Nutritional composition beneficial to pet health and pet food

Abstract

The invention discloses a nutritional composition beneficial to pet health and pet food containing the nutritional composition. The nutritional composition comprises beta-glucan, plasma protein and lactoferrin. Wherein the mass ratio of the beta-glucan to the plasma protein to the lactoferrin is (0.055 to 0.275) to (1.56 to 4.68) to (0.00095 to 0.0285). The nutritional composition beneficial to pet health provided by the invention is used for improving intestinal health of pets and improving digestive ability; the immunity of the pets can be improved, and the hair health of the pets is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of pet feeds, and in particular to a nutritional composition beneficial to pet health and a pet food containing the nutritional composition. Background Art

[0002] In daily life, pets are becoming more and more important in people's hearts. How to make their pets grow healthily, meet the trace elements needed by pets through daily diet, increase the body's immunity and disease resistance, and reduce the occurrence of diseases are the topics that pet owners are more concerned about. However, for pets that often have various problems with gastrointestinal digestion and absorption, the food they feed usually has a great impact on the condition of the pets.

[0003] At present, pet food, pet nutritional supplements and other products have appeared on the market. Among them, pet food, as a nutritious food specially provided for cats and dogs, is a high-end pet food between human food and traditional livestock and poultry feed. Its main purpose is to provide pets with basic nutrients for life guarantee and growth and development. Pet nutritional supplements are a kind of dietary supplement products prepared with a variety of nutritional additives in a certain proportion, which can meet the needs of pets for nutrients such as amino acids, vitamins, mineral trace elements, enzyme preparations, etc.

[0004] For pet food, pet nutritional supplements and other products, they are required to have the characteristics of being able to supplement the nutrients needed for daily life activities, high digestion and absorption rate, scientific formula, and the ability to prevent cat and dog diseases. However, there are currently few types of nutritional supplements on the market that can improve pet health in many aspects, the combination is relatively simple, and the functions are not comprehensive enough. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a nutritional composition beneficial to the health of pets, which can improve the intestinal health of pets and enhance their digestive ability; it can also enhance the immunity of pets and improve the hair health of pets.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A first aspect of the present invention provides a nutritional composition beneficial to the health of pets, comprising β-glucan, plasma protein and lactoferrin; wherein, in the nutritional composition, the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.275): (1.56-4.68): (0.00095-0.0285).

[0008] β-Glucan is a polysaccharide substance, which is composed of glucose molecules connected by β-1,3-glycosidic bonds or β-1,6-glycosidic bonds. It is widely found in plants, fungi and bacteria. β-Glucan can inhibit inflammatory response, reduce inflammatory symptoms, and help alleviate the onset of inflammatory diseases. β-Glucan also has the effect of lowering blood sugar, which may work by repairing damaged pancreatic B cells and reducing exogenous glucose absorption. At the same time, it also has antioxidant, intestinal mucosal protection and lipid-lowering functions. In the field of skin care, β-Glucan can promote skin cell activity, achieve skin care effects, and also has super moisturizing properties and anti-inflammatory activity.

[0009] Plasma protein is the main solid component of plasma and is widely used in feed. Plasma protein is rich in a variety of essential amino acids and is easy to digest, which can supplement protein for pets. It also contains abundant immunoactive substances such as immunoglobulins, which can enhance the body's immune function and improve resistance to diseases. At the same time, plasma protein can also help maintain and repair the intestinal barrier, increase the tight junction of mucosal epithelium, improve intestinal barrier function, repair damaged epithelial cells, and reduce viral infection. In addition, plasma protein also has the effect of improving digestion and improving palatability.

[0010] Lactoferrin is an iron-binding protein found in breast milk and animal dairy products. It contains immune substances that can protect the body from infection by harmful microorganisms and has a significant effect on enhancing the body's disease resistance. It also has a certain antibacterial effect and can inhibit the growth of some bacteria. By binding to iron ions, it competes for the iron needed for bacterial reproduction, thereby achieving the effect of inhibiting bacterial reproduction. At the same time, lactoferrin can also promote the reproduction of bifidobacteria and lactic acid bacteria to maintain a healthy intestinal microecological environment.

[0011] The inventors found during the research that when the three components of β-glucan, plasma protein and lactoferrin are used together, a synergistic effect is unexpectedly produced, which significantly improves the health of pets, including improving the intestinal health of pets, improving the digestive ability of pets, improving the immunity of pets, and improving the hair health of pets. In addition, the inventors found that the three components of β-glucan, plasma protein and lactoferrin need to be compounded in a ratio of (0.055-0.275): (1.56-4.68): (0.00095-0.0285) to produce the above synergistic effect. If it is not within the above ratio range, the effect of improving the health of pets will be significantly reduced.

[0012] In the nutritional composition of the present invention, the mass ratio of beta-glucan, plasma protein and lactoferrin is (0.055-0.275):(1.56-4.68):(0.00095-0.0285), wherein within the ratio range, the value of beta-glucan can be 0.055, 0.1, 0.15, 0.2, 0.25, 0.275, etc., the value of plasma protein can be 1.56, 2, 2.5, 3, 3.5, 4, 4.5, 4.68, etc., and the value of lactoferrin can be 0.00095, 0.001, 0.002, 0.004, 0.005, 0.006, 0.008, 0.01, 0.015, 0.02, 0.025, 0.0285, etc.

[0013] In some preferred embodiments of the present invention, in the nutritional composition, the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.165): (1.56-3.12): (0.00095-0.019). Further preferably, the mass ratio of β-glucan, plasma protein and lactoferrin is 0.1485:2.34:0.00171.

[0014] In the present invention, the three raw materials of β-glucan raw material, porcine plasma protein powder and lactoferrin powder can be directly mixed evenly to obtain the nutritional composition beneficial to the health of pets. The β-glucan raw material, porcine plasma protein powder and lactoferrin powder can all be obtained from the market. Preferably, the purity of the active ingredient (β-glucan) in the β-glucan raw material is ≥55.0%, the purity of the active ingredient (plasma protein) in the porcine plasma protein powder is ≥78.0%, and the purity of the active ingredient (lactoferrin) in the lactoferrin powder is ≥95.0%.

[0015] The second aspect of the present invention provides the use of the composition in improving the intestinal health of pets and / or enhancing the immunity of pets and / or improving the hair health of pets.

[0016] The third aspect of the present invention provides use of the above-mentioned nutritional composition in preparing pet food.

[0017] A fourth aspect of the present invention provides a pet food, wherein the pet food comprises the above-mentioned nutritional composition.

[0018] The nutritional composition of the present invention can be used as a nutritional supplement for pets, which can exist alone in the form of a powder bag. When used, the powder bag is added to the raw material of pet food in a certain proportion to obtain pet food. In some embodiments, the amount of the nutritional composition added to the pet food can be 0.1-10wt%, for example, 0.1wt%, 0.2wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.8wt%, 1.0wt%, 2.0wt%, 3.0wt%, 4.0wt%, 5.0wt%, 6.0wt%, 7.0wt%, 8.0wt%, 9.0wt%, 10.0wt%, etc. Compared with the raw materials of pet food, after adding the nutritional composition, the nutritional components of pet food are more comprehensive, which can better improve the digestion and immunity of pets, and improve the intestinal health and hair health of pets.

[0019] In some preferred embodiments, the content of β-glucan in the pet food is 0.055-0.275wt%, for example, 0.055wt%, 0.1wt%, 0.15wt%, 0.2wt%, 0.25wt%, 0.275wt%, etc.; the content of plasma protein is 1.56-4.68wt%, for example, 1.56wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%. , 4.5wt%, 4.68wt%, etc.; the content of lactoferrin is 0.00095-0.0285wt%, for example, it can be 0.00095wt%, 0.001wt%, 0.002wt%, 0.004wt%, 0.005wt%, 0.006wt%, 0.008wt%, 0.01wt%, 0.015wt%, 0.02wt%, 0.025wt%, 0.0285wt%, etc. In a preferred embodiment, in the pet food, the content of β-glucan is 0.1485wt%, the content of plasma protein is 2.34wt%, and the content of lactoferrin is 0.00171wt%; in another preferred embodiment, in the pet food, the content of β-glucan is 0.1485wt%, the content of plasma protein is 2.34wt%, and the content of lactoferrin is 0.019wt%.

[0020] It should be noted that the above-mentioned pet food raw materials can be pet basic food (also known as base food), which contains some of the most basic nutrients needed by pets, such as protein, vitamins, minerals, and some oils and fats. For example, in some embodiments, the base food can include the following components by mass percentage: crude protein 30.0% to 50.0%, crude fat 9.0% to 25.0%, crude fiber ≤ 9%, moisture ≤ 14%, calcium 0.6% to 1.3%, total phosphorus 0.5% to 1.2%, taurine 0.1% to 0.2%. This type of base food can also be purchased commercially.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. In the nutritional composition beneficial to pet health provided by the present invention, the three components of β-glucan, plasma protein and lactoferrin produce a synergistic effect when compounded in a certain proportion, which significantly improves the intestinal health of pets, enhances the digestive ability and immune ability of pets, and improves the hair health of pets.

[0023] 2. The nutritional composition beneficial to pet health provided by the present invention can be applied to pet food and can improve the health of pets. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Provides a standard for grading pet cat hair;

[0025] Figure 2 The concentration graph of pet cat feces metabolites in the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0026] Figure 3 The concentration of immune factors in pet cat feces of the normal control group, the model control group, the Example 1 group and the comparative examples 1 to 6 groups;

[0027] Figure 4 This is a pet cat feces scoring diagram for the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0028] Figure 5 This is a pet cat hair grade scoring chart for the normal control group, model control group, Example 1 group, and Comparative Examples 1 to 6 groups;

[0029] Figure 6 This is a pet cat digestibility index diagram for the normal control group, the model control group, the Example 1 group, and the comparative examples 1 to 6 groups;

[0030] Figure 7 The concentration diagram of serum biochemical indexes of pet cats in normal control group, model control group, Example 1 group and Comparative Examples 1 to 6 groups;

[0031] Figure 8 The concentration of blood immune factors of pet cats in the normal control group, model control group, Example 1 group and Comparative Examples 1 to 6 groups;

[0032] Fig. 9 The concentration graph of pet cat feces metabolites in the normal control group, model control group, Example 1-4 groups and Comparative Example 7-8 groups;

[0033] Fig.10 It is a graph of immune factor concentrations in feces of pet cats of the normal control group, the model control group, Example 1 to 4 groups, and Comparative Example 7 to 8 groups;

[0034] Fig.11 This is a pet cat feces scoring diagram for the normal control group, model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0035] Fig.12 This is a pet cat hair grade scoring chart for the normal control group, model control group, Example 1 to 4 groups, and Comparative Example 7 to 8 groups;

[0036] Fig.13 This is a pet cat digestibility index diagram for the normal control group, the model control group, Example 1-4 groups, and Comparative Example 7-8 groups;

[0037] Fig.14 The concentration diagram of serum biochemical indexes of pet cats in the normal control group, model control group, Example 1 to 4 groups and Comparative Example 7 to 8 groups;

[0038] Fig.15 The following are the blood immune factor concentrations of pet cats in the normal control group, the model control group, Example 1-4 groups, and Comparative Example 7-8 groups. DETAILED DESCRIPTION

[0039] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings and embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0040] In the following examples and comparative examples, the purity of the β-glucan raw material is 55%, the purity of the porcine plasma protein powder is 78%, and the purity of the lactoferrin powder is 95%. The base food selected is the Let's Stay In cat food containing chicken, apples and lentils, and the component test results are shown in Table 1. Except for water and fat, all components are calculated on a dry basis.

[0041] Table 1

[0042]

[0043]

[0044] Example 1

[0045] The present embodiment provides a nutritional composition powder package beneficial to pet health, wherein the nutritional composition powder package is mixed with 0.27 parts of β-glucan raw material, 3 parts of porcine plasma protein powder and 0.0018 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.1485 parts of β-glucan, 2.34 parts of plasma protein and 0.00171 parts of lactoferrin.

[0046] 3.2718 g of the nutritional composition powder package of this embodiment was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.1485 wt% of β-glucan, 2.34 wt% of plasma protein, and 0.00171 wt% of lactoferrin.

[0047] Example 2

[0048] The present embodiment provides a nutritional composition powder package beneficial to pet health, wherein the nutritional composition powder package is mixed with 0.27 parts of β-glucan raw material, 3 parts of porcine plasma protein powder and 0.02 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.1485 parts of β-glucan, 2.34 parts of plasma protein and 0.019 parts of lactoferrin.

[0049] 3.29g of the nutritional composition powder package of this embodiment was added to the base food and mixed to obtain 100g of finished pet food. The content of active ingredients in the finished pet food was: 0.1485wt% of β-glucan, 2.34wt% of plasma protein, and 0.019wt% of lactoferrin.

[0050] Example 3

[0051] The present embodiment provides a nutritional composition powder package beneficial to pet health, wherein the nutritional composition powder package is mixed with 0.5 parts of β-glucan raw material, 2 parts of porcine plasma protein powder and 0.01 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.275 parts of β-glucan, 1.56 parts of plasma protein and 0.0095 parts of lactoferrin.

[0052] 2.51 g of the nutritional composition powder package of this embodiment was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.275 wt% of β-glucan, 1.56 wt% of plasma protein, and 0.0095 wt% of lactoferrin.

[0053] Example 4

[0054] The present embodiment provides a nutritional composition powder package beneficial to pet health, wherein the nutritional composition powder package is mixed with 0.1 parts of β-glucan raw material, 6 parts of porcine plasma protein powder and 0.03 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.055 parts of β-glucan, 4.68 parts of plasma protein and 0.0285 parts of lactoferrin.

[0055] 6.13 g of the nutritional composition powder package of this embodiment was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.055 wt% of β-glucan, 4.68 wt% of plasma protein, and 0.0285 wt% of lactoferrin.

[0056] Comparative Example 1

[0057] The comparative example provides a finished pet food, which is obtained by adding 0.27g of β-glucan raw material to 99.73g of base food and mixing. The content of active ingredients in the finished pet food is: 0.1485wt% of plasma protein.

[0058] Comparative Example 2

[0059] The comparative example provides a finished pet food, which is obtained by adding 3g of pig plasma protein powder to 97g of base food and mixing. The content of active ingredients in the finished pet food is: 2.34wt% of plasma protein.

[0060] Comparative Example 3

[0061] The comparative example provides a finished pet food, which is obtained by adding 0.0018g of lactoferrin powder to 99.9982g of base food and mixing. The content of active ingredients in the finished pet food is: 0.00171wt% of lactoferrin.

[0062] Comparative Example 4

[0063] The comparative example provides a nutritional composition powder package beneficial to pet health, which is prepared by mixing 0.27 parts of β-glucan raw material and 3 parts of pig plasma protein powder. The contents of active ingredients β-glucan and plasma protein in the nutritional composition powder package are 0.1485 parts of β-glucan and 2.34 parts of plasma protein, respectively.

[0064] 3.27 g of the nutritional composition powder package of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.1485 wt% of β-glucan and 2.34 wt% of plasma protein.

[0065] Comparative Example 5

[0066] The comparative example provides a nutritional composition powder package beneficial to pet health, which is prepared by mixing 0.27 parts of β-glucan raw material and 0.0018 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan and lactoferrin are 0.1485 parts of β-glucan and 0.00171 parts of lactoferrin, respectively.

[0067] 0.2718 g of the nutritional composition powder package of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.1485 wt% of β-glucan and 0.00171 wt% of lactoferrin.

[0068] Comparative Example 6

[0069] The comparative example provides a nutritional composition powder package beneficial to pet health, which is prepared by mixing 3 parts of pig plasma protein powder and 0.0018 parts of lactoferrin powder. The contents of the active ingredients plasma protein and lactoferrin in the nutritional composition powder package are 2.34 parts of plasma protein and 0.00171 parts of lactoferrin, respectively.

[0070] 3.0018 g of the nutritional composition powder package of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The contents of active ingredients in the finished pet food were: 2.34 wt% of plasma protein and 0.00171 wt% of lactoferrin.

[0071] Comparative Example 7

[0072] The comparative example provides a nutritional composition powder package beneficial to pet health, which is prepared by mixing 0.6 parts of β-glucan raw material, 1.5 parts of porcine plasma protein powder and 0.0008 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.33 parts of β-glucan, 1.17 parts of plasma protein and 0.00076 parts of lactoferrin.

[0073] 2.1008 g of the nutritional composition powder package of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.33 wt% of β-glucan, 1.17 wt% of plasma protein, and 0.00076 wt% of lactoferrin.

[0074] Comparative Example 8

[0075] The comparative example provides a nutritional composition powder package beneficial to pet health, which is prepared by mixing 1.2 parts of β-glucan raw material, 1.5 parts of porcine plasma protein powder and 0.05 parts of lactoferrin powder. In the nutritional composition powder package, the contents of active ingredients β-glucan, plasma protein and lactoferrin are respectively: 0.66 parts of β-glucan, 1.17 parts of plasma protein and 0.0475 parts of lactoferrin.

[0076] 2.75 g of the nutritional composition powder package of this comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of active ingredients in the finished pet food was: 0.66 wt% of β-glucan, 1.17 wt% of plasma protein, and 0.0475 wt% of lactoferrin.

[0077] Trials and tests

[0078] 1. Experimental Methods

[0079] Healthy adult cats (male-female ratio 1:1) of similar age and weight were selected and randomly divided into groups, with 6 cats in each group, and fed in single cages with corresponding pet food. All groups of test cats were provided with a diet that met the AAFCO cat food nutrient composition table, and the feed intake was determined according to the nutritional needs of the test cats during the adult maintenance period. They were free to eat and drink water. The adaptation period was 1 week. Except for the normal control group, cats in other groups were required to undergo intestinal immune modeling (modeling period of 1 week) and a recovery period of 3 weeks. Feces, blood or hair were collected at specific time points for the following scores or tests.

[0080] Intestinal immunity modeling test: Use enteric pathogens such as Escherichia coli for gavage / feeding to establish an intestinal immunity model. Use Escherichia coli for gavage treatment, and feed 5*10 per kg of cat body weight. 10 CFU / mL of bacteria once in the morning and evening. Feed the test cats with pathogenic bacteria for 7 days to ensure that all test cats have diarrhea for 3-4 days, which means the model is successful. After the modeling period, at the beginning of the recovery period, continue to feed the control diet, score the cats' feces daily, and record growth data weekly. Collect blood samples and fecal samples.

[0081] 2. Experimental dose

[0082] Normal control group: Suligao Let's Stay In cat food containing chicken, apples and lentils;

[0083] Model control group: Let's Stay In cat food containing chicken, apples and lentils;

[0084] Experimental group: Let's Stay In cat food containing chicken, apple & lentil formula + powder pack of the composition of the embodiment / comparative example.

[0085] 3. Experimental content

[0086] 3.1 Stool quality

[0087] Score the stool according to Table 2 and take the average value (the closer the score is to 3, the better).

[0088] Table 2

[0089]

[0090]

[0091] The diarrhea rate was calculated by the number of diarrhea episodes during the test period: diarrhea rate (%) = 100 × the number of diarrhea episodes during the test period / the total number of fecal score statistics for cats during the test period.

[0092] 3.2 Nutrient Apparent Digestibility

[0093] In the sixth week, all feces were collected for 5 consecutive days, weighed and stored in a -20℃ freezer for analysis. The contents of crude protein (GB / T 6432-2018), dry matter (GB / T 6435-2014), crude fat (GB / T 6433-2006), crude fiber (GB / T 6434-2006), crude ash (GB / T 6438-2007), calcium (GB / T 6436-2018) and phosphorus (GB / T6437-2018) in feed and feces samples were determined. The gross energy of feed and feces samples was determined using an oxygen bomb calorimeter (ISO 9831:1998). The apparent digestibility of nutrients was calculated as follows:

[0094] Apparent digestibility of a nutrient (%) = (nutrient content in the feed - nutrient content in feces) / (nutrient content in the feed) × 100.

[0095] 3.3 Preparation of blood samples and determination of biochemical indicators

[0096] At the end of the experiment, blood was collected from the forelimb vein of the cats in the experimental group and the control group. A portion of the blood was analyzed for routine blood analysis, and the remaining serum was separated for blood biochemical testing. Blood biochemical test indicators included albumin (ALB), total protein (TP), total bilirubin (TB), inorganic phosphorus (PHOS), calcium (Ca), globulin (GLOB), alanine aminotransferase (ALT) and aspartate aminotransferase (AST).

[0097] Among the above-mentioned blood biochemical indicators, total protein is better between 54.00 and 89.00, albumin is better between 22.00 and 45.00, and globulin is better between 15.00 and 57.00; alanine aminotransferase is better between 8.2 and 100, while higher levels will cause liver damage; aspartate aminotransferase is better between 9.2 and 39.5, while higher levels will cause liver damage.

[0098] 3.4 Hair Health

[0099] On the 42nd day of the experiment, the hair on the back of each cat was collected and the hair score (HS) was performed using the method described by Rees et al. under the same lighting conditions and on the same observation platform. The scoring criteria are shown in Table 3 and Figure 1 .

[0100] Table 3

[0101]

[0102]

[0103] Among them, 1≤HS≤2 is dry; 2<HS<4 is ideal; 4≤HS≤5 is greasy.

[0104] 3.5 Metabolite Detection

[0105] Serum and feces samples were collected from animals to measure the concentrations of metabolites in the feces, including acetic acid, propionic acid, butyric acid, valeric acid, isovaleric acid, and isobutyric acid.

[0106] 3.6 Detection of inflammatory factors and immune factors

[0107] Enzyme-linked immunosorbent assay (ELISA) was used to detect immune indicators such as tumor necrosis factor-α (TNF-α), gamma interferon (IFN-γ), interleukin IL-1β, IL-6, IL-8, IL-10, immunoglobulins IgA, IgG, and IgM in serum, and immune indicators such as immunoglobulins IgA, IgG, and IgM in feces.

[0108] 3.7 Intestinal health index detection

[0109] After the adaptation period, intestinal immune modeling was performed: cats in the experimental group and the model control group were gavaged with bacterial culture fluid (bacteria: enteric pathogenic Escherichia coli), and the individual infection and recovery conditions during the gavage period, fecal scores, and metabolites were observed and recorded.

[0110] 3.8 Data processing and analysis

[0111] Independent sample t-test was used in SPSS26.0 software, and the results were expressed as "mean ± standard error". Letters represent significant differences, and different letters indicate significant differences between groups. Single letters indicate strong significant differences, i.e., p < 0.01; double letters indicate weak significant differences, i.e., p < 0.05.

[0112] Test Example 1: Effects of nutritional composition ingredients on the health of pet cats

[0113] The effects of the ingredients of the nutritional composition on the health of pet cats are shown in Tables 4-5.

[0114] Table 4

[0115]

[0116]

[0117]

[0118] Table 5

[0119]

[0120]

[0121] From Table 4-5 and Figure 2-8 The results show that compared with the normal control group, the pet cats in the model control group showed deterioration in multiple indicators such as digestibility, feces quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health. This indicates that after intestinal immune modeling, the health status of pet cats has declined significantly.

[0122] See Table 4 and Figure 2-8 In Comparative Examples 1 to 3, when β-glucan, plasma protein or lactoferrin is used alone in the nutritional composition, the health of the pet cats can be improved to a certain extent. Compared with the model control group, the pet cats in Comparative Examples 1 to 3 have improved digestibility index, fecal quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health, but are still inferior to the pet cats in the normal control group in most indicators.

[0123] In Comparative Examples 4 to 6, when two of β-glucan, plasma protein and lactoferrin are used in the nutritional composition, the health of the pet cat can be improved to a certain extent, and the effect is better than that of the nutritional composition of Comparative Examples 1 to 3. Figure 2-8It can be seen that the pet cats in Comparative Examples 4 to 6 have a more significant improvement in digestibility index, fecal quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health compared with the model control group, and are also better than the pet cats in Comparative Examples 1 to 3, but are still inferior to the pet cats in the normal control group in most indicators. This shows that compared with using one alone, the combination of β-glucan, plasma protein and lactoferrin can better improve the health of pet cats.

[0124] In Example 1, when the nutritional composition contains β-glucan, plasma protein and lactoferrin at the same time, the results show that the health of the pet cat can be significantly improved, and the effect is better than that of the nutritional compositions of Comparative Examples 1 to 6. Figure 2-8 It can be seen that the pet cats in Example 1 have a more significant improvement in digestibility index, fecal quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health compared with the model control group, and also have a greater improvement compared with comparative examples 1 to 6, and are also better than the pet cats in the normal control group in most indicators. This shows that when the nutritional composition contains β-glucan, plasma protein and lactoferrin at the same time, the three active components can play a synergistic role, which can better improve the intestinal health of pets, enhance immunity, and improve hair health.

[0125] Experimental Example 2: Effect of the dosage of the nutritional composition on the health of pet cats

[0126] The effects of the dosage of the nutritional composition on the health of pet cats are shown in Tables 6-7.

[0127] Table 6

[0128]

[0129]

[0130] Table 7

[0131]

[0132]

[0133] Please refer to Table 6-7 and Figure 9-15The nutritional compositions of Examples 1 to 4 simultaneously contain three active ingredients, β-glucan, plasma protein and lactoferrin, and their ratios are within the range of (0.055-0.275): (1.56-4.68): (0.00095-0.0285). From the results, the pet cats of Examples 1 to 4 have significantly improved digestibility index, fecal quality, fecal immune factors, fecal metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health compared with the model control group, and are comparable to or better than the normal control group.

[0134] See Table 6 and Figure 9-15 The nutritional compositions of Comparative Examples 7 to 8 simultaneously contain three active ingredients: β-glucan, plasma protein and lactoferrin, but their ratios are not within the range of (0.055-0.275): (1.56-4.68): (0.00095-0.0285). From the results, the pet cats of Comparative Examples 7 and 8 have a certain improvement in digestibility index, feces quality, feces immune factors, feces metabolites, blood immune factors and inflammatory factors, serum biochemistry and hair health compared with the model control group, but are still not as good as the normal control group, and there is still a certain gap compared with the pet cats of Example 1. This shows that when the three active ingredients of β-glucan, plasma protein and lactoferrin in the nutritional composition are compounded in the ratio of (0.055-0.275):(1.56-4.68):(0.00095-0.0285), their synergistic effect can be better exerted, thereby comprehensively improving the intestinal health of pets, enhancing immunity, and improving hair health.

[0135] In summary, the composition provided by the present invention can comprehensively improve the health of pets. Specifically, it can improve the intestinal health of pets, reduce the diarrhea rate, and also improve the immunity of pets and improve the hair health of pets.

[0136] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A nutritional composition beneficial to pet health, characterized in that: The nutritional composition comprises β-glucan, plasma protein and lactoferrin; wherein the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.275):(1.56-4.68):(0.00095-0.0285).

2. A nutritional composition beneficial to pet health according to claim 1, characterized in that: In the nutritional composition, the mass ratio of β-glucan, plasma protein and lactoferrin is (0.055-0.165):(1.56-3.12):(0.00095-0.019).

3. A nutritional composition beneficial to pet health according to claim 1, characterized in that: In the nutritional composition, the mass ratio of β-glucan, plasma protein and lactoferrin is 0.1485:2.34:0.00171.

4. Use of the composition according to any one of claims 1 to 3 in improving the intestinal health of pets.

5. Use of the composition according to any one of claims 1 to 3 in improving the immunity of pets.

6. Use of the composition according to any one of claims 1 to 3 in improving the health of pet hair.

7. Use of the composition according to any one of claims 1 to 3 in preparing pet food.

8. A pet food, characterized in that: The pet food comprises the nutritional composition according to any one of claims 1 to 3.

9. The pet food according to claim 8, characterized in that: The nutritional composition is added to the pet food in an amount of 0.1 to 10 wt %.

10. The pet food according to claim 8, characterized in that: In the pet food, the content of β-glucan is 0.055-0.275wt%, the content of plasma protein is 1.56-4.68wt%, and the content of lactoferrin is 0.00095-0.0285wt%.

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