A method for preparing marine oligopeptide with uric acid-lowering activity

Abstract

The present invention provides a method for preparing a marine oligopeptide with uric acid-lowering activity, comprising the following preparation steps: S1, raw material processing; S2, the fish head, viscera, and fish tail of tuna are fully rotated and cleaned, and disinfected, and then the fish head, viscera, and tail are stirred and chopped into substance B at high speed; S3, the processed fish meat is stirred and homogenized at high speed to be processed into substance A; S4, 1-10 parts of alkaline protease and 2-8 parts of neutral protease are added, and then the two are proportioned together; S5, the mixture of the above step is subjected to low-temperature centrifugation for 10-30min protease mixed solution; S6, the above liquid is filtered and concentrated; S7, the above liquid is spray-dried and sprayed into fine droplets through a nozzle. By cleaning the impurities outside the fresh tuna in advance, and recycling the cleaned wastewater, it is prevented that there are many impurities affecting the preparation of the oligopeptide, HPRT enzyme and LDH enzyme are added, and the protease is matched to enable it to reduce the uric acid activity.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of marine oligopeptides, in particular to a method for preparing a marine oligopeptide with uric acid-lowering activity. Background Art

[0002] Tuna is an animal of the genus Tuna in the family Tuna of the order Perciformes. It is also known as bomb fish, plumb bob, flower cigarette, three-spot fish, white tuna, etc. The body of tuna is spindle-shaped, and the cross section is nearly circular. The head is conical, slightly flattened, and the snout is pointed. The back of the body is blue-black, and the abdomen is silver-gray. The body surface is only covered with round scales on the chest armor and the front of the lateral line. The scales above the pectoral fins are slightly larger. Tuna is distributed in India and the Western Pacific Ocean. In China, it is distributed in the Yellow Sea, the East China Sea, and the South China Sea. Tuna usually lives in the coral reefs in the offshore area. It likes to gather in groups and swim quickly. Tuna is a carnivorous fish. The bait is mainly various small fish, shrimps and zooplankton in the upper layer. It often preys on fish such as blue round tuna, green scale, fat eye fish, round belly herring, as well as hair shrimp, squid, cuttlefish, etc. April to August is the spawning period of tuna, and June and July are generally the peak production period. Tuna spawns mostly in reefs, sandy areas, and areas with algae. The life span of tuna is about 20 years. Tuna is a large economic fish with rich meat. It can be eaten fresh or pickled into salted fish. It is also a high-quality raw material for the canning industry. Fresh tuna is easy to smell and deteriorate, so it is necessary to pay attention to processing and preservation technology. Fresh tuna can be made into sashimi. Tuna has high protein content, low water content, and is rich in a variety of fat-soluble and water-soluble vitamins, which can promote growth and delay aging. In particular, it contains high amounts of EPA and DHA, which can effectively protect the heart and blood vessels. Marine fish oligopeptides can effectively solve the problem of reduced moisture in the skin and muscles. Timely supplementation of collagen peptides can keep the skin young; it can tightly bind calcium to bone cells and prevent them from being lost or degenerated; it can keep the cornea moist and transparent, and increase eye transparency; it can promote tight connection between muscle cells, making them elastic and shiny; it can regulate endocrine, protect and strengthen visceral functions; it can bind to immunoglobulins and increase immunity. Tuna is a low-fat, high-protein seafood with rich nutritional value, so a method for preparing marine oligopeptides with uric acid-lowering activity is provided.

[0003] When preparing marine oligopeptides with uric acid-lowering activity, the prior art uses marine fish for preparation, but wastes water in the process of cleaning impurities from the marine fish, and there are many impurities in the process of mincing the fish meat, fish head, viscera, and fish tail, which affect the preparation of the oligopeptides. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides a method for preparing a marine oligopeptide with uric acid-lowering activity, which solves the problem of water waste in the process of cleaning marine fish and the problem that mincing fish meat, fish heads, viscera and fish tails together easily affects the preparation of oligopeptides.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented by the following technical scheme: a method for preparing a marine oligopeptide with uric acid-lowering activity, comprising the following preparation steps:

[0008] S1. Raw material processing: Select fresh tuna, first use clean water to roughly clean the mud and sand stains on its surface, and use a scraper to scrape off the tightly bound impurities, and then use a special knife to scrape off the scales;

[0009] S2, taking out the head, viscera and tail of the tuna separately, cleaning the fish meat and placing it, rotating the head, viscera and tail of the tuna thoroughly to clean them, disinfecting them, and then stirring and chopping them at high speed to form substance B;

[0010] S3, stirring the processed fish meat at high speed to uniformly form substance A, and mixing substance B with substance A;

[0011] S4, add 1-10 parts of alkaline protease and 2-8 parts of neutral protease, and then mix them together in a ratio of 6:4, and then add 5-15 parts of HPRT enzyme and LDH enzyme, the temperature is 30-70°C, the pH value is controlled at 7-8 and 6-7 respectively, and the time is 2-3h;

[0012] S5, centrifuging the mixture from the previous step at low temperature for 10-30 min to obtain a protease mixture;

[0013] S6, filtering and concentrating the liquid to remove unreacted raw materials and impurities to obtain oligopeptide liquid;

[0014] S7. The liquid is spray-dried into fine droplets through a nozzle, and the droplets are exposed to hot air in a container at 50-70° C. for 5-15 minutes to form a powdered oligopeptide product.

[0015] Preferably, the cleaning process in step S1 is as follows: placing the tuna inside a container, adding water to rinse, the water after rinsing is flowing, collecting the rinsed water for filtering and disinfection, and then entering the upper end of the container again after treatment, so that it can recycle the water to clean the tuna in advance.

[0016] Preferably, after the head, viscera and tail of the tuna are separated in step S2, there are a lot of blood stains on the tuna meat, so the tuna meat is taken out and rotated inside the container to be fully cleaned, and then air-dried after cleaning.

[0017] Preferably, an ultrafiltration membrane and a multi-layer filter screen structure are used during filtration in step S6.

[0018] (III) Beneficial effects

[0019] The present invention provides a method for preparing a marine oligopeptide having uric acid lowering activity.

[0020] Beneficial effects:

[0021] The impurities on the outside of fresh tuna are cleaned in advance, and the cleaned wastewater is recycled. The tuna meat, head, viscera, and tail are processed separately and chopped separately to prevent a lot of impurities from affecting the preparation of oligopeptides. HPRT enzyme and LDH enzyme are added, and protease is used in combination to reduce uric acid activity. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] Embodiment 1:

[0024] The present invention provides a method for preparing a marine oligopeptide having uric acid-lowering activity, comprising the following preparation steps:

[0025] S1. Raw material processing: Select fresh tuna, first use clean water to roughly clean the mud and sand stains on its surface, and use a scraper to scrape off the tightly bound impurities, and then use a special knife to scrape off the scales;

[0026] S2, taking out the head, viscera and tail of the tuna separately, cleaning the fish meat and placing it, rotating the head, viscera and tail of the tuna thoroughly to clean them, disinfecting them, and then stirring and chopping them at high speed to form substance B;

[0027] S3, stirring the processed fish meat at high speed to uniformly form substance A, and mixing substance B with substance A;

[0028] S4, add 3 parts of alkaline protease and 3 parts of neutral protease, and then mix them together in a ratio of 6:4, and then add 8 parts of HPRT enzyme and LDH enzyme, the temperature is 40°C, the pH value is controlled at 7.2 and 6.4 respectively, and the time is 2.3 hours;

[0029] S5, centrifuging the mixture from the previous step at low temperature for 10 min to obtain a protease mixture;

[0030] S6, filtering and concentrating the liquid to remove unreacted raw materials and impurities to obtain oligopeptide liquid;

[0031] S7. The liquid is spray-dried into fine droplets through a nozzle, and the droplets are exposed to hot air in a container at 50° C. for 7 minutes to form a powdered oligopeptide product.

[0032] The cleaning process in step S1 is as follows: the tuna is placed inside a container and rinsed with water. After rinsing, the water after rinsing is flowing. The rinsed water is collected for filtration and disinfection using a disinfection machine. After treatment, the water enters the upper end of the container again so that it can recycle the water to clean the tuna in advance.

[0033] After the head, viscera and tail of the tuna are separated in step S2, there are a lot of blood stains on the tuna meat, so the tuna meat is taken out and rotated inside the container to be fully cleaned, and then air-dried after cleaning.

[0034] During the filtration in step S6, an ultrafiltration membrane and a multi-layer filter screen structure are used, and the ultrafiltration membrane and the multi-layer filter screen structure enable the filtration to be very thorough.

[0035] Example 2: A method for preparing a marine oligopeptide having uric acid-lowering activity, comprising the following preparation steps:

[0036] S1. Raw material processing: Select fresh tuna, first use clean water to roughly clean the mud and sand stains on its surface, and use a scraper to scrape off the tightly bound impurities, and then use a special knife to scrape off the scales;

[0037] S2, taking out the head, viscera and tail of the tuna separately, cleaning the fish meat and placing it, rotating the head, viscera and tail of the tuna thoroughly to clean them, disinfecting them, and then stirring and chopping them at high speed to form substance B;

[0038] S3, stirring the processed fish meat at high speed to uniformly form substance A, and mixing substance B with substance A;

[0039] S4, add 6 parts of alkaline protease and 5 parts of neutral protease, and then mix them together in a ratio of 6:4, and then add 11 parts of HPRT enzyme and LDH enzyme, the temperature is 50°C, the pH value is controlled at 7.5 and 6.6 respectively, and the time is 2.6 hours;

[0040] S5, centrifuging the mixture from the previous step at low temperature for 20 minutes to obtain a protease mixture;

[0041] S6, filtering and concentrating the liquid to remove unreacted raw materials and impurities to obtain oligopeptide liquid;

[0042] S7. Spray dry the above liquid into fine droplets through a nozzle, and place them in contact with hot air in a container at 60°C for 10 minutes to form a powdered oligopeptide product:

[0043] Example 3: A method for preparing a marine oligopeptide having uric acid-lowering activity, comprising the following preparation steps:

[0044] S1. Raw material processing: Select fresh tuna, first use clean water to roughly clean the mud and sand stains on its surface, and use a scraper to scrape off the tightly bound impurities, and then use a special knife to scrape off the scales;

[0045] S2, taking out the head, viscera and tail of the tuna separately, cleaning the fish meat and placing it, rotating the head, viscera and tail of the tuna thoroughly to clean them, disinfecting them, and then stirring and chopping them at high speed to form substance B;

[0046] S3, stirring the processed fish meat at high speed to uniformly form substance A, and mixing substance B with substance A;

[0047] S4, add 9 parts of alkaline protease and 7 parts of neutral protease, and then mix them together in a ratio of 6:4, and then add 14 parts of HPRT enzyme and LDH enzyme, the temperature is 60°C, the pH value is controlled at 7.8 and 6.8 respectively, and the time is 2.9 hours;

[0048] S5, centrifuging the mixture from the previous step at low temperature for 30 minutes to obtain a protease mixture;

[0049] S6, filtering and concentrating the liquid to remove unreacted raw materials and impurities to obtain oligopeptide liquid;

[0050] S7. Spray dry the above liquid into fine droplets through a nozzle, and place them in contact with hot air in a container at 70°C for 13 minutes to form a powdered oligopeptide product:

[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a marine oligopeptide having uric acid-lowering activity, characterized in that: The method comprises the following preparation steps: S1. Raw material processing: Select fresh tuna, first use clean water to roughly clean the mud and sand stains on its surface, and use a scraper to scrape off the tightly bound impurities, and then use a special knife to scrape off the scales; S2, taking out the head, viscera and tail of the tuna separately, cleaning the fish meat and placing it, rotating the head, viscera and tail of the tuna thoroughly to clean them, disinfecting them, and then stirring and chopping them at high speed to form substance B; S3, stirring the processed fish meat at high speed to uniformly form substance A, and mixing substance B with substance A; S4, add 1-10 parts of alkaline protease and 2-8 parts of neutral protease, and then mix them together in a ratio of 6:4, and then add 5-15 parts of HPRT enzyme and LDH enzyme, the temperature is 30-70°C, the pH value is controlled at 7-8 and 6-7 respectively, and the time is 2-3h; S5, centrifuging the mixture from the previous step at low temperature for 10-30 min to obtain a protease mixture; S6, filtering and concentrating the liquid to remove unreacted raw materials and impurities to obtain oligopeptide liquid; S7. The liquid is spray-dried into fine droplets through a nozzle, and the droplets are exposed to hot air in a container at 50-70° C. for 5-15 minutes to form a powdered oligopeptide product.

2. The method for preparing a marine oligopeptide having uric acid-lowering activity according to claim 1, characterized in that: The cleaning process in step S1 is as follows: placing the tuna inside the container, adding water to rinse, the water after rinsing is flowing, collecting the rinsed water for filtering and disinfection, and then entering the upper end of the container again after treatment, so that it can recycle the water to clean the tuna in advance.

3. The method for preparing a marine oligopeptide having uric acid-lowering activity according to claim 1, characterized in that: After the head, viscera and tail of the tuna are separated in step S2, there are a lot of blood stains on the tuna meat, so the tuna meat is taken out and rotated inside the container to be fully cleaned, and then air-dried after cleaning.

4. The method for preparing a marine oligopeptide having uric acid-lowering activity according to claim 1, characterized in that: During the filtration in step S6, an ultrafiltration membrane and a multi-layer filter screen structure are used.