A kind of anti-oxidation and anti-aging Chinese medicine composition and its preparation method and application

Abstract

The present invention belongs to the technical field of cosmetics, and discloses a kind of Chinese medicine composition of anti-oxidation and anti-aging, and its preparation method and application. The Chinese medicine raw materials used in the Chinese medicine composition of the present invention include 10-60 parts of Radix Pseudostellariae, 10-40 parts of Radix Vaccariae, 10-40 parts of Radix Rubiae and 10-40 parts of Radix Asterae. Wherein Radix Asterae has a strong effect of removing DPPH free radicals, Radix Rubiae and Radix Asterae can better inhibit inflammatory factors IL-6 and TNF-α, and in promoting the generation of collagen and elastin, Radix Pseudostellariae and Radix Vaccariae have more advantages; The present invention is by scientifically and rationally compounding Radix Pseudostellariae, Radix Vaccariae, Radix Rubiae and Radix Asterae, four kinds of Chinese medicine raw materials synergistically act, promote each other, the obtained Chinese medicine composition can remove free radicals, reduce inflammatory factors and improve type I collagen and elastin content, through multi-pathway action, can effectively improve skin elasticity and wrinkles, can achieve the purpose of effective anti-aging.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an anti-oxidation and anti-aging Chinese medicine composition and a preparation method and application thereof. Background Art

[0002] Skin aging is an inevitable stage that accompanies human life activities. It is affected by genetic factors, environmental factors, and the complex morphological structure of the whole body. In recent years, the mechanism of aging has been continuously explored, and different aging theories have been proposed in different dimensions. Among them, the more representative ones are: free radical aging theory, non-enzymatic glycosylation aging theory, gene regulation theory, mitochondrial theory, etc. Aging is a complex and continuous biological process. For example, the metabolic function of the skin declines, the function of the antioxidant system in the body declines, the activity of antioxidant enzymes continues to decrease, free radicals accumulate excessively, and clearance obstacles occur; in addition, long-term excessive exposure to ultraviolet rays can also cause oxidative stress, inflammatory response, telomere damage, and reduced collagen synthesis, causing aging damage and accelerating skin aging. Aging skin is mainly manifested as: reduced skin moisture content, reduced elasticity, and wrinkles.

[0003] At present, most of the products on the market that claim to have anti-aging effects only use the method of removing free radicals or increasing the collagen content of the skin. This single-dimensional anti-aging approach often has poor effects and the anti-aging effect does not meet expectations. Therefore, it is very necessary to develop a product that can effectively fight against aging through multiple pathways. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides an anti-oxidative and anti-aging Chinese medicine composition and its preparation method and application. The Chinese medicine composition provided by the present invention can remove free radicals, reduce inflammatory factors and increase the content of type I collagen and elastin. Through multi-pathway effects, it can effectively improve skin elasticity and wrinkles, and can achieve the purpose of effective anti-aging.

[0005] The first aspect of the present invention provides a traditional Chinese medicine composition, wherein the traditional Chinese medicine raw materials used in the traditional Chinese medicine composition include 10-60 parts of Pseudostellariae Radix, 10-40 parts of Vaccariae Semen, 10-40 parts of Rubia cordifolia and 10-40 parts of Aster tataricus in parts by weight.

[0006] Specifically, Pseudostellaria heterophylla is the dried root of the plant Pseudostellaria heterophylla of the Caryophyllaceae family, which is rich in amino acids, cyclic peptides, triterpenoid saponins, polysaccharides and other ingredients. Among them, Pseudostellaria heterophylla cyclic peptide B is one of the main ingredients of the traditional Chinese medicine Pseudostellaria heterophylla, and is also one of the most studied cyclic peptide ingredients of Pseudostellaria heterophylla. Pseudostellaria heterophylla cyclic peptide B has the effects of promoting collagen production and scavenging free radicals.

[0007] Specifically, Wangbuliuxing is the dried mature seeds of the Caryophyllaceae plant, which mainly contains chemical components such as cyclopeptides, flavonoid glycosides, alkaloids and volatile oils, and has pharmacological effects such as antibacterial, anti-inflammatory and antioxidant. Eight cyclopeptides have been isolated from it, namely Wangbuliuxing cyclopeptide AH, among which Wangbuliuxing cyclopeptide B is one of the characteristic components of Wangbuliuxing.

[0008] Specifically, madder is a traditional hemostatic drug with the effects of cooling blood, stopping bleeding, removing phlegm, and promoting menstruation. The main chemical components of madder plants are water-soluble cyclohexyl peptides, fat-soluble anthraquinones and their glycosides, reduced naphthoquinones and their glycosides, and also contain polysaccharides, terpenes, trace elements, madderin, madder acid, etc., which have anti-inflammatory, antioxidant, and antibacterial effects.

[0009] Specifically, Aster is the dried root of a perennial herb of the genus Aster in the Asteraceae family. It is a traditional and commonly used Chinese medicinal material with a long history of medicinal use. It has a slight fragrance and a sweet and slightly bitter taste. Its main chemical components include terpenes, peptides, coumarins, flavonoids, anthraquinones, etc. It has good antioxidant, antiviral, and anti-tumor pharmacological effects. Among them, peptides are a more distinctive class of chemical components in Aster.

[0010] In some embodiments of the present invention, the Chinese medicinal raw materials used in the Chinese medicine composition include 20-50 parts of Pseudostellariae Radix, 10-30 parts of Vaccariae Semen, 10-30 parts of Rubia cordifolia and 10-30 parts of Aster tataricus, measured by weight.

[0011] The second aspect of the present invention provides a method for preparing a Chinese medicine composition, comprising the following steps:

[0012] S1, mixing Pseudostellaria heterophylla, Rubia cordifolia, Aster tataricus and a solvent, and homogenizing to obtain a mixed solution;

[0013] S2, heating the mixed solution to extract and obtain an extract;

[0014] S3. Mix the extract with Vaccaria segetalis, homogenize, filter and concentrate to obtain a Chinese medicine composition.

[0015] In some embodiments of the present invention, before performing step S1, the method further includes the steps of: crushing and sieving Pseudostellariae Radix, Vaccariae Semen, Rubia cordifolia and Aster tataricus respectively in advance.

[0016] In some embodiments of the present invention, the mesh size of the sieving is 10-50 meshes.

[0017] In some embodiments of the present invention, the solvent comprises water or a polyol.

[0018] In some embodiments of the present invention, in step S1, the homogenization speed is 1000-8000 rpm, and the homogenization time is 10-60 min.

[0019] In some embodiments of the present invention, in step S1, the homogenization speed is 4000-6000 rpm, and the homogenization time is 20-40 min.

[0020] In some embodiments of the present invention, in step S3, the homogenization speed is 1000-8000 rpm, and the homogenization time is 20-90 min.

[0021] In some embodiments of the present invention, in step S3, the homogenization speed is 4000-6000 rpm, and the homogenization time is 50-70 min.

[0022] In some embodiments of the present invention, the temperature of the heating extraction is 50-100° C.; and / or the number of the heating extraction is 2-3 times; and / or the time of the heating extraction is 1-3 hours.

[0023] In some embodiments of the present invention, the ratio of the total mass of Pseudostellariae Radix, Rubia cordifolia and Aster tataricus to the mass of the solvent is 1:(8-15).

[0024] In some embodiments of the present invention, the ratio of the total mass of Pseudostellariae Radix, Rubia cordifolia and Aster tataricus to the mass of the solvent is 1:(8-12).

[0025] In some embodiments of the present invention, the concentration is: concentrating the filtered filtrate to a ratio of 1:(1-5) to the total mass of the Chinese medicine raw material.

[0026] The third aspect of the present invention provides a cosmetic, which comprises the traditional Chinese medicine composition described in the first aspect of the present invention or the traditional Chinese medicine composition prepared by the preparation method described in the second aspect of the present invention.

[0027] In some embodiments of the present invention, the content of the traditional Chinese medicine composition in the cosmetic is 0.01wt-10wt%.

[0028] In some embodiments of the present invention, the content of the traditional Chinese medicine composition in the cosmetic is 0.01wt-5wt%.

[0029] In some embodiments of the present invention, the content of the traditional Chinese medicine composition in the cosmetic is 0.1wt-2wt%.

[0030] In some embodiments of the present invention, the dosage form of the cosmetic includes any one of an aqueous solution, an emulsion, a spray, a cream, an essence or a mask.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] The Chinese medicine composition of the invention adopts Chinese medicines such as Radix Pseudostellariae, Radix Vaccariae, Rubia cordifolia and Aster tataricus as preparation raw materials, wherein Aster tataricus has a strong effect of scavenging DPPH free radicals, Rubia cordifolia and Aster tataricus can better inhibit inflammatory factors IL-6 and TNF-α, and Radix Pseudostellariae and Radix Vaccariae are more advantageous in promoting the production of collagen and elastin. The invention scientifically and reasonably combines Radix Pseudostellariae, Radix Vaccariae, Rubia cordifolia and Aster tataricus, and the four Chinese medicine raw materials act synergistically and promote each other, so that the obtained Chinese medicine composition can scavenge free radicals, reduce inflammatory factors and increase the content of type I collagen and elastin, and can effectively improve skin elasticity and wrinkles through multi-pathway effects, so as to achieve the purpose of effective anti-aging. DETAILED DESCRIPTION

[0033] The present invention is further described in detail below by specific examples. The raw materials, reagents or devices used in the examples can be obtained from conventional commercial sources or by prior art methods unless otherwise specified. Unless otherwise specified, the experiments or test methods are conventional methods in the art.

[0034] Example 1

[0035] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Pseudostellaria heterophylla, 20g of Vaccaria segetalis, 20g of Rubia cordifolia and 20g of Aster tataricus.

[0036] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0037] (1) Grind Radix Pseudostellariae, Radix Vaccariae, Radix Rubiae and Radix Asteris respectively, pass through a 20-mesh sieve, and set aside;

[0038] (2) mixing the crushed and sieved Radix Pseudostellariae, Radix Rubiae and Rhizoma Asteris in step (1), and then mixing with water in a mass ratio of 1:10, and then homogenizing at 6000 rpm for 30 min to obtain a mixed solution;

[0039] (3) heating the mixed solution obtained in step (2) at 80° C. for extraction twice, each time for 1 h, to obtain an extract;

[0040] (4) The extract obtained in step (3) is mixed with the crushed and sieved fenugreek in step (1), and the mixture is homogenized and extracted at 6000 rpm for 60 min. The mixture is filtered and the filtrate is concentrated to 100 g to obtain a traditional Chinese medicine composition.

[0041] Example 2

[0042] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 30g of Pseudostellaria heterophylla, 10g of Vaccaria segetalis, 25g of Rubia cordifolia and 15g of Aster tataricus.

[0043] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0044] (1) Grind Radix Pseudostellariae, Radix Vaccariae, Radix Rubiae and Radix Asteris respectively, pass through a 20-mesh sieve, and set aside;

[0045] (2) mixing the crushed and sieved Radix Pseudostellariae, Radix Rubiae and Rhizoma Asteris in step (1), and then mixing with water in a mass ratio of 1:10, and then homogenizing at 6000 rpm for 30 min to obtain a mixed solution;

[0046] (3) heating the mixed solution obtained in step (2) at 80° C. for extraction twice, each time for 1 h, to obtain an extract;

[0047] (4) The extract obtained in step (3) is mixed with the crushed and sieved fenugreek in step (1), and the mixture is homogenized and extracted at 6000 rpm for 60 min. The mixture is filtered and the filtrate is concentrated to 100 g to obtain a traditional Chinese medicine composition.

[0048] Example 3

[0049] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 40g of Pseudostellaria heterophylla, 10g of Vaccaria segetalis, 15g of Rubia cordifolia and 25g of Aster tataricus.

[0050] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0051] (1) Grind Radix Pseudostellariae, Radix Vaccariae, Radix Rubiae and Radix Asteris respectively, pass through a 20-mesh sieve, and set aside;

[0052] (2) mixing the crushed and sieved Radix Pseudostellariae, Radix Rubiae and Rhizoma Asteris in step (1), and then mixing with water in a mass ratio of 1:10, and then homogenizing at 6000 rpm for 30 min to obtain a mixed solution;

[0053] (3) heating the mixed solution obtained in step (2) at 80° C. for extraction twice, each time for 1 h, to obtain an extract;

[0054] (4) The extract obtained in step (3) is mixed with the crushed and sieved fenugreek in step (1), and the mixture is homogenized and extracted at 6000 rpm for 60 min. The mixture is filtered and the filtrate is concentrated to 100 g to obtain a traditional Chinese medicine composition.

[0055] Comparative Example 1

[0056] A preparation method of a Pseudostellaria heterophylla extract comprises the following steps:

[0057] (1) Crush 20 g of Radix Pseudostellariae Radix and pass through a 20-mesh sieve for later use;

[0058] (2) mixing the crushed and sieved prince ginseng obtained in step (1) with water in a mass ratio of 1:10, and then homogenizing at 6000 rpm for 30 min to obtain a mixed solution;

[0059] (3) heating the mixed solution obtained in step (2) at 80° C. for extraction twice, each time for 1 h, to obtain an extract;

[0060] (4) Filter the extract obtained in step (3), and concentrate the filtrate to 100 g to obtain a Pseudostellaria heterophylla extract.

[0061] Comparative Example 2

[0062] A Vaccaria segetalis extract, the preparation method of which comprises the following steps:

[0063] (1) Crush 20 g of Vaccaria segetalis, pass through a 20-mesh sieve, and set aside;

[0064] (2) The crushed and sieved fenugreek in step (1) was mixed with water in a mass ratio of 1:10, homogenized and extracted at 6000 rpm for 60 min, filtered, and the filtrate was concentrated to 100 g to obtain a fenugreek extract.

[0065] Comparative Example 3

[0066] The difference from Comparative Example 1 is that 20 g of Pseudostellariae Radix is ​​replaced by 20 g of Rubia cordifolia, and the preparation method is the same as that of Comparative Example 1; in this example, a Rubia cordifolia extract is prepared.

[0067] Comparative Example 4

[0068] The difference from Comparative Example 1 is that 20 g of Pseudostellariae Radix is ​​replaced by 20 g of Aster ternata, and the preparation method is the same as that of Comparative Example 1; in this example, an Aster ternata extract is prepared.

[0069] Comparative Example 5

[0070] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Pseudostellaria heterophylla and 20g of Vaccaria segetalis.

[0071] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0072] Comparative Example 6

[0073] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Pseudostellaria heterophylla, 20g of Vaccaria segetalis and 20g of Rubia cordifolia.

[0074] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0075] Comparative Example 7

[0076] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Vaccaria segetalis and 20g of Rubia cordifolia.

[0077] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0078] Comparative Example 8

[0079] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Vaccaria segetalis, 20g of Rubia cordifolia and 20g of Aster ternata.

[0080] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0081] Comparative Example 9

[0082] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of madder and 20g of aster.

[0083] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0084] Comparative Example 10

[0085] A traditional Chinese medicine composition adopts traditional Chinese medicine raw materials including: 20g of Pseudostellaria heterophylla and 20g of Rubia cordifolia.

[0086] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0087] Comparative Example 11

[0088] A traditional Chinese medicine composition, wherein the traditional Chinese medicine raw materials used include: 20g of Pseudostellaria heterophylla and 20g of Aster ternata.

[0089] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0090] Comparative Example 12

[0091] A traditional Chinese medicine composition, wherein the traditional Chinese medicine raw materials used include: 20g of Vaccaria segetalis and 20g of Aster ternata.

[0092] The preparation method of the Chinese medicine composition of this example is the same as that of Example 1.

[0093] Comparative Example 13

[0094] A Chinese medicine composition, wherein the Chinese medicine raw materials used are the same as those in Example 1, and the preparation method thereof is different from that in Example 1 only in that "homogenization at 6000 rpm" in steps (2) and (4) is adjusted to "stirring at 500 rpm".

[0095] Comparative Example 14

[0096] A Chinese medicine composition, the Chinese medicine raw materials used in it are the same as those in Example 1, and the preparation method thereof comprises the following steps:

[0097] (1) Grind Radix Pseudostellariae, Radix Vaccariae, Radix Rubiae and Radix Asteris respectively, pass through a 20-mesh sieve, and set aside;

[0098] (2) mixing the crushed and sieved Radix Pseudostellariae Radix, Radix Rubiae Cordata, Rhizoma Asteris and Rhizoma Vaccariae in step (1), and then mixing with water in a mass ratio of 1:10, and then homogenizing at 6000 rpm for 30 min to obtain a mixed solution;

[0099] (3) heating the mixed solution obtained in step (2) at 80° C. for extraction twice, each time for 1 h, to obtain an extract;

[0100] (4) Filter the extract obtained in step (3), and concentrate the filtrate to 100 g to obtain a Chinese medicine composition.

[0101] Comparative Example 15

[0102] The only difference from Example 1 is that an equal amount of Radix Pseudostellariae is replaced with Leonurus japonicus, and the remaining raw materials and preparation method are the same as Example 1.

[0103] Comparative Example 16

[0104] The only difference from Example 1 is that an equal amount of Vaccaria segetalis is replaced by Polygonatum odoratum, and the remaining raw materials and preparation method are the same as Example 1.

[0105] Comparative Example 17

[0106] The only difference from Example 1 is that an equal amount of Rubia cordifolia is replaced with Ophiopogon japonicus, and the remaining raw materials and preparation method are the same as Example 1.

[0107] Test Example 1 Active ingredient content test

[0108] This test measures the contents of Pseudostellariae Radix Cyclic Peptide B and Vaccariae Semen Cyclic Peptide B in the samples prepared in Examples 1 and 3, Comparative Examples 1 and 2, and Comparative Examples 13 and 14.

[0109] 1.1 Determination of Pseudostellariae Cyclopeptide B Content - HPLC

[0110] Chromatographic column: C18 chromatographic column (4.6mm*250mm, 5μm), column temperature: 35°C, detection wavelength: 203nm, flow rate: 1.0mL / min; injection volume: 10μL; mobile phase A: water; mobile phase B: acetonitrile; gradient elution conditions are shown in Table 1.

[0111] Table 1

[0112]

[0113]

[0114] 1.2 Determination of Vaccaria cyclopeptide B content - HPLC

[0115] Chromatographic column: C18 chromatographic column (4.6mm*250mm, 5μm), column temperature: 40℃, detection wavelength: 220nm, flow rate: 1.0mL / min; injection volume: 10μL; mobile phase A: water; mobile phase B: acetonitrile; gradient elution conditions are shown in Table 2.

[0116] Table 2

[0117] Time (min) Mobile phase A (%) Mobile phase B (%) 0 68 32 7 68 32 15 40 60 20 30 70 22 68 32

[0118] The above test results are shown in Table 3.

[0119] Table 3

[0120] sample Pseudostellariae Cyclotide B (mg / kg) Vaccaria cyclopeptide B (mg / kg) Example 1 30.54 19.21 Example 3 56.34 11.12 Comparative Example 1 28.76 0 Comparative Example 2 0 19.04 Comparative Example 13 22.47 15.25 Comparative Example 14 17.18 11.41

[0121] From the results in Table 3, it can be seen that compared with Comparative Example 13, Example 1 and Comparative Example 1 have higher cyclopeptide contents due to homogenization during the preparation process, indicating that homogenization can increase the extraction content of cyclopeptides. It is possible that the plant tissue fiber structure is sheared during the homogenization process, so that the active substances can be better released into the solvent. Compared with Comparative Example 14, Example 1 and Comparative Example 2 can obtain more cyclopeptides, indicating that adjusting the extraction order and conditions of Vaccaria segetalis will affect the content of cyclopeptides. It may be that Vaccaria segetalis contains a large amount of starch, and the heating extraction causes the starch particles to begin to absorb water and swell, and the suspension becomes a translucent viscous colloidal solution. This viscous colloid wraps the active substance therein, which is retained in the filter residue during filtration, resulting in a low content of active substances in the extract.

[0122] Test Example 2 Antioxidant Efficacy Test

[0123] This test is to detect the DPPH scavenging ability and total antioxidant activity of the samples prepared in Examples 1-3, Comparative Examples 1-12 and 15-17.

[0124] Oxidative stress is a state in which the free radicals produced in the body exceed the body's ability to clear them with antioxidants, causing oxidative damage to cells and tissues. Excessive free radicals in the body can trigger oxidative stress, causing illness or aging. To defend against oxidative stress, we mainly rely on the body's antioxidant system.

[0125] 2.1 Evaluation of DPPH free radical scavenging ability

[0126] The ability to scavenge DPPH free radicals reflects the antioxidant capacity of a substance to a certain extent. The greater the free radical scavenging rate, the stronger the antioxidant capacity and the stronger the anti-aging ability. Therefore, the anti-aging effect of a sample can be judged by studying its ability to scavenge DPPH free radicals.

[0127] The experimental method, the steps are as follows:

[0128] Add 200 μL of DPPH test solution with a mass concentration of 50 mg / L and a certain amount of the sample to be tested to a centrifuge tube, add water to the total volume to 300 μL, shake well, react in the dark for 30 minutes, then aspirate 200 μL to an ELISA plate, and use the ELISA plate to measure the absorbance at a wavelength of 517 nm, which is recorded as A1; add 200 μL of anhydrous ethanol and a corresponding volume of the sample to be tested to a centrifuge tube, add water to the total volume to 300 μL, and follow the other steps as A1, and the measured absorbance is recorded as A2; add 200 μL of DPPH test solution and 100 μL of water to a test tube, follow the other steps as A1, and the measured absorbance is recorded as A3. Calculate the scavenging rate (Y) of the test solution for DPPH free radicals according to the following formula (1);

[0129] The calculation formula of DPPH free radical scavenging rate is: Y (%) = [1-(A1-A2) / A3] × 100% (1);

[0130] Where A1 is the absorbance of the system after the sample removes DPPH, A2 is the absorbance of the sample blank system, and A3 is the absorbance of the DPPH reaction system without adding the sample.

[0131] IC50: The concentration of the sample when the DPPH clearance rate is 50%. 50 ). IC 50 The smaller the value, the stronger the sample's ability to scavenge DPPH free radicals.

[0132] 2.2 FRAP method to determine total antioxidant activity

[0133] The experimental method, the steps are as follows:

[0134] 1) Sample determination: Add 100 μL of sample solution (dilute appropriately according to the situation) to the reaction tube, then add 3 mL of FRAP working solution, mix well, react at 37°C for 15 min, and measure the absorbance at 593 nm. Repeat each experiment three times;

[0135] 2) FeSO 4 Determination of standard curve: According to step 1), 0.1-2.0mmol / L FeSO 4 The standard solution is used instead of the sample to draw the standard curve;

[0136] 3) Calculation results: The final total antioxidant activity of the sample is expressed as FeSO 4 The equivalent concentration is expressed in mmol / L, as FeSO 4 is the standard solution. According to the A value after the reaction, the corresponding FeSO 4The concentration (mmol / L) is defined as the FRAP value. The larger the FRAP value, the stronger the antioxidant activity.

[0137] The above test results are shown in Table 4.

[0138] Table 4

[0139]

[0140]

[0141] From the results in Table 4, it can be seen that the Chinese medicine composition of the present invention has a certain scavenging effect on DPPH. 50 The value is lower than that of Example 1-2, indicating that less sample is needed to remove 50% of DPPH free radicals, and the removal effect is better. It also indicates that the amount of Chinese medicine raw materials affects the efficacy of the final composition; the IC 50 The values ​​are all lower than those of Comparative Examples 1-12, indicating that the antioxidant capacity of the Chinese medicine composition provided by the present invention is higher than that of a single medicinal material extract and a combination of other 2 or 3 medicinal materials, verifying the rationality of the prescription compatibility of the present invention; compared with Comparative Examples 1-12, the Chinese medicine composition in Examples 1-3 has better iron ion reduction ability. Comparative Examples 15-17 are all worse than Example 1 because the Chinese medicine raw materials of the present invention are replaced with other Chinese medicines, indicating that the present invention uses a combination of Pseudostellariae Radix, Vaccariae Semen, Rubia cordifolia and Aster tataricus to have a certain synergistic effect, thereby improving the antioxidant effect.

[0142] Test Example 3 Anti-inflammatory efficacy test

[0143] This test is conducted on samples prepared in Examples 1 and 3, Comparative Examples 1-4, 8-10, 12 and 15-17 to detect the contents of IL-6 and TNF-α.

[0144] Inflammation is part of the body's defense mechanism. It occurs when the immune system recognizes and eliminates internal or external harmful stimuli and begins to heal. Aging is a common, complex, and naturally occurring process. The concept of inflammatory aging is derived from immune aging. When the immune system ages, it cannot remove senescent cells and proinflammatory factors, and the level of inflammation will increase accordingly. When low-level chronic inflammation gradually accumulates, it will further aggravate aging and form a vicious cycle. Macrophages can promote and mediate inflammation. High concentrations of chemokines at the site of inflammation can interact with corresponding receptors on the surface of macrophages, induce cell activation, and recruit them to the site of infection. Activated macrophages can release various proinflammatory factors such as IL-6 and TNF-α.

[0145] The experimental method includes the following steps:

[0146] After filtering the samples with a 0.2 μm sterile filter, they were diluted with DMEM complete culture medium to obtain sample solutions of different concentrations required for the experiment.

[0147] RAW264.7 cells were cultured in DMEM medium containing 10% FBS at a rate of 6 × 10 cells per well. 4 The cells were seeded at a density of 100 / well in a 24-well plate, with 1 mL of complete culture medium in each well. After 12 hours, a medium containing low-concentration (2%) serum and LPS were added, and then samples of various concentrations were added. After 24 hours of culture, the supernatant was collected. The IL-6 and TNF-α contents were detected according to the instructions of the kit.

[0148] The production of IL-6 and TNF-α in the sample group and the model group was normalized to obtain the inhibition rate of IL-6 and TNF-α content in the sample group compared with the model group. The test results are shown in Table 5.

[0149] Table 5

[0150] Group (0.1% mass fraction) IL-6 (%) TNF-α(%) Example 1 51.42 41.36 Example 3 60.86 48.27 Comparative Example 1 25.52 18.26 Comparative Example 2 20.12 22.21 Comparative Example 3 37.24 31.25 Comparative Example 4 33.57 34.83 Comparative Example 8 42.31 39.69 Comparative Example 9 41.85 37.65 Comparative Example 10 40.54 38.76 Comparative Example 12 35.43 32.23 Comparative Example 15 40.52 37.56 Comparative Example 16 36.86 34.89 Comparative Example 17 30.46 28.62

[0151] As shown in Table 5, compared with Ratio 1-4, the anti-inflammatory effect of the Chinese medicine composition provided in the embodiment of the present invention is better than that of a single medicinal material extract, which verifies the rationality of the prescription compatibility of the present invention. As can be seen from Comparative Examples 1-4, Pseudostellariae Radix Extract, Vaccariae Semen Extract, Rubia Cordata Extract and Aster Extract all have certain anti-inflammatory effects, and Rubia Cordata and Aster Extract have more obvious inhibitory effects on inflammatory factors IL-6 and TNF-α, wherein Rubia Cordata Extract has more obvious inhibition on inflammatory factor IL-6, and Aster Extract has better inhibition on inflammatory factor TNF-α. Comparative Examples 15-17 are all worse than Example 1 because the Chinese medicine raw materials of the present invention are replaced with other Chinese medicines, indicating that the present invention adopts Pseudostellariae Radix, Vaccariae Semen, Rubia Cordata and Aster Compound to have certain synergistic effects, thereby improving the anti-inflammatory effect.

[0152] Test Example 4

[0153] This test is for samples prepared in Examples 1 and 3, Comparative Examples 1-4, 6, 8 and 15-17, and the content of type I collagen (COL-1) and elastin is determined.

[0154] Type I collagen (COL-1) is mainly found in the skin, bones, muscles and other connective tissues, playing a key role in maintaining the integrity and elasticity of tissue structures. When COL-1 is specifically degraded, the normal structure of collagen fibers and elastic fibers in the body is destroyed, which can lead to aging phenomena such as wrinkles on the skin. Elastin is the main component of elastic fibers. Although elastin only accounts for 2% of the total protein in the dermis, it plays an important role in skin elasticity. Elastin is the main cause of loose, sagging and fine wrinkles in aging skin.

[0155] The experimental method includes the following steps:

[0156] After filtering the samples with a 0.2 μm sterile filter, they were diluted with DMEM complete culture medium to obtain sample solutions of different concentrations required for the experiment.

[0157] HSF human fibroblasts were cultured at 1×10 4 The cells were seeded at a density of 1.50 μg / ml in a 96-well plate and incubated at 37°C with 5% CO 2 After culturing in an incubator for 48 h, discard the old culture medium. Add 100 μL of cell culture medium to the wells containing cells. After incubation for 1 h, add 100 μL of 300 μM H 2 O 2 Complete medium (DMEM cell culture medium containing 10% serum) was added to each well containing cells as a negative control group. 100 μL of the sample to be tested was added to each well containing cells. After incubation for 1 hour, 100 μL of 300 μM H 2 O 2 100 μL of complete medium was added to the wells without cells, and after incubation for 1 h, 100 μL of 300 μM HCl was added to each well. 2 O 2 Complete medium of 100 μL was added to the wells containing cells as the blank control group. After incubation for 1 h, 100 μL of complete medium containing 300 μM H 2 O 2 The complete medium was used as the modeling group. Each group was set up with 3 replicates. 2 After culturing in a 37°C incubator for 24 hours, the cell supernatant was taken and the production of type I collagen (COL-1) and elastin in each group was determined using a kit. The steps were strictly carried out according to the instructions. The production of type I collagen and elastin in the sample group and the modeling group was normalized to obtain the increase rate of type I collagen and elastin in the sample group compared with the modeling group. The test results are shown in Table 6.

[0158] Table 6

[0159] Group (0.1% mass fraction) Type I collagen enhancement rate (%) Elastin enhancement rate (%) Example 1 55.85 41.36 Example 3 63.21 45.27 Comparative Example 1 43.28 35.21 Comparative Example 2 36.25 30.57 Comparative Example 3 25.47 23.45 Comparative Example 4 22.34 18.57 Comparative Example 6 49.54 37.41 Comparative Example 8 44.25 35.75 Comparative Example 15 35.37 29.01 Comparative Example 16 26.51 24.23 Comparative Example 17 21.54 19.81

[0160] As shown in Table 6, compared with Ratios 1-4, the effect of the Chinese medicine composition provided in the embodiment of the present invention on promoting type I collagen and elastin is better than that of a single medicinal material extract, which verifies the rationality of the composition and compatibility of the present invention. As can be seen from Comparative Examples 1-4, the extracts of Radix Pseudostellariae and Semen Vaccariae have a more obvious effect on promoting the production of type I collagen and elastin. Comparative Examples 15-17 are all worse than Example 1 because the Chinese medicine raw materials of the present invention are replaced with other Chinese medicines, indicating that the present invention uses Radix Pseudostellariae, Semen Vaccariae, Radix Rubiae and Aster to have a certain synergistic effect, thereby enhancing the effect of promoting the production of type I collagen and elastin.

[0161] Application Example 1

[0162] An essence cream includes the Chinese medicine composition prepared in Example 3 of the present invention. The specific formula is shown in Table 7; and the preparation method thereof can be prepared according to the conventional preparation method of essence cream.

[0163] Table 7

[0164] Element Mass fraction (%) water margin Jojoba (SIMMONDSIA CHINENSIS) seed oil 5 Caprylic / capric triglyceride 6 Ammonium Acryloyldimethyltaurate / VP Copolymer 3 Propyl heptyl octanoate 3 Example 3 Chinese medicine composition prepared 1 Lecithin 0.3 Sodium Hyaluronate 0.050 glycerin 5.00 Sodium Stearoyl Glutamate 0.2 1,3-Butanediol 2.00 1,2-Hexanediol 1.0 p-Hydroxyacetophenone 0.2

[0165] Comparative application example 1

[0166] The essence cream of comparative application example 1 is different from application example 1 only in that an equal amount of the Chinese medicine composition prepared in example 3 is replaced with water, and other ingredients, addition amounts and preparation methods are the same as those of application example 1.

[0167] Test Example 5: Essence Cream Efficacy Test

[0168] The human efficacy of the essence creams of Application Example 1 and Comparative Application Example 1 was evaluated.

[0169] 5.1 Human moisturizing experiment

[0170] 30 volunteers aged 20-45 were selected. The essence cream of Application Example 1 and Comparative Application Example 1 were used on the left and right faces respectively. The essence cream was used twice a day, once in the morning and once in the evening, for 28 consecutive days. The subjects were followed up 14 days and 28 days after using the product, and the facial skin water content (au) was tested with a skin moisture tester Corneometer to comprehensively evaluate the moisturizing effect of the product on human skin.

[0171] 5.2 Skin elasticity test

[0172] The skin elasticity tester MPA580 (CourageKhazaka, Germany) was used. The instrument sucked the skin into a specific probe under negative pressure, and the depth of the sucked skin was measured by a non-contact optical test system. The elastic properties of the skin were analyzed using MPA software. The ratio of the amount of skin rebound without negative pressure to the maximum amount of skin stretching with negative pressure was used to evaluate skin elasticity. The closer the ratio is to 1, the better the skin elasticity. 30 volunteers aged 20-45 years were selected. The subjects used the product on their faces once a day in the morning and evening. Before use (D0), on the 14th day of use (D14), and on the 28th day of use (D28), at a temperature of 21±1°C and a humidity of 50±10%, the subjects cleaned their faces and waited for 20 minutes before measuring the facial elasticity F4 value and elasticity R2 value, and comprehensively evaluated the firming effect of the product on human skin. The smaller the F4 value, the stronger the skin firmness per unit time and per unit cross-sectional area. The closer the R2 value is to 1, the better the skin elasticity per unit time and per unit cross-sectional area.

[0173] The results are shown in Table 8.

[0174] Table 8

[0175]

[0176] As shown in Table 8, compared with the product of comparative application example 1, the product of application example 1 can significantly improve the water content and elasticity of the skin due to the Chinese medicine composition of the present invention. Compared with the sample before use, the product of application example 1 has a skin elasticity (F4) of 4.94% lower, skin elasticity (R2) of 4.57% higher, and skin water content of 8.17% higher after 14 days of use; after 28 days of use, skin elasticity (F4) of 8.79% lower, skin elasticity (R2) of 7.57% higher, and skin water content of 18.60% higher, indicating that the Chinese medicine composition of the present invention is added to cosmetics, which can significantly improve the water content of the stratum corneum of the skin, effectively reduce the amount of transepidermal water loss, effectively improve skin elasticity, and have a firming effect.

[0177] The preferred embodiments of the present invention are specifically described above, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A Chinese medicine composition, characterized in that: In terms of weight, the Chinese medicinal raw materials used in the Chinese medicinal composition include 10-60 parts of Pseudostellaria heterophylla, 10-40 parts of Vaccaria segetalis, 10-40 parts of Rubia cordifolia and 10-40 parts of Aster tataricus.

2. The Chinese medicine composition according to claim 1, characterized in that: In terms of weight, the Chinese medicinal raw materials used in the Chinese medicinal composition include 20-50 parts of Pseudostellaria heterophylla, 10-30 parts of Vaccaria segetalis, 10-30 parts of Rubia cordifolia and 10-30 parts of Aster tataricus.

3. A method for preparing a Chinese medicine composition, characterized in that: The following steps are involved: S1, mixing Pseudostellaria heterophylla, Rubia cordifolia, Aster tataricus and a solvent, and homogenizing to obtain a mixed solution; S2, heating the mixed solution to extract and obtain an extract; S3. Mix the extract with Vaccaria segetalis, homogenize, filter and concentrate to obtain a Chinese medicine composition.

4. The preparation method according to claim 3, characterized in that: The solvent includes water or a polyol.

5. The preparation method according to claim 3, characterized in that: In the step S1, the homogenization speed is 1000-8000 rpm, and the homogenization time is 10-60 min.

6. The preparation method according to claim 3, characterized in that: In the step S3, the homogenization speed is 1000-8000 rpm, and the homogenization time is 20-90 min.

7. The preparation method according to claim 3, characterized in that: The temperature of the heating extraction is 50-100° C.; and / or, the number of the heating extraction is 2-3 times; and / or, the time of the heating extraction is 1-3 hours.

8. A cosmetic, characterized in that: The cosmetic comprises the Chinese medicine composition according to claim 1 or 2, or the Chinese medicine composition prepared by the preparation method according to any one of claims 3-7.

9. The cosmetic according to claim 8, characterized in that: The content of the traditional Chinese medicine composition is 0.01wt-10wt%.

10. The cosmetic according to claim 8, characterized in that: The dosage form of the cosmetic includes any one of an aqueous solution, an emulsion, a spray, a cream, an essence or a facial mask.

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