Antibacterial and anti-allergy fabric as well as preparation method and application thereof

Abstract

The invention discloses an antibacterial and anti-allergy fabric as well as a preparation method and application thereof, and belongs to the technical field of functional materials and functional textiles. According to the invention, bacillus subtilis subspecies capable of efficiently metabolizing protease is used as a core material, sodium alginate and sodium alginate-chitosan are used as secondary outer layer wall materials, polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA) are used as outer layer wall materials, the core material is embedded, and then the core material is finished on the surface of the fabric, so that the fabric is obtained. The antibacterial and anti-allergy fabric with the characteristics of efficiently decomposing allergens and actively resisting bacteria is prepared; when allergens are adsorbed on the surface of the fabric, metabolites of probiotics can perform targeted decomposition on sensitization sites, meanwhile, adhesion of pathogenic bacteria is reduced, the bacteria are actively killed, and long-term efficient synergism of allergen resistance and bacteria resistance is achieved.

Description

Technical Field

[0001] The invention relates to an antibacterial and anti-allergic fabric and a preparation method and application thereof, belonging to the technical field of functional materials and functional textiles. Background Art

[0002] As living standards continue to improve, people pay more attention to their living environment, hygiene and health care, and functional textiles are becoming more and more popular. Antibacterial and anti-allergic fabrics are closely related to people's lives and have become a key focus.

[0003] At present, the preparation methods of conventional antibacterial and anti-allergic fabrics include dip coating, graft cross-linking, co-spinning, in-situ growth and other methods. However, the dip coating method generally involves finishing antibacterial and anti-allergic agents on fibers, yarns, fabrics, garments or various textile products; but the antibacterial agents only exist on the surface of fibers and fabrics, and the antibacterial effect will be greatly reduced after multiple washings. The graft cross-linking method is through chemical reactions, which is complicated to operate and has high costs. The co-spinning method is to add antibacterial agents and antiallergic agents to the fiber matrix by blending, solution dispersion or masterbatch addition. The antibacterial effect is good and durable, and it is not easy to fall off and lose during post-processing and use, but the preparation technology is difficult, especially the antibacterial agent has high requirements. In-situ growth is to directly reduce metal nano ions on the matrix, focusing on the method of in-situ synthesis of metal nanoparticles, and the operation requirements are high. Therefore, finding an antibacterial and anti-allergic fabric with a simple preparation method and good durability is an urgent problem to be solved.

[0004] Moreover, probiotics and their metabolites can damage the genetic material or important substitution pathways inside pathogenic bacteria, exerting excellent antibacterial functions; as natural biological antibacterial agents, probiotics have good biocompatibility and can also improve the body's immune function, making them an ideal alternative to current chemical antibacterial agents. However, the biggest disadvantage of probiotics is that they have a short viable life and are greatly affected by the colonization environment. Maintaining their long-term survival rate and biological activity is still a challenge facing the probiotic industry. Therefore, it is urgent to find a long-term exogenous material that blocks allergens and has antibacterial properties.

[0005] Microencapsulation technology is the use of natural or synthetic polymer materials to encapsulate solid, liquid or gaseous micro-capsule core materials to form a microcapsule with a semipermeable or sealed capsule membrane. The protection of probiotics by microencapsulation technology is a hot topic of research at home and abroad, and is considered to be one of the most efficient and economical methods to protect the activity of probiotics. It can protect the bacteria and reduce damage to the external environment, improve the survival rate of probiotics during production, processing and storage, and achieve controlled release of probiotics. For example: CN 118511879 A discloses a method for preparing probiotic antibacterial microcapsules, which uses an emulsification method to encapsulate freeze-dried probiotic powder in aminated lignin to obtain probiotic antibacterial microcapsules. However, the copper ions complexed on the surface of the microcapsules have the risk of inducing protein toxic stress and leading to cell death, and the cytotoxicity and safety have not been investigated. CN117926592A discloses a probiotic non-woven fabric and its preparation method and application. The non-woven fabric is prepared by combining polyvinyl alcohol with ethyl orthosilicate under the action of a catalyst to form a copolymer system with polydimethylsiloxane. The copolymer system can form an interpenetrating network system with the polymer material, thereby forming a good bonding effect between the wall material structure of the probiotic microcapsule and the non-woven fabric. However, the modification method of the wall material is complicated and the durability is poor.

[0006] In addition, the application of probiotic microcapsules in the field of textiles is still in its infancy, and the following technical issues need to be improved: first, to improve the biocompatibility of microcapsule wall materials and probiotic core materials to increase the activity of probiotics; second, to improve the affinity of microcapsules to fabric fibers to avoid the use of large amounts of adhesives to adversely affect the physical and chemical properties and comfort of fibers. Summary of the invention

[0007] [Technical issues]

[0008] The preparation of antibacterial and anti-allergic fabrics has problems such as short-lasting antibacterial and anti-allergic effects, complicated operation, and poor durability;

[0009] The preparation of probiotic microcapsules has problems such as harmful substances, complex modification and poor adhesion performance.

[0010] [Technical solution]

[0011] In order to solve the above problems, the present invention provides an antibacterial and anti-allergic fabric and its preparation method and application. The antibacterial and anti-allergic fabric prepared by the present invention can achieve the effect of exogenous adsorption and decomposition of allergens and long-term antibacterial properties, avoiding the harm of external allergens and harmful bacteria to the human body.

[0012] The first object of the present invention is to provide a method for preparing antibacterial and anti-allergic probiotic microcapsules, comprising the following steps:

[0013] (1) washing and resuspending the activated probiotic bacterial liquid to obtain a probiotic resuspension;

[0014] (2) adding the secondary outer wall material solution to the probiotic resuspension, mixing to form an aqueous phase; adding the oil phase to the aqueous phase, stirring, adding a crosslinking agent, stirring, centrifuging, washing, and obtaining single-wall microcapsules; wherein the secondary outer wall material is one or both of sodium alginate and sodium alginate-chitosan;

[0015] (3) Dispersing the single-wall microcapsules in water, adding the outer wall material solution, rotary evaporating, centrifuging, washing, and freeze-drying to obtain double-wall microcapsules; wherein the outer wall material is one or both of polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA).

[0016] In one embodiment of the present invention, the probiotic in step (1) is Bacillus subtilis subsp. subtilis BNCC132861.

[0017] In one embodiment of the present invention, prebiotics can be added to the probiotic resuspension in step (1), and the prebiotics are one or more of inulin, fucoidan, and α-lactalbumin. The dosage ratio of prebiotics to probiotic resuspension is 1-3 g:100 mL.

[0018] In one embodiment of the present invention, the method for preparing the activated probiotic solution in step (1) is as follows:

[0019] The frozen probiotic strains were activated at 30-37°C for 12-24 hours; then placed in beef extract peptone medium (BPM), cultured at 30-37°C, 50-200 rpm for 12-24 hours, with an inoculation amount of 0.5-1.5% (v / v).

[0020] In one embodiment of the present invention, the activated probiotic bacterial solution in step (1) is washed and resuspended using a PBS solution.

[0021] In one embodiment of the present invention, the concentration of probiotics in the probiotic resuspension of step (1) is 10 8 -10 10 CFU / mL.

[0022] In one embodiment of the present invention, the secondary outer wall material solution in step (2) is a secondary outer wall material aqueous solution with a mass concentration of 1-3wt%, and the outer wall material solution needs to be sterilized under the conditions of: sterilization at 121°C for 20 minutes.

[0023] In one embodiment of the present invention, the volume ratio of the probiotic resuspension to the secondary outer wall material solution in step (2) is 1:100-10:100.

[0024] In one embodiment of the present invention, the oil phase in step (2) is a mixture of vegetable oil and Tween 80, the vegetable oil includes one or more of soybean oil, coconut oil, and palm oil; the mass ratio of vegetable oil to Tween 80 is 100:0.5-2.5.

[0025] In one embodiment of the present invention, in step (2), the volume ratio of the water phase, the oil phase, and the cross-linking agent is 1:2:1-1:3:1.

[0026] In one embodiment of the present invention, the cross-linking agent in step (2) is CaCl 2 Aqueous solution with a concentration of 0.05-0.5M.

[0027] In one embodiment of the present invention, the stirring in step (2) is carried out at 20-30° C. and 400-1200 rpm for 30-70 min.

[0028] In one embodiment of the present invention, the centrifugation and washing in step (2) is performed at 3000-5000 rpm for 5-15 min, the upper oil phase and the lower water phase are discarded, and washing is performed with water.

[0029] In one embodiment of the present invention, the solvent of the outer wall material solution in step (3) is ethyl acetate with a concentration of 3-5 wt%.

[0030] In one embodiment of the present invention, in step (3), the ratio of single-walled microcapsules, water, and outer wall material solution is 1 g: 8-12 mL: 100 mL, more preferably 1 g: 10 mL: 100 mL.

[0031] In one embodiment of the present invention, the rotary evaporation in step (3) is carried out at 35-45° C. and a vacuum degree of 400-600 mbar for 10-45 min.

[0032] In one embodiment of the present invention, the centrifugation and washing in step (3) are performed at 3000-5000 rpm for 5-15 min, the clear liquid is discarded, and washing is performed with water.

[0033] In one embodiment of the present invention, the freeze-drying in step (3) is performed at -20 to -40°C for 24 to 48 hours.

[0034] The second object of the present invention is the antibacterial and anti-allergic probiotic microcapsules prepared by the method of the present invention.

[0035] The third object of the present invention is to provide a method for preparing antibacterial and anti-allergic fabrics, comprising the following steps:

[0036] The unsizing cotton fabric is placed in an alkali solution, boiled, degreased and impurities removed, and surface hydroxyl groups are introduced to obtain a pretreated cotton fabric;

[0037] Dispersing antibacterial and anti-allergic probiotic microcapsules in a cationic solution, adding a cross-linking agent to obtain a cross-linking agent solution;

[0038] The pretreated cotton fabric is placed in a crosslinking agent solution for crosslinking; after the crosslinking is completed, the fabric is washed and dried to obtain an antibacterial and anti-allergic fabric.

[0039] In one embodiment of the present invention, the alkaline solution is a NaOH aqueous solution with a mass concentration of 1-3%; and the boiling is performed at 85-95° C. for 20-40 minutes.

[0040] In one embodiment of the present invention, the cationic solution is a polydiallyldimethylammonium chloride aqueous solution with a mass fraction of 0.1-0.5 wt %.

[0041] In one embodiment of the present invention, the cross-linking agent is citric acid, and the mass fraction of the cross-linking agent in the cationic solution is 0.5-1%.

[0042] In one embodiment of the present invention, the mass ratio of the antibacterial and anti-allergic probiotic microcapsules to the cationic solution is 1-2:100.

[0043] In one embodiment of the present invention, the mass ratio of the pretreated cotton fabric to the cross-linking agent solution is 1:10-100.

[0044] In one embodiment of the present invention, crosslinking is performed at 20-30° C. (room temperature) for 20-40 min, which may be repeated 1-3 times.

[0045] In one embodiment of the present invention, washing is performed with water.

[0046] In one embodiment of the present invention, the drying is oven drying at 30-45°C.

[0047] The fourth object of the present invention is the antibacterial and anti-allergic fabric prepared by the method of the present invention.

[0048] The fifth object of the present invention is to provide a method for improving the antibacterial and anti-allergic properties of cotton fabrics, which uses the antibacterial and anti-allergic fabrics described in the present invention.

[0049] The sixth object of the present invention is to provide a clothing fabric, which adopts the antibacterial and anti-allergic fabric described in the present invention.

[0050] The seventh object of the present invention is the application of the antibacterial and anti-allergic fabric of the present invention in textiles for clothing.

[0051] [Beneficial Effects]

[0052] (1) The method for preparing antibacterial and anti-allergic fabrics described in the present invention has a short process flow and high production efficiency, and avoids the negative impact of inorganic and organic antibacterial finishing agents on the balance of human flora.

[0053] (2) The outer wall material of the microcapsule used in the present invention is self-degradable, and its degradation products can inhibit the proliferation of harmful bacteria by adjusting the pH; the secondary outer layer of the microcapsule effectively takes into account the primary brittleness and secondary toughness by adjusting the degree of cross-linking, while providing long-term protection for the core material. The probiotics in the microcapsules on the surface of the fabric can be released by friction or washing, and colonized on the surface of the fabric, achieving a long-term antibacterial and anti-allergic effect.

[0054] (3) The present invention uses a subspecies of Bacillus subtilis that can efficiently metabolize proteases as the core material, sodium alginate and sodium alginate-chitosan as the secondary outer wall material, and polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA) as the outer wall material to embed the core material, and then arrange it on the surface of the fabric to prepare an antibacterial and anti-allergic fabric with efficient allergen decomposition and active antibacterial properties; when the allergens are adsorbed on the surface of the fabric, the metabolites of the probiotics can be targeted and decomposed at the sensitizing sites, while reducing the adhesion of pathogenic bacteria and actively killing bacteria, thereby achieving long-term and efficient synergy of anti-allergy and antibacterial properties. DETAILED DESCRIPTION

[0055] The preferred embodiments of the present invention are described below. It should be understood that the embodiments are for better explaining the present invention and are not used to limit the present invention.

[0056] Test method:

[0057] 1. Test of microcapsule yield and embedding rate:

[0058]

[0059] 2. Air permeability test:

[0060] GB / T 5453-1997 “Determination of air permeability of textile fabrics”.

[0061] 3. Mechanical properties test:

[0062] GBT3923.1-2013 "Tensile properties of textile fabrics - Part 1: Determination of breaking strength and elongation at break - Strip method".

[0063] 4. Antibacterial performance test:

[0064] GB / T 20944.3-2008 “Evaluation of antimicrobial properties of textiles Part 3: Oscillation method”.

[0065] 5. Anti-allergic performance test:

[0066] ELISA:

[0067] The commercially available der f 1 kit (inbio) was used for testing. A 5×5 cm fabric sample was placed in a sealed bag, 1 mL of test solution was added, and the sample was squeezed out after rubbing. 100 μL was added to each test well, and the test solution that did not react with the fabric was added to the control well. The sample was incubated at room temperature at 300 rpm for 1 h, the liquid was discarded, the wells were washed, a mixture of secondary antibody and marker was added, and the sample was incubated at room temperature at 300 rpm for 1 h in the dark. 100 μL of TMB solution was added to each well, and the color was developed for 10-25 min. The stop solution was added, the absorbance was measured, and the amount of antigen in each well was determined based on the standard curve.

[0068] The calculation formula of anti-allergy is as follows:

[0069]

[0070] The raw materials used in the examples are:

[0071] Polyhydroxyalkanoate PHA:RG, CAS:26744-04-7, purchased from adamas.

[0072] Poly(lactic-co-glycolic acid) PLGA: CAS: 30846-39-0, purchased from Macklin.

[0073] Sodium alginate: RG, CAS: 9005-38-3, purchased from adamas.

[0074] Chitosan: RG, CAS: 9012-76-4, purchased from adamas.

[0075] Preparation method of sodium alginate-chitosan solution:

[0076] A 2 wt % sodium alginate aqueous solution and a 2 wt % chitosan aqueous solution were stirred and mixed at a volume ratio of 1:1 to obtain a uniform composite solution, which was sterilized at 121° C. for 20 min, and then cooled to room temperature for standby use.

[0077] Inulin: BC, CAS: 9005-80-5, purchased from Aladdin.

[0078] Fucoidan: RG, CAS: 9072-19-9, purchased from adamas.

[0079] Bacillus subtilis: Bacillus subtilis subsp. subtilis BNCC132861.

[0080] Beef extract peptone medium (BPM): purchased from Qingdao Hibo Biotechnology.

[0081] Preparation method of activated probiotic solution:

[0082] The frozen probiotic strain (Bacillus subtilis subsp. subtilis BNCC132861) was activated at 37°C for 24 hours; then placed in beef extract peptone medium (BPM), cultured at 37°C and 120 rpm for 24 hours, with an inoculation amount of 1% (v / v).

[0083] PBS solution: purchased from Beekman Biotechnology Co., Ltd.

[0084] Water: deionized water.

[0085] Unslurried cotton fabric: GB / T8629-2017 standard pure cotton fabric, 378g / m 2 , purchased from Ningbo Textile Consumables Instrument Center.

[0086] The solutions mentioned in the examples and comparative examples without specifying the solvent are water, the % mentioned without specifying the meaning refers to the mass percentage, and the reaction conditions mentioned without specifying the temperature refer to the room temperature of 20-30°C.

[0087] Example 1

[0088] A method for preparing antibacterial and anti-allergic probiotic microcapsules comprises the following steps:

[0089] (1) The activated probiotic bacterial solution was washed once with a PBS solution, and resuspended with a PBS solution to obtain a probiotic resuspension; wherein the concentration of the probiotics in the probiotic resuspension was 10 10 CFU / mL;

[0090] (2) mixing sodium alginate and water evenly, sterilizing at 121° C. for 20 min, and cooling to room temperature to obtain a sodium alginate aqueous solution with a mass fraction of 2%;

[0091] Mix soybean oil and Tween 80 in a mass ratio of 100:1 to form an oil phase;

[0092] Inulin is added to the probiotic resuspension and mixed evenly, and then added to a 2% sodium alginate aqueous solution and mixed evenly to form an aqueous phase; wherein the amount ratio of the probiotic resuspension, inulin and sodium alginate aqueous solution is 5 mL: 3 g: 100 mL;

[0093] The oil phase was added to the aqueous phase, stirred at 25°C and 600 rpm for 30 min, and 0.2 M CaCl 2 The aqueous solution was stirred at 25°C and 600 rpm for 30 min, centrifuged at 5000 rpm for 10 min, the upper oil phase and the lower water phase were discarded, and washed with water to obtain single-wall microcapsules; wherein the water phase, oil phase, CaCl 2The volume ratio of the aqueous solution is 1:2:1;

[0094] (3) mixing polyhydroxyalkanoate PHA and ethyl acetate uniformly to obtain a polyhydroxyalkanoate PHA solution with a mass fraction of 2 wt%;

[0095] The single-walled microcapsules were dispersed in water, and polyhydroxyalkanoate PHA solution was added. The mixture was rotary evaporated at 40°C and vacuum degree of 500 mbar for 20 min, and centrifuged at 5000 rpm for 10 min. The supernatant was discarded, and the mixture was washed with water. The mixture was freeze-dried at -20°C for 48 h to obtain double-walled microcapsules. The ratio of single-walled microcapsules, water, and polyhydroxyalkanoate PHA solution was 1 g: 10 mL: 100 mL.

[0096] Example 2

[0097] In step (2) of Example 1, the inulin is changed to fucoidan, the sodium alginate aqueous solution with a mass fraction of 2 wt% is changed to a sodium alginate-chitosan solution; the polyhydroxyalkanoate PHA solution with a mass fraction of 3 wt% in step (3) is changed to a PHA / PLGA (mass ratio 1:1) solution with a total mass fraction of 2 wt%;

[0098] Other steps were the same as those in Example 1 to obtain double-wall microcapsules.

[0099] Example 3

[0100] Adjust the CaCl in step (2) of Example 1 2 The concentration of the aqueous solution was 0.05 M, and the other conditions were the same as those in Example 1, to obtain double-walled microcapsules.

[0101] Example 4

[0102] Adjust the CaCl in step (2) of Example 2 2 The concentration of the aqueous solution was 0.05 M, and the other conditions were the same as those in Example 2, to obtain double-walled microcapsules.

[0103] Example 5

[0104] The polyhydroxyalkanoate PHA solution with a mass fraction of 3 wt % in step (3) of Example 1 was adjusted to a PLGA solution with a mass fraction of 2 wt %; the rest was kept consistent with Example 1 to obtain double-walled microcapsules.

[0105] Example 6

[0106] The sodium alginate aqueous solution with a mass fraction of 2 wt % in step (2) of Example 1 was adjusted to a sodium alginate-chitosan solution; the rest was kept consistent with Example 1 to obtain double-walled microcapsules.

[0107] Example 7

[0108] The inulin in step (2) of Example 1 was omitted, and the other steps were the same as in Example 1 to obtain double-walled microcapsules.

[0109] Comparative Example 1

[0110] Step (3) of Example 1 was omitted, and the rest of the steps were the same as those of Example 1 to obtain single-wall microcapsules.

[0111] Comparative Example 2

[0112] Step (3) of Example 2 was omitted, and the rest of the steps were the same as in Example 2 to obtain single-wall microcapsules.

[0113] Comparative Example 3

[0114] The sodium alginate in step (2) of Example 1 was adjusted to gelatin, and the other steps were kept the same as in Example 1 to obtain double-walled microcapsules.

[0115] Comparative Example 4

[0116] Step (3) of Example 1 was omitted, and the sodium alginate aqueous solution in step (2) was replaced by a polyhydroxyalkanoate (PHA) solution. The other steps were the same as those of Example 1 to obtain single-wall microcapsules.

[0117] The microcapsules obtained in the examples and comparative examples were subjected to performance tests, and the test results are as follows:

[0118] Table 1

[0119] example Yield (%) Embedding rate (%) Example 1 72.67 74.23 Example 2 68.85 73.47 Example 3 69.70 70.95 Example 4 62.68 69.60 Example 5 71.56 73.17 Example 6 70.21 72.04 Example 7 65.81 67.95 Comparative Example 1 75.78 71.05 Comparative Example 2 71.74 72.59 Comparative Example 3 68.33 63.14 Comparative Example 4 72.27 63.17

[0120] From Table 1 we can see that:

[0121] (1) The yield of single-walled microcapsules is slightly higher than that of double-walled microcapsules. This is because there is a certain loss in the process of coating the outer wall material.

[0122] (2) The yield and embedding rate of double-walled microcapsules with low cross-linking degree are lower than those of other double-walled microcapsules. This is because the low cross-linking degree makes the microcapsules coated with only the secondary outer wall material prone to swelling and rupture during the centrifugation, washing, and secondary coating process.

[0123] (3) The yield and encapsulation rate of samples without prebiotics were low. The reason is that after adding prebiotics, probiotics can be adsorbed on the surface of prebiotics and are easier to encapsulate.

[0124] Example 8

[0125] A method for preparing antibacterial and anti-allergic fabric comprises the following steps:

[0126] The unsizing cotton fabric is placed in a 2% NaOH aqueous solution, boiled at 90°C for 30 minutes, degreased and impurities removed, and surface hydroxyl groups are introduced to obtain a pretreated cotton fabric;

[0127] The microcapsules prepared in the embodiment and the comparative example are dispersed in a polydiallyldimethylammonium chloride aqueous solution with a mass fraction of 0.5wt%, and citric acid is added to obtain a crosslinking agent solution; wherein the mass fraction of citric acid in the polydiallyldimethylammonium chloride aqueous solution is 1%; the mass ratio of the antibacterial and anti-allergic probiotic microcapsules to the polydiallyldimethylammonium chloride aqueous solution is 2:100;

[0128] The pretreated cotton fabric was placed in a crosslinking agent solution, crosslinked at 25°C for 30 minutes, and repeated twice; after completion, it was washed with water and dried naturally to obtain an antibacterial and anti-allergic fabric, wherein the mass ratio of the pretreated cotton fabric to the crosslinking agent solution was 1:10.

[0129] Comparative Example 5

[0130] A method for preparing antibacterial and anti-allergic fabric comprises the following steps:

[0131] (1) The activated probiotic bacterial solution was washed once with a PBS solution, and resuspended with a PBS solution to obtain a probiotic resuspension; wherein the concentration of the probiotics in the probiotic resuspension was 10 10 CFU / mL;

[0132] (2) placing the unsizing cotton fabric in a 2% NaOH aqueous solution, boiling it at 90° C. for 30 min, degreasing and removing impurities, and introducing surface hydroxyl groups to obtain a pretreated cotton fabric;

[0133] (3) 4 g of pretreated cotton fabric was placed in 50 mL of probiotic heavy suspension, and the fabric was immersed twice and rolled twice, with a rolling rate of 93.31%. The fabric was dried at 60° C. to remove excess water, thereby obtaining a fabric.

[0134] The obtained fabric was subjected to performance tests, and the test results are as follows:

[0135] Table 2

[0136] Microcapsules Air permeability (mm / s) Elongation at break in warp direction (%) Elongation at break in weft direction (%) Unsizing cotton fabric 87.52 18.05 19.53 Example 1 80.61 18.95 19.21 Example 2 81.09 19.03 19.03 Example 3 82.12 19.24 19.37 Example 4 81.27 18.92 18.96 Example 5 80.11 19.31 19.01 Example 6 82.37 18.89 18.47 Example 7 84.91 19.56 18.72 Comparative Example 1 85.04 18.49 18.92 Comparative Example 2 83.21 18.73 18.50 Comparative Example 3 79.87 18.45 18.31 Comparative Example 4 81.01 19.07 18.27 Comparative Example 5 83.07 18.86 18.58

[0137] It can be seen from Table 2 that the air permeability of the fabric decreases slightly after finishing, which is because the yarn gaps are filled with microcapsules after the fabric is finished. The strength of the finished fabric increases in the warp direction, while the strength in the weft direction remains stable and decreases slightly. The increase in the warp elongation at break is due to the fact that after the NaOH treatment, the cellulose microfiber structure inside the cotton fiber is rearranged, the crystalline area is reduced, the amorphous area is increased, the fiber cross-sectional area is increased, the length is slightly shortened, the tension in the yarn is reduced, and the bonding force between the fibers is increased, and the yarn becomes tighter. The change in the weft elongation at break is due to the fact that the weft yarn is subjected to less tension during the weaving process and originally has higher ductility. After the NaOH treatment, the fiber expansion and the rearrangement of the microfiber structure may lead to a decrease in the fiber's ductility. Therefore, the fabric pretreatment time needs to be controlled to maintain its mechanical properties.

[0138] Table 3 Test results of Escherichia coli ATCC8739

[0139]

[0140] Table 4 Test results of Escherichia coli ATCC8739

[0141]

[0142] Table 5 Staphylococcus aureus ATCC6538 test results

[0143]

[0144] Table 6 Staphylococcus aureus ATCC6538 test results

[0145]

[0146] Table 7 Candida albicans ATCC10231 test results

[0147]

[0148] Table 8 Candida albicans ATCC10231 test results

[0149]

[0150] It can be seen from Table 3-Table 8 that the fabrics with added probiotics have good antibacterial properties against Escherichia coli, Staphylococcus aureus, and Candida albicans. The initial antibacterial rate is less than the antibacterial rate of the extended storage time because the probiotics enter a dormant state during the reaction process and increase over time. Since prebiotics are added to the microcapsules, the probiotics recover their activity, thereby increasing the antibacterial rate. Embodiments 3 and 4 reduce the cross-linking degree of the wall material, making it easier to break due to external force, so their antibacterial rates are slightly greater than those of Embodiments 1 and 2. The initial antibacterial rate of Comparative Example 2 is the largest because chitosan is a good antibacterial agent, and its antibacterial effect is better than that of PHA and PLGA. At the same time, with the increase of storage time, probiotics are completely colonized and proliferated on the fabric, so the antibacterial rate of the sample in the embodiment gradually increases, while the fabric of Comparative Example 5 impregnated with pure bacterial solution has no protection for probiotics and no energy supply material, and the number of live bacteria decreases over time, so the antibacterial rate gradually decreases.

[0151] Table 9 ELISA test results

[0152]

[0153] Table 10 ELISA test results

[0154]

[0155] It can be seen from Tables 9 and 10 that with the increase of storage time, the adsorption rate generally increases slightly. Among them, the adsorption of fabrics treated with single-walled microcapsules first increases and then decreases. The reason is that single-walled microcapsules are easier to disintegrate than double-walled microcapsules. Therefore, after the microcapsules disintegrate, the probiotics first colonize on the surface of the fabric, which can achieve the decomposition of allergens. Over time, due to the lack of protection and energy supply of wall materials and prebiotics, the probiotics gradually die, so the adsorption rate decreases.

[0156] Although the present invention has been disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for preparing antibacterial and anti-allergic probiotic microcapsules, characterized in that: The steps include: (1) washing and resuspending the activated probiotic bacterial liquid to obtain a probiotic resuspension; (2) adding the secondary outer wall material solution to the probiotic resuspension, mixing to form an aqueous phase; adding the oil phase to the aqueous phase, stirring, adding a crosslinking agent, stirring, centrifuging, washing, and obtaining single-wall microcapsules; wherein the secondary outer wall material is one or both of sodium alginate and sodium alginate-chitosan; (3) Dispersing the single-wall microcapsules in water, adding the outer wall material solution, rotary evaporating, centrifuging, washing, and freeze-drying to obtain double-wall microcapsules; wherein the outer wall material is one or both of polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA).

2. The method according to claim 1, characterized in that In step (1), the probiotic is Bacillus subtilis.

3. The method according to claim 1, characterized in that In step (2), the volume ratio of the water phase, the oil phase and the cross-linking agent is 1:2:1-1:3:

1.

4. The method according to claim 1, characterized in that: In step (2), the cross-linking agent is a CaCl2 aqueous solution with a concentration of 0.05-0.5M.

5. The antibacterial and anti-allergic probiotic microcapsules prepared according to the method according to any one of claims 1 to 4.

6. A method for preparing antibacterial and anti-allergic fabrics, characterized in that: The steps include: The unsizing cotton fabric is placed in an alkali solution, boiled, degreased and impurities removed, and surface hydroxyl groups are introduced to obtain a pretreated cotton fabric; Dispersing the antibacterial and anti-allergic probiotic microcapsules according to claim 5 in a cationic solution, adding a cross-linking agent to obtain a cross-linking agent solution; The pretreated cotton fabric is placed in a crosslinking agent solution for crosslinking; after the crosslinking is completed, the fabric is washed and dried to obtain an antibacterial and anti-allergic fabric.

7. The antibacterial and anti-allergic fabric prepared by the method according to claim 6.

8. A method for improving the antibacterial and anti-allergic properties of cotton fabrics, characterized in that: The antibacterial and anti-allergic fabric according to claim 7 is used.

9. A clothing fabric, characterized in that: The antibacterial and anti-allergic fabric according to claim 7 is used.

10. Use of the antibacterial and anti-allergic fabric according to claim 7 in textiles for clothing.

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