A method for preparing trace elements in yeast nucleotide chelate for agricultural use

Abstract

The present invention relates to a method for preparing trace elements in yeast nucleotide chelate for agricultural use, characterized in that the method comprises the following steps: dissolving yeast nucleotide and trace element oxides or salts in water, reacting for 20 to 100 minutes at a pH of 3.0 to 3.5 and a temperature of 20 to 80° C., and obtaining the trace elements in yeast nucleotide chelate for agricultural use. The trace elements in yeast nucleotide chelate for agricultural use prepared in the present application have a high yield, good compatibility with fertilizers, and good effect when applied to agriculture.

Description

Technical Field

[0001] The invention relates to a method for preparing trace elements in yeast nucleotide chelation for agricultural use. Background Art

[0002] Yeast nucleotides contain nucleic acids, nucleotides, polypeptides, flavoring agents and polysaccharides, etc., and are currently mainly used in feed to improve animal immunity, promote growth and development, and digestion and absorption. The amount added to each ton of feed is 0.1-1.0 kg. Currently, it is rarely used in agriculture.

[0003] The application of trace elements in plants mainly plays a role in promoting nutrient balance, improving plant resistance, and improving the quality of agricultural products. Currently, they are divided into inorganic and chelated states. The chelated state has better fertilizer compatibility and high utilization rate and is loved by farmers. EDTA chelation is the main chelated trace element in the market.

[0004] Yeast nucleotides contain a large amount of nucleic acids, nucleotides, polypeptides, flavoring agents and polysaccharides, that is, they contain phosphate groups, nitrogenous bases, carboxyl groups and hydroxyl groups, and have the potential to chelate trace elements. However, due to the current low application of yeast nucleotides in agriculture, there are fewer studies on trace elements in yeast nucleotide chelation, especially the trace elements in yeast nucleotide chelation used in agriculture.

[0005] At present, there are no products and methods for chelating trace elements using yeast nucleotides, nor are there products and methods for applying yeast nucleotide chelated trace elements to agriculture. Summary of the invention

[0006] The invention provides a method for preparing trace elements chelated by yeast nucleotide for agriculture, and solves the technical problems of 1) chelating trace elements by yeast nucleotide; and 2) applying the trace elements chelated by yeast nucleotide to agriculture.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0008] A method for preparing yeast nucleotide chelated trace elements for agricultural use, comprising the steps of adding yeast nucleotide and trace element oxides or salts into water, reacting for 20 to 100 minutes at a pH of 3.0 to 3.5 and a temperature of 20 to 80° C., to obtain liquid yeast nucleotide chelated trace elements for agricultural use;

[0009] The liquid agricultural yeast nucleotide chelated trace elements are dried to obtain the powdered agricultural yeast nucleotide chelated trace elements.

[0010] The trace elements are iron, zinc, calcium and magnesium; the salt is one or more of sulfate, nitrate and chloride.

[0011] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, and is extracted from Candida.

[0012] The mass ratio of yeast nucleotide, trace element oxide or salt and water is 15-30:1-20:50-84.

[0013] Also included is phosphatidylethanolamine-polyethylene glycol 2000.

[0014] According to the following steps, yeast nucleotides and trace element oxides or salts are dissolved in water, and reacted at a pH of 3.0 to 3.5 and a temperature of 20 to 80°C for 20 to 100 minutes to obtain product A:

[0015] Add phosphatidylethanolamine-polyethylene glycol 2000 into water and dissolve it at a temperature of 50-80°C to obtain product B;

[0016] Mix product A and product B to obtain liquid agricultural yeast nucleotide chelated trace elements;

[0017] The liquid agricultural yeast nucleotide chelated trace elements are dried to obtain the powdered agricultural yeast nucleotide chelated trace elements.

[0018] The mass ratio of yeast nucleotides, trace element oxides or salts and water is 15-30:1-20:50-84;

[0019] The mass ratio of phosphatidylethanolamine-polyethylene glycol 2000 and water is 5-20:80-95;

[0020] The mass ratio of product A to product B is 90-98:2-10.

[0021] The invention has the following beneficial technical effects:

[0022] 1) The agricultural yeast nucleotide chelate prepared in the present application has a high yield of trace elements.

[0023] 2) The trace elements in the agricultural yeast nucleotide chelate prepared in this application have good effects in agriculture. DETAILED DESCRIPTION

[0024] In order to better understand the above technical solution, the technical solution of the present invention is described in detail below in conjunction with preferred examples.

[0025] Example 1

[0026] A method for preparing yeast nucleotide chelated magnesium for agricultural use is carried out according to the following steps: adding yeast nucleotide and magnesium oxide into water, reacting for 60 minutes at a pH of 3.0 and a temperature of 70° C., and obtaining a filtrate to obtain liquid yeast nucleotide chelated magnesium for agricultural use;

[0027] The liquid agricultural yeast nucleotide chelated magnesium is dried to obtain the powdered agricultural yeast nucleotide chelated magnesium.

[0028] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0029] The mass ratio of yeast nucleotides, magnesium oxide and water is 20:2:78.

[0030] The magnesium detection adopts EDTA titration method to determine the yield of chelated powder agricultural yeast nucleotide chelated magnesium. The conversion rate of magnesium into yeast nucleotide chelated magnesium is calculated to be 72.33%.

[0031] Example 2

[0032] A method for preparing yeast nucleotide chelated magnesium for agricultural use is carried out according to the following steps: adding yeast nucleotide and magnesium oxide into water, reacting for 60 minutes at a pH of 3.5 and a temperature of 70° C., and obtaining a filtrate to obtain liquid yeast nucleotide chelated magnesium for agricultural use;

[0033] The liquid agricultural yeast nucleotide chelated magnesium is dried to obtain the powdered agricultural yeast nucleotide chelated magnesium.

[0034] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0035] The mass ratio of yeast nucleotides, magnesium oxide and water is 20:2:78.

[0036] The magnesium was detected by EDTA titration method to determine the yield of the chelated powder agricultural yeast nucleotide chelated magnesium. The conversion rate of magnesium into yeast nucleotide chelated magnesium was calculated to be 72.16%.

[0037] Example 3

[0038] A method for preparing yeast nucleotide chelated magnesium for agricultural use, comprising the steps of adding yeast nucleotide and magnesium sulfate pentahydrate into water, reacting for 60 minutes at a pH of 3.0 and a temperature of 70° C., to obtain liquid yeast nucleotide chelated magnesium for agricultural use;

[0039] The liquid agricultural yeast nucleotide chelated magnesium is dried to obtain the powdered agricultural yeast nucleotide chelated magnesium.

[0040] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0041] The mass ratio of yeast nucleotide, magnesium sulfate pentahydrate and water is 20:10:71.4.

[0042] The magnesium was detected by EDTA titration method to determine the yield of the chelated powder agricultural yeast nucleotide chelated magnesium. The conversion rate of magnesium into yeast nucleotide chelated magnesium was calculated to be 72.77%.

[0043] Example 4

[0044] A method for preparing yeast nucleotide chelated zinc for agricultural use is carried out according to the following steps: adding yeast nucleotide and zinc oxide into water, reacting for 80 minutes at a pH of 3.5 and a temperature of 75° C., and obtaining a filtrate to obtain liquid yeast nucleotide chelated zinc for agricultural use;

[0045] The liquid yeast nucleotide chelated zinc for agricultural use is dried to obtain the powdered yeast nucleotide chelated zinc for agricultural use.

[0046] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0047] The mass ratio of yeast nucleotides, zinc oxide and water is 20:4:76.

[0048] The zinc detection adopts EDTA titration method to determine the yield of chelated powder agricultural yeast nucleotide chelated zinc. The conversion rate of zinc into yeast nucleotide chelated zinc is calculated to be 70.87%.

[0049] Example 5

[0050] A method for preparing yeast nucleotide chelated zinc for agricultural use is carried out according to the following steps: adding yeast nucleotide and zinc sulfate monohydrate into water, reacting for 80 minutes at a pH of 3.5 and a temperature of 75° C., and obtaining a filtrate to obtain liquid yeast nucleotide chelated zinc for agricultural use;

[0051] The liquid yeast nucleotide chelated zinc for agricultural use is dried to obtain the powdered yeast nucleotide chelated zinc for agricultural use.

[0052] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0053] The mass ratio of yeast nucleotide, zinc sulfate monohydrate and water is 22:9:69.

[0054] The zinc detection adopts EDTA titration method to determine the yield of yeast nucleotide chelated zinc in chelated powder for agricultural use. The conversion rate of zinc into yeast nucleotide chelated zinc is calculated to be 69.15%.

[0055] Example 6

[0056] A method for preparing yeast nucleotide chelated ferrous iron for agricultural use is carried out according to the following steps: adding yeast nucleotide and ferrous sulfate heptahydrate into water, reacting for 80 minutes at a pH of 3.0 and a temperature of 75° C., and obtaining a filtrate to obtain liquid yeast nucleotide chelated ferrous iron for agricultural use;

[0057] The liquid yeast nucleotide chelated ferrous iron for agricultural use is dried to obtain the powdered yeast nucleotide chelated ferrous iron for agricultural use.

[0058] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0059] The mass ratio of yeast nucleotide, ferrous sulfate heptahydrate and water is 22:14:64.

[0060] The ferrous iron was detected by EDTA titration method to determine the yield of chelated powder agricultural yeast nucleotide chelated ferrous iron. The conversion rate of ferrous iron to yeast nucleotide chelated ferrous iron was calculated to be 70.51%.

[0061] Example 7

[0062] A method for preparing yeast nucleotide chelated magnesium for agricultural use, comprising the steps of adding yeast nucleotide, magnesium oxide and phosphatidylethanolamine-polyethylene glycol 2000 into water, reacting for 60 minutes at a pH of 3.0 and a temperature of 70° C., to obtain liquid yeast nucleotide chelated magnesium for agricultural use;

[0063] The liquid agricultural yeast nucleotide chelated magnesium is dried to obtain the powdered agricultural yeast nucleotide chelated magnesium.

[0064] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0065] The mass ratio of yeast nucleotides, magnesium oxide, phosphatidylethanolamine-polyethylene glycol 2000 and water is 18:1.8:1:79.2.

[0066] The magnesium was detected by EDTA titration method to determine the yield of the chelated powder agricultural yeast nucleotide chelated magnesium. The conversion rate of magnesium into yeast nucleotide chelated magnesium was calculated to be 49.93%.

[0067] Example 8

[0068] A method for preparing yeast nucleotide chelated magnesium for agricultural use is carried out according to the following steps: adding yeast nucleotide and magnesium oxide into water, reacting for 60 minutes at a pH of 3.0 and a temperature of 70° C., to obtain product A;

[0069] Add phosphatidylethanolamine-polyethylene glycol 2000 into water at a mass ratio of 10:90, and dissolve at a temperature of 50-70° C. to obtain product B;

[0070] Mix product A and product B in a mass ratio of 10:90 to obtain liquid agricultural yeast nucleotide chelated magnesium;

[0071] The liquid agricultural yeast nucleotide chelated magnesium is dried to obtain the powdered agricultural yeast nucleotide chelated magnesium.

[0072] The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, is extracted from Candida, and is purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0073] The mass ratio of yeast nucleotides, magnesium oxide and water is 20:2:78.

[0074] The magnesium detection adopts EDTA titration method to determine the yield of chelated powder agricultural yeast nucleotide chelated magnesium. The conversion rate of magnesium into yeast nucleotide chelated magnesium is calculated to be 69.58%.

[0075] The beneficial effects of the present invention are further illustrated below in conjunction with test data:

[0076] Experiment 1

[0077] 1.1 Test location: Ban Lao Shi Special Fertilizer Research and Design Institute (Henan) Co., Ltd.

[0078] 1.2 Experimental test: magnesium conversion rate (%).

[0079] 1.3 Test materials: Comparison 1 (except pH is 2.0, all others are consistent with Example 1), Comparison 2 (except pH is 2.5, all others are consistent with Example 1), Comparison 3 (except pH is 4.0, all others are consistent with Example 1), Comparison 3 (except pH is 4.5, all others are consistent with Example 1), Example 1.

[0080] 1.4 Experimental methods:

[0081] EDTA titration method was used.

[0082] 1.5 Results and Analysis

[0083] Magnesium conversion rate (%) see Table 1

[0084] Table 1

[0085] Comparison 1 Comparison 2 Example 1 Example 2 Contrast 3 Contrast 4 Magnesium conversion rate (%) 67.57 68.89 72.33 72.16 71..35 70.95

[0086] From the data in Table 1, it can be seen that the yield of yeast nucleotide chelated magnesium is closely related to pH, and yeast nucleotide chelated magnesium is not stable. When the pH is low, it is affected by H + Competition for the molecule is facilitated by dissociation at high pH, ​​resulting in inconsistent yields, with the highest yields at pH 3.0 and 3.5.

[0087] Experiment 2

[0088] 1.1 Test location: Mabei Village, Matou Town, Xiayi County, Shangqiu City, Henan Province. Peanut variety: Yuhua No. 8.

[0089] 1.2 Experimental testing: Statistical analysis of peanut yield (kg / mu, on a dry basis) and oil yield (%, on a dry basis).

[0090] 1.3 Test materials: Comparison 1 (magnesium sulfate pentahydrate), Comparison 2 (magnesium disodium ethylenediaminetetraacetate), Example 1, Example 6 and Example 7, wherein 225g of magnesium sulfate pentahydrate is divided into three portions, each portion is 75g, and Comparison 2 (magnesium disodium ethylenediaminetetraacetate)

[0091] 483g, divided into three portions, each portion is 161g, Example 1, Example 7 and Example 8 are each 300g, divided into three portions, each portion is 100g.

[0092] 1.4 Experimental methods:

[0093] Two mu of peanuts were divided into five plots, each with 200 square meters. Isolation belts were set up between the plots. The plots corresponded to the treatments randomly. The peanuts were treated with foliage three times, and one test sample was used for each treatment. Usage method: dilute one sample 1500 times with water and spray it on the leaves. Carry out the second treatment at an interval of 15 days, and then carry out the third treatment 15 days after the second treatment. The second and third treatment methods are the same as the first.

[0094] Yield statistics: After drying the peanuts for 8 days, shell them, weigh the peanut kernels, and test the moisture content of the peanut kernels. Finally, the yield is calculated on a dry basis. For example: if the weight of the peanut kernels is m, and the moisture content is 9%, the dry basis is calculated as m*

[0095] (1-0.09). The oil yield was calculated using the conventional pressing method and was equal to the mass of peanut oil divided by the mass of peanuts on a dry basis.

[0096] The normal oil yield is the mass of peanuts divided by the water content, but the oil yield in this experiment is calculated on a dry basis, so the oil yield is higher than the normal calculation method.

[0097] Except for the experimental treatment, other operations were the same in this experiment.

[0098] 2 Results and analysis

[0099] The yield (kg / mu, on a dry basis) and oil yield (%, on a dry basis) of peanuts are shown in Table 2

[0100] Table 2

[0101] Yield (kg / mu) Oil yield (%) Comparison 1 295.8 45.6 Comparison 2 331.8 47.3 Example 1 348.2 49.6 Example 7 327.4 47.9 Example 8 367.3 51.4

[0102] From the data comparison of Comparison 1, Comparison 2 and Example 1 in Table 2, it can be seen that the agricultural yeast nucleotide chelated magnesium prepared in Example 1 is better than inorganic magnesium and EDTA chelated magnesium in terms of both yield and oil yield, that is, agricultural yeast nucleotide chelated magnesium has a very broad prospect in agriculture.

[0103] Comparison of magnesium conversion rates in Table 1: From the comparison of the data of Example 1, Example 7 and Example 8, it can be seen that the addition of phosphatidylethanolamine-polyethylene glycol 2000 affects the conversion rate of magnesium into yeast nucleotide chelated magnesium. Both Example 7 and Example 8 showed a decrease in conversion rate, among which Example 8 showed a relatively smaller decrease.

[0104] Furthermore, the data of Example 1, Example 7 and Example 8 are compared, and the effect of Example 8 is more prominent. The possible reason for this is that phosphatidylethanolamine-polyethylene glycol 2000, yeast nucleotides and magnesium oxide are added at the same time, and phosphatidylethanolamine-polyethylene glycol 2000 encapsulates yeast nucleotides and magnesium ions, thereby affecting the yield; in terms of effect, since low acidity in agriculture will cause harm to plants, and it is used after dilution, the pH is close to neutral, which may cause dissociation of yeast nucleotide chelated magnesium, and the addition of phosphatidylethanolamine-polyethylene glycol 2000 may protect yeast nucleotide chelated magnesium, so the effect is better than that of Example 1 without addition.

[0105] From the comparison of the data of Comparison 2 and Example 1 in Table 2, it can be seen that the agricultural yeast nucleotide chelated magnesium prepared in the present application is better than the traditional EDTA chelated magnesium. The possible reason is that the yeast nucleotide contains nucleic acids, nucleotides, polypeptides, flavoring agents and polysaccharides, that is, it also provides life substances to plants. Therefore, compared with traditional EDTA chelated products, the use of yeast nucleotide chelation is more promising.

Claims

1. A method for preparing trace elements in agricultural yeast nucleotide chelate, characterized in that: According to the following steps, yeast nucleotide and trace element oxides or salts are added to water, and reacted for 20 to 100 minutes at a pH of 3.0 to 3.5 and a temperature of 20 to 80° C. to obtain liquid agricultural yeast nucleotide chelated trace elements; The liquid agricultural yeast nucleotide chelated trace elements are dried to obtain the powdered agricultural yeast nucleotide chelated trace elements.

2. The method for preparing trace elements in agricultural yeast nucleotide chelate according to claim 1, characterized in that: The trace elements are iron, zinc, calcium and magnesium; the salt is one or more of sulfate, nitrate and chloride.

3. The method for preparing trace elements in agricultural yeast nucleotide chelate according to claim 1, characterized in that: The yeast nucleotide has a nucleotide content of greater than or equal to 5%, a nucleic acid content of greater than or equal to 10%, and is extracted from Candida.

4. The method for preparing trace elements in agricultural yeast nucleotide chelate according to claim 1, characterized in that: The mass ratio of yeast nucleotide, trace element oxide or salt and water is 15-30:1-20:50-84.

5. The method for preparing trace elements in agricultural yeast nucleotide chelate according to any one of claims 1 to 4, characterized in that: Also included is phosphatidylethanolamine-polyethylene glycol 2000.

6. The method for preparing trace elements in agricultural yeast nucleotide chelate according to claim 5, characterized in that: According to the following steps, yeast nucleotides and trace element oxides or salts are dissolved in water, and reacted at a pH of 3.0 to 3.5 and a temperature of 20 to 80°C for 20 to 100 minutes to obtain product A: Add phosphatidylethanolamine-polyethylene glycol 2000 into water and dissolve it at a temperature of 50-80°C to obtain product B; Mix product A and product B to obtain liquid agricultural yeast nucleotide chelated trace elements; The liquid agricultural yeast nucleotide chelated trace elements are dried to obtain the powdered agricultural yeast nucleotide chelated trace elements.

7. The method for preparing trace elements in agricultural yeast nucleotide chelate according to claim 5, characterized in that: The mass ratio of yeast nucleotides, trace element oxides or salts and water is 15-30:1-20:50-84; The mass ratio of phosphatidylethanolamine-polyethylene glycol 2000 and water is 5-20:80-95; The mass ratio of product A to product B is 90-98:2-10.