Nano-composite gel for treating diabetic foot ulcer infectious wound and application of nano-composite gel

Abstract

The invention relates to the field of treatment of diabetic foot ulcer, and particularly discloses nano-composite gel for treating diabetic foot ulcer infectious wounds and application, the nano-composite gel is composed of a nano-material, metal ion liquid (Ionic Liquids), glucose oxidase (GOX) and arginine (L-Arg), the metal ion liquid is composed of dodecyl dimethyl benzyl ammonium chloride (DDBAC) and ferric trichloride (FeCl3), a main body of the nano material is prepared from hollow mesoporous silica nanoparticles (HMSN) and gel, GOX is fixed in the silica nanoparticles, L-Arg and the metal ion liquid are wrapped in the gel, and the metal ion liquid is formed by the hollow mesoporous silica nanoparticles and the gel. According to the scheme, the nano-composite gel has the functions of resisting infection, reducing blood glucose and promoting nerve angiogenesis, can effectively solve the problem that comprehensive diabetic foot ulcer infectious wounds are difficult to heal, can effectively treat diabetic foot ulcer, avoids ulcer recurrence, and improves the treatment efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of foot ulcer treatment, in particular to a nano composite gel for treating infected wounds of diabetic foot ulcers and an application thereof. Background Art

[0002] As the incidence of diabetes increases year by year, the incidence of diabetic complications also increases. Among them, the infection, amputation, and death associated with diabetic foot ulcers are receiving more and more attention. Diabetic foot ulcers are mainly manifested as chronic and refractory wounds. Due to the high-sugar microenvironment, which provides nutrition for bacteria, the wound is prone to bacterial biofilm infection, making it difficult for drugs to penetrate, causing drug resistance and affecting the effect of local treatment. It is also accompanied by damage to blood vessels and nerves. Therefore, diabetic foot ulcers recur repeatedly and are not healed, which not only troubles doctors' treatment, but also brings a serious burden to patients' physical and mental health.

[0003] Currently, the main topical drugs for the treatment of infected wounds of diabetic foot ulcers are erythromycin ointment, growth factor gel, etc., which have a single effect and often cannot solve the complex causes of infected wounds of diabetic foot ulcers (infection, high-sugar microenvironment, nerve damage, and vascular damage). Summary of the invention

[0004] The purpose of the present invention is to provide a nanocomposite gel for treating infected wounds of diabetic foot ulcers and its application, so as to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a nanocomposite gel for treating infected wounds of diabetic foot ulcers and its application.

[0006] A nanocomposite gel, which is composed of nanomaterials, metal ion liquids (Ionic Liquids), glucose oxidase (GOX) and arginine (L-Arg), the metal ion liquids (Ionic Liquids) are composed of dodecyl dimethyl benzyl ammonium chloride (DDBAC) and ferric chloride (FeCl3), the main body of the nanomaterial is made of hollow mesoporous silica nanoparticles (HMSN) + gel, GOX is fixed inside the silica nanoparticles, and L-Arg and the metal ion liquids are wrapped inside the gel.

[0007] A method for preparing a nanocomposite gel is applied to the nanocomposite gel as described above, wherein Carbomer 940 (200 mg) is added to 10 mL of pure water, placed in a 60° C. oil bath, and stirred to allow the Carbomer to fully swell;

[0008] Propylene glycol (100 mL) + 100 mg of low-viscosity sodium carboxycellulose was added, and then 200 mg of ethylparaben was fully dissolved in 3 mL of anhydrous ethanol. Then, 180 mg of triethanolamine was added under stirring to prepare a gel matrix.

[0009] Preferably, 10 mg of glucose oxidase (GOX) is dissolved in 1 mL of PBS, 10 mg of hollow mesoporous silica (HMSN) is added, the mixture is stirred for adsorption at room temperature for 8 hours, and centrifuged to obtain the hypoglycemic functional component.

[0010] Preferably, 68 mg of dodecyldimethylbenzylammonium chloride (DDBAC) is weighed into a 100 mL single-necked flask, 54 mg of FeCl3·6H2O is added, a magnetic bar and 10 mL of methanol are added, and the mixture is condensed and refluxed at 50° C. for 6 hours to obtain the antibacterial and nerve repair promoting functional component.

[0011] Preferably, 10 mg HMSN-GOX (content 12%) and 2.4 mg L-Arg are added to the gel matrix; the antibacterial and nerve repair promoting functional components (20 mg, content 0.1%) are added to the gel matrix and pure water, quantified to 20 g, and stirred evenly to obtain 20 g of nanocomposite gel per tube.

[0012] An external dressing made from any of the above-mentioned nanocomposite gels.

[0013] An application of an external dressing for treating infected wounds of diabetic foot ulcers, using the above-mentioned external dressing.

[0014] The present invention has at least the following beneficial effects:

[0015] 1. This nanocomposite gel has the functions of anti-infection, hypoglycemic and promoting nerve angiogenesis, and can effectively solve the problem of difficult healing of infected wounds of comprehensive diabetic foot ulcers;

[0016] 2. It is simple to make, inexpensive, practical and convenient, and has good antibacterial, hypoglycemic, and neuroangiogenesis-promoting effects. It can effectively treat diabetic foot ulcers, avoid ulcer recurrence, and improve treatment efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a graph showing the antibacterial effect (Staphylococcus aureus) of the antibacterial and nerve repair promoting functional components of the present invention;

[0018] Figure 2 This is the cytotoxicity diagram of the antibacterial and nerve repair promoting functional component of the present invention;

[0019] Figure 3 This is a cytotoxicity graph of the glucose-lowering functional component of the present invention;

[0020] Figure 4 This is a graph showing the antibacterial effect of the present invention on inhibiting Escherichia coli;

[0021] Figure 5 This is a graph showing the biocompatibility (hemolysis test) of the gel of the present invention;

[0022] Figure 6 It is the Fe ion release curve diagram of the present invention;

[0023] Figure 7 This is the GOX release curve of the present invention;

[0024] Figure 8 This is a scanning electron microscope (SEM) image of the gel of the present invention;

[0025] Fig. 9 This is a diagram of the cytotoxicity test of the present invention. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example

[0028] See also Figure 1-Figure 9 The present invention discloses a dressing for treating diabetic foot ulcers, which comprises nanomaterials, metal ion liquid (dodecyldimethylbenzylammonium chloride + ferric chloride), glucose oxidase (GOX) and arginine (L-Arg), and a hollow mesoporous structure of silica nanoparticles. GOX is fixed inside the silica nanoparticles, and L-Arg and the metal ion liquid are wrapped inside the gel.

[0029] The advantages are: simple preparation, low price, practical and convenient, good anti-infection, hypoglycemic, and neuroangiogenesis promoting effects, can effectively treat diabetic foot ulcers, avoid ulcer recurrence, and improve treatment efficiency;

[0030] GOX is fixed inside silica nanoparticles, and then L-Arg and DDBAC-FeCl3-IL are mixed into the gel together with silica nanoparticles. When the gel contacts glucose in diabetic ulcer wounds, GOX generates hydrogen peroxide (H2O2), and then L-Arg and H2O2 generate nitric oxide (NO), which promotes angiogenesis. The gel releases DDBAC-FeCl3-IL, which plays an anti-infection and nerve repair function.

[0031] Preparation of nanocomposite gel with anti-infection, hypoglycemic and neuroangiogenesis promoting functions, including the following preparation steps:

[0032] 1. Preparation of gel matrix: Carbomer 940 (200 mg) was added to 10 mL of pure water, placed in a 60°C oil bath, and stirred to fully swell the carbomer. Propylene glycol (100 mL) + 100 mg of low-viscosity sodium carboxycellulose was added, and then 200 mg of ethyl hydroxybenzoate was fully dissolved in 3 mL of anhydrous ethanol. Under stirring, 180 mg of triethanolamine was added to prepare a gel matrix.

[0033] 2. Preparation of hypoglycemic functional ingredients: Dissolve 10 mg of glucose oxidase (GOX) in 1 mL of pure water, add 10 mg of hollow mesoporous silica (HMSN), adsorb with stirring at room temperature for 8 hours, and centrifuge.

[0034] 3. Preparation of antibacterial and nerve repair promoting functional components: Weigh 68 mg of dodecyldimethylbenzyl ammonium chloride (DDBAC) into a 100 mL single-necked flask, add 54 mg of FeCl3·6H2O, a magnetic bar, and 10 mL of methanol, and reflux at 50°C for 6 hours.

[0035] 4. Preparation of finished product: Add 10 mg HMSN-GOX (content 12%) and 2.4 mg L-Arg to the gel matrix; add the antibacterial and nerve repair promoting functional components (20 mg, content 0.1%) to the gel matrix and pure water, quantify to 20 g, stir evenly, and obtain 20 g of diabetic foot nanocomposite gel per tube.

[0036] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0037] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A nanocomposite gel, characterized in that: The nanocomposite gel is composed of nanomaterials, metal ion liquids (Ionic Liquids), glucose oxidase (GOX) and arginine (L-Arg). The metal ion liquids (Ionic Liquids) are composed of dodecyldimethylbenzylammonium chloride (DDBAC) and ferric chloride (FeCl3). The main body of the nanomaterial is made of hollow mesoporous silica nanoparticles (HMSN) + gel. GOX is fixed inside the silica nanoparticles, and L-Arg and metal ion liquids are wrapped inside the gel.

2. A method for preparing a nanocomposite gel, applied to the nanocomposite gel as claimed in claim 1, characterized in that: Add Carbomer 940 (200 mg) to 10 mL of pure water, place in a 60°C oil bath, and stir to allow the Carbomer to fully swell; Propylene glycol (100 mL) + 100 mg of low-viscosity sodium carboxycellulose was added, and then 200 mg of ethylparaben was fully dissolved in 3 mL of anhydrous ethanol. Then, 180 mg of triethanolamine was added under stirring to prepare a gel matrix.

3. The method for preparing the nanocomposite gel according to claim 2, characterized in that: 10 mg of glucose oxidase (GOX) was dissolved in 1 mL of pure water, and 10 mg of hollow mesoporous silica (HMSN) was added. The mixture was stirred and adsorbed at room temperature for 8 hours, and then centrifuged to obtain the hypoglycemic functional component.

4. The method for preparing the nanocomposite gel according to claim 3, characterized in that: Weigh 68 mg of dodecyldimethylbenzylammonium chloride (DDBAC) into a 100 mL single-necked flask, add 54 mg of FeCl3·6H2O, add a magnetic bar, and 10 mL of methanol, and react at 50°C for 6 hours to obtain an antibacterial and nerve repair promoting component.

5. The method for preparing the nanocomposite gel according to claim 4, characterized in that: 10 mg HMSN-GOX (content 12%) and 2.4 mg L-Arg were added to the gel matrix; the antibacterial and nerve repair promoting functional components (20 mg, content 0.1%) were added to the gel matrix and pure water, quantified to 20 g, and stirred evenly to obtain 20 g of nanocomposite gel per tube.

6. An external dressing made from the nanocomposite gel according to any one of claims 1 to 5.

7. Use of an external dressing for treating infected wounds of diabetic foot ulcers, characterized in that: Use the external dressing as claimed in claim 6.

Images