A composition containing sheep placenta peptide and its preparation process

Abstract

The present invention discloses a composition containing sheep placenta peptide, which comprises 8%-16% sheep placenta peptide, 2%-5% ergothioneine, and 3%-10% first plant extract by mass percentage. The present invention also provides a process for preparing the composition and a cosmetic comprising the composition.

Description

Technical Field

[0001] The invention relates to a composition containing sheep placenta peptide and a preparation process thereof, and also relates to cosmetics containing the sheep placenta peptide composition. Background Art

[0002] According to the Compendium of Materia Medica, "Sheep placenta is the fetus in the ewe's belly. It is sweet and warm in nature. It has the effects of invigorating qi and replenishing deficiency, warming the middle and lower body, and regulating kidney deficiency and emaciation." Sheep placenta contains a variety of ingredients such as amino acids, proteins, enzymes, vitamins and growth factors. It is favored by consumers for its high nutritional value and has been widely used in health products, cosmetics, etc.

[0003] At present, most products on the market use sheep placenta in the form of added sheep placenta extract. However, the molecular weight of sheep placenta extract is greater than 10,000D, which is a foreign protein to the human body, difficult to absorb and has a certain degree of allergenicity.

[0004] Compared with sheep placenta, sheep placenta peptide has a smaller molecular weight, stronger activity, and is relatively easier to be absorbed by the human body. Sheep placenta peptide is rich in a variety of small molecule peptide active ingredients, such as antioxidant peptides, immune active peptides, cytokines, growth factors, enzymes that regulate metabolism, hormones, etc. However, due to its complex composition, when it is used in cosmetics, health care products and other products, it may interact with other compounds in the product, resulting in its various active ingredients not being fully absorbed.

[0005] Therefore, there is a need in the art to provide a composition containing sheep placental peptides, which can promote more complete absorption of the active ingredients in the sheep placental peptides by the human body.

[0006] The present invention also has the advantage of providing a preparation process of a composition containing sheep placenta peptide, and the process combines the characteristics of each component to prepare a more uniform and stable composition. Summary of the invention

[0007] Brief description of the invention

[0008] In one aspect, the present invention provides a composition containing sheep placenta peptides, which comprises the following components by mass percentage: 8%-16% sheep placenta peptides, 2%-5% ergothioneine, and 3%-10% a first plant extract, wherein the first plant extract is a ebony lotus seed extract.

[0009] Preferably, the sheep placenta peptide-containing composition further comprises 1-5% by mass of a second plant extract, which is different from the first plant extract. Preferably, the second plant extract is a lemon extract and / or a mint extract.

[0010] In another aspect, the present invention provides a cosmetic comprising the aforementioned sheep placenta peptide-containing composition.

[0011] In another aspect, the present invention also provides a process for preparing the aforementioned composition containing sheep placental peptide, comprising the following steps:

[0012] a) dissolving the sheep placenta peptide in water, stirring evenly, and heating to 80-85°C;

[0013] b) cooling the solution obtained in step a) to 40-45° C., adding the ergothioneine and the ebony lotus seed extract, stirring well, and cooling to room temperature. DETAILED DESCRIPTION OF THE INVENTION

[0015] The present invention provides a composition containing sheep placenta peptide and other specific components. The applicant has found that when the sheep placenta peptide is combined with the specific components required by the present application at a certain mass percentage, the formed composition can achieve more complete absorption of the active ingredients in the sheep placenta peptide by the human body, thereby achieving better efficacy when applied to cosmetics.

[0016] The aforementioned "specific component" includes thioneine and the first plant extract of a specific mass percentage, and it is particularly worth mentioning that the first plant extract, i.e., ebony lotus seed extract. Not subject to theoretical constraints, the applicant is surprised to find that the ebony extract component in the ebony lotus seed extract can activate the skin, thereby enhancing the absorption of active ingredients in sheep placenta peptides and promoting skin metabolism; and the lotus seed extract component therein includes polyphenols, has a strong antioxidant capacity, and cooperates with thioneine, which is also an antioxidant in the composition, to effectively remove free radicals during skin metabolism and inhibit skin peroxidation. Therefore, the ebony lotus seed extract and thioneine in the present composition can jointly promote the small and medium molecule peptides contained in the sheep placenta peptides to be more effectively and fully absorbed by human body.

[0017] Composition containing sheep placenta peptide

[0018] The invention provides a composition containing sheep placenta peptides. The composition comprises the following components by mass percentage: 8%-16% of sheep placenta peptides, 2%-5% of ergothioneine, and 3%-10% of a first plant extract, wherein the first plant extract is a ebony lotus seed extract.

[0019] The composition of the present invention contains 8%-16% sheep placenta peptide, preferably 10-16%, more preferably 12-15%. Sheep placenta peptide is a natural biological component extracted from sheep embryos through biological enzyme hydrolysis, rich in immunoglobulins, cell growth factors, multiple enzymes and other effective ingredients, can enhance cell metabolism, and play an anti-aging effect.

[0020] In some preferred embodiments, 90% of the peptide molecular weight distribution of the sheep placenta peptide is below 1000 Daltons, more preferably below 500 Daltons. The peptide molecular weight distribution can be detected by any conventional, applicable method, preferably by the peptide molecular weight detection method described below, i.e., the method in Appendix A of GB / T22492-2008.

[0021] The composition of the present invention also contains 3%-10% of the first plant extract, i.e., ebony and lotus seed extract. Ebony plum and lotus seed are two ingredients that are less used in cosmetics at present. Without being bound by theory, the ebony extract is made from pitted ebony plums, which has a strong inhibitory effect on the catalytic enzyme (tyrosinase) produced by melanin, and can effectively prevent skin pigmentation by affecting melanin synthesis and reducing ultraviolet rays to promote melanin production; the lotus seed extract is a lotus seed with shell, containing alkaloids, flavonoids and polysaccharides, and has an antioxidant effect. The applicant was surprised to find that after adding a specific amount of ebony and lotus seed extract, its synergistic effect with small molecules in sheep placenta peptide can effectively promote the absorption of small molecule peptides in sheep placenta peptide by the human body. In some preferred embodiments, the aforementioned first plant extract accounts for 5-8% of the mass percentage of the composition.

[0022] The composition of the present invention also comprises 2%-5% of ergothioneine, preferably 4-5%. The active ingredient of ergothioneine is trimethyl mercaptohistidine inner salt, which belongs to natural water-soluble sulfur-containing amino acid and is a stable strong antioxidant. After being taken in and accumulated by the human body, ergothioneine can remove free radicals, chelate divalent metal cations, and has antioxidant and anti-inflammatory effects. The applicant was surprised to find that after adding a specific amount of ergothioneine, it is used in coordination with the antioxidant components in sheep placenta peptide and ebony lotus seed extract, which can further reduce the oxidation of active ingredients such as growth factors in sheep placenta peptide, and enhance whitening effect.

[0023] In some embodiments, the composition containing sheep placenta peptides further comprises a second plant extract different from the first plant extract at a mass percentage of 1-5%, and the second plant extract is preferably a lemon extract and / or a mint extract. The lemon extract mainly contains vitamin C, citric acid, lemon polyphenols and natural essential oils, and has antioxidant and antibacterial effects. The mint extract mainly contains substances such as flavonoids, amino acids, terpenes and natural essential oils, and has antioxidant, antibacterial, analgesic, anti-inflammatory, and antiviral effects. In some preferred embodiments, the second plant extract accounts for 2-4% of the mass percentage of the composition.

[0024] In some more preferred embodiments, the second plant extract is lemon extract and mint extract, and the ratio of the lemon extract to the mint extract in the composition is (5-10): 1, and the more preferred ratio is (6-8): 1. The applicant has found that at this ratio, the active ingredients of the lemon extract and the mint extract can interact with each other, achieving an effective bactericidal and antioxidant effect without excessively irritating human skin.

[0025] In other embodiments, the composition containing sheep placenta peptides further comprises the following components by mass percentage: 3-15% squalane, 2-7% butylene glycol, 2-7% sorbitan olivate, 1-5% hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and 0.1-1% phenoxyethanol. Among them, squalane is prepared by hydrogenation of squalene, has excellent antioxidant, thermal stability and chemical stability, has good affinity to the skin, does not cause allergies and irritation, and can accelerate the penetration of other active ingredients in the composition into the skin; butylene glycol is a heat preservation agent and solvent, which can prevent water loss; sorbitan olivate is an emulsifier and dispersant; hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer is an emulsifier and thickener; phenoxyethanol is a preservative. Preferably, the sheep placenta peptide-containing composition further comprises, by mass percentage: 5-10% squalane, 3-5% butylene glycol, 3-5% sorbitan olivate, 2-3% hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and 0.4-0.6% phenoxyethanol.

[0026] In a highly preferred embodiment, the composition containing sheep placenta peptides comprises the following components by mass percentage: 8%-16% sheep placenta peptides, 2%-5% ergothioneine, 3%-10% first plant extract, 1%-5% second plant extract, 3%-15% squalane, 2%-7% butylene glycol, 2%-7% sorbitan olive oil ester, 1%-5% hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and 0.1%-1% phenoxyethanol. Further preferably, the first plant extract is ebony lotus seed extract, and the second plant extract is lemon extract and mint extract, and the ratio of the two in the composition is (5-10):1.

[0027] Preparation process

[0028] The present invention provides a preparation process of a composition containing sheep placenta peptide, comprising the following steps:

[0029] a) dissolving the sheep placenta peptide in water, stirring evenly, and heating to 80-85°C;

[0030] b) cooling the solution obtained in step a) to 40-45° C., adding the ergothioneine and the ebony lotus seed extract, stirring well, and cooling to room temperature.

[0031] When preparing a preferred composition comprising a second plant extract, the second plant extract may be added in the aforementioned step a), or may be added in the aforementioned step b), or a suitable adding step may be selected according to the specific components of the second plant extract. In some embodiments, when the second plant extract comprises lemon extract and mint extract, the aforementioned preparation process correspondingly comprises the following steps:

[0032] a) dissolving the sheep placenta peptide and lemon extract in water, stirring evenly, and heating to 80-85° C.;

[0033] b) cooling the solution obtained in step a) to 40-45° C., adding the ergothioneine, the ebony lotus seed extract and the peppermint extract, stirring well, and cooling to room temperature.

[0034] When preparing a preferred composition comprising the aforementioned other components, that is, when the composition further comprises squalane, butylene glycol, sorbitan olivate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer and phenoxyethanol, the aforementioned other components are preferably added in steps a) and b) according to the specific characteristics of the components. In some embodiments, the process for preparing a preferred composition comprising the aforementioned other components comprises the following steps:

[0035] a1) adding water to dissolve the sheep placenta peptide, the butanediol and the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 80-85° C.;

[0036] a2) heating the squalane and the sorbitan olivate to 80-85° C. and stirring evenly to form an oil phase mixture;

[0037] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 80-85° C. for emulsification;

[0038] a4) cooling the solution obtained in step a3) to 40-45° C., and then heating it again to 80-85° C. for secondary emulsification; and

[0039] b) cooling the solution obtained in step a4) to 40-45° C., adding the ergothioneine, the ebony lotus seed extract, the squalane and the phenoxyethanol, stirring well, and cooling to room temperature.

[0040] When preparing a composition comprising the aforementioned second plant extract and the aforementioned other component substances, that is, when the composition further comprises lemon extract, mint extract, squalane, butylene glycol, sorbitan olivate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer and phenoxyethanol, the aforementioned component substances can preferably be added in a specific step according to the properties of the substances, for example, each component is added in steps a1), a2), and b) respectively. In some embodiments, the preparation process of the composition of the aforementioned components comprises the following steps:

[0041] a1) adding water to dissolve the sheep placenta peptide, lemon extract, butylene glycol and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer to form an aqueous phase mixture by stirring evenly, and heating to 80-85° C.;

[0042] a2) heating the squalane and the sorbitan olivate to 80-85° C. and stirring evenly to form an oil phase mixture;

[0043] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 80-85° C. for emulsification;

[0044] a4) cooling the solution obtained in step a3) to 40-45° C., and then heating it again to 80-85° C. for secondary emulsification; and

[0045] b) cooling the solution obtained in step a4) to 40-45° C., adding the ergothioneine, the ebony lotus seed extract, the peppermint extract, the squalane and the phenoxyethanol, stirring well, and cooling to room temperature.

[0046] In the above preparation process, by mixing the aqueous phase and the oil phase in steps a1) and a2) respectively, and emulsifying twice in steps a3) and a4), the components are distributed more evenly and stably in the prepared sheep placenta peptide-containing composition, and the prepared uniform and stable composition is more easily absorbed by the human body.

[0047] The aforementioned ebony lotus seed extract can be prepared by any conventional method, or can be purchased commercially. In some preferred embodiments, the preparation method of the aforementioned ebony lotus seed extract comprises:

[0048] a) removing the pits of dark plums and crushing the flesh to make dark plum powder, and crushing lotus seeds with shells to make lotus seed powder; and

[0049] b) Weigh the dark plum powder and lotus seed powder in a weight ratio of 2-4:1, add 5-15 times 70% ethanol solution, reflux extraction for 1-2h, and then filter through a filter with a pore size of 1-4mm, and use a rotary evaporator to concentrate the filtrate to 0.5-2g / ml at a rotary evaporation pressure of 0.01-0.05MPa and a rotary evaporation temperature of 50-70°C. More preferably, weigh the dark plum powder and lotus seed powder in a weight ratio of 2:1, add 10 times 70% ethanol solution, reflux extraction for 1.5h, filter with a pore size of 1mm, and use a rotary evaporator to concentrate the filtrate to 1g / ml at a rotary evaporation pressure of 0.01MPa and a rotary evaporation temperature of 60°C. This preparation method can achieve a balance between the preparation effect and efficiency of dark plum and lotus seed extracts, and while obtaining a purer extract, it makes the dark plum and lotus seed extracts easier to mix with other components of the composition, and the preparation efficiency is higher.

[0050] The lemon extract can be prepared in any conventional manner or can be purchased commercially. In some preferred embodiments, the preparation method of the lemon extract comprises:

[0051] a) Wash fresh lemons and squeeze out the juice;

[0052] b) after high-speed shearing of the pulp and peel after juicing, 5-15 times of pure water is added and filtered through 50-100 mesh, preferably 100 mesh gauze, and the filtrate is collected;

[0053] c) mixing lemon juice with the filtrate obtained in step b), adding acidic cellulase and pectinase, adjusting the pH to about 5-6 with sodium hydroxide solution, heating to 50-60° C., performing enzymolysis for 1-2 hours, preferably for 2 hours, heating to 80-90° C., preferably 90° C., maintaining for 20-30 minutes to inactivate the enzyme, preferably for 30 minutes, and filtering through a filter cloth with a mesh size of 150-200, preferably 200; and

[0054] d) using a vacuum rotary evaporator at a rotary evaporation pressure of 0.01-0.05 MPa, preferably 0.01 MPa, and a rotary evaporation temperature of 60-80° C., preferably 80° C., to concentrate the filtrate to 0.5-2 g / ml, preferably 1 g / ml.

[0055] The above-mentioned peppermint extract can be prepared by any conventional method, or can be purchased commercially. In some preferred embodiments, the preparation method of the above-mentioned peppermint extract comprises:

[0056] a) crushing fresh mint, adding 5-10 times 70% ethanol solution, reflux extraction for 1-2h, and filtering with a coarse filter membrane; and

[0057] b) using a rotary evaporator to concentrate the filtrate to 0.5-2g / ml at a rotary evaporation pressure of 0.01-0.05MPa and a rotary evaporation temperature of 50-70°C. More preferably, fresh mint is crushed, 10 times of 70% ethanol solution is added, and after reflux extraction for 1.5h, it is filtered through a coarse filter membrane, and the filtrate is concentrated to 1g / ml at a rotary evaporation pressure of 0.01MPa and a rotary evaporation temperature of 60°C using a rotary evaporator. Wherein "reflux extraction" refers to using a reflux condenser to evaporate the ethanol solution of the target substance (i.e., mint) and then condense and reflux to the reaction vessel. This process can increase the reaction time and improve the extraction efficiency, and is a method suitable for extracting the mint extract of the present invention. This preparation method not only obtains a purer mint extract and makes the mint extract easier to mix with other components of the composition, but also has a higher preparation efficiency.

[0058] Application of composition containing sheep placenta peptide

[0059] The present invention provides a cosmetic comprising the above-mentioned sheep placenta peptide-containing composition. The cosmetic can be any known cosmetic on the market, as long as it is suitable for adding the sheep placenta peptide-containing composition of the present invention.

[0060] When used in cosmetics, the composition of the present invention can be directly used as a finished product, or other suitable components can be added to form a final finished product. In some embodiments, the aforementioned cosmetics are skin care products, facial mask products, facial essences, facial cleansing products, preferably facial skin care products or eye creams, more preferably eye creams.

[0061] Peptide molecular weight detection method

[0062] Any conventional peptide content detection method can be used to detect the peptide content of the composition containing sheep placental peptide prepared by the present invention, preferably the method in Appendix A of GB / T 22492-2008 is used to detect the content of peptides with a molecular weight of <500D. This standard is included in the national standard full text disclosure system, see https: / / openstd.samr.gov.cn / bzgk / gb / newGblnfo?hcno=80B693FE7FFFA345807FF3D1AA185DC9. DETAILED DESCRIPTION

[0063] The examples herein are intended to illustrate the invention but are not intended to limit or otherwise define the scope of the invention.

[0064] Examples 1-2 are examples of the sheep placenta peptide composition of the present invention, Examples 3-4 are comparative examples, Examples 5-9 are preparation methods of each example and comparative example, and Example 10 is experimental data of the application effects of each example and comparative example.

[0065] Example 1-2, Comparative Example 3-4: Composition containing sheep placenta peptide

[0066] The compositions shown in Table 1 consist of the listed ingredients in the listed mass percentages.

[0067] Table 1

[0068]

[0069]

[0070] Example 5: Preparation method of the composition of Example 1

[0071] a1) adding water to dissolve sheep placental peptide, butylene glycol and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 85° C.;

[0072] a2) heating squalane and sorbitan olivate to 85° C. and stirring evenly to form an oil phase mixture;

[0073] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 85° C. for emulsification;

[0074] a4) cooling the solution obtained in step a3) to 45° C., and then heating it again to 85° C. for secondary emulsification; and

[0075] b) cooling the solution obtained in step a4) to 45° C., adding ergothioneine, ebony lotus seed extract and phenoxyethanol, stirring well, and cooling to room temperature.

[0076] Example 6: Preparation method of the composition of Example 2

[0077] a1) adding water to dissolve sheep placenta peptide, lemon extract, butylene glycol and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 85° C.;

[0078] a2) heating squalane and sorbitan olivate to 85° C. and stirring evenly to form an oil phase mixture;

[0079] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 85° C. for emulsification;

[0080] a4) cooling the solution obtained in step a3) to 45° C., and then heating it again to 85° C. for secondary emulsification; and

[0081] b) cooling the solution obtained in step a4) to 45° C., adding ergothioneine, ebony lotus seed extract, peppermint extract and phenoxyethanol, stirring well, and cooling to room temperature.

[0082] Example 7: Preparation method of the composition of comparative example 3

[0083] a1) adding water to dissolve sheep placental peptide, butylene glycol and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 85° C.;

[0084] a2) heating squalane and the aforementioned sorbitan olivate to 85° C. and stirring evenly to form an oil phase mixture;

[0085] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 85° C. for emulsification;

[0086] a4) cooling the solution obtained in step a3) to 45° C., and then heating it again to 85° C. for secondary emulsification; and

[0087] b) cooling the solution obtained in step a4) to 45° C., adding ergothioneine and phenoxyethanol, stirring well, and cooling to room temperature.

[0088] Example 8: Preparation method of the composition of Comparative Example 4

[0089] a1) adding water to dissolve sheep placental peptide, butylene glycol and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 85° C.;

[0090] a2) heating the aforementioned squalane and sorbitan olivate to 85° C. and stirring evenly to form an oil phase mixture;

[0091] a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 85° C. for emulsification;

[0092] a4) cooling the solution obtained in step a3) to 45° C., and then heating it again to 85° C. for secondary emulsification; and

[0093] b) cooling the solution obtained in step a4) to 45° C., adding ergothioneine, ebony lotus seed extract and phenoxyethanol, stirring well, and cooling to room temperature.

[0094] Example 9: Comparative preparation method of the composition of Example 2

[0095] All the components of the composition of Example 2, namely, sheep placental peptide, ergothioneine, squalane, ebony lotus seed extract, lemon extract, mint extract, butylene glycol, sorbitan olivate, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, phenoxyethanol and water, were directly mixed and stirred evenly according to the mass percentages in Table 1, and heated to 85°C.

[0096] The components of the composition prepared by the aforementioned Example 9 were not mixed uniformly enough, the product was not fully emulsified, and the obtained solution had stratification phenomenon, so the composition produced by this preparation method was unusable.

[0097] Example 10: Application effect experimental data

[0098] 40 female volunteers aged 30 years and above (excluding pregnant or lactating women) were selected as subjects. Inclusion criteria: 1. Volunteers have not applied other eye drops in the past 2 months; 2. The skin at the corners of the eyes lacks elasticity and has obvious fine lines; 3. There are no interference factors that obviously affect the test results in the test area, such as birthmarks, acne, scratches, moles, etc.; 4. Can cooperate with the test seriously and return to the hospital on time.

[0099] The above population was randomly divided into 4 groups, 10 people in each group, and the products of Examples 1 and 2 and Comparative Examples 3 and 4 prepared in Examples 5-8 were used respectively. The subjects first sat quietly for 30 minutes to allow the skin to reach a balanced state, and then applied the corresponding composition around the eyes (i.e., the composition was used as an eye cream), evenly applied at (1.0±0.1) mg / cm2, and gently pressed for absorption. During the trial, the subjects used it once a day and could not use other eye creams. A follow-up visit was conducted after 8 weeks of continuous use.

[0100] The skin L* value was recorded using a 3nh brand CR4501 series skin color tester (the L* value indicates brightness, and the larger the value, the more it tends to be white). A fixed area was measured each time, and the average value was taken for 3 measurements. The average value of the measurements of 10 subjects in each group was then taken. The results are shown in Table 2 below.

[0101] Table 2

[0102] project 0 Weeks 8 weeks Difference between 8 weeks and 0 weeks Overall improvement ratio Example 1 55.71±1.96 56.8±1.98 1.09 1.95％ Example 2 55.72±2.37 56.89±2.33 1.17 2.10％ Comparative Example 3 55.69±1.75 56.36±1.74 0.67 1.20％ Comparative Example 4 55.66±1.66 56.69±1.69 1.03 1.85％

[0103] From the above test results, it can be seen that the skin L* value is the lowest at 0 weeks, and the values ​​of each group are not much different, that is, the grouping is relatively uniform. After 8 weeks of use, the L* values ​​have increased to varying degrees, but Examples 1 and 2 under the present invention have significantly better whitening effects than Comparative Examples 3 and 4. Furthermore, Example 2 achieves the best whitening effect because the content and ratio of its components reach the optimal setting.

[0104] Although specific embodiments of the present invention have been described and illustrated, it will be appreciated by those skilled in the art that various other changes and modifications may be made without departing from the spirit and scope of the present invention. Therefore, all such changes and modifications are intended to fall within the scope of the claims of this application.

[0105] All percentages, ratios and proportions are calculated by weight of the total composition unless otherwise indicated. All temperatures are in degrees Celsius (° C.) unless otherwise indicated. All component or composition levels refer to the active level of that component or composition, and are exclusive of impurities, such as residual solvents or by-products, which may be present in commercially available sources.

[0106] It should be understood that each numerical upper limit given in this specification includes values ​​lower than it, just as if these lower values ​​are explicitly written in the application. Each numerical lower limit given in this specification includes values ​​higher than it, just as if these higher values ​​are explicitly written in the application. Each numerical range given in this specification includes narrower numerical ranges falling within the numerical range, just as if these narrower numerical ranges are explicitly written in the application.

[0107] The values ​​and dimensions disclosed herein should not be understood as being strictly limited to the exact values ​​recited. Instead, unless otherwise specified, each such value and dimension is intended to represent the recited value and a functionally equivalent range around that value. For example, a dimension disclosed as "40 millimeters" is intended to mean "about 40 millimeters."

[0108] As used herein, the term "mixing" refers to adding ingredients together and achieving homogeneity, and the term "mixture" refers to a homogeneous mixture of ingredients.

[0109] In the present application, when a composition is “substantially free” of a specific ingredient, it means that the composition contains less than a trace amount, or less than 0.1%, or less than 0.01%, or less than 0.001%, based on the mass of the composition, of the specific ingredient.

[0110] In the present application, the terms "a)", "b)", "b1)", "b2)" and "c)" used to refer to the steps in the preparation method do not indicate the order unless the order is particularly clear. For example, step a) may occur before step b) or after step b), or steps b) and c) may occur simultaneously, or step b) may occur after step c).

[0111] The terms "comprising", "including", "comprising", "including", "comprising", "including", "containing", "including" and "containing" used in this application are intended to be non-limiting, that is, other ingredients and other steps that do not affect the end result may be added.

Claims

1. A composition containing sheep placenta peptide, comprising the following components by mass percentage: 8%-16% Sheep Placenta Peptide, 2%-5% ergothioneine, and 3%-10% of a first plant extract, wherein the first plant extract is a ebony lotus seed extract.

2. The composition containing sheep placental peptide according to claim 1, wherein 90% of the peptide molecular weight distribution of the sheep placental peptide is below 500 Daltons.

3. The sheep placenta peptide-containing composition according to claim 1, further comprising 1-5% by mass of a second plant extract, wherein the second plant extract is a lemon extract and / or a mint extract.

4. The sheep placenta peptide-containing composition according to claim 3, wherein the second plant extract is lemon extract and mint extract, and the ratio of the lemon extract to the mint extract in the composition is (5-10):

1.

5. The composition containing sheep placenta peptide according to claim 1, further comprising the following components by mass percentage: 3-15% squalane, 2-7% butylene glycol, 2-7% sorbitan olivate, 1-5% hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and 0.1-1% phenoxyethanol.

6. A cosmetic comprising the sheep placenta peptide-containing composition according to any one of claims 1 to 5.

7. The cosmetic according to claim 6, which is a facial skin care product or an eye cream.

8. A process for preparing the composition containing sheep placental peptide according to any one of claims 1 to 5, comprising the following steps: a) adding water to dissolve the sheep placenta peptide, stirring evenly, and heating to 80-85° C.; b) cooling the solution obtained in step a) to 40-45° C., adding the ergothioneine and the ebony lotus seed extract, stirring well, and cooling to room temperature.

9. A process for preparing the composition containing sheep placental peptide according to claim 4, comprising the following steps: a) adding water to dissolve the sheep placenta peptide and the lemon extract, stirring evenly, and heating to 80-85° C.; b) cooling the solution obtained in step a) to 40-45° C., adding the ergothioneine, the extract of the dark plum and lotus seeds and the peppermint extract, stirring well, and cooling to room temperature.

10. A process for preparing the composition containing sheep placental peptide according to claim 5, comprising the following steps: a1) adding water to dissolve the sheep placental peptide, the butanediol and the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, stirring evenly to form an aqueous phase mixture, and heating to 80-85° C.; a2) heating the squalane and the sorbitan olivate to 80-85° C. and stirring evenly to form an oil phase mixture; a3) mixing the aqueous phase mixture obtained in step a1) with the oil phase mixture obtained in step a2), stirring evenly, and heating to 80-85° C. for emulsification; a4) cooling the solution obtained in step a3) to 40-45° C., and then heating it again to 80-85° C. for secondary emulsification; and b) cooling the solution obtained in step a4) to 40-45° C., adding the ergothioneine, the ebony lotus seed extract, the squalane and the phenoxyethanol, stirring well, and cooling to room temperature.

11. The preparation process according to claim 8, wherein the preparation method of the ebony lotus seed extract comprises: a) removing the core of the dark plums, crushing the flesh to make dark plum powder, and crushing the lotus seeds with shells to make lotus seed powder; and b) Weigh black plum powder and lotus seed powder in a weight ratio of 2-4:1, add 5-15 times 70% ethanol solution, reflux extraction for 1-2h, and then filter through a filter with a pore size of 1-4mm, and use a rotary evaporator to concentrate the filtrate to 0.5-2g / ml at a rotary evaporation pressure of 0.01-0.05MPa and a rotary evaporation temperature of 50-70°C.

12. The preparation process according to claim 9, wherein the preparation method of the lemon extract comprises: a) Wash fresh lemons and squeeze out the juice; b) After the pulp and peel after juicing are subjected to high-speed shearing, 5-15 times of pure water are added, filtered through 50-100 mesh gauze, and the filtrate is collected; c) mixing lemon juice with the filtrate obtained in step b), adding acidic cellulase and pectinase, adjusting the pH to about 5-6 with sodium hydroxide solution, heating to 50-60° C., performing enzymolysis for 1-2 hours, heating to 80-90° C. for 20-30 minutes to inactivate the enzyme, and filtering through a 150-200 mesh filter cloth; and d) Using a vacuum rotary evaporator at a rotary evaporation pressure of 0.01-0.05 MPa and a rotary evaporation temperature of 60-80° C., the filtrate was concentrated to 0.5-2 g / ml.

13. The preparation process according to claim 9, wherein the preparation method of the peppermint extract comprises: a) Crush fresh mint, add 5-10 times 70% ethanol solution, reflux extraction for 1-2 hours, and filter using a coarse filter membrane; and b) Using a rotary evaporator, the filtrate is concentrated to 0.5-2 g / ml at a rotary evaporation pressure of 0.01-0.05 MPa and a rotary evaporation temperature of 50-70°C.

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