A method of preparing VB using dried orange peel fruit 12 Methods
By fermenting in the tangerine peel pulp-yeast powder medium, VB12 is produced by using Propionibacterium fermentation, the problem of post-harvest treatment of tangerine peel pulp is solved, and the efficient production of VB12 and environmentally friendly feed additives are achieved.
Patent Information
- Application Number
- CN202411908913.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-24
- Publication Date
- 2025-04-04
AI Technical Summary
The post-harvest and treatment of tangerine peel pulp makes it difficult to utilize the pulp and there is a risk of environmental pollution.
Vitamin B12 (VB12) was produced by fermenting Propionibacterium fermentation in tangerine peel-yeast powder medium. The culture medium consists of dried tangerine peel pulp juice, water and yeast powder, and achieves efficient production of VB12 through specific culture conditions (30°C, 200 rpm, 5 days).
The post-harvest problem of tangerine peel and flesh has been solved. VB12 in the fermentation broth can be used as a feed additive, which has economic and environmental benefits.
Smart Images
Figure CN119753060A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of feed processing, and particularly relates to a method for preparing VB using tangerine peel fruit. 12 method. Background Art
[0002] Guangdong dried tangerine peel is one of the top ten southern medicines in Guangdong. Its source plant is the citrus plant tea orange. Dried tangerine peel is rich in a variety of active ingredients, such as flavonoids, volatile oils, alkaloids, polysaccharides, trace elements, etc. These active substances have many pharmacologically active effects. The value of tea orange lies mainly in the peel, not the fruit. The pulp is sour and astringent and not delicious. Therefore, the post-harvest problem of dried tangerine peel and pulp has always been a focus of debate. If it is treated as a fruit, it tastes bad, and if it is treated as garbage, it is easy to increase environmental pollution. Therefore, the post-harvest treatment of tea orange pulp is an issue that fruit farmers urgently need to solve.
[0003] Therefore, using microbial agents to produce VB with tangerine peel pulp as a carbon source 12 It not only solves the post-harvest problem of tangerine peel pulp, but also can use it as feed production, and can also reduce its pollution to the environment. Summary of the invention
[0004] The present invention aims at the post-harvest problem of tangerine peel pulp and discloses a method for producing vitamin B12 by using tangerine peel pulp as a carbon source. 12 (VB 12 ) method.
[0005] To achieve the above-mentioned purpose, the present invention adopts the following technical solutions:
[0006] A method of preparing VB using dried orange peel fruit 12 The method comprises the following steps: inoculating Propionibacterium freudenreichii into a tangerine peel pulp-yeast powder culture medium for cultivation, wherein the formula of the tangerine peel pulp-yeast powder culture medium is: the volume ratio of tangerine peel pulp juice to water is 3:1-1:3, the yeast powder is 5-10g / L, and the solvent is water.
[0007] Preferably, the formula of the tangerine peel pulp-yeast powder culture medium is: the volume ratio of tangerine peel pulp juice to water is 1:1, the yeast powder is 5g / L, and the solvent is water.
[0008] Preferably, the tangerine peel pulp juice is obtained by removing the peel of tangerine peel fruits and then directly squeezing the pulp.
[0009] Preferably, the Propionibacterium freudenreichii is Propionibacterium freudenreichii CICC 10019, which is deposited in the China Industrial Microbiological Culture Collection Center (CICC), with the strain number: CICC 10019, and the address: Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing (100015).
[0010] Preferably, the culture conditions are culturing at 30° C. and 200 rpm for 5 days.
[0011] On the other hand, the present invention also provides the above method for preparing VB 12 Purpose.
[0012] The present invention has the following beneficial effects: the present invention uses tangerine peel pulp as a carbon source for microbial culture, supplements a certain amount of yeast powder as a nitrogen source, and prepares a microbial culture medium suitable for preparing VB by fermentation of Propionibacterium freudenreichii. 12 The yield of tangerine peel pulp-yeast powder medium reached 19.8μg / L on the 5th day, which solved the post-harvest problem of tangerine peel pulp. The fermentation liquid can be used as a feed additive to supplement VB 12 , which has certain economic and environmental benefits. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 To culture Propionibacterium freudenreichii CICC10019 in tangerine peel pulp-yeast powder medium and RCM medium (control) to produce VB 12 Note: Different capital letters indicate significant differences among different treatments at the p≤0.05 level. DETAILED DESCRIPTION
[0014] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.
[0015] Example 1
[0016] Detecting VB 12 The method was high performance liquid chromatography, and the detection conditions were: C18-250A chromatographic column (4.6mm×150mm, 5μm), the mobile phase was methanol:water=28:72 (volume ratio), isocratic elution, the elution flow rate was 0.8mL / min, the absorption wavelength was 361nm, the column temperature was 38℃, and the injection volume was 10μL.
[0017] The experimental control was Reinforced Clostridium Medium (RCM). The formula of RCM medium was as follows: 10.0 g beef extract powder, 5.0 g peptone, 3.0 g yeast extract powder, 5.0 g glucose, 1.0 g soluble starch, 5.0 g NaCl, 3.0 g NaAc, 0.5 g L-cysteine hydrochloride, and 1000 mL distilled water.
[0018] The steps for preparing the tangerine peel pulp-yeast powder culture medium are as follows: process the tangerine peel pulp into juice using a juicer, take 100 mL of tangerine peel juice and add an equal volume of distilled water, add 1 g of yeast powder, stir evenly, and sterilize at high temperature and high pressure to obtain the culture medium.
[0019] Propionibacterium freudenreichii (strain number: CICC10019) purchased from the China Industrial Microbiological Culture Collection (CICC) was streaked on RCM solid medium, cultured at 30°C in an anaerobic bag for 24 hours, activated the strain, picked a single colony and transferred it to RCM liquid medium, cultured at 30°C and 200rpm for 16 hours. Then it was inoculated into the above medium at a volume ratio of 5%, cultured at 30°C and 200rpm for 5 days, and the RCM medium was used as a control to measure its production of VB under the culture of tangerine peel pulp-yeast powder medium. 12 situation.
[0020] Figure 1 It showed that VB could be produced by fermentation with tangerine peel pulp as carbon source and yeast powder as nitrogen source by Propionibacterium freudenreichii 12 The yield reached 12.6 μg / L on the third day and 19.8 μg / L on the fifth day. It can be seen that tangerine peel pulp can be used as a carbon source to ferment and produce VB 12 , which further expands the use of the flesh after harvesting tangerine peel.
[0021] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. Preparation of VB using dried orange peel fruit 12 The method is characterized in that The following steps are involved: The method is prepared by inoculating Propionibacterium freudenreichii into a tangerine peel pulp-yeast powder culture medium for cultivation. The formula of the tangerine peel pulp-yeast powder culture medium is as follows: the volume ratio of tangerine peel pulp juice to water is 3:1-1:3, the yeast powder is 5-10 g / L, and the solvent is water.
2. The method for preparing VB using dried orange peel fruit according to claim 1 12 The method is characterized in that The formula of the tangerine peel pulp-yeast powder culture medium is as follows: the volume ratio of tangerine peel pulp juice to water is 1:1, the yeast powder is 5g / L, and the solvent is water.
3. The method for preparing VB using dried orange peel fruit according to claim 1 or 2. 12 The method is characterized in that The tangerine peel pulp juice is obtained by removing the peel of tangerine peel fruits and then directly squeezing the pulp.
4. The method for preparing VB using dried orange peel fruit according to claim 1 12 The method is characterized in that The Propionibacterium freudenreichii is Propionibacterium freudenreichii CICC 10019.
5. The method for preparing VB using dried orange peel fruit according to claim 1 12 The method is characterized in that The culture conditions are culturing at 30° C. and 200 rpm for 5 days.
6. The method according to any one of claims 1 to 5 for preparing VB 12 Purpose.