A Chinese medicine composition for treating hyperuricemia

Abstract

The invention discloses a Chinese medicine composition for treating hyperuricemia, comprising components of Cyclocarya paliurus, Ligusticum chuanxiong, Gardenia jasminoides, Cichorium indicum, Poria cocos and Licorice. Through processes such as moistening the medicine, freezing and crushing, and fermentation treatment, effective components of the Chinese medicine can be more easily precipitated, while the toxicity of the Chinese medicine composition can be reduced, thereby greatly enhancing the efficacy of the Chinese medicine composition and having a good therapeutic effect on hyperuricemia. At the same time, a multifunctional flocculant is used to perform flocculation treatment on the Chinese medicine composition, thereby effectively reducing the content of ineffective components, improving the stability of the Chinese medicine composition and enhancing its efficacy. The multifunctional flocculant has the advantage of a fast flocculation rate, and can reduce the oxidation and deterioration of effective components of the Chinese medicine during flocculation. The preparation process of the Chinese medicine composition provided by the invention has the characteristics of continuous production and strong efficacy, and is easy for industrial application of the Chinese medicine composition.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating hyperuricemia. Background Art

[0002] Hyperuricemia is a chronic metabolic disease caused by purine metabolism disorder, which can be divided into primary and secondary types. Primary hyperuricemia is mainly caused by reduced uric acid excretion or increased production, while secondary hyperuricemia is caused by certain systemic diseases or taking certain drugs to inhibit uric acid excretion. In addition, eating too much food high in purine may also induce the onset of hyperuricemia. The main clinical symptom of the disease is increased blood uric acid. When it develops into gout, clinical manifestations such as acute arthritis and tophi may occur. Accompanying symptoms are mostly obesity, type 2 diabetes, dyslipidemia, hypertension and arteriosclerosis. At present, the treatment of hyperuricemia mainly includes drug therapy, diet control and lifestyle adjustment. Drug therapy mainly focuses on anti-inflammatory therapy and uric acid-lowering therapy, but the current uric acid-lowering drug therapy has multiple side effects such as intestinal irritation and nephrotoxicity, and there is also a problem of drug resistance. Compared with Western medicine, Chinese medicine has the advantages of low side effects and no drug resistance, and it also has the effect of improving human physique, and has the advantages of safety and wide universality.

[0003] At present, the main method of using Chinese medicine is "taking slices as medicine and decocting medicine for clinical use". Chinese medicine decoction is the most widely used form of clinical Chinese medicine at present, but this method has disadvantages such as inconvenient decoction, easy to mold, low content of effective ingredients and large dosage. Because the decoction requires high temperature and long time, the effective ingredients of Chinese medicine will oxidize and deteriorate during the decoction process. At the same time, due to the long decoction time, starch, mucus, resin, protein, pectin, tannic acid and fiber substances in the medicinal materials will also be extracted. Under normal circumstances, these substances have no pharmacological activity and will reduce the relative content of effective ingredients in the liquid medicine. They are also prone to complexation or quality change during the storage of the liquid medicine, which has a great impact on the clarity, stability and efficacy of the liquid medicine. Therefore, it is necessary to remove impurities and purify the liquid medicine to increase the relative content of effective ingredients in the liquid medicine and ensure the stability and efficacy of the liquid medicine.

[0004] The technology for extracting specific active ingredients from a single medicinal material is relatively mature and can extract the medicinal ingredients of the medicinal material to the maximum extent. However, this technology cannot produce medicine in one step when used for a traditional Chinese medicine composition with multiple Chinese medicinal materials. It has the defect of not being able to be produced continuously. At the same time, this technology has high requirements for equipment, which will increase production costs. Traditional Chinese medicine processing methods such as decoction can be produced continuously, but the traditional Chinese medicine composition prepared by the decoction method has the problem of low content of medicinal ingredients due to poor extraction effect, which makes the traditional Chinese medicine composition poor in efficacy, seriously affecting the promotion and application of the traditional Chinese medicine composition. Summary of the invention

[0005] The first purpose of the present invention is to provide a Chinese medicine composition for treating hyperuricemia.

[0006] The second purpose of the present invention is to provide a process for preparing a Chinese medicine composition which has strong efficacy and can be produced continuously.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention comprises the following steps:

[0008] A traditional Chinese medicine composition for treating hyperuricemia comprises the following components in parts by weight: 20-30 parts of Cyclocarya paliurus, 8-12 parts of Ligusticum chuanxiong, 10-20 parts of Gardenia jasminoides, 30-40 parts of Chicory, 20-30 parts of Poria cocos and 10-14 parts of Licorice.

[0009] Wherein, the specific preparation steps are:

[0010] (1) First, wash the Cyclocarya paliurus, Ligusticum chuanxiong, Gardenia jasminoides, Cichorium indica, Poria cocos and Licorice with water, then put these Chinese medicines into a vacuum medicine moistening machine, moisten them for 10 minutes at a pressure of -0.07 MPa to -0.03 MPa and a temperature of 60 to 65° C., then repeat the medicine moistening process twice, and after the moistening is completed, transfer them into a freeze grinder for freeze grinding to obtain a mixture A;

[0011] (2) adding 6 to 8 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 6 to 8% by volume of the fermentation substrate, and then fermenting at a temperature of 30 to 40° C. and an initial pH of 5.8 to 6.0 for 14 to 16 hours to obtain a fermentation crude liquid;

[0012] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.1 to 0.2 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 100 to 200 r / min for 1 to 2 minutes. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 2500 to 3500 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition, and the preliminary Chinese medicine composition can be made into a preparation.

[0013] Furthermore, the fermentation bacteria seed liquid in step (2) is specifically obtained by mixing Lactobacillus fermentation seed liquid and Aspergillus niger seed liquid in a volume ratio of 1:1;

[0014] Among them, Lactobacillus fermentum and Aspergillus niger are specifically: Lactobacillus fermentum CECT5716 and Aspergillus niger ATCC16404.

[0015] Furthermore, the preparation method of the multifunctional flocculant in step (3) is:

[0016] Immerse 1-2 parts by mass of chitosan in a 12% by mass sodium hydroxide solution, stir for 10-12 hours to obtain a chitosan mixed solution, then add 5-7 parts of the modified liquid to the chitosan mixed solution at a rate of 3-5 drops / min, heat to 65-70°C, stir and react for 10.5-11.5 hours, filter after the reaction is completed to obtain a filter cake, wash the filter cake with deionized water and dry it to obtain a multifunctional flocculant;

[0017] The modified liquid is specifically prepared by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 1-2:50-52:3-5.

[0018] Furthermore, the specific parameters of the freeze-crushing in step (1) are: using liquid nitrogen for freezing, the freezing temperature is -20 to -10°C, the crushing speed is 3000r / min, and the particle size after crushing is 60 to 200 mesh.

[0019] Furthermore, the dosage form of the preparation in step (3) includes granules, decoction, tablets, capsules or oral liquids.

[0020] The Chinese medicinal materials used in the present invention have the following medicinal properties:

[0021] Cyclocarya paliurus is bitter, pungent, neutral in nature and enters the lung and liver meridians. The bark, leaves and roots of Cyclocarya paliurus have the effects of killing insects, relieving itching, reducing inflammation, relieving pain and dispelling wind. The health tea made from the tender leaves of Cyclocarya paliurus in early spring has the function of lowering blood pressure, blood sugar and blood lipids. At the same time, it has a unique effect on enhancing human immunity, anti-oxidation and anti-aging.

[0022] The leaves of Ligustrum lucidum are sweet, flat, non-toxic, and can treat hemorrhoids, stop bleeding and bloody diarrhea, quench thirst, promote blood circulation, and promote urination. They have the effects of lowering blood sugar, lowering blood pressure, regulating blood lipids, and anti-allergy and anti-inflammatory.

[0023] Gardenia is bitter and cold in nature, and enters the heart, liver, lungs, and stomach. It can purge fire and relieve restlessness, clear away heat and dampness, cool blood and detoxify. It can treat symptoms such as hot and painful eyes, severe heat in the chest, heart, large and small intestines, and heat in the stomach.

[0024] Chicory is bitter and cold in nature, and has the effects of clearing away heat and dampness, strengthening the stomach and digesting food. It is mainly used to treat damp-heat jaundice, nephritis edema, stomach pain, and loss of appetite. Its root is the source of the Chinese medicinal material chicory root, which is slightly bitter and cool in nature, and has the effects of clearing away heat and dampness, strengthening the stomach. It is mainly used to treat stomach heat, loss of appetite, chest and abdominal distension, etc.

[0025] Poria cocos is sweet, light and neutral in nature. It enters the heart, lung and kidney meridians, promotes diuresis and eliminates dampness, strengthens the spleen and soothes the mind, calms the heart and improves intelligence. It is mainly used to treat edema, oliguria, phlegm and fluid, dizziness and palpitations, spleen deficiency, poor appetite, loose stools and diarrhea, restlessness, palpitations and insomnia.

[0026] Licorice is sweet and neutral in nature. It enters the spleen, stomach, heart, and lung meridians. It can nourish the spleen and replenish qi, moisten the lungs and relieve cough, harmonize the stomach and relieve urgency, detoxify, and harmonize various medicines. It can treat shortness of breath, abdominal pain, food poisoning, etc.

[0027] The beneficial effects of the present invention are:

[0028] (1) The present invention provides a Chinese medicine composition with Cyclocarya paliurus, Ligusticum chuanxiong, Gardenia jasminoides, Cichorium indicum, Poria cocos and Licorice as components. The Chinese medicine composition has the effects of promoting diuresis and removing dampness, clearing away heat and detoxifying, and strengthening the spleen and stomach. It can effectively promote the excretion of uric acid and reduce the burden on the kidneys. It can also regulate the spleen and stomach, enhance the digestive and absorption functions of the human body, and then enhance the physical fitness and immunity of patients with hyperuricemia. At the same time, it can also reduce the occurrence of inflammation and relieve pain symptoms. The Chinese medicine composition provided in the present application has a good therapeutic effect on hyperuricemia under synergistic effects;

[0029] (2) The present invention performs a process of moistening and freezing the Chinese medicine and, in combination with a subsequent fermentation process, allows the effective components of the Chinese medicine to be more easily precipitated and can also reduce the toxicity of the Chinese medicine composition, thereby greatly enhancing the efficacy of the Chinese medicine composition. The subsequent standing, filtering, flocculation and centrifugation processes can effectively remove the ineffective components in the Chinese medicine composition, thereby increasing the content of the effective components in the Chinese medicine composition, further improving the efficacy and enhancing the stability and clarity of the Chinese medicine composition. At the same time, the filtration and flocculation processes can reduce the viscosity of the medicine residue and improve the centrifugation effect and rate.

[0030] (3) The present invention can obtain a Chinese medicine composition with high content of active ingredients and low content of impurities by extracting and removing impurities from Chinese medicine. The Chinese medicine composition has good therapeutic effect and stability. At the same time, the process method provided by the present invention has the characteristics of one-step preparation, strong process continuity, and can be produced continuously, thus broadening the production and application scope of the Chinese medicine composition;

[0031] (4) After fermentation, the macromolecular substances contained in Chinese medicine will become small molecular substances, which will enhance the efficacy while making these effective ingredients more susceptible to oxidation and deterioration. Therefore, how to shorten the processing cycle is the key to this process. In addition, ineffective ingredients such as starch and mucus in the medicinal materials will also precipitate. These ineffective ingredients will affect the efficacy and stability of the Chinese medicine composition. How to remove impurities without effective effects and increase the content of effective ingredients is another key to this process. The present invention uses a flocculant to remove the ineffective ingredients in the crude fermentation liquid of Chinese medicine. Because the charge density of chitosan will increase significantly under acidic conditions, too high a charge density will affect the bridging effect of chitosan, resulting in difficulty in the growth of flocs, which affects the flocculation effect of chitosan. Therefore, the present invention improves the flocculation effect and flocculation rate of chitosan by modifying the chitosan, shortening the flocculation time, and can have It can effectively reduce the oxidation and deterioration of the active ingredients in the flocculation process, and ensure the efficacy of the liquid medicine. In addition, compared with conventional modifiers such as 2,3-epoxypropyltrimethylammonium chloride, (3-chloro-2-hydroxypropyl) dodecyldimethylammonium chloride can control the charge density of chitosan, thereby enhancing the performance of chitosan flocculation and starch adsorption, and achieve the purpose of improving the stability and clarity of the liquid medicine. At the same time, (3-chloro-2-hydroxypropyl) dodecyldimethylammonium chloride can also effectively enhance the hydrophilicity of chitosan, further improve the flocculation efficiency of chitosan, and shorten the flocculation time. The isopropanol in the modified liquid can improve the water solubility of chitosan, which is beneficial to the modification reaction of chitosan. At the same time, isopropanol can slightly destroy the dense stacking state of chitosan, thereby also achieving the purpose of improving the flocculation efficiency of chitosan, and finally meet the requirements of shortening the construction period and realizing continuous processing. DETAILED DESCRIPTION

[0032] The present invention will be further described in detail below in conjunction with the embodiments.

[0033] The Aspergillus niger ATCC16404 used in the example was purchased from Shenzhen Zike Biotechnology Co., Ltd., and the Lactobacillus fermentum CECT5716 used was purchased from Hangzhou Baosai Biotechnology Co., Ltd.

[0034] Example 1

[0035] The modified liquid was obtained by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 1.5:51:4.

[0036] Immerse 1.5 parts by mass of chitosan in 12% by mass sodium hydroxide solution, stir for 11 hours to obtain a chitosan mixture, then add 6 parts of modified liquid into the chitosan mixture at a rate of 4 drops / min, heat to 67°C and stir for 11 hours, filter after the reaction to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

[0037] (1) First, 25 parts of Cyclocarya paliurus, 10 parts of Ligusticum chuanxiong, 15 parts of Gardenia jasminoides, 35 parts of Chicory, 25 parts of Poria cocos and 12 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.05 MPa and a temperature of 62° C., and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-pulverized for 15 minutes at a temperature of -15° C. and a speed of 3000 r / min, and after pulverization, a mixture A with a particle size of 110 to 130 mesh was obtained;

[0038] (2) adding 7 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 7% by volume of the fermentation substrate, and then fermenting at a temperature of 35° C. and an initial pH of 5.9 for 15 hours to obtain a fermentation crude liquid;

[0039] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.15 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 150 r / min for 1.5 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 3000 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0040] Example 2

[0041] The modified liquid was obtained by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 1:50:5.

[0042] Immerse 1 part by mass of chitosan in 12% by mass sodium hydroxide solution, stir for 10 hours to obtain a chitosan mixture, then add 5 parts of modified liquid into the chitosan mixture at a rate of 5 drops / min, heat to 65°C and stir to react for 11.5 hours. After the reaction is completed, filter to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

[0043] (1) First, 20 parts of Cyclocarya paliurus, 12 parts of Ligusticum chuanxiong, 10 parts of Gardenia jasminoides, 40 parts of Chicory, 20 parts of Poria cocos and 14 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.07 MPa and a temperature of 65°C, and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-crushed for 10 minutes at a temperature of -10°C and a speed of 3000 r / min, and after crushing, a mixture A with a particle size of 150 to 170 mesh was obtained;

[0044] (2) adding 6 parts by weight of water to the mixture A and stirring evenly to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 8% by volume of the fermentation substrate, and then fermenting at a temperature of 30° C. and an initial pH of 5.8 for 16 hours to obtain a fermentation crude liquid;

[0045] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.1 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 200 r / min for 1 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 3500 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0046] Example 3

[0047] The modified liquid was obtained by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 2:52:3.

[0048] Immerse 2 parts of chitosan by mass in 12% sodium hydroxide solution, stir for 12 hours to obtain a chitosan mixture, then add 7 parts of modified liquid into the chitosan mixture at a rate of 3 drops / min, heat to 70°C and stir for 10.5 hours. After the reaction is completed, filter to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

[0049] (1) First, 30 parts of Cyclocarya paliurus, 8 parts of Ligusticum chuanxiong, 20 parts of Gardenia jasminoides, 30 parts of Chicory, 30 parts of Poria cocos and 10 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.03 MPa and a temperature of 60°C, and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-crushed for 20 minutes at a temperature of -20°C and a speed of 3000 r / min, and after crushing, a mixture A with a particle size of 60 to 80 mesh was obtained;

[0050] (2) adding 8 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 8% by volume of the fermentation substrate, and then fermenting at a temperature of 40° C. and an initial pH of 6.0 for 14 hours to obtain a fermentation crude liquid;

[0051] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.2 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 100 r / min for 2 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 2500 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0052] Example 4

[0053] (1) washing 25 parts of Cyclocarya paliurus, 10 parts of Ligusticum chuanxiong, 15 parts of Gardenia jasminoides, 35 parts of Chicory, 25 parts of Poria cocos and 12 parts of Licorice with water, and then transferring these Chinese medicines into a freeze-crusher and freeze-crushing them at a temperature of -15°C and a speed of 3000 r / min for 15 minutes to obtain a mixture A with a particle size of 110 to 130 meshes;

[0054] (2) adding 7 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 7% by volume of the fermentation substrate, and then fermenting at a temperature of 35° C. and an initial pH of 5.9 for 15 hours to obtain a fermentation crude liquid;

[0055] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.15 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 150 r / min for 1.5 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 3000 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0056] The rest is the same as in Example 1.

[0057] Example 5

[0058] (1) First, 25 parts of Cyclocarya paliurus, 10 parts of Ligusticum chuanxiong, 15 parts of Gardenia jasminoides, 35 parts of Chicory, 25 parts of Poria cocos and 12 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.05 MPa and a temperature of 62° C., and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-pulverized for 15 minutes at a temperature of -15° C. and a speed of 3000 r / min, and after pulverization, a mixture A with a particle size of 110 to 130 mesh was obtained;

[0059] (2) adding water to the mixture A to a solid-liquid ratio (g / mL) of 1:10 and decocting twice, each decocting time being 2 h. After decocting, filtering and combining the decoctions;

[0060] (3) adding 0.15 parts by weight of a multifunctional flocculant to the decoction, stirring the decoction at a speed of 150 r / min for 1.5 min, allowing the decoction to settle, centrifuging the decoction at a speed of 3000 r / min, and sterilizing the decoction at high temperature to obtain a preliminary Chinese medicine composition.

[0061] The rest is the same as in Example 1.

[0062] Example 6

[0063] (1) First, 25 parts of Cyclocarya paliurus, 10 parts of Ligusticum chuanxiong, 15 parts of Gardenia jasminoides, 35 parts of Chicory, 25 parts of Poria cocos and 12 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.05 MPa and a temperature of 62° C., and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-pulverized for 15 minutes at a temperature of -15° C. and a speed of 3000 r / min, and after pulverization, a mixture A with a particle size of 110 to 130 mesh was obtained;

[0064] (2) adding 7 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 7% by volume of the fermentation substrate, and then fermenting at a temperature of 35° C. and an initial pH of 6.6 for 15 hours to obtain a fermentation crude liquid;

[0065] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.15 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 150 r / min for 1.5 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 3000 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0066] The rest is the same as in Example 1.

[0067] Test 1:

[0068] Total flavonoids and total polysaccharides were selected as characterization parameters. The total flavonoid content a in the fermentation crude liquid or decoction obtained in some Examples 1 to 6 was determined by spectrophotometry. At the same time, the total polysaccharide content b in the fermentation crude liquid or decoction obtained in Examples 1 to 6 was determined by sulfuric acid-phenol method. The specific data are shown in Table 1.

[0069] Table 1 Total flavonoids and total polysaccharide contents in the fermentation crude liquor or decoction liquor of Examples 1 to 6

[0070] Implementation items Total flavonoid content a(%) Total polysaccharide contentb(%) Example 1 6.67 8.36 Example 2 6.58 8.29 Example 3 6.59 8.31 Example 4 5.82 7.05 Example 5 3.79 5.25 Example 6 6.16 7.72

[0071] It can be seen from Table 1 that the total flavonoids and total polysaccharide contents of Examples 1 to 3 are at the highest level, indicating that the preparation process of the present application can effectively increase the precipitation of effective ingredients of Chinese medicinal materials; the reason for the decrease in the total flavonoids and total polysaccharide contents in Example 4 may be that the drug moistening treatment was not performed before freeze-crushing, and the plant cells were not filled with water, resulting in a worse freeze-crushing effect, which affected the precipitation of effective ingredients of Chinese medicinal materials; the reason for the obvious decrease in the total flavonoids and total polysaccharide contents in Example 5 may be that the fermentation treatment process was replaced by decoction, and the high temperature during the decoction process would make flavonoids and polysaccharides more easily oxidized, and the effective ingredients of Chinese medicine would be less likely to precipitate after the decoction process; the reason for the decrease in the total flavonoids and total polysaccharide contents in Example 6 may be that the initial pH value of the fermentation was high, which was not conducive to the growth of fermented lactobacillus and Aspergillus niger, thereby affecting the fermentation effect.

[0072] Example 7

[0073] The modified liquid was obtained by uniformly mixing 2,3-epoxypropyltrimethylammonium chloride, deionized water and isopropanol in a mass ratio of 1:50:5.

[0074] Immerse 1 part of chitosan by mass in 12% sodium hydroxide solution, stir for 10 hours to obtain a chitosan mixture, then add 5 parts of modified liquid to the chitosan mixture at a rate of 5 drops / min, heat to 78°C and stir for 3 hours, then add 10% hydrochloric acid to adjust the pH value to 7.0, filter, add 100 parts of anhydrous ethanol to the filter cake, stir for 2 hours, filter, place the filter cake in an ultrasonic reactor, add 120 parts of pure water and 15 parts of sodium thiazolinone, select an ultrasonic frequency of 40kHz, react for 2 hours, filter after the reaction is completed to obtain a filter cake, wash the filter cake with deionized water and dry it to obtain a multifunctional flocculant.

[0075] The rest is the same as Example 2.

[0076] Example 8

[0077] The modified liquid was obtained by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride and deionized water in a mass ratio of 1:50.

[0078] Immerse 1 part by mass of chitosan in 12% by mass sodium hydroxide solution, stir for 10 hours to obtain a chitosan mixture, then add 5 parts of modified liquid into the chitosan mixture at a rate of 5 drops / min, heat to 65°C and stir to react for 11.5 hours. After the reaction is completed, filter to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

[0079] The rest is the same as Example 2.

[0080] Example 9

[0081] The modified liquid was obtained by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 1:50:5.

[0082] Immerse 1 part of chitosan by mass in 12% sodium hydroxide solution, stir for 10 hours to obtain a chitosan mixture, then add 5 parts of modified liquid into the chitosan mixture at a rate of 10 drops / min, heat to 65°C and stir for 8 hours, filter after the reaction to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

[0083] The rest is the same as Example 2.

[0084] Example 10

[0085] (1) First, 20 parts of Cyclocarya paliurus, 12 parts of Ligusticum chuanxiong, 10 parts of Gardenia jasminoides, 40 parts of Chicory, 20 parts of Poria cocos and 14 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.07 MPa and a temperature of 65°C, and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-crushed for 10 minutes at a temperature of -10°C and a speed of 3000 r / min, and after crushing, a mixture A with a particle size of 150 to 170 mesh was obtained;

[0086] (2) adding 6 parts by weight of water to the mixture A and stirring evenly to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 8% by volume of the fermentation substrate, and then fermenting at a temperature of 30° C. and an initial pH of 5.8 for 16 hours to obtain a fermentation crude liquid;

[0087] (3) adding 0.1 parts by weight of a multifunctional flocculant to the fermentation crude liquid, stirring the mixture at a speed of 200 r / min for 1 min, allowing the mixture to settle after stirring, centrifuging the mixture at a speed of 3500 r / min, and sterilizing the mixture at high temperature to obtain a preliminary Chinese medicine composition.

[0088] The rest is the same as Example 2.

[0089] Embodiment 11

[0090] (1) First, 20 parts of Cyclocarya paliurus, 12 parts of Ligusticum chuanxiong, 10 parts of Gardenia jasminoides, 40 parts of Chicory, 20 parts of Poria cocos and 14 parts of Licorice were washed with water, and then these Chinese medicines were placed in a vacuum medicine moistening machine, moistened for 10 minutes at a pressure of -0.07 MPa and a temperature of 65°C, and then the medicine moistening process was repeated twice. After the moistening was completed, the medicine was transferred to a freeze crusher and freeze-crushed for 10 minutes at a temperature of -10°C and a speed of 3000 r / min, and after crushing, a mixture A with a particle size of 150 to 170 mesh was obtained;

[0091] (2) adding 6 parts by weight of water to the mixture A and stirring evenly to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 8% by volume of the fermentation substrate, and then fermenting at a temperature of 30° C. and an initial pH of 5.8 for 16 hours to obtain a fermentation crude liquid;

[0092] (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.1 parts by weight of chitosan is added to the filtrate, followed by stirring at a speed of 200 r / min for 1 min. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 3500 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition.

[0093] The rest is the same as Example 2.

[0094] Test 2:

[0095] The starch content c1 in the fermentation crude liquid of Examples 1 to 3 and Examples 7 to 11 and the starch content c2 in the preliminary Chinese medicine composition were determined by dual wavelength determination method, and the starch removal rate was calculated according to the formula C = (c1-c2) / c1*100%. Subsequently, the preliminary Chinese medicine compositions of Examples 1 to 3 and Examples 7 to 11 were placed at 50°C for one month, during which the states of the preliminary Chinese medicine compositions on the sixth, twelfth, eighteenth, twenty-fourth and thirtieth days were observed and recorded, which were recorded as D1, D2, D3, D4 and D5, respectively. The specific data are shown in Table 2.

[0096] Table 2 Stability data of Chinese medicine compositions of Examples 1 to 3 and Examples 7 to 11

[0097]

[0098] It can be seen from Table 2 that the starch removal rates of Examples 1 to 3 are the highest, and the liquid state is still clear, transparent and free of precipitation after being placed at 50°C for one month, indicating that the multifunctional flocculant prepared in the present application has good starch removal and flocculation effects; the reason for the decrease in starch removal rate and liquid stability in Example 7 may be that after (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride is replaced by 2,3-epoxypropyl trimethyl ammonium chloride, on the one hand, the charge density of 2,3-epoxypropyl trimethyl ammonium chloride is larger, which will affect the growth of flocculants and thus affect the flocculation effect, and on the other hand, the hydrophilic enhancement effect of 2,3-epoxypropyl trimethyl ammonium chloride is not as good as that of (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride , which reduces the dispersion effect of the flocculant in the liquid, thereby affecting the flocculation effect; the reason why the data of Example 8 is not as good as that of Example 2 may be that isopropanol is not added to the modified liquid, and isopropanol can promote the modification reaction of chitosan while slightly destroying the tight stacking state of chitosan, thereby improving the flocculation efficiency of chitosan; the reason why the data of Example 9 is not as good as that of Example 2 may be that the modification liquid is added too fast, making the modification reaction uneven, thereby affecting the modification effect; the reason why the data of Example 10 is not as good as that of Example 2 may be that the fermentation crude liquid is not subjected to static filtration treatment, and the solid matter in the fermentation crude liquid will affect the flocculation effect of the flocculant; the reason why the data of Example 11 is not as good as that of Example 2 is that the multifunctional flocculant is replaced with chitosan.

[0099] Test 3:

[0100] 130 SD rats were randomly divided into 13 groups, each group of 10 rats, respectively, a blank group, a model group and Examples 1 to 11 each group, during the experiment, except for the blank group, the remaining groups of animals were gavaged with adenine (100 mg / kg) and potassium oxonate (300 mg / kg) suspension (the solvent was 0.5% sodium carboxymethyl cellulose aqueous solution) every day, the blank group was given the same volume of 0.5% sodium carboxymethyl cellulose aqueous solution, and the administration was continued for 28 days. From the 7th day after the successful modeling, the Chinese medicine composition prepared by Examples 1 to 11 was administered at a dosage of 1 mL / kg every day, and the blank group and the model group were given 1 mL / kg of pure water every day, and sampling was performed after 7 days of administration. The concentration of serum uric acid in rats of each group was measured, and the obtained values ​​were averaged. The above test results are shown in Table 3.

[0101] Table 3 Serum uric acid concentration data of rats in each group

[0102] Implementation items Uric acid concentration (μmol / L) Example 1 134.5 Example 2 134.8 Example 3 135.1 Example 4 155.3 Example 5 182.7 Example 6 148.6 Example 7 154.2 Example 8 141.5 Example 9 143.1 Example 10 140.2 Embodiment 11 161.4 Blank Group 121.2 Model Group 243.8

[0103] It can be seen from Table 3 that the Chinese medicine composition liquid prepared in Examples 1 to 3 can effectively reduce the uric acid content in rats, indicating that the Chinese medicine composition liquid prepared in the present application has a good therapeutic effect on hyperuricemia; Examples 4 to 6 have a lower content of active ingredients in the liquid and thus a worse therapeutic effect; the reason why the therapeutic effect of Examples 7 to 11 is worse is that, on the one hand, the flocculant effect is poor, the removal rate of the original impurities in the liquid is low, and the higher the impurity content, and on the other hand, the flocculation rate is low, resulting in the deterioration of the active ingredients, and then the content of the active ingredients in the liquid is correspondingly lower, which makes the therapeutic effect worse.

Claims

1. A Chinese medicine composition for treating hyperuricemia, characterized in that: The invention comprises the following components in parts by weight: 20 to 30 parts of Cyclocarya paliurus, 8 to 12 parts of Ligusticum chuanxiong, 10 to 20 parts of Gardenia jasminoides, 30 to 40 parts of Chicory, 20 to 30 parts of Poria cocos and 10 to 14 parts of Licorice.

2. A Chinese medicine composition for treating hyperuricemia according to claim 1, characterized in that: The specific preparation steps are: (1) First, wash the Cyclocarya paliurus, Ligusticum chuanxiong, Gardenia jasminoides, Cichorium indica, Poria cocos and Licorice with water, then put these Chinese medicines into a vacuum medicine moistening machine, moisten them for 10 minutes at a pressure of -0.07 MPa to -0.03 MPa and a temperature of 60 to 65° C., then repeat the medicine moistening process twice, and after the moistening is completed, transfer them into a freeze grinder for freeze grinding to obtain a mixture A; (2) adding 6 to 8 parts by weight of water to the mixture A and stirring to obtain a fermentation substrate, then adding a fermentation bacteria seed solution of 6 to 8% by volume of the fermentation substrate, and then fermenting at a temperature of 30 to 40° C. and an initial pH of 5.8 to 6.0 for 14 to 16 hours to obtain a fermentation crude liquid; (3) After the fermented crude liquid is allowed to stand, it is filtered to obtain a filtrate, and then 0.1 to 0.2 parts by weight of a multifunctional flocculant is added to the filtrate, followed by stirring at a speed of 100 to 200 r / min for 1 to 2 minutes. After the stirring is completed, it is allowed to stand and settle, and then centrifuged at a speed of 2500 to 3500 r / min, and then sterilized at high temperature to obtain a preliminary Chinese medicine composition, and the preliminary Chinese medicine composition can be made into a preparation.

3. A Chinese medicine composition for treating hyperuricemia as claimed in claim 2, characterized in that: The preparation method of the multifunctional flocculant is: Immerse 1-2 parts by mass of chitosan in 12% by mass sodium hydroxide solution, stir for 10-12 hours to obtain a chitosan mixed solution, then add 5-7 parts of modified liquid to the chitosan mixed solution at a rate of 3-5 drops / min, heat to 65-70°C and stir to react for 10.5-11.5 hours. After the reaction is completed, filter to obtain a filter cake, wash the filter cake thoroughly with deionized water and dry it to obtain a multifunctional flocculant.

4. A Chinese medicine composition for treating hyperuricemia as claimed in claim 3, characterized in that: The modified liquid is specifically: The preparation method is prepared by uniformly mixing (3-chloro-2-hydroxypropyl) dodecyl dimethyl ammonium chloride, deionized water and isopropanol in a mass ratio of 1-2:50-52:3-5.

5. A Chinese medicine composition for treating hyperuricemia as claimed in claim 2, characterized in that: The fermentation bacteria seed liquid is specifically obtained by mixing fermentation lactobacillus seed liquid and aspergillus niger seed liquid in a volume ratio of 1:

1.

6. A Chinese medicine composition for treating hyperuricemia as claimed in claim 2, characterized in that: The specific parameters of the freezing and pulverizing are: using liquid nitrogen for freezing, the freezing temperature is -20 to -10°C, the pulverizing speed is 3000r / min, and the particle size after pulverization is 60 to 200 meshes.

7. A Chinese medicine composition for treating hyperuricemia as claimed in claim 2, characterized in that: The dosage form of the preparation includes granules, decoction, tablets, capsules or oral liquid.

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