Ultrasonic pipetting device with sealed end face

Abstract

The invention provides an ultrasonic pipetting device with a sealed end face, and belongs to the technical field of non-contact trace liquid transfer. The problems that an open type coupling liquid pool is adopted for existing ultrasonic pipetting, coupling liquid circulation is achieved through a pump body, and the pipetting precision is not high are solved. An ultrasonic pipetting device comprises a first source liquid pool, a second source liquid pool and an ultrasonic pipetting assembly, the ultrasonic pipetting assembly comprises an ultrasonic pipetting total base body, an ultrasonic transduction assembly, an end face sealing assembly and a coupling liquid adding assembly, the first source liquid pool is used for storing source liquid needing to be transferred, and the second source liquid pool is used for collecting the source liquid transferred from the first source liquid pool. The end face sealing assembly forms multi-layer sealing at the contact position of the ultrasonic pipetting total base body and the first source liquid pool. The device has the advantages of sealing coupling liquid, reducing turbulent flow and turbulent flow influence and reducing scattering and reflection of sound waves, thereby reducing energy attenuation of the sound waves and improving pipetting precision.

Description

Technical Field

[0001] The invention relates to the technical field of non-contact micro-liquid transfer, in particular to an end-face sealed ultrasonic liquid transfer device. Background Art

[0002] With the development of life sciences, chemical analysis, nanotechnology and other fields, the requirements for the accuracy, throughput and timeliness of liquid transfer are constantly increasing. Ultrasonic pipetting technology, as a non-contact pipetting method that uses ultrasonic sound fields to manipulate tiny droplets, can not only accurately control the volume of droplets to achieve the goal of automated pipetting, but also does not require the use of equipment such as pipetting heads to achieve non-contact liquid to avoid cross-infection of samples. With the advantages of non-contact, high degree of automation, precise pipetting, and high throughput, it has become the most popular pipetting method today.

[0003] Ultrasonic pipetting mainly uses a focusing transducer to achieve liquid transfer. Specifically, the focusing transducer generates ultrasonic waves that pass through the coupling liquid in the coupling liquid pool and then focus on the liquid surface of the source liquid pool, generating a sufficiently large acoustic radiation force at the focus to break through the surface tension of the excited droplets, generating micro-droplets to achieve the purpose of liquid transfer.

[0004] The coupling liquid pool used in the existing ultrasonic pipetting technology is open. During ultrasonic pipetting operations, the coupling liquid will be consumed due to the frequent movement of the equipment. If continuous pipetting operations are desired, the space between the focusing transducer and the source liquid pool must be filled with coupling liquid. Currently, a common method is to use a pump to continuously add coupling liquid to the coupling liquid pool and circulate the coupling liquid. However, the circulation of this coupling liquid between the focusing transducer and the source liquid pool will cause the ultrasonic wave to be affected by additional turbulence and turbulence during propagation. These disturbances will cause the scattering and reflection of the sound waves, resulting in faster energy attenuation, which in turn affects the pipetting accuracy of the ultrasonic pipetting. Summary of the invention

[0005] 1. Technical issues to be resolved In view of the deficiencies of the prior art, the present invention provides an end-face sealed ultrasonic pipetting device with a sealed coupling liquid, which reduces the influence of additional turbulence and turbulence, reduces the scattering and reflection of sound waves, thereby reducing the energy attenuation of sound waves and improving the precision of pipetting. The existing ultrasonic pipetting technology uses an open coupling liquid pool, which uses a pump body to continuously add coupling liquid to the coupling liquid pool and realize the circulation of the coupling liquid. However, the coupling liquid circulates between the focusing transducer and the source liquid pool, which will cause the ultrasonic wave to be affected by additional turbulence and turbulence during propagation. These disturbances will cause the scattering and reflection of sound waves, resulting in faster energy attenuation, which in turn affects the pipetting precision of the ultrasonic pipetting.

[0006] (II) Technical solution In order to achieve the above-mentioned sealed coupling liquid, reduce the influence of additional turbulence and turbulence, reduce the scattering and reflection of sound waves, thereby reduce the energy attenuation of sound waves and improve the pipetting accuracy, the present invention provides the following technical solutions: an ultrasonic pipetting device, comprising a first source liquid pool, a second source liquid pool and an ultrasonic pipetting component located below the first source liquid pool, the ultrasonic pipetting component comprising an ultrasonic pipetting total matrix, an ultrasonic transducer component, an end face sealing component and a coupling liquid adding component, the first source liquid pool and the second source liquid pool are fixed on a source plate bracket, the source plate bracket is a fixed structure, the first source liquid pool is used to store the source liquid to be transferred, the second source liquid pool is used to collect the source liquid transferred from the first source liquid pool, the coupling liquid adding component is used to apply pressure to the coupling liquid therein so that the coupling liquid enters the ultrasonic pipetting total matrix, and the end face sealing component forms a multi-level seal at the contact point between the ultrasonic pipetting total matrix and the first source liquid pool.

[0007] Preferably, source liquid cavities are provided inside the first source liquid pool and the second source liquid pool, and the source liquid cavities are arranged in an array.

[0008] Preferably, the ultrasonic pipetting main body is connected to the first source liquid pool through an end face sealing assembly, and the ultrasonic pipetting main body comprises a circular ring base, a coupling liquid pool, an outer ring adjustment bolt and an inner ring adjustment bolt, the circular ring base is used to install the end face sealing assembly and collect the lost coupling liquid, and the coupling liquid pool is used to install the ultrasonic transducer assembly and store the coupling liquid.

[0009] Preferably, the ultrasonic transducer assembly comprises an ultrasonic transducer, an ultrasonic transducer mounting base, an ultrasonic transducer fastening threaded rod, a base sealing ring 1 and a base sealing ring 2. The ultrasonic transducer assembly is installed in a coupling liquid pool and emits ultrasonic waves during ultrasonic pipetting to transfer the experimental reagents in the first source liquid pool to the second source liquid pool for collection.

[0010] Preferably, the end face sealing assembly includes an end face sealing ring, an outer sealing spring assembly and an inner sealing spring assembly. The end face sealing ring is installed between the first source liquid pool and the ultrasonic pipetting total matrix to form four seals A, B, C, and D between the first source liquid pool and the ultrasonic pipetting total matrix.

[0011] Preferably, the end face sealing ring comprises an inner sealing lip, an outer sealing lip and a coupling liquid collecting hole, the outer sealing spring assembly is pressed between the outer sealing lip and the annular base through an outer ring adjusting bolt, and the inner sealing spring assembly is pressed between the inner sealing lip and the annular base through an inner ring adjusting bolt; The end face sealing ring also includes an annular reinforcing sealing spring and an annular sealing part, the annular sealing part is attached to the top outer wall of the coupling liquid pool, and an annular reinforcing sealing spring is also arranged on the outer side of the annular sealing part. The contraction of the annular reinforcing sealing spring generates a horizontal force F on the annular sealing part, so that the annular sealing part and the coupling liquid pool form a seal at D; The end face sealing ring also includes a dust sealing lip, which is located on the outside of the outer sealing lip and forms a V-shape with the outer sealing lip. The dust sealing lip forms a seal with the lower surface of the first source liquid pool at point A under the pressure of the outer sealing spring assembly and the inner sealing spring assembly.

[0012] Preferably, the outer sealing spring assembly comprises an outer sealing upper spring seat, an outer sealing spring and an outer sealing lower spring seat, the outer sealing upper spring seat is embedded below the outer sealing lip, the outer sealing lower spring seat is arranged at a corresponding position of the lower annular base, and an outer sealing spring is installed between the outer sealing upper spring seat and the outer sealing lower spring seat; The inner sealing spring assembly includes an inner sealing upper spring seat, an inner sealing spring and an inner sealing lower spring seat. The inner sealing upper spring seat is embedded below the inner sealing lip, and the inner sealing lower spring seat is arranged at a corresponding position of the lower circular base. An inner sealing spring is installed between the inner sealing upper spring seat and the inner sealing lower spring seat.

[0013] Preferably, the inner sealing lip and the outer sealing lip are two concentric annular protrusions on the top of the end face sealing ring; When performing ultrasonic pipetting; The inner sealing spring assembly is adjusted to move vertically upward by the inner ring adjusting bolt, causing the inner sealing spring to be compressed, thereby generating a vertical upward force F1, so that the inner sealing lip and the lower surface of the first source liquid pool form a seal at B. The outer sealing spring assembly is adjusted to move vertically upward by the outer ring adjusting bolt, causing the inner outer sealing spring to be compressed, thereby generating a vertical upward force F2, so that the outer sealing lip and the lower surface of the first source liquid pool form a seal at C.

[0014] Preferably, the coupling liquid adding assembly comprises a connecting assembly, a sealed piston cylinder, a piston assembly and a pin shaft, the connecting assembly is used to connect the coupling liquid pool and the sealed piston cylinder, the piston assembly is used to apply pressure to the coupling liquid in the sealed piston cylinder so that the coupling liquid enters the coupling liquid pool, and the pin shaft is used to fix the piston assembly.

[0015] (III) Beneficial effects Compared with the prior art, the present invention provides an end-face sealed ultrasonic liquid transfer device, which has the following beneficial effects: The end-face sealed ultrasonic liquid transfer device firstly adjusts the inner ring adjustment bolt, and utilizes the elastic force of the inner sealing spring inside the inner sealing spring assembly to push the inner sealing lip and the lower surface of the first source liquid pool to form a seal at B, which is used to prevent the coupling liquid from directly leaking from the lower surface of the first source liquid pool. Then, by adjusting the outer ring adjustment bolt, the elastic force of the outer sealing spring inside the outer sealing spring assembly is used to push the outer sealing lip and the lower surface of the first source liquid pool to form a seal at C, which is used to collect and scrape the coupling liquid leaking from the inner sealing lip when the ultrasonic transducer assembly is moved. After the inner ring adjustment bolt and the outer ring adjustment bolt are adjusted in place, the dust sealing lip forms a seal at A with the lower surface of the first source liquid pool, which is used to prevent the external dust from contaminating the coupling liquid in the coupling liquid pool. At the contact point D between the end face sealing ring and the ultrasonic liquid transfer total base, pressure is applied to the annular seal of the end face sealing ring by the contraction force of the annular reinforcing sealing spring to form a seal at D, which is used to prevent the coupling liquid from leaking from the contact point. This achieves the effect of sealing the coupling liquid, reducing the influence of turbulence and turbulence, reducing the scattering and reflection of sound waves, thereby reducing the energy attenuation of sound waves and improving the accuracy of pipetting. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the end face sealing and coupling liquid adding structure of an end face sealed ultrasonic liquid transfer device of the present invention; Figure 2 This is a schematic structural diagram of an ultrasonic liquid transfer device of the present invention; Figure 3 This is a schematic structural diagram of an end face sealing assembly of an ultrasonic liquid transfer device of the present invention; Figure 4 This is a working diagram of a coupling liquid adding component of an ultrasonic liquid transfer device of the present invention; Figure 5 The present invention is a schematic structural diagram of an ultrasonic transducer assembly of an ultrasonic liquid transfer device.

[0017] In the figure: 1. a first source liquid pool; 11. a source liquid chamber; 2. End face sealing assembly; 21. End face sealing ring; 2101. External sealing lip; 2102. Internal sealing lip; 2103. Annular reinforcing sealing spring; 2104. Annular sealing place; 2105. Metal skeleton; 2106. Coupling liquid collecting hole; 2107. Dust sealing lip; 22. External sealing spring assembly; 2201. External sealing upper spring seat; 2202. External sealing spring; 2203. External sealing lower spring seat; 23. Internal sealing spring assembly; 2301. Internal sealing upper spring seat; 2302. Internal sealing spring; 2303. Internal sealing lower spring seat; 3. Ultrasonic pipetting main body; 31. Circular ring base; 32. Coupling liquid pool; 33. Outer ring adjustment bolt; 34. Inner ring adjustment bolt; 4. Ultrasonic transducer assembly; 41. Ultrasonic transducer; 42. Ultrasonic transducer mounting base; 43. Ultrasonic transducer fastening threaded rod; 44. Base sealing ring 1; 45. Base sealing ring 2; 5. Coupling fluid adding assembly; 51. Connecting assembly; 5101. Stop valve; 5102. Connecting hose; 52. Sealing piston cylinder; 53. Piston assembly; 5301. Piston sealing ring; 5302. Pressurizing piston; 54. Pin shaft; 6. The second source liquid pool. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-5 An ultrasonic liquid transfer device includes a first source liquid pool 1 (for storing experimental source liquid), a second source liquid pool 6 (for receiving experimental source liquid) located at a corresponding position above the first source liquid pool 1, and an ultrasonic liquid transfer component located below the first source liquid pool 1. The ultrasonic liquid transfer component also includes an end face sealing component 2, an ultrasonic liquid transfer matrix 3, an ultrasonic transducer component 4, and a coupling liquid adding component 5. The coupling liquid adding component 5 applies pressure to the coupling liquid contained therein so that the coupling liquid enters the ultrasonic liquid transfer matrix 3 to achieve the transfer of the coupling liquid. The end face sealing component 2 forms a multi-layer seal at the contact point between the ultrasonic liquid transfer matrix 3 and the first source liquid pool 1. In this patent, there are four layers, namely A, B, C, and D, to prevent the coupling liquid from leaking from the contact point between the ultrasonic liquid transfer matrix 3 and the first source liquid pool 1.

[0020] The source liquid cavities 11 in the first source liquid pool 1 and the second source liquid pool 6 are mostly arranged in an array, and the side walls of the source liquid cavities 11 are inclined at a certain angle to the bottom surface. While accommodating as much source liquid as possible, the transfer and collection of the source liquid are also facilitated. The first source liquid pool 1 and the second source liquid pool 6 are fixed on a source plate bracket (not shown in the figure). The source plate bracket is a fixed structure, so the first source liquid pool 1 and the second source liquid pool 6 are also fixed at the set position.

[0021] See also Figure 2 and Figure 5The ultrasonic pipetting base 3 is an integrated structure, including a circular ring base 31, a coupling liquid pool 32, an outer ring adjustment bolt 33 and an inner ring adjustment bolt 34. There are four outer ring adjustment bolts 33 and four inner ring adjustment bolts 34, which are installed on the circular ring base 31 and used in conjunction with the outer sealing spring assembly 22 and the inner sealing spring assembly 23 respectively. The circular ring base 31 is a space separated by the outer wall of the ultrasonic pipetting base 3 and the coupling liquid pool 32 for installing the end face sealing assembly 2 to ensure that the end face sealing can be accurately adjusted, and the coupling liquid pool 32 is a cylindrical cavity connected from top to bottom, which is filled with coupling liquid and is also the installation site of the ultrasonic transducer assembly 4 Therefore, an annular groove is formed on the contact surface between the ultrasonic transducer mounting base 42 of the ultrasonic transducer assembly 4 and the coupling liquid pool 32, and a base sealing ring 2 45 is provided. The base sealing ring 2 45 is deformed by the extrusion of the ultrasonic transducer assembly 4 and the coupling liquid pool 32, and the contact between the ultrasonic transducer assembly 4 and the coupling liquid pool 32 is sealed to prevent leakage of the coupling liquid. An annular groove is formed on the contact surface between the ultrasonic transducer mounting base 42 of the ultrasonic transducer assembly 4 and the ultrasonic transducer 41, and a base sealing ring 1 44 is provided. The base sealing ring 1 44 is used to fix the ultrasonic transducer 41 with a certain strength, so that it can move axially in the ultrasonic transducer mounting base 42. The ultrasonic transducer assembly 4 can move axially in the coupling liquid pool 32 to adjust the distance from the ultrasonic transducer 41 to the lower surface of the first source liquid pool 1, and then adjust the focal length. When the ultrasonic transducer assembly 4 performs ultrasonic pipetting, ultrasonic waves are emitted to transfer the experimental reagents in the first source liquid pool 1 to the second source liquid pool 6 for collection.

[0022] See also Figures 1 to 3 The end face sealing assembly 2 is installed between the first source liquid pool 1 and the ultrasonic pipetting main base 3. The end face sealing assembly 2 includes an end face sealing ring 21, an outer sealing spring assembly 22 and an inner sealing spring assembly 23. The end face sealing ring 21 is made of rubber. The metal skeleton 2105 installed inside provides rigid support for the overall shape of the end face sealing ring 21, ensuring that the end face sealing ring 21 maintains its designed shape and size during installation and use, and is not easily deformed. The dust sealing lip 2107, the outer sealing lip 2101 and the inner sealing lip 2102 in the end face sealing ring 21 have three contact points A, B, and C with the lower surface of the first source liquid pool 1 respectively. The annular sealing point 2104 in the end face sealing ring 21 contacts the outer wall of the coupling liquid pool 32 at D. The end face sealing ring 21 contacts the lower surface of the first source liquid pool 1 and the outer wall of the coupling liquid pool 32 at A, B, C, and D to produce four seals.

[0023] The seal at point B is used to prevent the coupling liquid in the coupling liquid pool 32 from leaking from the lower surface of the first source liquid pool 1. To achieve the seal at point B, the inner sealing lip 2102, the inner sealing spring assembly 23 and the inner ring adjustment bolt 34 need to cooperate with each other. First, adjust the four inner ring adjustment bolts 34 clockwise respectively. The inner ring adjustment bolts 34 are installed in the threaded holes of the annular base 31 and are evenly distributed directly below the inner seal lower spring seat 2303. When the inner ring adjustment bolts 34 are adjusted clockwise, the inner ring adjustment bolts 34 will move axially along the threaded holes of the annular base 31, thereby pushing the inner seal lower spring seat 2303 to vertically compress the inner seal spring 2302. The inner seal spring 2302 is compressed and elastic force is generated at both ends. Due to the transfer of force, the elastic force generated by the inner seal spring 2302 is vertically transmitted to the inner seal upper spring seat 2301 to form a force F1, and the inner seal upper spring seat 2301 will push the inner sealing lip 2102 to move vertically upward due to the force F1. However, the first source liquid pool 1 is fixedly installed in a certain position, so the inner sealing lip 2102 is elastically deformed at point B due to the force F1 and forms a seal at point B on the lower surface of the first source liquid pool 1.

[0024] Since the source liquid chambers 11 of the first source liquid pool 1 are arranged in an array, and the ultrasonic pipetting operation requires the experimental source liquid in the source liquid chambers 11 arranged in the array to be pipetted in sequence, the end face sealing component 2 also needs to be moved on the lower surface of the first source liquid pool. During the movement of the end face sealing component 2, the seal may become loose, causing the coupling liquid to leak from the B seal, thereby affecting the sealing effect and overall performance of the system. In addition, there will be coupling liquid residue at the original coupling position of the first source liquid pool 1 and the coupling liquid pool 32. In order to collect the coupling liquid leaked at the B seal and the residual coupling liquid on the lower surface of the first source liquid pool 1, a secondary seal, that is, the seal at C, is required.

[0025] To achieve sealing at point C, the outer sealing lip 2101, the outer sealing spring assembly 22 and the outer ring adjustment bolt 33 need to work together. Adjust the four outer ring adjustment bolts 33 clockwise respectively. The outer ring adjustment bolts 33 are installed in the threaded holes of the annular base 31 and are evenly distributed directly below the outer seal lower spring seat 2203. When the outer ring adjustment bolts 33 are adjusted clockwise, the outer ring adjustment bolts 33 will move axially along the threaded holes of the annular base 31, thereby pushing the outer seal lower spring seat 2203 to vertically compress the outer seal spring 2202. The outer seal spring 2202 is compressed and elastic force is generated at both ends. Due to the transfer of force, the elastic force generated by the outer seal spring 2202 is vertically transmitted to the outer seal upper spring seat 2201 to form a force F2, and the outer seal upper spring seat 2201 will push the outer sealing lip 2101 to move vertically upward due to the force F2. However, the first source liquid pool 1 is fixedly installed in a certain position, so the outer sealing lip 2101 is elastically deformed at C due to the force F2 and forms a seal at C with the lower surface of the first source liquid pool 1.

[0026] Among them, there is a trapezoidal cavity between the seal at B and the seal at C. When the coupling liquid leaks from the seal at B, due to the secondary seal of the seal at C, the coupling liquid will flow through the inclined surface of the trapezoidal cavity to the bottom, and the coupling liquid remaining on the lower surface of the first source liquid pool 1 will be scraped by the outer sealing lip of the seal at C, and flow through the inclined surface of the trapezoidal cavity to the bottom. A coupling liquid collection hole 2106 is opened at the bottom of the trapezoidal cavity. The coupling liquid in the trapezoidal cavity will flow into the annular base 31 through the coupling liquid collection hole 2106 and be finally collected.

[0027] Since the forces F1 and F2 act on the end face sealing ring 21 at B and C respectively, the end face sealing ring 21 is deformed in the V-shaped area between C and A. Due to the deformation, the dust sealing lip 2107 of the end face sealing ring 21 is fitted with the lower surface of the first source liquid pool 1 at A, thereby sealing and preventing dust in the external environment from contaminating the coupling liquid in the coupling liquid pool 32.

[0028] The end face sealing ring 21 and the coupling liquid pool 32 are still in contact at D. To prevent the coupling liquid from leaking at the contact point D, it is necessary to seal at D. An annular reinforcing sealing spring 2103 is installed at D. The annular reinforcing sealing spring 2103 will generate a horizontal force F3 on the contact surface between the annular sealing part 2104 of the end face sealing ring 21 and the coupling liquid pool 32 due to its own contraction. The force F3 will squeeze the outer surface of the annular sealing part 2104 and the coupling liquid pool 32, thereby forming a seal at D.

[0029] In this patent, the sizes of F1 and F2 can be adjusted by adjusting the axial displacement of the outer ring adjustment bolt 33 and the inner ring adjustment bolt 34 in the threaded hole to adjust the compression degree of the outer sealing spring 2202 and the inner sealing spring 2302. In addition, the sizes of F1, F2 and F3 can be further adjusted by using the inner sealing spring 2302, the outer sealing spring 2202 and the annular reinforcing sealing spring 2103 with different elastic coefficients (stiffness coefficients). The sealing effect of the end face sealing ring 21 at the four positions A, B, C and D is closely related to the sizes of F1, F2 and F3. Therefore, in this patent, the sizes of F1, F2 and F3 can be flexibly adjusted according to different experimental conditions, thereby optimizing the end face sealing effect and enabling the end face sealing assembly 2 to adapt to a variety of experimental environments.

[0030] See also Figure 4, is a flowchart of the working process of the coupling liquid adding component 5, which includes a connecting component 51, a sealing piston cylinder 52, a piston component 53 and a pin 54. The connecting component 51 includes a connecting hose 5102 and a stop valve 5101, and the stop valve 5101 is installed on the connecting hose 5102. The piston component 53 includes a pressurizing piston 5302, which is installed in the sealing piston cylinder 52. The outer surface of the pressurizing piston 5302 is provided with an annular groove and a piston sealing ring 5301 is installed. The piston sealing ring 5301 is used for the installation and sealing of the piston component 53 and the sealing piston cylinder 52.

[0031] When the coupling liquid adding component 5 is in working state, the stop valve 5101 is opened, the pin 54 is pulled out to ensure that the piston component 53 is in a free state, and the piston component 53 will move axially downward in the sealed piston cylinder 52 due to its own gravity, thereby generating pressure on the coupling liquid in the sealed piston cylinder 52. Because the coupling liquid is incompressible, the coupling liquid will release its own pressure by flowing when it is under pressure. Therefore, the coupling liquid will be transmitted to the coupling liquid pool 32 through the connecting hose 5102 of the connecting component 51 until the coupling liquid pool 32 is filled with coupling liquid. When the coupling liquid adding component 5 is not working, the stop valve 5101 is closed, the pin 54 is inserted into the piston component 53, and the piston component 53 is fixed to the sealed piston cylinder 52 by the pin 54. At this time, the piston component 53 will not generate pressure on the coupling liquid in the sealed piston cylinder 52, and the coupling liquid will be stored in the sealed piston cylinder 52. Among them, the pressure generated by the piston assembly 53 can be adjusted by replacing the piston assembly 53 of different masses, thereby adjusting the pressure applied by the piston assembly 53 to the coupling liquid. This pressure adjustment mechanism ensures that the coupling liquid adding component 5 can adapt to a variety of ultrasonic pipetting coupling working scenarios and meet the needs under different experimental conditions.

[0032] Here is how to use the device: First, by adjusting the inner ring adjusting bolt 34, the elastic force of the inner sealing spring 2302 inside the inner sealing spring assembly 23 is used to push the inner sealing lip 2101 and the lower surface of the first source liquid pool 1 to form a seal at B, so as to prevent the coupling liquid from directly leaking from the lower surface of the first source liquid pool 1; Then, by adjusting the outer ring adjusting bolt 33, the elastic force of the outer sealing spring 2202 inside the outer sealing spring assembly 22 is used to push the outer sealing lip 2101 and the lower surface of the first source liquid pool 1 to form a seal at C, which is used to collect and scrape and prevent the coupling liquid leaking from the inner sealing lip 2102 when the ultrasonic transducer assembly 4 is moved, and at the same time cooperates with the seal at B to collect the leaked coupling liquid; After the inner ring adjusting bolt 34 and the outer ring adjusting bolt 33 are adjusted in place, the dust sealing lip 2107 forms a seal at A with the lower surface of the first source liquid pool 1 to prevent external dust from contaminating the coupling liquid in the coupling liquid pool 32; At the contact point D between the end face sealing ring 21 and the ultrasonic pipetting main base 3, pressure is applied to the annular sealing point 2104 of the end face sealing ring 21 through the contraction force of the annular reinforcing sealing spring 2103 to form a seal at D to prevent the coupling liquid from leaking from the contact point.

[0033] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ultrasonic liquid transfer device, characterized in that: The invention comprises a first source liquid pool (1), a second source liquid pool (6) and an ultrasonic liquid transfer component located below the first source liquid pool (1). The ultrasonic liquid transfer component comprises an ultrasonic liquid transfer matrix (3), an ultrasonic transducer component (4), an end face sealing component (2) and a coupling liquid adding component (5). The first source liquid pool (1) and the second source liquid pool (6) are fixed on a source plate support, and the source plate support is a fixed structure. The first source liquid pool (1) is used to store source liquid to be transferred, and the second source liquid pool (6) is used to collect source liquid transferred from the first source liquid pool (1). The coupling liquid adding component (5) is used to apply pressure to the coupling liquid therein so that the coupling liquid enters the ultrasonic liquid transfer matrix (3). The end face sealing component (2) forms a multi-layer seal at the contact point between the ultrasonic liquid transfer matrix (3) and the first source liquid pool (1).

2. The ultrasonic liquid transfer device according to claim 1, characterized in that: Both the first source liquid pool (1) and the second source liquid pool (6) are provided with source liquid cavities (11) therein, and the source liquid cavities (11) are arranged in an array.

3. The ultrasonic liquid transfer device according to claim 1, characterized in that: The ultrasonic liquid transfer overall base (3) is connected to the first source liquid pool (1) via an end face sealing component (2). The ultrasonic liquid transfer overall base (3) comprises a circular ring-shaped base (31), a coupling liquid pool (32), an outer ring adjustment bolt (33) and an inner ring adjustment bolt (34). The circular ring-shaped base (31) is used to install the end face sealing component (2) and collect lost coupling liquid, and the coupling liquid pool (32) is used to install the ultrasonic transducer component (4) and store the coupling liquid.

4. The ultrasonic liquid transfer device according to claim 1, characterized in that: The ultrasonic transducer assembly (4) comprises an ultrasonic transducer (41), an ultrasonic transducer mounting base (42), an ultrasonic transducer fastening threaded rod (43), a base sealing ring 1 (44) and a base sealing ring 2 (45). The ultrasonic transducer assembly (4) is installed in a coupling liquid pool (32) and emits ultrasonic waves when ultrasonic pipetting is performed to transfer the experimental reagent in the first source liquid pool (1) to the second source liquid pool (6) for collection.

5. The ultrasonic liquid transfer device according to claim 1, characterized in that: The end face sealing assembly (2) comprises an end face sealing ring (21), an outer sealing spring assembly (22) and an inner sealing spring assembly (23); the end face sealing ring (21) is installed between the first source liquid pool (1) and the ultrasonic liquid transfer overall matrix (3), and forms four seals A, B, C and D between the first source liquid pool (1) and the ultrasonic liquid transfer overall matrix (3).

6. The ultrasonic liquid transfer device according to claim 5, characterized in that: The end face sealing ring (21) comprises an inner sealing lip (2102), an outer sealing lip (2101) and a coupling liquid collecting hole (2106); the outer sealing spring assembly (22) is pressed between the outer sealing lip (2101) and the annular base (31) via an outer ring adjusting bolt (33); and the inner sealing spring assembly (23) is pressed between the inner sealing lip (2102) and the annular base (31) via an inner ring adjusting bolt (34); The end face sealing ring (21) further comprises an annular reinforcing sealing spring (2103) and an annular sealing portion (2104); the annular sealing portion (2104) is fitted onto the top outer wall of the coupling liquid pool (32); an annular reinforcing sealing spring (2103) is further arranged on the outer side of the annular sealing portion (2104); the annular reinforcing sealing spring (2103) contracts to generate a horizontal force F3 on the annular sealing portion (2104), so that the annular sealing portion (2104) and the coupling liquid pool (32) form a seal at D; The end face sealing ring (21) further comprises a dust sealing lip (2107), wherein the dust sealing lip (2107) is located on the outside of the outer sealing lip (2101), and a V-shape is formed between the dust sealing lip (2107) and the outer sealing lip (2101). The dust sealing lip (2107) forms a seal with the lower surface of the first source liquid pool (1) at point A under the abutment of the outer sealing spring assembly (22) and the inner sealing spring assembly (23).

7. The ultrasonic liquid transfer device according to claim 5, characterized in that: The outer sealing spring assembly (22) comprises an outer sealing upper spring seat (2201), an outer sealing spring (2202) and an outer sealing lower spring seat (2203), wherein the outer sealing upper spring seat (2201) is embedded below the outer sealing lip (2101), and the outer sealing lower spring seat (2203) is arranged at a corresponding position of the annular base (31) below, and the outer sealing spring (2202) is installed between the outer sealing upper spring seat (2201) and the outer sealing lower spring seat (2203); The inner sealing spring assembly (23) comprises an inner sealing upper spring seat (2301), an inner sealing spring (2302) and an inner sealing lower spring seat (2303), wherein the inner sealing upper spring seat (2301) is embedded below the inner sealing lip (2102), and the inner sealing lower spring seat (2303) is arranged at a corresponding position of the circular ring base (31) below, and the inner sealing spring (2302) is installed between the inner sealing upper spring seat (2301) and the inner sealing lower spring seat (2303).

8. The ultrasonic liquid transfer device according to claim 6, characterized in that: The inner sealing lip (2102) and the outer sealing lip (2101) are two concentric annular protrusions on the top of the end face sealing ring (21); When performing ultrasonic pipetting; The inner sealing spring assembly (23) is adjusted to move vertically upwards by means of the inner ring adjusting bolt (34), causing the inner sealing spring (2302) inside to be compressed, thereby generating a vertically upward force F1, so that the inner sealing lip (2102) and the lower surface of the first source liquid pool (1) form a seal at point B. The outer sealing spring assembly (22) is adjusted to move vertically upwards by means of an outer ring adjusting bolt (33), causing the inner outer sealing spring (2202) to be compressed, thereby generating a vertically upward force F2, so that the outer sealing lip (2101) and the lower surface of the first source liquid pool (1) form a seal at point C.

9. The ultrasonic liquid transfer device according to claim 1, characterized in that: The coupling liquid adding component (5) comprises a connecting component (51), a sealing piston cylinder (52), a piston component (53) and a pin shaft (54); the connecting component (51) is used to connect the coupling liquid pool (32) and the sealing piston cylinder (52); the piston component (53) is used to apply pressure to the coupling liquid in the sealing piston cylinder (52) so that the coupling liquid enters the coupling liquid pool (32); and the pin shaft (54) is used to fix the piston component (53).

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