Novel extraction process for natural Chinese medicine

Abstract

The invention discloses a novel extraction process of natural Chinese medicine, relates to the field of Chinese medicine fermentation technology, and comprises the following steps: S1, preparation of Chinese medicinal materials; S2, fine treatment; S3, extraction of Chinese medicine stock solution; S4, preparation of fermentation liquid: firstly take glucose 18-20g, yeast extract 3-5g, inorganic salt 0.5-1g, coenzyme 0.5-1g Chinese medicine stock solution 10-12g and surplus pure water in proportion, pour them into a fermentation tank in turn and mix them into a culture liquid, then culture the strain in the culture liquid for 48-36h, and stir and mix once every 12h to finally obtain the fermentation liquid; S5, fermentation. The present invention extracts the stock solution of Chinese medicine raw materials, and uses the Chinese medicine stock solution, culture liquid and strains to mix and ferment in a fermentation tank to form a novel Chinese medicine fermentation processing preparation of a full-component fermentation liquid containing Chinese medicine active ingredients, thalline and its metabolites, so that the quality stability of the fermented Chinese medicine can be greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicine fermentation, in particular to a novel process for extracting natural Chinese medicine. Background Art

[0002] Traditional Chinese medicine fermentation technology is a technology that processes traditional Chinese medicines into traditional Chinese medicine products with better medicinal effects through fermentation. In fermentation technology, microorganisms are the main fermentation agents, which can convert the active ingredients in traditional Chinese medicines into a form that is more easily absorbed by the human body through fermentation, thereby improving the efficacy of traditional Chinese medicine products. At the same time, it can also improve the aroma, taste and appearance quality of traditional Chinese medicines.

[0003] However, the current Chinese medicine fermentation technology mainly adopts multi-strain mixed natural fermentation. The types and quantities of bacteria participating in the fermentation fluctuate to a certain extent. At the same time, the fermentation and processing of traditional Chinese medicine adopts solid fermentation. The entire fermentation and processing process is controlled by subjective experience. Therefore, the quality stability of traditional Chinese medicine after fermentation and processing is difficult to guarantee. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a novel process for extracting natural Chinese medicine, which solves the problems raised in the above-mentioned background technology.

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: a new process for extracting natural Chinese medicine, comprising the following steps:

[0006] S1. Preparation of Chinese medicinal materials: fresh Chinese medicinal materials are selected as fermentation raw materials, and the fermentation raw materials are pre-treated in sequence by washing, sun drying, and impurity removal;

[0007] S2, fine treatment: first dehydrate the pretreated fermentation raw materials, and then use a pulverizer to pulverize part of the fermentation raw materials;

[0008] S3. Extraction of Chinese medicine stock solution: directly placing the raw material particles into a supercritical extraction device to extract the stock solution or first mixing the raw material particles with purified water and boiling them in a medicine decoction machine, and then using an extraction concentrator to extract the stock solution from the boiled medicine soup;

[0009] S4, preparation of fermentation liquid: firstly, according to the proportion, 18-20g of glucose, 3-5g of yeast extract, 0.5-1g of inorganic salt, 0.5-1g of coenzyme, 10-12g of Chinese medicine stock solution and the remaining amount of purified water are poured into a fermentation tank in sequence and mixed to prepare a culture liquid, and then the strain is cultured in the culture liquid for 48-36h, and stirred and mixed once every 12h to finally obtain a fermentation liquid;

[0010] S5. Fermentation: put the remaining solid Chinese medicine raw materials to be fermented into a fermentation tank, mix and stir evenly, and ferment in a sealed manner for 24-48 hours. During the fermentation process, maintain the oxygen content in the tank at 8-20%. First, ferment at 30-33°C for 12-24 hours, then add fermentation liquid, and then ferment again at 33-37°C for 12-24 hours to obtain the desired fermented medicine.

[0011] Furthermore, in step S2, the moisture content of the fermentation raw material after dehydration is 15-20%.

[0012] Furthermore, in step S3, the fermentation raw material used for direct extraction by the supercritical extraction equipment has a particle size of 200 mesh or more after crushing.

[0013] Furthermore, the particle size of the fermented raw material after crushing for boiling in the medicine decocting machine in step S3 is above 10 meshes.

[0014] Furthermore, the bacteria cultured in step S4 are one or more of Bacillus, yeast, and lactic acid bacteria.

[0015] Furthermore, when culturing the strains in step S4, the temperature in the tank is maintained at 35-37°C.

[0016] The present invention provides a novel process for extracting natural Chinese medicine, which has the following beneficial effects:

[0017] The invention extracts the stock solution of Chinese medicine raw materials, and uses the Chinese medicine stock solution, culture solution and bacterial strains for mixed fermentation in a fermentation tank to form a novel Chinese medicine fermentation processing preparation containing a full-component fermentation solution of Chinese medicine active ingredients, bacteria and their metabolites. Not only can the types and quantity of bacterial strains involved in the fermentation be better controlled, but the temperature, humidity, pH, ventilation and the like can also be better dynamically controlled, so that the quality stability of the fermented Chinese medicine can be greatly improved. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with specific embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0019] Embodiment 1, the present invention provides a technical solution: a new process for extracting natural Chinese medicine, comprising the following steps:

[0020] S1. Preparation of Chinese medicinal materials: fresh Chinese medicinal materials are selected as fermentation raw materials, and the fermentation raw materials are pre-treated in sequence by washing, sun drying, and impurity removal;

[0021] S2, fine treatment: first dehydrate the fermentation raw materials after pretreatment, the moisture content of the fermentation raw materials after dehydration is 15%, and then use a pulverizer to crush part of the fermentation raw materials, and the particle size of the fermentation raw materials after crushing is more than 200 meshes;

[0022] S3. Extraction of Chinese medicine stock solution: directly placing the raw material particles into the supercritical extraction equipment to extract the stock solution;

[0023] S4, preparation of fermentation liquid: first, according to the proportion, 18g of glucose, 3g of yeast extract, 0.5g of inorganic salt, 0.5g of coenzyme, 10g of Chinese medicine stock solution and the remaining amount of pure water are poured into a fermentation tank in sequence and mixed to prepare a culture liquid, and then the strains are cultured in the culture liquid for 48h, and the strains are one or more of Bacillus, yeast, and lactic acid bacteria. The temperature in the tank is maintained at 35°C, and the mixture is stirred every 12h to finally obtain the fermentation liquid;

[0024] S5. Fermentation: put the remaining solid Chinese medicine raw materials to be fermented into a fermentation tank, mix and stir evenly, and ferment in a sealed manner for 24 hours. During the fermentation process, maintain the oxygen content in the tank at 8%. First, ferment at 30°C for 12 hours, then add the fermentation liquid, and then ferment for a second time at 33°C for 12 hours to obtain the desired fermented medicine.

[0025] The process in this example utilizes the mixed fermentation of Chinese medicine stock solution, culture medium and bacterial strain in a fermentation tank to form a new type of Chinese medicine fermentation processing preparation containing a full-component fermentation liquid of Chinese medicine active ingredients, bacteria and their metabolites, which greatly improves the quality stability of the fermented Chinese medicine.

[0026] Embodiment 2, novel process for extracting natural Chinese medicine, comprises the following steps:

[0027] S1. Preparation of Chinese medicinal materials: fresh Chinese medicinal materials are selected as fermentation raw materials, and the fermentation raw materials are pre-treated in sequence by washing, sun drying, and impurity removal;

[0028] S2, fine treatment: first dehydrate the fermentation raw materials after pretreatment, the moisture content of the fermentation raw materials after dehydration is 20%, and then use a pulverizer to crush part of the fermentation raw materials, and the particle size of the fermentation raw materials after crushing is more than 10 meshes;

[0029] S3. Extraction of Chinese medicine stock solution: first mix the raw material particles with purified water and put them into a medicine decoction machine for boiling, and then use an extraction concentrator to extract the stock solution from the boiled medicine soup;

[0030] S4, preparation of fermentation liquid: first, according to the proportion, 20g of glucose, 5g of yeast extract, 1g of inorganic salt, 1g of coenzyme, 12g of Chinese medicine stock solution and the remaining amount of pure water are poured into a fermentation tank in sequence and mixed to prepare a culture liquid, and then the strains are cultured in the culture liquid for 36h, the strains are one or more of Bacillus, yeast, and lactic acid bacteria, the temperature in the tank is maintained at 36°C, and the mixture is stirred every 12h to finally obtain the fermentation liquid;

[0031] S5. Fermentation: put the remaining solid Chinese medicine raw materials to be fermented into a fermentation tank, mix and stir evenly, and ferment in a sealed manner for 48 hours. During the fermentation process, maintain the oxygen content in the tank at 20%. First, ferment at 33°C for 24 hours, then add fermentation liquid, and then ferment again at 37°C for 24 hours to obtain the desired fermented medicine.

[0032] The process in this example utilizes the mixed fermentation of Chinese medicine stock solution, culture medium and bacterial strain in a fermentation tank to form a new type of Chinese medicine fermentation processing preparation containing a full-component fermentation liquid of Chinese medicine active ingredients, bacteria and their metabolites, which greatly improves the quality stability of the fermented Chinese medicine.

[0033] Embodiment 3, novel process for extracting natural Chinese medicine, comprising the following steps:

[0034] S1. Preparation of Chinese medicinal materials: fresh Chinese medicinal materials are selected as fermentation raw materials, and the fermentation raw materials are pre-treated in sequence by washing, sun drying, and impurity removal;

[0035] S2, fine treatment: first dehydrate the fermentation raw materials after pretreatment, the moisture content of the fermentation raw materials after dehydration is 15%, and then use a pulverizer to crush part of the fermentation raw materials, and the particle size of the fermentation raw materials after crushing is more than 200 meshes;

[0036] S3. Extraction of Chinese medicine stock solution: directly placing the raw material particles into the supercritical extraction equipment to extract the stock solution;

[0037] S4, preparation of fermentation liquid: first, according to the proportion, 19g of glucose, 2g of yeast extract, 0.8g of inorganic salt, 0.8g of coenzyme, 11g of Chinese medicine stock solution and the remaining amount of pure water are poured into a fermentation tank in sequence and mixed to prepare a culture liquid, and then the strains are cultured in the culture liquid for 48h, and the strains are one or more of Bacillus, yeast, and lactic acid bacteria. The temperature in the tank is maintained at 37°C, and the mixture is stirred every 12h to finally obtain the fermentation liquid;

[0038] S5. Fermentation: put the remaining solid Chinese medicine raw materials to be fermented into a fermentation tank, mix and stir evenly, and ferment in a sealed manner for 36 hours. During the fermentation process, maintain the oxygen content in the tank at 15%. First, ferment at 32°C for 18 hours, then add fermentation liquid, and then ferment for a second time at 35°C for 18 hours to obtain the desired fermented medicine.

[0039] The process in this example utilizes the mixed fermentation of Chinese medicine stock solution, culture medium and bacterial strain in a fermentation tank to form a new type of Chinese medicine fermentation processing preparation containing a full-component fermentation liquid of Chinese medicine active ingredients, bacteria and their metabolites, which greatly improves the quality stability of the fermented Chinese medicine.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A new process for extracting natural Chinese medicine, characterized in that: The following steps are involved: S1. Preparation of Chinese medicinal materials: fresh Chinese medicinal materials are selected as fermentation raw materials, and the fermentation raw materials are pre-treated in sequence by washing, sun drying, and impurity removal; S2, fine treatment: first dehydrate the pretreated fermentation raw materials, and then use a pulverizer to pulverize part of the fermentation raw materials; S3. Extraction of Chinese medicine stock solution: directly placing the raw material particles into a supercritical extraction device to extract the stock solution or first mixing the raw material particles with purified water and boiling them in a medicine decoction machine, and then using an extraction concentrator to extract the stock solution from the boiled medicine soup; S4, preparation of fermentation liquid: first, according to the proportion, 18-20g of glucose, 3-5g of yeast extract, 0.5-1g of inorganic salt, 0.5-1g of coenzyme, 10-12g of Chinese medicine stock solution and the remaining purified water are poured into a fermentation tank in sequence and mixed to prepare a culture liquid, and then the strain is cultured in the culture liquid for 48-36h, and stirred and mixed once every 12h to finally obtain a fermentation liquid; S5. Fermentation: put the remaining solid Chinese medicine raw materials to be fermented into a fermentation tank, mix and stir evenly, and ferment in a sealed manner for 24-48 hours. During the fermentation process, maintain the oxygen content in the tank at 8-20%. First, ferment at 30-33°C for 12-24 hours, then add fermentation liquid, and then ferment again at 33-37°C for 12-24 hours to obtain the desired fermented medicine.

2. The novel process for extracting natural Chinese medicine according to claim 1, characterized in that: The moisture content of the fermented raw material after dehydration in step S2 is 15-20%.

3. The novel process for extracting natural Chinese medicine according to claim 1, characterized in that: The particle size of the fermentation raw material after crushing for direct extraction by the supercritical extraction equipment in step S3 is above 200 meshes.

4. The novel process for extracting natural Chinese medicine according to claim 1, characterized in that: The particle size of the fermented raw material after crushing for boiling in the medicine decocting machine in step S3 is above 10 meshes.

5. The novel process for extracting natural Chinese medicine according to claim 1, characterized in that: The bacterial species cultured in step S4 is one or more of Bacillus, yeast, and lactic acid bacteria.

6. The novel process for extracting natural Chinese medicine according to claim 1, characterized in that: When culturing the strains in step S4, the temperature in the tank is maintained at 35-37°C.