A probiotic composition, its preparation method and applications

Abstract

The present invention belongs to the technical field of probiotics. Specifically, the present invention discloses a probiotic composition, its preparation method and application, which include the following steps: Mix hawthorn powder, carob powder, and elderberry powder evenly to obtain a mixture; Add the mixture to a liquid culture medium to obtain a fermentation medium; Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium and ferment to obtain a fermentation product; Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition. The present invention mixes hawthorn powder, carob powder, and elderberry powder as a fermentation medium, inoculates the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid, and performs fermentation culture. Then, the fermentation product is mixed evenly with Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin to obtain a probiotic composition with excellent antioxidant and anti-inflammatory effects.

Description

Technical Field

[0001] The present invention relates to the technical field of probiotics, and particularly relates to a probiotic composition, a preparation method thereof, and an application thereof. Background Art

[0002] Although traditional antioxidant and anti-inflammatory drugs can alleviate oxidative stress and inflammation problems to a certain extent, long-term use may have side effects. Therefore, it is particularly important to develop a safe and effective antioxidant and anti-inflammatory product.

[0003] Probiotics, also known as "friendly bacteria" or "beneficial bacteria", are a type of active microorganisms beneficial to human health. They mainly exist in the human intestine and play an important role in maintaining human health by regulating the intestinal microecological balance. Probiotics mainly come from fermented foods, supplements, and the natural environment. Common fermented foods include yogurt, pickles, sauerkraut, miso, natto, etc. A large number of probiotics are produced during the fermentation process of these foods. Supplements are health products or drugs mainly composed of probiotics and are ingested orally. There are many types of probiotics, including Lactobacillus, Bifidobacterium, Streptococcus, Saccharomyces boulardii, etc. Different types of probiotics may have different functions and effects in the human body. Probiotics play a variety of physiological functions in the human body, including: regulating the balance of intestinal flora: maintaining the intestinal microecological balance by competitively inhibiting the growth of harmful bacteria. Enhancing immunity: stimulating the intestinal immune system and improving the body's resistance to pathogens. Promoting nutrient absorption: decomposing complex components in food, such as cellulose, and promoting the absorption and utilization of nutrients. Relieving constipation and diarrhea: improving intestinal peristalsis, promoting defecation, and at the same time helping to relieve diarrhea symptoms. Lowering cholesterol: certain probiotics can lower the cholesterol level in the blood and prevent cardiovascular diseases.

[0004] In view of this, the present application is proposed. Summary of the Invention

[0005] The present invention provides a probiotic composition, a preparation method thereof, and an application thereof, and the probiotic composition has excellent antioxidant and anti-inflammatory effects.

[0006] The present invention solves its technical problems by adopting the following technical solutions:

[0007] A preparation method of a probiotic composition, comprising the following steps:

[0008] (1) Mix hawthorn powder, carob powder, and elderberry powder evenly to obtain a mixture;

[0009] (2) Add the mixture to a liquid culture medium to obtain a fermentation culture medium;

[0010] (3) Inoculate the Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into the fermentation medium, and ferment to obtain a fermentation product;

[0011] (4) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0012] In the present invention, hawthorn powder, carob powder, and elderberry powder are creatively mixed as the fermentation medium, inoculated with Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid, and subjected to fermentation culture. Then, the fermentation product is mixed evenly with Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin to obtain a probiotic composition with excellent antioxidant and anti-inflammatory effects.

[0013] Hawthorn powder: Hawthorn is rich in antioxidant components such as flavonoids (such as hawthorn flavonoids), organic acids (such as chlorogenic acid), and vitamin C. The flavonoids and vitamin C in hawthorn have strong antioxidant effects, can scavenge free radicals, slow down oxidative damage, and protect cells from peroxides and free radicals. The organic acids and flavonoids in hawthorn help inhibit the release of inflammatory mediators and reduce the inflammatory response. They reduce the production of cytokines such as tumor necrosis factor (TNF-α) and interleukin by inhibiting the NF-κB pathway, etc., and relieve inflammation.

[0014] Carob powder: Carob is rich in polyphenolic compounds, plant proteins, dietary fiber, and a small amount of natural sugars. The polyphenolic compounds (such as ellagic acid and caffeic acid) in carob have strong antioxidant capabilities, can scavenge free radicals in the body, reduce oxidative stress, and slow down the aging process. The polyphenolic compounds in carob also have anti-inflammatory effects, can regulate the immune response, inhibit the excessive release of inflammatory factors, reduce tissue damage, and relieve inflammation caused by oxidative stress.

[0015] Elderberry powder: Elderberry is rich in anthocyanins, vitamin C, and flavonoids. Elderberry is rich in anthocyanins, and anthocyanins have significant antioxidant activity, can inhibit free radicals, protect cells from oxidative damage, promote skin health, and slow down aging. The natural compounds (such as anthocyanins and flavonoids) in elderberry can inhibit the inflammatory pathways in cells, such as by regulating the NF-κB signaling pathway, reducing the inflammatory response, and relieving skin redness and allergies caused by inflammation.

[0016] Combining hawthorn powder, carob powder and elderberry powder, the antioxidant components (such as flavonoids, polyphenols, anthocyanins, vitamin C, etc.) in hawthorn powder, carob powder and elderberry powder have different action mechanisms and can play antioxidant roles at different levels. Their synergistic effect can not only scavenge free radicals, but also enhance the antioxidant effect in a complementary way. The natural components in these three plant powders inhibit the inflammatory response through various ways. After mixing and fermentation, microorganisms will decompose macromolecular substances in plants, such as fibers and proteins, into small molecular substances, thereby enhancing the bioavailability and absorption rate of these components and making them more easily absorbed by the skin or the body. The fermentation process can transform the active components in these plant powders through the action of microorganisms and enhance their antioxidant and anti-inflammatory effects.

[0017] As a preferred embodiment of the present invention, the mass ratio of the hawthorn powder, carob powder, and elderberry powder is 1:(0.5 - 1.5):(0.5 - 1.5).

[0018] As a preferred embodiment of the present invention, the average particle size of the hawthorn powder is 50 - 400 mesh;

[0019] The average particle size of the carob powder is 50 - 400 mesh;

[0020] The average particle size of the elderberry powder is 50 - 400 mesh.

[0021] As a preferred embodiment of the present invention, the liquid medium comprises the following components in parts by mass: 2 - 6 parts of beef extract, 4 - 10 parts of yeast extract powder, 15 - 20 parts of glucose, 4 - 10 parts of lactose, 4 - 10 parts of peptone, 0.02 - 0.05 parts of magnesium sulfate heptahydrate, 0.02 - 0.06 parts of dipotassium hydrogen phosphate, 0.02 - 0.05 parts of diammonium citrate, 0.5 - 2 parts of Tween - 80, 0.01 - 0.04 parts of sodium bicarbonate, and 1000 parts of water.

[0022] As a preferred embodiment of the present invention, the solid - liquid ratio of the mixture to the liquid medium is (10 - 20) mg:1 mL.

[0023] As a preferred embodiment of the present invention, the concentration of Lactobacillus acidophilus in the Lactobacillus acidophilus seed liquid is 10 8 ~10 10 cfu / mL;

[0024] The concentration of Lactobacillus rhamnosus in the Lactobacillus rhamnosus seed liquid is 10 7 ~10 9 cfu / mL.

[0025] As a preferred embodiment of the present invention, the inoculation amount of the Lactobacillus acidophilus seed liquid is 1 - 2 wt%;

[0026] The inoculation amount of the Lactobacillus rhamnosus seed liquid is 1-2 wt%.

[0027] As a preferred embodiment of the present invention, the mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100: (1-5): (1-5): (20-50): (20-50): (5-20).

[0028] The present invention also provides a probiotic composition prepared by the above-mentioned preparation method.

[0029] The present invention also provides an application of the probiotic composition in the preparation of a drug, food, or daily necessities having antioxidant and / or anti-inflammatory effects.

[0030] Advantages of the present invention: (1) The present invention creatively mixes hawthorn powder, carob powder, and elderberry powder as a fermentation medium, inoculates Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid, and performs fermentation culture. Then, the fermentation product is mixed evenly with Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin to obtain a probiotic composition with excellent antioxidant and anti-inflammatory effects. (2) The present invention combines hawthorn powder, carob powder, and elderberry powder. The antioxidant components (flavonoids, polyphenols, anthocyanins, vitamin C, etc.) in hawthorn powder, carob powder, and elderberry powder have different action mechanisms and can play antioxidant roles at different levels. Their synergistic effect can not only scavenge free radicals but also enhance the antioxidant effect in a complementary manner. The natural components in these three plant powders inhibit the inflammatory response through various pathways. After mixing and fermentation, microorganisms will decompose macromolecular substances in the plants, such as fibers and proteins, into small molecular substances, thereby enhancing the bioavailability and absorption rate of these components and making them more easily absorbed by the skin or in the body. The fermentation process can transform the active components in these plant powders through the action of microorganisms, enhancing their antioxidant and anti-inflammatory effects. Detailed Embodiments

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0032] In the present invention, among the technically characterized described in an open-ended manner, there are included closed technical solutions composed of the listed features, as well as open technical solutions containing the listed features.

[0033] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as every value between such minimum and maximum values. Further, when the range refers to integers, every integer between the minimum and maximum values of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0034] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0035] For the reagents or instruments used in the present invention, if the manufacturer is not specified, they are all conventional products that can be obtained through commercial purchase. The raw materials used in each comparative example and the raw materials used in the parallel experiments of each example are the same commercially available products unless otherwise specified.

[0036] The Lactobacillus acidophilus in the examples and comparative examples of the present invention is LA1063; the Bifidobacterium longum is BL986; the Lactobacillus rhamnosus is LRH10.

[0037] The Bifidobacterium bifidum is BB14; the Bifidobacterium animalis is BAL06.

[0038] Example 1

[0039] A method for preparing a probiotic composition, comprising the following steps:

[0040] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0041] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0042] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0043] The liquid medium comprises the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0044] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0045] Inoculate Lactobacillus rhamnosus into Gao's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0046] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0047] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0048] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0049] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0050] Example 2

[0051] A preparation method of a probiotic composition, comprising the following steps:

[0052] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.5:0.5 to obtain a mixture;

[0053] (2) Add the mixture to the liquid medium and mix evenly to obtain a fermentation medium;

[0054] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0055] The liquid medium comprises the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0056] (3) Inoculate Lactobacillus acidophilus into Gao's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0057] Inoculate Lactobacillus rhamnosus into Gao's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0058] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0059] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0060] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0061] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0062] Example 3

[0063] A method for preparing a probiotic composition, comprising the following steps:

[0064] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:0.5:1.5 to obtain a mixture;

[0065] (2) Add the mixture to the liquid medium and mix evenly to obtain a fermentation medium;

[0066] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0067] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0068] (3) Inoculate Lactobacillus acidophilus into the Gao's medium, and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / m;

[0069] Inoculate Lactobacillus rhamnosus into the Gao's medium, and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / m;

[0070] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0071] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0072] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0073] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0074] Example 4

[0075] A preparation method of a probiotic composition, comprising the following steps:

[0076] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0077] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0078] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0079] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0080] (3) Inoculate Lactobacillus acidophilus into a Gause's medium and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / m;

[0081] Inoculate Lactobacillus rhamnosus into a Gause's medium and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / m;

[0082] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0083] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 2 wt%.

[0084] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0085] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0086] Example 5

[0087] A method for preparing a probiotic composition, comprising the following steps:

[0088] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0089] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0090] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0091] The liquid medium comprises the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0092] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / mL;

[0093] Inoculate Lactobacillus rhamnosus into Gause's medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / mL;

[0094] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0095] The inoculation amount of the Lactobacillus acidophilus seed solution is 2 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1 wt%.

[0096] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0097] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0098] Example 6

[0099] A method for preparing a probiotic composition, comprising the following steps:

[0100] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to the mass ratio of 1:1.2:0.8 to obtain a mixture;

[0101] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0102] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0103] The liquid medium comprises the following components in parts by mass: 2 parts of beef extract, 10 parts of yeast extract powder, 15 parts of glucose, 10 parts of lactose, 4 parts of peptone, 0.05 part of magnesium sulfate heptahydrate, 0.02 part of dipotassium hydrogen phosphate, 0.05 part of diammonium citrate, 0.5 part of Tween-80, 0.04 part of sodium bicarbonate, and 1000 parts of water.

[0104] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0105] Inoculate Lactobacillus rhamnosus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0106] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0107] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0108] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0109] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0110] Example 7

[0111] A preparation method of a probiotic composition, comprising the following steps:

[0112] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0113] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0114] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0115] The liquid medium comprises the following components in parts by mass: 6 parts of beef extract, 4 parts of yeast extract powder, 20 parts of glucose, 4 parts of lactose, 10 parts of peptone, 0.02 part of magnesium sulfate heptahydrate, 0.06 part of dipotassium hydrogen phosphate, 0.02 part of diammonium citrate, 2 parts of Tween-80, 0.01 part of sodium bicarbonate, and 1000 parts of water.

[0116] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / mL;

[0117] Inoculate Lactobacillus rhamnosus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / mL;

[0118] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0119] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0120] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0121] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0122] Comparative Example 1

[0123] A preparation method of a probiotic composition, comprising the following steps:

[0124] (1) Add 100-mesh hawthorn powder to the liquid medium and mix evenly to obtain a fermentation medium;

[0125] The solid-liquid ratio of the hawthorn powder to the liquid medium is 18 mg: 1 mL;

[0126] The liquid medium includes the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0127] (2) Inoculate Lactobacillus acidophilus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0128] Inoculate Lactobacillus rhamnosus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0129] (3) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0130] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0131] (4) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0132] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100: 3: 2: 40: 30: 10.

[0133] Comparative Example 2

[0134] A method for preparing a probiotic composition, comprising the following steps:

[0135] (1) Add 100-mesh carob powder to the liquid medium and mix evenly to obtain a fermentation medium;

[0136] The solid-liquid ratio of the carob powder to the liquid medium is 18 mg: 1 mL;

[0137] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0138] (2) Inoculate Lactobacillus acidophilus into Gause's medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0139] Inoculate Lactobacillus rhamnosus into Gause's medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0140] (3) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0141] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0142] (4) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0143] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0144] Comparative Example 3

[0145] A method for preparing a probiotic composition, comprising the following steps:

[0146] (1) Add 100-mesh elderberry powder to the liquid medium, and mix evenly to obtain a fermentation medium;

[0147] The solid-liquid ratio of the elderberry powder to the liquid medium is 18 mg:1 mL;

[0148] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0149] (2) Inoculate Lactobacillus acidophilus into Czapek medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / mL;

[0150] Inoculate Lactobacillus rhamnosus into Czapek medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / mL;

[0151] (3) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0152] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0153] (4) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0154] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0155] Comparative Example 4

[0156] A method for preparing a probiotic composition, comprising the following steps:

[0157] (1) Mix 100-mesh hawthorn powder and 100-mesh carob powder evenly according to a mass ratio of 1:1.2 to obtain a mixture;

[0158] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0159] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0160] The liquid medium comprises the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0161] (3) Inoculate Lactobacillus acidophilus into Czapek medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / mL;

[0162] Inoculate Lactobacillus rhamnosus into Gao's medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0163] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0164] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0165] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0166] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0167] Comparative Example 5

[0168] A preparation method of a probiotic composition, comprising the following steps:

[0169] (1) Mix 100-mesh hawthorn powder and 100-mesh elderberry powder evenly according to a mass ratio of 1:0.8 to obtain a mixture;

[0170] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0171] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0172] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0173] (3) Inoculate Lactobacillus acidophilus into Gao's medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0174] Inoculate Lactobacillus rhamnosus into Gao's medium, and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0175] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0176] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0177] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0178] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0179] Comparative Example 6

[0180] A preparation method of a probiotic composition, comprising the following steps:

[0181] (1) Mix 100-mesh carob powder and 100-mesh elderberry powder evenly according to a mass ratio of 1.2:0.8 to obtain a mixture;

[0182] (2) Add the mixture to the liquid medium and mix evenly to obtain a fermentation medium;

[0183] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0184] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0185] (3) Inoculate Lactobacillus acidophilus into Gause's medium, and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / m;

[0186] Inoculate Lactobacillus rhamnosus into Gause's medium, and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / m;

[0187] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium, and ferment at 36 °C for 48 h to obtain a fermentation product;

[0188] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0189] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0190] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0191] Comparative Example 7

[0192] A preparation method of a probiotic composition includes the following steps:

[0193] (1) Mix 100-mesh grape skin powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0194] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0195] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0196] The liquid medium includes the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0197] (3) Inoculate Lactobacillus acidophilus into a Czapek medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / m;

[0198] Inoculate Lactobacillus rhamnosus into a Czapek medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / m;

[0199] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0200] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0201] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0202] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0203] Comparative Example 8

[0204] A method for preparing a probiotic composition, comprising the following steps:

[0205] (1) Mix 100-mesh apple peel powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.2:0.8 to obtain a mixture;

[0206] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0207] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0208] The liquid medium comprises the following components in parts by mass: 5 parts beef extract, 8 parts yeast extract powder, 18 parts glucose, 6 parts lactose, 8 parts peptone, 0.04 part magnesium sulfate heptahydrate, 0.05 part dipotassium hydrogen phosphate, 0.04 part diammonium citrate, 1.5 parts Tween-80, 0.03 part sodium bicarbonate, and 1000 parts water.

[0209] (3) Inoculate Lactobacillus acidophilus into a Gao's medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed solution with a concentration of 10 9 cfu / m;

[0210] Inoculate Lactobacillus rhamnosus into a Gao's medium and activate and culture it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed solution with a concentration of 10 9 cfu / m;

[0211] (4) Inoculate the Lactobacillus acidophilus seed solution and the Lactobacillus rhamnosus seed solution into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0212] The inoculation amount of the Lactobacillus acidophilus seed solution is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed solution is 1.5 wt%.

[0213] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0214] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0215] Comparative Example 9

[0216] A preparation method of a probiotic composition includes the following steps:

[0217] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to the mass ratio of 1:0.2:1.8 to obtain a mixture;

[0218] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0219] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0220] The liquid medium includes the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0221] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / mL;

[0222] Inoculate Lactobacillus rhamnosus into Gause's medium and activate it at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / mL;

[0223] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0224] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0225] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0226] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylooligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0227] Comparative Example 10

[0228] A preparation method of a probiotic composition, comprising the following steps:

[0229] (1) Mix 100-mesh hawthorn powder, 100-mesh carob powder, and 100-mesh elderberry powder evenly according to a mass ratio of 1:1.8:0.2 to obtain a mixture;

[0230] (2) Add the mixture to a liquid medium and mix evenly to obtain a fermentation medium;

[0231] The solid-liquid ratio of the mixture to the liquid medium is 18 mg:1 mL;

[0232] The liquid medium comprises the following components in parts by mass: 5 parts of beef extract, 8 parts of yeast extract powder, 18 parts of glucose, 6 parts of lactose, 8 parts of peptone, 0.04 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.04 part of diammonium citrate, 1.5 parts of Tween-80, 0.03 part of sodium bicarbonate, and 1000 parts of water.

[0233] (3) Inoculate Lactobacillus acidophilus into Gause's medium and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus acidophilus seed liquid with a concentration of 10 9 cfu / m;

[0234] Inoculate Lactobacillus rhamnosus into Gause's medium and activate and culture at 60 r / min and 37 °C for 14 h to obtain a Lactobacillus rhamnosus seed liquid with a concentration of 10 9 cfu / m;

[0235] (4) Inoculate the Lactobacillus acidophilus seed liquid and the Lactobacillus rhamnosus seed liquid into the fermentation medium and ferment at 36 °C for 48 h to obtain a fermentation product;

[0236] The inoculation amount of the Lactobacillus acidophilus seed liquid is 1.5 wt%; the inoculation amount of the Lactobacillus rhamnosus seed liquid is 1.5 wt%.

[0237] (5) Mix the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin evenly to obtain a probiotic composition.

[0238] The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin is 100:3:2:40:30:10.

[0239] 1. Detect the scavenging ability of hydroxyl radical HO·

[0240] Antioxidant performance test: Add 1 mL of salicylic acid - ethanol solution (5 mmol / L), 1 mL of FeSO 4 solution (5 mmol / L), 1 mL of H 2 O 2 solution (3 mmol / L) and 1 mg of the probiotic composition into a test tube and mix well. Then, make up the volume to 10 mL with double-distilled water, incubate in a water bath at 37 °C for 15 min, centrifuge at 4 °C and 6000 rpm for 10 min, and take the supernatant to measure the absorbance at 510 nm (using double-distilled water as the reference). The HO· scavenging rate (%) = [(Ao - As) / Ao] × 100% (Ao is the OD value without the sample; As is the OD value with the sample). The higher the HO· scavenging rate, the stronger the scavenging effect on reactive oxygen species, that is, the stronger the antioxidant ability. The test results are shown in Table 1.

[0241] 2. Anti-inflammatory experiment: HaCaT (human keratinocytes) are cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin under the conditions of 37 °C, 5% CO 2 saturation and 98% humidity. Logarithmic growth phase cells are selected and inoculated into a 96-well culture plate. After the cells are completely adherent after 24 h of culture, the medium is replaced with RPMI1640 containing the probiotic compositions of the examples and comparative examples (final concentration 0.6 mg / mL), and after incubation for 12 h, irradiate with UVB at a dose of 30 mJ / cm 2 at a irradiation distance of 15 cm. After irradiation, collect the cell culture supernatant and detect the concentration of IL-1α in the supernatant using a human ELISA kit. Each experiment is repeated three times, and the average value is taken as the final result. The test results are shown in Table 1.

[0242] Table 1

[0243]

[0244] It can be seen from Table 1 that the probiotic composition described in the present invention has excellent antioxidant and anti-inflammatory effects.

[0245] By comparing Example 1 with Comparative Examples 1 - 8, it can be seen that the hawthorn, carob powder, and elderberry powder described in the present invention have a significant synergistic effect in terms of antioxidant and anti-inflammatory properties. Under the combined action of the three, the antioxidant and anti-inflammatory effects are significantly improved. Lack of any one of them, or using other ingredients to replace, will lead to a significant decline in the antioxidant and anti-inflammatory effects.

[0246] By comparing Example 1 with Comparative Examples 9 - 10, it can be seen that by controlling the mass ratio of hawthorn, carob powder, and elderberry powder to be 1:(0.5 - 1.5):(0.5 - 1.5), the antioxidant and anti-inflammatory effects are significantly improved. Controlling the mass ratio of the three within the scope of the present invention has a more ideal synergistic effect.

[0247] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a probiotic composition, characterized in that: The following steps are involved: (1) Mix hawthorn powder, carob powder, and elderberry powder to obtain a mixture; (2) adding the mixture to a liquid culture medium to obtain a fermentation medium; (3) inoculating Lactobacillus acidophilus seed liquid and Lactobacillus rhamnosus seed liquid into a fermentation medium, fermenting, and obtaining a fermentation product; (4) mixing the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin, and β-cyclodextrin to obtain a probiotic composition; The mass ratio of the hawthorn powder, carob powder and elderberry powder is 1: (0.5-1.5): (0.5-1.5); The concentration of Lactobacillus acidophilus in the Lactobacillus acidophilus seed solution is 10 8 ~10 10 cfu / mL; The concentration of Lactobacillus rhamnosus in the Lactobacillus rhamnosus seed solution is 10 7 ~10 9 cfu / mL; The inoculation amount of the Lactobacillus acidophilus seed solution is 1-2wt%; The inoculation amount of the Lactobacillus rhamnosus seed solution is 1-2wt%.

2. The method for preparing the probiotic composition according to claim 1, characterized in that: The average particle size of the hawthorn powder is 50-400 mesh; The average particle size of the carob powder is 50-400 mesh; The average particle size of the elderberry powder is 50-400 meshes.

3. The method for preparing the probiotic composition according to claim 1, characterized in that: The liquid culture medium comprises the following components in parts by mass: 2-6 parts of beef extract, 4-10 parts of yeast extract powder, 15-20 parts of glucose, 4-10 parts of lactose, 4-10 parts of peptone, 0.02-0.05 parts of magnesium sulfate heptahydrate, 0.02-0.06 parts of dipotassium hydrogen phosphate, 0.02-0.05 parts of diammonium citrate, 0.5-2 parts of Tween-80, 0.01-0.04 parts of sodium bicarbonate, and 1000 parts of water.

4. The method for preparing the probiotic composition according to claim 1, characterized in that: The solid-to-liquid ratio of the mixture and the liquid culture medium is (10-20) mg:1 mL.

5. The method for preparing the probiotic composition according to claim 1, characterized in that: The mass ratio of the fermentation product, Bifidobacterium bifidum, Bifidobacterium animalis, xylo-oligosaccharide, inulin and beta-cyclodextrin is 100:(1-5):(1-5):(20-50):(20-50):(5-20).

6. A probiotic composition, characterized in that The method is prepared by any one of claims 1 to 5.

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