Method for prolonging spawning period of epinephelus moara

Abstract

The invention discloses a method for prolonging the spawning period of epinephelus moara, which comprises the following steps: (1) selecting juvenile epinephelus moara with the body weight of 100-150g as a parent fish rearing object, with the dissolved oxygen of 5-9mg / L, the salinity of 25-33, the pH of 7.8-8.2, the water flow exchange capacity of 2-3m < 3 > / h, the water temperature of 25-28 DEG C, the sunlight illumination time of 13.5 h and the rearing density of 6-8kg / m < 3 >; the feed is fed once a day, and the traditional Chinese medicine composition is taken for 10d / month; (2) when the parent fish reaches the sexual maturity age, the water temperature is reduced to 20 DEG C, the sunlight illumination time is 12 h, and the breeding density is 6-8 kg / m < 3 >; the feed is fed twice and three times every day, and the traditional Chinese medicine composition is taken for 10d / month; after one month, the water temperature is gradually increased to 25 DEG C, and the daily illumination time is gradually increased to 13.5 h; (3) when the gonad of the parent fish develops to 3-4 stages, injecting chorionic gonad promoting hormone for spawning induction; (4) after the parent fishes lay eggs for 30 days, the water temperature is gradually reduced to 20 DEG C, and meanwhile, the illumination time is gradually reduced to 12 hours; after 30 days, the water temperature is gradually increased to 25 DEG C, and the illumination time is gradually increased to 13.5 h. According to the method, the breeding period of the parent fish of the epinephelus moara is prolonged.

Description

Technical Field

[0001] The invention relates to a method for prolonging the spawning period of Epinephelus moire, and belongs to the technical field of breeding Epinephelus moire. Background Art

[0002] The clouded grouper (commonly known as the real oily grouper) has delicious meat and high economic value. Because of its delicious meat, high economic value and large market demand, it is the main species of grouper exported to Japan and South Korea. The "Yunlong grouper" hybridized with the clouded grouper as the female parent and the saddle-banded grouper male has the characteristics of good meat quality, fast growth rate and strong cold resistance. Since it was approved as a new aquatic species by the state, the breeding scale has been continuously expanded. It has now become the first choice for new grouper breeding in Fujian and Guangdong. However, due to the short spawning period of the parent fish of the clouded grouper under natural conditions (generally from March to April each year in the coastal areas of Fujian and Guangdong, about 30 days), the parent fish lay fewer eggs, which affected the production of fertilized eggs of the hybrid "Yunlong grouper", resulting in a slow growth in the scale of "Yunlong grouper" seedling production, which still cannot meet the needs of breeding production, and the development of the industry is greatly restricted. Summary of the invention

[0003] The present invention provides a method for extending the spawning period of Epinephelus moire, which can effectively solve the above problems. The present invention is implemented as follows:

[0004] A method for extending the spawning period of Epinephelus mole, comprising the following steps:

[0005] (1) Young Epinephelus moire with a body length of 20 to 25 cm and a body weight of 100 to 150 g were selected as broodstock for cultivation in a factory-based aquaculture environment. The cultivation conditions were controlled as follows: dissolved oxygen 5 to 9 mg / L, salinity 25 to 33, pH 7.8 to 8.2, and water exchange volume 2 to 3 m 3 / h, water temperature 25-28℃, daylight duration 13.5h, cultivation density controlled at 6-8kg / m 3 ; Feed 1 once a day, and the feeding amount is based on the fact that the broodstock will no longer compete for food. At the same time, the Chinese medicine composition is added to Feed 1 and fed together for 10 days every month; The water temperature for cultivation from seedlings to adult fish is controlled in the optimal growth temperature range of 25-28℃, and constant temperature breeding is adopted. The entire growth period does not experience low temperature and high temperature periods (the lowest temperature in Fujian Province in winter can reach about 10℃ and the highest temperature can reach about 31℃), which increases the growth rate and shortens the maturity period from the original 4 years to 3 years.

[0006] (2) When the broodstock reach sexual maturity (over 36 months), starting from December each year, 1 to 2 months before spawning, the water temperature is lowered to 20°C, the daylight duration is controlled to 12 hours, and the breeding density is controlled to 6 to 8 kg / m 3; Feed 2 and 3 once a day until the broodstock no longer compete for food. At the same time, add the Chinese medicine composition to feed 2 and 3 for 10 days every month and feed them together; After 1 month, gradually increase the cultivation water temperature to 25℃ and the daylight duration to 13.5h; Promote the development of broodstock gonads through coordinated regulation of light cycle and water temperature.

[0007] (3) When the gonads of the broodstock develop to stage 3-4, chorionic gonadotropin or luteinizing hormone-releasing hormone analogs are injected to induce spawning;

[0008] (4) 30 days after the broodstock spawn, the water temperature is gradually reduced to 20°C, and the light duration is gradually reduced to 12 hours; 30 days later, the water temperature is gradually increased to 25°C, and the light duration is gradually increased to 13.5 hours, so that the gonads of the broodstock develop and mature again, and the second spawning can be achieved; this process is repeated, and a batch of broodstock can spawn once in 2 months;

[0009] Feed 1: 30-40 parts of fish meal, 10-20 parts of corn flour, 5-10 parts of soybean meal, 5-10 parts of rice bran, 3-5 parts of fish oil, 3-5 parts of enteromorpha, 10-15 parts of α-starch, 1.5-2.5 parts of soybean lecithin, 10-20 parts of artemia powder, 0.3-0.5 parts of chitosan, 10-15 parts of Antarctic krill powder, 1 part of vitamin mixture, 1 part of mineral mixture, 0.03-0.04 parts of compound microecological preparation. The feed is in soft granular form with a particle size of 1-3 mm.

[0010] Feed 2: 40-50 parts of fish meal, 10-20 parts of corn flour, 5-10 parts of soybean meal, 5-10 parts of rice bran, 3-5 parts of fish oil, 3-5 parts of enteromorpha, 10-15 parts of α-starch, 1.5-2.5 parts of soybean lecithin, 5-10 parts of artemia powder, 0.3-0.5 parts of chitosan, 10-15 parts of Antarctic krill powder, 1 part of vitamin mixture, 1 part of mineral mixture, 0.03-0.04 parts of compound microecological preparation, 0.04-0.05 parts of VE. The feed is made into soft granules with a particle size of 10-20 mm.

[0011] Feed 3: 1 portion of fresh shrimp meat, 1 portion of fresh squid;

[0012] The Chinese medicine composition comprises: 15-20 parts of astragalus, 10-15 parts of honeysuckle, 5-10 parts of phellodendron, 10-15 parts of eucommia leaf powder, 10-15 parts of liquorice, 10-15 parts of hawthorn, 5-10 parts of atractylodes, 5-10 parts of safflower, 10-15 parts of acanthopanax, 5-10 parts of magnolia bark, 10-15 parts of plantain, and 5-10 parts of senna leaves;

[0013] The composite microecological preparation is composed of lactobacillus, bacillus subtilis, and yeast in a mass ratio of 1:1:1, and the content of the mixed bacteria is 1×107 cfu / g~1×10 9 cfu / g.

[0014] The mixing ratio of the three different beneficial microorganisms in the composite microecological preparation ensures that each microorganism can play its unique role in the preparation, thereby achieving the best synergistic effect. Its content range ensures the activity and quantity of the microorganisms in the preparation, so that it can play the maximum effect in practical applications.

[0015] This composite microecological preparation has a significant effect in improving the hatching rate of long-moire grouper fry. By using this preparation, the hatching environment can be effectively improved, the survival ability of fry can be enhanced, and the hatching rate can be improved. This has important practical application value for the aquaculture industry and helps to improve breeding efficiency and economic benefits.

[0016] The feeds 1-3 are configured according to the specific nutritional needs of fish at different growth stages. These feeds are rich in protein, vitamins and minerals, and can meet the special needs of fish at various growth stages. Through scientific formulas, these feeds are not only conducive to the healthy growth of fish, but also effectively promote the development of their gonads, thereby further improving the spawning rate. This targeted nutrition supply method ensures that fish can obtain the best nutritional support at each growth stage, thereby achieving the best growth effect and reproductive effect.

[0017] In particular, fresh shrimp meat and fresh squid are added to feed 3 to provide rich nutrition for sexual maturity and development, and promote gonadal development and ovulation.

[0018] Smaller particle sizes are more conducive to the feeding of young fish. This is because the mouth and digestive tract of young fish are relatively small, and smaller feed particles can be caught and swallowed more easily by them. In addition, small feed particles are more evenly dispersed in the water, which helps young fish to absorb nutrients more effectively, thus promoting their healthy growth. Therefore, when raising young fish, it is very important to choose feed with smaller particle sizes.

[0019] In addition, making the feed into soft granules is also beneficial to the eating, digestion and absorption of young fish, thereby promoting the healthy growth and sexual maturity of fish.

[0020] In some specific embodiments, in order to effectively promote the spawning process of fish, a method of injecting chorionic gonadotropin is adopted. Specifically, the injection dose is usually set in the range of 500 to 1000 micrograms per kilogram of fish body. In this way, the reproductive system of fish can be effectively stimulated, thereby achieving the effect of inducing spawning, and then promoting the spawning behavior of fish.

[0021] In other specific embodiments, in order to further optimize the spawning process of fish, luteinizing hormone releasing hormone analogs are used for injection. In this case, the injection dose is generally 2 to 5 micrograms per kilogram of fish. By accurately controlling this dose, the natural physiological process can be effectively simulated, thereby achieving the purpose of inducing spawning and further promoting the spawning behavior of fish.

[0022] In certain specific embodiments, the amount of the Chinese medicine composition added to the animal feed is generally 0.1 to 0.2 weight percent (wt%). This means that when preparing animal feed, the Chinese medicine composition needs to be added at a ratio of 0.1% to 0.2% of the total weight of the feed to ensure that the animal can ingest an appropriate amount of Chinese medicine ingredients to achieve the desired health-promoting effect. By precisely controlling the amount of addition, it can be ensured that the concentration of the Chinese medicine composition in the feed is neither too high to cause potential side effects nor too low to lose its due efficacy.

[0023] A Chinese medicine composition for prolonging the spawning period of long-moire grouper, comprising: 15 to 20 parts of astragalus, 10 to 15 parts of honeysuckle, 5 to 10 parts of phellodendron, 10 to 15 parts of eucommia leaf powder, 10 to 15 parts of liquorice, 10 to 15 parts of hawthorn, 5 to 10 parts of atractylodes, 5 to 10 parts of safflower, 10 to 15 parts of acanthopanax, 5 to 10 parts of magnolia bark, 10 to 15 parts of plantain, and 5 to 10 parts of senna. The Chinese medicine compound has a significant effect in enhancing the survival rate of long-moire grouper offspring.

[0024] The mechanism of action of the Chinese medicine composition is very unique and comprehensive. Astragalus, as the main drug, can enhance the physique of the clouded grouper, improve its disease resistance, and lay a solid foundation for extending the spawning period. Honeysuckle and Phellodendron chinense work synergistically, have the effects of clearing away heat and detoxification, antibacterial and anti-inflammatory, and help maintain the reproductive health of the clouded grouper. The addition of Eucommia leaf powder and licorice can not only reconcile the various medicines, but also promote the balance of hormones in the clouded grouper, and further promote spawning. Hawthorn and Atractylodes can improve the digestive system of the clouded grouper, improve its nutrient absorption capacity, and provide sufficient energy reserves for spawning. Safflower and Acanthopanax have the effects of promoting blood circulation and removing blood stasis, enhancing the vitality of the body, and can improve the blood circulation of the clouded grouper, promote ovarian development, and thus extend the spawning period. Magnolia officinalis and Plantain can promote diuresis and reduce swelling, help the clouded grouper excrete excess water and toxins in the body, and keep the reproductive system clean and healthy. Finally, the addition of senna leaves has the effect of moistening the intestines and promoting bowel movements, helping the moire grouper to excrete waste and toxins from the body and maintain a healthy state of the body. In summary, the Chinese herbal compound acts on the moire grouper through multiple mechanisms of action, thereby achieving the effect of extending the spawning period and improving the survival rate of offspring.

[0025] The beneficial effects of the present invention are:

[0026] The present invention proposes an innovative method, which successfully extends the breeding period of the parent Epinephelus broodstock from the original one month to more than five months by artificially regulating the aquaculture water temperature of the "Yunlong Grouper" mother Epinephelus broodstock during the seedling and adult stages, the light cycle during the parent fish cultivation period, the induction of exogenous hormones, and Chinese medicine conditioning. This breakthrough allows the parent fish of Epinephelus broodstock to spawn multiple times within a year, significantly increasing their egg production. As the frequency of parent fish spawning increases, the yield of fertilized eggs also increases, which greatly promotes the scale and industrial development of the "Yunlong Grouper" seedlings.

[0027] In the specific implementation process of the present invention, the effective promotion of the gonadal development of the long-moire grouper broodstock is achieved through the carefully designed coordinated control strategy of the light cycle and water temperature. Specifically, 1 to 2 months before the broodstock spawn, the culture water temperature is gradually reduced from 26°C to 20°C, and the daylight time is controlled to about 12 hours. After one month of adjustment, the culture water temperature is gradually raised to 25°C, and the daylight time is gradually increased to 13.5 hours. This scientific control method effectively promotes the development of the broodstock gonads, thereby achieving the goal of multiple spawning of the moire grouper broodstock within a year. Through this method, not only the breeding efficiency of the moire grouper is improved, but also solid technical support is provided for the large-scale production of "Yunlong grouper" seedlings. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] A method for extending the spawning period of Epinephelus mole, comprising the following steps:

[0031] (1) Young Epinephelus moire with a body length of 20 to 25 cm and a body weight of 100 to 150 g were selected as broodstock for breeding. The breeding was carried out in a factory-based breeding environment under the following conditions: dissolved oxygen 8 mg / L, salinity 30, pH 8, and water exchange volume 2.5 m 3 / h, water temperature 26 ℃, daylight time is 13.5h, and breeding density is controlled at 7kg / m3; feed 1 is fed once a day, and the feeding amount is based on the fact that the broodstock no longer compete for food. At the same time, the Chinese medicine composition is added to feed 1 for 10 days each month and fed together.

[0032] (2) When the broodstock reach sexual maturity, starting from December of the same year, 1.5 months before spawning, the culture water temperature is lowered to 20°C, the daylight duration is controlled to 12 h, and the culture density is controlled to 7 kg / m3; feeds 2 and 3 are fed once a day until the broodstock no longer compete for food. At the same time, the Chinese medicine composition is added to feeds 2 and 3 for 10 days each month and fed together; after 1 month, the culture water temperature is gradually increased to 25°C, and the daylight duration is gradually increased to 13.5 h. The regulation time is 1 month to promote the gonadal development of the broodstock.

[0033] (3) When the gonads of the broodstock develop to stage 3-4, chorionic gonadotropin is injected to induce spawning. The fertilized eggs of "Yunlong grouper" are obtained through artificial insemination, which can be 2 months earlier than the breeding time of ordinary broodstock. The method of artificial insemination is: artificially squeeze the abdomen of the female clouded grouper that has fully expanded after artificial induction, and the mature eggs flow out of the cloaca. Collect them in a beaker or a small basin; gently squeeze the abdomen of the male saddle grouper in the same way to make the semen flow out of the cloaca. Use a straw to absorb the semen or use a small beaker to collect the semen; add the collected saddle grouper semen to the clouded grouper eggs. Generally, add about 0.2 ml of semen to 1 kg of fish eggs. Gently stir with fingers or goose feathers to fully mix the sperm and eggs. Add an appropriate amount of fresh filtered seawater for about 1 minute. After 2-3 minutes, the sperm and eggs are fertilized.

[0034] (4) Multiple batches of spawning by broodstock: 30 days after a batch of broodstock spawns, the water temperature for broodstock cultivation is gradually reduced from 25°C to 20°C, and the light duration is gradually reduced from 13 hours and 30 minutes to 12 hours; in the next 30 days, the water temperature for broodstock cultivation is gradually increased from 20°C to 25°C, and the light duration is gradually increased from 12 hours to 13 hours and 30 minutes, so that the gonads of the broodstock redevelop and mature, and the second spawning is achieved. This process is repeated, and a batch of broodstock can spawn once every 2 months.

[0035] Feed 1: 35 parts of fish meal, 15 parts of corn flour, 8 parts of soybean meal, 8 parts of rice bran, 4 parts of fish oil, 4 parts of enteromorpha, 12 parts of α-starch, 2 parts of soybean lecithin, 15 parts of artemia powder, 0.4 parts of chitosan, 12 parts of Antarctic krill powder, 1 part of vitamin mixture (accounting for the weight of the whole feed), 1 part of mineral mixture, 0.03 parts of compound microecological preparation. The feed is in soft granular form with a particle size of 3 to 5 mm.

[0036] Feed 2: 45 parts of fish meal, 15 parts of corn flour, 8 parts of soybean meal, 8 parts of rice bran, 4 parts of fish oil, 4 parts of enteromorpha, 12 parts of α-starch, 2 parts of soybean lecithin, 8 parts of artemia powder, 0.4 parts of chitosan, 12 parts of Antarctic krill powder, 1 part of vitamin mixture, 1 part of mineral mixture, 0.03 parts of compound microecological preparation, 0.04 parts of VE. The feed is made into soft granules with a particle size of 10-20 mm.

[0037] Feed 3: 1 portion of fresh shrimp meat and 1 portion of fresh squid.

[0038] The components of the vitamin mixture, calculated by weight of the vitamin mixture, include 3.2 parts of vitamin A, 0.5 parts of vitamin D3, 1 part of vitamin K, 2 parts of vitamin C, 2.6 parts of vitamin B1 (thiamine), 0.01 parts of vitamin B2 (riboflavin), 20.2 parts of vitamin B3 (niacin), 6.2 parts of vitamin B5 (pantothenic acid), 2 parts of vitamin B6 (pyridoxine), 2 parts of vitamin B9 (folic acid), 0.5 parts of vitamin B12 (cobalamin), 0.12 parts of biotin, etc.

[0039] The components of the mineral mixture, calculated by weight of the mineral mixture, include the following major elements: 40 parts of calcium (Ca), 10 parts of phosphorus (P), 20 parts of sodium (Na), 20 parts of potassium (K), 3 parts of magnesium (Mg), 15 parts of chlorine (Cl), 10 parts of sulfur (S) and trace elements: 0.04 parts of iron (Fe), 0.02 parts of copper (Cu), 0.01 parts of zinc (Zn), 0.03 parts of cobalt (Co), 0.002 parts of iodine (I), 0.001 parts of selenium (Se) and 0.003 parts of manganese (Mn).

[0040] The Chinese medicine composition comprises: 18 parts of astragalus, 12 parts of honeysuckle, 8 parts of phellodendron, 12 parts of eucommia leaf powder, 12 parts of liquorice, 12 parts of hawthorn, 8 parts of atractylodes, 8 parts of safflower, 12 parts of acanthopanax, 8 parts of magnolia bark, 12 parts of plantain, and 8 parts of senna.

[0041] The composite microecological preparation is composed of lactobacillus, bacillus subtilis and yeast in a mass ratio of 1:1:1, and the content of the mixed bacteria is 1 to 1.5×10 8 cfu / g.

[0042] The injection dosage of the chorionic gonadotropin is 800 μg / kg fish body.

[0043] The amount of the Chinese medicine composition added to feed is 0.15wt%.

[0044] According to the water temperature of the clouded grouper fry breeding area, a batch of broodstock can be matured and spawned every month. High-quality fertilized eggs can be obtained from February to June every year. The breeding season is extended from the original one month to more than five months.

[0045] Comparative Example 1

[0046] The difference from Example 1 is that the water temperature is constantly controlled at 26°C (i.e., the following operation is not performed: 1.5 months before spawning, the culture water temperature is lowered to 20°C, and 1 month later, the culture water temperature is gradually raised to 25°C), and other operations are the same as Example 1.

[0047] Comparative Example 2

[0048] The difference from Example 1 is that the water temperature is constantly controlled at 24°C (i.e., the following operation is not performed: 1.5 months before spawning, the culture water temperature is lowered to 20°C, and 1 month later, the culture water temperature is gradually raised to 25°C), and other operations are the same as Example 1.

[0049] Comparative Example 3

[0050] The difference from Example 1 is that in step (2), after one month, the culture water temperature is gradually increased to 30°C (that is, 1.5 months before spawning, the culture water temperature is reduced to 20°C, and after one month, the culture water temperature is gradually increased to 30°C). Other operations are the same as in Example 1. The experimental results are shown in Table 1.

[0051] Comparative Example 4

[0052] The difference from Example 1 is that the Chinese medicine composition does not contain Astragalus, Honeysuckle and Phellodendron, and the other operations are the same as Example 1.

[0053] Comparative Example 5

[0054] The difference from Example 1 is that the Chinese medicine composition does not contain Eucommia leaf powder, licorice and hawthorn, and the other operations are the same as Example 1.

[0055] Comparative Example 6

[0056] The difference from Example 1 is that the Chinese medicine composition does not contain Atractylodes macrocephala, Carthamus tinctorius, and Acanthopanax senticosus, and the other operations are the same as Example 1.

[0057] Comparative Example 7

[0058] The difference from Example 1 is that the Chinese medicine composition does not contain Magnolia officinalis, Plantago asiatica and Senna leaves, and the other operations are the same as Example 1.

[0059] Comparative Example 8

[0060] The difference from Example 1 is that in step (2), the culture water temperature is maintained at 25°C (1.5 months before spawning, the culture water temperature is lowered to 25°C, and after 1 month, the culture water temperature is gradually raised to 25°C), the daylight exposure time is 12 hours, and other operations are the same as Example 1.

[0061] Comparative Example 9

[0062] The difference from Example 1 is that the feed does not contain a composite microecological preparation, and other operations are the same as Example 1.

[0063] The data of egg laying amount, hatched number, hatching rate, survival rate and the like in February, April and June of Example 1 and Comparative Examples 1 to 10 were respectively counted, and the experimental results are shown in Table 1.

[0064] Among them, the spawning volume is: the weight of eggs squeezed out by mature female fish through artificial assistance during artificial insemination, and the number of eggs per kilogram of clouded grouper is about 1.6 million; hatching rate = number of hatched fry / number of fertilized eggs*100%; 7-day survival rate = number of fry on the 7th day of cultivation / number of fry on the 1st day of hatching*100%.

[0065] In both Example 1 and Comparative Examples 1-9, 25 broodstock were counted.

[0066] According to data analysis, we can draw the following conclusions: As can be seen from Table 1, in Example 1, the culture water temperature was gradually reduced from 26°C to 20°C 1.5 months before the spawning of the broodstock, and after 1 month, the culture water temperature was gradually increased to 25°C. Through this temperature control, combined with the reasonable arrangement of the light exposure time, as well as comprehensive methods such as the induction of exogenous hormones and Chinese medicine conditioning, the development of the gonads of the broodstock can be effectively promoted, thereby achieving the goal of spawning three times in a year. This shows that the synergistic effect of temperature control and light exposure time, as well as the combination of exogenous hormones and Chinese medicine conditioning, has a significant promoting effect on the multiple spawning of the broodstock of the long cloud pattern grouper.

[0067] In contrast, the broodstock in Comparative Examples 1 to 3 and Comparative Example 8 were not subjected to the above-mentioned coordinated treatment of temperature regulation and light exposure time, and the results showed that these broodstock could only lay eggs once a year. This further confirms the effectiveness of the coordinated treatment of temperature regulation and light exposure time in Example 1.

[0068] In Comparative Examples 4 to 7, although the coordinated treatment of temperature control and light exposure time is the same as that in Example 1, the components of the Chinese medicine composition are different from those in Example 1. The experimental results show that these broodstock can also lay eggs three times a year, but their offspring survival rate within 7 days is significantly lower than that in Example 1. This result shows that the Chinese medicine composition used in Example 1 has a significant advantage in improving the survival rate of offspring of long clouded grouper.

[0069] Finally, in Comparative Example 9, the main difference from Example 1 is that no composite probiotic preparation was added to the feed. Despite this, these broodstock can lay eggs three times a year, but the hatching rate of their fry is significantly lower than that of Example 1. This result further proves the effectiveness of the composite probiotic preparation used in Example 1 in improving the hatching rate of long clouded grouper fry.

[0070] Table 1

[0071]

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for extending the spawning period of Epinephelus moire, characterized in that: The following steps are involved: (1) Young Epinephelus moire with a body length of 20-25 cm and a body weight of 100-150 g were selected as broodstock for breeding. The breeding was carried out in a factory-based breeding environment under the following conditions: dissolved oxygen 5-9 mg / L, salinity 25-33, pH 7.8-8.2, and water flow 2-3 m 3 / h, water temperature 25-28℃, daylight duration 13.5h, cultivation density controlled at 6-8kg / m 3 ; Feed 1 once a day, the feeding amount is based on the broodstock no longer competing for food. At the same time, the Chinese medicine composition is added to Feed 1 for 10 days each month; (2) When the broodstock reach sexual maturity, starting from December each year, 1 to 2 months before spawning, the water temperature is lowered to 20°C, the daylight duration is 12 hours, and the breeding density is 6 to 8 kg / m 3 Feed 2 and 3 were fed once a day until the broodstock stopped competing for food. At the same time, the Chinese medicine composition was added to feed 2 and 3 for 10 days each month. After 1 month, the culture water temperature was gradually increased to 25°C, and the daylight duration was gradually increased to 13.5h. (3) When the gonads of the broodstock develop to stage 3-4, chorionic gonadotropin or luteinizing hormone-releasing hormone analogs are injected to induce spawning; (4) 30 days after the broodstock spawn, the water temperature is gradually reduced to 20°C, and the light duration is gradually reduced to 12 hours; 30 days later, the water temperature is gradually increased to 25°C, and the light duration is gradually increased to 13.5 hours, so that the gonads of the broodstock develop and mature again, and the second spawning can be achieved; this process is repeated, and a batch of broodstock can spawn once in 2 months; Feed 1: 30-40 parts of fish meal, 10-20 parts of corn flour, 5-10 parts of soybean meal, 5-10 parts of rice bran, 3-5 parts of fish oil, 3-5 parts of enteromorpha, 10-15 parts of α-starch, 1.5-2.5 parts of soybean lecithin, 10-20 parts of artemia powder, 0.3-0.5 parts of chitosan, 10-15 parts of Antarctic krill powder, 1 part of vitamin mixture, 1 part of mineral mixture, 0.03-0.04 parts of compound microecological preparation. The feed is in soft granular form with a particle size of 3-5 mm. Feed 2: 40-50 parts of fish meal, 10-20 parts of corn flour, 5-10 parts of soybean meal, 5-10 parts of rice bran, 3-5 parts of fish oil, 3-5 parts of enteromorpha, 10-15 parts of α-starch, 1.5-2.5 parts of soybean lecithin, 5-10 parts of artemia powder, 0.3-0.5 parts of chitosan, 10-15 parts of Antarctic krill powder, 1 part of vitamin mixture, 1 part of mineral mixture, 0.03-0.04 parts of compound microecological preparation, 0.04-0.05 parts of VE. The feed is made into soft granules with a particle size of 10-20 mm. Feed 3: 1 portion of fresh shrimp meat, 1 portion of fresh squid; The Chinese medicine composition comprises: 15-20 parts of astragalus, 10-15 parts of honeysuckle, 5-10 parts of phellodendron, 10-15 parts of eucommia leaf powder, 10-15 parts of liquorice, 10-15 parts of hawthorn, 5-10 parts of atractylodes, 5-10 parts of safflower, 10-15 parts of acanthopanax, 5-10 parts of magnolia bark, 10-15 parts of plantain, and 5-10 parts of senna leaves; The composite microecological preparation is composed of lactobacillus, bacillus subtilis, and yeast in a mass ratio of 1:1:1, and the content of the mixed bacteria is 1×10 7 cfu / g~1×10 9 cfu / g.

2. The method for prolonging the spawning period of Epinephelus moire according to claim 1, characterized in that: The injection dosage of the chorionic gonadotropin is 500-1000 μg / kg fish body.

3. The method for prolonging the spawning period of Epinephelus moire according to claim 1, characterized in that: The injection dosage of the luteinizing hormone releasing hormone analogue is 2-5 μg / kg fish body.

4. The method for prolonging the spawning period of Epinephelus moire according to claim 1, characterized in that: The amount of the Chinese medicine composition added to feed is 0.1-0.2 wt%.

5. A Chinese medicinal composition for prolonging the spawning period of Epinephelus moire, characterized in that: The invention comprises the following components: 15-20 parts of astragalus, 10-15 parts of honeysuckle, 5-10 parts of phellodendron, 10-15 parts of eucommia leaf powder, 10-15 parts of licorice, 10-15 parts of hawthorn, 5-10 parts of atractylodes, 5-10 parts of safflower, 10-15 parts of acanthopanax, 5-10 parts of magnolia bark, 10-15 parts of plantain and 5-10 parts of senna.

6. The Chinese medicinal composition for prolonging the spawning period of Epinephelus moire according to claim 5, characterized in that: The invention comprises the following components: 12 parts of astragalus, 8 parts of honeysuckle, 8 parts of phellodendron, 12 parts of eucommia leaf powder, 12 parts of liquorice, 12 parts of hawthorn, 8 parts of atractylodes, 8 parts of safflower, 12 parts of acanthopanax, 8 parts of magnolia bark, 12 parts of plantain and 8 parts of senna.

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