Plant composition with weight losing function and preparation method of fermentation extract of plant composition

Abstract

The invention discloses a plant composition with a weight losing function and a preparation method of a fermentation extract of the plant composition. The lavender, the dogwood fruits, the pepper fruits and the rheum palmatum roots and stems are combined according to the compatibility of monarch, minister, assistant and guide and are fermented with saccharomycetes to obtain an extract, flavone components of the extract are enriched after impurity removal and purification, total flavonoids and soluble solids are used as index contents, and the extract can be prepared into an external weight-losing agent for use, has the functions of losing weight, slimming, resisting aging and the like, and can be used as a weight-losing agent. The fat-reducing tea is beneficial to promoting catabolism of lipid, regulating the blood fat level, eliminating metabolic wastes and toxins in vivo and reducing fat accumulation, so that the weight-reducing effect is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant combination fermentation extraction and application in the field of health, and in particular to the preparation and application of a weight-loss plant combination fermentation extract. Background Art

[0002] Obesity is caused by the body's energy intake exceeding energy loss and long-term energy imbalance, which is specifically manifested as excessive accumulation or uneven distribution of fat in the body. The formation of overweight or obesity is a long and complex process, which is affected by genetic, social and environmental factors, and is closely related to dietary structure, physical activity, metabolic intensity, intestinal flora, age of the population and even social status. Therefore, it is of positive significance to explore treatment options for obesity. At present, oral Western medicine and surgical treatment of obesity have problems such as poor efficacy, large side effects, and trauma. Traditional Chinese medicine has obvious advantages in the diagnosis and treatment of obesity. External Chinese medicine is mainly absorbed through the skin into the blood circulation to play a role in intervening in obesity, without hurting the internal organs. It has the advantages of being simple and easy, with little toxicity and side effects, no pain, and strong comfort. At present, the search for natural, safe, convenient and effective methods of weight loss and fat reduction has received widespread attention from the whole society.

[0003] Flavonoids are one of the most abundant secondary metabolites in plants, and are found in almost all parts of plants, including roots, stems, skins, leaves, fruits, and flowers. Flavonoids from leaves, flowers, and fruits usually exist in the form of glycosides. Modern studies have shown that flavonoids have the effects of reducing appetite, reducing lipase activity, inhibiting fat absorption, inhibiting fat synthesis, and promoting energy metabolism in the body, thus promoting weight loss and fat burning. In addition, as a natural biological antioxidant, flavonoids can effectively remove excess free radicals in the body, reduce oxidative stress, digest fat, and control body weight, which is of great significance for the prevention and treatment of obesity. Summary of the invention

[0004] In order to solve the problems of the prior art, the present invention provides the preparation and application of a fermented extract of a weight-loss plant combination, wherein lavender, cornus officinalis fruit, Zanthoxylum bungeanum fruit, and Rheum palmatum rhizome are combined in a monarch, minister, assistant, and guiding combination and fermented with yeast to obtain an extract, and the flavonoid components of the extract are enriched after impurities are removed and purified, and the total flavonoids and soluble solids are used as index contents. The extract can be made into an external weight-loss agent for use, and has the functions of weight loss, slimming, anti-aging, etc., and is helpful to promote lipid decomposition metabolism, regulate blood lipid levels, eliminate metabolic waste and toxins in the body, and reduce fat accumulation to achieve a weight-loss effect.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A plant composition consists of the following components in parts by mass: 50-200 parts of lavender, 100-200 parts of cornus officinalis fruits, 20-200 parts of Zanthoxylum bungeanum fruits, and 20-100 parts of palm-leaved rhubarb root stalks.

[0007] A plant combination fermentation extract is an initial system obtained by mixing the aforementioned plant combination and fermentation bacteria, adding pure water 3 times the weight of the plant combination, and placing the fermented product obtained by fermentation in a decoction machine, adding pure water 20 times the weight of the plant combination, extracting at 95-100°C for 1.5 hours, and filtering after impurity removal and purification to obtain the extract.

[0008] The fermentation bacteria is Saccharomyces cerevisiae, and in the fermentation step, the temperature is 30-40°C, the stirring speed is 0-50rpm, and the time is 16-48h. The impurity removal and purification method is biological flocculation, and the flocculants used are water-soluble chitosan and carrageenan.

[0009] The index contents of the plant combination fermentation extract include the following two items: total flavonoids ≥ 1.7 mg / mL, soluble solid content ≥ 4.8%.

[0010] The plant combination fermentation extract is used to be added into external preparation products at a weight percentage of 0.01%-100%. The external preparation products used include: medicines, medical devices, cosmetics, and daily necessities.

[0011] The main effects of the components of the plant composition are as follows: the flavonoid compounds luteolin and apigenin in lavender have weight loss effects. Luteolin can reduce the number of fat cells by inhibiting the signal pathways related to the differentiation and proliferation of fat cells, and promote the decomposition and metabolism of lipids in fat cells. Apigenin can enhance the cell's perception and utilization of energy, promote the uptake and oxidation of fatty acids, and reduce fat synthesis, thereby playing a weight loss role; Cornus officinalis fruit nourishes the liver and kidneys, astringes and consolidates the body, and the flavonoid compounds kaempferol and quercetin contained therein have the effects of reducing the synthesis of fatty acids and reducing the number of fat cells; the flavonoid compound rutin contained in Zanthoxylum bungeanum fruit can inhibit lipid absorption, regulate blood lipid levels, and reduce fat accumulation; the flavonoid compound isorhamnetin contained in the rhizome of Rheum palmatum can accelerate the decomposition and utilization of fat, increase energy consumption, and thus reduce the accumulation of fat in the body.

[0012] The beneficial effects brought by the technical solution provided by the present invention are:

[0013] The invention combines lavender, cornus officinalis fruit, Zanthoxylum bungeanum fruit and rhubarb root stalk according to the principle, minister, assistant and guiding compatibility to obtain the extract prepared by yeast fermentation, and the flavonoid components of the plant composition after fermentation are enriched, which can be made into an external slimming agent for use, has the functions of slimming, body weight loss and anti-aging, and is helpful to promote lipid decomposition metabolism, regulate blood lipid levels, eliminate metabolic waste and toxins in the body, reduce fat accumulation and thus achieve the slimming effect.

[0014] The weight-loss plant combination fermented extract of the present invention has a wide application range, is safe and effective, can be used for a long time, has good compatibility, has a simple process for adding it to an external preparation, and is suitable for industrial production. DETAILED DESCRIPTION

[0015] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention will be described in further detail below in conjunction with specific implementation methods.

[0016] Example 1: Preparation of plant combination fermentation extract

[0017] Weigh 200g of lavender, 200g of cornus fruit, 20g of prickly ash fruit, and 20g of palm leaf rhubarb root stalk, wash and place in a fermentation tank, add 69g of brewer's yeast and 1.32kg of pure water, ferment for 36h at 35°C and a stirring speed of 5rpm, place the material in a decoction machine, add 8.8kg of pure water, extract at 95-100°C for 1.5h, stand for 2h and take the supernatant for use. Prepare 1.5% water-soluble chitosan aqueous solution and 1.2% carrageenan aqueous solution, add 9.3% water-soluble chitosan aqueous solution by weight of the supernatant, stand for 30min and slowly add carrageenan aqueous solution until flocculent precipitate appears and the supernatant becomes clear, take the clear liquid and filter it at 0.22 microns, fill and seal, and obtain the plant combination fermentation extract. The detection method and results are shown in Table 1:

[0018]

[0019] Table 1. Index content detection

[0020] Example 2: Preparation of plant combination fermentation extract

[0021] Weigh 50g of lavender, 100g of cornus fruit, 200g of prickly ash fruit, and 100g of palm leaf rhubarb root stalk, wash and place in a fermentation tank, add 71g of brewer's yeast and 1.35kg of pure water, ferment for 24h at 36°C and a stirring speed of 10rpm, place the material in a decoction machine, add 9kg of pure water, extract at 95-100°C for 1.5h, stand for 2h and take the supernatant for use. Prepare 1.5% water-soluble chitosan aqueous solution and 1.2% carrageenan aqueous solution, add 9.6% water-soluble chitosan aqueous solution by weight of the supernatant, stand for 30min and then slowly add carrageenan aqueous solution until flocculent precipitate appears and the supernatant becomes clear, take the clear solution and filter it at 0.22 microns, fill and seal, and obtain the plant combination fermentation extract. The detection method and results are shown in Table 2:

[0022]

[0023] Table 2. Index content detection

[0024] Example 3: Application of plant combination fermented extract in weight loss milk

[0025] The plant combination fermentation extract prepared in Example 1 was used as the phase C raw material and added to the weight loss milk in Table 3.

[0026]

[0027]

[0028] Table 3

[0029] The components in the formula of Table 3 are prepared according to the following process:

[0030] (1) First, weigh each component according to the component name and weight in the above table;

[0031] (2) Mix and stir the components of phase A and heat to 80-85°C to dissolve completely;

[0032] (3) Mix and stir the components of phases B and C, and heat to 82-87°C to dissolve completely;

[0033] (4) Add phase A to phase B, homogenize at high speed for 5 minutes, and then stir at 80-85°C for 30 minutes;

[0034] (5) When the temperature of the emulsified system drops below 40°C, discharge the material.

[0035] Example 4: Application of plant combination fermented extract in weight loss toner

[0036] The plant combination fermentation extract prepared in Example 2 was used as the phase B raw material and added to the weight loss toner in Table 4.

[0037]

[0038] Table 4

[0039] The components in the formula of Table 4 are prepared according to the following process:

[0040] (1) First, weigh each component according to the component name and weight in the above table and put it into a stirring pot;

[0041] (2) Mix and stir the components in phase A until they are completely dissolved;

[0042] (3) Add phase B with stirring until all components are evenly mixed and completely dissolved, then discharge the material.

[0043] Efficacy verification of the present invention:

[0044] Preparation of experimental obesity animal model: 60 SD rats were fed with nutritious feed (composition: 10% milk powder, 10% lard, 10% egg yolk powder, 70% basic feed), and randomly divided into two groups after 6 weeks of modeling to start the experiment. During the experiment, all animals were given free drinking water and had normal day and night rhythms.

[0045] Treatment group: 30 rats, selected the slimming milk prepared in Example 3 with added plant combination fermentation extract, took 0.5g and applied it on 2×2cm 2 Apply massage to the surface of abdomen for a while and then absorb it naturally. Do this twice a day for 6 weeks.

[0046] Control group: 30 rats, using the emulsion prepared by the same formula and process as in Example 3 without adding the plant combination fermentation extract (replaced with water), 0.5g was applied to a 2×2cm 2 Apply massage to the surface of abdomen for a while and then absorb it naturally. Do this twice a day for 6 weeks.

[0047] Body weight and obesity index (Lee's index): At the end of the experiment, the body weight and body length (the length from the nose to the anus of the rat was measured after ether anesthesia) were measured to calculate the Lee's index. Calculation formula:

[0048]

[0049] Serum biochemical indices: 3 mL of blood was collected from the orbital vein after ether anesthesia, and the serum was separated. The contents of TC, TG, HDL-C, and LDL-C in the serum were determined using an automatic biochemical analyzer.

[0050] Treatment Effect:

[0051] Effects on the weight, length and Lee's index of obese rats. The results are shown in Table 5:

[0052] Group Body weight before experiment (g) Body weight after experiment (g) Body length after experiment (cm) Lee's Index Treatment Group 250.52±18.91 315.70±45.35 22.29±1.25 305±17.12 Control group 251.03±21.05 371.30±52.65 22.11±1.12 325±16.46

[0053] Table 5

[0054] The results showed that compared with the control group, the treatment group could control the weight gain of the obese animal model fed with a high-fat diet and significantly reduce the Lee's index.

[0055] Effects on fat metabolism in obese rats. The results are shown in Table 6:

[0056] Group TC(mmol / L) TG (mmol / L) HDL-C (mmol / L) LDL-C (mmol / L) Treatment Group 1.39±0.15 0.79±0.19 0.48±0.06 0.41±0.12 Control group 1.64±0.28 1.18±0.20 0.47±0.06 0.49±0.16

[0057] Table 6

[0058] The results showed that compared with the control group, the samples in the treatment group could reduce the levels of TC, TG, and LDL in obese mice. The three are important indicators of lipid metabolism in the body. The increase in their levels is the objective basis of hyperlipidemia and one of the main causes of obesity, indicating that the samples in the treatment group have a certain lipid-lowering effect.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant composition with weight loss function, which is composed of the following components in parts by mass: 50-200 parts of lavender, 100-200 parts of cornus officinalis fruits, 20-200 parts of zanthoxylum bungeanum fruits, and 20-100 parts of rhubarb root stalks.

2. The plant composition with weight loss function according to claim 1, characterized in that: The components specifically include: 200 parts of lavender, 200 parts of cornus officinalis fruits, 20 parts of Zanthoxylum bungeanum fruits and 20 parts of palmate rhubarb root stalks.

3. The plant composition with weight loss function according to claim 1, characterized in that: The components specifically include: 50 parts of lavender, 100 parts of cornus officinalis fruits, 200 parts of Zanthoxylum bungeanum fruits and 100 parts of palm leaf rhubarb root stalks.

4. A method for preparing a fermented extract of the plant composition according to claim 1, 2 or 3, characterized in that: The initial system is obtained by mixing the plant composition and fermentation bacteria, pure water 3 times the weight of the plant composition is added, and the fermented product obtained by fermentation is placed in a decoction machine, pure water 20 times the weight of the plant composition is added, extracted at 95-100°C for 1.5 hours, and filtered after impurity removal and purification.

5. The method for preparing the fermented extract of the plant composition according to claim 4, characterized in that: The fermentation bacteria is Saccharomyces cerevisiae. In the fermentation step, the temperature is 30-40°C, the stirring speed is 0-50rpm, and the time is 16-48h.

6. The method for preparing the fermented extract of the plant composition according to claim 4, characterized in that: The impurity removal and purification method is biological flocculation, and the flocculants used are water-soluble chitosan and carrageenan. After impurity removal and purification, the flavonoid components of the extract are enriched.

7. The method for preparing the fermented extract of the plant composition according to claim 4, characterized in that: The index contents in the fermentation extract include the following two items: total flavonoids ≥ 1.7 mg / mL and soluble solid content ≥ 4.8%.

8. The method for preparing the fermented extract of the plant composition according to claim 5, characterized in that: The index contents in the fermentation extract include the following two items: total flavonoids ≥ 1.7 mg / mL and soluble solid content ≥ 4.8%.

9. The method for preparing the fermented extract of the plant composition according to claim 6, characterized in that: The index contents in the fermentation extract include the following two items: total flavonoids ≥ 1.7 mg / mL and soluble solid content ≥ 4.8%.

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