Plant composition with fat reducing effect and preparation method of fermentation extract of plant composition

Abstract

The invention discloses a plant composition with a fat reducing effect and a preparation method of a fermentation extract of the plant composition. Mangnolia officinalis, pepper, astragalus membranaceus, honeysuckle, salvia miltiorrhiza and immature bitter orange are combined together according to the compatibility of monarch, minister, assistant and guide to obtain an extract fermented by yeast, polyphenol components of the extract are enriched after impurity removal and purification, total polyphenol and soluble solids are taken as index contents, the extract can be prepared into an external fat reducing agent for use, qi and blood circulation of a body is facilitated, fat metabolism is regulated, and the fat reducing effect is improved. And the tea is safe and mild, and has the effects of reducing fat, losing weight, slimming, shaping and delaying senescence.

Description

Technical Field

[0001] The present invention relates to the technical field of plant combination fermentation extraction and application in the field of health, and in particular to the preparation and application of a fat-reducing plant combination fermentation extract. Background Art

[0002] Obesity is a complex, multi-factorial metabolic disorder characterized by excessive accumulation of body fat. It is caused by the disruption of the balance between food intake and energy expenditure. It not only affects people's appearance, but is also closely related to the occurrence of diabetes, fatty liver, hypertension and various cardiovascular diseases. The problem of obesity has obvious effects on people's physical and mental health. Currently, common weight loss methods include dieting, exercise, surgery, oral medication, etc. However, dieting has the disadvantage of reducing the body's immunity, exercise has the disadvantage of being prone to injury and rebound, and surgery and oral medication have unavoidable medical risks and adverse reaction risks. Therefore, finding a safe and efficient fat reduction plan has become a hot social health issue of widespread concern today.

[0003] In the thousands of years of development, Chinese medicine has formed a systematic theory and accumulated rich experience in treating obesity. However, due to the complexity and uncertainty of the study of its pharmacological effects caused by the diversity of plant origins and ingredients, the promotion and application of plant prescriptions for fat reduction and weight loss are limited. This is far from the prevention and treatment of obesity and the actual needs of the majority of patients. Plant polyphenols are the most diverse type of secondary metabolites in plants. They are widely present in various parts of plants, and polyphenols are reported to have multiple biological activities such as antioxidant, anti-inflammatory, antibacterial, antiviral and anti-cancer. A large number of studies have shown that plant polyphenols can regulate fat metabolism, affect lipid digestion, absorption, synthesis and decomposition, promote lipid efflux, inhibit the digestion, absorption and transport of carbohydrates, stimulate the body to produce heat to accelerate energy consumption, thereby playing a role in reducing fat and weight loss. They have a good effect in preventing or treating obesity and its complications, and have become a research hotspot at home and abroad. Summary of the invention

[0004] In order to solve the problems of the prior art, the present invention provides a preparation and application of a fat-reducing plant combination fermentation extract, wherein Magnolia officinalis, Zanthoxylum bungeanum, Astragalus membranaceus, Honeysuckle, Salvia miltiorrhiza, and Citrus aurantium are combined according to the principle, minister, assistant, and guiding compatibility to obtain an extract prepared by yeast fermentation, and the polyphenol components of the extract are enriched after impurities and purification. The total polyphenols and soluble solids are used as indicator contents, and the extract can be made into an external fat-reducing agent for use, which is helpful for the circulation of qi and blood in the body, regulates fat metabolism, and promotes the elimination of metabolic waste and toxins in the body. The extract is safe and mild, and has the effects of reducing fat and weight, slimming and shaping the body, and delaying aging.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A plant composition consists of the following components in parts by mass: 50-300 parts of magnolia bark, 50-300 parts of zanthoxylum bungeanum, 20-200 parts of astragalus, 20-200 parts of honeysuckle, 20-100 parts of salvia miltiorrhiza, and 10-80 parts of immature bitter orange.

[0007] A plant combination fermentation extract is an initial system obtained by mixing the aforementioned plant combination and fermentation bacteria, adding 2.5 times the weight of pure water of the plant combination, and placing the fermented product obtained by fermentation in a decoction machine, adding 20 times the weight of pure water of the plant combination, extracting at 95-100°C for 1.5h, and filtering after impurity removal and purification to obtain the extract.

[0008] The fermentation bacteria is Saccharomyces cerevisiae, and in the fermentation step, the temperature is 28-42°C, the stirring speed is 0-50rpm, and the time is 16-72h. The impurity removal and purification method is biological flocculation, and the flocculants used are modified chitosan and carrageenan.

[0009] The index contents of the plant combination fermentation extract include the following two items: total polyphenols ≥ 1.5 mg / mL, soluble solid content ≥ 5.0%.

[0010] The plant combination fermentation extract is used to be added into external preparation products at a weight percentage of 0.01%-100%. The external preparation products used include: medicines, medical devices, cosmetics, and daily necessities.

[0011] The main effects of the components of the plant composition are as follows: Magnolia officinalis can promote qi and relieve bloating, dry out dampness and eliminate fullness, and help digestion and relieve stagnation. The polyphenolic compounds magnolol and honokiol contained in it have significant weight loss effects, which can activate the brown adipose tissue in the body, increase energy consumption, promote fat decomposition and metabolism, and can also suppress appetite and reduce food intake, thereby achieving the effect of weight loss. At the same time, they have antioxidant and anti-inflammatory effects, can reduce chronic inflammatory reactions caused by obesity, and improve insulin sensitivity; Zanthoxylum bungeanum can dispel dampness and cold, and help improve local blood circulation and metabolism. The polyphenolic compound caffeic acid contained in it can regulate the activity of enzymes related to fat metabolism, promote the decomposition of fat into glycerol and fatty acids, increase the oxidative energy supply of fatty acids, and can also inhibit the absorption of fat by the gastrointestinal tract, thereby reducing Reduces the accumulation of fat in the body; Astragalus invigorates the spleen and replenishes the middle, raises yang and lifts the sunken; the polyphenol compound anthocyanin contained in it has multiple phenolic hydroxyl groups and has strong antioxidant properties, which can suppress appetite and reduce food intake; Honeysuckle clears away heat and reduces swelling; the polyphenol compound chlorogenic acid contained in it can inhibit the synthesis of lipids in fat cells, reduce the synthesis of fatty acids by regulating the activity of enzymes related to fat metabolism, and accelerate the hydrolysis of triglycerides in fat cells to promote the oxidative metabolism of fat; Salvia miltiorrhiza promotes blood circulation, removes blood stasis, promotes menstruation and relieves pain; the polyphenol compound salvianolic acid B contained in it can reduce fatty acid synthesis by regulating the activity of enzymes related to lipid metabolism; Citrus aurantium breaks up qi and eliminates accumulation; the polyphenol compound hesperidin contained in it can promote fatty acid oxidation and glucose uptake and utilization, and accelerate fat metabolism.

[0012] The beneficial effects brought by the technical solution provided by the present invention are:

[0013] The present invention combines Magnolia officinalis, Zanthoxylum bungeanum, Astragalus membranaceus, Honeysuckle, Salvia miltiorrhiza and Citrus aurantium in the form of monarch, minister, assistant and guiding compatibility to obtain an extract prepared by yeast fermentation, which is enriched with polyphenol components after fermentation of the plant composition and can be made into an external fat-reducing agent for use, which is helpful for the circulation of Qi and blood in the body, regulates fat metabolism, promotes the elimination of metabolic waste and toxins in the body, is safe and mild, and has the effects of reducing fat and weight, slimming and shaping the body, and delaying aging.

[0014] The fat-reducing plant combination fermented extract of the present invention has a wide range of applications, is safe and effective, can be used for a long time, has good compatibility, has a simple process for adding it to an external preparation, and is suitable for industrial production. DETAILED DESCRIPTION

[0015] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention will be described in further detail below in conjunction with specific implementation methods.

[0016] Example 1: Preparation of plant combination fermentation extract

[0017] Weigh 300g of Magnolia officinalis, 300g of Zanthoxylum bungeanum, 20g of Astragalus, 20g of Honeysuckle, 20g of Salvia miltiorrhiza, and 10g of Citrus aurantium, wash and place in a fermentation tank, add 85g of brewer's yeast and 1.675kg of pure water, ferment for 48h at 35°C and a stirring speed of 5rpm, place the material in a decoction machine, add 13.4kg of pure water, extract at 95-100°C for 1.5h, stand for 2h and take the supernatant for use. Prepare 1.5% modified chitosan aqueous solution and 1.2% carrageenan aqueous solution, add 10% of the weight of the supernatant modified chitosan aqueous solution, stand for 30min and then slowly add the carrageenan aqueous solution until flocculent precipitate appears and the supernatant becomes clear, take the clarified liquid and filter it at 0.22 microns, fill and seal, and obtain the plant combination fermentation extract. The detection method and results are shown in Table 1:

[0018]

[0019] Table 1. Index content detection

[0020] Example 2: Preparation of plant combination fermentation extract

[0021] Weigh 50g of Magnolia officinalis, 50g of Zanthoxylum bungeanum, 200g of Astragalus, 200g of Honeysuckle, 100g of Salvia miltiorrhiza, and 80g of Citrus aurantium, wash and place in a fermentation tank, add 86g of brewer's yeast and 1.7kg of pure water, ferment for 36h at 38°C and a stirring speed of 10rpm, place the material in a decoction machine, add 13.6kg of pure water, extract at 95-100°C for 1.5h, stand for 2h and take the supernatant for use. Prepare 1.5% modified chitosan aqueous solution and 1.2% carrageenan aqueous solution, add 9% modified chitosan aqueous solution by weight of the supernatant, stand for 30min and slowly add carrageenan aqueous solution until flocculent precipitate appears and the supernatant becomes clear, take the clarified liquid and filter it at 0.22 microns, fill and seal, and obtain the plant combination fermentation extract. The detection method and results are shown in Table 2:

[0022]

[0023] Table 2. Index content detection

[0024] Example 3: Application of plant combination fermented extract in fat-reducing cream

[0025] The plant combination fermentation extract prepared in Example 1 was used as the raw material of phase C and added to the fat-reducing cream in Table 3.

[0026]

[0027] Table 3

[0028] The components in the formula of Table 3 are prepared according to the following process:

[0029] (1) First, weigh each component according to the component name and weight in the above table;

[0030] (2) Mix and stir the components of phase A and heat to 80-85°C to dissolve completely;

[0031] (3) Mix and stir the components of phases B and C, and heat to 82-87°C to dissolve completely;

[0032] (4) Add phase A to phase B, homogenize at high speed for 5 minutes, and then stir at 80-85°C for 30 minutes;

[0033] (5) When the temperature of the emulsified system slowly drops to 60°C, add phase D and stir evenly;

[0034] (6) When the temperature of the emulsified system drops below 40°C, discharge the material.

[0035] Example 4: Application of plant combination fermented extract in fat-reducing essence

[0036] The plant combination fermentation extract prepared in Example 2 was used as the phase B raw material and added to the fat-reducing essence in Table 4.

[0037]

[0038] Table 4

[0039] The components in the formula of Table 4 are prepared according to the following process:

[0040] (1) First, weigh each component according to the component name and weight in the above table and put it into a stirring pot;

[0041] (2) Mix and stir the eight components in phase A until they are completely dissolved;

[0042] (3) Add phase B with stirring until all components are evenly mixed and completely dissolved, then discharge.

[0043] The clinical treatment observations of the present invention are as follows:

[0044] 1. The obese patients in the treatment group included 12 males and 13 females, aged 25-50 years old; the obese patients in the control group included 12 males and 13 females, aged 22-49 years old. Part of application: abdomen.

[0045] 2. Diagnostic criteria for obesity:

[0046] Body mass index (BMI) = weight (kg) / height 2 (m 2 ). Normal body mass index: 18.5≤BMI≤23.9, overweight range: 24≤BMI≤27.9, obesity range: BMI≥28.

[0047] 3. Diagnostic criteria for central obesity:

[0048] Waist circumference (WC) ≥ 85 cm for men and ≥ 80 cm for women; waist-to-hip ratio (WHR) = waist circumference / hip circumference, WHR ≥ 0.90 for men and ≥ 0.85 for women; BMI ≥ 24. Patients who meet at least 2 of the criteria can be diagnosed.

[0049] Treatment group: The fat-reducing cream prepared in Example 3 with the plant combination fermentation extract added was selected. The patient was placed in a supine position, with the abdomen fully exposed. The sample was evenly applied to the upper and lower boundaries of the abdomen. The upper boundary was marked by the xiphoid process, the costal arch, the front end of the 11th rib, and the lower edge of the 12th rib. The lower boundary was marked by the upper edge of the pubic symphysis, the pubic tubercle, the inguinal ligament, and the anterior part of the iliac crest. The sample was naturally absorbed after application. During the treatment period, the sample was applied once in the morning and evening, with a dosage of 8-10 g each time. One course of treatment was defined as 2 months of continuous use.

[0050] Control group: the cream without adding plant combination fermentation extract (replaced by water) prepared by the same formula process as Example 3 was selected. The patient was in supine position, fully exposed the abdomen, and pure water was evenly applied to the upper and lower boundaries of the abdomen. The upper boundary was marked by the xiphoid process, the costal arch, the front end of the 11th rib, and the lower edge of the 12th rib. The lower boundary was marked by the upper edge of the pubic symphysis, the pubic tubercle, the inguinal ligament and the anterior part of the iliac crest. After application, it was naturally absorbed. During the treatment period, it was applied once in the morning and evening every day, with a dosage of 8-10g each time, and 2 months of continuous use was a course of treatment.

[0051] 4. Observation Indicators

[0052] The body weight (kg), waist circumference (cm), abdominal circumference (cm), and hip circumference (cm) of the patients before and after treatment were observed, and BMI and WHR were calculated.

[0053] V. Efficacy evaluation criteria

[0054] Markedly effective: weight loss ≥5kg or average abdominal circumference reduction ≥10cm; effective: 5kg>weight loss ≥2kg or 10cm>average abdominal circumference reduction ≥3cm; ineffective: weight loss <2kg or average abdominal circumference reduction <3cm.

[0055] 6. Treatment Effect

[0056] The data were averaged and the waist circumference was compared, indicating that the treatment group was able to reduce the waist circumference more than the control group. The results are shown in Table 5:

[0057] Group Before treatment (cm) After treatment (cm) Difference(cm) Treatment Group 102.61 96.37 6.24 Control group 102.86 101.74 1.12

[0058] Table 5

[0059] The data were averaged and the waist-to-hip ratio was compared, indicating that the treatment group was able to reduce the waist-to-hip ratio more than the control group. The results are shown in Table 6:

[0060] Group Before treatment After treatment Difference Treatment Group 1.01 0.83 0.18 Control group 0.99 0.95 0.04

[0061] Table 6

[0062] Comparison of the clinical efficacy between the two groups showed that the effective rate of the treatment group was significantly better than that of the control group. The results are shown in Table 7:

[0063] Group Number of cases Significant effect efficient invalid Effectiveness% Treatment Group 25 7 14 4 84 Control group 25 0 3 22 12

[0064] Table 7

[0065] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant composition having fat-reducing effect, characterized in that: The plant composition consists of the following components in parts by mass: 50-300 parts of magnolia bark, 50-300 parts of prickly ash, 20-200 parts of astragalus, 20-200 parts of honeysuckle, 20-100 parts of salvia miltiorrhiza, and 10-80 parts of immature bitter orange.

2. The plant composition according to claim 1, characterized in that The components specifically include: 300 parts of Magnolia officinalis, 300 parts of Zanthoxylum bungeanum, 20 parts of Astragalus, 20 parts of Honeysuckle, 20 parts of Salvia miltiorrhiza, and 10 parts of Citrus aurantium.

3. The plant composition according to claim 1, characterized in that The components specifically include: 50 parts of Magnolia officinalis, 50 parts of Zanthoxylum bungeanum, 200 parts of Astragalus, 200 parts of Honeysuckle, 100 parts of Salvia miltiorrhiza, and 80 parts of Citrus aurantium.

4. A method for preparing a fermented extract of the plant composition according to claim 1, 2 or 3, characterized in that: The initial system is obtained by mixing the plant composition and fermentation bacteria, adding 2.5 times the weight of pure water of the plant composition, placing the fermented product in a decoction machine, adding 20 times the weight of pure water of the plant composition, extracting at 95-100°C for 1.5h, and filtering after impurity removal and purification to obtain the product.

5. The method for preparing the fermentation extract according to claim 4, characterized in that: The fermentation bacteria is Saccharomyces cerevisiae. In the fermentation step, the temperature is 28-42°C, the stirring speed is 0-50 rpm, and the time is 16-72 hours.

6. The method for preparing the fermentation extract according to claim 4, characterized in that: The impurity removal and purification method is biological flocculation, and the flocculants used are modified chitosan and carrageenan. After impurity removal and purification, the polyphenol components of the extract are enriched.

7. A method for preparing a fermentation extract according to claim 5, characterized in that: The total polyphenol content in the fermentation extract is ≥1.5 mg / mL, and the soluble solid content is ≥5.0%.

8. A method for preparing a fermentation extract according to claim 6, characterized in that: The total polyphenol content in the fermentation extract is ≥1.5 mg / mL, and the soluble solid content is ≥5.0%.

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