A kind of repair water containing recombinant type III humanized collagen and its preparation method

Abstract

The present application relates to the technical field of skin repair, and specifically discloses a repair water containing recombinant humanized type III collagen and a preparation method thereof. The repair water is composed of the following raw materials in percentage by mass: 0.08-0.12% recombinant humanized type III collagen, 0.13-0.18% hydroxyethyl cellulose, 1.9-2.1% propylene glycol, 0.08-0.12% pentanediol, 0.3-0.4% PE9010, 0.08-0.12% promoting composition, 0.1-0.12% antioxidant, 0.08-0.12% cyclodextrin, and the remainder is purified water. The use of the promoting composition in the present application can promote the repair of the skin, and cyclodextrin can increase the stability of the repair water and prevent the degradation of the active ingredients, thereby improving the repair effect on the skin. At the same time, it can also reduce the irritation of recombinant humanized type III collagen to the skin and improve the comfort of the product.

Description

Technical Field

[0001] The present application relates to the technical field of skin repair, and in particular to a repair water containing recombinant type III humanized collagen and a preparation method thereof. Background Art

[0002] Collagen is the main component of the extracellular matrix, which plays a supporting and stabilizing role. The quantity and quality of the extracellular matrix determine the barrier function of the skin. Like natural collagen, recombinant collagen has a unique triple helix basic structure and a higher-level structure that is cross-linked into a network, which is the collagen fiber. When the stratum corneum cells of the skin are damaged, the collagen fibers are no longer strong, and they break, become stringy, and lose their supporting and stabilizing properties, which can cause skin diseases such as reduced skin elasticity, aging, dryness, impaired barrier function, pigmentation, inflammation, and aging. The preparation of skin repair dressings with recombinant humanized type III collagen as the core ingredient has certain advantages, but its repair speed is not ideal and it is irritating to the skin. Summary of the invention

[0003] In order to improve the repair effect on the skin, the present application provides a repair water containing recombinant type III humanized collagen and a preparation method thereof.

[0004] In the first aspect, the present application provides a repair water containing recombinant humanized type III collagen, which adopts the following technical solution: A repair water containing recombinant humanized type III collagen, the repair water is composed of the following raw materials in percentage by mass: 0.08-0.12% of recombinant humanized type III collagen, 0.13-0.18% of hydroxyethyl cellulose, 1.9-2.1% of propylene glycol, 0.08-0.12% of pentanediol, 0.3-0.4% of PE9010, 0.08-0.12% of a promoting composition, 0.1-0.12% of an antioxidant, 0.08-0.12% of cyclodextrin, and the remainder being purified water.

[0005] By adopting the above technical scheme and utilizing the promoting composition, the repair of the skin can be promoted. Cyclodextrin can increase the stability of the repair water and prevent the degradation of the active ingredients, thereby improving the repair effect on the skin. At the same time, it can also reduce the irritation of the recombinant type III humanized collagen to the skin and improve the comfort of the product.

[0006] In a specific embodiment, the promoting composition includes a mixture of allantoin, growth factors, hydroxy acids, and repair extracts.

[0007] By adopting the above technical scheme, allantoin helps to promote epidermal cell regeneration and accelerate the wound healing process. Growth factors can stimulate the proliferation and differentiation of skin cells, accelerate wound healing, and reduce scar formation. Hydroxy acids have an exfoliating effect, promoting the shedding of dead skin cells, making the new skin smoother and more delicate. Repair extracts have a good repair effect. Therefore, the promotion composition composed of allantoin, growth factors, hydroxy acids, and repair extracts can better repair the skin.

[0008] In a specific embodiment, the growth factor comprises one of epidermal growth factor and fibroblast growth factor.

[0009] In a specific embodiment, the hydroxy acid includes one or more of lactic acid, salicylic acid, and malic acid.

[0010] In a specific embodiment, the preparation of the repair extract comprises the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in an ethanol aqueous solution with a concentration of 50%-70% for extraction, filtered, heated and refluxed to obtain an extract; the extract is subjected to reduced pressure distillation to obtain a repair extract.

[0011] By adopting the above technical scheme, the repair extract prepared by using Panax notoginseng, Salvia miltiorrhiza, Paeonia lactiflora, Astragalus membranaceus and Cinnamomum cassia has a good repair effect on the skin.

[0012] In a specific embodiment, the weight ratio of the crushed material to the ethanol aqueous solution is 1:(10-15).

[0013] By adopting the above technical solution, the ratio of the crushed material to the ethanol aqueous solution is further limited, thereby improving the extraction effect of the crushed material.

[0014] In a specific embodiment, the antioxidant comprises a mixture of magnesium ascorbyl phosphate and tocopherol.

[0015] By adopting the above technical scheme, magnesium ascorbyl phosphate and tocopherol make the repair water have better antioxidant effect, and magnesium ascorbyl phosphate can also effectively promote skin cell regeneration, accelerate wound healing process, while reducing skin inflammatory response and improving the overall health of the skin. Tocopherol can also reduce free radical damage and protect the skin from secondary damage. Therefore, the combination of the two can further improve the repair effect of repair water on the skin.

[0016] In the second aspect, the present application provides a method for preparing a repair water containing recombinant type III humanized collagen, which adopts the following technical scheme: A method for preparing repair water containing recombinant type III humanized collagen comprises the following steps: Weigh 5-6% of the total mass of the preparation water with purified water to dissolve the recombinant type III humanized collagen to form a uniform transparent solution to obtain solution A; use propylene glycol and pentylene glycol to pre-disperse hydroxyethyl cellulose to form a uniform suspension to obtain solution B; Weigh 78-80% of the total mass of the prepared water, add purified water to the preparation tank, stir and heat to 80-90°C, add solution B, reflux and cool, when the temperature drops to 55-65°C, add solution A, then add the remaining purified water of the total mass of the prepared water, continue stirring, and obtain a colorless transparent solution; When the temperature drops to 40-55°C, PE9010, the promoting composition, the antioxidant and the cyclodextrin are added, and the mixture is stirred while keeping the temperature to obtain the repair water.

[0017] By adopting the above technical scheme, the recombinant humanized type III collagen is first dissolved to obtain solution A, and then hydroxyethyl cellulose is pre-dispersed with propylene glycol and pentylene glycol to obtain solution B, and then solution A and solution B are mixed with water to obtain a colorless and transparent solution, and finally PE9010, a promoting composition, an antioxidant, and cyclodextrin are added, and the solution is stirred while keeping warm to obtain a repair water with a good skin repair effect.

[0018] In summary, the present application includes at least one of the following beneficial technical effects: 1. The promotion composition used in this application can promote the repair of the skin. Cyclodextrin can increase the stability of the repair water and prevent the degradation of the active ingredients, thereby improving the repair effect on the skin. At the same time, it can also reduce the irritation of recombinant type III humanized collagen to the skin and improve the comfort of the product; 2. In the present application, allantoin helps promote epidermal cell regeneration and accelerate wound healing. Growth factors can stimulate the proliferation and differentiation of skin cells, accelerate wound healing, and reduce scar formation. Hydroxy acids have an exfoliating effect, promote the shedding of dead skin cells, and make the new skin smoother and more delicate. Repair extracts have a good repair effect. Therefore, the promotion composition composed of allantoin, growth factors, hydroxy acids, and repair extracts can better repair the skin. 3. The method in the present application first dissolves the recombinant humanized type III collagen to obtain solution A, then pre-disperses hydroxyethyl cellulose with propylene glycol and pentylene glycol to obtain solution B, and then mixes solution A and solution B with water to obtain a colorless and transparent solution, and finally adds PE9010, a promoting composition, an antioxidant, and cyclodextrin, and stirs while keeping warm to obtain a repair water with a good skin repair effect. DETAILED DESCRIPTION

[0019] The present application is further described in detail below with reference to the embodiments.

[0020] All raw materials in the examples can be obtained commercially. Growth factors include but are not limited to one of epidermal growth factor and fibroblast growth factor, and epidermal growth factor is preferred in the present application; hydroxy acids include but are not limited to a mixture of one or more of lactic acid, salicylic acid, and malic acid, and lactic acid is preferred in the present application.

[0021] Preparation Example Preparation Example 1 Preparation Example 1 provides a method for preparing a repair extract, comprising the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in a 60% ethanol aqueous solution for extraction, filtered, and the filtrate is added to a hot reflux device, and the filtrate is extracted by hot reflux method at 75°C for 2 hours to obtain a reflux liquid, and the reflux liquid is filtered to obtain a filtrate, and the extraction is continued three times, and the three filtrates are combined to obtain an extract; wherein the weight ratio of the crushed material to the ethanol aqueous solution is 1:7.5; The extract was concentrated at -0.125 MPa and 52.5°C to obtain a repair extract.

[0022] Preparation Example 2 Preparation Example 2 provides a method for preparing a repair extract, comprising the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in a 60% ethanol aqueous solution for extraction, filtered, and the filtrate is added to a hot reflux device, and the filtrate is extracted by hot reflux method at 75°C for 2 hours to obtain a reflux liquid, and the reflux liquid is filtered to obtain a filtrate, and the extraction is continued three times, and the three filtrates are combined to obtain an extract; wherein the weight ratio of the crushed material to the ethanol aqueous solution is 1:10; The extract was concentrated at -0.125 MPa and 52.5°C to obtain a repair extract.

[0023] Preparation Example 3 Preparation Example 3 provides a method for preparing a repair extract, comprising the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in a 60% ethanol aqueous solution for extraction, filtered, and the filtrate is added to a hot reflux device, and the filtrate is extracted by hot reflux method at 75°C for 2 hours to obtain a reflux liquid, and the reflux liquid is filtered to obtain a filtrate, and the extraction is continued three times, and the three filtrates are combined to obtain an extract; wherein the weight ratio of the crushed material to the ethanol aqueous solution is 1:12.5; The extract was concentrated at -0.125 MPa and 52.5°C to obtain a repair extract.

[0024] Preparation Example 4 Preparation Example 4 provides a method for preparing a repair extract, comprising the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in a 60% ethanol aqueous solution for extraction, filtered, and the filtrate is added to a hot reflux device, and the filtrate is extracted by hot reflux method at 75°C for 2 hours to obtain a reflux liquid, and the reflux liquid is filtered to obtain a filtrate, and the extraction is continued three times, and the three filtrates are combined to obtain an extract; wherein the weight ratio of the crushed material to the ethanol aqueous solution is 1:15; The extract was concentrated at -0.125 MPa and 52.5°C to obtain a repair extract.

[0025] Preparation Example 5 Preparation Example 5 provides a method for preparing a repair extract, comprising the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in a 60% ethanol aqueous solution for extraction, filtered, and the filtrate is added to a hot reflux device, and the filtrate is extracted by hot reflux method at 75°C for 2 hours to obtain a reflux liquid, and the reflux liquid is filtered to obtain a filtrate, and the extraction is continued for three times, and the three filtrates are combined to obtain an extract; wherein the weight ratio of the crushed material to the ethanol aqueous solution is 1:17.5; The extract was concentrated at -0.125 MPa and 52.5°C to obtain a repair extract. Example

[0026] Example 1 Example 1 provides a method for preparing repair water containing recombinant type III humanized collagen, comprising the following steps: Weigh 48.7 g of purified water and dissolve 0.8 g of recombinant type III humanized collagen to form a uniform transparent solution to obtain solution A; use 19 g of propylene glycol and 0.8 g of pentylene glycol to pre-disperse 1.3 hydroxyethyl cellulose to form a uniform suspension to obtain solution B; Weigh 759.2g of purified water, add it to the liquid preparation tank, heat it to 80℃ with stirring, stir for 20min, add solution B, reflux and cool down, when the temperature drops to 55℃, add solution A, then add 165.4g of purified water, continue stirring for 10min to obtain a colorless transparent solution; When the temperature drops to 40°C, add 3g PE9010, 0.8g promotion composition, 1g antioxidant, and 0.8g cyclodextrin, and stir for 10 minutes to obtain repair water; wherein the promotion composition is a mixture of allantoin, growth factor, hydroxy acid, and the repair extract in Preparation Example 1, and the weight ratio of allantoin, growth factor, hydroxy acid, and repair extract is 1:2:2:3, the growth factor is epidermal growth factor, and the hydroxy acid is lactic acid; the antioxidant is a mixture of magnesium ascorbyl phosphate and tocopherol, and the weight ratio of magnesium ascorbyl phosphate and tocopherol is 1:1.

[0027] Example 2 The difference between Example 2 and Example 1 is that the repair extract is the repair extract in Preparation Example 2, and the remaining steps are consistent with Example 1.

[0028] Example 3 The difference between Example 3 and Example 1 is that the repair extract is the repair extract in Preparation Example 3, and the remaining steps are consistent with Example 1.

[0029] Example 4 The difference between Example 4 and Example 1 is that the repair extract is the repair extract in Preparation Example 4, and the remaining steps are consistent with Example 1.

[0030] Example 5 The difference between Example 5 and Example 1 is that the repair extract is the repair extract in Preparation Example 5, and the remaining steps are consistent with Example 1.

[0031] Example 6 Example 6 provides a method for preparing repair water containing recombinant type III humanized collagen, comprising the following steps: Weigh 48.5 g of purified water and dissolve 1 g of recombinant type III humanized collagen to form a uniform transparent solution to obtain solution A; use 20 g of propylene glycol and 1 g of pentylene glycol to pre-disperse 1.5 hydroxyethyl cellulose to form a uniform suspension to obtain solution B; Weigh 756.5g of purified water, add it to the liquid preparation tank, heat it to 80℃ with stirring, stir for 20min, add solution B, reflux and cool down, when the temperature drops to 55℃, add solution A, then add 164.9g of purified water, continue stirring for 10min to obtain a colorless transparent solution; When the temperature drops to 40°C, add 3.5g PE9010, 1g promotion composition, 1.1g antioxidant, and 1g cyclodextrin, and stir at this temperature for 10 minutes to obtain repair water; wherein the promotion composition is a mixture of allantoin, growth factor, hydroxy acid, and the repair extract in Preparation Example 3, and the weight ratio of allantoin, growth factor, hydroxy acid, and repair extract is 1:2:2:3, the growth factor is epidermal growth factor, and the hydroxy acid is lactic acid; the antioxidant is a mixture of magnesium ascorbyl phosphate and tocopherol, and the weight ratio of magnesium ascorbyl phosphate and tocopherol is 1:1.

[0032] Example 7 Example 7 provides a method for preparing repair water containing recombinant type III humanized collagen, comprising the following steps: Weigh 48.4 g of purified water and dissolve 1.2 g of recombinant type III humanized collagen to form a uniform transparent solution to obtain solution A; use 21 g of propylene glycol and 1.2 g of pentylene glycol to pre-disperse 1.8 g of hydroxyethyl cellulose to form a uniform suspension to obtain solution B; Weigh 754.5 g of purified water, add it to the preparation tank, heat it to 80°C with stirring, stir for 20 min, add solution B, reflux and cool down, when the temperature drops to 55°C, add solution A, then add 164.3 g of purified water, continue stirring for 10 min, and obtain a colorless transparent solution; When the temperature drops to 40°C, add 4g PE9010, 1.2g promotion composition, 1.2g antioxidant, and 1.2g cyclodextrin, and stir for 10 minutes to obtain repair water; wherein the promotion composition is a mixture of allantoin, growth factor, hydroxy acid, and the repair extract in Preparation Example 3, and the weight ratio of allantoin, growth factor, hydroxy acid, and repair extract is 1:2:2:3, the growth factor is epidermal growth factor, and the hydroxy acid is lactic acid; the antioxidant is a mixture of magnesium ascorbyl phosphate and tocopherol, and the weight ratio of magnesium ascorbyl phosphate and tocopherol is 1:1.

[0033] Example 8 The difference between Example 8 and Example 6 is that the promoting composition is a mixture of allantoin and growth factors, and the weight ratio of allantoin to growth factors is 1:2. The remaining steps are consistent with Example 6.

[0034] Example 9 The difference between Example 9 and Example 6 is that the promoting composition is a mixture of hydroxy acid and the repair extract in Preparation Example 3, and the weight ratio of hydroxy acid to the repair extract is 2:3. The remaining steps are consistent with Example 6.

[0035] Example 10 The difference between Example 10 and Example 6 is that the antioxidant is magnesium ascorbyl phosphate, and the remaining steps are consistent with Example 6.

[0036] Embodiment 11 The difference between Example 11 and Example 6 is that the antioxidant is tocopherol, and the remaining steps are consistent with Example 6.

[0037] Example 12 The difference between Example 12 and Example 6 is that 756.5 g of purified water is weighed and added to a liquid preparation tank, heated to 85° C. with stirring, stirred for 20 min, solution B is added, refluxed and cooled, and when the temperature drops to 60° C., solution A is added, followed by 164.9 g of purified water, and stirring is continued for 10 min to obtain a colorless transparent solution; When the temperature dropped to 47.5° C., 3.5 g PE9010, 1 g of the promoting composition, 1.1 g of the antioxidant, and 1 g of cyclodextrin were added, and the mixture was stirred for 10 min to obtain repair water. The remaining steps were consistent with those in Example 6.

[0038] Embodiment 13 The difference between Example 13 and Example 6 is that 756.5 g of purified water is weighed and added to a liquid preparation tank, heated to 90° C. with stirring, stirred for 20 min, solution B is added, refluxed and cooled, and when the temperature drops to 65° C., solution A is added, followed by 164.9 g of purified water, and stirring is continued for 10 min to obtain a colorless transparent solution; When the temperature drops to 55° C., 3.5 g PE9010, 1 g of the promoting composition, 1.1 g of the antioxidant, and 1 g of cyclodextrin are added, and the mixture is stirred for 10 min at the temperature to obtain repair water; the remaining steps are consistent with those in Example 6.

[0039] Comparative Example Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that when the temperature is lowered to 40° C., 3 g of PE9010 is added, and the mixture is stirred for 10 minutes under the same temperature to obtain repair water; the remaining steps are consistent with Example 1.

[0040] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that when the temperature is lowered to 40° C., 3 g PE9010, 0.8 g promoting composition and 1 g antioxidant are added, and the mixture is stirred for 10 min under the same temperature to obtain repair water; the remaining steps are consistent with Example 1.

[0041] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that when the temperature is lowered to 40°C, 3g PE9010, 1g antioxidant, and 0.8g cyclodextrin are added, and the mixture is stirred for 10 minutes under the same temperature to obtain repair water; the remaining steps are consistent with Example 1.

[0042] Performance test repair effect: The back of female mice was shaved, and the shaved area on both sides of the spine was designed symmetrically with the long axis parallel to the spine, about 1.5 cm × 1.5 cm; CO 2 Fractional laser irradiation, CO 2 Fractional laser energy 10mJ / cm 2 , rectangular spot 10mm×20mm, scanning spacing 0.3mm, coverage 25%, scanning mode: sequential scanning, uniform irradiation twice without repetition. On the first day after fractional laser irradiation, the surface of the mouse skin became red; on the second day, the skin became red and slightly edematous; on the third day, the inflammatory reaction was most obvious, manifested as erythema, erosion and exudation. The mice were randomly divided into a blank control group and an experimental group. The experimental groups corresponded to Examples 1-13 and Comparative Examples 1-3, respectively, with five mice in each group. The blank control group was directly bandaged with dry gauze; the experimental groups used the repair water in the corresponding embodiments or comparative examples, respectively, once a day, and recorded the skin morphology and measured the wound size after each medication. The wound area 7 days after surgery was calculated, and the wound healing rate was calculated, recorded as a; the wound healing rate of the blank control group was recorded as b, and b was 0.49±0.11cm 2 / d; calculate the percentage c of wound healing speed improvement, c = (ab) / b * 100%, the higher the percentage c of wound healing speed improvement, the better the skin repair performance of the repair water.

[0043] Table 1 Performance test results of repair water sample C(％) Example 1 96.16 Example 2 97.13 Example 3 97.77 Example 4 97.75 Example 5 96.55 Example 6 98.99 Example 7 98.29 Example 8 97.57 Example 9 97.79 Example 10 98.01 Embodiment 11 97.91 Example 12 99.78 Embodiment 13 99.15 Comparative Example 1 91.26 Comparative Example 2 92.79 Comparative Example 3 91.87 Combining Example 1 and Comparative Examples 1-3, the repair effect of the repair water in Example 1 is the best. It can be seen that when preparing the repair water, adding the promoting composition and cyclodextrin to the beverage, the promoting composition can promote the repair of the skin, and the cyclodextrin can increase the stability of the repair water and prevent the degradation of the active ingredients, thereby improving the repair effect on the skin.

[0044] Combined with Examples 1-5, the repair effect of the repair water in Examples 2-4 is better. It can be seen that when preparing the repair extract, the ratio of the crushed material to the ethanol aqueous solution is preferably 1: (10-15), and the repair extract obtained has a better effect.

[0045] In combination with Example 3, Example 6 and Example 7, it can be seen that when preparing the repair water, according to the raw material ratios in Example 3, Example 6 and Example 7, the repair effect of the repair water obtained is better.

[0046] Combining Example 6, Example 8 and Example 9, the repair effect of the repair water in Example 6 is the best. It can be seen that when the promoting composition is added, the promoting composition is preferably a mixture of allantoin, growth factor, hydroxy acid and repair extract, and the repair effect of the repair water obtained will be better.

[0047] Combining Example 6, Example 10 and Example 11, the repair effect of the repair water in Example 10 is the best. It can be seen that when an antioxidant is added, the antioxidant is preferably a mixture of magnesium ascorbyl phosphate and tocopherol, and the repair effect of the repair water obtained will be better.

[0048] Combining Example 6, Example 12 and Example 13, the repair effect of the repair water in Example 12 is the best. It can be seen that when preparing the repair water, the preparation conditions in Example 12 are the best, so the repair effect of the repair water is the best.

[0049] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A repair water containing recombinant humanized type III collagen, characterized by: The repair water is composed of the following raw materials in percentage by mass: 0.08-0.12% of recombinant humanized type III collagen, 0.13-0.18% of hydroxyethyl cellulose, 1.9-2.1% of propylene glycol, 0.08-0.12% of pentylene glycol, 0.3-0.4% of PE9010, 0.08-0.12% of the promoting composition, 0.1-0.12% of the antioxidant, 0.08-0.12% of cyclodextrin, and the remainder is purified water.

2. The repair water containing recombinant type III humanized collagen according to claim 1, characterized in that: The promoting composition comprises a mixture of allantoin, growth factors, hydroxy acids and repair extracts.

3. The repair water containing recombinant type III humanized collagen according to claim 2, characterized in that: The growth factor comprises one of epidermal growth factor and fibroblast growth factor.

4. The repair water containing recombinant type III humanized collagen according to claim 2, characterized in that: The hydroxy acid includes one or more of lactic acid, salicylic acid, and malic acid.

5. The repair water containing recombinant type III humanized collagen according to claim 2, characterized in that: The preparation of the repair extract comprises the following steps: Wash, cut and crush Panax notoginseng, Salvia miltiorrhiza, Radix Paeoniae Rubra, Radix Astragali and Cinnamomum cassia to obtain crushed materials; The crushed material is soaked in an ethanol aqueous solution with a concentration of 50%-70% for extraction, filtered, heated under reflux, and an extract is obtained; The extract is subjected to reduced pressure distillation to obtain a repair extract.

6. The repair water containing recombinant type III humanized collagen according to claim 5, characterized in that: The weight ratio of the crushed material to the ethanol aqueous solution is 1:(10-15).

7. The repair water containing recombinant type III humanized collagen according to claim 1, characterized in that: The antioxidant comprises a mixture of magnesium ascorbyl phosphate and tocopherol.

8. A method for preparing the repair water containing recombinant type III humanized collagen according to any one of claims 1 to 7, characterized in that: The following steps are involved: Weigh 5-6% of the total mass of the preparation water with purified water to dissolve the recombinant type III humanized collagen to form a uniform transparent solution to obtain solution A; use propylene glycol and pentylene glycol to pre-disperse hydroxyethyl cellulose to form a uniform suspension to obtain solution B; Weigh 78-80% of the total mass of the prepared water, add purified water to the preparation tank, stir and heat to 80-90°C, add solution B, reflux and cool, when the temperature drops to 55-65°C, add solution A, then add the remaining purified water of the total mass of the prepared water, continue stirring, and obtain a colorless transparent solution; When the temperature drops to 40-55°C, PE9010, the promoting composition, the antioxidant and the cyclodextrin are added, and the mixture is stirred while keeping the temperature to obtain the repair water.