A composite plant growth regulator, its preparation method and application

Abstract

The present invention discloses a composite plant growth regulator, its preparation method, and application. The composite plant growth regulator comprises 10-20 parts of an active ingredient, 80-100 parts of a solvent, and 8-10 parts of a promoter. The active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:300-400; the solvent is water. The composite plant growth regulator of the present invention combines the active ingredient and the promoter in an appropriate ratio to significantly promote wheat growth and development, enhance plant photosynthesis, improve crop lodging resistance, and ultimately increase wheat yield and quality. It also effectively reduces the use of chemically synthesized growth regulators, is safer and more environmentally friendly, and has a low risk of drug resistance.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and in particular relates to a composite plant growth regulator and a preparation method and application thereof. Background Art

[0002] Wheat is one of my country's important grain crops, playing a crucial role in national food security and economic development. The discovery and application of dwarfing genes in the latter half of the 20th century not only reduced wheat's susceptibility to lodging but also improved its harvest index and yield. Modern breeding techniques can further reduce wheat plant height. However, while this further reduction in plant height improves lodging resistance, it also leads to smaller leaves, poor field ventilation and light transmission, and compromises crop photosynthesis, leading to lower yields. Therefore, reducing wheat lodging and achieving stable yields under high- and super-high-yield conditions is a challenge. Lodging is closely related to the wheat growing environment, management practices, growth and development processes, and cultivar characteristics. The use of growth regulators is one effective way to improve crop lodging resistance. Currently, the main types of plant growth regulators used to improve crop lodging resistance are chemically synthesized growth regulators, which fall into two main categories: gibberellin synthesis inhibitors, such as paclobutrazol, and ethylene releasers, such as ethephon. Although chemically synthesized growth regulators are effective in improving wheat's lodging resistance, their long-term use may have potential impacts on the environment and human health. For example, they may leave residues in the soil and affect soil microbial communities. Long-term use of the same chemical regulator may lead to drug resistance in plants or pathogens, reducing the regulator's effectiveness. It may also have a negative impact on non-target organisms (such as beneficial insects), disrupting the ecological balance. Plant-derived growth regulators have significant advantages in applications such as wheat lodging resistance, such as being environmentally friendly, safe, having a low risk of drug resistance, being safe for beneficial organisms, being multifunctional, and being eco-friendly. They are ideal alternatives to chemically controlled regulators. Therefore, how to prepare a safer and more stable composite plant growth regulator has become a technical problem that needs to be solved urgently. Summary of the Invention

[0003] The present invention aims to provide a composite plant growth regulator, which can significantly promote wheat growth and development, enhance plant photosynthesis, improve crop lodging resistance, and ultimately improve wheat yield and quality when used in wheat cultivation.

[0004] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0005] A composite plant growth regulator is composed of 10-20 parts of an effective active ingredient, 80-100 parts of a solvent, and 8-10 parts of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:300-400; and the solvent is water.

[0006] Preferably, the accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2-0.5:1.

[0007] Preferably, the cuttlebone extract is prepared by the following method:

[0008] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water according to the material-liquid ratio, adjust the pH of the solution to 5.5-6.0, add 1.5wt% complex enzyme solution, hydrolyze at 50℃ for 2h, increase the temperature to 55℃ and adjust the pH to 6.5-7.5, then add 3wt% pectinase, hydrolyze for 2h, then add 1wt% chitosanase and hydrolyze at 40-45℃ for 3-4h, inactivate the enzyme at high temperature to obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0009] (2) Residue A was mixed with 80% ethanol according to the material-liquid ratio, and then ultrasonically dispersed for 30 minutes, heated at 60°C with stirring and reflux for 1 hour, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 minutes, and the supernatant was obtained to obtain supernatant B;

[0010] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0011] Preferably, in step (1), the material-liquid ratio of cuttlebone powder to deionized water is 1 g:30 ml.

[0012] Preferably, the complex enzyme solution in step (1) contains 5-10 g / L cellulase and 10-15 g / L flavor protease.

[0013] Preferably, the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0014] Preferably, the enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0015] Preferably, the material-liquid ratio of the residue A to 80% ethanol in step (2) is 1 g:10 ml.

[0016] The present invention also provides a method for preparing the above-mentioned composite plant growth regulator, comprising the following steps:

[0017] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0018] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0019] The composite plant growth regulator prepared by the present invention can be used to promote wheat growth and improve its lodging resistance. It needs to be diluted 8-10 times before spraying, with a dosage of 10 L per mu. It can be sprayed during various growth stages, including the tillering, jointing, booting, and grain filling stages.

[0020] The cuttlebone extract prepared by the present invention utilizes multiple enzymes to perform decomposition under different conditions, thereby maximally decomposing the components in the cuttlebone. The resulting product is rich in multiple small-molecule bioactive components, such as polysaccharides, amino acids, organic acids, etc. The multiple active components work together to promote nutrient absorption and utilization, promote wheat root growth, enhance the plant's anchoring ability, and simultaneously enhance the plant's tolerance to environmental stress. In addition, the calcium salt component in the cuttlebone extract can enhance the stability of plant cell walls, promote the thickening and strengthening of cell walls, make the wheat stems thicker, and dwarf the plants, thereby effectively improving the wheat's lodging resistance.

[0021] Brassinolide is a natural plant growth regulator that significantly promotes plant growth and development, improves photosynthesis efficiency, and enhances plant resistance to stress. Combining brassinolide with cuttlebone extract creates a synergistic effect, further enhancing wheat's resistance to lodging. Brassinolide regulates endogenous plant hormones, promoting cell wall thickening and stronger stems. The active ingredients in cuttlebone extract further modulate the plant's immune response and enhance its overall health. This combination significantly improves wheat's resistance to stress, enabling it to maintain optimal growth in adverse environmental conditions such as drought and salinity.

[0022] The accelerator used in this invention contains two components: taurine and methyltriethoxysilane. Taurine improves the permeability of plant cell membranes, facilitating plant absorption of brassinolide and cuttlebone extract. It also regulates biochemical reactions within the plant, enhancing its ability to adapt to adverse conditions. Methyltriethoxysilane and taurine act synergistically, further enhancing plant cell membrane permeability and the regulator's ability to penetrate the leaves. This helps brassinolide and cuttlebone extract enter the plant more quickly to take effect, improving their absorption efficiency. The two components complement each other and are indispensable.

[0023] The beneficial effects of the present invention are: the prepared composite plant growth regulator has a significant effect on improving the lodging resistance of wheat plants in wheat cultivation, can significantly inhibit the elongation of wheat internodes, dwarf plant height, make seedlings uniform and strong, and improve the lodging resistance of plants; increase the photosynthesis rate of leaves, promote the development of wheat ears, and improve wheat yield and quality; effectively reduce the use of chemical synthetic growth regulators, are safer and more environmentally friendly, and have a low risk of drug resistance. DETAILED DESCRIPTION

[0024] The technical solution of the present invention will be further described below with reference to specific embodiments, but is not limited thereto.

[0025] Example 1

[0026] A composite plant growth regulator is composed of 10 kg of an effective active ingredient, 80 kg of a solvent, and 8 kg of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:300; and the solvent is water.

[0027] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0028] The cuttlebone extract is prepared by the following method:

[0029] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0030] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0031] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0032] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0033] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0034] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0035] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0036] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0037] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0038] Example 2

[0039] A composite plant growth regulator is composed of 20 kg of an effective active ingredient, 100 kg of a solvent, and 10 kg of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:350. The solvent is water.

[0040] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.4:1.

[0041] The cuttlebone extract is prepared by the following method:

[0042] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 6.0, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 7.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 45 ° C for 4 h, inactivate the enzyme at high temperature, obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0043] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0044] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0045] The complex enzyme solution in step (1) contains 10 g / L cellulase and 15 g / L flavor protease.

[0046] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0047] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0048] The present invention also provides a method for preparing the above-mentioned composite plant growth regulator, comprising the following steps:

[0049] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0050] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0051] Example 3

[0052] A composite plant growth regulator is composed of 15 kg of effective active ingredients, 90 kg of solvent, and 9 kg of promoter. The effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:400; and the solvent is water.

[0053] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.5:1.

[0054] The cuttlebone extract is prepared by the following method:

[0055] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.8, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 7.0, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 45 ° C for 4 h, inactivate the enzyme at high temperature, obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0056] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0057] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0058] The complex enzyme solution in step (1) contains 8 g / L cellulase and 12 g / L flavor protease.

[0059] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0060] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0061] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0062] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0063] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0064] Comparative Example 1

[0065] A composite plant growth regulator comprises 10 kg of an effective active ingredient and 80 kg of a solvent; the effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:300; and the solvent is water.

[0066] The cuttlebone extract is prepared by the following method:

[0067] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0068] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0069] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0070] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0071] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0072] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0073] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0074] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0075] S2: Take a solvent and then add the effective active ingredient obtained in step S1 into it, high-pressure homogenize for 3-5 minutes, sterilize and then package to obtain the final product.

[0076] This comparative example is basically the same as Example 1, except that it does not contain the accelerator component.

[0077] Comparative Example 2

[0078] A composite plant growth regulator is composed of 10 kg of brassinolide, 80 kg of water, and 8 kg of an accelerator, wherein the accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0079] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0080] S1: Add brassinolide and accelerator to the solvent, stir evenly, and high-pressure homogenize for 3-5 minutes. After sterilization, divide the package to obtain the final product.

[0081] This comparative example is basically the same as Example 1, except that it does not contain the cuttlebone extract and its preparation method.

[0082] Comparative Example 3

[0083] A composite plant growth regulator is provided, which is composed of 10 kg of cuttlebone extract, 80 kg of water, and 8 kg of a promoter, wherein the promoter is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0084] The cuttlebone extract is prepared by the following method:

[0085] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0086] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0087] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0088] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0089] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0090] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0091] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0092] S1: Preparation of cuttlebone extract;

[0093] S2: Add the accelerator to the solvent, stir evenly, then add the cuttlebone extract obtained above, homogenize under high pressure for 3-5 minutes, sterilize and then package to obtain the final product.

[0094] This comparative example is basically the same as Example 1, except that it does not contain brassinolide.

[0095] Comparative Example 4

[0096] A composite plant growth regulator is composed of 10 kg of an effective active ingredient, 80 kg of a solvent, and 8 kg of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:100; and the solvent is water.

[0097] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0098] The cuttlebone extract is prepared by the following method:

[0099] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0100] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0101] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0102] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0103] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0104] The enzyme activity of the pectinase is 50,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0105] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0106] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0107] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0108] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0109] Comparative Example 5

[0110] A composite plant growth regulator is composed of 10 kg of an effective active ingredient, 80 kg of a solvent, and 8 kg of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:200; and the solvent is water.

[0111] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0112] The cuttlebone extract is prepared by the following method:

[0113] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0114] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0115] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0116] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0117] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0118] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0119] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0120] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0121] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0122] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0123] Comparative Example 6

[0124] A composite plant growth regulator is composed of 10 kg of an effective active ingredient, 80 kg of a solvent, and 8 kg of a promoter. The effective active ingredient is brassinolide and a cuttlebone extract in a mass ratio of 1:500; and the solvent is water.

[0125] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0126] The cuttlebone extract is prepared by the following method:

[0127] (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a material-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% complex enzyme solution, hydrolyze at 50 ° C for 2 h, increase the temperature to 55 ° C and adjust the pH to 6.5, then add 3 wt% pectinase and hydrolyze for 2 h, then add 1 wt% chitosanase and hydrolyze at 40 ° C for 3 h, inactivate the enzyme at high temperature, obtain the hydrolyzate, and separate by high-speed centrifugation to obtain supernatant A and residue A;

[0128] (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60°C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B.

[0129] (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

[0130] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0131] The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0132] The enzyme activity of the pectinase is 50,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0133] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps:

[0134] S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient;

[0135] S2: adding the accelerator to the solvent and stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0136] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0137] Wheat planting experiment

[0138] The experimental field was selected from a farmland in Qingyun Town, Linshu County, Linyi City. The average annual temperature is 14.7°C, the average annual precipitation is 866.8 mm, and the soil is clay-alkaline with above-average fertility. The soil pH was 7.60, the organic matter content was 21.51 g / kg, the total nitrogen content was 1.35 g / kg, the available phosphorus content was 10.66 mg / kg, the available potassium content was 175.21 mg / kg, and the total salt content was 1.42 g / kg. The previous crop was rice. The wheat variety used for the experiment was Linmai 14, obtained from the Linyi Academy of Agricultural Sciences. Wheat was sown mechanically in rows, with a sowing rate of 15.0 kg per mu and a basic seedling number of about 220,000 plants per mu. Base fertilizer was 40.0 kg of compound fertilizer (N:P:K=15%:15%:15%, mass fraction, the same below) per mu, and 5 kg / mu of urea (N content of 46%) was applied as tillering fertilizer. 5 kg / mu of urea (N content of 46%) was applied as jointing fertilizer and heading fertilizer respectively.

[0139] The experiment set up 10 treatments, namely Examples 1-3 and Comparative Examples 1-6 and a blank control test group, and each treatment area was 1 mu. The composite plant growth regulator was applied by drone, and the leaves were sprayed once at the tillering stage, jointing stage, and heading stage of wheat, with a dosage of 5L / mu, diluted 10 times and then sprayed; the blank control group was not sprayed with any product. 30 days after the first application of the growth regulator, the wheat seedlings were investigated, and each treatment group adopted a diagonal 5-point sampling method, with an area of 1m 2 The number of tillers was recorded. During the wheat maturity stage, a five-point diagonal sampling method was used for each treatment, with 20 representative wheat plants selected at each point (a total of 100 plants per treatment). The length of the first and second internodes at the base, stem diameter, fresh weight, and dry weight were measured, and the bending strength of the second internode was measured. In addition, the ear length and plant height of each treatment were measured, and lodging in the field was observed. The test results are shown in Tables 1-3.

[0140] Table 1 Effects of different treatments with plant growth regulators on wheat traits

[0141]

[0142] As can be seen from the contents of Table 1 above, compared with the blank control group, the composite plant growth regulator prepared in Examples 1-3 of the present invention can significantly promote wheat tillering, and the length of the first and second internodes at the base of wheat is reduced, and the stem thickness is increased. This shows that the regulator of the present invention can significantly inhibit the elongation of wheat internodes, dwarf plant height, optimize nutrient supply to increase the stem thickness of wheat, and improve the lodging resistance of the plant.

[0143] Table 2 Effects of different treatments with plant growth regulators on fresh and dry weight of wheat internodes

[0144]

[0145] It can be seen from the contents of Table 2 above that, compared with the blank group and the control group, the use of the composite plant growth regulators of Examples 1-3 of the present invention promoted the accumulation of dry matter in wheat internodes.

[0146] Table 3 Effects of different treatments on wheat spike length, plant height and lodging resistance

[0147]

[0148] As can be seen from the data in Table 3 above, compared with the blank control group, the plant height and lodging rate of the treatment group of Examples 1-3 of the present invention were significantly reduced, and the spike length increased, which shows that the use of the composite plant growth regulator of the present invention can dwarf the plant, promote the development of wheat spikes by regulating the plant nutrient absorption balance, and achieve the purpose of strong seedlings and promoting production. At the same time, the multi-component joint action of the present invention can also promote the growth of wheat root system, enhance the anchoring ability of the plant, and dwarf the plant, thereby effectively improving the lodging resistance of wheat. As can be seen from the data in Comparative Examples 1-6, by changing the dosage composition and ratio of the raw materials of the present invention, the corresponding growth traits of the wheat plants weaken and the lodging resistance deteriorates.

[0149] Wheat was harvested at maturity, and the wheat yield and yield composition were calculated. The specific results are shown in Table 4.

[0150] Table 4 Effects of different treatment groups on wheat yield and yield components

[0151]

[0152] As can be seen from Table 4, the number of wheat ears, number of grains per ear, per-acre weight, and yield per mu after the composite plant growth regulator of Examples 1-3 of the present invention were significantly higher than those of the blank control group. While the yield of the treatment groups of Comparative Examples 1-6 also increased, the overall effect was lower than that of the Examples. This indicates that the formula ratio of the Examples of the present invention is the optimal dosage ratio. Changing the ratio or composition of the raw materials will reduce the growth-promoting effect on wheat.

[0153] It should be noted that the above embodiments are only some of the preferred embodiments of the present invention, and not all of them. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present invention.

Claims

1. A composite plant growth regulator, which is composed of 10-20 parts of effective active ingredients, 80-100 parts of solvents, and 8-10 parts of promoters, characterized in that: The effective active ingredient is brassinolide and cuttlebone extract in a mass ratio of 1:300-400; the solvent is water; and the accelerator is taurine and methyltriethoxysilane in a mass ratio of 0.2-0.5:

1. The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it, grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water according to the material-liquid ratio, adjust the pH of the solution to 5.5-6.0, add 1.5wt% complex enzyme solution, hydrolyze at 50℃ for 2h, increase the temperature to 55℃ and adjust the pH to 6.5-7.5, then add 3wt% pectinase, hydrolyze for 2h, then add 1wt% chitosanase and hydrolyze at 40-45℃ for 3-4h, inactivate the enzyme at high temperature to obtain enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; the complex enzyme solution contains 5-10g / L cellulase and 10-15g / L flavor protease; (2) Residue A was mixed with 80% ethanol according to the material-liquid ratio, and then ultrasonically dispersed for 30 minutes, heated at 60°C with stirring and reflux for 1 hour, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 minutes, and the supernatant was obtained to obtain supernatant B; (3) Supernatant A and supernatant B are mixed evenly to obtain cuttlebone extract.

2. The composite plant growth regulator according to claim 1, characterized in that In step (1), the material-liquid ratio of cuttlebone powder to deionized water is 1 g:30 ml.

3. The composite plant growth regulator according to claim 1, characterized in that The enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

4. The composite plant growth regulator according to claim 1, characterized in that The enzyme activity of pectinase is 40,000 U / g, and the enzyme activity of chitosanase is 100,000 U / g.

5. The composite plant growth regulator according to claim 1, characterized in that The material-liquid ratio of the residue A to 80% ethanol in step (2) is 1 g:10 ml.

6. A method for preparing the composite plant growth regulator according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: Add the accelerator to the solvent and stir evenly, then add the effective active ingredient obtained in step S1, homogenize under high pressure for 3-5 minutes, sterilize and then package to obtain the final product.

7. Use of the composite plant growth regulator according to any one of claims 1 to 5 for improving lodging resistance in wheat planting.

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