An anti-aging composition and its preparation method and application

Abstract

The invention discloses an anti-aging composition and its preparation method and application, and relates to the field of cosmetic technology. The invention provides an anti-aging composition, including the following components in parts by weight: 1-10 parts of supercritical peony fermented seed oil and 0.1-1 parts of oil-soluble peony callus extract; the preparation method of the supercritical peony fermented seed oil, comprising the steps of: adding the activated plant lactobacillus liquid to supercritical peony seed oil for fermentation under high pressure and high oxygen conditions, and centrifugation to obtain supercritical peony fermented seed oil; the high pressure condition is 20-24mpa, and the high oxygen condition is to add a fluorinated liquid containing saturated high-purity oxygen with a volume concentration of 15-18%. The present invention ferments supercritical peony seed oil under specific high pressure and high oxygen conditions by two different plant lactobacilli, and the obtained supercritical peony fermented seed oil can be better fused with oil-soluble peony callus, and no oil particles appear.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to an anti-aging composition and a preparation method and application thereof. Background Art

[0002] As people's living standards continue to improve and their pursuit of health and beauty increases, the demand for natural, safe and efficient skin care products is becoming more and more urgent. As an emerging high-end vegetable oil, peony seed oil has attracted much attention in recent years. Peony seed oil is rich in unsaturated fatty acids such as linolenic acid, which has many important physiological functions such as regulating blood lipids, anti-oxidation, and delaying aging. Linolenic acid plays an irreplaceable role in maintaining normal physiological metabolism of the human body and preventing and improving a variety of chronic diseases. However, at present, there are still certain limitations in the application of pure peony seed oil, and its efficacy needs to be further improved.

[0003] Peony callus extract is a natural active ingredient extracted from peony callus, which contains rich bioactive ingredients such as flavonoids, phenols, polysaccharides, and has multiple effects such as anti-inflammatory, antibacterial, antioxidant, and cell regeneration. These active ingredients have a synergistic effect with the unsaturated fatty acids in peony seed oil, which can further enhance the skin care effect of the product. In the prior art, although peony seed oil and peony extract each have certain applications in different fields, the process optimization treatment of high linolenic peony seed oil and peony callus extract is scientifically and rationally matched to give full play to the synergistic advantages of the two, which is rarely reported at present.

[0004] Therefore, an anti-aging composition with a stable system, good anti-wrinkle effect, and the ability to effectively inhibit tyrosinase activity and inhibit the production of melanin has broad application prospects. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-aging composition with a stable system, good anti-wrinkle effect, and the ability to effectively inhibit tyrosinase activity and inhibit the production of melanin, which has broad application prospects.

[0006] To achieve the above purpose, the technical scheme adopted by the present invention is: an anti-aging composition, comprising the following components in parts by weight: 1-10 parts of supercritical peony fermented seed oil and 0.1-1 parts of oil-soluble peony callus extract; the preparation method of the supercritical peony fermented seed oil comprises the following steps: adding activated plant lactobacillus bacterial liquid to supercritical peony seed oil under high pressure and high oxygen conditions for fermentation, and centrifugation to obtain the supercritical peony fermented seed oil; the high pressure condition is 20-24 MPa, and the high oxygen condition is adding fluoride liquid containing saturated high-purity oxygen with a volume concentration of 15-18%; the plant lactobacillus bacterial liquid is a mixture of two different plant lactobacillus bacterial liquids.

[0007] Preferably, the Lactobacillus plantarum liquid is a mixture of Lactobacillus plantarum liquid A and Lactobacillus plantarum liquid B.

[0008] The plant lactobacillus liquid A has a rich and diverse enzyme system. In addition to common lipases, it can also produce some special enzymes, which can decompose and transform different components in vegetable oil more comprehensively and deeply, increase the diversity of fermentation products; can produce unique fermentation aroma and flavor substances, so that the fermented vegetable oil has a richer and more complex flavor, and enhance its application value in the fields of food. Plant lactobacillus liquid B can efficiently utilize substrates, quickly utilize specific components in vegetable oil as carbon sources and energy sources, quickly start the fermentation process, and shorten the fermentation cycle; and has a strong decomposition ability for certain complex components in vegetable oil, which can be converted into simple substances that are more easily utilized by subsequent microorganisms, laying the foundation for the entire fermentation process; can produce a large amount of organic acids, such as lactic acid, etc. during the fermentation process, so that the pH value of the fermentation environment is reduced. This acidic environment is conducive to inhibiting the growth of miscellaneous bacteria and ensuring the purity of the fermentation.

[0009] Preferably, the volume ratio of the fluorinated liquid containing saturated high-purity oxygen at a volume concentration of 15-18% to the supercritical peony seed oil is (0.5-0.8):(1-1.2).

[0010] Preferably, the volume ratio of the Lactobacillus plantarum liquid A to the Lactobacillus plantarum liquid B is (1-3):(1-3); further preferably, the volume ratio of the Lactobacillus plantarum liquid A to the Lactobacillus plantarum liquid B is 1:1.

[0011] The plant lactobacillus A is preserved in Beijing Technology and Business University, its Latin name is Lactiplantibacillusplantarum, and its preservation number is NMGL2; the plant lactobacillus B is preserved in China Industrial Microbiological Culture Collection Center, its Latin name is L.plantanum, and its preservation number is CICC 20022, and it was purchased from China Industrial Microbiological Culture Collection Center.

[0012] The oil-soluble peony callus extract is rich in a variety of antioxidant ingredients, such as flavonoids and phenolic compounds. These ingredients have the following effects: (1) They can effectively remove excessive free radicals in the body and reduce the oxidative damage of free radicals to cells; (2) They can activate the signaling pathways of collagen synthesis, such as the transforming growth factor-β (TGF-β) pathway, increase the elasticity and firmness of the skin, and reduce the formation of wrinkles; (3) They can inhibit the activity of MMPs, reduce the degradation of collagen, and maintain the collagen content of the skin; (4) They can protect cell DNA from the influence of these damaging factors, reduce DNA mutation and damage, accelerate the DNA repair process, and maintain the normal function of cells. The content of unsaturated fatty acids in peony seed oil is as high as 92% or more, among which the content of linoleic acid and α-linolenic acid is relatively high, and the content of α-linolenic acid is as high as 40% or more. It is known as "liquid gold" and has the following effects: (1) Linoleic acid can not only promote the synthesis of lipids between skin cells, enhance the barrier function of the skin, and prevent water evaporation, but also be converted into prostaglandin E3 with anti-inflammatory effect, reduce the release of inflammatory mediators, relieve skin redness, itching and other symptoms, and even effectively promote the production of collagen, reduce the formation of wrinkles, prevent light damage, promote the repair and regeneration of skin cells, and reduce the adverse effects of ultraviolet rays on the skin. (2) Peony seed oil can increase its linolenic acid content after supercritical extraction. The linolenic acid content of supercritically extracted peony seed oil is more than 60%.

[0013] However, it is found in actual research that when supercritical peony seed oil with high linolenic acid is mixed with oil-soluble peony callus, transparent oil particles will appear suspended therein, and the two cannot be completely integrated, which may be caused by the large molecular weight of sterols contained in the supercritical peony seed oil. The present invention ferments supercritical peony seed oil under specific high pressure and high oxygen conditions by plant lactobacillus, and the obtained supercritical peony fermented seed oil can be better fused with oil-soluble peony callus without the appearance of oil particles.

[0014] In actual research, it was found that the double plant lactobacillus has the following advantages: ① It can synergistically improve the ability to decompose oil. When the two plant lactobacilli are used together, their enzyme systems can complement each other and decompose and transform various components in vegetable oil more comprehensively and efficiently; ② The double plant lactobacilli can play their respective advantages at different stages. In the early stage, one strain quickly starts fermentation to create a suitable fermentation environment for the subsequent another strain; in the later stage, the other strain can further deepen the fermentation and improve the quality and yield of the product; ③ Improve the ability to adapt to environmental changes and reduce the risk of fermentation failure.

[0015] The inventors used high performance liquid chromatography to determine that the linolenic acid content of supercritical peony fermented seed oil was above 60%, and the linolenic acid content was not much different from that of unfermented supercritical peony seed oil. Therefore, fermentation did not affect the linolenic acid content.

[0016] Preferably, the anti-aging composition comprises the following components in parts by weight: 5-8 parts of supercritical fermented peony seed oil and 0.3-0.5 parts of oil-soluble peony callus extract.

[0017] Preferably, the method for activating Lactobacillus plantarum is: adding Lactobacillus plantarum to MRS culture medium for activation culture, the culture temperature is 25-37° C., and the culture time is 24-48 hours.

[0018] Preferably, the volume ratio of the Lactobacillus plantarum liquid to the supercritical peony seed oil is (5-10):100.

[0019] Preferably, the fermentation temperature is 23-28°C and the fermentation time is 24-48h.

[0020] In addition, the present invention provides a method for preparing the anti-aging composition, comprising the following steps: mixing supercritical peony fermented seed oil and oil-soluble peony callus to obtain the anti-aging composition.

[0021] Preferably, the mixing is performed by ultrasonic intermittent treatment, with ultrasonic treatment for 5-10 minutes, pause for 10-15 minutes, and treatment for 2-3 times to ensure that the mixture is fully mixed.

[0022] Preferably, the ultrasonic wave has a frequency of 20-40 kHz and a power of 200-300 W.

[0023] Furthermore, the present invention provides the use of the anti-aging composition in the preparation of skin products.

[0024] The present invention provides a skin product, which comprises the anti-aging composition.

[0025] Preferably, the skin product comprises one of lotion, emulsion, cream, facial mask, essence and spray.

[0026] Optionally, the present invention provides a cream comprising the following components in percentage by mass: 1-10% anti-aging composition, 6-15% moisturizer, 0.2-0.5% thickener, 0.16-0.5% pH adjuster, 1-5% emulsifier, 0.5-2% preservative, and the balance deionized water.

[0027] Exemplarily, the moisturizer includes at least one of glycerol, butylene glycol, and 1,3-propylene glycol; the thickener includes at least one of carbomer, xanthan gum, and polyacrylic acid; the emulsifier includes at least one of lecithin and polyglycerol emulsifiers; the preservative includes at least one of 1,2-hexanediol, 1,2-pentanediol, and sodium benzoate; the pH adjuster includes at least one of arginine, tromethamine, and triethanolamine.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention ferments supercritical peony seed oil under specific high-pressure and high-oxygen conditions by using Lactobacillus biplantarum, and the obtained supercritical peony fermented seed oil can be better fused with the oil-soluble peony callus without the appearance of oil particles. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The anti-aging composition prepared in Example 1 is shown after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days;

[0030] Figure 2 The anti-aging composition prepared in Comparative Example 3 is shown after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days;

[0031] Figure 3 These are pictures of the anti-aging composition prepared in Comparative Example 6 after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days. DETAILED DESCRIPTION

[0032] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0033] The raw materials of the present invention are now further described, but are not limited to the following raw materials:

[0034] Oil-soluble peony callus extract: Weiming Shiguang Biotechnology Co., Ltd.

[0035] Supercritical peony seed oil: Weiming Shiguang Biotechnology Co., Ltd.

[0036] Lactobacillus plantarum A: deposited in Beijing Technology and Business University, Latin name: Lactiplantibacillus plantarum, deposit number: NMGL2, purchased from the Beijing Technology and Business University Collection Center;

[0037] Lactobacillus plantarum B: deposited in China Industrial Microorganism Culture Collection Administration Center, Latin name: L. plantanum, deposit number: CICC 20022, purchased from China Industrial Microorganism Culture Collection Administration Center.

[0038] Candida: preserved in the Fermented Glycerol Research and Design Center of Jiangnan University, Latin name: Candida glvcerinogenes, deposit number: WL2002-5, purchased from the Fermented Glycerol Research and Design Center of Jiangnan University.

[0039] Supercritical fermented peony seed oil-1

[0040] The preparation method of supercritical peony fermented seed oil-1 comprises the following steps:

[0041] (1) 10^8-10^9 CFU / mL of Lactobacillus plantarum A and Lactobacillus plantarum B were added to MRS medium for activation culture at 25°C for 48 h.

[0042] (2) adding the activated Lactobacillus plantarum bacterial liquid to supercritical peony seed oil for fermentation under high pressure and high oxygen conditions, wherein the fermentation temperature is 28° C., the fermentation time is 24 h, the volume ratio of the Lactobacillus plantarum bacterial liquid to the supercritical peony seed oil is 5:100, and centrifugal filtration is performed to obtain supercritical peony fermented seed oil; the high pressure condition is 20 MPa, the high oxygen condition is to add a fluorinated liquid containing 18% volume concentration of saturated high-purity oxygen, and the volume ratio of the fluorinated liquid containing 18% volume concentration of saturated high-purity oxygen to the supercritical peony seed oil is 0.8:1; the Lactobacillus plantarum bacterial liquid is a mixture of Lactobacillus plantarum bacterial liquid A and Lactobacillus plantarum bacterial liquid B, and the volume ratio of Lactobacillus plantarum bacterial liquid A to Lactobacillus plantarum bacterial liquid B is 1:1.

[0043] Supercritical fermented peony seed oil-2

[0044] The preparation method of supercritical peony fermented seed oil-2 comprises the following steps:

[0045] (1) 10^8-10^9 CFU / mL of Lactobacillus plantarum A and Lactobacillus plantarum B were added to MRS medium for activation culture at 37°C for 24 h;

[0046] (2) adding the activated Lactobacillus plantarum bacterial liquid to supercritical peony seed oil for fermentation under high pressure and high oxygen conditions, wherein the fermentation temperature is 23° C., the fermentation time is 48 hours, the volume ratio of the Lactobacillus plantarum bacterial liquid to the supercritical peony seed oil is 10:100, and centrifugal filtration is performed to obtain supercritical peony fermented seed oil; the high pressure condition is 24 MPa, the high oxygen condition is to add a fluorinated liquid containing 15% volume concentration of saturated high-purity oxygen, and the volume ratio of the fluorinated liquid containing 15% volume concentration of saturated high-purity oxygen to the supercritical peony seed oil is 0.8:1; the Lactobacillus plantarum bacterial liquid is a mixture of Lactobacillus plantarum bacterial liquid A and Lactobacillus plantarum bacterial liquid B, and the volume ratio of Lactobacillus plantarum bacterial liquid A to Lactobacillus plantarum bacterial liquid B is 1:1.

[0047] Supercritical fermented peony seed oil-3

[0048] The preparation method of the supercritical peony fermented seed oil-3 is different from that of the supercritical peony fermented seed oil-1, except that the high pressure condition is 28 MPa, and the other components, proportions and preparation methods are exactly the same.

[0049] Supercritical fermented peony seed oil-4

[0050] The preparation method of the supercritical peony fermented seed oil-4 is different from that of the supercritical peony fermented seed oil-1 in that the only difference is the high oxygen condition, which is a fluorinated liquid with saturated high-purity oxygen at a volume concentration of 25%, and the other components, proportions and preparation methods are exactly the same.

[0051] Supercritical fermented peony seed oil-5

[0052] The preparation method of the supercritical peony fermented seed oil-5 is different from that of the supercritical peony fermented seed oil-1, except that the fermentation bacteria are replaced by Lactobacillus plantarum B, and the other components, proportions and preparation methods are exactly the same.

[0053] Supercritical fermented peony seed oil-6

[0054] Compared with supercritical peony fermented seed oil-1, the preparation method of the supercritical peony fermented seed oil-6 is different only in the fermentation strains, that is, the double plant lactobacillus liquid is replaced by a mixed liquid of plant lactobacillus B and Candida albicans in a volume ratio of 1:1, and the other components, proportions and preparation methods are exactly the same.

[0055] Examples and Comparative Examples

[0056] Example 1

[0057] An anti-aging composition comprises the following components in parts by weight: 1.5 parts of supercritical fermented peony seed oil and 1.05 parts of oil-soluble peony callus extract;

[0058] The preparation method of the anti-aging composition comprises the following steps:

[0059] Supercritical fermented peony seed oil and oil-soluble peony callus are mixed, and the mixing is subjected to ultrasonic-assisted intermittent treatment, with ultrasonic treatment for 8 minutes and pause for 10 minutes, and treatment is performed twice. The ultrasonic frequency is 40 kHz and the power is 200 W to obtain the anti-aging composition.

[0060] Example 2

[0061] Compared with Example 1, the only difference is that the weight parts of the anti-aging composition are selected differently, including the following components in weight parts: 8 parts of supercritical fermented peony seed oil and 0.3 parts of oil-soluble peony callus extract, and the other components, proportions and preparation methods are exactly the same.

[0062] Example 3

[0063] Compared with Example 1, the only difference is that the weight parts of the anti-aging composition are different, including the following components in weight parts: 1 part of supercritical fermented peony seed oil and 0.1 part of oil-soluble peony callus extract, and the other components, proportions and preparation methods are exactly the same.

[0064] Example 4

[0065] Compared with Example 1, the only difference is that the weight parts of the anti-aging composition are selected differently, including the following components in weight parts: 10 parts of supercritical fermented peony seed oil-1 and 0.8 parts of oil-soluble peony callus extract-1, and the other components, proportions and preparation methods are exactly the same.

[0066] Example 5

[0067] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and supercritical fermented peony seed oil-2 is selected, and the other components, proportions and preparation methods are exactly the same.

[0068] Comparative Example 1

[0069] Compared with Example 1, the only difference is that the weight parts of the anti-aging composition are selected differently, including the following components in weight parts: 15 parts of supercritical fermented peony seed oil and 0.05 parts of oil-soluble peony callus extract, and the other components, proportions and preparation methods are exactly the same.

[0070] Comparative Example 2

[0071] Compared with Example 1, the only difference is that the weight parts of the anti-aging composition are different, including the following components in weight parts: 0.5 parts of supercritical fermented peony seed oil and 1 part of oil-soluble peony callus extract, and the other components, proportions and preparation methods are exactly the same.

[0072] Comparative Example 3

[0073] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and supercritical fermented peony seed oil-3 is selected.

[0074] Comparative Example 4

[0075] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and supercritical fermented peony seed oil-4 is selected.

[0076] Comparative Example 5

[0077] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and supercritical fermented peony seed oil-5 is selected.

[0078] Comparative Example 6

[0079] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and supercritical fermented peony seed oil-6 is selected.

[0080] Comparative Example 7

[0081] Compared with Example 1, the only difference is that the supercritical fermented peony seed oil in the anti-aging composition is different, and unfermented supercritical peony seed oil is selected.

[0082] Application Examples

[0083] Application Examples 1-6 of the present invention, Comparative Application Examples 1-7 and Blank Application Examples provide a cream, the components (mass percentage) of the cream are shown in Table 1;

[0084] The anti-aging compositions used in Application Examples 1-5 are the anti-aging compositions prepared in Examples 1-5, respectively, and the anti-aging compositions used in Comparative Application Examples 1-7 are the anti-aging compositions prepared in Comparative Examples 1-7, respectively; for example, the anti-aging composition used in Application Example 1 is the anti-aging composition prepared in Example 1, the anti-aging composition used in Application Example 2 is the anti-aging composition prepared in Example 2, the anti-aging composition used in Comparative Application Example 1 is the anti-aging composition prepared in Comparative Example 1, and the same can be said for other examples.

[0085] The anti-aging composition used in Application Example 6 is the anti-aging composition prepared in Example 1.

[0086] The method for preparing the cream of the present invention comprises the following steps:

[0087] 1. Weigh the raw materials of phase A in sequence, homogenize them appropriately until there are no obvious large particles, stir and heat them to 75°C to make the material into a transparent material with a certain consistency, and keep it warm for 30 minutes;

[0088] 2. Mix phase B and heat it to 65°C to completely dissolve it; add the raw materials of phase B to phase A, homogenize for 4 minutes, and stir evenly;

[0089] 3. When the temperature drops to 45°C, add the C phase raw material, stir evenly, take the material for observation, and after all physical and chemical indicators are qualified, filter the material with a 200-mesh filter cloth.

[0090] Table 1

[0091]

[0092] Performance Test-1 Stability

[0093] The anti-aging compositions prepared in the examples and comparative examples were placed at 48°C±2°C, 25°C±2°C, 5°C±2°C and -18°C±2°C for 30 days, and the stability was recorded.

[0094] Stability test environment:

[0095] High temperature (48℃±2℃): Place the test sample in a sealed clean container, and then place it in a constant temperature incubator pre-adjusted to (48℃±2℃). Take a sample after 30 days, and observe the phenomenon after returning to room temperature;

[0096] Normal temperature (25℃±2℃): Place the test sample in a sealed clean container, and then place it in a constant temperature incubator pre-adjusted to (25℃±2℃). Take a sample after 30 days, and observe the phenomenon after returning to room temperature;

[0097] Cold resistance (5℃±2℃): Place the test sample in a sealed clean container, and then place it in a constant temperature refrigerator pre-adjusted to (5℃±2℃). Take a sample after 30 days, and observe the phenomenon after returning to room temperature;

[0098] Freeze resistance (-18℃±2℃): Place the test sample in a sealed clean container, and then place it in a constant temperature refrigerator pre-adjusted to (-18℃±2℃). Take a sample after 30 days and observe the phenomenon after returning to room temperature.

[0099] The stability results are divided into several levels: Level A: no oil particles and no stratification; Level B: trace oil particles and no stratification; Level C: a small amount of oil particles and no stratification; Level D: a large number of oil particles gathered together.

[0100] The experimental results are shown in Table 2 below.

[0101] Table 2

[0102] Group 48℃ 25℃ 5℃ -18℃ Example 1 A A A A Example 2 A A A A Example 3 A A A A Example 4 A A A A Example 5 A A A A Comparative Example 1 B B B C Comparative Example 2 B B B C Comparative Example 3 B C D D Comparative Example 4 B C D D Comparative Example 5 C C D D Comparative Example 6 B B C C Comparative Example 7 C C D D

[0103] Figure 1 The anti-aging composition prepared in Example 1 is shown after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days; Figure 2 The anti-aging composition prepared in Comparative Example 3 is shown after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days; Figure 3 These are pictures of the anti-aging composition prepared in Comparative Example 6 after being placed for 30 days; wherein, (a) is a picture of the anti-aging composition at 48°C±2°C for 30 days, (b) is a picture of the anti-aging composition at 25°C±2°C for 30 days, (c) is a picture of the anti-aging composition at 5°C±2°C for 30 days, and (d) is a picture of the anti-aging composition at -18°C±2°C for 30 days.

[0104] As can be seen from the table above, fermented supercritical peony seed oil can better fuse with oil-soluble peony callus without oil particles, and high pressure, high oxygen, and the selection of bacteria are also extremely important for the compatibility between the two. Although the stability of single plant lactobacillus and other bacteria is relatively acceptable, it is not as good as that of double plant lactobacillus. This shows that the enzymes produced by double plant lactobacillus fermentation can better promote the interaction between water and oil, oil and oil, hydrolyze triglycerides, reduce molecular weight, and make it easier to mix with oil-soluble peony callus.

[0105] Performance Test-2 Determination of Type I Collagen Content

[0106] Test method: 84 6-day-old zebrafish were divided into 14 groups and exposed to 5 g / L test sample solution (cream provided in Application Examples 1-6, Comparative Application Examples 1-7 and Blank Application Examples). A blank control group was also set up. After 24 hours of exposure, RNA was extracted from the zebrafish, cDNA was synthesized, and real-time PCR amplification was performed. β-actin was used as the housekeeping gene and Ct was used as the amplification result. The relative expression level of col1a1b was calculated and statistical analysis was performed.

[0107] col1a1b expression promotion rate % = [(gene expression level in the test group - gene expression level in the blank control group) / gene expression level in the blank control group] * 100%.

[0108] The test results are shown in Table 3.

[0109] Table 3

[0110] Group col1a1b expression promotion rate % Blank Application Example 12 Application Example 1 83 Application Example 2 79 Application Example 3 61 Application Example 4 66 Application Example 5 80 Application Example 6 63 Comparative application example 1 37 Comparative Application Example 2 43 Comparative Application Example 3 36 Comparative Application Example 4 42 Comparative Application Example 5 39 Comparative Application Example 6 44 Comparative Application Example 7 32

[0111] It can be seen from the above table that supercritical peony seed oil fermented by Lactobacillus plantarum can better be compatible with the oil-soluble peony callus extract and promote its efficacy. At the same time, the selection of bacterial strains and high-pressure and high-oxygen conditions are also crucial to the efficacy of the composition.

[0112] Performance Test-3 Senescent Cell Clearance Rate

[0113] Test method: set up experimental group, model group and control group for comparative experiment. Take cells in logarithmic growth period and press (8-10)x10 3 Each well was inoculated in a 96-well plate, incubated in an incubator at 37°C and 5% CO2 for 24 hours, the supernatant was discarded, and the plate was washed twice with PBS. The experimental samples (creams provided in Application Examples 1-6, Comparative Application Examples 1-7, and Blank Application Examples) were dissolved in fresh DMEM culture medium to prepare a 1% solution, 100uL per well, and incubated for 24 hours. After adding 10μL CCK-8 reagent and culturing for 2 hours, the OD value at 450nm was measured to calculate the clearance rate of senescent cell number. The clearance rate of senescent cells in the control group was 100%.

[0114] Experimental groups: model group - UVB stimulation first and then adding serum-free culture medium; experimental group - UVB stimulation first and then adding samples; control group - adding serum-free culture medium.

[0115] Senescent cell number clearance rate = (initial senescent cell number - experimental group senescent cell data) / initial senescent cell number * 100%.

[0116] The test results are shown in Table 4 below.

[0117] Table 4

[0118]

[0119]

[0120] It can be seen from the above table that supercritical peony seed oil and oil-soluble peony callus extract fermented by double plant lactobacillus can better remove senescent cells and have more obvious anti-wrinkle effects. From the results in the table, it can be seen that the selection of bacterial strains and high-pressure and high-oxygen conditions are also crucial to the efficacy of the composition.

[0121] Performance Test-4 Anti-wrinkle Test

[0122] Test method: Recruit volunteers aged 35-50 years old and randomly divide them into 14 groups, 8 people in each group. Each group uses the corresponding test samples (cream provided by application examples 1-6, comparative application examples 1-7 and blank application examples) for experiments. Use once in the morning and evening every day, and use the area around the eyes of the volunteers as the test area. After cleansing, wait for 15 minutes in a room with a room temperature of 22℃±2℃ and a relative humidity of 40±2%, and then use the VISIA facial image analyzer to take a photo of the supporter's face. The analysis area should try to avoid interference from reflective areas and hair, and record the initial value of the number of wrinkles A0. After using it continuously for one month, take a photo to record the number of wrinkles A1. Wrinkle reduction rate = wrinkle reduction rate = [(A1-A0) / A0]*100%.

[0123] The average value of the reduction rate of the number of eye wrinkles of each group of volunteers was taken.

[0124] The results are shown in Table 5 below.

[0125] Table 5

[0126]

[0127]

[0128] It can be seen from the above table that the selection of bacterial strains is crucial to the effect of its efficacy. The supercritical peony seed oil fermented by double plant lactobacillus can better synergize with the oil-soluble peony callus extract, and the anti-wrinkle effect is more obvious. At the same time, other influencing factors of high pressure and high oxygen conditions also affect the efficacy of its combination.

[0129] Performance Test-5 Tyrosinase Inhibition Test

[0130] Test method: Take 30 μL of the test sample (cream provided in Application Examples 1-6, Comparative Application Examples 1-7 and Blank Application Examples), prepare it into 1 mL with 970 μL of pH 6.8 phosphate buffer solution, add 1 mL of 0.1 mg / mL tyrosine, then add 1 mL of tyrosinase (mushroom source, 200 u / mg) prepared with pH 6.8 phosphate buffer solution, incubate at 37°C for 20 min, and measure the absorbance at 475 nm.

[0131] The positive control was 0.2 mg / mL kojic acid. Each experiment was repeated three times and the average value was taken as the final result. The tyrosinase activity inhibition rate was determined according to the following formula.

[0132] Tyrosinase activity inhibition rate = 1-[(A3-A4) / (A1-A2)] × 100%;

[0133] A1: absorbance in the absence of tyrosinase inhibitor; A2: absorbance in the presence of tyrosine alone; A3: absorbance of tyrosinase and tyrosine in the presence of inhibitor; A4: absorbance in the absence of tyrosinase.

[0134] The test results are shown in Table 6 below.

[0135] Table 6

[0136]

[0137]

[0138] It can be seen from the above table that the selection of strains is crucial to the effect of the efficacy thereof. The supercritical peony seed oil fermented by Lactobacillus plantarum can better synergize with the oil-soluble peony callus extract, effectively inhibit the activity of tyrosinase, and inhibit the formation of melanin, thereby achieving a whitening effect. It can be seen from the results of Example 1 and Comparative Examples 3-4 that the high pressure and high oxygen conditions also have an effect on the efficacy of the composition.

[0139] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-aging composition, characterized in that The method comprises the following components in parts by weight: 1-10 parts of supercritical fermented peony seed oil and 0.1-1 parts of oil-soluble peony callus extract; The preparation method of supercritical peony fermented seed oil comprises the following steps: adding activated plant lactobacillus bacterial liquid into supercritical peony seed oil under high pressure and high oxygen conditions for fermentation, and centrifugation filtering to obtain supercritical peony fermented seed oil; the high pressure condition is 20-24 MPa, the high oxygen condition is adding fluoride liquid containing saturated high-purity oxygen with a volume concentration of 15-18%; the plant lactobacillus bacterial liquid is a mixture of two different plant lactobacillus bacterial liquids.

2. The anti-aging composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 5-8 parts of supercritical fermented peony seed oil and 0.3-0.5 parts of oil-soluble peony callus extract.

3. The anti-aging composition according to claim 1, characterized in that The method for activating Lactobacillus plantarum is as follows: adding Lactobacillus plantarum into MRS culture medium for activation culture, the culture temperature is 25-37° C., and the culture time is 24-48 hours.

4. The anti-aging composition according to claim 1, characterized in that The volume ratio of the plant lactobacillus liquid to the supercritical peony seed oil is (5-10):

100.

5. The anti-aging composition according to claim 1, characterized in that The fermentation temperature is 23-28°C, and the fermentation time is 24-48h.

6. A method for preparing the anti-aging composition according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: mixing supercritical peony fermented seed oil and oil-soluble peony callus extract to obtain the anti-aging composition.

7. Use of the anti-aging composition according to any one of claims 1 to 5 in the preparation of skin products.

8. A skin care product, characterized in that: The skin product comprises the anti-aging composition according to any one of claims 1 to 5.

9. The skin care product according to claim 8, wherein: The skin products include one of lotion, emulsion, cream, facial mask, essence and spray.

Images