Microbial fertilizer for improving transplanting survival rate of ornamental seedlings

Abstract

The invention relates to a microbial fertilizer for improving the transplanting survival rate of ornamental seedlings, and belongs to the technical field of microbial fertilizers. The microbial fertilizer is prepared from the following components in parts by weight: 2 to 3 parts of bacillus mucilaginosus, 1.5 to 2.5 parts of paecilomyces lilacinus, 1.5 to 2 parts of streptomyces microflavus, 1 to 1.5 parts of candida utilis, 15 to 20 parts of edible mushroom bran, 5 to 8 parts of water, 10 to 12 parts of potassium humate, 0.1 to 0.15 part of indolebutyric acid, 0.2 to 0.3 part of compound sodium nitrophenolate and 3 to 5 parts of polyglutamic acid. The composition is prepared from 10-12 parts by weight of aloe extract, 10-12 parts by weight of brown algae extract and 20-30 parts by weight of a water-retaining agent. By applying the microbial fertilizer, the transplanting survival rate of ornamental seedlings can be obviously increased, the biomass of the ornamental seedlings is increased, and growth of the ornamental seedlings is promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fertilizers and relates to a microbial fertilizer for improving the survival rate of transplanted ornamental seedlings. Background Art

[0002] In parks, streets, communities, and almost every corner of our lives, we cannot do without seedlings, especially ornamental seedlings. Ornamental seedlings are the most common greening tree species in our lives and are also a type of seedling widely favored by landscaping projects.

[0003] In the greening construction process, seedling transplanting is a key link. Using scientific planting technology to improve the survival rate of seedlings can not only reduce project costs, but also effectively present greening landscape effects, and achieve both economic and social benefits. Therefore, in the greening construction process, it is very important to effectively ensure the survival rate of seedlings. Seedlings are living organisms. There are many factors that affect their survival rate during the planting process. They are not only affected by environmental factors such as soil, climate, and light, but also by tree species, tree age, transplantation time, handling, and post-planting maintenance.

[0004] The factors that affect the survival rate of seedlings are mainly internal factors and external factors. The internal factors mainly refer to the problems of the seedlings themselves, such as whether the seedlings are strong before transplantation, whether they are infected with pests and diseases, whether they have entered the decline period, and whether the water and fertilizer absorbed by the roots of the seedlings after transplantation are balanced with the photosynthesis, respiration and transpiration consumption of the aboveground leaves. Practice has shown that the main reason for the death of seedlings after transplantation is that the roots cannot fully absorb water, the transpiration of stems and leaves is large, and the water balance is unbalanced. Environmental factors in external factors include climate, soil, etc. Different seedlings have different suitable growth environments. Generally, the climate, water and soil of the transplanted site are quite different from those of the original growth site. After transplantation, the seedlings may not adapt to the new environment (such as soil pH, looseness, drainage, etc.), and the survival rate of seedlings with poor adaptability is greatly reduced. Technical factors involve the selection of seedlings, the improvement of the transplant environment, the size of the soil ball, the shape and size of the planting hole, the loss of water during transportation, and the management of water and fertilizer after planting, all of which will affect the survival rate of seedlings. For example, if the soil ball is not brought when digging or the soil ball is too small, the root system will be damaged. The recovery period for minor injuries is short, and the recovery period for severe injuries is long, and some may even cause death. If the seedlings are not covered during transportation and there is serious water loss problem, the tree metabolism will decrease and the tree will become weak. During the cultivation process, if the seedlings are watered a lot or not watered at all, the roots of the seedlings will lack oxygen and die. Summary of the invention

[0005] The main purpose of the present invention is to provide a microbial fertilizer, which can significantly improve the survival rate of transplanted seedlings, especially ornamental seedlings.

[0006] The present invention adopts the following technical solutions to achieve the above purpose:

[0007] A microbial fertilizer, the preparation method of the microbial fertilizer is:

[0008] Step A, preparing aloe extract: adding water to aloe to make a homogenate, adjusting the pH to 4.5-5.0, adding cellulase and pectinase, enzymolysis at 40-50°C for 3-5h, then adding xylanase, enzymolysis at 35-40°C for 1-2h, heating to inactivate the enzyme, cooling and centrifuging, concentrating the supernatant, and drying to obtain the aloe extract; drying the aloe residue for later use;

[0009] Step B, preparing brown algae extract: adding water to brown algae to prepare homogenate, adding cellulase and Bacillus subtilis fibrinolytic enzyme, performing enzymolysis at 35-40°C for 1-3h, heating to inactivate enzymes, cooling and centrifuging, concentrating the supernatant, and drying to obtain the brown algae extract; and drying the brown algae residue for later use;

[0010] Step C, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 3-5% potassium permanganate solution by mass for 1-1.5 hours, then calcining the powder in an oxygen-free environment at 500-600° C. for 2.5-3 hours, cooling to room temperature, and washing to neutrality to obtain oyster shell biochar;

[0011] Step D, preparing a water retaining agent: adding water to aloe residue, brown algae residue, and rice husk powder, and stirring at 80-90° C. until viscous to obtain a mixture I; adding acrylamide, acrylic acid, N,N-dimethylbisacrylamide, and oyster shell biochar to a potassium hydroxide solution with a mass fraction of 10-15%, and ultrasonicating to obtain a mixed solution; adding mixture I to the mixed solution, adding ammonium persulfate, stirring at 60-70° C. until viscous, adding polyethylene glycol, drying, and crushing to obtain a water retaining agent;

[0012] Step E, mixing Bacillus gelatinus, Paecilomyces lilacinus, Streptomyces tenuifolius, Candida utilis, edible fungus bran and water, fermenting at 30-35° C. for 1.5-2 days, cooling, adding potassium humate, indolebutyric acid, sodium nitrophenolate, polyglutamic acid, aloe extract, brown algae extract and water retaining agent to obtain microbial fertilizer.

[0013] Furthermore, in the above step A, the material-liquid ratio of aloe vera to water is 1:(3-5) in kg / L;

[0014] Furthermore, in the above step A, the amount of cellulase added is 8-10 g / L, the amount of pectinase added is 12-15 g / L, and the amount of xylanase added is 8-12 g / L.

[0015] Furthermore, in the above step A, the enzyme activity of cellulase is 100000u / g, the enzyme activity of pectinase is 30000u / g, and the enzyme activity of xylanase is 10000u / g.

[0016] Furthermore, in the above step B, the liquid ratio of brown algae to water is 1:(3-5) in kg / L.

[0017] Furthermore, in the above step B, the amount of cellulase added is 10-15 g / L, and the amount of Bacillus subtilis fibrinolytic enzyme added is 8-12 g / L.

[0018] Furthermore, in the above step B, the enzymatic activity of the cellulase is 100,000 u / g, and the specification of the Bacillus subtilis fibrinolytic enzyme is 20,000 FU / 100 g.

[0019] Furthermore, the usage amount of each component in the above step D is 3-5 parts by weight of aloe residue, 8-10 parts by weight of brown algae residue, 5-7 parts by weight of rice husk powder, 100 parts by weight of water; 100 parts by weight of potassium hydroxide solution, 8-10 parts by weight of acrylamide, 7-10 parts by weight of acrylic acid, 0.5-0.8 parts by weight of N,N-dimethylbisacrylamide, 3-5 parts by weight of oyster shell biochar; 3-3.5 parts by weight of ammonium persulfate; and 15-20 parts by weight of polyethylene glycol.

[0020] Furthermore, the usage amount of each component in the above step E is 2-3 parts by weight of Bacillus subtilis, 1.5-2.5 parts by weight of Paecilomyces lilacinus, 1.5-2 parts by weight of Streptomyces tenuifolius, 1-1.5 parts by weight of Candida utilis, 15-20 parts by weight of edible fungus bran, 5-8 parts by weight of water, 10-12 parts by weight of potassium humate, 0.1-0.15 parts by weight of indolebutyric acid, 0.2-0.3 parts by weight of sodium nitrophenolate, 3-5 parts by weight of polyglutamic acid, 10-12 parts by weight of aloe extract, 10-12 parts by weight of brown algae extract, and 20-30 parts by weight of water retaining agent.

[0021] Furthermore, in the above step E, the effective live bacteria content of Bacillus mucilaginosus is 10 billion CFU / g, the effective live bacteria content of Paecilomyces lilacinus is 1 billion / g, the effective live bacteria content of Streptomyces tenuifolius is 10 billion / g, and the effective live bacteria content of Candida utilis is 10 billion CFU / g.

[0022] Furthermore, the total number of effective live bacteria in the microbial fertilizer obtained in step E is ≥ 3×10 10 Pieces / g.

[0023] The microbial fertilizer of the present invention can significantly improve the survival rate of transplanted seedlings and increase their biomass.

[0024] The present invention has the following beneficial effects:

[0025] The invention contains a variety of beneficial bacteria, which are not antagonistic but synergistic, improve the physical and chemical properties of the soil, increase soil fertility, and assist the roots of seedlings to absorb nutrients. The beneficial bacteria used can produce and secrete a variety of plant hormones such as cytokinins during the growth and reproduction process, which can promote the cell division and growth of the roots of the seedlings and promote the development of the root system. Aloe vera and brown algae extracts are added to the bacterial fertilizer of the invention. Aloe vera contains a variety of active ingredients such as polysaccharides, rhamnolide, and aloe phenol, and brown algae contains a variety of active ingredients such as polysaccharides and brown algae polyphenols. Enzymatic hydrolysis is used to fully dissolve the active ingredients. The polysaccharide components can provide nutrients for the beneficial bacteria in the invention to ensure their growth and reproduction. On the other hand, the presence of the active ingredients can also promote the growth of the roots of the seedlings. The residue after enzymatic hydrolysis and oyster shell biochar are used to prepare a water retaining agent, which can improve soil properties and prevent nutrient loss.

[0026] The use of the microbial fertilizer of the present invention can regulate soil nutrients, so that the transplanted seedlings can quickly adapt to the environment, restore root vitality and promote root growth, improve the quality of the seedlings, increase their survival rate, and at the same time significantly increase the biomass of the seedlings and promote their growth. DETAILED DESCRIPTION

[0027] The present invention is further illustrated below in conjunction with specific examples, and it should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications to various equivalent forms of the present invention by those skilled in the art all fall within the scope protected by the claims of the present application. The hydrolases and microorganisms used in the following embodiments are all existing commercially available products, and can be purchased through online or offline channels.

[0028] Example 1

[0029] Step A, preparing aloe extract: adding 5L of water to 1kg of aloe to prepare a homogenate, adjusting the pH to 4.5, adding 10g / L of cellulase with an enzyme activity of 100000u / g and 12g / L of pectinase with an enzyme activity of 30000u / g, enzymolysis at 50°C for 3h, then adding 12g / L of xylanase with an enzyme activity of 10000u / g, enzymolysis at 40°C for 1h, heating to inactivate the enzyme, cooling and centrifuging, concentrating the supernatant, and drying to obtain the aloe extract; the aloe residue is dried for later use;

[0030] Step B, preparing brown algae extract: 1 kg of brown algae is added with 5 L of water to prepare a homogenate, 15 g / L of cellulase with an enzyme activity of 100,000 u / g and 8 g / L of Bacillus subtilis fibrinolytic enzyme with a specification of 20,000 FU / 100 g are added, enzymolysis is carried out at 40° C. for 1 hour, the enzyme is heated to inactivate the enzyme, the supernatant is concentrated and dried to obtain the brown algae extract; the brown algae residue is dried and set aside;

[0031] Step C, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 5% potassium permanganate solution for 1 hour, and then calcining the powder in an oxygen-free environment at 600° C. for 2.5 hours, cooling to room temperature, and washing to neutrality to obtain oyster shell biochar;

[0032] Step D, preparing a water retaining agent: adding 5 kg of aloe residue, 8 kg of brown algae residue, and 7 kg of rice husk powder to 100 kg of water, and stirring at 90° C. until viscous to obtain a mixture I; adding 8 kg of acrylamide, 10 kg of acrylic acid, 0.5 kg of N,N-dimethylbisacrylamide, and 5 kg of oyster shell biochar to 100 kg of a potassium hydroxide solution with a mass fraction of 15%, and ultrasonically treating to obtain a mixed solution; adding mixture I to the mixed solution, adding 3 kg of ammonium persulfate, stirring at 70° C. until viscous, adding 15 kg of polyethylene glycol, drying, and crushing to obtain a water retaining agent;

[0033] Step E, mixing 3kg of Bacillus subtilis with an effective live bacteria content of 10 billion CFU / g, 1.5kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 2kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 20kg of edible fungus bran, and 5kg of water, fermenting at 35°C for 1.5 days, cooling, adding 12kg of potassium humate, 0.1kg of indolebutyric acid, 0.2kg of sodium nitrophenolate, 5kg of polyglutamic acid, 10kg of aloe extract, 12kg of brown algae extract, and 20kg of water retaining agent, mixing, drying, and crushing to obtain microbial fertilizer.

[0034] Example 2

[0035] Step A, preparing aloe extract: adding 3L of water to 1kg of aloe to prepare a homogenate, adjusting the pH to 5.0, adding 8g / L of cellulase with an enzyme activity of 100000u / g and 15g / L of pectinase with an enzyme activity of 30000u / g, enzymolysis at 40°C for 5h, then adding 8g / L of xylanase with an enzyme activity of 10000u / g, enzymolysis at 35°C for 2h, heating to inactivate the enzyme, cooling and centrifuging, concentrating the supernatant, and drying to obtain the aloe extract; the aloe residue is dried for later use;

[0036] Step B, preparing brown algae extract: 1 kg of brown algae is added with 3 L of water to prepare a homogenate, 10 g / L of cellulase with an enzyme activity of 100,000 u / g and 12 g / L of Bacillus subtilis fibrinolytic enzyme with a specification of 20,000 FU / 100 g are added, enzymolysis is carried out at 35° C. for 3 h, the enzyme is heated to inactivate the enzyme, the supernatant is concentrated and dried to obtain the brown algae extract; the brown algae residue is dried and set aside;

[0037] Step C, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 3% potassium permanganate solution for 1.5 hours, then calcining the powder in an oxygen-free environment at 500° C. for 3 hours, cooling to room temperature, and washing the powder until neutral to obtain oyster shell biochar;

[0038] Step D, preparing a water retaining agent: adding 3 kg of aloe residue, 10 kg of brown algae residue, and 5 kg of rice husk powder to 100 kg of water and mixing, stirring at 80° C. until viscous, to obtain a mixture I; adding 10 kg of acrylamide, 7 kg of acrylic acid, 0.8 kg of N,N-dimethylbisacrylamide, and 3 kg of oyster shell biochar to 100 kg of a 10% potassium hydroxide solution by mass fraction, and ultrasonically treating to obtain a mixed solution; adding mixture I to the mixed solution, adding 3.5 kg of ammonium persulfate, stirring at 60° C. until viscous, adding 20 kg of polyethylene glycol, drying, and crushing to obtain a water retaining agent;

[0039] Step E, mixing 2kg of Bacillus subtilis with an effective live bacteria content of 10 billion CFU / g, 2.5kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 1.5kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1.5kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 15kg of edible fungus bran, and 8kg of water, fermenting at 30°C for 2 days, cooling, adding 10kg of potassium humate, 0.15kg of indolebutyric acid, 0.3kg of sodium nitrophenolate, 3kg of polyglutamic acid, 12kg of aloe extract, 10kg of brown algae extract, and 30kg of water retaining agent, mixing, drying, and crushing to obtain microbial fertilizer.

[0040] Example 3

[0041] Step A, preparing aloe extract: 1 kg of aloe vera is added with 4 L of water to prepare a homogenate, the pH is adjusted to 4.5, 9 g / L of cellulase with an enzyme activity of 100,000 u / g and 13 g / L of pectinase with an enzyme activity of 30,000 u / g are added, and enzymolysis is carried out at 50° C. for 4 hours, and then 10 g / L of xylanase with an enzyme activity of 10,000 u / g is added, and enzymolysis is carried out at 40° C. for 1.5 hours, the enzyme is inactivated by heating, and the supernatant is concentrated and dried to obtain the aloe vera extract; the aloe vera residue is dried and reserved;

[0042] Step B, preparing brown algae extract: 1 kg of brown algae is added with 4 L of water to prepare a homogenate, 13 g / L of cellulase with an enzyme activity of 100,000 u / g and 10 g / L of Bacillus subtilis fibrinolytic enzyme with a specification of 20,000 FU / 100 g are added, enzymolysis is carried out at 40° C. for 2 h, the enzyme is heated to inactivate the enzyme, the supernatant is concentrated and dried to obtain the brown algae extract; the brown algae residue is dried and set aside;

[0043] Step C, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 4% potassium permanganate solution for 1.5 hours, then calcining the powder in an oxygen-free environment at 600° C. for 2.5 hours, cooling to room temperature, and washing to neutrality to obtain oyster shell biochar;

[0044] Step D, preparing a water retaining agent: adding 100 kg of water to 4 kg of aloe residue, 9 kg of brown algae residue, and 6 kg of rice husk powder, and mixing them at 90° C. until they become viscous, to obtain a mixture I; adding 9 kg of acrylamide, 8 kg of acrylic acid, 0.7 kg of N,N-dimethylbisacrylamide, and 4 kg of oyster shell biochar to 100 kg of a potassium hydroxide solution having a mass fraction of 13%, and ultrasonically treating the mixture to obtain a mixed solution; adding the mixture I to the mixed solution, adding 3.3 kg of ammonium persulfate, stirring at 70° C. until it becomes viscous, adding 18 kg of polyethylene glycol, drying, and crushing to obtain a water retaining agent;

[0045] Step E, mixing 2.5kg of Bacillus subtilis with an effective live bacteria content of 10 billion CFU / g, 2kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 1.8kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1.3kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 18kg of edible fungus bran, and 7kg of water, fermenting at 35°C for 1.5 days, cooling, adding 11kg of potassium humate, 0.13kg of indolebutyric acid, 0.25kg of sodium nitrophenolate, 4kg of polyglutamic acid, 11kg of aloe extract, 11kg of brown algae extract, and 25kg of water retaining agent, mixing, drying, and crushing to obtain microbial fertilizer.

[0046] Comparative Example 1

[0047] Step A, preparing aloe extract: adding 4 L of water to 1 kg of aloe to prepare a homogenate, adjusting the pH to 4.5, adding 9 g / L of cellulase with an enzyme activity of 100,000 u / g and 13 g / L of pectinase with an enzyme activity of 30,000 u / g, enzymolysis at 50°C for 4 hours, then adding 10 g / L of xylanase with an enzyme activity of 10,000 u / g, enzymolysis at 40°C for 1.5 hours, heating to inactivate the enzyme, cooling and centrifuging, concentrating the supernatant, and drying to obtain the aloe extract;

[0048] Step B, preparing brown algae extract: 1 kg of brown algae is added with 4 L of water to prepare a homogenate, 13 g / L of cellulase with an enzyme activity of 100,000 u / g and 10 g / L of Bacillus subtilis fibrinolytic enzyme with a specification of 20,000 FU / 100 g is added, enzymolysis is carried out at 40° C. for 2 h, the enzyme is inactivated by heating, the mixture is cooled and centrifuged, the supernatant is concentrated and dried to obtain the brown algae extract;

[0049] Step C, mixing 2.5kg of Bacillus colloids with an effective live bacteria content of 10 billion CFU / g, 2kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 1.8kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1.3kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 18kg of edible fungus bran, and 7kg of water, fermenting the mixture at 35°C for 1.5 days, cooling the mixture, adding 11kg of potassium humate, 0.13kg of indolebutyric acid, 0.25kg of sodium nitrophenolate, 4kg of polyglutamic acid, 11kg of aloe extract, 11kg of brown algae extract, and 25kg of ammonium polyacrylate, mixing the mixture, drying the mixture, and crushing the mixture to obtain a microbial fertilizer.

[0050] Comparative Example 2

[0051] Step A, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 4% potassium permanganate solution for 1.5 hours, then calcining the powder in an oxygen-free environment at 600° C. for 2.5 hours, cooling to room temperature, and washing to neutrality to obtain oyster shell biochar;

[0052] Step B, preparing a water retaining agent: adding 9 kg of acrylamide, 8 kg of acrylic acid, 0.7 kg of N,N-dimethylbisacrylamide, and 4 kg of oyster shell biochar to 100 kg of a potassium hydroxide solution with a mass fraction of 13%, and ultrasonically treating to obtain a mixed solution; adding mixture I to the mixed solution, adding 3.3 kg of ammonium persulfate, stirring at 70° C. until viscous, drying, and crushing to obtain a water retaining agent;

[0053] Step C, mixing 2.5kg of Bacillus subtilis with an effective live bacteria content of 10 billion CFU / g, 2kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 1.8kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1.3kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 18kg of edible fungus bran, and 7kg of water, fermenting the mixture at 35°C for 1.5 days, cooling the mixture, adding 11kg of potassium humate, 0.13kg of indolebutyric acid, 0.25kg of sodium nitrophenolate, 4kg of polyglutamic acid, 11kg of aloe extract, 11kg of brown algae extract, and 25kg of a water retaining agent, mixing the mixture, drying the mixture, and crushing the mixture to obtain a microbial fertilizer.

[0054] Comparative Example 3

[0055] Step A, preparing aloe extract: 1 kg of aloe vera is added with 4 L of water to prepare a homogenate, the pH is adjusted to 4.5, 9 g / L of cellulase with an enzyme activity of 100,000 u / g and 13 g / L of pectinase with an enzyme activity of 30,000 u / g are added, enzymolysis is performed at 50° C. for 4 h, the enzyme is heated to inactivate the enzyme, the supernatant is concentrated and dried to obtain the aloe extract; the aloe residue is dried and set aside;

[0056] Step B, preparing brown algae extract: 1 kg of brown algae is added with 4 L of water to make a homogenate, 13 g / L of cellulase with an enzyme activity of 100,000 u / g is added, enzymolysis is carried out at 40° C. for 2 h, the enzyme is heated to inactivate the enzyme, the supernatant is concentrated and dried to obtain the brown algae extract; the brown algae residue is dried and set aside;

[0057] Step C, preparing a water retaining agent: adding 4 kg of aloe residue and 9 kg of brown algae residue to 100 kg of water, and stirring at 90° C. until the mixture becomes viscous to obtain a mixture I; adding 9 kg of acrylamide, 8 kg of acrylic acid, and 0.7 kg of N,N-dimethylbisacrylamide to 100 kg of a potassium hydroxide solution having a mass fraction of 13%, and ultrasonically treating the mixture to obtain a mixed solution; adding the mixture I to the mixed solution, adding 3.3 kg of ammonium persulfate, stirring at 70° C. until the mixture becomes viscous, adding 18 kg of polyethylene glycol, drying, and crushing to obtain a water retaining agent;

[0058] Step D, 2.5 kg of Bacillus subtilis with an effective live bacteria content of 10 billion CFU / g, 2 kg of Paecilomyces lilacinus with an effective live bacteria content of 1 billion / g, 1.8 kg of Streptomyces tenuiflavous with an effective live bacteria content of 10 billion / g, 1.3 kg of Candida utilis with an effective live bacteria content of 10 billion CFU / g, 18 kg of edible fungus bran, 7 kg of water, 11 kg of potassium humate, 0.13 kg of indolebutyric acid, 11 kg of aloe extract, 11 kg of brown algae extract, and 25 kg of water retaining agent, mixed, dried, and crushed to obtain microbial fertilizer.

[0059] Performance test of the effect of microbial fertilizer on transplanting of ornamental seedlings

[0060] This experimental research was carried out in an ornamental nursery base of a cooperative unit. The soil properties of the transplanting site were: pH 5.01, organic matter 13.85g / kg, total nitrogen 1.55g / kg, total potassium 13.82g / kg, alkaline-hydrolyzed nitrogen 86.1mg / kg, available phosphorus 13.6mg / kg, and available potassium 71.3mg / kg.

[0061] 1. The effect of bacterial fertilizer on the transplanting of camphor tree seedlings

[0062] One-year-old camphor tree seedlings with similar growth and plant height to the nursery base were selected for transplanting. The transplanting area was 20m 2 (5m×10m), the row spacing and plant spacing are 1m×1m; 3 days before transplanting, the soil in the transplanting area was treated in the following ways.

[0063] XZ blank area: no processing;

[0064] XZ test area 1: base application of the microbial fertilizer prepared in Example 1 (1 kg / 10 m 2 );

[0065] XZ test area 2: base application of the microbial fertilizer prepared in Example 2 (1 kg / 10 m 2 );

[0066] XZ test zone 3: base application of the microbial fertilizer prepared in Example 3 (1 kg / 10 m 2 );

[0067] XZ test area 4: base application of the microbial fertilizer prepared in comparative example 1 (1kg / 10m 2 );

[0068] XZ test area 5: base application of the microbial fertilizer prepared in comparative example 2 (1kg / 10m 2 );

[0069] XZ test area 6: base application of the microbial fertilizer prepared in comparative example 3 (1kg / 10m 2 );

[0070] Two months after the camphor tree seedlings were transplanted, urea was applied at 0.1 kg / 10 m 2 , the management of other seedlings is the same.

[0071] The camphor tree seedlings will be transplanted on April 3, 2023. After transplanting, the plant height and ground diameter of all camphor tree seedlings in each experimental plot will be measured regularly. On September 21, 2023, the survival rate of the transplanted camphor tree seedlings will be counted. At the same time, 5 camphor tree seedlings will be randomly selected from each experimental plot. After cleaning, the roots, stems and leaves will be separated, and the biomass of each part will be measured after drying in an oven.

[0072] The results and analysis are as follows:

[0073] As the number of transplanting days increased, the plant height, i.e., ground diameter, of the camphor tree seedlings in each test plot showed an increasing trend. However, there were obvious differences in the plant heights of the camphor tree seedlings that were applied with different microbial fertilizers. The plant height and ground diameter of the camphor tree seedlings that were applied with the microbial fertilizers of Examples 1-3 increased significantly.

[0074] Table 1 Changes in plant height of camphor tree seedlings

[0075]

[0076] Table 2 Changes in the ground diameter of camphor tree seedlings

[0077]

[0078]

[0079] The microbial fertilizer of the invention can obviously promote the growth of camphor tree seedlings, the biomass of the roots, stems and leaves of the camphor tree seedlings are significantly increased, and the root-crown ratio is increased.

[0080] The survival rate of the camphor tree seedlings applied with the microbial fertilizers of Examples 1-3 of the present invention after transplantation was significantly higher than that of the seedlings in other test plots.

[0081] Table 3 Biomass and survival rate of Cinnamomum camphora seedlings

[0082]

[0083] 2. Effect of bacterial fertilizer on transplanting of Phoebe chekiangensis

[0084] Select Zhejiang Nan seedlings with basically the same growth, with an average seedling height of 70.1±1.6cm and an average ground diameter of 4.11±0.53cm. Randomly divide them into 7 groups, with 50 plants in each group. Transplant them to the plots in each experimental area on March 19, 2023. Remove the bad leaves and leaves at the bottom of the seedlings before transplanting, cut the remaining leaves in half, and remove the bad broken roots and bad roots. On the 5th day after transplanting, treat them as follows:

[0085] ZJN blank area: no treatment;

[0086] ZJN test area 1: applying the microbial fertilizer prepared in Example 1 (50 g / tree);

[0087] ZJN test area 2: applying the microbial fertilizer prepared in Example 2 (50 g / tree);

[0088] ZJN test area 3: applying the microbial fertilizer prepared in Example 3 (50 g / tree);

[0089] ZJN test zone 4: applying the microbial fertilizer prepared in comparative example 1 (50 g / tree);

[0090] ZJN test zone 5: applying the microbial fertilizer prepared in comparative example 2 (50 g / tree);

[0091] ZJN test zone 6: applying the microbial fertilizer prepared in comparative example 3 (50 g / tree);

[0092] Two months after the transplanting of the Zhejiang Nan seedlings, 10g of urea per tree was applied in each transplanting plot, and the management of other seedlings was the same.

[0093] During the experiment, the number of surviving Phoebe chekiangensis and the number of new leaves in each experimental area were counted based on factual observation and counting.

[0094] On October 20, 2023, healthy and pest-free leaves of the current year were randomly selected from each experimental plot, and the chlorophyll content in the leaves was determined by spectrophotometry.

[0095] The results and analysis are as follows:

[0096] Compared with the Zhejiang Nan seedlings in the blank area, the survival rate and the number of new leaves of the Zhejiang Nan seedlings applied with microbial fertilizers were significantly increased, and the chlorophyll content in the leaves was significantly increased. Among them, the survival rate, the number of new leaves, and the chlorophyll content in the leaves of the Zhejiang Nan seedlings using the microbial fertilizers of Examples 1-3 increased significantly, indicating that the microbial fertilizer can promote the survival and growth of seedlings after transplanting and increase the photosynthesis of seedlings.

[0097] Table 4 Survival rate, number of new leaves and chlorophyll content of leaves of Phoebe chekiangensis seedlings

[0098] seedling Survival rate (%) Number of new leaves Chlorophyll content (mg / g) ZJN blank area 82 6.8 3.5 ZJN Test Zone 1 96 9.5 6.8 ZJN Test Zone 2 92 8.9 6.3 ZJN Test Zone 3 98 9.1 7.1 ZJN Test Zone 4 86 7.6 4.5 ZJN Test Zone 5 90 8.4 5.6 ZJN Test Zone 6 88 8.8 5.2

Claims

1. A microbial fertilizer for improving the survival rate of ornamental seedling transplanting, characterized in that: The preparation method of the microbial fertilizer is: Step A, preparing aloe extract: adding water to aloe to make a homogenate, adjusting the pH to 4.5-5.0, adding cellulase and pectinase, enzymolysis at 40-50°C for 3-5h, then adding xylanase, enzymolysis at 35-40°C for 1-2h, heating to inactivate the enzyme, cooling and centrifuging, concentrating the supernatant, and drying to obtain the aloe extract; drying the aloe residue for later use; Step B, preparing brown algae extract: adding water to brown algae to prepare homogenate, adding cellulase and Bacillus subtilis fibrinolytic enzyme, performing enzymolysis at 35-40°C for 1-3h, heating to inactivate enzymes, cooling and centrifuging, concentrating the supernatant, and drying to obtain the brown algae extract; and drying the brown algae residue for later use; Step C, preparing oyster shell biochar: crushing oyster shells, sieving, and obtaining oyster shell powder; soaking the oyster shell powder in a 3-5% potassium permanganate solution by mass for 1-1.5 hours, then calcining the powder in an oxygen-free environment at 500-600° C. for 2.5-3 hours, cooling to room temperature, and washing to neutrality to obtain oyster shell biochar; Step D, preparing a water retaining agent: adding water to aloe residue, brown algae residue, and rice husk powder, and stirring at 80-90° C. until viscous to obtain a mixture I; adding acrylamide, acrylic acid, N,N-dimethylbisacrylamide, and oyster shell biochar to a potassium hydroxide solution with a mass fraction of 10-15%, and ultrasonicating to obtain a mixed solution; adding mixture I to the mixed solution, adding ammonium persulfate, stirring at 60-70° C. until viscous, adding polyethylene glycol, drying, and crushing to obtain a water retaining agent; Step E, mixing Bacillus gelatinus, Paecilomyces lilacinus, Streptomyces tenuifolius, Candida utilis, edible fungus bran and water, fermenting at 30-35° C. for 1.5-2 days, cooling, adding potassium humate, indolebutyric acid, sodium nitrophenolate, polyglutamic acid, aloe extract, brown algae extract and water retaining agent to obtain microbial fertilizer.

2. The microbial fertilizer according to claim 1, characterized in that: In the step A, the solid-liquid ratio of aloe vera to water is 1:(3-5) in kg / L.

3. The microbial fertilizer according to claim 1, characterized in that In the step A, the amount of cellulase added is 8-10 g / L, the amount of pectinase added is 12-15 g / L, and the amount of xylanase added is 8-12 g / L.

4. The microbial fertilizer according to claim 1, characterized in that In the step A, the enzyme activity of the cellulase is 100,000 u / g, the enzyme activity of the pectinase is 30,000 u / g, and the enzyme activity of the xylanase is 10,000 u / g.

5. The microbial fertilizer according to claim 1, characterized in that The liquid ratio of brown algae to water in step B is 1:(3-5) in kg / L.

6. The microbial fertilizer according to claim 1, characterized in that: In the step B, the amount of cellulase added is 10-15 g / L, and the amount of Bacillus subtilis fibrinolytic enzyme added is 8-12 g / L.

7. The microbial fertilizer according to claim 1, characterized in that: The enzymatic activity of the cellulase in step B is 100,000 u / g, and the specification of the Bacillus subtilis fibrinolytic enzyme is 20,000 FU / 100 g.

8. The microbial fertilizer according to claim 1, characterized in that: The usage amount of each component in step D is 3-5 parts by weight of aloe residue, 8-10 parts by weight of brown algae residue, 5-7 parts by weight of rice husk powder, 100 parts by weight of water; 100 parts by weight of potassium hydroxide solution, 8-10 parts by weight of acrylamide, 7-10 parts by weight of acrylic acid, 0.5-0.8 parts by weight of N,N-dimethylbisacrylamide, 3-5 parts by weight of oyster shell biochar; 3-3.5 parts by weight of ammonium persulfate; Polyethylene glycol 15-20 parts by weight.

9. The microbial fertilizer according to claim 1, characterized in that: The usage amount of each component in the step E is 2-3 parts by weight of Bacillus mucilaginosus, 1.5-2.5 parts by weight of Paecilomyces lilacinus, 1.5-2 parts by weight of Streptomyces tenuiflavus, 1-1.5 parts by weight of Candida utilis, 15-20 parts by weight of edible fungus bran, 5-8 parts by weight of water, 10-12 parts by weight of potassium humate, 0.1-0.15 parts by weight of indolebutyric acid, 0.2-0.3 parts by weight of sodium nitrophenolate, 3-5 parts by weight of polyglutamic acid, 10-12 parts by weight of aloe extract, 10-12 parts by weight of brown algae extract, and 20-30 parts by weight of water retaining agent.

10. The microbial fertilizer according to claim 1, characterized in that: In the step E, the effective live bacteria content of Bacillus mucilaginosus is 10 billion CFU / g, the effective live bacteria content of Paecilomyces lilacinus is 10 billion / g, the effective live bacteria content of Streptomyces tenuiflavous is 10 billion / g, and the effective live bacteria content of Candida utilis is 10 billion CFU / g.

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