Traditional Chinese medicine extract for improving production performance of laying hens as well as preparation method and application of traditional Chinese medicine extract

Abstract

The invention belongs to the technical field of chicken feed, and particularly relates to a traditional Chinese medicine extract for improving the production performance of laying hens and a preparation method and application thereof. The invention provides a traditional Chinese medicine extract. The traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 80-150 parts of pueraria lobata leaves, 30-80 parts of pueraria lobata, 40-70 parts of gynura procumbens and 10-30 parts of houttuynia cordata. When the traditional Chinese medicine extract is prepared, two times of enzymolysis and squeezing processes are adopted, and high-temperature and high-pressure treatment is not needed in the whole extraction process, so that the production cost is effectively reduced, and meanwhile, heat-sensitive components are reserved. The prepared traditional Chinese medicine extract is added into feed for laying hens according to 1000-3000 mg / kg, the average egg weight of eggs of the laying hens can be increased, the color of yolk is increased, and the cholesterol content of the yolk is reduced; moreover, the contents of serine, alanine and total amino acid in egg yolk of 3000mg / kg of the traditional Chinese medicine extract are remarkably increased, the quality of the egg yolk is relatively good, and the traditional Chinese medicine extract has relatively great economic value and market prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of chicken feed, and particularly relates to a traditional Chinese medicine extract for improving the production performance of laying hens, and a preparation method and application thereof. Background Art

[0002] Eggs are an indispensable food in people's daily life. They contain a lot of vitamins, minerals and proteins. The yolk, which is the most nutritious part of eggs, accounts for about 30%-32% of the volume of the whole egg. The main components are 17.5% protein, 32.5% fat, rich vitamin A and vitamin D, and high iron, phosphorus, sulfur and calcium and other minerals. Therefore, the quality of the yolk determines the quality of the egg. How to improve the production performance of laying hens and the quality of eggs is the key to improving laying hen farming.

[0003] Chinese patent CN 114209734 B provides a laying hen feed, which uses dandelion, eucommia bark, dipsacus root, angelica root, lily of the valley and glossy privet fruit as main raw materials, which are first soaked in an alkaline solution, then adjusted to acidic, concentrated and dried to obtain a Chinese medicine composition, which can improve the egg production rate and eggshell strength of laying hens, reduce the egg breakage rate, and increase the CAT content in the serum of laying hens. This method requires the use of alkali for soaking treatment, has high production costs, and has the problem of solvent residues, and has not studied the improvement of the quality of egg yolk, an important component of eggs. Therefore, it is urgent to develop a new Chinese medicine extract to improve the quality of egg yolk. Summary of the invention

[0004] In view of the above problems, the present invention provides a Chinese herbal extract for improving the production performance of laying hens, and a preparation method and application thereof. By optimizing the formula and preparation method of the Chinese herbal extract, a Chinese herbal extract that can improve the quality of eggs and yolks is obtained. The preparation method of the Chinese herbal extract is simple, has no solvent residue, and has low production cost.

[0005] In order to solve the above technical problems of the present invention, the present invention provides the following technical solutions:

[0006] The first object of the present invention is to provide a Chinese medicinal extract for improving the production performance of laying hens. The raw material ratio of the Chinese medicinal extract is 80-150 parts of kudzu leaves, 30-80 parts of kudzu roots, 40-70 parts of chrysanthemum notoginseng, and 10-30 parts of houttuynia cordata.

[0007] Kudzu leaves are rich in various nutrients, such as protein, crude fiber, essential amino acids, etc. Among them, adenosine, aspartic acid, glutamic acid and other ingredients have a positive effect on the health and growth of livestock, and have good palatability; Kudzu root is rich in starch and a variety of functional ingredients, such as soy bean extract and genistein, which are beneficial to the growth and health of livestock. In addition, like kudzu leaves, it contains adenosine, aspartic acid and glutamic acid, and is a good source of feed; Chrysanthemum and Panax notoginseng clear away heat and detoxify, and Houttuynia cordata contains protein, vitamins, minerals and other ingredients, which can promote the growth and development of livestock and enhance immunity.

[0008] The inventors found that the Chinese medicine extract prepared by using 80-150 parts of kudzu leaves, 30-80 parts of kudzu roots, 40-70 parts of chrysanthemum notoginseng, and 10-30 parts of houttuynia cordata together can increase the average egg weight of laying hens' eggs, increase the yolk color, reduce the cholesterol content of the yolk, and increase the serine, glutamic acid, and alanine contents of the yolk. The content of total amino acids is greatly improved, and the obtained laying yolk is of better quality and has greater economic value.

[0009] Furthermore, the raw material ratio of the Chinese medicine extract is 120 parts of kudzu leaves, 55 parts of kudzu roots, 60 parts of chrysanthemum notoginseng, and 20 parts of houttuynia cordata.

[0010] The second object of the present invention is to provide a method for preparing the above-mentioned Chinese medicine extract, characterized in that it comprises the following steps:

[0011] S1. Pretreatment: Weigh the formulated amount of kudzu leaves, kudzu roots, chrysanthemum notoginseng, and houttuynia cordata, crush the kudzu roots and pass through a 40-60 mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0012] S2, enzymolysis: add 4-6 times purified water to the pretreated material for enzymolysis for 2-4 hours to obtain material 1; the enzymolysis uses cellulase and pectinase in a mass ratio of 2-3:1, and the total amount of the two enzymes added is 0.1%-0.3%;

[0013] S3, pressing: adding emulsifier and corn seed oil to material 1, stirring and mixing for 10-15 minutes, adding to a centrifugal press for pressing, and collecting the residue and extract 1 respectively;

[0014] The total amount of the emulsifier and corn seed oil added is 5%-10% of the mass of material 1;

[0015] S4, secondary enzymolysis: add 2-3 times purified water to the drug residue collected in S3, and perform enzymolysis for 1-2 hours to obtain material 2; the enzymolysis uses protease and esterase in a mass ratio of 1:3-4, and the total amount of the two enzymes added is 0.2%-0.4%;

[0016] S5, secondary pressing: add emulsifier and corn seed oil to material 2, stir and mix for 10-15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0017] The total amount of the emulsifier and corn seed oil added is 5%-15% of the mass of material 2;

[0018] S6. Combining the extracts obtained from the two squeezing processes, concentrating under reduced pressure, and spray drying to obtain a Chinese medicine extract.

[0019] The present invention adopts two enzymolysis and pressing processes when preparing Chinese medicine extracts. The whole extraction and preparation process does not require high temperature and high pressure treatment, effectively reduces production costs, and retains heat-sensitive components. Cellulase and pectinase are used in the first enzymolysis process. Cellulase can hydrolyze cellulose in plant cell walls, and pectinase can degrade pectin between cells and separate cells from tissues. The inventor found that the cellulase and pectinase are used in combination according to a mass ratio of 2-3:1, which can fully degrade plant cell walls, release cells from plant tissues and cell walls, and improve the extraction efficiency of total flavonoids, an effective ingredient; protease and esterase are used in the second enzymolysis process. Protease can degrade proteins in plant cell walls, and esterase can decompose ester substances in plant cell walls to make the cell walls loose. Protease and esterase are used in combination according to a mass ratio of 1:3-4, which can improve the extraction rate of total flavonoids, an effective ingredient. Protease has both protein degradation and impurity removal effects, and can improve the purity of the effective ingredient.

[0020] The inventors first use enzymatic hydrolysis to destroy the plant cell structure and fully release the water-soluble active ingredients in the cells, then add emulsifiers and corn seed oil, stir and mix them, so that the fat-soluble active ingredients in the cells are dissolved in the material, and remove the drug residue through pressing. The obtained extract is concentrated under reduced pressure and spray-dried to obtain a traditional Chinese medicine extract.

[0021] Furthermore, in S2, the pH value during the enzymatic hydrolysis process is 4.5-5.5, and the enzymatic hydrolysis temperature is 35-45°C.

[0022] During the enzymatic hydrolysis process, an acidity regulator is used to adjust the pH value, and the acidity regulator is at least one of citric acid, lactic acid, malic acid, and tartaric acid.

[0023] Furthermore, in S3 or S5, the emulsifier is selected from one or more of modified soybean lecithin, gum dawa, succinic acid monoglyceride, xylitol monostearate, and lactic acid fatty acid glyceride.

[0024] Furthermore, in S3 or S5, the mass ratio of the emulsifier to the corn seed oil is 1:2-4.

[0025] Preferably, the mass ratio of the emulsifier to the corn seed oil is 1:3.

[0026] Furthermore, in S4, the pH value during the enzymatic hydrolysis process is 6.0-8.0, and the enzymatic hydrolysis temperature is 45-55°C.

[0027] During the enzymatic hydrolysis process, a pH regulator is used to adjust the pH value, and the pH regulator is at least one of citric acid, sodium bicarbonate, malic acid, and tartaric acid.

[0028] Furthermore, in S6, the spray drying air inlet temperature is 160-180°C, and the air outlet temperature is 70-80°C.

[0029] The third object of the present invention is to provide the use of the above-mentioned Chinese medicine extract in laying hen feed.

[0030] Furthermore, the amount of the Chinese herbal medicine extract added to laying hen feed is 1000 mg / kg to 3000 mg / kg.

[0031] Preferably, the amount of the Chinese herbal medicine extract added to laying hen feed is 3000 mg / kg.

[0032] The inventors found that the serine, alanine and total amino acid contents in the egg yolk of the 3000 mg / kg Chinese herbal extract group increased significantly, and the egg yolk quality of the laying hen eggs obtained was better, with greater economic value and market prospects.

[0033] Beneficial effects of the present invention:

[0034] 1. The quality of egg yolk determines the quality of eggs. In the research of traditional laying hen feed, no research has been conducted on how to improve the quality of egg yolk, an important component of eggs. The inventors found that adding 80-150 parts of kudzu leaves, 30-80 parts of kudzu roots, 40-70 parts of chrysanthemum notoginseng, and 10-30 parts of houttuynia cordata to the prepared Chinese medicine extract at a concentration of 1000mg / kg to 3000mg / kg to laying hen feed can increase the average egg weight of laying hens, increase the yolk color, and reduce the cholesterol content of yolk. This shows that adding Chinese medicine extracts to laying hen feed can avoid hyperlipidemia, coronary heart disease, cerebral infarction, hypertension, osteoporosis, fatty liver and other diseases caused by excessive cholesterol content in egg yolk; the inventors also found that the serine, alanine and total amino acid contents of egg yolks in the 3000mg / kg Chinese medicine extract group were significantly increased, and the yolk quality of the obtained laying hen eggs was relatively good, with great economic value and market prospects.

[0035] 2. The present invention adopts two enzymatic hydrolysis and pressing processes when preparing Chinese medicine extracts. The entire extraction and preparation process does not require high temperature and high pressure treatment, which effectively reduces the production cost while retaining the heat-sensitive components.

[0036] 3. When preparing the Chinese medicine extract, the present invention uses cellulase and pectinase in the first enzymatic hydrolysis process. Cellulase can hydrolyze cellulose in plant cell walls, and pectinase can degrade pectin between cells and separate cells from tissues. The inventors found that the use of cellulase and pectinase in a mass ratio of 2-3:1 can fully degrade plant cell walls, release cells from plant tissues and cell walls, and improve the extraction efficiency of total flavonoids, an effective ingredient; protease and esterase are used in the second enzymatic hydrolysis process. Protease can degrade proteins in plant cell walls, and esterase can decompose ester substances in plant cell walls to make the cell walls loose. The use of protease and esterase in a mass ratio of 1:3-4 can improve the extraction rate of total flavonoids, an effective ingredient. Protease has both protein degradation and impurity removal effects, and can improve the purity of the effective ingredient.

[0037] 4. The inventors first use enzymatic hydrolysis to destroy the plant cell structure and fully release the water-soluble active ingredients in the cells, then add emulsifiers and corn seed oil, stir and mix them, so that the fat-soluble active ingredients in the cells are dissolved in the material, and remove the drug residue through pressing. The obtained extract is concentrated under reduced pressure and spray-dried to obtain a traditional Chinese medicine extract. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the specific implementation methods of the specification.

[0039] The experimental methods in the following examples are conventional methods unless otherwise specified. The reagents or instruments involved in the present invention without indicating the manufacturer are all common commercial products and can be purchased from the market.

[0040] Example 1: Preparation of a Chinese herbal extract for improving laying hen production performance

[0041] S1, pretreatment: weigh 1.2kg kudzu leaves, 550g kudzu roots, 600g chrysanthemum notoginseng, 200g houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0042] S2, enzymolysis: add 5 times of purified water to the pretreated material, adjust the pH to 5.0 with citric acid, and perform enzymolysis at 45° C. for 3 h to obtain material 1; the enzymolysis uses cellulase and pectinase, the addition amount of cellulase is 0.2%, and the addition amount of pectinase is 0.1%;

[0043] S3, pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 1, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 1 respectively;

[0044] S4, secondary enzymolysis: add 3 times purified water to the drug residue collected in S3, adjust the pH to 8.0 with sodium bicarbonate, and perform enzymolysis at 50°C for 2h to obtain material 2; the enzymolysis uses protease and esterase, the addition amount of protease is 0.1%, and the addition amount of esterase is 0.3%;

[0045] S5, secondary pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 2, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0046] S6. Combine the extracts obtained from the two squeezing treatments, concentrate under reduced pressure to a density of 1.12, and spray dry. The spray drying air inlet temperature is 170° C. and the air outlet temperature is 80° C. to obtain 705 g of Chinese medicine extract.

[0047] Example 2: Preparation of a Chinese herbal medicine extract for improving laying hen production performance

[0048] S1, pretreatment: weigh 800g of kudzu leaves, 700g of kudzu roots, 700g of chrysanthemum notoginseng, and 300g of houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0049] S2, enzymolysis: add 6 times of purified water to the pretreated material, adjust the pH to 5.5 with citric acid, and perform enzymolysis at 45°C for 3h to obtain material 1; the enzymolysis uses cellulase and pectinase, the addition amount of cellulase is 0.15%, and the addition amount of pectinase is 0.05%;

[0050] S3, pressing: adding 1.5% xylitol anhydride monostearate and 6% corn seed oil to material 1, stirring and mixing for 12 minutes, adding to a centrifugal press for pressing, and collecting the residue and extract 1 respectively;

[0051] S4, secondary enzymolysis: add 2 times purified water to the drug residue collected in S3, adjust the pH to 7.8 with sodium bicarbonate, and perform enzymolysis at 45°C for 1.5h to obtain material 2; the enzymolysis uses protease and esterase, the addition amount of protease is 0.05%, and the addition amount of esterase is 0.2%;

[0052] S5, secondary pressing: add 1.5% xylitol anhydride monostearate and 6% corn seed oil to material 2, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0053] S6. Combine the extracts obtained from the two squeezing processes, concentrate under reduced pressure to a density of 1.13, and spray dry. The spray drying air inlet temperature is 180° C. and the air outlet temperature is 75° C. to obtain 663 g of Chinese herbal medicine extract.

[0054] Example 3: Preparation of a Chinese herbal medicine extract for improving laying hen production performance

[0055] S1, pretreatment: weigh 1.3kg kudzu leaf, 400g kudzu root, 500g chrysanthemum notoginseng, 100g houttuynia cordata, crush the kudzu root and pass it through a 60-mesh sieve; crush the kudzu leaf, chrysanthemum notoginseng, and houttuynia cordata;

[0056] S2, enzymolysis: add 4 times purified water to the pretreated material, adjust the pH to 4.5 with citric acid, and perform enzymolysis at 40°C for 4 hours to obtain material 1; the enzymolysis uses cellulase and pectinase, the addition amount of cellulase is 0.2%, and the addition amount of pectinase is 0.1%;

[0057] S3, pressing: add 3% lactic acid fatty acid glyceride and 6% corn seed oil to material 1, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 1 respectively;

[0058] S4, secondary enzymolysis: add 2 times purified water to the drug residue collected in S3, adjust the pH to 7.5 with sodium bicarbonate, and perform enzymolysis at 55°C for 1.5h to obtain material 2; the enzymolysis uses protease and esterase, the addition amount of protease is 0.05%, and the addition amount of esterase is 0.2%;

[0059] S5, secondary pressing: add 2% xylitol anhydride monostearate and 6% corn seed oil to material 2, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0060] S6, combining the extracts obtained from the two squeezing processes, concentrating under reduced pressure to a density of 1.11, spray drying, the spray drying inlet air temperature is 180° C., and the outlet air temperature is 75° C., to obtain a Chinese medicine extract;

[0061] S6. The extracts obtained from the two squeezing processes were combined, concentrated under reduced pressure, and spray-dried to obtain 641 g of Chinese herbal medicine extract.

[0062] Comparative Example 1:

[0063] S1, pretreatment: weigh 1.2kg kudzu leaves, 550g kudzu roots, 600g chrysanthemum notoginseng, 200g houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0064] S2, enzymolysis: add 5 times of purified water to the pretreated material, adjust the pH to 5.0 with citric acid, and perform enzymolysis at 45° C. for 3 h to obtain material 1; the enzymolysis uses cellulase and pectinase, the addition amount of cellulase is 0.2%, and the addition amount of pectinase is 0.1%;

[0065] S3, pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 1, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract respectively;

[0066] S4. The extract obtained by squeezing was concentrated under reduced pressure to a density of 1.12, and spray-dried. The spray-drying air inlet temperature was 170° C. and the air outlet temperature was 80° C. to obtain 532 g of Chinese herbal medicine extract.

[0067] Comparative Example 2:

[0068] S1, pretreatment: weigh 1.2kg kudzu leaves, 550g kudzu roots, 600g chrysanthemum notoginseng, 200g houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0069] S2, enzymolysis: add 5 times of purified water to the pretreated material, adjust the pH to 5.0 with citric acid, and perform enzymolysis at 45° C. for 3 h to obtain material 1; the enzymolysis uses cellulase and pectinase, the addition amount of cellulase is 0.2%, and the addition amount of pectinase is 0.1%;

[0070] S3, pressing: adding material 1 into a centrifugal press for pressing, and collecting the residue and extract 1 respectively;

[0071] S4, secondary enzymolysis: add 3 times purified water to the drug residue collected in S3, adjust the pH to 8.0 with sodium bicarbonate, and perform enzymolysis at 50°C for 2h to obtain material 2; the enzymolysis uses protease and esterase, the addition amount of protease is 0.1%, and the addition amount of esterase is 0.3%;

[0072] S5, secondary pressing: adding material 2 into a centrifugal press for pressing, and collecting the drug residue and extract 2 respectively;

[0073] S6. Combine the extracts obtained from the two squeezing treatments, concentrate under reduced pressure to a density of 1.12, and spray dry. The spray drying air inlet temperature is 170° C. and the air outlet temperature is 80° C. to obtain 601 g of Chinese medicine extract.

[0074] Comparative Example 3:

[0075] S1, pretreatment: weigh 1.2kg kudzu leaves, 550g kudzu roots, 600g chrysanthemum notoginseng, 200g houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0076] S2, enzymatic hydrolysis: add 5 times of purified water to the pretreated material, adjust the pH to 5.0 with citric acid, and perform enzymatic hydrolysis at 45°C for 3h to obtain material 1; the enzymatic hydrolysis uses cellulase at an addition amount of 0.2%;

[0077] S3, pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 1, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 1 respectively;

[0078] S4, secondary enzymolysis: add 3 times purified water to the drug residue collected in S3, adjust the pH to 8.0 with sodium bicarbonate, and perform enzymolysis at 50°C for 2h to obtain material 2; the enzymolysis uses protease with an addition amount of 0.1%;

[0079] S5, secondary pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 2, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0080] S6. Combine the extracts obtained from the two squeezing treatments, concentrate under reduced pressure to a density of 1.12, and spray dry. The spray drying inlet air temperature is 170° C. and the outlet air temperature is 80° C. to obtain 643 g of Chinese herbal medicine extract.

[0081] Comparative Example 4:

[0082] S1, pretreatment: weigh 1.2kg kudzu leaves, 550g kudzu roots, 600g chrysanthemum notoginseng, 200g houttuynia cordata, crush the kudzu roots and pass through a 40-mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata;

[0083] S2, enzymolysis: add 5 times of purified water to the pretreated material, adjust the pH to 5.0 with citric acid, and perform enzymolysis at 45°C for 3h to obtain material 1; the enzymolysis uses pectinase with an addition amount of 0.1%;

[0084] S3, pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 1, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 1 respectively;

[0085] S4, secondary enzymolysis: add 3 times purified water to the drug residue collected in S3, adjust the pH to 8.0 with sodium bicarbonate, and perform enzymolysis at 50°C for 2h to obtain material 2; the enzymolysis uses esterase with an addition amount of 0.3%;

[0086] S5, secondary pressing: add 2% lactic acid fatty acid glyceride and 6% corn seed oil to material 2, stir and mix for 15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively;

[0087] S6. Combine the extracts obtained from the two squeezing treatments, concentrate under reduced pressure to a density of 1.12, and spray dry. The spray drying air inlet temperature is 170° C. and the air outlet temperature is 80° C. to obtain 638 g of Chinese herbal medicine extract.

[0088] Test Example 1:

[0089] The total flavonoid extraction rate and the yield of the Chinese medicine extracts prepared in Examples 1-3 and Comparative Examples 1-4 were investigated. The total flavonoid test method refers to the "Determination of Total Flavonoids in Health Foods" in the "Technical Guidelines for Inspection and Evaluation of Physical, Chemical and Hygiene Indicators of Health Foods (2020 Edition)", and the results are shown in Table 1.

[0090] Table 1

[0091]

[0092]

[0093] As can be seen from Table 1, the total flavonoid extraction rate of the Chinese herbal extracts obtained in Examples 1-3 is 5.05%-5.36%, and the yield of the Chinese herbal extract is 26.52%-27.87%, which are higher than those in Comparative Examples 1-4.

[0094] Compared with Example 1, the total flavonoid extraction rate of the Chinese medicine extract prepared in Comparative Example 1 was 3.84%, and the Chinese medicine extract yield was 20.86%, which were lower than those in Example 1, indicating that the extraction efficiency was higher by adopting the two-step enzymatic hydrolysis and pressing technology.

[0095] Compared with Example 1, the total flavonoids extraction rate of the Chinese medicine extract prepared in Comparative Example 2 was 4.39%, and the Chinese medicine extract yield was 23.57%, which were lower than those in Example 1. This indicates that the addition of emulsifiers and corn seed oil during pressing is beneficial to the dissolution of total flavonoids, thereby improving the extraction rate.

[0096] Compared with Example 1, the total flavonoid extraction rate of the Chinese medicine extract prepared in Comparative Example 3 was 4.67%, and the Chinese medicine extract yield was 25.21%; the total flavonoid extraction rate of the Chinese medicine extract prepared in Comparative Example 4 was 4.59%, and the Chinese medicine extract yield was 25.02%, both lower than Example 1, indicating that the use of mixed enzymes during enzymatic hydrolysis is beneficial to improving the extraction rate of the Chinese medicine extract.

[0097] Experimental Example 2: Effects of Chinese herbal extracts on laying hen production performance and egg quality

[0098] 900 laying hens with similar egg-laying performance at different weeks of age were randomly divided into 5 treatments, each with 6 replicates and 30 hens in each replicate; in the 5 treatments, the control group was fed a basal diet, and the experimental groups were fed with 1000 mg / kg, 2000 mg / kg, 3000 mg / kg of the Chinese herbal extract prepared in Example 1 and 1000 mg / kg of the Chinese herbal extract prepared in Example 1 + 40 mg / kg lutein in the basal diet, respectively, with a pre-test period of 1 week and a full test period of 8 weeks.

[0099] The experimental chickens were kept in closed chicken houses with three-layer three-dimensional cages, with constant lighting for 16 hours and an automatic lighting control system. They were fed artificially and had free access to food and water. The chicken houses were disinfected according to the routine epidemic prevention procedures to ensure the hygiene of the livestock houses. The health status and feeding status of the chickens were observed every day during the experiment.

[0100] 1. Egg quality determination

[0101] At the end of the test period, 4 eggs were randomly collected from each replicate for routine egg quality analysis. The eggs were picked manually. The number of eggs, egg weight, number and weight of unqualified eggs were recorded in replicates every day during the test period. The egg production rate, daily egg production, average egg weight, and qualified egg rate were calculated. The feed intake during the test period was counted, and the average daily feed intake and feed-egg ratio were calculated. The results are shown in Table 2.

[0102] Table 2

[0103]

[0104] As can be seen from Table 2, compared with the control group, adding different amounts of Chinese herbal extracts to the laying hen diet significantly increased the average egg weight of laying hens (P<0.05), indicating that Chinese herbal extracts can increase the average egg weight, but have no significant effect on daily egg weight, qualified egg rate, daily egg production, egg production rate, average daily feed intake and feed-egg ratio (P>0.05).

[0105] 2. Effects of Chinese herbal medicine extracts on egg quality

[0106] At the end of the experimental period, four eggs were randomly collected from each replicate for routine egg quality analysis to measure egg shape index, eggshell strength, eggshell thickness, yolk color and ratio, albumen height, and Haugh unit. The results are shown in Table 3.

[0107] Table 3

[0108]

[0109] It can be seen from Table 3 that, compared with the control group, adding different amounts of Chinese herbal extracts to laying hen diets had no significant effect on egg shape index, eggshell strength, Haugh unit, yolk ratio and other indicators (P>0.05), but could increase the yolk color (P<0.1); at the same time, it can be seen that adding 2000mg / kg, 3000mg / kg or 1000mg / kg of Chinese herbal extracts + 40mg / kg of biotin significantly reduced the eggshell thickness (P<0.05), while adding 1000mg / kg of Chinese herbal extracts had no significant effect on the eggshell thickness (P>0.05), indicating that as the dosage of Chinese herbal extracts increases, the eggshell thickness will decrease.

[0110] 3. Effects of Chinese herbal extracts on nutrient content of egg yolk

[0111] At the end of the test period, 4 eggs were randomly collected from each repetition, and the yolk nutrients of the eggs produced by the test groups fed with the basal feed and the basal feed respectively added with 1000 mg / kg, 2000 mg / kg, and 3000 mg / kg of the Chinese medicine extract prepared in Example 1 were analyzed. The results are shown in Tables 4 and 5.

[0112] Table 4 Analysis of nutrient content of egg yolk

[0113]

[0114]

[0115] As can be seen from Table 4, compared with the control group, adding different amounts of Chinese herbal extracts to laying hen feed had no significant effect on the moisture, crude protein, crude fat and trimethylamine content of egg yolk (P>0.05); at the same time, it can be seen that the cholesterol content in egg yolk of the control group fed with the basic feed was 13.48 mg / g, while the cholesterol content in egg yolk of the experimental group was 12.42-12.83 mg / g, and the cholesterol content was significantly decreased (P<0.05), indicating that adding Chinese herbal extracts to laying hen feed can reduce the cholesterol content in egg yolk, and can avoid hyperlipidemia, coronary heart disease, cerebral infarction, hypertension, osteoporosis, fatty liver and other diseases caused by excessive cholesterol content in egg yolk.

[0116] Table 5 Analysis of amino acid content in egg yolk

[0117]

[0118]

[0119] As can be seen from Table 5, the serine content in the egg yolk of the control group was 11.06 mg / g, while the serine content in the egg yolk of the 2000 mg / kg and 3000 mg / kg Chinese herbal extract groups was 11.69 and 11.89 mg / g, respectively, and the serine content increased significantly (P<0.05). Serine can supplement human nutrition, improve human immunity, and promote fat metabolism and stimulate muscle growth.

[0120] The alanine content in the egg yolk of the control group was 7.35 mg / g, and the alanine content in the 3000 mg / kg Chinese herbal extract group was 7.80 mg / g, and the alanine content increased significantly (P<0.05); the alanine content in the 1000 mg / kg and 2000 mg / kg Chinese herbal extract groups was no different from that in the control group. Alanine can stimulate muscle protein synthesis and promote muscle growth; participate in immune cell metabolism, improve its activity and damage resistance, maintain the normal function of the immune system; stimulate collagen synthesis and enhance wound healing ability.

[0121] The total amino acid content in the egg yolk of the control group was 133.98 mg / g, and the total amino acid contents in the egg yolks of the 1000 mg / kg and 2000 mg / kg Chinese herbal extract groups were 132.68 mg / g and 134.75 mg / g, respectively. There was no significant difference in the total amino acid content compared with the control group; the total amino acid content in the egg yolk of the 3000 mg / kg Chinese herbal extract group was 135.90 mg / g, which was significantly increased compared with the control group (P<0.05).

[0122] It can be seen from Tables 4 and 5 that adding Chinese herbal extracts to laying hen feed can significantly reduce the cholesterol content in egg yolks. At the same time, it can be seen that the serine, alanine and total amino acid contents in the egg yolks of the 3000 mg / kg Chinese herbal extract group will increase significantly.

[0123] The preferred embodiments of the present invention are described in detail above, but the present invention is not limited to the above embodiments. Other changes or modifications can be made within the knowledge of ordinary technicians in this field. It is not necessary and impossible to list all the embodiments here. Any modification, equivalent substitution and improvement made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A Chinese herbal medicine extract for improving the production performance of laying hens, characterized in that: The raw material ratio of the Chinese medicine extract is 80-150 parts of kudzu leaves, 30-80 parts of kudzu roots, 40-70 parts of chrysanthemum notoginseng, and 10-30 parts of houttuynia cordata.

2. A Chinese medicinal extract for improving the production performance of laying hens according to claim 1, characterized in that: The raw material ratio of the Chinese medicine extract is 120 parts of kudzu leaves, 55 parts of kudzu roots, 60 parts of chrysanthemum notoginseng, and 20 parts of houttuynia cordata.

3. The method for preparing the Chinese medicine extract according to claim 1 or 2, characterized in that: The steps include: S1. Pretreatment: Weigh the formulated amount of kudzu leaves, kudzu roots, chrysanthemum notoginseng, and houttuynia cordata, crush the kudzu roots and pass through a 40-60 mesh sieve; crush the kudzu leaves, chrysanthemum notoginseng, and houttuynia cordata; S2, enzymolysis: add 4-6 times purified water to the pretreated material for enzymolysis for 2-4 hours to obtain material 1; the enzymolysis uses cellulase and pectinase in a mass ratio of 2-3:1, and the total amount of the two enzymes added is 0.1%-0.3%; S3, pressing: adding emulsifier and corn seed oil to material 1, stirring and mixing for 10-15 minutes, adding to a centrifugal press for pressing, and collecting the residue and extract 1 respectively; The total amount of the emulsifier and corn seed oil added is 5%-10% of the mass of material 1; S4, secondary enzymolysis: add 2-3 times purified water to the drug residue collected in S3, and perform enzymolysis for 1-2 hours to obtain material 2; the enzymolysis uses protease and esterase in a mass ratio of 1:3-4, and the total amount of the two enzymes added is 0.2%-0.4%; S5, secondary pressing: add emulsifier and corn seed oil to material 2, stir and mix for 10-15 minutes, add to a centrifugal press for pressing, and collect the residue and extract 2 respectively; The total amount of the emulsifier and corn seed oil added is 5%-15% of the mass of material 2; S6. Combining the extracts obtained from the two squeezing processes, concentrating under reduced pressure, and spray drying to obtain a Chinese medicine extract.

4. The preparation method according to claim 3, characterized in that: In S2, the pH value during the enzymatic hydrolysis process is 4.5-5.5, and the enzymatic hydrolysis temperature is 35-45°C.

5. The preparation method according to claim 3, characterized in that: In S3 or S5, the emulsifier is selected from one or more of modified soybean lecithin, gum dawa, succinic acid monoglyceride, xylitol monostearate, and lactic acid fatty acid glyceride.

6. The preparation method according to claim 3, characterized in that: In S3 or S5, the mass ratio of the emulsifier to the corn seed oil is 1:2-4.

7. The preparation method according to claim 3, characterized in that: In S4, the pH value during the enzymatic hydrolysis process is 6.0-8.0, and the enzymatic hydrolysis temperature is 45-55°C.

8. The preparation method according to claim 3, characterized in that: In S6, the spray drying air inlet temperature is 160-180°C, and the air outlet temperature is 70-80°C.

9. Use of the Chinese medicinal extract according to claim 1 or 2 in laying hen feed.

10. The use according to claim 9, characterized in that: The amount of Chinese herbal medicine extract added to laying hen feed is 1000mg / kg~3000mg / kg.

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