A probiotic composition and its preparation method and application

Abstract

The invention belongs to the technical field of probiotics, and the present invention specifically discloses a probiotic composition and its preparation method and application, including the following steps: Hawthorn powder, carob powder, elderberry powder are mixed to obtain a mixture; The mixture is added to a liquid culture medium to obtain a fermentation medium; Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid are inoculated into a fermentation medium, and fermentation is performed to obtain a fermentation product; Fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharides, inulin, and β-cyclodextrin are mixed to obtain a probiotic composition. The present invention mixes hawthorn powder, carob powder, and elderberry powder, and as a fermentation medium, inoculates Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid, and fermentation and culture are performed, and then the fermentation product is mixed with Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharides, inulin, and β-cyclodextrin, and a probiotic composition with excellent antioxidant and anti-inflammatory effects is obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of probiotics, and in particular to a probiotic composition and a preparation method and application thereof. Background Art

[0002] Although traditional antioxidant and anti-inflammatory drugs can alleviate oxidative stress and inflammation to a certain extent, long-term use may have side effects. Therefore, it is particularly important to develop a safe and effective antioxidant and anti-inflammatory product.

[0003] Probiotics, also known as "friendly bacteria" or "beneficial bacteria", are a type of active microorganisms that are beneficial to human health. They mainly exist in the human intestine and play an important role in maintaining human health by regulating the balance of intestinal microecology. Probiotics mainly come from fermented foods, supplements and the natural environment. Common fermented foods include yogurt, kimchi, sauerkraut, miso, natto, etc. These foods produce a large number of probiotics during the fermentation process. Supplements are health products or medicines with probiotics as the main ingredient, which are taken orally. There are many types of probiotics, including Lactobacillus, Bifidobacterium, Streptococcus, Saccharomyces boulardii, etc. Different types of probiotics may also have different functions and effects in the human body. Probiotics play a variety of physiological functions in the human body, including: Regulating the balance of intestinal flora: Maintaining the balance of intestinal microecology by competitively inhibiting the growth of harmful bacteria. Enhance immunity: Stimulate the intestinal immune system and improve the body's resistance to pathogens. Promote nutrient absorption: Decompose complex components in food, such as cellulose, to promote the absorption and utilization of nutrients. Relieve constipation and diarrhea: Improve intestinal peristalsis, promote defecation, and help relieve diarrhea symptoms. Lower cholesterol: Certain probiotics can lower blood cholesterol levels and prevent cardiovascular disease.

[0004] In view of this, this application is filed. Summary of the invention

[0005] The invention provides a probiotic composition and a preparation method and application thereof. The probiotic composition has excellent anti-oxidation and anti-inflammatory effects.

[0006] The present invention solves the technical problem by adopting the following technical solutions: A method for preparing a probiotic composition comprises the following steps: (1) Mix hawthorn powder, carob powder, and elderberry powder to obtain a mixture; (2) adding the mixture to a liquid culture medium to obtain a fermentation medium; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, fermenting, and obtaining a fermentation product; (4) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0007] The present invention creatively mixes hawthorn powder, carob powder and elderberry powder as a fermentation medium, inoculates Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid for fermentation and culture, and then evenly mixes the fermentation product with Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharides, inulin and β-cyclodextrin to obtain a probiotic composition with excellent antioxidant and anti-inflammatory effects.

[0008] Hawthorn powder: Hawthorn is rich in antioxidant ingredients such as flavonoids (such as hawthorn flavonoids), organic acids (such as chlorogenic acid), and vitamin C. Hawthorn flavonoids and vitamin C have strong antioxidant effects, which can scavenge free radicals, slow down oxidative damage, and protect cells from peroxides and free radicals. The organic acids and flavonoids in hawthorn help inhibit the release of inflammatory mediators and reduce inflammatory responses. They reduce the production of cytokines such as tumor necrosis factor (TNF-α) and interleukins by inhibiting the NF-κB pathway, etc., and relieve inflammation.

[0009] Carob powder: Carob is rich in polyphenolic compounds, plant protein, dietary fiber and a small amount of natural sugars. The polyphenolic compounds in carob (such as ellagic acid and caffeic acid) have strong antioxidant capacity, which can remove free radicals in the body, reduce oxidative stress and slow down the aging process. The polyphenolic compounds in carob also have anti-inflammatory effects, which can regulate immune response, inhibit the excessive release of inflammatory factors, reduce tissue damage and relieve inflammation caused by oxidative stress.

[0010] Elderberry powder: Elderberry is rich in anthocyanins, vitamin C and flavonoids. Elderberry is rich in anthocyanins, which have significant antioxidant activity, can inhibit free radicals, protect cells from oxidative damage, promote skin health and slow down aging. Natural compounds in elderberry (such as anthocyanins and flavonoids) can inhibit inflammatory pathways in cells, such as regulating the NF-κB signaling pathway, reducing inflammatory responses, and relieving skin redness and allergies caused by inflammation.

[0011] The hawthorn powder, carob powder and elderberry powder are combined. The antioxidant ingredients (flavonoids, polyphenols, anthocyanins, vitamin C, etc.) in hawthorn powder, carob powder and elderberry powder have different mechanisms of action and can play an antioxidant role at different levels. Their synergistic effect can not only scavenge free radicals, but also enhance the antioxidant effect in a complementary way. The natural ingredients in these three plant powders inhibit inflammatory responses through multiple pathways. After mixing and fermentation, microorganisms will break down macromolecules in plants, such as fiber and protein, to form small molecules, thereby enhancing the bioavailability and absorption rate of these ingredients, making them easier to be absorbed by the skin or body. The fermentation process can transform the active ingredients in these plant powders through the action of microorganisms, enhancing their antioxidant and anti-inflammatory effects.

[0012] As a preferred embodiment of the present invention, the mass ratio of the hawthorn powder, carob powder and elderberry powder is 1: (0.5-1.5): (0.5-1.5).

[0013] As a preferred embodiment of the present invention, the average particle size of the hawthorn powder is 50-400 mesh; The average particle size of the carob powder is 50-400 mesh; The average particle size of the elderberry powder is 50-400 meshes.

[0014] As a preferred embodiment of the present invention, the liquid culture medium comprises the following components in parts by mass: 2 to 6 parts of beef extract, 4 to 10 parts of yeast extract powder, 15 to 20 parts of glucose, 4 to 10 parts of lactose, 4 to 10 parts of peptone, 0.02 to 0.05 parts of magnesium sulfate heptahydrate, 0.02 to 0.06 parts of dipotassium hydrogen phosphate, 0.02 to 0.05 parts of diammonium citrate, 0.5 to 2 parts of Tween-80, 0.01 to 0.04 parts of sodium bicarbonate, and 1000 parts of water.

[0015] As a preferred embodiment of the present invention, the solid-to-liquid ratio of the mixture and the liquid culture medium is (10-20) mg:1 mL.

[0016] As a preferred embodiment of the present invention, the concentration of Lactobacillus acidophilus in the Lactobacillus acidophilus seed solution is 10 8 ~10 10 cfu / mL; The concentration of Lactobacillus rhamnosus in the Lactobacillus rhamnosus seed solution is 10 7 ~10 9 cfu / mL.

[0017] As a preferred embodiment of the present invention, the inoculation amount of the Lactobacillus acidophilus seed solution is 1-2wt%; The inoculation amount of the Lactobacillus rhamnosus seed solution is 1-2wt%.

[0018] As a preferred embodiment of the present invention, the mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharides, inulin and β-cyclodextrin is 100:(1-5):(1-5):(20-50):(20-50):(5-20).

[0019] The present invention also provides a probiotic composition, which is prepared by the above-mentioned preparation method.

[0020] The present invention also provides an application of a probiotic composition in the preparation of medicines, foods or daily necessities with anti-oxidation and / or anti-inflammatory effects.

[0021] The beneficial effects of the present invention are as follows: (1) The present invention creatively mixes hawthorn powder, carob powder and elderberry powder as a fermentation medium, inoculates Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid for fermentation and culture, and then evenly mixes the fermentation product with Bifidobacterium bifidum, Bifidobacterium animalis, oligoxylose, inulin and β-cyclodextrin to obtain a probiotic composition with excellent antioxidant and anti-inflammatory effects. (2) The present invention combines hawthorn powder, carob powder and elderberry powder. The antioxidant components (flavonoids, polyphenols, anthocyanins, vitamin C, etc.) in hawthorn powder, carob powder and elderberry powder have different mechanisms of action and can exert antioxidant effects at different levels. Their synergistic effect can not only remove free radicals, but also enhance the antioxidant effect in a complementary manner. The natural ingredients in these three plant powders inhibit inflammatory responses through multiple pathways. After mixing and fermentation, microorganisms will decompose macromolecules in plants, such as fiber and protein, to form small molecules, thereby enhancing the bioavailability and absorption rate of these components, making them easier to be absorbed by the skin or the body. The fermentation process can transform the active ingredients in these plant powders through the action of microorganisms, enhancing their antioxidant and anti-inflammatory effects. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0024] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0025] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0026] The reagents or instruments used in the present invention without indicating the manufacturer are all conventional products that can be obtained commercially. The raw materials used in the comparative examples and the raw materials used in the parallel experiments of the embodiments are the same commercially available products unless otherwise specified.

[0027] The Lactobacillus acidophilus in the examples and comparative examples of the present invention is LA1063; the Bifidobacterium longum is BL986; and the Lactobacillus rhamnosus is LRH10.

[0028] The strain of Bifidobacterium bifidum is BB14; the strain of Bifidobacterium animalis is BAL06.

[0029] Example 1 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0030] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0031] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0032] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0033] Example 2 A method for preparing a probiotic composition comprises the following steps: (1) 100 mesh hawthorn powder, 100 mesh carob powder, and 100 mesh elderberry powder are mixed in a mass ratio of 1:1.5:0.5 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0034] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0035] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0036] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0037] Example 3 A method for preparing a probiotic composition comprises the following steps: (1) mixing 100 mesh hawthorn powder, 100 mesh carob powder, and 100 mesh elderberry powder in a mass ratio of 1:0.5:1.5 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0038] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0039] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0040] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0041] Example 4 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0042] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed liquid is 1wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 2wt%.

[0043] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0044] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0045] Example 5 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0046] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed liquid is 2wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1wt%.

[0047] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0048] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0049] Example 6 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 2 parts of beef extract, 10 parts of yeast extract powder, 15 parts of glucose, 10 parts of lactose, 4 parts of peptone, 0.05 parts of magnesium sulfate heptahydrate, 0.02 parts of dipotassium hydrogen phosphate, 0.05 parts of diammonium citrate, 0.5 parts of Tween-80, 0.04 parts of sodium bicarbonate, and 1000 parts of water.

[0050] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0051] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0052] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0053] Example 7 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 6 parts of beef extract, 4 parts of yeast extract powder, 20 parts of glucose, 4 parts of lactose, 10 parts of peptone, 0.02 parts of magnesium sulfate heptahydrate, 0.06 parts of dipotassium hydrogen phosphate, 0.02 parts of diammonium citrate, 2 parts of Tween-80, 0.01 parts of sodium bicarbonate, and 1000 parts of water.

[0054] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0055] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0056] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0057] Comparative Example 1 A method for preparing a probiotic composition comprises the following steps: (1) adding 100-mesh hawthorn powder into a liquid culture medium and mixing well to obtain a fermentation medium; The solid-liquid ratio of the hawthorn powder and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0058] (2) Inoculate Lactobacillus acidophilus into Gould's medium and activate and culture at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0059] (4) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0060] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0061] Comparative Example 2 A method for preparing a probiotic composition comprises the following steps: (1) adding 100-mesh carob powder to a liquid culture medium and mixing well to obtain a fermentation medium; The solid-to-liquid ratio of the carob powder and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0062] (2) Inoculate Lactobacillus acidophilus into Gould's medium and activate and culture at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0063] (4) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0064] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0065] Comparative Example 3 A method for preparing a probiotic composition comprises the following steps: (1) adding 100-mesh elderberry powder to a liquid culture medium and mixing well to obtain a fermentation medium; The solid-liquid ratio of the elderberry powder and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0066] (2) Inoculate Lactobacillus acidophilus into Gould's medium and activate and culture at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0067] (4) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0068] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0069] Comparative Example 4 A method for preparing a probiotic composition comprises the following steps: (1) mixing 100-mesh hawthorn powder and 100-mesh carob powder in a mass ratio of 1:1.2 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0070] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 109 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0071] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0072] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0073] Comparative Example 5 A method for preparing a probiotic composition comprises the following steps: (1) mixing 100 mesh hawthorn powder and 100 mesh elderberry powder in a mass ratio of 1:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0074] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0075] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0076] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0077] Comparative Example 6 A method for preparing a probiotic composition comprises the following steps: (1) mixing 100-mesh carob powder and 100-mesh elderberry powder in a mass ratio of 1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0078] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0079] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0080] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0081] Comparative Example 7 A method for preparing a probiotic composition comprises the following steps: (1) 100-mesh grape skin powder, 100-mesh carob powder, and 100-mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0082] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0083] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0084] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0085] Comparative Example 8 A method for preparing a probiotic composition comprises the following steps: (1) 100 mesh apple peel powder, 100 mesh carob powder, and 100 mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.2:0.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0086] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0087] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0088] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0089] Comparative Example 9 A method for preparing a probiotic composition comprises the following steps: (1) 100 mesh hawthorn powder, 100 mesh carob powder, and 100 mesh elderberry powder are uniformly mixed in a mass ratio of 1:0.2:1.8 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0090] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0091] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0092] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0093] Comparative Example 10 A method for preparing a probiotic composition comprises the following steps: (1) 100 mesh hawthorn powder, 100 mesh carob powder, and 100 mesh elderberry powder are uniformly mixed in a mass ratio of 1:1.8:0.2 to obtain a mixture; (2) adding the mixture to a liquid culture medium and mixing them evenly to obtain a fermentation medium; The solid-to-liquid ratio of the mixture and the liquid culture medium is 18 mg:1 mL; The liquid culture medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 parts of magnesium sulfate heptahydrate, 0.05 parts of dipotassium hydrogen phosphate, 0.04 parts of diammonium citrate, 1.5 parts of Tween-80, 0.03 parts of sodium bicarbonate, and 1000 parts of water.

[0094] (3) Lactobacillus acidophilus was inoculated into Gould's medium and activated and cultured at 60 r / min and 37°C for 14 h to obtain a Lactobacillus acidophilus concentration of 10 9 cfu / m of Lactobacillus acidophilus seed solution; Lactobacillus rhamnosus was inoculated into Gould's medium and activated and cultured for 14 h at 60 r / min and 37 °C to obtain a Lactobacillus rhamnosus concentration of 10 9 cfu / m of Lactobacillus rhamnosus seed solution; (4) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, and fermenting at 36° C. for 48 hours to obtain a fermentation product; The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5wt%.

[0095] (5) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

[0096] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:3:2:40:30:10.

[0097] 1. Detection of hydroxyl radical HO scavenging ability Antioxidant performance test: add 1mL salicylic acid-ethanol solution (5mmol / L), 1mL FeSO4 solution (5mmol / L), 1mL H2O2 solution (3mmol / L) and 1mg probiotic composition to a test tube and mix well, then dilute to 10mL with double distilled water, bath at 37℃ for 15min, centrifuge at 4℃, 6000rpm for 10min, take the supernatant and measure the absorbance at 510nm (with double distilled water as reference). HO· scavenging rate (%) = [(Ao-As) / Ao] × 100% (Ao is the OD value without sample; As is the OD value with sample), the higher the HO· scavenging rate, the stronger the scavenging effect on active oxygen, that is, the stronger the antioxidant capacity. The test results are shown in Table 1.

[0098] 2. Anti-inflammatory experiment: HaCaT (human keratinocytes) were cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin at 37°C, 5% CO2, and 98% humidity. Cells in logarithmic growth phase were selected and inoculated in 96-well culture plates. After culturing for 24 hours, the cells were completely attached to the wall. The culture medium was replaced with RPMI1640 containing the probiotic composition of the embodiment and the comparative example (final concentration 0.6 mg / mL), and the cells were incubated for 12 hours at 30 mJ / cm 2The cells were irradiated with a dose of UVB at a distance of 15 cm. After irradiation, the cell culture supernatant was collected and the concentration of IL-1α in the supernatant was detected using a human ELISA kit. Each experiment was repeated three times, and the average value was taken as the final result. The test results are shown in Table 1.

[0099] Table 1

[0100] It can be seen from Table 1 that the probiotic composition of the present invention has excellent anti-oxidation and anti-inflammatory effects.

[0101] By comparing Example 1 with Comparative Examples 1 to 8, it can be seen that the hawthorn, carob powder and elderberry powder described in the present invention have significant synergistic effects in anti-oxidation and anti-inflammatory aspects. Under the joint action of the three, the anti-oxidation and anti-inflammatory effects are significantly improved. The lack of any one of them or the use of other ingredients for replacement will lead to a significant decrease in the anti-oxidation and anti-inflammatory effects.

[0102] By comparing Example 1 with Comparative Examples 9-10, it can be seen that the present invention significantly improves the antioxidant and anti-inflammatory effects by controlling the mass ratio of hawthorn, carob powder and elderberry powder to 1: (0.5-1.5): (0.5-1.5), and controlling the mass ratio of the three within the scope of the present invention has a more ideal synergistic effect.

[0103] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a probiotic composition, characterized in that: The following steps are involved: (1) Mix hawthorn powder, carob powder, and elderberry powder to obtain a mixture; (2) adding the mixture to a liquid culture medium to obtain a fermentation medium; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, fermenting, and obtaining a fermentation product; (4) The fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and β-cyclodextrin are uniformly mixed to obtain a probiotic composition.

2. The method for preparing the probiotic composition according to claim 1, characterized in that: The mass ratio of the hawthorn powder, carob powder and elderberry powder is 1:(0.5-1.5):(0.5-1.5).

3. The method for preparing the probiotic composition according to claim 1, characterized in that: The average particle size of the hawthorn powder is 50-400 mesh; The average particle size of the carob powder is 50-400 mesh; The average particle size of the elderberry powder is 50-400 meshes.

4. The method for preparing the probiotic composition according to claim 1, characterized in that: The liquid culture medium comprises the following components in parts by mass: 2-6 parts of beef extract, 4-10 parts of yeast extract powder, 15-20 parts of glucose, 4-10 parts of lactose, 4-10 parts of peptone, 0.02-0.05 parts of magnesium sulfate heptahydrate, 0.02-0.06 parts of dipotassium hydrogen phosphate, 0.02-0.05 parts of diammonium citrate, 0.5-2 parts of Tween-80, 0.01-0.04 parts of sodium bicarbonate, and 1000 parts of water.

5. The method for preparing the probiotic composition according to claim 1, characterized in that: The solid-to-liquid ratio of the mixture and the liquid culture medium is (10-20) mg:1 mL.

6. The method for preparing the probiotic composition according to claim 1, characterized in that: The concentration of Lactobacillus acidophilus in the Lactobacillus acidophilus seed solution is 10 8 ~10 10 cfu / mL; The concentration of Lactobacillus rhamnosus in the Lactobacillus rhamnosus seed solution is 10 7 ~10 9 cfu / mL.

7. The method for preparing the probiotic composition according to claim 1, characterized in that: The inoculation amount of the Lactobacillus acidophilus seed solution is 1-2wt%; The inoculation amount of the Lactobacillus rhamnosus seed solution is 1-2wt%.

8. The method for preparing the probiotic composition according to claim 1, characterized in that: The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:(1-5):(1-5):(20-50):(20-50):(5-20).

9. A probiotic composition, characterized in that The method is prepared by any one of claims 1 to 8.

10. Use of the probiotic composition according to any one of claims 1 to 8 in the preparation of medicines, foods or daily necessities having antioxidant and / or anti-inflammatory effects.

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