula represents the following different situations: Aa is the situation of containing only substrate without adding test composition additives; Ab is the situation of not adding substrate and test substance; Ac is the situation of containing substrate and test substance; Ad is the situation of not adding substrate but containing test substance. Specific test results are shown in the attached Figure 3 .

[0051] By the attached Figure 3 It can be seen that the plant extract composition with whitening and brightening effects prepared in Example 4 of the present invention has a good inhibitory effect on tyrosinase.

[0052] Test 3 Volunteer Efficacy Evaluation In order to further verify the whitening effect of the plant extract composition prepared in the embodiment of the present invention, Example 4 was applied to the cosmetic formula, and the whitening effect of the plant extract composition prepared in the embodiment of the present invention was confirmed through human body efficacy evaluation. Example 5

[0053] A plant extract whitening cream is composed of the following raw materials in percentage by weight: 2% butylene glycol, 0.1% xanthan gum, 0.35% ammonium acryloyldimethyltaurate / VP copolymer, 0.65% acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer, 0.3% allantoin, 0.1% sodium hyaluronate, 0.5% p-hydroxyacetophenone, 1% polysorbate-60, 1% cetearyl alcohol, 4% isononyl isononanoate, 2% polydimethylsiloxane, 0.5% tocopheryl acetate, 0.1% ceramide NP, 0.2% stearyl glycyrrhetinate, 0.55% triethanolamine, 0.2% polyacrylamide, 0.2% C13-14 isoparaffin, 0.2% laureth-7, 1% trehalose, 0.8% 1,2-hexanediol, 0.2% phenoxyethanol, 10% of the plant extract composition with whitening and brightening effects prepared in Example 4, and the balance water.

[0054] D. Human efficacy evaluation test 30 volunteers aged 25-50 with dark complexion and pigmentation problems were recruited to participate voluntarily and sign informed consent forms, and they have not received other clinical efficacy tests recently. After cleansing their faces, the volunteers used the plant extract whitening cream prepared in Example 5 of the present invention, applied an appropriate amount on their faces, and gently massaged in circles for absorption. Once in the morning and evening, after one month of continuous use, the volunteers were revisited and the self-perception evaluation data were statistically analyzed. It was found that 18 of the volunteers reported that their skin became delicate, rosy and shiny, and the dark complexion and pigmentation problems were significantly improved. 8 volunteers had dark complexion and pigmentation problems that improved to a certain extent. The total effective rate exceeded 80%, and all volunteers reported that there was no irritation or allergy. It can be seen that the plant extract composition prepared in the embodiment of the present invention can play a good whitening effect when applied to the human body.

[0055] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0056] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A plant extract composition with whitening and brightening effects, characterized in that: It mainly contains Bletilla striata root extract and Poria cocos sclerotium extract.

2. The plant extract composition with whitening and brightening effects according to claim 1, characterized in that: The preparation method thereof comprises the following steps: (1) Weigh the following raw materials in parts by weight: 1-10 parts of Bletilla striata root, dry in the shade, crush and sieve, sterilize, and obtain a mixed powder; (2) Weigh 1-10 parts by weight of Poria cocos, add 50-500 parts by weight of PDA culture medium, and perform culture activation; then add the mixed powder, mix well, and perform solid-state fermentation to obtain a fermentation product; (3) adding a butanediol aqueous solution to the fermentation product, with the solid-liquid ratio controlled at 1:10-15, stirring and soaking at a temperature of 45-55 degrees for 1-2 hours, performing ultrahigh pressure assisted extraction at 50-100 MPa for 1-3 minutes, and centrifuging to obtain the supernatant; (4) The supernatant is allowed to stand, and then filtered by freezing to obtain the plant extract composition.

3. The plant extract composition with whitening and brightening effects according to claim 2, characterized in that: In step (1), 1-10 portions of Bletilla striata flowers may also be added.

4. The plant extract composition with whitening and brightening effects according to claim 2, characterized in that: The sieving in step (1) is sieving through a 20-50 mesh sieve; the sterilization is sealing and sterilizing at 121° C. for 120 min.

5. The plant extract composition with whitening and brightening effects according to claim 3, characterized in that: The weight portions of the raw materials are: 8 parts of Bletilla striata root, 3 parts of Bletilla striata flower, and 5 parts of Poria cocos.

6. The plant extract composition with whitening and brightening effects according to claim 2, characterized in that: The culture activation described in step (2) is to culture at a temperature of 20-25°C and a humidity of 60-63% for 12-24 hours; the solid-state fermentation is to culture at a temperature of 20-25°C and a humidity of 60-63% for 24-48 hours, and the fermentation is stopped when the Poria mycelium fills the entire culture chamber.

7. The plant extract composition with whitening and brightening effects according to claim 2, characterized in that: The mass ratio of butanediol to water in the butanediol aqueous solution in step (3) is 1:3-5; the centrifugation refers to centrifugation at a speed of 5000-10000 rpm for 30-60 minutes.

8. The plant extract composition with whitening and brightening effects according to claim 2, characterized in that: The freeze filtration described in step (4) refers to standing at a temperature of 1-5 degrees for 12-24 hours and then filtering with a 0.1-1 μm filter membrane.

9. Use of the plant extract composition with whitening and brightening effects according to any one of claims 1 to 8 in the preparation of cosmetics.

10. A plant extract whitening cream, characterized in that: The invention is composed of the following raw materials in percentage by weight: 2% butylene glycol, 0.1% xanthan gum, 0.35% ammonium acryloyldimethyltaurate / VP copolymer, 0.65% acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.3% allantoin, 0.1% sodium hyaluronate, 0.5% p-hydroxyacetophenone, 1% polysorbate-60, 1% cetearyl alcohol, 4% isononyl isononanoate, 2% polydimethylsiloxane, 0.5% tocopheryl acetate, 0.1% ceramide NP, 0.2% stearyl glycyrrhetinate, 0.55% triethanolamine, 0.2% polyacrylamide, 0.2% C13-14 isoparaffin, 0.2% laureth-7, 1% trehalose, 0.8% 1,2-hexanediol, 0.2% phenoxyethanol, 1-10% of the plant extract composition with whitening and brightening effects according to any one of claims 1 to 8, and the balance water.

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