A method for priming corn seeds using nanosilver particles

Abstract

The invention relates to the field of agricultural biotechnology, and in particular to a method for priming corn seeds by using nano-silver particles. The method comprises the following steps: selecting healthy corn seeds free of pests and diseases, and soaking and disinfecting the corn seeds in a sodium hypochlorite solution with a mass concentration of 0.5%-1%, soaking the corn seeds, and maintaining the soaking temperature of the corn seeds in the treatment solution at 25-30°C and the ambient humidity at 60%-70%, shaking the soaked corn seeds, taking out the corn seeds after soaking for a period of time, washing them with deionized water, removing excess treatment solution, placing the corn seeds in a ventilated and cool place to dry, and germinating the dried corn seeds. The invention prepares the nano-silver particle solution by using a citric acid reduction method, is simple to operate, and has low cost. The nano-silver particles can significantly improve the germination rate and pest resistance of the corn seeds when treating the corn seeds, and the treated seeds can effectively enhance the stress resistance and growth vitality of the seeds, and have high promotion and application value.

Description

Technical Field

[0001] The invention relates to the field of agricultural biotechnology, and in particular to a method for priming corn seeds using nano silver particles. Background Art

[0002] With the requirements of agricultural modernization and the frequent natural disasters caused by changes in corn planting climate conditions, corn seeds need to ensure better germination ability in adverse environments. Traditional crop seed treatment methods can no longer meet the requirements of modern agriculture for seed quality, germination speed, resistance to diseases and insect pests, etc. In recent years, the application of nanotechnology in the field of agriculture has achieved remarkable results, especially in the treatment of crop seeds. Nanosilver particles can effectively improve the germination performance of seeds, promote growth and improve the ability of crops to resist diseases and insect pests. However, the prior art lacks a method for preparing nanomaterials that can simultaneously have both high efficiency and environmental protection, and there are still many deficiencies in the treatment method for corn seeds. Summary of the invention

[0003] The purpose of the present invention is to provide a method for priming corn seeds using nano-silver particles, so as to solve the problems of unsatisfactory priming treatment effect of corn seeds, complex priming process and high cost.

[0004] The present invention provides the following technical solution: a method for priming corn seeds using nanosilver particles, comprising step S100, selecting healthy corn seeds without pests and diseases, soaking and disinfecting them in a sodium hypochlorite solution with a mass concentration of 0.5%-1%, then rinsing them with clean water for 3-5 times, draining the water and setting aside; Step S200, soaking the corn seeds treated in step S100, wherein the mass ratio of the treatment liquid to the corn seeds is 1:5; Keep the temperature of corn seeds soaked in the treatment solution at 25-30°C and the ambient humidity at 60%-70%; Step S100, performing an oscillation treatment on the soaked corn seeds so that the treatment liquid is in uniform contact with the surface of the corn seeds; Step S400, after soaking for a period of time, taking out the corn seeds and rinsing them with deionized water, removing the excess treatment liquid and placing them in a ventilated and cool place to dry until there is no excess liquid on the surface of the corn seeds; Step S500, performing germination treatment on the dried corn seeds, placing the corn seeds in a constant temperature incubator at a temperature of 25-28° C. and a humidity of 80%-90%, using moist sterile gauze or culture medium; Step S600, observing and recording various indicators of the corn seeds treated in step S500 and analyzing the germination results.

[0005] Preferably, the treatment liquid is a colloidal solution of nanosilver particles, and the preparation method thereof comprises: Dissolve silver nitrate in deionized water to prepare a 1 mM silver nitrate solution; Take sodium citrate dihydrate, add deionized water and stir to completely dissolve it to prepare a 0.5 mM sodium citrate solution; After the silver nitrate solution is heated to boiling under a reverse condenser, a sodium citrate solution is added under strong magnetic stirring, the molar ratio of silver nitrate to sodium citrate is 1:3-4, a brown dispersion is formed within 15 minutes after boiling, and the obtained suspension is stirred again under reflux to complete the reaction, and a green-gray Ag particle colloid is obtained; The unreacted impurities are removed by filtration, and the product is washed 1-3 times with deionized water to remove excess citric acid residue to obtain a nanosilver particle colloidal solution.

[0006] Compared with the prior art, the present invention has the following beneficial effects: The invention uses a citric acid reduction method to prepare a nano silver particle solution, which is simple to operate and low in cost, and the nano silver particles can significantly improve the germination rate and pest resistance of corn seeds when treating them. The corn seeds treated by the method of the invention can effectively enhance the stress resistance and growth vitality of the seeds, and have a high value for promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 is a flow chart of the method of the present invention; Figure 2 is a flow chart for preparing the treatment solution in the method of the present invention; Figure 3 This is a comparison chart of the experiment of the present invention. DETAILED DESCRIPTION

[0008] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0009] In order to solve the problems of unsatisfactory priming treatment effect of corn seeds, complicated priming process and high cost in the prior art, the present invention provides a method for priming corn seeds using nanosilver particles. The method prepares nanosilver particle solution by citric acid reduction method, which is simple, efficient and low-cost, and can significantly improve the germination rate and resistance of corn seeds.

[0010] refer to Figure 1The present invention provides the following technical scheme: a method for priming corn seeds using nanosilver particles, comprising step S100, selecting healthy corn seeds without pests and diseases, soaking and disinfecting them in a sodium hypochlorite solution with a mass concentration of 0.5%-1% for 5 minutes, then rinsing them with clean water for 3-5 times, and draining the water; step S200, soaking the corn seeds treated in step S100 in a treatment solution for 20 hours (the time can be selected and adjusted according to experimental conditions and characteristics of corn varieties), wherein the mass ratio of the treatment solution to the corn seeds is 1:5; maintaining the soaking temperature of the corn seeds in the treatment solution at 25-30°C and the ambient humidity at 60%-70%; step S100, shaking the soaked corn seeds (shaking the container gently every 10-15 minutes) or placing them on a shaking table (40rpm / h) to make the corn seeds The treatment liquid is in uniform contact with the surface of the corn seeds; step S400, after soaking for a period of time, the corn seeds are taken out and rinsed with deionized water, and the excess treatment liquid is removed and placed in a ventilated and cool place to dry until there is no excess liquid on the surface of the corn seeds; step S500, the dried corn seeds are subjected to germination treatment, and the corn seeds are placed in a constant temperature incubator at a temperature of 25-28°C and a humidity of 80%-90%, using a moist sterile gauze or culture medium; step S600, the various indicators of the corn seeds treated in step S500 are observed, recorded, and the germination results are analyzed (through comparative experiments, the differences in germination rate, pest resistance, seedling growth, etc. between the corn seeds treated by the present invention and the untreated seeds are evaluated, and according to the experimental results, the concentration of the nanosilver particle solution, the soaking time and other treatment conditions are adjusted to optimize the treatment effect). Observe the germination rate, germination speed, seedling growth status and other indicators of the seeds, and record the changes in the germination rate and germination speed.

[0011] refer to Figure 2 In the above scheme, the treatment liquid is a colloidal solution of nanosilver particles, and its preparation method includes: Silver nitrate is dissolved in deionized water to prepare a 1 mM silver nitrate solution; sodium citrate dihydrate is added to deionized water and stirred to completely dissolve it to prepare a 0.5 mM sodium citrate solution; the silver nitrate solution is heated to boiling (80-100°C) under a reverse condenser, and then sodium citrate solution is added under strong magnetic stirring, the molar ratio of silver nitrate to sodium citrate is 1:3-4, a brown dispersion is formed within 15 minutes after boiling, and the obtained suspension is stirred under reflux for another 30 minutes to complete the reaction, and a green-gray Ag particle colloid is obtained (in actual experimental results, it remains stable after being stored at room temperature for at least several months. AgNPs may precipitate, but can be easily re-dispersed by mechanical shaking); unreacted impurities are filtered to remove, and the product is washed with deionized water 1-3 times to remove excess citric acid residues to obtain a nanosilver particle colloid solution. Example

[0012] 0.05 M silver nitrate solution and 0.2 M citric acid solution were mixed in a volume ratio of 1:1 and heated at 50°C for 2 hours to obtain a colloidal solution of nanosilver particles. 100 corn seeds were selected, soaked in the solution for 60 minutes, and then dried for germination experiments. The results showed that the germination rate of corn seeds after treatment increased by 15% compared with the control, and after water shortage treatment, they showed better drought resistance. Example

[0013] 0.1 M silver nitrate solution and 0.3 M citric acid solution were mixed in a volume ratio of 1:1 and heated at 55°C for 1 hour to obtain a colloidal solution of nanosilver particles. 100 corn seeds were selected, soaked in the solution for 90 minutes, and then dried for germination experiments. The results showed that the germination rate of corn seeds after treatment increased by 18% compared with the control, and the germination speed of seeds was accelerated under drought conditions, and the growth performance was healthier. Example

[0014] 0.08 M silver nitrate solution and 0.25 M citric acid solution were mixed in a volume ratio of 1:1 and heated at 52°C for 1.5 hours to obtain a nanosilver solution. 100 corn seeds were selected, soaked in the solution for 45 minutes, and then dried for germination experiments. The results showed that the germination rate of corn seeds after treatment increased by 12% compared with the control, and the growth rate of seedlings in adverse environments was significantly accelerated, showing better drought resistance.

[0015] refer to Figure 3 After testing the germination ability of commercially available corn seeds, it was found that the germination ability of seeds was significantly enhanced after being triggered by nanosilver particles in a laboratory environment ( Figure 3 (Comparison of germination test after treatment for 60 hours and normal germination test without treatment for 60 hours). At the same time, when the water shortage treatment was applied at 60 hours, the seeds primed by nanosilver particles were significantly superior to the control.

[0016] The above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them.

Claims

1. A method for priming corn seeds using nanosilver particles, characterized in that: include: Step S100, selecting healthy corn seeds without diseases and insect pests, soaking and disinfecting them in a sodium hypochlorite solution with a mass concentration of 0.5%-1%, then rinsing them with clean water for 3-5 times, and draining the water for later use; Step S200, soaking the corn seeds treated in step S100, wherein the mass ratio of the treatment liquid to the corn seeds is 1:5; Keep the temperature of corn seeds soaked in the treatment solution at 25-30°C and the ambient humidity at 60%-70%; Step S100, performing an oscillation treatment on the soaked corn seeds so that the treatment liquid is in uniform contact with the surface of the corn seeds; Step S400, after soaking for a period of time, taking out the corn seeds and rinsing them with deionized water, removing the excess treatment liquid and placing them in a ventilated and cool place to dry until there is no excess liquid on the surface of the corn seeds; Step S500, performing germination treatment on the dried corn seeds, placing the corn seeds in a constant temperature incubator at a temperature of 25-28° C. and a humidity of 80%-90%, using moist sterile gauze or culture medium; Step S600, observing and recording various indicators of the corn seeds treated in step S500 and analyzing the germination results; The treatment liquid is a colloidal solution of nano silver particles, and its preparation method comprises: Dissolve silver nitrate in deionized water to prepare a 1 mM silver nitrate solution; Take sodium citrate dihydrate, add deionized water and stir to completely dissolve it to prepare a 0.5 mM sodium citrate solution; After the silver nitrate solution is heated to boiling under a reverse condenser, a sodium citrate solution is added under strong magnetic stirring, the molar ratio of silver nitrate to sodium citrate is 1:3-4, a brown dispersion is formed within 15 minutes after boiling, and the obtained suspension is stirred again under reflux to complete the reaction, and a green-gray Ag particle colloid is obtained; wherein the heating temperature is 50°C-55°C; and the heating is performed for 1-2 hours; The unreacted impurities are removed by filtration, and the product is washed 1-3 times with deionized water to remove excess citric acid residue to obtain a nanosilver particle colloidal solution.

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