Composition with skin lipid increase and soothing and repairing effects and preparation method thereof
By using specific proportional compositions in cosmetics, including hydrolyzing keratin gum, xanthan gum and other ingredients, the problems existing in existing cosmetics that are difficult for existing cosmetics to enhance the skin lipid barrier and stable in the transparent water agent system are solved, and the stability and efficacy of the product are improved.
Patent Information
- Application Number
- CN202411883871.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2025-04-08
AI Technical Summary
Existing cosmetics are difficult to effectively enhance the skin lipid barrier and exist stably in the transparent water agent system, resulting in poor product stability and efficacy.
A composition is adopted, which includes hydrolyzed Rhizophobia gum, xanthan gum, glycerin, sodium hyaluronate, wheat seed extract, flaxseed extract, lactic acid bacteria fermentation extract, mussel extract and other components. Through scientific proportioning and specific preparation methods, the ingredients are ensured to be uniformly dispersed and stable in existence.
This composition can effectively enhance the skin lipid barrier and provide soothing and repairing effects. It is suitable for transparent water agent systems. It has good product stability, delicate skin feeling and high safety.
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition having the effects of increasing skin lipids and soothing and repairing skin, and a preparation method thereof. Background Art
[0002] The skin is the first physiological barrier of the human body, and lipids are the main structural components of the skin barrier, mainly including squalene, triglycerides, ceramides, free fatty acids and cholesterol. Lipids are mainly divided into two categories. One is the sebaceous gland lipids that are directly secreted by the sebaceous glands to the surface of the skin; the other is the intercellular lipids that are dispersed between the stratum corneum cells as the cells grow and metabolize. Studies have shown that the secretion process of skin lipids and changes in the content, structure or proportion of the main components will affect the skin barrier function. Lipids play an irreplaceable role in maintaining the skin barrier function.
[0003] At present, cosmetics repair damaged skin barriers mainly by exogenously applying moisturizing creams rich in ceramides, free fatty acids, cholesterol, etc. As we all know, ceramides, free fatty acids, cholesterol, etc. are all fat-soluble substances that are insoluble in water. Even ceramide-like substances are only soluble in very few oil solvents, which limits their application in aqueous cosmetics. Water-soluble ceramides and water-soluble cholesterol on the market are generally in the form of liposome encapsulation, nanoemulsions or microemulsions, and fat-soluble substances such as ceramides and cholesterol are encapsulated in the form of opaque mixtures to increase the solubility of fat-soluble substances in aqueous systems. Therefore, they are generally used in non-transparent essences, lotions, and creams, and are difficult to use in transparent aqueous systems. On the other hand, in actual application, water-soluble ceramides and water-soluble cholesterol have stratification or precipitation of fat-soluble substances, which leads to product stability problems and cannot achieve the expected efficacy. In order to solve this problem, it is usually necessary to add a suspending agent. However, if the type and amount of the suspending agent are not properly selected, it will not only fail to play a suspending role, but will also affect the texture of the product, causing the skin to feel thick, sticky, acne-inducing, and even the risk of skin irritation. In addition, some suspending agents will also affect the appearance of the product, causing the product to appear turbid or uneven.
[0004] In addition, the skin is affected by external factors such as seasonal changes, temperature changes, and sun exposure, causing discomfort such as burning, stinging, itching, and tightness. In addition, external cosmetics, facial cleansing products, and increasingly serious environmental pollution further aggravate skin irritation and reduce the skin's ability to resist foreign substances. These irritants trigger a series of inflammatory reactions, and inflammatory factors in turn irritate the skin, further causing barrier damage. Sensitivity and lipid barrier damage are both skin problems that need to be urgently addressed. Summary of the invention
[0005] The technical problem to be solved by the present invention is to provide a composition with the effects of increasing skin lipids and soothing and repairing and a preparation method thereof in view of the deficiencies of the above-mentioned prior art. The composition of the present invention can effectively enhance the skin lipid barrier, and at the same time can soothe and repair sensitive skin, and reduce the further damage to the skin barrier due to skin sensitivity. It can be applied to emulsions, creams / ointments, and especially to transparent aqueous preparations. The product is safe, stable, and has a delicate skin feel.
[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: a composition with the effects of increasing skin lipids and soothing and repairing, characterized in that, based on 100 parts by weight, the composition comprises:
[0007] 0.2-0.4 parts by weight of hydrolyzed sclerotium gum, 0.05-0.2 parts by weight of xanthan gum, 3-8 parts by weight of glycerin, 0.05-0.2 parts by weight of sodium hyaluronate, 0.01-0.05 parts by weight of wheat seed extract, 0.005-0.01 parts by weight of flaxseed extract, 0.1-1 parts by weight of lactic acid bacteria fermentation extract, 0-0.01 parts by weight of mussel extract, 0.2-4 parts by weight of butylene glycol, 0.4-1 parts by weight of 1,2-hexanediol, 0.1-0.4 parts by weight of p-hydroxyacetophenone, 0.02-0.08 parts by weight of caprylhydroxamic acid, 0.005-0.02 parts by weight of ethylhexylglycerin, and the remainder is water.
[0008] The above-mentioned composition with the effects of increasing skin lipids and soothing and repairing is characterized in that the mass ratio of the wheat seed extract to the flax seed extract is (1-10):1.
[0009] The above-mentioned composition with the effects of increasing skin lipids and soothing and repairing is characterized in that the mass ratio of the hydrolyzed sclerotium gum to xanthan gum is (1-8):1.
[0010] The above-mentioned composition with the effects of increasing skin lipids and soothing and repairing is characterized in that the preparation method of the wheat seed extract includes: washing and crushing the wheat seeds to 45-60 mesh, then adding them into 20-45 times water and soaking them for 2h-3h, ultrasonicating them in a constant temperature water bath for 20min-35min, stirring and extracting them for 1h-2h, collecting the supernatant, adding a 50%-60% ethanol solution to the collected supernatant, stirring and extracting them at room temperature for 2-3 times, each extraction time is 1h-2h, centrifuging to separate the precipitate, and vacuum freeze-drying the separated precipitate to obtain the wheat seed extract.
[0011] The above-mentioned composition with the effects of increasing skin lipids and soothing and repairing is characterized in that the temperature of the constant temperature water bath ultrasound is 45°C to 55°C.
[0012] The above-mentioned composition with the effects of increasing skin lipids and soothing and repairing skin is characterized in that the DOPA content of the mussel extract is not less than 0.3%.
[0013] Furthermore, the present invention provides a method for preparing the above-mentioned composition having the effects of increasing skin lipids and soothing and repairing skin, characterized in that it comprises:
[0014] Step 1: Preprocessing, including:
[0015] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0016] Step 102, adding wheat seed extract to 50° C. to 55° C. water, stirring evenly, and when the temperature drops below 45° C., adding flax seed extract, stirring evenly, to obtain a reserve solution 2;
[0017] Step 103, adding the mussel extract and the lactic acid bacteria fermentation extract into the water, stirring evenly, to obtain a reserve solution 3;
[0018] Step 2, add the reserve solution 1 to the water at 70℃~75℃, set the stirring speed to 700r / min~900r / min, stir evenly and keep warm to let it swell until it is completely transparent, and stir and mix evenly;
[0019] Step 3: When the temperature of the material in step 2 drops to 40°C to 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir until mixed, filter, and obtain a composition with the effects of increasing skin lipids and soothing and repairing.
[0020] The above method is characterized in that the heat preservation and standing time in step 2 is 20 minutes to 30 minutes.
[0021] Compared with the prior art, the present invention has the following advantages:
[0022] 1. The composition of the present invention has the effects of increasing skin lipids and soothing and repairing skin. The raw materials are scientifically proportioned and work synergistically. The skin lipid barrier strengthening component composed of wheat seed extract and flax seed extract is combined with mussel extract and lactic acid bacteria fermentation extract to construct a composition that can effectively enhance the skin lipid barrier and achieve soothing and repairing effects on sensitive skin. The composition is transparent and has excellent water solubility, and can be applied to a transparent aqueous system; and the stability of the product can be ensured without the use of a suspending agent, and there are no safety hazards caused by a suspending agent, etc. The product is safe to use, has stable performance, and has a delicate skin feel.
[0023] 2. The composition with the effects of increasing skin lipids and soothing and repairing of the present invention fully combines the structural characteristics of wheat seed extract to protect the lipids of the stratum corneum of the skin epidermis and flaxseed extract to supply long-chain fatty acids and phytosterols, and jointly supplements skin lipids, so that the skin lipid structure can be fully supplemented, and the skin barrier is further stabilized and strengthened; on the other hand, the soothing component mainly composed of mussel extract and lactic acid bacteria fermentation extract can effectively achieve skin repair, anti-oxidation, and enhance the skin's ability to resist photoaging, so that the composition with the effects of increasing skin lipids and soothing and repairing of the present invention can effectively enhance the skin lipid barrier, and at the same time can soothe and repair sensitive skin, and reduce the further damage to the skin barrier due to skin sensitivity.
[0024] 3. The preparation method of the wheat seed extract of the present invention controls the preparation parameters and processes so that the obtained wheat seed extract has excellent skin lipid barrier repairing effect, and is used in combination with flaxseed extract in the composition of the present invention to fully exert the effect of enhancing the skin lipid barrier; the preparation method of the composition with skin lipid increase and soothing and repairing effects provided by the present invention adopts the method of fully keeping some raw materials warm and letting them stand before adding the remaining ingredients, which can effectively achieve uniform dispersion of various active ingredients in the product and ensure the efficacy and stability of the product.
[0025] 4. The method of the present invention for preparing a composition having the effects of increasing skin lipids and soothing and repairing skin has the characteristics of low thickener dosage, short preparation process time, low energy consumption and low cost, which is conducive to promotion and application.
[0026] The technical solution of the present invention is further described in detail below in conjunction with embodiments. DETAILED DESCRIPTION
[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the embodiments. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following embodiments, unless otherwise specified, can be obtained from commercial channels.
[0028] The invention provides a composition with skin lipid increase and soothing and repairing effects. The composition comprises, based on 100 parts by weight, 0.2-0.4 parts by weight of hydrolyzed sclerotium gum, 0.05-0.2 parts by weight of xanthan gum, 3-8 parts by weight of glycerol, 0.05-0.2 parts by weight of sodium hyaluronate, 0.01-0.05 parts by weight of wheat seed extract, 0.005-0.01 parts by weight of flaxseed extract, 0.1-1 parts by weight of lactic acid bacteria fermentation extract, 0-0.01 parts by weight of mussel extract, 0.2-4 parts by weight of butylene glycol, 0.4-1 parts by weight of 1,2-hexanediol, 0.1-0.4 parts by weight of p-hydroxyacetophenone, 0.02-0.08 parts by weight of caprylhydroxamic acid, 0.005-0.02 parts by weight of ethylhexylglycerol, and the remainder being water.
[0029] In the composition, wheat seed extract and flax seed extract are used as water-soluble plant biomimetic lipids. Wheat seed extract is rich in glucosylceramide, which is similar in structure to ceramide 3 and ceramide 4 in the human body and can play a protective role in the lipids of the stratum corneum of the skin epidermis. Flax seed extract is rich in α-linolenic acid, phytosterols, tocopherol, etc., and can provide long-chain fatty acids and phytosterols. Multiple active ingredients jointly supplement skin lipids, so that the skin lipid structure is fully supplemented, and the barrier effect is further stabilized and enhanced.
[0030] The main function of mussel extract and lactic acid bacteria fermentation extract is soothing. The mussel mucin sequence in the mussel extract contains hydrophobic amino acids, which can achieve water-proof attachment. Mussel mucin is positively charged under weakly acidic conditions and combines with negatively charged skin or cells through electrostatic action to accelerate skin repair. The dopa group in mussel mucin has a strong antioxidant effect and can effectively deal with oxidative damage such as ultraviolet rays. Lactic acid bacteria fermentation extract, the main component of which is extracellular polysaccharides, has strong biological activities such as antioxidant activity, immunomodulatory activity, anti-inflammatory activity and anti-biofilm activity, which can enhance the skin's ability to resist photoaging, slow down skin photodamage induced by ultraviolet rays, and promote the repair of the skin's microecological barrier.
[0031] The main function of hydrolyzed sclerotium gum and xanthan gum is thickening.
[0032] Glycerin, sodium hyaluronate, butylene glycol and 1,2-hexanediol mainly play a role in moisturizing.
[0033] Parahydroxyacetophenone, caprylhydroxamic acid, and ethylhexylglycerin are primarily skin conditioning agents.
[0034] The above ingredients cooperate with each other to form the composition of the present invention which has the effects of increasing skin lipids and soothing and repairing skin.
[0035] The present invention is specifically described below in conjunction with embodiments, but the following description is not intended to limit the present invention.
[0036] Example 1
[0037] This embodiment provides a composition having the effects of increasing skin lipids and soothing and repairing skin. The mass of the composition is 100 g, and the formula of the composition includes:
[0038] 0.2g hydrolyzed sclerotium gum, 0.2g xanthan gum, 3g glycerin, 0.05g sodium hyaluronate, 0.015g wheat seed extract, 0.005g flax seed extract, 0.6g lactic acid bacteria fermentation extract, 4g butylene glycol, 0.4g 1,2-hexanediol, 0.1g p-hydroxyacetophenone, 0.08g caprylhydroxamic acid, 0.005g ethylhexylglycerin, and the rest is water.
[0039] The present embodiment provides a method for preparing a wheat seed extract, comprising: washing and crushing wheat seeds into 45-60 meshes, then adding the seeds into 20 times water and soaking them for 2 hours, performing ultrasonic treatment in a constant temperature water bath of 45° C. for 20 minutes, and then performing extraction by stirring for 1 hour, collecting the supernatant, adding a 50% ethanol solution to the collected supernatant, performing extraction by stirring for 2 times at room temperature, each extraction time being 1 hour, separating the precipitate by centrifugation, and placing the separated precipitate in a vacuum freeze dryer for vacuum freeze drying to obtain the wheat seed extract.
[0040] This embodiment provides a method for preparing a composition having the effects of increasing skin lipids and soothing and repairing skin, comprising:
[0041] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0042] Step 102, weigh 4% of the water by mass of the formula water, heat it to 50° C., add the wheat seed extract under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract, stir and mix evenly, and obtain a transparent standby solution 2;
[0043] Step 103, weigh 4% of the mass of the formula water, add the lactic acid bacteria fermentation extract preparation, and stir until mixed evenly to obtain a transparent reserve solution 3;
[0044] Step 2: Take the remaining water, heat it to 70°C, add the reserve solution 1, set the stirring speed to 700r / min, stir for 10min, then keep it warm and let it stand for 20min to make it swell until it is completely transparent, stir until the material is evenly mixed, and cool it down;
[0045] Step 3: When the temperature drops to 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0046] Example 2
[0047] This example is the same as Example 1, except that the amount of hydrolyzed sclerotium gum added is 0.3 g, and the amount of xanthan gum added is 0.075 g.
[0048] Example 3
[0049] This example is the same as Example 1, except that the amount of hydrolyzed sclerotium gum added is 0.4 g, and the amount of xanthan gum added is 0.05 g.
[0050] Example 4
[0051] This embodiment provides a composition having the effects of increasing skin lipids and soothing and repairing skin. The mass of the composition is 100 g, and the formula of the composition includes:
[0052] 0.4g hydrolyzed sclerotium gum, 0.2g xanthan gum, 8g glycerin, 0.2g sodium hyaluronate, 0.05g wheat seed extract, 0.005g flax seed extract, 0.8g lactic acid bacteria fermentation extract, 2g butylene glycol, 0.8g 1,2-hexanediol, 0.25g p-hydroxyacetophenone, 0.05g caprylhydroxamic acid, 0.02g ethylhexylglycerin, and the rest is water.
[0053] The present embodiment provides a method for preparing a wheat seed extract, comprising: washing and crushing wheat seeds into 45-60 meshes, then adding the seeds into 32 times water and soaking them for 2.5 hours, ultrasonicating them in a water bath at a constant temperature of 50° C. for 28 minutes, stirring and extracting them for 1.5 hours, collecting the supernatant, adding a 55% ethanol solution to the collected supernatant, stirring and extracting them twice at room temperature, each extraction time being 1.5 hours, separating the precipitate by centrifugation, and placing the separated precipitate in a vacuum freeze dryer for vacuum freeze drying to obtain the wheat seed extract.
[0054] This embodiment provides a method for preparing a composition having the effects of increasing skin lipids and soothing and repairing skin, comprising:
[0055] Step 1: Preprocessing, including:
[0056] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0057] Step 102, weigh 4% of the water of the formula water, heat it to 52° C., add the wheat seed extract preparation under stirring, continue to stir evenly, and when the temperature drops to below 45° C., add the flax seed extract preparation, stir and mix evenly to obtain a transparent standby solution 2;
[0058] Step 103, weigh 3% of the mass of the formula water, add the lactic acid bacteria fermentation extract preparation, stir and mix evenly to obtain a transparent reserve solution 3;
[0059] Step 2: Take the remaining water, heat it to 75°C, add the reserve solution 1, set the stirring speed to 800r / min, stir for 10min, then keep it warm and let it stand for 25min to make it swell until it is completely transparent, stir until the material is evenly mixed, and cool it down;
[0060] Step 3: When the temperature drops to 42°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition with the effects of increasing skin lipids and soothing and repairing.
[0061] Example 5
[0062] This example is the same as Example 4, except that the added amount of wheat seed extract is 0.04 g, and the added amount of flax seed extract is 0.008 g.
[0063] Example 6
[0064] This example is the same as Example 4, except that the added amount of wheat seed extract is 0.01 g, and the added amount of flax seed extract is 0.01 g.
[0065] Example 7
[0066] This embodiment provides a composition having the effects of increasing skin lipids and soothing and repairing skin. The mass of the composition is 100 g, and the formula of the composition includes:
[0067] 0.3g hydrolyzed sclerotium gum, 0.16g xanthan gum, 6g glycerin, 0.11g sodium hyaluronate, 0.05g wheat seed extract, 0.005g flax seed extract, 0.1g lactic acid bacteria fermentation extract, 0.2g butylene glycol, 1g 1,2-hexanediol, 0.4g p-hydroxyacetophenone, 0.02g caprylhydroxamic acid, 0.008g ethylhexylglycerin, and the rest is water.
[0068] The present embodiment provides a method for preparing a wheat seed extract, comprising: washing and crushing wheat seeds into 45-60 mesh sizes, then adding the seeds into 45 times water and soaking them for 3 hours, performing ultrasonic treatment in a constant temperature water bath of 55° C. for 35 minutes, and then performing extraction by stirring for 2 hours, collecting the supernatant, adding a 60% ethanol solution to the collected supernatant, and performing extraction by stirring for 3 times at room temperature, each extraction time being 2 hours, separating the precipitate by centrifugation, and placing the separated precipitate in a vacuum freeze dryer for vacuum freeze drying to obtain the wheat seed extract.
[0069] This embodiment provides a method for preparing a composition having the effects of increasing skin lipids and soothing and repairing skin, comprising:
[0070] Step 1: Preprocessing, including:
[0071] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0072] Step 102, weigh 5% of the water by mass of the formula water, heat it to 55° C., add the wheat seed extract preparation under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract preparation, stir and mix evenly, and obtain a transparent standby solution 2;
[0073] Step 103, weigh 5% of the water by mass of the formula water, add the lactic acid bacteria fermentation extract preparation, and stir until mixed evenly to obtain a transparent reserve solution 3;
[0074] Step 2: Take the remaining water, heat it to 72°C, add the reserve solution 1, set the stirring speed to 900r / min, stir for 10min, then keep it warm and let it stand for 30min to make it swell until it is completely transparent, stir until the material is evenly mixed, and cool it down;
[0075] Step 3: When the temperature drops to 40°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0076] Example 8
[0077] This embodiment is the same as embodiment 7, except that the added amount of the lactic acid bacteria fermentation extract is 0.5 g.
[0078] Example 9
[0079] This embodiment is the same as Embodiment 7, except that the amount of lactic acid bacteria fermentation extract added is 1 g.
[0080] Example 10
[0081] This embodiment is the same as embodiment 5, except that: the formula includes 0.01 g of mussel extract, 5% of the mass of the water in the formula is weighed in step 103, and the mussel extract and the lactic acid bacteria fermentation extract preparation are added respectively, and stirred until mixed evenly to obtain a transparent reserve solution 3.
[0082] Furthermore, the DOPA content of the mussel extract is 0.5%.
[0083] The preparation method of the wheat seed extract and the preparation method of the composition in this example are consistent with those in Example 5.
[0084] Embodiment 11
[0085] This embodiment is the same as embodiment 5, except that: the formula includes 0.005 g of mussel extract, 5% of the mass of the water in the formula is weighed in step 103, and the mussel extract and the lactic acid bacteria fermentation extract preparation are added respectively, and stirred until mixed evenly to obtain a transparent reserve solution 3.
[0086] Furthermore, the DOPA content of the mussel extract is 0.5%.
[0087] The rest is consistent with Example 5.
[0088] Comparative Example 1
[0089] The composition formula and preparation process of the composition of this comparative example are consistent with those of Example 5, except that the preparation method of the wheat seed extract of this comparative example is different from that of Example 5.
[0090] This comparative example provides a method for preparing a wheat seed extract, comprising:
[0091] Weigh wheat seeds, wash and crush to 45-60 mesh size, take the pre-treated wheat seed powder and add it to 32 times water, soak for 2.5 hours. Constant temperature 40℃ water bath ultrasonic for 28 minutes, then stir and extract for 1.5 hours, collect the supernatant, add 55% ethanol solution, stir and extract twice at room temperature, each extraction time is 1.5 hours, centrifuge to separate the precipitate, place it in a vacuum freeze dryer for drying to obtain a solid powder, which is the desired wheat seed extract.
[0092] Comparative Example 2
[0093] The composition formula and preparation process of the composition of this comparative example are consistent with those of Example 5, except that the preparation method of the wheat seed extract of this comparative example is different from that of Example 5.
[0094] This comparative example provides a method for preparing a wheat seed extract, comprising:
[0095] Weigh wheat seeds, wash them, and crush them into 45-60 mesh size. Take the pretreated wheat seed powder and add it into 32 times of water, soak for 2.5 hours, ultrasonicate in a constant temperature 60℃ water bath for 28 minutes, and then stir and extract for 1.5 hours. Collect the supernatant, add 55% ethanol solution, stir and extract twice at room temperature, each extraction time is 1.5 hours, separate the precipitate by centrifugation, and dry it in a vacuum freeze dryer to obtain a solid powder, which is the required wheat seed extract.
[0096] Comparative Example 3
[0097] The composition formula and preparation process of the composition of this comparative example are consistent with those of Example 5, except that the preparation method of the wheat seed extract of this comparative example is different from that of Example 5.
[0098] This comparative example provides a method for preparing a wheat seed extract, comprising:
[0099] Weigh wheat seeds, wash them, and crush them into 45-60 mesh size. Take the pretreated wheat seed powder and add it into 32 times of water, soak for 2.5 hours, ultrasonicate in a constant temperature 60℃ water bath for 15 minutes, and then stir and extract for 1.5 hours. Collect the supernatant, add 55% ethanol solution, stir and extract twice at room temperature, each extraction time is 1.5 hours, separate the precipitate by centrifugation, and dry it in a vacuum freeze dryer to obtain a solid powder, which is the required wheat seed extract.
[0100] Comparative Example 4
[0101] The composition formula and preparation process of the composition of this comparative example are consistent with those of Example 5, except that the preparation method of the wheat seed extract of this comparative example is different from that of Example 5.
[0102] This comparative example provides a method for preparing a wheat seed extract, comprising:
[0103] Weigh wheat seeds, wash them, and crush them into 45-60 mesh size. Take the pretreated wheat seed powder and add it into 32 times of water, soak for 2.5 hours, ultrasonicate in a constant temperature 60℃ water bath for 40 minutes, and then stir and extract for 1.5 hours. Collect the supernatant, add 55% ethanol solution, stir and extract twice at room temperature, each extraction time is 1.5 hours, separate the precipitate by centrifugation, and dry it in a vacuum freeze dryer to obtain a solid powder, which is the required wheat seed extract.
[0104] Comparative Example 5
[0105] The composition formula and the preparation method of the wheat seed extract of this comparative example are consistent with those of Example 5.
[0106] This comparative example provides a method for preparing the above-mentioned composition, comprising:
[0107] Step 1: Preprocessing, including:
[0108] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0109] Step 102, weigh 4% of the water by mass of the formula water, heat it to 45° C., add the wheat seed extract preparation under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract preparation, stir and mix evenly, and obtain a transparent standby solution 2;
[0110] Step 103, weigh 3% of the water by mass of the formula water, add the mussel extract and the lactic acid bacteria fermentation extract preparation respectively, and stir until the mixture is uniform to obtain a transparent reserve solution 3;
[0111] Step 2: Take the remaining water, heat it to 75°C, add the reserve solution 1, set the stirring speed to 800r / min, stir for 10min, then keep it warm and let it stand for 25min to make it swell until it is completely transparent, stir until the material is evenly mixed, and cool it down;
[0112] Step 3: When the temperature drops below 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0113] Comparative Example 6
[0114] The composition formula and preparation process of the wheat seed extract in this comparative example are consistent with those in Example 5.
[0115] This comparative example provides a method for preparing the above-mentioned composition, comprising:
[0116] Step 1: Preprocessing, including:
[0117] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0118] Step 102, weigh 4% of the water by mass of the formula water, heat it to 60° C., add the wheat seed extract preparation under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract preparation, stir and mix evenly, and obtain a transparent standby solution 2;
[0119] Step 103, weigh 3% of the water by mass of the formula water, add the mussel extract and the lactic acid bacteria fermentation extract preparation respectively, and stir until the mixture is uniform to obtain a transparent reserve solution 3;
[0120] Step 2: Take the remaining water, heat it to 75°C, stop heating, add the reserve solution 1, set the stirring speed to 800r / min, keep it warm and let it stand for 25min until the material is evenly mixed, and cool it down;
[0121] Step 3: When the temperature drops below 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0122] Comparative Example 7
[0123] The composition formula and preparation process of the wheat seed extract in this comparative example are consistent with those in Example 5.
[0124] This comparative example provides a method for preparing the above-mentioned composition, comprising:
[0125] Step 1: Preprocessing, including:
[0126] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0127] Step 102, weigh 4% of the water by mass of the formula water, heat it to 52° C., add the wheat seed extract preparation under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract preparation, stir and mix evenly, and obtain a transparent standby solution 2;
[0128] Step 103, weigh 3% of the water by mass of the formula water, add the mussel extract and the lactic acid bacteria fermentation extract preparation respectively, and stir until the mixture is uniform to obtain a transparent reserve solution 3;
[0129] Step 2: Take the remaining water, heat it to 75°C, stop heating, add the reserve solution 1, set the stirring speed to 800r / min until the material is evenly mixed, and cool it down;
[0130] Step 3: When the temperature drops below 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0131] Comparative Example 8
[0132] The composition formula and preparation process of the wheat seed extract in this comparative example are consistent with those in Example 5.
[0133] This comparative example provides a method for preparing the above-mentioned composition, comprising:
[0134] Step 1: Preprocessing, including:
[0135] Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1;
[0136] Step 102, weigh 4% of the water by mass of the formula water, heat it to 52° C., add the wheat seed extract preparation under stirring, continue to stir evenly, wait until the temperature drops below 45° C., add the flax seed extract preparation, stir and mix evenly, and obtain a transparent standby solution 2;
[0137] Step 103, weigh 3% of the water by mass of the formula water, add the mussel extract and the lactic acid bacteria fermentation extract preparation respectively, and stir until the mixture is uniform to obtain a transparent reserve solution 3;
[0138] Step 2: Take the remaining water, heat it to 75°C, add the reserve solution 1, set the stirring speed to 800r / min, stir for 10min, keep warm and let it stand for 15min until the material is evenly mixed, and cool it down;
[0139] Step 3: When the temperature drops below 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir for 15 minutes until the mixture is evenly mixed, and filter to obtain a composition having the effects of increasing skin lipids and soothing and repairing.
[0140] Comparative Example 9
[0141] Compared with Example 5, the difference between this comparative example and Example 5 is that the amount of hydrolyzed sclerotium gum added in the formula is 0.1g, and the amount of xanthan gum added in the formula is 0.01g;
[0142] The preparation method of the wheat seed extract and the preparation method of the composition in this comparative example are consistent with those in Example 5.
[0143] Comparative Example 10
[0144] This comparative example is basically the same as Example 5, except that the wheat seed extract and flax seed extract in Example 5 are replaced by ceramide 3, and the added amount of ceramide 3 is 0.048 g; the model of the ceramide 3 is Ceramide IIIB, which is purchased from evonik operations gmbh.
[0145] The preparation method of the composition is consistent with that of Example 5.
[0146] Comparative Example 11
[0147] This comparative example is consistent with Example 5, except that the mass of the wheat seed extract in the formula is 0.007 g, the mass of the flax seed extract is 0.008 g, and the remaining ingredients are the same as those in Example 5.
[0148] The preparation method of the wheat seed extract and the preparation method of the composition in this comparative example are consistent with those in Example 5.
[0149] Comparative Example 12
[0150] This comparative example is consistent with Example 5, except that the mass of the wheat seed extract in the formula is 0.05 g, the mass of the flax seed extract is 0.003 g, and the other ingredients are the same as those in Example 5.
[0151] The preparation method of the wheat seed extract and the preparation method of the composition in this comparative example are consistent with those in Example 5.
[0152] Comparative Example 13
[0153] This comparative example is basically the same as Example 9, except that no lactic acid bacteria fermentation extract is added.
[0154] The preparation method of the wheat seed extract and the preparation method of the composition of this comparative example are consistent with those of Example 9.
[0155] Experimental example
[0156] 1. Efficacy evaluation
[0157] The performance of the above composition was evaluated with reference to the "China Association for Promoting Industry-University-Research Cooperation Group Standard T / CAB 0152-2022 Test Methods for Seven Efficacy of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing", as follows:
[0158] 1.1 Selection of subjects:
[0159] (1) Select volunteers aged 18-60 who meet the experimental requirements as subjects;
[0160] (2) Subjects with the following conditions cannot be selected as subjects: those who have used antihistamines in the past week or immunosuppressants in the past month; those who have used any anti-inflammatory drugs on the test site in the past two months; subjects with inflammatory skin diseases that have not been cured clinically; patients with insulin-dependent diabetes; patients with asthma or other chronic respiratory diseases being treated; those who have received anti-cancer chemotherapy in the past 6 months; patients with immunodeficiency or autoimmune diseases; breastfeeding or pregnant women; those who have undergone bilateral mastectomy and bilateral axillary lymph node resection; those whose skin test sites have scars, pigmentation, atrophy, port-wine stains or other blemishes that affect the determination of the test results; those participating in other clinical trial researchers; those with highly sensitive constitutions; non-voluntary participants or those who cannot complete the specified content as required by the trial.
[0161] 1.2 Skin lipid content test
[0162] Instrument: German CK-Skin Oil Test Probe Sebumeter SM815
[0163] Test area: forehead and left and right sides of nose
[0164] Methods: Recruit participants to participate in the efficacy test. According to statistical principles, the sample area and control area were randomly distributed on the left and right sides of the face. Within 3 minutes after the subject cleaned the face, a 3*3cm test area was marked above the left and right eyebrows of the subject, and the skin oil content test was performed as the basic value T0. 40μL of the test sample was used once on half of the face and evenly applied to the test area with a latex fingertip. Data on the skin oil content at different measurement locations were collected 1h and 2h after using the product. When measuring at different measurement time nodes, the test area above the eyebrows was measured 3 times and the nose wing was measured once. The average value was taken as the measurement result and statistical analysis was performed.
[0165] Table 1 Test results of skin lipid content in examples and comparative examples
[0166] Example D0 D7 D14 D28 Example 1 56.47 79.47 100.37 128.89 Example 2 57.34 78.56 100.28 132.74 Example 3 58.01 80.22 100.71 131.62 Example 4 57.29 79.52 100.26 129.31 Example 5 56.47 80.47 102.33 133.90 Example 6 56.52 78.40 100.81 130.38 Example 7 57.10 79.71 100.23 130.40 Example 8 56.88 79.03 99.96 130.29 Example 9 56.61 78.96 100.47 130.43 Comparative Example 1 57.83 63.53 65.25 68.74 Comparative Example 2 56.91 65.38 65.73 65.06 Comparative Example 3 58.02 64.63 64.42 67.92 Comparative Example 4 57.28 65.37 66.38 67.76 Comparative Example 5 56.47 64.65 66.48 67.90 Comparative Example 6 57.01 65.32 67.03 67.95 Comparative Example 7 56.65 65.10 66.82 68.29 Comparative Example 8 57.61 65.90 67.57 67.20 Comparative Example 11 57.00 65.33 66.70 68.56 Comparative Example 12 56.87 66.11 67.29 68.27
[0167] It can be seen from Table 1 that the increase in skin lipid content in the examples is significantly higher than that in the comparative examples. It can be seen that the composition of the present invention can significantly increase the skin lipid content as the use time increases.
[0168] Comparison of Comparative Examples 1-4 with Example 5 shows that the ultrasonic temperature in the preparation of wheat seed extract is lower or higher than the temperature range recorded in the present invention, and the ultrasonic time is short or too long, which will significantly reduce the efficacy of the formula composition of the present invention. This shows that the preparation method of the wheat seed extract directly affects the efficacy of the raw materials, thereby reducing the efficacy of the formula composition. Comparison of Comparative Examples 5-8 with Example 5 shows that the skin lipid content in Comparative Examples 5-8 is not significantly increased, which shows that the preparation method of the composition of the present invention will also affect its final efficacy. The main reason is that the wheat seed extract extracted by the present invention has good solubility only at an appropriate temperature, and because its solubilized product is transparent, it is difficult to observe obvious undissolved phenomena. If it is not completely dissolved before being added to the system, it cannot exert all the effects and will cause poor uniformity of the composition; but when the temperature is too high, the active ingredient is very easy to lose its activity. The present invention controls the preparation method of wheat seed extract and the preparation method of the composition, so that the obtained wheat seed extract has excellent skin lipid barrier repairing effect, and the wheat seed extract is used in the composition of the present invention in combination with flaxseed extract to fully exert the effect of enhancing the skin lipid barrier; in the preparation method of the composition, the thickener mainly composed of hydrolyzed sclerotium gum and xanthan gum is fully swollen by keeping warm and standing, and then the remaining ingredients are added, which can effectively achieve uniform dispersion of various active ingredients in the product, thereby ensuring the efficacy and stability of the product.
[0169] Compared with Example 5, the addition ratio of wheat seed extract and flax seed extract in Comparative Examples 11-12 is different. The composition prepared in Comparative Examples 11-12 has a very limited effect on promoting the skin lipid content.
[0170] 1.3. Soothing and repairing test
[0171] Instruments: Skin melanin and hemoglobin test probe Mexameter MX18, skin moisture test probe Corneometer CM825, transepidermal water loss and heat loss test probe Tewameter TM Hex
[0172] Test method: After the volunteers cleaned their faces for 20 minutes, the facial skin hemoglobin content, TEWL (transepidermal water loss) and skin stratum corneum moisture content (MMA) were tested as the initial value. After the volunteers used the test samples for 28 consecutive days, the hemoglobin content, TEWL and MMA data were collected again. The data were analyzed using IBM SPSS Statistics software and t-test. The statistical method significance level was P<0.05. Note: During this period, the subjects were not allowed to use other products with soothing and repairing effects, and were not allowed to apply other irritating products on the face.
[0173] Table 2 MMA values of embodiments and comparative examples
[0174] Example D0 D7 D14 D28 Example 1 38.41 52.37 60.85 73.37 Example 2 37.84 52.74 61.75 72.50 Example 3 38.52 52.90 62.09 73.69 Example 4 36.98 51.72 61.93 72.84 Example 5 37.51 53.36 65.83 76.78 Example 6 36.75 51.78 61.77 72.75 Example 7 37.40 52.64 62.23 72.89 Example 8 37.62 52.69 61.97 73.25 Example 9 36.91 51.99 61.59 72.73 Example 10 36.86 51.34 62.00 73.45 Embodiment 11 37.02 52.35 62.16 74.59 Comparative Example 1 38.77 40.90 42.21 45.95 Comparative Example 2 38.90 40.22 42.56 45.83 Comparative Example 3 37.65 39.98 44.48 46.69 Comparative Example 4 38.10 40.63 44.51 46.70 Comparative Example 5 37.52 40.68 44.50 46.21 Comparative Example 6 38.48 39.99 44.47 45.84 Comparative Example 7 38.80 40.52 44.50 45.38 Comparative Example 8 38.56 40.27 44.03 45.89 Comparative Example 11 38.47 40.29 44.78 46.17 Comparative Example 12 38.77 39.96 44.83 45.28
[0175] In order to further prove the barrier enhancement effect of the composition, we tested the effect of the composition on the moisture content of the skin. As can be seen from Table 2, the MMA values of Examples 1-11 increased significantly, increasing by more than 90% on the 28th day, while the skin moisture content in Comparative Examples 1-8 increased very little, which shows that when the preparation process of the wheat seed extract or the preparation process of the composition is changed, the composition has no obvious effect on the increase of the moisture content of the skin. This is mainly because changing the preparation process of the wheat seed extract and the composition not only affects the activity of the wheat seed extract, but also makes the active ingredients not better utilized by the skin; and combined with the data in Table 1, it can be seen that the preparation process affects the skin lipid content, does not play a role in enhancing the barrier, and also causes the skin to have a low moisture content. Compared with Example 5, when the ratio of wheat seed extract to flaxseed extract in the water-soluble plant bionic lipid is not within the range, it is impossible to promote or supplement skin lipids to prevent skin moisture loss, so the skin moisture content is low.
[0176] Table 3 TEWL results of examples and comparative examples
[0177]
[0178]
[0179] Trans Epidermal Water Loss (TEWL), also known as transdermal water loss, is a common indicator reflecting the barrier function of the stratum corneum and is often used to characterize the repair effect of cosmetics. As can be seen from Table 3, the TEWL values of Examples 1-9 were negative on the 14th day, indicating that the skin barrier was enhanced, indicating that the composition provided by the present invention has a significant repair effect. However, the TEWL of the comparative example did not reach a negative value at 28 days, and its repair effect was not obvious. Among them, the repair effect of comparative example 10 was the worst, mainly because ceramide 3 could not be dissolved in the system, resulting in it being difficult to be absorbed when applied on the skin surface, and unable to exert its repair effect.
[0180] Table 4 Heme content variation values of the embodiments and comparative examples
[0181]
[0182]
[0183] According to Table 4, in terms of hemoglobin content, as time goes by, the hemoglobin content of Examples 1-11 shows a decreasing trend, decreasing by more than 9%, while the hemoglobin content of Comparative Examples 1-4 has hardly changed, indicating that the wheat seed extract extracted by the preparation process of the wheat seed extract described in the comparative example is affected in its effective ingredients and component activity, resulting in poor repair effect of the composition; and it can be seen from Comparative Examples 5-8 that the preparation method of the composition of the present invention will also affect its repair effect. The wheat seed extract and flaxseed extract in Comparative Examples 11-12 are not within the scope of the present invention, and the effect of the composition on enhancing the skin barrier function is limited. Skin with poor skin barrier has strong permeability and is more susceptible to stimulation by foreign substances, causing inflammation, increased vascular permeability, and further increased sensitivity, so its hemoglobin content has not been significantly reduced. Compared with Examples 1-9, it can be seen that Examples 10-11 add a certain amount of mussel extract with a DOPA content greater than 0.3%, and the composition has excellent soothing effect. This is mainly because the mussel extract can rely on the DOPA group therein to form a breathable film on the skin surface, thereby exerting a better anti-inflammatory and antipruritic effect. Therefore, the soothing and repairing effects of Examples 10-11 with high DOPA content are more significant. Comparing Examples 7-9 and Comparative Example 13, it can be clearly seen that the addition of lactic acid bacteria fermentation extract can enhance the soothing effect of the composition. The main reason is its anti-inflammatory and soothing effect, which can quickly respond to inflammatory factors appearing in the skin, avoid inflammation from causing further damage to the skin barrier, and enhance the repairing effect of wheat seed extract and flaxseed extract in water-soluble plant bionic lipids on the skin barrier.
[0184] The above efficacy data show that the composition of the present invention has the effects of increasing skin lipids and soothing and repairing skin. The raw materials are scientifically proportioned and work synergistically. The wheat seed extract protects the lipids in the stratum corneum of the skin epidermis and the flaxseed extract supplies the structural characteristics of long-chain fatty acids and phytosterols to form a skin lipid barrier strengthening component, which jointly replenishes skin lipids and firmly strengthens the skin barrier; and combined with mussel extract and lactic acid bacteria fermentation extract, a composition is constructed that can effectively enhance the skin lipid barrier and achieve the soothing and repairing effect on sensitive skin. The composition is transparent and has excellent water solubility, and can be suitable for a transparent aqueous system.
[0185] 1.4 Lactic acid sting test
[0186] Screening for lactic acid stinging: At room temperature, the test was conducted on the nasolabial folds of the subjects. 50 μL of 10% lactic acid solution was dropped on a single-layer filter paper with a diameter of 0.8 cm. The subjects were asked about their perceived stinging sensation at 30, 2.5, and 5 minutes, respectively. The scores were scored and recorded using a 4-point method (0 for no stinging, 1 for mild stinging, 2 for moderate stinging, and 3 for severe stinging). When the accumulated stinging sensation was ≥ 3 points, it was lactic acid stinging. The subjects used the samples for 28 days as required, and the lactic acid stinging test was repeated 28 days after using the samples.
[0187] Table 5 Lactic acid stinging test results of the embodiments and comparative examples
[0188]
[0189]
[0190] Lactic acid is water-soluble, and a 10% lactic acid solution can stimulate the keratinocytes and Langerhans cells of the skin to produce inflammatory response-related mediators, thereby producing related symptoms such as burning, stinging, and itching. This experiment is used to evaluate the sensitivity of the facial skin of the subjects, which can reflect the skin barrier function to a certain extent. As can be seen from Table 5, the cumulative score of the subject before using the product was 5 points, indicating that the subject's skin was sensitive. After using the composition of Examples 1-11 for 28 days, the cumulative score of the consumer's stinging sensation was 0, indicating that the composition of the present invention has an excellent soothing effect. After using the comparative example, the cumulative score of the stinging sensation was 4. Although it has decreased, it has not produced a soothing effect on sensitive skin, indicating that the barrier function of the comparative example has not changed significantly.
[0191] 1.5 Stability test
[0192] Test method: Use accelerated test for observation, place the samples in instruments at a certain temperature (-18℃±2℃, 4℃±2℃, 25℃±2℃, 40℃±2℃, 45℃±2℃), store them for 3 months away from direct sunlight, and inspect the samples.
[0193] The stability test results are as follows:
[0194] Table 6 Stability at different temperatures of Example 5, Comparative Examples 7-8 and Comparative Example 10
[0195]
[0196]
[0197] As can be seen from Table 6, Example 5 can remain stable for 3 months under different temperature conditions, and the product remains transparent, indicating that the composition of the present invention has good stability and can ensure product stability without the use of a suspending agent for suspension. In Comparative Examples 7-8, step 2 is not insulated and left to stand or the insulated and left to stand time is short, and the thickener in the system is still partially agglomerated. As time goes on and the temperature rises, the agglomerated part separates from the system, and a stratification problem occurs. Comparative Example 10 has white particles precipitated at all temperatures. The main reason is that ceramide 3 belongs to aliphatic amides, which are insoluble in water, resulting in its inability to dissolve in the aqueous formulation and cannot be used, and cannot exert its efficacy.
[0198] 1.6 Evaluation of spreadability
[0199] Table 7 Subjective evaluation of smearing of Example 5 and Comparative Examples 7-8
[0200] Example Initial application Mid to late stage of application Example 5 Easy to spread Apply evenly and smoothly Comparative Example 7 Slightly poor spreadability There is a sense of resistance when applying Comparative Example 8 Poor spreading The paint has a noticeable grainy feel
[0201] As can be seen from Table 7, compared with Example 5, whether in the early or middle and late stages of application, Comparative Examples 7-8 have differences in the application process. The main reason is that, compared with the embodiments, Comparative Examples 7-8 are not kept warm or the time of keeping warm is too short, resulting in the presence of incompletely swollen hydrolyzed sclerotium gum and xanthan gum in the system, but this incompletely swollen state is not easy to be found during the product preparation process, which ultimately affects the product quality and the consumer experience. It also further illustrates that the composition preparation method of the present invention can obtain a texture that is easier to apply and can effectively promote the uniform release of the efficacy of the composition.
[0202] 1.7 Safety Evaluation
[0203] Test method: Select a qualified patch tester and place 25 μL of the sample of Example 5 in the patch tester using the closed patch test method. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standard in the "Technical Specification for Safety of Cosmetics" (2015 edition).
[0204] Table 8 Skin reaction grading standards for skin occlusive patch test
[0205]
[0206] Table 9 Human skin patch test results
[0207]
[0208] The human skin patch test is suitable for detecting potential adverse reactions of cosmetic products to human skin. As can be seen from Table 9, in the 30 groups of effective patch data of Example 5, no abnormal skin reaction occurred within 48 hours after wiping off the patch, which shows that the composition of Example 5 is safe for application to human skin.
[0209] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent structural change made to the above embodiment according to the technical essence of the invention still falls within the protection scope of the technical solution of the present invention.
Claims
1. A composition having the effects of increasing skin lipids and soothing and repairing, characterized in that: Based on 100 parts by weight, the composition comprises: 0.2-0.4 parts by weight of hydrolyzed sclerotium gum, 0.05-0.2 parts by weight of xanthan gum, 3-8 parts by weight of glycerin, 0.05-0.2 parts by weight of sodium hyaluronate, 0.01-0.05 parts by weight of wheat seed extract, 0.005-0.01 parts by weight of flaxseed extract, 0.1-1 parts by weight of lactic acid bacteria fermentation extract, 0-0.01 parts by weight of mussel extract, 0.2-4 parts by weight of butylene glycol, 0.4-1 parts by weight of 1,2-hexanediol, 0.1-0.4 parts by weight of p-hydroxyacetophenone, 0.02-0.08 parts by weight of caprylhydroxamic acid, 0.005-0.02 parts by weight of ethylhexylglycerin, and the remainder is water.
2. The composition having the effects of increasing skin lipids and soothing and repairing skin according to claim 1, characterized in that: The mass ratio of the wheat seed extract to the flax seed extract is (1-10):
1.
3. The composition having the effects of increasing skin lipids and soothing and repairing skin according to claim 1, characterized in that: The mass ratio of the hydrolyzed sclerotium gum to the xanthan gum is (1-8):
1.
4. The composition having the effects of increasing skin lipids and soothing and repairing skin according to claim 1, characterized in that: The preparation method of the wheat seed extract comprises: washing and crushing the wheat seeds into 45-60 meshes, then adding the seeds into 20-45 times water and soaking them for 2h-3h, ultrasonicating them in a constant temperature water bath for 20min-35min, stirring and extracting them for 1h-2h, collecting the supernatant, adding an ethanol solution with a concentration of 50%-60% to the collected supernatant, stirring and extracting them for 2-3 times at room temperature, each extraction time being 1h-2h, separating the precipitate by centrifugation, and vacuum freeze-drying the separated precipitate to obtain the wheat seed extract.
5. The composition having the effects of increasing skin lipids and soothing and repairing skin according to claim 4, characterized in that: The temperature of the constant temperature water bath ultrasound is 45°C to 55°C.
6. The composition having the effects of increasing skin lipids and soothing and repairing skin according to claim 1, characterized in that: The DOPA content of the mussel extract is not less than 0.3%.
7. A method for preparing the composition having the effects of increasing skin lipids and soothing and repairing skin as claimed in claim 1, characterized in that: include: Step 1: Preprocessing, including: Step 101, placing hydrolyzed sclerotium gum, xanthan gum and sodium hyaluronate in glycerol, stirring to make them dispersed evenly, to obtain a reserve solution 1; Step 102, adding wheat seed extract to 50° C. to 55° C. water, stirring evenly, and when the temperature drops below 45° C., adding flax seed extract, stirring evenly, to obtain a reserve solution 2; Step 103, adding the mussel extract and the lactic acid bacteria fermentation extract into the water, stirring evenly, to obtain a reserve solution 3; Step 2, add the reserve solution 1 to the water at 70℃~75℃, set the stirring speed to 700r / min~900r / min, stir evenly and keep warm to let it swell until it is completely transparent, and stir and mix evenly; Step 3: When the temperature of the material in step 2 drops to 40°C to 45°C, add reserve solution 2, reserve solution 3, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid and ethylhexylglycerin, stir until mixed, filter, and obtain a composition with the effects of increasing skin lipids and soothing and repairing.
8. The method according to claim 5, characterized in that The heat preservation and standing time in step 2 is 20min to 30min.