The invention relates to a biological plasticized specimen capable of being opened from back to display the visceral positional relationship, and a preparation method thereof, and belongs to the technical field of biological plasticization. The preparation method comprises the following steps: 1, breaking ribs of a preservative-treated human body specimen from the back to expose visceral organs from the back, separating the sacrum and the hip bone at the sacroiliac joint in a manner of complete separation at one side and ligament reservation at the other side, bleaching the human body specimen, soaking the dissected human body specimen in an acetone solution for 60-80 d, putting the dehydrated and degreased human body specimen in a mixed solution of silica gel and a silica gel catalyst ina vacuum pressure cabin, standing for 50-60 d, adjusting the pressure of the vacuum pressure bin from 0.1-0.3 kPa to 0.9-1.2 kPa during standing, shaping and repairing the impregnated human body specimen, putting the human body specimen in a curing box, and curing the human body specimen by using organic peroxide and aliphatic nitrogen compound steam to obtain the biological plasticized specimen.The biological plasticized specimen displays the shape and position relation of visceral organs from the back, so later specimen observation is facilitated.