A composite solid beverage for alleviating and / or improving osteoporosis and its preparation method and application

Abstract

The present invention relates to a composite solid beverage for alleviating and / or improving osteoporosis and a preparation method and application thereof, belonging to the technical field of health food. The composite solid beverage of the present invention comprises the following components: cucumber seed powder, walnut powder, kudzu root powder, elderberry powder, composite grain powder, resistant dextrin, bovine bone collagen peptide, type II collagen peptide, milk calcium, composite vitamins, composite minerals, sweeteners, edible flavors, xanthan gum, and sodium carboxymethyl cellulose; wherein the composite vitamins include VA, VB1, VB6, VB 12 , VC, VD, VE, folic acid and pantothenic acid; and composite minerals include iron and magnesium. The composite solid beverage of the present invention can inhibit bone absorption and promote the formation of normal bone mass, and has the characteristics of good absorption effect, significant relief and / or improvement effect and no side effects.

Description

Technical Field

[0001] The present invention relates to the technical field of health food, and in particular to a composite solid beverage for alleviating and / or improving osteoporosis, and a preparation method and application thereof. Background Art

[0002] Osteoporosis is a common chronic systemic bone metabolic disease, whose pathological characteristics are decreased mineralized bone mass, destruction of bone tissue microstructure, decreased bone density, impaired bone strength and susceptibility to fractures. Osteoporosis can occur at any age, especially in postmenopausal women, making the prevalence of osteoporosis in women much higher than that in men, and the risk of bone fragility is also significantly higher than that in men. Osteoporotic fractures caused by the deterioration of osteoporosis have a significant complication rate and mortality risk, which imposes a serious economic burden on patients, families and society.

[0003] At present, the clinical treatment drugs for osteoporosis are mainly chemical drugs, such as bisphosphonates, estrogen, calcitonin, etc. These drugs have certain clinical treatment effects; common osteoporosis prevention products are mainly calcium supplements, such as liquid calcium, chewable calcium, solid beverages, etc. However, long-term use of chemical drugs is very likely to cause adverse reactions such as gastrointestinal damage; calcium supplements are expensive and the effect is not obvious, and some products will have the risk of stone disease after long-term consumption; and one of the raw materials of solid beverages is calcium carbonate, which belongs to the first generation of inorganic salt calcium supplements, with many defects such as large gastrointestinal irritation, low calcium absorption rate, low bone precipitation rate, and long-term consumption will cause stones or vascular sclerosis.

[0004] There is no health food in the prior art that can effectively alleviate and / or improve osteoporosis and has few adverse reactions. Summary of the invention

[0005] The purpose of the present invention is to provide a composite solid beverage for alleviating and / or improving osteoporosis and a preparation method and application thereof, so as to solve the problems in the prior art of high product cost, many side effects and unclear effect on alleviating and / or improving osteoporosis.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a composite solid beverage for alleviating and / or improving osteoporosis, comprising the following components in parts by weight: 50-70 parts of cucumber seed powder, 2-8 parts of walnut powder, 0.5-3 parts of kudzu root powder, 0.5-4 parts of elderberry powder, 10-20 parts of composite grain powder, 1-5 parts of resistant dextrin, 2-10 parts of bovine collagen peptide, 1-4 parts of type II collagen peptide, 0.5-3 parts of milk calcium, 0.1-0.5 parts of composite vitamins, 0.3-0.9 parts of composite minerals, 0.015-0.03 parts of sweeteners, 0.4-0.8 parts of edible flavors, 0.1-0.6 parts of xanthan gum, and 0.1-0.5 parts of sodium carboxymethyl cellulose;

[0008] The vitamin complex comprises the following components in mass ratio: VA:VB 1 :VB 6 :VB 12 :VC:VD:VE:Folic acid:Pantothenic acid=896~1344:1383.9~2076:1599.85~2399.95:4.0~5.999:168000~252000:1.59985~2.39995:10801~16201.5:80.15~120.05:5600~8400;

[0009] The composite mineral comprises components in the following mass ratio: iron:magnesium=7.998-12:103.98-156.

[0010] Preferably, the sweetener comprises one or more of sucralose, maltitol and steviol glycoside.

[0011] Preferably, the edible flavor includes but is not limited to malt flavor.

[0012] Preferably, the particle sizes of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder, and composite grain powder are independently 20 to 40 meshes;

[0013] The water content of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder and composite grain powder is independently less than 7%.

[0014] The present invention also provides a method for preparing the composite solid beverage, comprising the following steps:

[0015] (1) premixing walnut powder, kudzu root powder, elderberry powder, resistant dextrin, bovine bone collagen peptide, type II collagen peptide, milk calcium, complex vitamins, complex minerals, sweeteners, edible flavors, xanthan gum, and sodium carboxymethyl cellulose to obtain a premix;

[0016] (2) The premix, cucumber seed powder and composite grain powder are mixed to obtain a composite solid beverage.

[0017] Preferably, the premixing speed in step (1) is 10 to 30 rpm, and the premixing time is 10 to 20 min.

[0018] Preferably, the mixing speed in step (2) is 10 to 30 rpm, and the mixing time is 30 to 60 min.

[0019] The present invention also provides the use of the composite solid beverage in preparing food for alleviating and / or improving osteoporosis.

[0020] The present invention also provides the use of the composite solid beverage prepared by the preparation method in preparing food for alleviating and / or improving osteoporosis.

[0021] The present invention has the following technical effects and advantages:

[0022] (1) The composite solid beverage of the present invention comprises a plurality of components that play a positive role in improving and / or alleviating osteoporosis, wherein cucumber seed powder is rich in calcium, with a content of up to 898.6 mg / 100 g, being a veritable "calcium source", and a phosphorus content of 0.72 g / 100 g, which can effectively promote the body's absorption of calcium, thereby having a restorative, regulating and health-care effect on osteoporosis, arthritis, leg cramps and other diseases; walnut powder has extremely high nutritional value and is known as the "king of antioxidants", and has a promoting effect on protecting cardiovascular system and treating coronary heart disease; puerarin contained in kudzu root powder can dilate coronary arteries and cerebral arteries, and further comprises a plurality of trace elements such as calcium, zinc, phosphorus, selenium and amino acids, having a certain The invention combines cucumber seed powder with kudzu root powder, walnut powder and elderberry powder, which are both medicinal and edible, for the first time to form a nutritional combination, which has all-round health care functions such as enhancing immunity and improving intestinal health. The composite grain powder is rich in folic acid and γ-aminobutyric acid. Folic acid can promote calcium absorption, thereby preventing osteoporosis, and γ-aminobutyric acid has the function of balancing bone metabolism and improving bone performance. Bovine bone collagen peptide and type II collagen peptide can promote bone and joint health. Milk calcium has the characteristics of high absorption rate. The composite minerals composed of iron and magnesium can promote calcium absorption and maintain bone health. The composite solid beverage of the invention can inhibit bone absorption by reducing serum β-CTx content and the number of osteoclasts, thereby promoting the formation of normal bone. It has the advantages of good absorption effect, significant improvement and / or relief effect, and no side effects.

[0023] (2) In the process of preparing the composite solid beverage, the present invention adopts a method of premixing a small amount of raw material components and then mixing them with other raw material components, which can achieve a better mixing effect. The method has the advantages of being simple, easy to operate and implement, and suitable for large-scale production in enterprises. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The figures are the results of HE staining of paraffin sections of femoral tissues of mice in each group, among which A is the cross section of femoral tissues of mice in the blank group, B is the cross section of femoral tissues of mice in the positive group, C is the cross section of femoral tissues of mice in the model group, D is the cross section of femoral tissues of mice in the low-dose group, E is the cross section of femoral tissues of mice in the medium-dose group, and F is the cross section of femoral tissues of mice in the high-dose group. DETAILED DESCRIPTION

[0025] The present invention provides a composite solid beverage for alleviating and / or improving osteoporosis, comprising the following components in parts by weight: 50 to 70 parts, preferably 55 to 65 parts, and more preferably 62.5 parts, of cucumber seed powder; 2 to 8 parts, preferably 4 to 6 parts, and more preferably 5 parts, of walnut powder; 0.5 to 3 parts, preferably 0.8 to 1.5 parts, and more preferably 1 part, of kudzu root powder; 0.5 to 4 parts, preferably 1 to 3 parts, and more preferably 2 parts, of elderberry powder; 10 to 20 parts, preferably 13 to 17 parts, and more preferably 15.43 parts, of composite grain powder; 1 to 5 parts, preferably 1.5 to 3 parts, and more preferably 2.5 parts, of resistant dextrin; 2 to 10 parts, preferably 4 to 7 parts, and more preferably 5 parts, of bovine collagen peptide; and 1 to 4 parts, preferably 2 to 3 parts, of type II collagen peptide. , more preferably 2.5 parts; milk calcium 0.5-3 parts, preferably 1.5-2.5 parts, more preferably 2 parts; complex vitamins 0.1-0.5 parts, preferably 0.2-0.4 parts, more preferably 0.35 parts; complex minerals 0.3-0.9 parts, preferably 0.5-0.7 parts, more preferably 0.6 parts; sweeteners 0.015-0.03 parts, preferably 0.018-0.025 parts, more preferably 0.02 parts; edible flavors 0.4-0.8 parts, preferably 0.5-0.7 parts, more preferably 0.6 parts; xanthan gum 0.1-0.6 parts, preferably 0.2-0.4 parts, more preferably 0.3 parts; sodium carboxymethyl cellulose 0.1-0.5 parts, preferably 0.15-0.25 parts, more preferably 0.2 parts;

[0026] The vitamin complex comprises the following components in mass ratio: VA:VB 1 :VB 6 :VB 12:VC:VD:VE:Folic acid:Pantothenic acid=896~1344:1383.9~2076:1599.85~2399.95:4.0~5.999:168000~252000:1.59985~2.39995:10801~16201.5:80.15~120.05:5600~8400, preferably 1000~1 200:1500~1800:1800~2100:4.5~5.5:180000~230000:1.7~2.1:12000~14000:95~105:6000~8000, more preferably 1120:1730:1999.9:5:210000:1.9999:13501:100.1:7000;

[0027] The composite mineral comprises components in the following mass ratio: iron:magnesium=7.998-12:103.98-156, preferably 9-11:110-140, and more preferably 9.999:129.99.

[0028] In the present invention, the preparation method of the cucumber seed powder comprises the following steps: mixing cucumber seeds and water for soaking, adding cellulase for enzymolysis, crushing, filtering, taking the filtrate to obtain cucumber seed enzymolysis solution; spray drying the cucumber seed enzymolysis solution to obtain cucumber seed powder;

[0029] The mass volume ratio of the cucumber seeds and water is 1g:5-15mL, preferably 1g:10mL;

[0030] The soaking time is 1.5 to 3 hours, preferably 2 hours;

[0031] The cellulase has an enzymatic activity of 80,000 to 120,000 U / g, preferably 100,000 U / g;

[0032] The mass ratio of the cucumber seeds to the cellulase is 100:0.5-2, preferably 100:1;

[0033] The cellulase was purchased from Xi'an Qiannuo Bioengineering Co., Ltd.

[0034] The enzymatic hydrolysis temperature is 45-65°C, preferably 60°C; the enzymatic hydrolysis time is 20-60min, preferably 30min;

[0035] The inlet air temperature of the spray drying is 130-190°C, preferably 160°C; the outlet air temperature of the spray drying is 70-90°C, preferably 80°C.

[0036] In the present invention, the composite grain powder is enzymatically hydrolyzed germinated grain powder I, comprising brown rice, soybeans, oats, germinated brown rice, and beer malt, purchased from Tianjin Fuhaitai Technology Co., Ltd.

[0037] In the present invention, the sweetener includes one or more of sucralose, maltitol and steviol glycoside.

[0038] In the present invention, the edible flavor includes but is not limited to malt flavor.

[0039] In the present invention, the particle sizes of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder, and composite grain powder are independently 20 to 40 meshes, preferably 30 meshes;

[0040] The water content of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder and composite grain powder is independently less than 7%, preferably 5%.

[0041] The present invention also provides a method for preparing the composite solid beverage, comprising the following steps:

[0042] (1) premixing walnut powder, kudzu root powder, elderberry powder, resistant dextrin, bovine bone collagen peptide, type II collagen peptide, milk calcium, complex vitamins, complex minerals, sweeteners, edible flavors, xanthan gum, and sodium carboxymethyl cellulose to obtain a premix;

[0043] (2) The premix, cucumber seed powder and composite grain powder are mixed to obtain a composite solid beverage.

[0044] In the present invention, the premixing speed in step (1) is 10 to 30 rpm, preferably 25 rpm; the premixing time is 10 to 20 min, preferably 15 min.

[0045] In the present invention, the mixing speed in step (2) is 10 to 30 rpm, preferably 25 rpm; the mixing time is 30 to 60 min, preferably 40 min.

[0046] In the present invention, the step (2) further comprises bagging, nitrogen filling, and heat sealing after the mixing;

[0047] The bagging mass is 8-10 g / bag, preferably 9 g / bag;

[0048] The residual oxygen content of the nitrogen filling is ≤8%, preferably 5%;

[0049] The outer transverse sealing temperature of the heat seal is 100-140°C, preferably 120°C; the inner transverse sealing temperature of the heat seal is 140-160°C, preferably 150°C; the longitudinal sealing temperature of the heat seal is 150-180°C, preferably 160°C.

[0050] The present invention also provides the use of the composite solid beverage in preparing food for alleviating and / or improving osteoporosis.

[0051] The present invention also provides the use of the composite solid beverage prepared by the preparation method in preparing food for alleviating and / or improving osteoporosis.

[0052] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0053] In the present invention, the raw material components of the enzymatically hydrolyzed germinated grain powder I include brown rice, soybeans, oats, germinated brown rice, and beer malt, which are purchased from Tianjin Fuhaitai Technology Co., Ltd.;

[0054] In the present invention, cellulase was purchased from Xi'an Qiannuo Bioengineering Co., Ltd.

[0055] In the present invention, alendronate sodium (product batch number: A2293119D) was purchased from Chengdu Desit Biotechnology Co., Ltd., and mouse type I collagen amino-terminal extended peptide (P1NP) ELISA kit (product batch number: YMS9389-A) and mouse β-collagen degradation product (β-CTx) ELISA kit (product batch number: YMS30333-A) were purchased from Chengdu Yuannuo Tiancheng Technology Co., Ltd.;

[0056] In the present invention, the mixer was purchased from Changzhou Junnuo Drying Equipment Co., Ltd., the multi-row back-sealed automatic powder packaging machine was purchased from Beijing Xinzhida Packaging Machinery Co., Ltd., and the NIKON ECLIPSE CI microscope was purchased from Nikon Corporation of Japan;

[0057] In the present invention, the test mice were 4-week-old Kunming mice, weighing 18-22 g, purchased from Chengdu Dashuo Experimental Animal Co., Ltd.

[0058] Example 1: Preparation of a composite solid beverage

[0059] (1) Take 1120 mg VA (in terms of retinol equivalent RE), 1730 mg VB 1 、1999.9mgVB 6 、5mg VB 12 , 210000 mg VC, 1.9999 mg VD, 13501 mg VE (calculated as α-tocopherol equivalent α-TE), 100.1 mg folic acid and 7000 mg pantothenic acid are mixed evenly to obtain a vitamin complex;

[0060] (2) 9.999 g of iron and 129.99 g of magnesium were mixed evenly to obtain a composite mineral;

[0061] (3) 100 g of cucumber seeds and 1000 mL of water were mixed, and after soaking for 2 h, 1 g of cellulase with an enzyme activity of 100,000 U / g was added, and enzymolysis was performed at 60° C. for 30 min, and then crushed and filtered to obtain a cucumber seed enzymatic solution; the cucumber seed enzymatic solution was spray-dried under the conditions of an inlet air temperature of 160° C. and an outlet air temperature of 80° C. to obtain cucumber seed powder;

[0062] (4) Passing walnut powder, kudzu root powder and elderberry powder through a 30-mesh sieve, taking 5 g of walnut powder, 1 g of kudzu root powder, 2 g of elderberry powder, 2.5 g of resistant dextrin, 5 g of bovine collagen peptide, 2.5 g of type II collagen peptide, 2 g of milk calcium, 0.35 g of multivitamins, 0.6 g of multiminerals, 0.02 g of sucralose, 0.6 g of wheat flavor powder, 0.3 g of xanthan gum and 0.2 g of sodium carboxymethyl cellulose, pouring the sieved walnut powder into a mixer, and premixing at 25 rpm for 15 min to obtain a premix;

[0063] (5) Passing the cucumber seed powder and the enzymatically hydrolyzed germinated grain powder I through a 30-mesh sieve, taking 62.5 g of the sieved cucumber seed powder, 15.43 g of the enzymatically hydrolyzed germinated grain powder I and the premix prepared in step (3) into a mixer, and mixing at 25 rpm for 40 min to obtain a composite solid beverage;

[0064] Among them, the water content of cucumber seed powder is 5%, the water content of walnut powder is 5%, the water content of kudzu root powder is 5%, the water content of elderberry powder is 5%, and the water content of enzymatically hydrolyzed sprouted grain powder I is 5%;

[0065] (6) After the composite solid beverage is packed into bags according to the specification of 8 g / bag, nitrogen is filled into the bags until the residual oxygen content is 5%, and heat-sealed using a multi-row back-sealing automatic powder packaging machine at an outer transverse sealing temperature of 120°C, an inner transverse sealing temperature of 150°C, and a longitudinal sealing temperature of 160°C.

[0066] Example 2: Preparation of composite solid beverage

[0067] (1) Take 896 mg VA (in terms of retinol equivalent RE), 2076 mg VB 1 、2399.95mgVB 6 、4mg VB 12 , 168000 mg VC, 2.39995 mg VD, 16201.5 mg VE (calculated as α-tocopherol equivalent α-TE), 80.15 mg folic acid and 8400 mg pantothenic acid are mixed evenly to obtain a vitamin complex;

[0068] (2) taking 7.998 g of iron and 156 g of magnesium and mixing them evenly to obtain a composite mineral;

[0069] (3) 100 g of cucumber seeds and 1500 mL of water were mixed, and after soaking for 1.5 h, 0.5 g of cellulase with an enzyme activity of 120,000 U / g was added, and enzymolysis was performed at 65° C. for 20 min, and then crushed and filtered to obtain a cucumber seed hydrolyzate; the cucumber seed hydrolyzate was spray-dried under the conditions of an inlet air temperature of 130° C. and an outlet air temperature of 70° C. to obtain cucumber seed powder;

[0070] (4) 8 g of walnut powder, kudzu root powder, and elderberry powder are sieved through a 20-mesh sieve, and 8 g of walnut powder, 3 g of kudzu root powder, 1 g of elderberry powder, 1.5 g of resistant dextrin, 4 g of bovine collagen peptide, 3 g of type II collagen peptide, 1.5 g of milk calcium, 0.2 g of multivitamins, 0.9 g of multiminerals, 0.015 g of stevioside, 0.8 g of wheat flavor powder, 0.4 g of xanthan gum, and 0.25 g of sodium carboxymethyl cellulose are poured into a mixer and premixed at 30 rpm for 10 min to obtain a premix;

[0071] (5) Passing the cucumber seed powder and the enzymatically hydrolyzed germinated grain powder I through a 20-mesh sieve, taking 65 g of the sieved cucumber seed powder, 17 g of the enzymatically hydrolyzed germinated grain powder I and the premix prepared in step (3) into a mixer, and mixing at 30 rpm for 30 min to obtain a composite solid beverage;

[0072] Among them, the water content of cucumber seed powder is 5%, the water content of walnut powder is 5%, the water content of kudzu root powder is 5%, the water content of elderberry powder is 5%, and the water content of enzymatically hydrolyzed sprouted grain powder I is 5%;

[0073] (6) After the composite solid beverage is packed into bags according to the specification of 9 g / bag, nitrogen is filled into the bags until the residual oxygen content is 5%, and heat-sealed using a multi-row back-sealing automatic powder packaging machine at an outer transverse sealing temperature of 100°C, an inner transverse sealing temperature of 140°C, and a longitudinal sealing temperature of 180°C.

[0074] Example 3: Preparation of composite solid beverage

[0075] (1) Take 1200 mg VA (in terms of retinol equivalent RE), 1383.9 mg VB 1 、1599.85mg VB 6 、5.5mgVB 12 , 180000 mg VC, 2.1 mg VD, 10801 mg VE (calculated as α-tocopherol equivalent α-TE), 120.05 mg folic acid and 5600 mg pantothenic acid are mixed evenly to obtain a vitamin complex;

[0076] (2) 11 g of iron and 103.98 g of magnesium were mixed evenly to obtain a composite mineral;

[0077] (3) 100 g of cucumber seeds and 500 mL of water were mixed, and after soaking for 3 h, 2 g of cellulase with an enzyme activity of 80,000 U / g was added, and enzymolysis was performed at 45° C. for 60 min, and then crushed and filtered to obtain a cucumber seed enzymatic solution; the cucumber seed enzymatic solution was spray-dried under the conditions of an inlet air temperature of 190° C. and an outlet air temperature of 90° C. to obtain cucumber seed powder;

[0078] (4) 40-mesh sieve is used to sieve walnut powder, kudzu root powder and elderberry powder, and 4 g walnut powder, 1.5 g kudzu root powder, 4 g elderberry powder, 5 g resistant dextrin, 10 g bovine collagen peptide, 4 g type II collagen peptide, 0.5 g milk calcium, 0.5 g multivitamins, 0.7 g multiminerals, 0.03 g maltitol, 0.4 g malt flavor powder, 0.1 g xanthan gum and 0.15 g sodium carboxymethyl cellulose are poured into a mixer and premixed at 10 rpm for 20 min to obtain a premix;

[0079] (5) Passing the cucumber seed powder and the enzymatically hydrolyzed germinated grain powder I through a 40-mesh sieve, taking 70 g of the sieved cucumber seed powder, 13 g of the enzymatically hydrolyzed germinated grain powder I and the premix prepared in step (3) into a mixer, and mixing at 10 rpm for 60 min to obtain a composite solid beverage;

[0080] Among them, the water content of cucumber seed powder is 5%, the water content of walnut powder is 5%, the water content of kudzu root powder is 5%, the water content of elderberry powder is 5%, and the water content of enzymatically hydrolyzed sprouted grain powder I is 5%;

[0081] (6) After the composite solid beverage is packed into bags according to the specification of 10 g / bag, nitrogen is filled into the bags until the residual oxygen content is 5%, and heat-sealed by a multi-row back-sealing automatic powder packaging machine at an outer transverse sealing temperature of 140°C, an inner transverse sealing temperature of 160°C, and a longitudinal sealing temperature of 150°C.

[0082] Experimental Example 1: Mouse Modeling and Treatment

[0083] (1) Modeling: 66 4-week-old Kunming mice were divided into 6 groups, namely, model group, low-dose group, medium-dose group, high-dose group, positive group and blank group, with 11 mice in each group. The mice in the model group, low-dose group, medium-dose group, high-dose group and positive group were injected with 2.5 mg / kg of mouse body weight of dexamethasone by subcutaneous injection for 4 consecutive weeks to obtain osteoporosis mouse models. The mice in the blank group were injected with 0.01 mL / g of mouse body weight of normal saline by subcutaneous injection.

[0084] (2) Administration: The mice in the low-dose group were administered 7 g / kg of the composite solid beverage prepared in Example 1, the mice in the medium-dose group were administered 14 g / kg of the composite solid beverage prepared in Example 1, the mice in the high-dose group were administered 21 g / kg of the composite solid beverage prepared in Example 1, and the mice in the positive group were administered 1.95 mg / kg of the alendronate sodium, for a total of 21 days; the mice in the model group and the blank group were administered corresponding volumes of water;

[0085] (3) Body weight measurement: The body weights of mice in each group were recorded on days 1, 3, 5, 7, 14, and 21 after administration. The results are shown in Table 1.

[0086] Table 1 Body weight test results of mice in each group

[0087] Group 1d No. 3d No. 5d No. 7d 14d 21d Model group (g) 29.5±1.5* 32.5±1.3* 33.8±2.0* 35.6±2.6* 39.1±2.0* 40.9±2.7* Low dose group (g) 30.5±2.7* 35.3±2.9* 35.5±2.4* <![CDATA[38.6±1.9* # ]]> <![CDATA[42.5±2.7* # ]]> <![CDATA[44.0±1.9 # ]]> Medium dose group (g) 29.9±1.7* 32.5±1.7* 35.4±1.9* 36.8±1.8* 41.4±3.1* 43.8±2.3 High dose group (g) 29.3±1.2* 34.4±1.3* 35.9±1.5* 36.4±1.2* <![CDATA[41.8±1.3* # ]]> <![CDATA[42.0±1.5 # ]]> Positive group (g) 31.1±2.4* 36.3±1.7 <![CDATA[38.4±3.1* # ]]> <![CDATA[38.9±2.9* # ]]> <![CDATA[42.5±1.5* # ]]> <![CDATA[43.9±4.5 # ]]> Blank group (g) 37.4±1.5 37.4±1.4 44.0±1.5 44.1±1.9 44.3±2.1 44.5±2.1

[0088] In Table 1, * indicates P < 0.05 compared with the blank group. # Indicates P < 0.05 compared with the model group.

[0089] The results showed that after 21 days of administration to each group of mice, the body weight of the mice in the low-dose group, the medium-dose group and the high-dose group increased significantly compared with the mice in the model group (P < 0.05), indicating that the composite solid beverage of the present invention can effectively supplement the nutrients required for the normal growth and development of osteoporosis model mice, thereby increasing the body weight of osteoporosis model mice.

[0090] Experimental Example 2: Determination of serum index content in mice

[0091] The serum of each group of mice after 21 days of administration in Experimental Example 1 was taken, and the β-CTx content and P1NP content in the serum of each group of mice were determined using a mouse β-collagen degradation product (β-CTx) ELISA kit and a mouse type I collagen amino-terminal extended peptide (P1NP) ELISA kit, respectively. The results are shown in Table 2.

[0092] Table 2 Results of mouse serum index content detection

[0093] Group β-CTx (pg / mL) P1NP (nmol / L) Model Group 200.67±24.41** 9.2±0.2** Low dose group <![CDATA[162.77±18.85 # ]]> 9.6±0.6 Medium dose group <![CDATA[162.03±14.49 # ]]> <![CDATA[9.9±0.3 # ]]> High dose group <![CDATA[152.3±26.73 ## ]]> <![CDATA[9.9±0.4 # ]]> Positive group <![CDATA[157.37±12.72 # ]]> <![CDATA[10.2±0.7 # ]]> Blank Group <![CDATA[150.54±27.22 ## ]]> <![CDATA[10.1±0.5 # ]]>

[0094] In Table 2, * indicates P < 0.05 compared with the blank group, and ** indicates P < 0.01 compared with the blank group; # Compared with the model group, P < 0.05. ## Indicates P < 0.01 compared with the model group.

[0095] The results showed that after 21 days of administration to each group of mice, compared with the model group mice, the β-CTx content in the serum of the low-dose group, the medium-dose group and the high-dose group mice was significantly reduced (P < 0.01), and the P1NP content was significantly increased (P < 0.05), indicating that the composite solid beverage of the present invention can reduce the degradation of type I collagen on the bone matrix by osteoclasts by reducing the β-CTx content of osteoporosis model mice, thereby inhibiting bone resorption. And according to the P1PN content detection results, it can be seen that the composite solid beverage of the present invention can promote the synthesis of type I collagen in osteoporosis model mice. Type I collagen and other non-collagen proteins such as osteocalcin and matrix proteins together constitute the matrix framework of the bone, allowing inorganic substances such as calcium and phosphorus to be deposited thereon to form normal bone.

[0096] Experimental Example 3: Mouse Femoral Pathology Detection

[0097] Femoral samples of mice in each group 21 days after administration in Experimental Example 1 were taken, and the degree of femoral tissue pathological damage was observed under a NIKONECLIPSE CI microscope after HE staining of paraffin sections. The number of osteoclasts in the femur of mice was counted at 100×. The results are as follows: Figure 1 And as shown in Table 3.

[0098] Table 3 Results of osteoclast count detection in each group of mice

[0099] Group Number of osteoclasts Model Group 32±4.16** Low dose group <![CDATA[22±3.51 # ]]> Medium dose group <![CDATA[22±7.64 # ]]> High dose group <![CDATA[21±2.0 # ]]> Positive group <![CDATA[23±5.56 ## ]]> Blank Group <![CDATA[24±2.65 ## ]]>

[0100] In Table 3, * indicates P < 0.05 compared with the blank group, and ** indicates P < 0.01 compared with the blank group; # Compared with the model group, P < 0.05. ## Indicates P < 0.01 compared with the model group.

[0101] The results of observation on the degree of pathological damage of the femoral tissue of mice showed that the number of trabeculae in the femoral samples of mice in the model group was slightly reduced, the distance between trabeculae was widened, and fat vacuoles were found in the bone marrow cavity; after 21 days of drug administration to mice in each group, the tissue structure of the femoral samples of mice in the low-dose group, medium-dose group and high-dose group was relatively normal, with abundant trabeculae, which was improved compared with the mice in the model group, and fat vacuoles were found in the bone marrow cavity; there was no significant difference in the width of trabeculae in the femoral samples of mice in each group;

[0102] The results of the test on the number of osteoclasts in mice showed that after injection of dexamethasone, the number of osteoclasts in the model group mice was significantly increased compared with that in the blank group mice; after 21 days of administration to each group of mice, the number of osteoclasts in the low-dose group, medium-dose group and high-dose group mice was significantly decreased compared with that in the model group mice (P<0.05);

[0103] This indicates that the composite solid beverage of the present invention can inhibit bone resorption by reducing the number of osteoclasts in mice, thereby achieving the effect of promoting the formation of normal bone.

[0104] As can be seen from the above embodiments, the present invention provides a composite solid beverage for alleviating and / or improving osteoporosis, and a preparation method and application thereof. The composite solid beverage of the present invention can increase the body weight of osteoporosis model mice, inhibit bone resorption by reducing the β-CTx content in mouse serum and the activity of osteoclasts, thereby achieving the effect of promoting the formation of normal bone, and has the advantages of good absorption effect, significant alleviating and / or improving effect, and no side effects.

[0105] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite solid beverage for alleviating and / or improving osteoporosis, characterized in that: The invention comprises the following components in parts by weight: 50-70 parts of cucumber seed powder, 2-8 parts of walnut powder, 0.5-3 parts of kudzu root powder, 0.5-4 parts of elderberry powder, 10-20 parts of composite grain powder, 1-5 parts of resistant dextrin, 2-10 parts of bovine collagen peptide, 1-4 parts of type II collagen peptide, 0.5-3 parts of milk calcium, 0.1-0.5 parts of complex vitamins, 0.3-0.9 parts of complex minerals, 0.015-0.03 parts of sweeteners, 0.4-0.8 parts of edible flavors, 0.1-0.6 parts of xanthan gum, and 0.1-0.5 parts of sodium carboxymethyl cellulose; The vitamin complex comprises the following components in mass ratio: VA: VB1: VB6: VB 12 :VC:VD:VE:Folic acid:Pantothenic acid=896~1344:1383.9~2076:1599.85~2399.95:4.0~5.999:168000~252000:1.59985~2.39995:10801~16201.5:80.15~120.05:5600~8400; The composite mineral comprises components in the following mass ratio: iron:magnesium=7.998-12:103.98-156.

2. The composite solid beverage according to claim 1, characterized in that: The sweetener includes one or more of sucralose, maltitol and steviol glycoside.

3. The composite solid beverage according to claim 2, characterized in that: The edible flavors include but are not limited to malt flavor powder.

4. The composite solid beverage according to claim 3, characterized in that: The particle sizes of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder, and composite grain powder are independently 20 to 40 meshes; The water content of the cucumber seed powder, walnut powder, kudzu root powder, elderberry powder and composite grain powder is independently less than 7%.

5. The method for preparing a composite solid beverage according to any one of claims 1 to 4, characterized in that: The steps include: (1) premixing walnut powder, kudzu root powder, elderberry powder, resistant dextrin, bovine bone collagen peptide, type II collagen peptide, milk calcium, complex vitamins, complex minerals, sweeteners, edible flavors, xanthan gum, and sodium carboxymethyl cellulose to obtain a premix; (2) The premix, cucumber seed powder and composite grain powder are mixed to obtain a composite solid beverage.

6. The preparation method according to claim 5, characterized in that: The premixing speed in step (1) is 10 to 30 rpm, and the premixing time is 10 to 20 minutes.

7. The preparation method according to claim 6, characterized in that: The mixing speed in step (2) is 10 to 30 rpm, and the mixing time is 30 to 60 min.

8. Use of the composite solid beverage according to any one of claims 1 to 4 in preparing food for alleviating and / or improving osteoporosis.

9. Use of the composite solid beverage prepared by the preparation method according to claims 5 to 7 in preparing food for alleviating and / or improving osteoporosis.

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