A multi-chamber multiplexed animal respiration, heat measurement and metabolic analysis system and detection method

Abstract

The present invention discloses a multi-cabin multiplexed animal respiration thermometry metabolic analysis system and a detection method in the field of metabolic cabin technology, comprising a metabolic cabin control group and multiple metabolic living cabins. An equipment cabinet is arranged inside the metabolic cabin control group, and detection pipelines for collecting gas and gas transmission pipelines for conveying gas are respectively arranged between the equipment cabinet and the multiple metabolic living cabins. A buffer empty cabin for conveying gas buffer is arranged on the inner rear side of each metabolic living cabin, and the buffer empty cabin is connected to the gas transmission end of the gas transmission pipeline, an exhaust solenoid valve is installed between two adjacent detection pipelines, and an intake solenoid valve is installed between two adjacent gas transmission pipelines. The present invention utilizes the buffer empty cabin to ensure the stability of the gas entering the metabolic living cabin, and flushes and corrects the sensor inside the concentration analyzer with standard gas, thereby reducing the accuracy of the detection data affected by the internal drift of the sensor inside the concentration analyzer.

Description

Technical Field

[0001] The present invention relates to the technical field of metabolic chambers, and in particular to a multi-chamber multiplexed animal respiration heat measurement metabolic analysis system and a detection method. Background Art

[0002] Metabolomics generates a spectrum of small molecules derived from cellular metabolism that can directly reflect the results of complex biochemical reaction networks, thereby providing insights into multiple aspects of cellular physiology. Its experimental results directly reflect the activity of the metabolic network that leads to the production of these metabolites and produce basic information about the underlying biological state of the system. Therefore, metabolomics is not defined by any specific experiment, but rather reflects the study of metabolism in a comprehensive manner. Therefore, metabolomics testing has become a powerful tool for studying the metabolic regulatory mechanisms of pathophysiology.

[0003] A metabolic chamber is a device that uses indirect calorimetry to measure human metabolic consumption. It measures the oxygen consumption and carbon dioxide generation of the human body in the chamber, and uses the Weir formula to calculate the energy metabolic rate (MR, where MR is the speed at which animals convert food into energy, and is one of the important indicators of physical health) and respiratory entropy (RQ, where RQ can indicate the metabolic results of overnutrition or undernutrition, and is one of the important indicators of physical health). Multi-cabin animal metabolic chambers can be reused in multiple chambers, and multi-cabin metabolic chambers can measure metabolic data for multiple animals through a cyclic measurement method, so that they are suitable for use in animal husbandry. When using a multi-cabin metabolic chamber to detect metabolic data of animals, it is necessary to ensure the stability of the gas environment inside the chamber, because the slow and rapid changes in the gas inside the chamber will cause uneven distribution of CO2 and O2 gases in the gas, thereby affecting the results of subsequent sampling and detection. At the same time, the CO2 and O2 sensors of the gas analyzer drift after working for a long time, and need to be calibrated by introducing standard gas. Based on this, the present invention designs a multi-cabin multiplexed animal respiratory calorimetry metabolic analysis system and a method for detecting metabolic data to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide a multi-chamber multiplexed animal respiration thermal metabolism analysis system and detection method to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A multi-cabin multiplexed animal respiration heat measurement metabolic analysis system comprises a metabolic cabin control group and multiple metabolic living cabins, the metabolic cabin control group comprises a control room and an assembly wall, the assembly wall and the metabolic living cabin are spliced ​​and assembled by a mortise and tenon structure, the equipment cabinet is arranged inside the control room, multiple metabolic living cabins are installed on one side of the metabolic cabin control group, an equipment cabinet for accommodating detection equipment and gas transmission equipment is arranged inside the metabolic cabin control group, and detection pipelines for collecting gas and transmission pipelines for transmitting gas are respectively arranged between the equipment cabinet and the multiple metabolic living cabins. The gas pipeline, the inner rear side of each group of metabolic living cabins is provided with a buffer cabin for conveying gas buffer, and the buffer cabin is connected with the gas supply end of the gas supply pipeline, an exhaust solenoid valve is installed between two adjacent groups of detection pipelines, and an intake solenoid valve is installed between two adjacent groups of gas supply pipelines, the buffer cabin includes a cabin connected with the input end of the gas supply pipeline, the inner cavity of the cabin is provided with an arc-shaped diverter plate, and the convex surface of the diverter plate is arranged below the output end of the gas supply pipeline in the inner cavity of the cabin, and the front side of the cabin is provided with a shutter connected with the inner cavity of the metabolic living cabin.

[0007] Preferably, the equipment cabinet is designed with front and rear double chambers, the front chamber of the equipment cabinet is provided with a drying chamber, an EPC, a concentration analyzer and a control host in order from top to bottom, the rear chamber of the equipment cabinet is provided with an air intake fan, an exhaust fan, a standard gas cylinder group and a ruby ​​air resistance, and a computer is provided inside the control chamber;

[0008] The drying chamber is used to detect the drying of the gas, the EPC is used to control the pressure input into the detection equipment, the concentration analyzer is used to analyze and detect the gas concentration after drying, the control host is used to receive the detection data of the concentration analyzer, output the detection data, accept and execute control commands, the air intake fan is used to transport the external gas into the metabolic living cabin, the exhaust fan is used to extract the gas inside the metabolic living cabin for detection, the standard gas cylinder group is used to provide standard gas for flushing and correction, the proportions of nitrogen, oxygen and carbon dioxide in the standard gas inside the standard gas cylinder group are 79%, 21% and 0%, the ruby ​​gas resistance is used to limit the flow rate of the outflowing detection gas, and the computer is used to receive control host data and send control commands to the control host.

[0009] Preferably, the output end of the standard gas cylinder group is connected to the output end of the exhaust fan through an air supply solenoid valve, the input end of the exhaust fan is connected to one end of the detection pipeline, and the detection pipeline passes through the partition between the front and rear chambers of the equipment cabinet, and the ruby ​​gas resistor is arranged between the output end of the gas supply solenoid valve and the input end of the drying chamber, and the other end of the ruby ​​gas resistor is connected to the input end of the drying chamber through a pipeline passing through the partition between the front and rear chambers of the equipment cabinet.

[0010] Preferably, the drying chamber is a resistance wire heating drying tube, the EPC is an electronic pressure controller, the concentration analyzer is a URAS26 type infrared sensor and a differential pressure paramagnetic oxygen sensor analyzer, the air intake fan and the exhaust fan are both V DC brushless fans, the control host communicates with the concentration analyzer through an interface, and the computer and the control host communicate through a wireless network.

[0011] On the other hand, the present invention also discloses a detection method of a multi-cabin multiplexed animal respiration thermal metabolism analysis system, the specific steps of which are as follows:

[0012] S1, empty cabin debugging: send a command to control the start of the air intake fan and the exhaust fan to the control host through the computer, so that the external gas can be transported to the buffer empty cabin through the air intake fan, and the gas can be stably transported to the metabolic living cabin after the diversion and buffering of the buffer empty cabin. Then send a command to the control host through the computer to synchronously open the air intake solenoid valve 1 and the exhaust solenoid valve 1, and the air intake solenoid valves and exhaust solenoid valves of other groups are in a closed state, so that the gas can pass through the interior of the first group of metabolic living cabins, but cannot enter the interior of other metabolic living cabins. Apply soapy water at the connection ends of each pipeline to determine whether there is a leak;

[0013] After eliminating the pipeline leakage, observe the fluctuation of the data detected by the concentration analyzer recorded in the computer. When the detected data no longer fluctuates greatly, it means that the electrical equipment can operate normally and stably. When the detected data keeps fluctuating greatly, it means that the electrical equipment has abnormal operation and needs to be eliminated. In this way, only the air intake solenoid valve and the exhaust solenoid valve on the same metabolic living cabin are opened one by one, and the air intake solenoid valves and the exhaust solenoid valves on the other metabolic living cabins are in a closed state. The pipeline leakage and abnormal operation of the electrical equipment can be eliminated for each metabolic living cabin in turn.

[0014] S2, data initialization: In the step of eliminating the abnormal electrical operation of the equipment in step S1, the judgment is made by observing the fluctuation size of the detection data. On the basis of this step, the gas transmission solenoid valve is controlled by a computer to connect the output end of the standard gas cylinder group with the input end of the concentration analyzer, so that the standard gas in the standard gas cylinder group can flush and calibrate the sensor inside the concentration analyzer. The concentration analyzer is flushed with the standard gas, and the data drift of each detection data point and the standard gas is obtained in the computer using the differential function formula. When the drift is less than 1%, it means that the internal sensor of the concentration analyzer is in the initial calibration state. Otherwise, it means that the internal sensor of the concentration analyzer is in an unstable state, and the concentration analyzer needs to be continuously flushed with standard gas until its internal sensor is in the initial calibration state. At the same time, the time period t0 from flushing to calibration completion is recorded;

[0015] S3, metabolic data detection: after the internal sensor of the concentration analyzer is in the initial calibration state in step S2, the experimental subject that needs to be detected by metabolic data is sent to the metabolic living cabin for metabolic data detection. Before entering the metabolic living cabin, the experimental subject needs to be uniformly cleaned and disinfected to reduce the impact of external microorganisms on the consumption of gas inside the metabolic living cabin;

[0016] Then, the air intake rate V of the air intake fan is set by the computer. 进气速率 is a fixed value, and the exhaust gas rate V of the exhaust fan is set 抽气速率 is a fixed value, and the gas velocity V entering the concentration analyzer under the ruby ​​gas resistance limit 检测速率 is a fixed value, the drying temperature of the drying chamber is T, the pressure data of EPC is Pa, and since the gas entering the metabolic living cabin is the outside atmosphere, the gas concentration of CO2 entering is PCO 2进气 and O2 gas concentration PO 2进气 is a determined value;

[0017] At the same time, the cycle t of the concentration analyzer for collecting and detecting data is set through the computer, and the cycle of standard gas flushing after the data collection cycle t is the time cycle t0 measured in step S2. The data collection of each metabolic living cabin in each cycle t is independent, that is, when the metabolic living cabin of this group collects data in the cycle t, the air inlet solenoid valve and the exhaust solenoid valve of other metabolic living cabins are in a closed state, and only the gas in the metabolic living cabin can be collected and detected. After the collected gas is dried in the drying chamber, it is released by the EPC and enters the concentration analyzer to detect the CO2 concentration data and the O2 concentration data, and the PCO2 and PO2 data are obtained. The detected PCO2 and PO2 data are transmitted to the computer through the control host;

[0018] S4, calculation of metabolic data: According to the CO2 concentration data PCO2 and O2 concentration data PCO2 detected in step S3, the CO2 change rate VCO2 and O2 change rate VO2 of the experimental subject in the metabolic living chamber are calculated in the computer, and the calculation formula is as follows:

[0019] VCO2=(PCO2-PCO 2进气 )×(V 进气速率 -V 检测速率 )

[0020] VO2=(PO 2进气 -PO2)×(V 进气速率 -V 检测速率 )

[0021] According to the calculated CO2 change rate VCO2 and O2 change rate VO2 data, the metabolic rate MR and respiratory entropy RQ of the experimental subject in the metabolic living chamber are further calculated, and the metabolic rate MR of the experimental subject is calculated by the Weir equation, as follows

[0022] MR=(k1×VCO2)+(k2×VO2)

[0023] Among them, k1 is the metabolic coefficient of the experimental subject's respiratory metabolism to produce CO2, and k2 is the metabolic coefficient of the experimental subject's respiratory metabolism to consume O2;

[0024] The calculation formula of respiratory entropy RQ is:

[0025]

[0026] Based on the calculated metabolic rate MR data and respiratory entropy RQ data, combined with the period t during the detection process, the MR-t curve and RQ-t curve are drawn on the computer using the Smooth function.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The present invention utilizes the arc-shaped design of the diverter plate to buffer and divert the gas entering the cabin through the output end of the gas transmission pipeline, so that the gas can smoothly enter the interior of the metabolic living cabin, thereby reducing the phenomenon of uneven distribution of the internal environmental gas caused by the input gas being directly discharged into the interior of the metabolic living cabin, thereby reducing the phenomenon of large fluctuations in the concentration of the detected gas caused by the rapid changes in the internal environmental gas of the metabolic living cabin, ensuring the stability of the gas entering the metabolic living cabin, reducing the rapid changes in the concentration of the detected gas caused by the rapid changes in the internal environmental gas of the metabolic living cabin, and flushing and correcting the sensor inside the concentration analyzer with standard gas, thereby reducing the accuracy of the detection data affected by the internal drift of the sensor inside the concentration analyzer.

[0029] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0031] Figure 1 It is an external axonometric diagram of the device of the present invention;

[0032] Figure 2It is a schematic diagram of the internal structure of the present invention;

[0033] Figure 3 It is a rear view schematic diagram of the internal structure of the present invention;

[0034] Figure 4 It is a system module diagram of the present invention;

[0035] Figure 5 is a flow chart of the method of the present invention;

[0036] Figure 6 This is the MR-t curve diagram of the propane combustion experiment of the present invention;

[0037] Figure 7 This is the RQ-t curve diagram of the propane combustion experiment of the present invention.

[0038] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0039] 1- metabolic chamber control group, 11- control room, 12- control room;

[0040] 2-Metabolic living cabin;

[0041] 3-equipment cabinet, 31-drying room, 32-EPC, 33-concentration analyzer, 34-control host, 35-intake fan, 36-exhaust fan, 37-standard gas cylinder group, 38-ruby gas resistance, 39-computer;

[0042] 4-Detection pipeline;

[0043] 5- Gas pipeline;

[0044] 6-buffer tank, 61-cabin, 62-diverter plate, 63-shutter;

[0045] 7-Exhaust solenoid valve;

[0046] 8-Intake solenoid valve. DETAILED DESCRIPTION

[0047] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] Accurate measurement of energy expenditure helps to study various issues related to how animals achieve or fail to achieve energy balance. One of the most accurate technologies that can be used to measure animal energy expenditure is the metabolic chamber using indirect calorimetry. The metabolic chamber is a device that uses indirect calorimetry to measure human metabolic expenditure. It measures the oxygen consumption and carbon dioxide production of the human body in the chamber, and uses the Weir formula to calculate the energy metabolic rate (MR, where MR is the speed at which animals convert food into energy, which is one of the important indicators of physical health) and respiratory entropy (RQ, where RQ can indicate the metabolic results of overnutrition or undernutrition, which is one of the important indicators of physical health). Multi-chamber animal metabolic chambers can be reused in multiple chambers, and multi-chamber metabolic chambers can measure metabolic data for multiple animals through cyclic measurement, making them suitable for use in animal husbandry. When using a metabolic chamber to detect metabolic data of animals, it is necessary to ensure the stability of the gas environment inside the chamber, because the slow and rapid changes in the gas inside the chamber will cause uneven distribution of CO2 and O2 gases in the gas, which will affect the results of subsequent sampling and detection. At the same time, the CO2 and O2 sensors of the gas analyzer will drift after working for a long time, and standard gases need to be introduced for calibration.

[0049] See also Figure 1-4 The present invention provides a technical solution: a multi-cabin multiplexed animal respiration heat measurement metabolic analysis system, comprising a metabolic cabin control group 1 and multiple metabolic living cabins 2, wherein the metabolic cabin control group 1 is composed of a control room 11 and an assembly wall 12, and the control room 11 is used for staff to enter to monitor metabolic data and command operation of detection equipment, the assembly wall 12 is used to place multiple metabolic living cabins 2, and the metabolic living cabins 2 and the assembly wall 12 are spliced ​​and combined by a dovetail mortise and tenon structure, and an equipment cabinet 3 for placing detection equipment and gas transmission equipment is arranged inside the control room 11, and the equipment cabinet 3 is a front and rear double-chamber design;

[0050] In the front room of the equipment cabinet 3, there are arranged from top to bottom a drying chamber 31 for detecting gas drying (the drying chamber 31 is a resistance wire heating drying tube, in which a temperature and humidity sensor is arranged), an EPC 32 for controlling the pressure input into the detection equipment (EPC 32 is an electronic pressure controller), a concentration analyzer 33 for analyzing and detecting the concentration of gas after drying (the concentration analyzer 33 is a URAS26 type infrared sensor and a differential pressure paramagnetic oxygen sensor analyzer, which can detect the concentration of CO2 and O2 gases, and the detection data acquisition frequency is 30 to 120 data points per minute), and a control host 34 for receiving detection data from the concentration analyzer 33, outputting detection data, and accepting and executing control commands (the control host 34 communicates with the concentration analyzer 33 via a 232 interface). In the rear room of the equipment cabinet 3, there are arranged an intake fan 35 for transporting external gas to the interior of the metabolic living cabin, an exhaust fan 36 for extracting gas from the interior of the metabolic living cabin 2, and a control host 34 for receiving detection data from the concentration analyzer 33, outputting detection data, and accepting and executing control commands. The control room 11 is provided with a standard gas cylinder group 37 for providing standard gas (the ratio of nitrogen, oxygen and carbon dioxide in the standard gas inside the standard gas cylinder group 37 is 79%, 21% and 0%), and the output end of the standard gas cylinder group 37 is connected with the output end of the exhaust fan 36 through a gas transmission solenoid valve, and the exhaust fan 36 and the standard gas cylinder group 37 can be switched by controlling the gas transmission solenoid valve to input the detection gas and the standard gas, and the drift phenomenon of the internal sensor of the concentration analyzer 33 caused by long-term detection can be corrected by flushing with standard gas. A desk for the staff is set inside the control room 11, and a computer 39 for receiving data from the control host 34 is placed on the desk (the computer 39 communicates with the control host 34 through a wireless network), and the computer 39 can also send control commands to the control host 34;

[0051] A detection pipeline 4 for collecting gas and a gas supply pipeline 5 for conveying gas are respectively arranged between the multi-group metabolic living cabin 2 and the drying chamber 31 and the air intake fan 35, and one end of the detection pipeline 4 passes through the partition between the front and rear chambers of the equipment cabinet 3 and is connected to the input end of the exhaust fan 36. A ruby ​​gas resistance 38 is arranged between the output end of the gas supply solenoid valve of the standard gas cylinder group 37 and the exhaust fan 36 and the input end of the drying chamber 31, and the other end of the ruby ​​gas resistance 38 passes through the partition between the front and rear chambers of the equipment cabinet 3 and is connected to the input end of the drying chamber 31 through a pipeline. The ruby ​​gas resistance 38 can control the flow rate of the extracted test gas entering the drying chamber 31 to 100 ml / min, avoiding the damage caused by the large gas flow rate. In order to prevent the impact damage of the electrical components inside the drying chamber 31, one end of the gas supply pipeline 5 is connected to the output end of the intake fan 35, an exhaust solenoid valve 7 is installed between adjacent detection pipelines 4, and an intake solenoid valve 8 is installed between two adjacent groups of gas supply pipelines 5, wherein the gas supply solenoid valve, multiple groups of exhaust solenoid valves 7 and multiple groups of intake solenoid valves 8 are all two-position three-way solenoid valves, and the multiple groups of exhaust solenoid valves 7 and the multiple groups of intake solenoid valves 8 are numbered and distinguished from left to right in the manner of one, two, three..., each group of exhaust solenoid valves 7 and intake solenoid valves 8 corresponds to a metabolic living cabin 2, and a buffer cabin 6 for transporting gas buffer is provided on the inner rear side of each group of metabolic living cabins 2, and the buffer cabin 6 is connected to the gas supply end of the gas supply pipeline 5;

[0052] The buffer cabin 6 includes a chamber 61 connected to the input end of the gas pipeline 5, and an arc-shaped diverter plate 62 is arranged in the inner cavity of the chamber 61, and the convex surface of the diverter plate 62 is arranged below the output end of the gas pipeline 5 in the inner cavity of the chamber 61. The arc-shaped design of the diverter plate 62 can buffer and divert the gas entering the chamber 61 through the output end of the gas pipeline 5, so that the gas can smoothly enter the metabolic living cabin 2, thereby reducing the input gas directly discharged into the interior of the metabolic living cabin 2 and causing the internal environmental gas to be unevenly distributed, thereby reducing the phenomenon of large fluctuations in the detected gas concentration caused by the rapid changes in the internal environmental gas of the metabolic living cabin 2. A shutter 63 connected to the inner cavity of the metabolic living cabin 2 is arranged on the front side of the chamber 61, and the shutter 63 can be used to uniformly transport the gas entering the cabin 61 to the inner cavity of the metabolic living cabin 2.

[0053] Please refer to 1-5, the present invention also provides a multi-cabin multiplexed animal respiration thermal metabolism analysis system detection method for detecting metabolic data, which has the following steps:

[0054] S1, empty chamber debugging: After the above-mentioned metabolic analysis system is built, empty chamber operation debugging is required to eliminate hardware problems such as pipeline leakage and abnormal operation of electrical equipment that affect the accuracy of detection data;

[0055] First, Figure 4 As shown ( Figure 4 The hollow arrow in the figure is the flow direction of the gas), and the computer 39 sends a command to the control host 34 to control the start of the air intake fan 35 and the exhaust fan 36, so that the external gas can be transported to the buffer cabin 6 through the air intake fan 35. After the diversion and buffering of the buffer cabin 6, the gas can be stably transported to the metabolic living cabin 2. Then, the computer 39 sends a command to the control host 34 to synchronously open the air intake solenoid valve 1 and the exhaust solenoid valve 1, and the air intake solenoid valves 8 and exhaust solenoid valves 7 of other groups are in a closed state, so that the gas can pass through the interior of the first group of metabolic living cabins 2, but cannot enter the interior of other metabolic living cabins 2. By applying soapy water at the connecting ends of each pipeline, it is judged whether there is a leakage phenomenon;

[0056] After eliminating the pipeline leakage, by observing the fluctuation of the data detected by the concentration analyzer 33 recorded in the computer 39, when the detected data no longer fluctuates greatly, it means that the electrical equipment can operate normally and stably. When the detected data always fluctuates greatly, it means that the electrical equipment has abnormal operation and needs to be eliminated. In this way, only the air intake solenoid valve 8 and the exhaust solenoid valve 7 on the same metabolic living cabin 2 are opened in turn each time, and the air intake solenoid valves 8 and the exhaust solenoid valves 7 on the other metabolic living cabins 2 are in a closed state, so that the pipeline leakage and the abnormal operation of the electrical equipment can be eliminated for each metabolic living cabin 2 in turn;

[0057] S2, data initialization: after eliminating the problems of pipeline leakage and abnormal operation of electrical equipment in step S1, in order to ensure the accuracy of the test data, it is necessary to flush and calibrate the concentration analyzer 33 with standard gas, so that the sensor inside the concentration analyzer 33 is in the initial calibration state, and the flushing and calibration time period t0 is recorded at the same time, so as to facilitate the setting of the flushing time again later, and to be able to flush and calibrate with standard gas again before the sensor inside the concentration analyzer 33 drifts;

[0058] In the step of eliminating the abnormal electrical operation of the equipment in step S1, the judgment is made by observing the fluctuation size of the detection data. On the basis of this step, the gas transmission solenoid valve is controlled by the computer 39 to connect the output end of the standard gas cylinder group 37 with the input end of the concentration analyzer 33, so that the standard gas in the standard gas cylinder group 37 can flush and calibrate the sensor inside the concentration analyzer 33. The concentration analyzer 33 is flushed and calibrated by the standard gas. The data drift between each detection data point and the standard gas (oxygen 21%) is obtained in the computer 39 using the differential function formula. When the drift is less than 1%, it means that the internal sensor of the concentration analyzer 33 is in the initial calibration state. Otherwise, it means that the internal sensor of the concentration analyzer 33 is in an unstable state. The concentration analyzer 33 needs to be continuously flushed with standard gas until its internal sensor is in the initial calibration state. At the same time, the time period t0 from flushing to calibration completion is recorded;

[0059] S3, metabolic data detection: after the internal sensor of the concentration analyzer 33 is in the initial calibration state in step S2, the experimental subject that needs to be detected for metabolic data is sent to the metabolic living cabin 2 for metabolic data detection. Before the experimental subject enters the metabolic living cabin 2, it is necessary to perform uniform cleaning and disinfection to reduce the impact of external microorganisms on the consumption of gas inside the metabolic living cabin 2;

[0060] The computer 39 then sets the air intake rate V of the air intake fan 35. 进气速率 is a fixed value, and the extraction gas rate V of the exhaust fan 36 is set 抽气速率 is a fixed value, and the gas velocity V entering the concentration analyzer 33 is limited by the ruby ​​gas resistance (38) 检测速率 is a fixed value, the drying temperature T of the drying chamber 31, the pressure data of EPC32 is Pa, and since the gas entering the metabolic living cabin 2 is the outside atmosphere, the gas concentration PCO of the entering CO2 can be known. 2进气 and O2 gas concentration PO 2进气 is a determined value;

[0061] At the same time, the cycle t of the concentration analyzer 33 collecting and detecting data is set through the computer 39, and the cycle of the standard gas flushing after the data collection cycle t is the time cycle t0 measured in step S2. The data collection of each metabolic living cabin 2 in each cycle t is independent, that is, when the metabolic living cabin 2 of this group collects data in the cycle t, the air inlet solenoid valve 8 and the exhaust solenoid valve 7 of other metabolic living cabins 2 are in a closed state, and only the gas in the metabolic living cabin 2 can be collected and detected. After the collected gas is dried in the drying chamber 31, it is released by the EPC32 and enters the concentration analyzer 33 to detect the CO2 concentration data and the O2 concentration data, and the PCO2 and PO2 data are obtained. The detected PCO2 and PO2 data are transmitted to the computer 39 through the control host 34;

[0062] S4, calculation of metabolic data: Based on the CO2 concentration data PCO2 and O2 concentration data PCO2 detected in step S3, the CO2 change rate VCO2 and O2 change rate VO2 of the experimental subject in the metabolic living cabin 2 are calculated in the computer 39, and the calculation formula is as follows:

[0063] VCO2=(PCO2-PCO 2进气 )×(V 进气速率 -V 检测速率 )

[0064] VO2=(PO 2进气 -PO2)×(V 进气速率 -V 检测速率 )

[0065] According to the calculated CO2 change rate VCO2 and O2 change rate VO2 data, the metabolic rate MR and respiratory entropy RQ of the experimental subject in the metabolic living chamber 2 are further calculated, and the metabolic rate MR of the experimental subject is calculated by the Weir equation, as follows

[0066] MR=(k1×VCO2)+(k2×VO2)

[0067] Among them, k1 is the metabolic coefficient of the experimental subject's respiratory metabolism to produce CO2, and k2 is the metabolic coefficient of the experimental subject's respiratory metabolism to consume O2;

[0068] The calculation formula of respiratory entropy RQ is:

[0069]

[0070] Based on the calculated metabolic rate MR data and respiratory entropy RQ data, combined with the period t during the detection process, the MR-t curve and RQ-t curve are drawn using the Smooth function in the computer 39. The staff judges the metabolic stability of the experimental subject by observing the fluctuations of the MR-t curve and the RQ-t curve.

[0071] Please refer to 1-7. This scheme uses a propane combustion experiment based on the above-mentioned metabolic chamber and method to verify whether the above-mentioned metabolic chamber can reduce the changes in ambient gases that affect the fluctuations in the concentration of the detected gas, thereby ensuring the accuracy of the detected data.

[0072] The propane combustion experiment is set up with two groups of metabolic chambers. The experimental group is built according to the above-mentioned metabolic chamber, and the control group is a method in which the buffer air chamber 6 is dismantled so that the input end of the gas pipe 5 is directly connected to the inner cavity of the metabolic living chamber 2. At the same time, the standard gas is not used to flush and calibrate the sensor inside the concentration analyzer 33. The other structures are the same as the experimental group. After eliminating the problems of gas leakage in the metabolic chamber pipeline and abnormal operation of electrical equipment and initializing the gas environment inside the two metabolic chambers, the test parameters are set by the computer 39. The specific settings are shown in the following table:

[0073]

[0074]

[0075] After setting up the above table, the metabolic rate MR data and respiratory entropy RQ data and MR-t curve and RQ-curve of the experimental group and the control group can be obtained through the above method. At the same time, according to the propane combustion formula

[0076] C3H8+5O2→3CO2+4H2O

[0077] The theoretical metabolic rate MR data and respiratory entropy RQ data and MR-t curve and RQ-t curve in the propane combustion process can be calculated, and the experimental group, the control group and the theoretically calculated MR-t curve are placed in the same coordinate system, and the experimental group, the control group and the theoretically calculated RQ-t curve are placed in the same coordinate system for comparative analysis, such as Figure 6 and Figure 7 As shown (due to the large number of detected data points, the generated curve is relatively dense, Figure 6 and Figure 7 The curve in the figure is a section of the whole curve that is intercepted and amplified to facilitate the observation and judgment of the later staff). Through observation, it can be seen that the fluctuations of the two groups of curves in the experimental group are smaller than those in the control group, which shows that the metabolic chamber of this scheme has a certain anti-interference ability;

[0078] The computer 39 then calculates the percentage average SD between the experimental group and the theoretically calculated metabolic rate MR data and respiratory entropy RQ data, and between the control group and the theoretically calculated metabolic rate MR data and respiratory entropy RQ data, as shown in the following table:

[0079]

[0080]

[0081] According to the percentage average SD value between the metabolic rate MR data and the respiratory entropy RQ data in the above table, it can be known that the data of the experimental group are higher than those of the control group, which can further illustrate that the use of the buffer chamber in this scheme can ensure the stability of the gas entering the metabolic living chamber, reduce the rapid changes in the detection gas concentration caused by the rapid changes in the environmental gas inside the metabolic living chamber, and through the flushing and correction of the sensor inside the concentration analyzer with standard gas, reduce the accuracy of the detection data affected by the internal drift of the sensor inside the concentration analyzer.

[0082] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0083] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A multi-cabin multiplexed animal respiration thermometry metabolic analysis system, comprising a metabolic chamber control group (1) and multiple metabolic living chambers (2), characterized in that: A plurality of groups of metabolic living cabins (2) are installed on one side of a metabolic cabin control group (1); an equipment cabinet (3) for accommodating detection equipment and gas transmission equipment is arranged inside the metabolic cabin control group (1); a detection pipeline (4) for collecting gas and a gas transmission pipeline (5) for transmitting gas are arranged between the equipment cabinet (3) and the plurality of groups of metabolic living cabins (2), respectively; a buffer cabin (6) for transmitting gas buffer is arranged on the rear side inside each group of metabolic living cabins (2); the buffer cabin (6) is connected to the gas transmission end of the gas transmission pipeline (5); an exhaust solenoid valve (7) is installed between two adjacent groups of detection pipelines (4); and an intake solenoid valve (8) is installed between two adjacent groups of gas transmission pipelines (5); The buffer cabin (6) comprises a chamber (61) connected to the input end of the gas pipeline (5); the inner cavity of the chamber (61) is provided with a diverter plate (62) of arc design, and the convex surface of the diverter plate (62) is arranged below the output end of the gas pipeline (5) in the inner cavity of the chamber (61); and the front side surface of the chamber (61) is provided with a shutter (63) connected to the inner cavity of the metabolic living chamber (2).

2. A multi-chamber multiplexed animal respiration thermal metabolism analysis system according to claim 1, characterized in that: The metabolic cabin control group (1) comprises a control room (11) and an assembly wall (12); the assembly wall (12) and the metabolic living cabin (2) are spliced ​​and assembled via a mortise and tenon structure; and the equipment cabinet (3) is arranged inside the control room (11).

3. A multi-chamber multiplexed animal respiration thermal metabolism analysis system according to claim 2, characterized in that: The equipment cabinet (3) is designed with a front and rear double chamber. The front chamber of the equipment cabinet (3) is provided with a drying chamber (31), an EPC (32), a concentration analyzer (33) and a control host (34) in order from top to bottom. The rear chamber of the equipment cabinet (3) is provided with an air intake fan (35), an exhaust fan (36), a standard gas cylinder group (37) and a ruby ​​air block (38). The control chamber (11) is provided with a computer (39). The drying chamber (31) is used to detect the drying of the gas, the EPC (32) is used to control the pressure input into the detection device, the concentration analyzer (33) is used to analyze and detect the concentration of the gas after drying, the control host (34) is used to receive the detection data of the concentration analyzer (33), output the detection data, receive and execute control commands, the air intake fan (35) is used to transport the external gas into the metabolic living cabin (2), the exhaust fan (36) is used to extract the gas inside the metabolic living cabin (2) for detection, the standard gas cylinder group (37) is used to provide standard gas for flushing and calibration, the ruby ​​gas resistance (38) is used to limit the flow rate of the outflowing detection gas, and the computer (39) is used to receive data from the control host (34) and send control commands to the control host (34).

4. A multi-chamber multiplexed animal respiration thermal metabolism analysis system according to claim 3, characterized in that: The output end of the standard gas cylinder group (37) is connected to the output end of the exhaust fan (36) through a gas supply solenoid valve, the input end of the exhaust fan (36) is connected to one end of the detection pipeline (4), and the detection pipeline (4) passes through the partition between the front and rear chambers of the equipment cabinet (3), the ruby ​​gas resistor (38) is arranged between the output end of the gas supply solenoid valve and the input end of the drying chamber (31), and the other end of the ruby ​​gas resistor (38) is connected to the input end of the drying chamber (31) through a pipeline passing through the partition between the front and rear chambers of the equipment cabinet (3).

5. The multi-chamber multiplexed animal respiration thermal metabolism analysis system according to claim 4, characterized in that: The drying chamber (31) is a resistance wire heating drying tube, the EPC (32) is an electronic pressure controller, the control host (34) and the concentration analyzer (33) communicate via a 232 interface, and the computer (39) and the control host (34) communicate via a wireless network.

6. The multi-chamber multiplexed animal respiration thermal metabolism analysis system according to claim 5, characterized in that: The proportions of nitrogen, oxygen and carbon dioxide in the standard gas inside the standard gas cylinder group (37) are 79%, 21% and 0% respectively.

7. A detection method for a multi-chamber multiplexed animal respiration thermal measurement metabolic analysis system, used to implement the metabolic analysis system described in claims 1-6, the specific steps are as follows: S1, empty cabin debugging, to eliminate pipeline leakage and abnormal operation of electrical equipment; S2, data initialization, so that its internal sensors are in the initial calibration state; S3, metabolic data detection: after the internal sensor of the concentration analyzer (33) is in an initial calibration state in step S2, the experimental subject that needs to undergo metabolic data detection is sent into the metabolic living cabin (2) for metabolic data detection. Before the experimental subject enters the metabolic living cabin (2), it is necessary to perform uniform cleaning and disinfection to reduce the impact of external microorganisms on the consumption of gas inside the metabolic living cabin (2); The air intake rate V of the air intake fan (35) is then set by the computer (39). 进气速率 is a fixed value, and the extraction gas rate V of the exhaust fan (36) is set 抽气速率 is a fixed value, and the gas velocity V entering the concentration analyzer (33) is limited by the ruby ​​gas resistance (38) 检测速率 is a fixed value, the drying temperature of the drying chamber (31) is T, the pressure data of the EPC (32) is Pa, and since the gas entering the metabolic living cabin (2) is the outside atmosphere, the gas concentration of the CO2 entering is PCO 2进气 and O2 gas concentration PO 2进气 is a determined value; At the same time, the cycle t of the concentration analyzer (33) collecting and detecting data is set by the computer (39), and the cycle of the standard gas flushing after the data collection cycle t is the time cycle t0 measured in step S2. The data collection of each metabolic living cabin (2) in each cycle t is independent, that is, when the metabolic living cabin (2) of this group is collecting data in the cycle t, the air inlet solenoid valve (8) and the exhaust solenoid valve (7) of other metabolic living cabins (2) are in a closed state, and only the gas in the metabolic living cabin (2) can be collected and detected. After the collected gas is dried in the drying chamber (31), it is released by the EPC (32) and then enters the concentration analyzer (33) to detect the CO2 concentration data and the O2 concentration data, and obtains the PCO2 and PO2 data. The detected PCO2 and PO2 data are transmitted to the computer (39) through the control host (34); S4, calculation of metabolic data: Based on the CO2 concentration data PCO2 and O2 concentration data PCO2 detected in step S3, the CO2 change rate VCO2 and O2 change rate VO2 of the experimental subject in the metabolic living chamber (2) are calculated in the computer (39), and the calculation formula is as follows: VCO2=(PCO2-PCO 2进气 )×(V 进气速率 -V 检测速率 ) VO2=(PO 2进气 -PO2)×(V 进气速率 -V 检测速率 ) According to the calculated CO2 change rate VCO2 and O2 change rate VO2 data, the metabolic rate MR and respiratory entropy RQ of the experimental subject in the metabolic living chamber (2) are further calculated, and the metabolic rate MR of the experimental subject is calculated by the Weir equation, as follows: MR=(k1×VCO2)+(k2×VO2) in, k1 is the metabolic coefficient of the experimental subject's respiratory metabolism to produce CO2, and k2 is the metabolic coefficient of the experimental subject's respiratory metabolism to consume O2; The calculation formula of respiratory entropy RQ is: Based on the calculated metabolic rate MR data and respiratory entropy RQ data, combined with the period t during the detection process, the MR-t curve and RQ-t curve are drawn using the Smooth function in a computer (39).

8. A detection method for a multi-chamber multiplexed animal respiration, thermal and metabolic analysis system according to claim 7, characterized in that: S1, the specific steps of empty cabin commissioning include: A command for starting the air intake fan (35) and the air exhaust fan (36) is sent to the control host (34) through the computer (39), so that the external gas can be transported to the buffer cabin (6) through the air intake fan (35). After the diversion and buffering of the buffer cabin (6), the gas can be stably transported to the metabolic living cabin (2). Then, a command is sent to the control host (34) through the computer (39) to synchronously open the air intake solenoid valve 1 and the exhaust solenoid valve 1. The air intake solenoid valves (8) and the exhaust solenoid valves (7) of other groups are all in a closed state, so that the gas can pass through the interior of the first group of metabolic living cabins (2) but cannot enter the interior of other metabolic living cabins (2). By applying soapy water to the connection ends of each pipeline, it is judged whether there is a leakage phenomenon; After eliminating the pipeline leakage, the fluctuation of the data detected by the concentration analyzer (33) recorded in the computer (39) is observed. When the detected data no longer shows large fluctuations, it means that the electrical equipment can operate normally and stably. When the detected data continues to show large fluctuations, it means that the electrical equipment has an abnormal operation and needs to be eliminated. Each time, only the air intake solenoid valve (8) and the exhaust solenoid valve (7) on the same metabolic living cabin (2) are opened, and the air intake solenoid valves (8) and the exhaust solenoid valves (7) on the other metabolic living cabins (2) are in a closed state. The pipeline leakage and the abnormal operation of the electrical equipment can be eliminated for each metabolic living cabin (2) in turn.

9. A detection method for a multi-chamber multiplexed animal respiration, thermal and metabolic analysis system according to claim 7, characterized in that: S2, data initialization specifically includes: In the step of eliminating electrical abnormalities of the equipment in step S1, the judgment is made by observing the fluctuation size of the detection data. Based on this step, the gas supply solenoid valve is controlled by the computer (39) to connect the output end of the standard gas cylinder group (37) with the input end of the concentration analyzer (33), so that the standard gas in the standard gas cylinder group (37) can flush and calibrate the sensor inside the concentration analyzer (33). The concentration analyzer (33) is flushed with the standard gas, and the data drift between each detection data point and the standard gas is obtained in the computer (39) using a differential function formula. When the drift is less than 1%, it means that the internal sensor of the concentration analyzer (33) is in an initial calibration state. Otherwise, it means that the internal sensor of the concentration analyzer (33) is in an unstable state, and the concentration analyzer (33) needs to be continuously flushed with standard gas until its internal sensor is in an initial calibration state. At the same time, the time period t0 from flushing to calibration completion is recorded.

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