Equipment and method for producing double emulsion drops with trehalose

Abstract

The invention discloses equipment and a method for producing double-emulsion drops with trehalose, and belongs to the technical field of preparation of double-emulsion drops. Equipment for producing double emulsion drops with trehalose comprises a sterile box with a top cover and further comprises a dissolving barrel rotationally arranged in the sterile box, a filter membrane of an annular structure is detachably arranged at the top of the dissolving barrel, a collecting barrel is fixedly arranged in the sterile box and communicated with the output end of the filter membrane, and the bottom in the collecting barrel is obliquely arranged; the top of the dissolving barrel is provided with the filter membrane of an annular structure, after a solution in the dissolving barrel is uniformly mixed, the lifting plate lifts the solution in the dissolving barrel, then the solution flows towards the filter membrane under the action of centrifugal force, after the solution is filtered by the filter membrane, the collecting barrel can collect the filtered solution, and in the process, the solution can be uniformly dissolved in the dissolving barrel. Seamless connection of dissolving and filtering of the trehalose can be realized, the intermediate transfer process is reduced, the risk that the trehalose emulsion is polluted is reduced, and the use experience is improved.

Description

Technical Field

[0001] The invention relates to the technical field of double emulsion droplet preparation, and in particular to a device and method for producing double emulsion droplets containing trehalose. Background Art

[0002] An emulsion is a mixture of two or more immiscible liquids, in which one liquid (dispersed phase) is distributed in the form of droplets in another liquid (continuous phase). Furthermore, a double emulsion (also known as a double emulsion) is an emulsion in an emulsion or an encapsulated droplet, that is, a highly structured fluid in which smaller droplets (sub-droplets) are encapsulated in droplets of a dispersed liquid. Typical types are water-in-oil-in-water (W / O / W) and oil-in-water-in-oil (O / W / O). Among them, double emulsion droplets containing trehalose are the continuous phase because trehalose is a natural disaccharide that can form a glassy structure under extreme conditions (such as drying, freezing or high temperature), encapsulate and protect the internal biological molecules (such as proteins, enzymes, cells, etc.) to prevent them from denaturing or inactivating. Not only can the properties of trehalose itself be used to protect and stabilize the internal components, but also the needs of different industries for high-performance and multifunctional materials can be met.

[0003] At present, the manufacture of double emulsion droplets generally adopts droplet microfluidics technology. This technology uses microfluidic chips with different structures to control the convection of immiscible liquids, form microdroplets under the action of shear and extrusion, and assemble the microdroplet spherical structure in two or more steps. It has preliminarily realized the controllable design and manipulation of the size, morphology and function of double emulsion droplets.

[0004] When processing trehalose emulsion, it is necessary to use a suitable solvent (the most common one is deionized water or a specific buffer) to stir with trehalose powder to dissolve it, and then select a filter membrane with an appropriate pore size to filter the dissolved solution; however, currently, two devices are usually used to dissolve and filter trehalose. Since trehalose emulsion needs to be processed in a sterile environment, the use of two devices not only increases the risk of contamination of the trehalose emulsion, but also places high requirements on the transfer device during the transfer process, affecting the use effect. Summary of the invention

[0005] The purpose of the present invention is to solve the problems of separate processing in the prior art, which increases the risk of trehalose emulsion being contaminated during the transfer process, and the high requirements for the transfer device, and to propose a device and method for producing double emulsion droplets with trehalose.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A device for producing double emulsion droplets containing trehalose comprises a sterile box with a top cover, and also comprises a dissolving cylinder rotatably arranged in the sterile box, a filter membrane with an annular structure is detachably arranged on the top of the dissolving cylinder, and a collecting cylinder is fixedly arranged in the sterile box, the collecting cylinder is connected to the output end of the filter membrane, and the bottom of the collecting cylinder is inclined.

[0008] In order to facilitate the filter membrane to filter the solution in the dissolution cylinder, preferably, a lifting plate for lifting the solution is arranged in the dissolution cylinder, a sealing ring is fixedly arranged between the outer wall of the lifting plate and the inner wall of the dissolution cylinder, and a blade is fixedly arranged on the top end face of the lifting plate.

[0009] In order to facilitate the driving of the lifting plate to rise and fall, the sterile box is further fixedly provided with a cylinder, the output end of the cylinder extends to one end inside the dissolving cylinder and is fixedly connected to the bottom of the lifting plate, and a plurality of groups of telescopic rods are fixedly provided in the sterile box, and the telescopic ends of the telescopic rods are fixedly connected to the bottom of the lifting plate.

[0010] In order to clean the inner wall of the filter membrane during the filtration process, a scraper for cleaning the inner wall of the filter membrane is further fixedly provided on the top of the lifting plate. When the lifting plate rises, the outer wall of the scraper abuts against the inner wall of the filter membrane.

[0011] Preferably, a fixing ring is fixedly provided on the top of the dissolving cylinder via a supporting rod, a fixing cylinder is threadedly connected to the fixing ring, and the filter membrane is fixedly provided on the inner wall of the fixing cylinder.

[0012] Furthermore, an outer baffle cover is fixedly provided on the outer wall of the fixed cylinder, an inner baffle cover corresponding to the outer baffle cover is fixedly provided on the outer wall of the top of the dissolving cylinder, an annular groove is opened on the top of the collecting cylinder, a feed channel is formed between the outer baffle cover and the inner baffle cover, the output end of the filter membrane is connected with the top of the feed channel, the bottom of the feed channel is connected with the annular groove, and a discharge pipe is fixedly provided on the collecting cylinder.

[0013] Furthermore, a wind blade is fixedly provided on the outer wall of one end of the outer shield extending into the collecting tube, and a through hole is opened on the top of the collecting tube. When the wind blade rotates, the air in the sterile box enters the collecting tube through the through hole.

[0014] Furthermore, a heating chamber is provided in the dissolving cylinder, a heating wire is fixedly provided in the heating chamber, and an inert gas is provided in the heating chamber.

[0015] Furthermore, the outer wall of one end of the inner baffle extending into the collecting tube is provided with a groove body, an elastic diaphragm is fixedly provided on the end face of the groove body mouth, a protrusion is fixedly provided on the inner wall of the collecting tube, the protrusion cooperates with the elastic diaphragm, and the groove body is connected with the heating chamber through a pipe.

[0016] A method for producing double emulsion droplets with trehalose, further comprising the following steps:

[0017] Step 1: First, sterilize the sterile box, then open the top cover and put a certain amount of solvent and trehalose powder into the dissolving cylinder, drive the dissolving cylinder to rotate for dissolution, and set the heating temperature of the heating wire for the solution;

[0018] Step 2: After sufficient dissolution, the lifting plate rises to push the solution in the dissolution cylinder to the filter membrane for centrifugal filtration;

[0019] Step 3: The filtered solution enters the collecting cylinder through the discharge channel and is finally discharged through the discharge pipe.

[0020] Compared with the prior art, the present invention provides a device for producing double emulsion droplets containing trehalose, which has the following beneficial effects:

[0021] 1. The double emulsion droplet equipment for producing trehalose has a ring-shaped filter membrane on the top of the dissolving cylinder. After the solution in the dissolving cylinder is evenly mixed, the lifting plate lifts the solution in the dissolving cylinder, and then the solution flows to the filter membrane under the action of centrifugal force. After being filtered by the filter membrane, the collecting cylinder can collect the filtered solution. In this process, the dissolution and filtration of trehalose can be seamlessly connected, the intermediate transfer process can be reduced, the risk of trehalose emulsion being contaminated can be reduced, and the use experience can be improved;

[0022] 2. The equipment for producing double emulsion droplets containing trehalose has a lifting plate in the dissolving cylinder. When dissolving and stirring, the lifting plate is located at the inner bottom of the dissolving cylinder. At this time, the solution is also at the bottom of the dissolving cylinder. The dissolving cylinder can rotate to stir and mix the solution, thereby reducing the possibility of splashing the solution and improving the use effect.

[0023] 3. The double emulsion droplet equipment for producing trehalose can extend the scraper from the dissolving cylinder and move toward the filter membrane while the lifting plate is carrying the solution to rise. At this time, the dissolving cylinder rotates with the filter membrane to filter. The scraper can clean the material trapped on the inner wall of the filter membrane to improve the filtering efficiency of the filter membrane. The lifting plate can be driven to carry the scraper to rise and fall reciprocally, which can not only increase the effect on the solution, but also improve the cleaning quality of the inner wall of the filter membrane.

[0024] 4. The equipment for producing double emulsion droplets containing trehalose, through the rotation of the dissolving cylinder with the outer baffle and the wind blades, the air in the sterile box can enter the collecting cylinder through the through hole. On the one hand, when the discharge pipe is opened for discharge, the airflow entering the collecting cylinder can have a certain pushing effect on the material, which is convenient for discharge. On the other hand, when the airflow flows into the collecting cylinder, a part of the gas will pass through the filter membrane and enter the collecting cylinder along the discharge channel, which can not only increase the speed of the solution sliding downward on the inner wall of the discharge channel and reduce the aggregation in the discharge channel, but also have a certain dredging effect on the filter membrane, thereby accelerating the filtration of the solution;

[0025] 5. The double emulsion droplet equipment for producing trehalose, when the dissolving cylinder rotates with the inner baffle and the elastic diaphragm, when the elastic diaphragm passes through the convex block, the elastic diaphragm will be squeezed by the convex block, and the elastic diaphragm will be pushed into the tank body. After the elastic diaphragm slides over the convex block, the elastic diaphragm returns to its original state under its own elastic action. At this time, the elastic diaphragm continuously squeezes the gas in the tank body, which can not only make the gas in the heating chamber shake or even flow, thereby increasing the heating effect in the dissolving cylinder, but also the elastic deformation of the elastic diaphragm can cause the inner baffle to produce a certain vibration effect, thereby improving the flow of materials in the feeding channel.

[0026] The parts not involved in the device are the same as the prior art or can be implemented by using the prior art. The present invention arranges a ring-shaped filter membrane on the top of the dissolution cylinder. After the solution in the dissolution cylinder is evenly mixed, the lifting plate lifts the solution in the dissolution cylinder, and then it flows to the filter membrane under the action of centrifugal force. After being filtered by the filter membrane, the collection cylinder can collect the filtered solution. In this process, the dissolution and filtration of trehalose can be seamlessly connected, the intermediate transfer process can be reduced, the risk of contamination of trehalose emulsion can be reduced, and the user experience can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the structure of a device for producing double emulsion droplets containing trehalose proposed by the present invention;

[0028] Figure 2 A cross-sectional view of a device for producing double emulsion droplets containing trehalose proposed by the present invention;

[0029] Figure 3 A double emulsion droplet device for producing trehalose is proposed by the present invention Figure 2 A magnified view of part A;

[0030] Figure 4 This is a schematic diagram of the structure of a collection tube for producing double emulsion droplets containing trehalose proposed by the present invention;

[0031] Figure 5An exploded view of a dissolving cylinder of a double emulsion droplet device for producing trehalose provided by the present invention;

[0032] Figure 6 A partial cross-sectional view of a dissolving cylinder of a double emulsion droplet device for producing trehalose provided by the present invention.

[0033] In the figure: 1. Sterile box; 101. Top cover; 2. Dissolving cylinder; 201. Outer baffle; 202. Inner baffle; 203. Heating chamber; 204. Support rod; 205. Fixing ring; 206. Pipe; 207. Tank body; 208. Elastic diaphragm; 3. Fixing cylinder; 301. Filter membrane; 4. Collecting cylinder; 401. Discharge pipe; 402. Through hole; 403. Annular groove; 404. Bump; 5. Wind blade; 6. Lifting plate; 601. Scraper; 602. Blade; 603. Cylinder; 604. Telescopic rod. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0036] Example:

[0037] Reference Figure 1-Figure 6A device and method for producing double emulsion droplets containing trehalose, comprising a sterile box 1 with a top cover 101, wherein a sterilizing lamp is fixedly arranged on the inner wall of the top cover 101. Before and after use, the sterilizing lamp can be turned on to sterilize the sterile box 1 to reduce bacteria in the sterile box 1 and improve the use effect. The invention also includes a dissolving cylinder 2 rotatably arranged in the sterile box 1, a rotating cylinder is fixedly arranged at the bottom of the dissolving cylinder 2, and the rotating cylinder is rotatably connected to the sterile box 1, a driving box is fixedly arranged in the sterile box 1, a first gear is fixedly arranged on the outer wall of one end of the rotating cylinder extending into the driving box, a motor is fixedly arranged in the driving box, and a second gear meshing with the first gear is fixedly arranged at the output end of the motor. When in use, the second gear is driven to rotate by starting the motor, and the second gear is meshed with the first gear to drive the rotating cylinder and the dissolving cylinder 2 to rotate, so as to achieve stirring and dissolving of the trehalose and the solvent in the dissolving cylinder 2, a filter membrane 301 with an annular structure is disassembled and arranged on the top of the dissolving cylinder 2, and a collecting cylinder 4 is fixedly arranged in the sterile box 1, and the collecting cylinder 4 rotates approximately The body is truncated cone-shaped and is sleeved on the outer wall of the dissolving cylinder 2, but the bottom of the collecting cylinder 4 is inclined, which is not only convenient for collecting the filtered solution, but also can make the solution gather to the discharge pipe 401 to improve the discharge effect, and the collecting cylinder 4 is connected with the output end of the filter membrane 301. When in use, a ring-shaped filter membrane 301 is arranged on the top of the dissolving cylinder 2. After the solution in the dissolving cylinder 2 is evenly mixed, the lifting plate 6 lifts the solution in the dissolving cylinder 2, and then flows to the filter membrane 301 under the action of centrifugal force. After being filtered by the filter membrane 301, the collecting cylinder 4 can collect the filtered solution. In this process, the dissolution and filtration of trehalose can be seamlessly connected, the intermediate transfer process can be reduced, the risk of contamination of trehalose emulsion can be reduced, and the use experience can be improved.

[0038] Reference Figure 2 A lifting plate 6 for lifting the solution is set in the dissolving cylinder 2 for lifting, and a sealing ring is fixedly arranged between the outer wall of the lifting plate 6 and the inner wall of the dissolving cylinder 2. When in use, the sealing effect between the outer wall of the lifting plate 6 and the inner wall of the dissolving cylinder 2 can be improved, and the solution can be reduced from entering the bottom of the lifting plate 6 through the connection between the lifting plate 6 and the dissolving cylinder 2, thereby improving the use effect, and a blade 602 is fixedly arranged on the top end surface of the lifting plate 6. When in use, the lifting plate 6 is set in the dissolving cylinder 2 for lifting. When dissolving and stirring, the lifting plate 6 is located at the inner bottom of the dissolving cylinder 2. At this time, the solution is also at the bottom of the dissolving cylinder 2. The dissolving cylinder 2 can be rotated to stir and mix the solution, thereby reducing the possibility of splashing of the solution and improving the use effect.

[0039] Reference Figure 2A cylinder 603 is fixedly installed in the sterile box 1, and the output end of the cylinder 603 is extended to one end in the dissolving cylinder 2 and fixedly connected to the bottom of the lifting plate 6, and a plurality of groups of telescopic rods 604 are fixedly installed in the sterile box 1. The telescopic rods 604 are provided with two to six groups, preferably two groups. The telescopic ends of the two groups of telescopic rods 604 are fixedly connected to the bottom of the lifting plate 6. When in use, the lifting plate 6 is raised in the dissolving cylinder 2 by starting the cylinder 603 so that it rises a certain distance from the bottom of the filter membrane 301. The dissolved solution in the dissolving cylinder 2 can be lifted, which is convenient for filtering through the filter membrane 301 and improving the use effect.

[0040] Reference Figure 2 A scraper 601 for cleaning the inner wall of the filter membrane 301 is fixedly provided on the top of the lifting plate 6. When the lifting plate 6 rises, the outer wall of the scraper 601 is against the inner wall of the filter membrane 301. When in use, when the lifting plate 6 is used to carry the solution up, the scraper 601 can be extended from the dissolution cylinder 2 and move toward the filter membrane 301. At this time, the dissolution cylinder 2 rotates with the filter membrane 301 for filtering. The scraper 601 can clean the material trapped on the inner wall of the filter membrane 301, thereby improving the filtering efficiency of the filter membrane 301. The lifting plate 6 can be driven to carry the scraper 601 to rise and fall reciprocally, which can not only increase the effect on the solution, but also improve the cleaning quality of the inner wall of the filter membrane 301.

[0041] Reference Figure 2 , Figure 5 and Figure 6 A fixing ring 205 is fixedly provided at the top of the dissolving cylinder 2 through a supporting rod 204, and a fixing cylinder 3 is threadedly connected to the fixing ring 205, and the filter membrane 301 is fixedly provided on the inner wall of the fixing cylinder 3. When in use, the filter membrane 301 is fixed to the fixing cylinder 3, and then the fixing cylinder 3 is threadedly disassembled and connected to the fixing ring 205, which facilitates the installation and disassembly of the filter membrane 301 and improves the efficiency of maintenance and replacement of the filter membrane 301.

[0042] Reference Figure 2-Figure 6, an outer baffle 201 is fixedly arranged on the outer wall of the fixed cylinder 3, and an inner baffle 202 corresponding to the outer baffle 201 is fixedly arranged on the top outer wall of the dissolving cylinder 2, and both the outer baffle 201 and the inner baffle 202 are trumpet-shaped structures, and an annular groove 403 is opened at the top of the collecting cylinder 4. At this time, a feeding channel is surrounded by the outer baffle 201 and the inner baffle 202, and the output end of the filter membrane 301 is connected with the top of the feeding channel, and the bottom of the feeding channel is connected with the annular groove 403. A discharge pipe 401 is fixedly arranged on the collecting cylinder 4, and a valve is fixedly arranged on one end of the discharge pipe 401 extending out of the sterile box 1. When in use, by fixing the outer baffle 201 and the inner baffle 202 on the dissolving cylinder 2, the solution filtered by the filter membrane 301 can be collected, and splashing into and out of the sterile box 1 can be reduced. At this time, the solution can enter the collecting cylinder 4 through the feeding channel, which is convenient for subsequent use.

[0043] Reference Figure 2-Figure 6 , a wind blade 5 is fixedly arranged on the outer wall of one end of the outer baffle 201 extending into the collecting cylinder 4, and the wind blade 5 is evenly arranged in multiple groups, preferably fifty groups, and a through hole 402 is opened at the top of the collecting cylinder 4. When the dissolving cylinder 2 rotates with the outer baffle 201 and the wind blade 5, the air in the sterile box 1 can enter the collecting cylinder 4 through the through hole 402. On the one hand, when the discharge pipe 401 is opened for discharge, the airflow entering the collecting cylinder 4 can have a certain pushing effect on the material, which is convenient for discharge. On the other hand, when the airflow flows into the collecting cylinder 4, a part of the gas will pass through the filter membrane 301 and enter the collecting cylinder 4 along the discharge channel, which can not only increase the speed at which the solution on the inner wall of the discharge channel slides downward and reduce the aggregation in the discharge channel, but also have a certain dredging effect on the filter membrane 301, thereby accelerating the filtration of the solution.

[0044] Reference Figure 2 A heating chamber 203 is provided in the dissolving cylinder 2, and a heating wire is fixedly arranged in the heating chamber 203. Here, since the dissolving cylinder 2 rotates, the power supply method of the heating wire refers to the power supply connection of the motor coil, and an inert gas is arranged in the heating chamber 203. When in use, by providing a heating chamber 203 in the dissolving cylinder 2, a heating wire for heating the solution in the dissolving cylinder 2 is fixedly arranged in the heating chamber 203. When in use, the solution can be appropriately heated, and the temperature generally does not exceed 60°C, which helps to accelerate the mixing effect of the solution. Moreover, the heating chamber 203 is filled with inert gas, which can realize heating the gas, and then the temperature is transferred to the inner wall of the dissolving cylinder 2 through the gas, thereby improving the use effect.

[0045] Reference Figure 2 and Figure 3A groove 207 is provided on the outer wall of one end of the inner shield 202 extending into the collecting cylinder 4, an elastic diaphragm 208 is fixedly provided on the mouth end face of the groove 207, a convex block 404 is fixedly provided on the inner wall of the collecting cylinder 4, and both ends of the convex block 404 smoothly transition with the inner wall of the collecting cylinder 4, and the cross section of the elastic diaphragm 208 is a thin-walled hemispherical structure, the convex block 404 is matched with the elastic diaphragm 208, and the groove 207 is connected with the heating chamber 203 through the pipeline 206. When in use, when the dissolving cylinder 2 is rotated with the inner shield 202 and the elastic diaphragm 208, when the elastic diaphragm 208 passes through the convex block When the block 404 is pressed, the elastic diaphragm 208 is squeezed by the protrusion 404, pushing the elastic diaphragm 208 into the groove body 207. After the elastic diaphragm 208 slides over the protrusion 404, the elastic diaphragm 208 returns to its original state under the action of its own elasticity. At this time, the elastic diaphragm 208 continuously squeezes the gas in the groove body 207, which can not only make the gas in the heating chamber 203 shake or even flow, thereby increasing the heating effect in the dissolving cylinder 2, but also the elastic deformation of the elastic diaphragm 208 can cause the inner baffle 202 to produce a certain vibration effect, thereby improving the flow of materials in the discharge channel.

[0046] A method for producing double emulsion droplets with trehalose,

[0047] Step 1: First, sterilize the sterile box 1, then open the top cover 101 and put a certain amount of solvent and trehalose powder into the dissolving cylinder 2, drive the dissolving cylinder 2 to rotate for dissolution, and set the heating temperature of the heating wire for the solution (the heating temperature does not exceed 60° C.);

[0048] When in use, first turn on the sterilization lamp to sterilize the sterile box 1, then open the top cover 101, add a suitable and quantitative solvent and trehalose powder into the dissolving cylinder 2, close the top cover 101, then turn on the sterilization lamp again to perform the sterilization operation, after the sterilization is completed, drive the dissolving cylinder 2 to rotate to stir and mix the solvent and trehalose powder, and at this time, the dissolving cylinder 2 can be properly heated by the heating wire to help speed up the dissolution speed;

[0049] Step 2: After sufficient dissolution, the lifting plate 6 rises to push the solution in the dissolution cylinder 2 to the filter membrane 301 for centrifugal filtration;

[0050] After the dissolution is completed, the cylinder 603 drives the lifting plate 6 to carry the solution in the dissolution cylinder 2 up to the filter membrane 301. The dissolution cylinder 2 rotates to throw the solution to the filter membrane 301 for filtration, which can reduce the transfer steps and improve the use effect.

[0051] Step 3: The filtered solution enters the collecting tube 4 through the discharge channel and is finally discharged through the discharge pipe 401;

[0052] The collecting cylinder 4 can collect the filtered solution, and then open the discharge pipe 401 to discharge it, thereby improving the use effect.

[0053] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for producing double emulsion droplets containing trehalose, comprising a sterile box (1) with a top cover (101), characterized in that: It also includes a dissolving cylinder (2) rotatably arranged in the sterile box (1), the top of the dissolving cylinder (2) being provided with a filter membrane (301) in an annular structure, and a collecting cylinder (4) being fixedly arranged in the sterile box (1), the collecting cylinder (4) being connected to the output end of the filter membrane (301), and the bottom of the collecting cylinder (4) being arranged in an inclined manner.

2. The device for producing double emulsion droplets containing trehalose according to claim 1, characterized in that: A lifting plate (6) for lifting the solution is arranged in the dissolving cylinder (2), a sealing ring is fixedly arranged between the outer wall of the lifting plate (6) and the inner wall of the dissolving cylinder (2), and a blade (602) is fixedly arranged on the top end surface of the lifting plate (6).

3. The device for producing double emulsion droplets containing trehalose according to claim 2, characterized in that: The sterile box (1) is fixedly provided with a cylinder (603), and one end of the cylinder (603) extends into the dissolving cylinder (2) and is fixedly connected to the bottom of the lifting plate (6). In addition, a plurality of groups of telescopic rods (604) are fixedly provided in the sterile box (1), and the telescopic ends of the telescopic rods (604) are fixedly connected to the bottom of the lifting plate (6).

4. The device for producing double emulsion droplets containing trehalose according to claim 3, characterized in that: A scraper (601) for cleaning the inner wall of the filter membrane (301) is fixedly arranged on the top of the lifting plate (6); when the lifting plate (6) rises, the outer wall of the scraper (601) abuts against the inner wall of the filter membrane (301).

5. The device for producing double emulsion droplets containing trehalose according to claim 1, characterized in that: A fixing ring (205) is fixedly arranged on the top of the dissolving cylinder (2) via a supporting rod (204), a fixing cylinder (3) is threadedly connected to the fixing ring (205), and the filter membrane (301) is fixedly arranged on the inner wall of the fixing cylinder (3).

6. The device for producing double emulsion droplets containing trehalose according to claim 5, characterized in that: An outer baffle (201) is fixedly provided on the outer wall of the fixed cylinder (3); an inner baffle (202) corresponding to the outer baffle (201) is fixedly provided on the outer wall of the top of the dissolving cylinder (2); an annular groove (403) is provided on the top of the collecting cylinder (4); a material discharge channel is formed between the outer baffle (201) and the inner baffle (202); the output end of the filter membrane (301) is connected to the top of the material discharge channel; the bottom of the material discharge channel is connected to the inside of the annular groove (403); and a discharge pipe (401) is fixedly provided on the collecting cylinder (4).

7. The device for producing double emulsion droplets containing trehalose according to claim 6, characterized in that: A wind blade (5) is fixedly provided on the outer wall of one end of the outer shield (201) extending into the collecting tube (4), and a through hole (402) is opened at the top of the collecting tube (4). When the wind blade (5) rotates, the air in the sterile box (1) enters the collecting tube (4) through the through hole (402).

8. The device for producing double emulsion droplets containing trehalose according to claim 7, characterized in that: A heating chamber (203) is provided in the dissolving cylinder (2), a heating wire is fixedly arranged in the heating chamber (203), and an inert gas is provided in the heating chamber (203).

9. The device for producing double emulsion droplets containing trehalose according to claim 8, characterized in that: The outer wall of one end of the inner shield (202) extending into the collecting tube (4) is provided with a groove (207), an elastic diaphragm (208) is fixedly provided on the mouth end face of the groove (207), a protrusion (404) is fixedly provided on the inner wall of the collecting tube (4), the protrusion (404) cooperates with the elastic diaphragm (208), and the groove (207) is connected to the heating chamber (203) through a pipe (206).

10. A method for producing double emulsion droplets with trehalose, comprising the device for producing double emulsion droplets with trehalose according to any one of claims 1 to 9, characterized in that: The following steps are also included: Step 1: firstly, the sterile box (1) is sterilized, then the top cover (101) is opened, a certain amount of solvent and trehalose powder are placed into the dissolving cylinder (2), the dissolving cylinder (2) is driven to rotate for dissolution, and the heating temperature of the heating wire for the solution is set; Step 2: After sufficient dissolution, the lifting plate (6) rises to push the solution in the dissolution cylinder (2) to the filter membrane (301) for centrifugal filtration; Step 3: The filtered solution enters the collecting cylinder (4) through the discharge channel and is finally discharged through the discharge pipe (401).

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