Ganoderma lucidum peptide extraction and separation equipment and method for anti-tumor and immunity-enhancing

Abstract

The invention relates to the technical field of extraction and separation equipment, and discloses an anti-tumor and immunity-enhancing ganoderma peptide extraction and separation equipment, comprising a kettle body, wherein a detection and collection component is fixedly installed at the bottom of the kettle body, and the detection and collection component comprises a mass spectrometer detector, a fraction collector and a waste liquid recovery device. The anti-tumor and immunity-enhancing ganoderma peptide extraction and separation equipment and method thereof are characterized in that microcapsules are arranged at the bottom of the kettle body, an electromagnetic coil is embedded in the bottom of the kettle body, and an enzymolysis agent is released in stages by adjusting the magnetic field intensity, so as to avoid self-degradation caused by excessive initial concentration. In addition, a main ultrasonic transducer with a two-layer structure is arranged inside, and the frequencies of the upper and lower sides are different, so that the extraction and separation equipment adopts ultrasonic enzymolysis synergistic effect to extract ganoderma peptide, realizes that ultrasonic cavitation effect and enzymolysis reaction are carried out synchronously, and significantly improves the ganoderma cell wall crushing efficiency and peptide release rate.

Description

Technical Field

[0001] The invention relates to the technical field of extraction and separation equipment, in particular to an extraction and separation equipment and method for a ganoderma lucidum peptide capable of resisting tumors and improving immunity. Background Art

[0002] Lingzhi peptides are a class of small molecule active peptides extracted from Lingzhi, which have multiple biological activities and medicinal values. Lingzhi, as a traditional Chinese medicinal material, has a history of application for thousands of years. With the development of modern science and technology, the chemical composition and pharmacological effects of Lingzhi have been deeply studied. Studies have found that Lingzhi contains a variety of active ingredients, including polysaccharides, triterpenoid compounds, proteins and peptides, and the discovery and research of Lingzhi peptides are the results of the combination of modern biotechnology and traditional Chinese medicine. Its molecular weight is small and easy to be absorbed by the human body. It has the effects of enhancing the activity of immune cells, improving the body's immunity, inhibiting the growth of tumor cells, and inducing tumor cell apoptosis. Therefore, it is a crucial step to be able to extract Lingzhi peptides well. The existing extraction and separation equipment needs to be extracted, separated and purified in multiple steps and multiple equipment. The equipment occupies a large area and has low efficiency. In addition, the separation technology is relatively simple, and it is difficult to achieve accurate classification of peptides of different molecular weights, resulting in poor separation effect. For this reason, we propose an anti-tumor and immunity-enhancing Lingzhi peptide extraction and separation equipment and method thereof. Summary of the invention

[0003] The present invention provides an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device and method thereof, which has the advantages of ultrasonic enzymatic hydrolysis synergistic extraction, good separation and filtration effect, and high equipment integration, and solves the problems raised in the above-mentioned background technology.

[0004] The present invention provides the following technical solution: an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a kettle body, a detection and collection component fixedly installed at the bottom end of the kettle body, the detection and collection component comprising a mass spectrometer detector, a fraction collector and a waste liquid recovery device, a sliding ring movably installed at the top end of the detection and collection component, a microcapsule is arranged at the bottom end of the kettle body, an external motor is fixedly installed at the middle of the bottom end of the kettle body, an output shaft of the external motor passes through the bottom end of the kettle body and a central rod is fixedly installed, a stirring blade is fixedly installed circumferentially on the central rod, a top cover is movably installed at the middle of the top end of the kettle body, a feeding pipe is fixedly installed at the top end of the kettle body, and a stirring blade is fixedly installed at the top end of the kettle body. A partition plate is installed, a base is fixedly installed at the middle of the top end of the top cover, a sample introduction component is fixedly installed at the top end of the base, a straight groove is opened on the top cover, a vortex guide plate is fixedly installed inside the kettle body, an outer sleeve is fixedly installed on the sliding ring, a centrifugal separation component is movably installed on the outer sleeve, an external transmission gear is fixedly installed on the top end of the centrifugal separation component, an external drive component is fixedly installed on the side of the base, an ultrafiltration separation component is fixedly installed on the outside of the kettle body, a main ultrasonic transducer is annularly arranged inside the kettle body, and the sample introduction component and the ultrafiltration separation component, as well as the ultrafiltration separation component and the bottom end of the kettle body are connected by a high-pressure pipeline; The centrifugal separation assembly comprises a connecting straight rod, the upper and lower ends of the connecting straight rod are respectively fixedly mounted with outer ends, a centrifugal spiral tube is fixedly connected between the two outer ends, a universal joint is fixedly mounted at one end of the outer end, an external tube is fixedly mounted at the end of the universal joint, and an inner hose is provided inside the universal joint to connect the outer end and the external tube; The external driving component comprises an electric telescopic rod, a base rod is fixedly mounted on the end of the electric telescopic rod, a stepping motor is fixedly mounted on the bottom end of the base rod, and a driving gear is fixedly mounted on the output shaft of the stepping motor.

[0005] In a preferred embodiment, the bottom end of the sliding ring is embedded in the top end of the detection and collection component for sealing and rotation, and the edge coverage of the sliding ring exceeds the top edge of the detection and collection component. A plurality of protruding short rods are arranged on the side of the sliding ring and the short rods are slidably arranged against the bottom surface of the kettle body.

[0006] In a preferred embodiment, the microcapsule is designed in a magnetically controlled release manner, the protease is encapsulated in a temperature-sensitive chitosan capsule body, and the surface of the capsule body is coated with FeO nanoparticles, and an electromagnetic coil is embedded in the inner bottom of the kettle body.

[0007] In a preferred embodiment, the bottom end of the center rod passes through the bottom end of the kettle body and is rotatably arranged, and the top end thereof is located at the bottom end of the top cover and is fixedly connected, the diameter covered by the stirring blade is one third of the inner diameter of the kettle body, and a pitch-blade turbine structure is adopted, the surface of the stirring blade is coated with a polytetrafluoroethylene coating with a thickness of 50-100 μm, and a miniature ultrasonic transducer is embedded inside.

[0008] In a preferred embodiment, the top cover is embedded in the top of the kettle body and is rotatably arranged. A convex ring is arranged on the outer side of the top cover and the convex ring is movably clamped on the top of the kettle body. There are multiple partition plates and they are evenly located in a ring shape and fixed inside the kettle body. The vortex guide plate is spiral and extends upward from the bottom end around the inner wall of the kettle body. The width of the vortex guide plate is one-tenth of the kettle body, and its spiral pitch is one-fifth of the height of the kettle body. The cross-section of the vortex guide plate adopts an airfoil structure design, and it is made of stainless steel and coated with polytetrafluoroethylene on the surface. Reinforcing ribs are also welded on the back side. The vortex guide plate is fixed to the inner wall of the kettle body by bolts through an L-shaped bracket and is spaced apart from the inside of the kettle body, and the spacing distance is 10%-20% of the width of the guide plate. The partition plate is located in the above-mentioned interval and fixedly installed, so that the vortex guide plate and the inside of the kettle body form multiple flow channels.

[0009] In a preferred embodiment, a microfluidic chip is arranged in the external tube at the bottom, and a micro-heating plate is arranged at the bottom, a main channel and a branch channel are arranged on it, and an electrode array is arranged at the intersection of the two channels, the main channel connects the centrifugal spiral tube and the mass spectrometer detector and the waste liquid recovery device in the detection collection component, the branch channel is connected to the independent collection pool of the fraction collector, the outer sleeve of the external tube at the bottom end is relatively rotatable, and is fixedly connected to the outer sleeve at the top end, an extension ring is arranged on the outside of the external tube, and a plurality of evenly arranged leakage grooves are opened on the outer sleeve.

[0010] In a preferred embodiment, one end of the electric telescopic rod is fixedly mounted on a base, a circular hole is opened at the end of the base rod, a metal round tube is sealingly and rotatably mounted on the top end of the external transmission gear, and is fixedly connected to the outer sleeve, one end of the metal round tube passes through the external transmission gear and is connected to the outer sleeve and the outer end head, and the other end is connected to the injection assembly, the injection assembly includes an injection pump, a mobile phase mixer and an eight-way valve, the base rod is sleeved on the metal round tube, the drive gear is meshed with the external transmission gear and has a thickness less than that of the external transmission gear, and the external transmission gear is movably arranged in the straight groove.

[0011] In a preferred embodiment, the ultrafiltration separation component adopts a three-stage ultrafiltration membrane stacking design in series to form a multi-layer gradient membrane group. The membrane material is polyethersulfone, and each stage of the membrane component is externally connected to a semiconductor refrigeration plate. The temperature is adjusted by thermocouple feedback. A high-pressure pulse valve is arranged downstream of the ultrafiltration membrane to periodically backwash the membrane surface. Bionic leaf vein patterns are etched on the surface of the ultrafiltration membrane. Shape memory alloy is embedded in the interlayer of the membrane component. The shape memory alloy automatically adjusts the membrane surface tension according to temperature changes. One end of the ultrafiltration separation component is connected to the interior of the kettle body through a conduit.

[0012] In a preferred embodiment, the main ultrasonic transducers are arranged on upper and lower sides inside the kettle body, with a low-frequency transducer arranged on the upper layer and a high-frequency transducer arranged on the lower layer to form a superimposed sound field.

[0013] A ganoderma lucidum peptide extraction and separation device for anti-tumor and immunity enhancement, the specific use method and steps are as follows: S1. Equipment startup self-test and automatic loading of process parameters; S2, transporting the raw materials to the interior of the kettle through the feed pipe for raw material processing; S3, start the main ultrasonic transducer and adjust the parameters, connect the electromagnetic coil at the bottom of the kettle body and adjust the magnetic field strength as needed, and start the detection and collection assembly to drive the central rod to rotate as needed, so that the central rod drives the stirring blades and the top cover to rotate, and at the same time adjust the overall inclination angle of the centrifugal separation assembly through the external drive assembly, and the centrifugal separation assembly can also be driven to rotate by the external drive assembly during the rotation process; S4. The raw materials after enzymatic hydrolysis are filtered and separated by an ultrafiltration separation component, and the peptides that do not meet the requirements are returned to the kettle body for secondary enzymatic hydrolysis. The peptides that meet the requirements enter the centrifugal spiral tube through the injection component, and then are separated and collected by the microfluidic chip at the bottom. At the same time, the mass spectrometer is monitored in real time and the various parameters of the equipment are dynamically adjusted according to the monitoring results; S5. The collected purified liquid is subjected to subsequent concentration and drying processes to complete the final extraction and separation operation.

[0014] The present invention has the following beneficial effects: 1. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation equipment and method thereof are characterized in that microcapsules are arranged at the bottom end of the kettle body, an electromagnetic coil is embedded in the bottom of the kettle body, and the enzymolysis agent is released in stages by adjusting the magnetic field strength to avoid self-degradation caused by excessive initial concentration. In addition, the main ultrasonic transducer with a two-layer structure arranged inside is combined, and the main ultrasonic transducer of each layer is evenly distributed in a ring shape, and the frequencies on the upper and lower sides are different. The extraction and separation equipment adopts the synergistic effect of ultrasonic enzymolysis to extract the Ganoderma lucidum peptide, and the ultrasonic cavitation effect and the enzymolysis reaction are carried out simultaneously, which significantly improves the Ganoderma lucidum cell wall crushing efficiency and peptide release rate. At the same time, the ultrafiltration separation component adopts a multi-layer gradient ultrafiltration membrane group, and each membrane group is equipped with an independent temperature control system to realize ultrafiltration under low temperature conditions, reduce the thermal denaturation of peptides, and realize the preliminary classification of peptide segments by gradient retention, which is convenient for the subsequent fine extraction and separation of peptide segments and ensures the extraction and separation effect of high equipment.

[0015] 2. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation equipment and method thereof are provided with a centrifugal separation component inside the kettle body, and the outlet at the bottom of the centrifugal separation component is directly connected to a mass spectrometer through an electrospray ionization interface to collect mass spectrometry signals in real time. The mass spectrometry data is analyzed by an edge computing module to dynamically adjust the mobile phase ratio and flow rate. During use, the centrifugal separation component can realize the combination of rotation and revolution through the drive of an external drive component and an external motor respectively. The centrifugal separation component is a structural setting for adjusting the inclination angle that can be used in conjunction with the external drive component to realize the use of centrifugal force to accelerate the stratification of the two phases, the light phase gathers inwardly, and the heavy phase moves outwardly. A vortex guide plate is also provided inside to ensure that it realizes efficient vortex field generation and material mixing in the ultrasonic and enzymatic hydrolysis coordinated reactor. At the same time, the vortex guide plate and the inner wall of the kettle body are spaced apart, so that a certain flow channel is formed between the vortex guide plate and the inner wall of the kettle body, thereby optimizing the generation of the vortex field and the material mixing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the first three-dimensional structure of the present invention; Figure 2 It is a schematic diagram of the second three-dimensional structure of the present invention; Figure 3 It is a schematic diagram of a partial cross-sectional structure of the present invention; Figure 4 It is a schematic diagram of the partial internal three-dimensional structure of the present invention; Figure 5 It is a three-dimensional schematic diagram of the local structure of the centrifugal separation component of the present invention; Figure 6 It is a three-dimensional schematic diagram of the top local structure of the present invention.

[0017] In the figure: 1, kettle body; 2, detection and collection component; 3, sliding ring; 4, microcapsule; 5, external motor; 6, center rod; 7, stirring blade; 8, top cover; 9, feed pipe; 10, partition plate; 11, base; 12, sample injection component; 13, straight groove; 14, vortex guide plate; 15, centrifugal separation component; 151, connecting straight rod; 152, outer end; 153, centrifugal spiral tube; 154, universal joint; 155, external tube; 156, inner hose; 16, outer sleeve; 17, external transmission gear; 18, external drive component; 181, electric telescopic rod; 182, base rod; 183, stepping motor; 184, driving gear; 19, ultrafiltration separation component; 20, main ultrasonic transducer. DETAILED DESCRIPTION

[0018] The technical scheme of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the present invention. In addition, the forms of the various structures described in the following embodiments are only examples. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation equipment and method involved in the present invention are not limited to the various structures described in the following embodiments. All other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of the present invention.

[0019] See also Figure 1-4 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a kettle body 1, a detection and collection component 2 is fixedly installed at the bottom of the kettle body 1, the detection and collection component 2 includes a mass spectrometer detector, a fraction collector and a waste liquid recovery device, a sliding ring 3 is movably installed at the top of the detection and collection component 2, a microcapsule 4 is arranged at the bottom of the kettle body 1, an external motor 5 is fixedly installed in the middle of the bottom end of the kettle body 1, the output shaft of the external motor 5 passes through the bottom end of the kettle body 1 and a central rod 6 is fixedly installed, a stirring blade 7 is fixedly installed circumferentially on the central rod 6, a top cover 8 is movably installed in the middle of the top end of the kettle body 1, a feeding pipe 9 is fixedly installed at the top end of the kettle body 1, a partition plate 10 is fixedly installed inside the kettle body 1, and a top middle of the top cover 8 A base 11 is fixedly installed on the top of the base 11, a sample injection assembly 12 is fixedly installed on the top of the base 11, a straight groove 13 is opened on the top cover 8, a vortex guide plate 14 is fixedly installed inside the kettle body 1, an outer sleeve 16 is fixedly installed on the sliding ring 3, a centrifugal separation assembly 15 is movably installed on the outer sleeve 16, an external transmission gear 17 is fixedly installed on the top of the centrifugal separation assembly 15, an external drive assembly 18 is fixedly installed on the side of the base 11, an ultrafiltration separation assembly 19 is fixedly installed on the outside of the kettle body 1, a main ultrasonic transducer 20 is annularly arranged inside the kettle body 1, and the sample injection assembly 12 and the ultrafiltration separation assembly 19, as well as the ultrafiltration separation assembly 19 and the bottom end of the kettle body 1 are connected by a high-pressure pipeline; Compared with the prior art, the present application provides a microcapsule 4 at the bottom end of the kettle body 1, embeds an electromagnetic coil at the bottom of the kettle body 1, and releases the enzymolysis agent in stages by adjusting the magnetic field strength to avoid self-degradation caused by excessive initial concentration. In addition, the present application is combined with a two-layer main ultrasonic transducer 20 provided inside. The main ultrasonic transducer 20 of each layer is evenly distributed in a ring shape, and the frequencies on the upper and lower sides are different. The extraction and separation equipment adopts the synergistic effect of ultrasonic enzymolysis to extract Ganoderma lucidum peptides, and the ultrasonic cavitation effect and the enzymolysis reaction are carried out simultaneously, which significantly improves the Ganoderma lucidum cell wall crushing efficiency and peptide release rate. At the same time, the ultrafiltration separation component 19 adopts a multi-layer gradient ultrafiltration membrane group, and each layer of the membrane group is equipped with an independent temperature control system to achieve ultrafiltration under low temperature conditions, reduce the thermal denaturation of peptides, and realize the preliminary classification of peptide segments by gradient interception, which is convenient for the subsequent fine extraction and separation of peptide segments, and ensures the extraction and separation effect of high equipment. At the same time, by providing a The centrifugal separation component 15 is directly connected to the mass spectrometer through the electrospray ionization interface at the outlet at the bottom of the centrifugal separation component 15, and the mass spectrometer signal is collected in real time. The mass spectrometer data is analyzed by the edge computing module to dynamically adjust the mobile phase ratio and flow rate. In addition, the centrifugal separation component 15 can realize the combination of rotation and revolution through the drive of the external drive component 18 and the drive of the external motor 5 during use, and the centrifugal separation component 15 is a structural setting for adjusting the inclination angle that can be used in conjunction with the external drive component 18 to realize the use of centrifugal force to accelerate the stratification of the two phases, the light phase gathers inwardly, and the heavy phase moves outwardly. In addition, a vortex guide plate 14 is also provided inside to ensure that it realizes efficient vortex field generation and material mixing in the ultrasonic and enzymatic hydrolysis coordinated reactor. At the same time, the vortex guide plate 14 and the inner wall of the reactor body 1 are spaced apart, so that a certain flow channel is formed between the vortex guide plate 14 and the inner wall of the reactor body 1, thereby optimizing the generation of the vortex field and the material mixing effect.

[0020] See also Figure 3-4 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a sliding ring 3, the bottom end of the sliding ring 3 is embedded in the top of the detection and collection component 2 and is sealed and rotated, and the edge coverage of the sliding ring 3 exceeds the top edge of the detection and collection component 2, and a plurality of protruding short rods are arranged on the side of the sliding ring 3, and the short rods are slidably arranged in contact with the bottom surface of the kettle body 1; In this embodiment, it should be noted that the protruding short rod on the edge of the sliding ring 3 can be used to slide and rotate against the bottom end of the inner surface of the kettle body 1 during rotation, so that the internal material will not settle and accumulate, thereby ensuring more sufficient mixing and extraction and separation effects.

[0021] See also Figure 3-4, a device for extracting and separating Ganoderma lucidum peptides for anti-tumor and improving immunity, comprising a microcapsule 4, the microcapsule 4 is designed in a magnetically controlled release mode, the protease is encapsulated in a temperature-sensitive chitosan capsule body, and the surface of the capsule body is coated with Fe3O4 nanoparticles, and an electromagnetic coil is embedded in the bottom end of the inner part of the kettle body 1; In this embodiment, it should be noted that the enzymatic agent in the microcapsule 4 is released in stages by adjusting the magnetic field strength of the electromagnetic coil at the bottom, thereby avoiding self-degradation caused by excessive initial concentration, improving the enzymatic efficiency, and reducing the amount of enzyme used. The residue of the capsule can also be recovered by magnetic separation to ensure high-purity extraction and separation of the Ganoderma lucidum peptide.

[0022] See also Figure 1-3 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a central rod 6, the bottom end of the central rod 6 penetrates the bottom end of the kettle body 1 and is rotatably arranged, and the top end of the central rod 6 is located at the bottom end of the top cover 8 and is fixedly connected, the diameter covered by the stirring blade 7 is one-third of the inner diameter of the kettle body 1, and an inclined blade turbine structure is adopted, the surface of the stirring blade 7 is coated with a polytetrafluoroethylene coating with a thickness of 50-100 μm, and a miniature ultrasonic transducer is embedded inside; In this embodiment, it should be noted that the top cover 8 can be driven to rotate while the center rod 6 rotates, so that the structure connected thereto can be driven to rotate synchronously, thereby increasing the separation effect on the internal material, and the setting of the stirring blade 7 can ensure that it covers most of the area inside the kettle body 1 and reduces the adhesion of the material. At the same time, by periodically utilizing the internally embedded miniature ultrasonic transducer to emit ultrasonic waves, it can further prevent the material from being deposited on the surface of the stirring blade 7, thereby improving the separation and processing effect of the raw materials.

[0023] See also Figure 1-3 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a top cover 8, the top cover 8 is embedded in the top end of the kettle body 1 and is rotatably arranged, the outer side of the top cover 8 is provided with a convex ring and the convex ring is movably clamped in the top end of the kettle body 1, the number of partition plates 10 is multiple and the ring is evenly located inside the kettle body 1 and fixedly installed, the vortex guide plate 14 is spiral and extends upward from the bottom end around the inner wall of the kettle body 1, the width of the vortex guide plate 14 is one tenth of the kettle body 1, and its spiral pitch is one fifth of the height of the kettle body 1, the cross-section of the vortex guide plate 14 adopts an airfoil structure design, and it is made of stainless steel and coated with a polytetrafluoroethylene coating on the surface, and a reinforcing rib is welded on the back thereof, the vortex guide plate 14 is fixed to the inner wall of the kettle body 1 by bolts through an L-shaped bracket and is spaced from the inside of the kettle body 1, and the spacing distance is 10%-20% of the guide plate width, and the partition plate 10 is located in the above-mentioned interval and fixedly installed, so that the vortex guide plate 14 and the inside of the kettle body 1 form multiple flow channels; In this embodiment, it should be noted that the centrifugal separation component 15 installed at the bottom can be driven to rotate during the rotation of the top cover 8, so that the material in the centrifugal separation component 15 can be better separated to ensure the extraction effect. The setting of the vortex guide plate 14 can ensure that the material forms a stable vortex during the rising process. Combined with the rotation of the stirring blade 7 and the rotational shear force opposite to the spiral direction of the vortex guide plate 14, it forms a vortex field together with the vortex guide plate 14, thereby enhancing the effect of material separation and mixing and improving the mixing and extraction efficiency.

[0024] See also Figure 3-6 , a Ganoderma lucidum peptide extraction and separation device for anti-tumor and improving immunity, comprising a centrifugal separation component 15, the centrifugal separation component 15 comprises a connecting straight rod 151, the upper and lower ends of the connecting straight rod 151 are respectively fixedly mounted with external ends 152, a centrifugal spiral tube 153 is fixedly connected between the two external ends 152, a universal joint 154 is fixedly mounted at one end of the external end 152, an external tube 155 is fixedly mounted at the end of the universal joint 154, and an internal hose 156 is provided inside the universal joint 154 to connect the external end 152 and the external tube 155; In this embodiment, it should be noted that a microfluidic chip is arranged in the bottom external pipe 155, and a micro-heating plate is arranged at the bottom, on which a main channel and a branch channel are arranged, and an electrode array is arranged at the intersection of the two channels. The main channel connects the centrifugal spiral tube 153 and the mass spectrometer detector and the waste liquid recovery device in the detection collection component 2. The branch channel is connected to the independent collection pool of the fraction collector. The outer sleeve 16 at the bottom of the external pipe 155 is relatively rotatably arranged, and is fixedly connected to the outer sleeve 16 at the top. An extension ring is arranged on the outside of the external pipe 155, and a plurality of uniformly arranged leakage grooves are opened on the outer sleeve 16. In this way, the target peptide segment in the effluent can be monitored in real time by the mass spectrometer detector, and the voltage can be adjusted in real time by the microfluidic chip, and the target component can be dynamically switched to the designated branch channel, and then collected in the corresponding independent collection pool, while the non-target component continues to flow along the main channel to the waste liquid recovery device. The micro-heating plate can also perform precise temperature control to ensure the stability of the mobile phase temperature.

[0025] See also Figure 4-6 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising an external drive component 18, the external drive component 18 comprises an electric telescopic rod 181, a base rod 182 is fixedly mounted on the end of the electric telescopic rod 181, a stepping motor 183 is fixedly mounted on the bottom end of the base rod 182, and a driving gear 184 is fixedly mounted on the output shaft of the stepping motor 183; In this embodiment, it should be noted that one end of the electric telescopic rod 181 is fixedly mounted on the base 11, a circular hole is opened at the end of the base rod 182, a metal round tube is sealed and rotatably mounted on the top of the external transmission gear 17, and is fixedly connected to the outer sleeve 16, one end of the metal round tube passes through the external transmission gear 17 and is connected to the outer sleeve 16 and the outer end 152, and the other end is connected to the injection assembly 12, the injection assembly 12 includes an injection pump, a mobile phase mixer and an eight-way valve, the base rod 182 is sleeved on the metal round tube, the driving gear 184 is meshed with the external transmission gear 17 and the thickness is less than the thickness of the external transmission gear 17, and the external transmission gear 17 is located in the straight groove 13 and is movably arranged, so that In the process of rotation driven by the top cover 8, the centrifugal separation component 15 as a whole can revolve around the central axis of the equipment, while the stepping motor 183 drives the driving gear 184 to rotate, thereby driving the external transmission gear 17 to rotate. The external transmission gear 17 drives the centrifugal spiral tube 153 to rotate around the axis through the setting of the universal joint 154. In this way, the centrifugal separation component 15 as a whole revolves and rotates at the same time, which greatly improves the separation effect of the material. At the same time, the position of the external transmission gear 17 in the straight groove 13 can be adjusted by the telescopic electric telescopic rod 181, thereby controlling the overall inclination angle of the centrifugal separation component 15, reflecting the requirements for separation and extraction of different peptide segments, and improving the scope of application of the equipment.

[0026] See also Figure 1-3 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising an ultrafiltration separation component 19, wherein the ultrafiltration separation component 19 adopts a three-stage ultrafiltration membrane stacking design in series to form a multi-layer gradient membrane group, the membrane material is polyethersulfone, and each stage of the membrane component is externally connected to a semiconductor refrigeration sheet, and the temperature is adjusted by thermocouple feedback, a high-pressure pulse valve is arranged downstream of the ultrafiltration membrane, and the membrane surface is periodically backwashed, and the surface of the ultrafiltration membrane is etched with bionic leaf vein patterns, and the membrane component interlayer is embedded with a shape memory alloy, and the shape memory alloy automatically adjusts the membrane surface tension according to the temperature change, and one end of the ultrafiltration separation component 19 is connected to the inside of the kettle body 1 through a conduit; In this embodiment, it should be noted that the fluid pressure is dispersed by etching bionic leaf vein patterns using the microfluidic principle, and the embedded shape memory alloy can prevent the membrane structure from deforming, thereby ensuring its service life. In addition, the multi-layer gradient module can better separate and extract, and the large molecular weight peptides that do not meet the separation requirements are returned to the interior of the kettle 1 for secondary enzymatic hydrolysis, thereby ensuring full utilization of the raw materials.

[0027] See also Figure 3 , an anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a main ultrasonic transducer 20, the main ultrasonic transducer 20 is arranged on the upper and lower sides inside the kettle body 1, the upper layer is arranged with a low-frequency transducer, and the lower layer is arranged with a high-frequency transducer to form a superimposed sound field; In this embodiment, it should be noted that the use of superimposed sound fields can improve energy utilization, significantly shorten the time required for enzymatic hydrolysis, and improve the use efficiency of the entire device.

[0028] A method for using a ganoderma lucidum peptide extraction and separation device for anti-tumor and improving immunity comprises the following steps: S1. Equipment startup self-test and automatic loading of process parameters; S2, transporting the raw materials to the interior of the kettle body 1 through the feed pipe 9 for raw material processing; S3, start the main ultrasonic transducer 20 and adjust the parameters, connect the electromagnetic coil at the bottom of the kettle body 1 and adjust the magnetic field strength as required, and start the detection and collection component 2 as required to drive the central rod 6 to rotate, so that the central rod 6 drives the stirring blade 7 and the top cover 8 to rotate, and at the same time adjust the overall inclination angle of the centrifugal separation component 15 through the external drive component 18, and the centrifugal separation component 15 can also be driven to rotate by the external drive component 18 during the rotation process; S4, the raw material after enzymatic hydrolysis is filtered and separated by the ultrafiltration separation component 19, and the peptide segments that do not meet the requirements are returned to the kettle body 1 for secondary enzymatic hydrolysis, and the peptide segments that meet the requirements enter the centrifugal spiral tube 153 through the injection component 12, and then the microfluidic chip at the bottom is used for shunting and collection, and the mass spectrometer detector is monitored in real time and the various parameters of the equipment are dynamically adjusted according to the monitoring results; S5. The collected purified liquid is subjected to subsequent concentration and drying processes to complete the final extraction and separation operation.

[0029] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device, comprising a kettle (1), characterized in that: A detection and collection component (2) is fixedly mounted on the bottom end of the kettle body (1), and the detection and collection component (2) comprises a mass spectrometer detector, a fraction collector and a waste liquid recovery device. A sliding ring (3) is movably mounted on the top end of the detection and collection component (2). A microcapsule (4) is arranged at the bottom end of the kettle body (1). An external motor (5) is fixedly mounted on the middle part of the bottom end of the kettle body (1). The output shaft of the external motor (5) passes through the bottom end of the kettle body (1). A central rod (6) is fixedly mounted on the bottom end of the kettle body (1). A stirring blade (7) is fixedly mounted circumferentially on the central rod (6). A top cover (8) is movably mounted on the middle part of the top end of the kettle body (1). A feed pipe (9) is fixedly mounted on the top end of the kettle body (1). A partition plate (10) is fixedly mounted on the inside of the kettle body (1). A base (11) is fixedly mounted on the middle part of the top end of the top cover (8). A sample introduction component (12) is fixedly mounted on the top of the base (11); a straight groove (13) is formed on the top cover (8); a vortex guide plate (14) is fixedly mounted inside the kettle body (1); an outer sleeve (16) is fixedly mounted on the sliding ring (3); a centrifugal separation component (15) is movably mounted on the outer sleeve (16); an external transmission gear (17) is fixedly mounted on the top of the centrifugal separation component (15); an external drive component (18) is fixedly mounted on the side of the base (11); an ultrafiltration separation component (19) is fixedly mounted on the outside of the kettle body (1); a main ultrasonic transducer (20) is annularly mounted inside the kettle body (1); the sample introduction component (12) and the ultrafiltration separation component (19) as well as the ultrafiltration separation component (19) and the bottom end of the kettle body (1) are connected via a high-pressure pipeline; The centrifugal separation assembly (15) comprises a connecting straight rod (151), the upper and lower ends of the connecting straight rod (151) are respectively fixedly mounted with outer end caps (152), a centrifugal spiral tube (153) is fixedly connected and penetrated between the two outer end caps (152), a universal joint (154) is fixedly mounted on one end of the outer end cap (152), an external tube (155) is fixedly mounted on the end of the universal joint (154), and an inner hose (156) is provided inside the universal joint (154) and penetrates the outer end cap (152) and is connected to the external tube (155); The external drive assembly (18) comprises an electric telescopic rod (181), a base rod (182) is fixedly mounted on the end of the electric telescopic rod (181), a stepping motor (183) is fixedly mounted on the bottom end of the base rod (182), and a driving gear (184) is fixedly mounted on the output shaft of the stepping motor (183).

2. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The bottom end of the sliding ring (3) is embedded in the top end of the detection and collection component (2) and is sealed and rotated, and the edge coverage of the sliding ring (3) exceeds the top edge of the detection and collection component (2). The side of the sliding ring (3) is provided with a plurality of protruding short rods, and the short rods are slidably arranged in contact with the inner bottom surface of the kettle body (1).

3. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The microcapsule (4) is designed in a magnetically controlled release manner, and the protease is encapsulated in a temperature-sensitive chitosan capsule body, and the surface of the capsule body is coated with Fe3O4 nanoparticles. An electromagnetic coil is embedded and installed at the bottom end of the kettle body (1).

4. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The bottom end of the central rod (6) penetrates the bottom end of the kettle body (1) and is rotatably arranged, and the top end of the central rod (6) is located at the bottom end of the top cover (8) and is fixedly connected. The diameter covered by the stirring blade (7) is one third of the inner diameter of the kettle body (1), and a pitch-blade turbine structure is adopted. The surface of the stirring blade (7) is coated with a polytetrafluoroethylene coating with a thickness of 50-100 μm, and a miniature ultrasonic transducer is embedded inside.

5. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The top cover (8) is embedded in the top of the kettle body (1) and is rotatably arranged. The outer side surface of the top cover (8) is provided with a convex ring, and the convex ring is movably clamped on the top of the kettle body (1). The number of the partition plates (10) is multiple and the partition plates (10) are evenly located in an annular shape and fixedly installed inside the kettle body (1). The vortex guide plate (14) is spiral-shaped and extends upward from the bottom end around the inner wall of the kettle body (1). The width of the vortex guide plate (14) is one-tenth of the kettle body (1), and the spiral pitch is one-fifth of the height of the kettle body (1). The cross section of the vortex guide plate (14) is designed with an airfoil structure, and is made of stainless steel and coated with a polytetrafluoroethylene coating on the surface. A reinforcing rib is welded on the back side. The vortex guide plate (14) is fixed to the inner wall of the kettle body (1) by means of an L-shaped bracket using bolts and is spaced apart from the inside of the kettle body (1). The spacing distance is 10%-20% of the width of the guide plate. The partition plate (10) is fixedly installed in the above-mentioned spacing, so that a plurality of flow channels are formed between the vortex guide plate (14) and the inside of the kettle body (1).

6. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The external pipe (155) at the bottom end is provided with a microfluidic chip, and a micro-heating plate is provided at the bottom end. A main channel and a branch channel are provided on the external pipe, and an electrode array is provided at the intersection of the two channels. The main channel connects the centrifugal spiral tube (153) and the mass spectrometer detector and the waste liquid recovery device in the detection collection component (2). The branch channel is connected to the independent collection pool of the fraction collector. The outer sleeve (16) at the bottom end of the external pipe (155) is relatively rotatably arranged, and is fixedly connected to the outer sleeve (16) at the top end. An extension ring is provided on the outside of the external pipe (155), and a plurality of evenly arranged leakage grooves are opened on the outer sleeve (16).

7. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: One end of the electric telescopic rod (181) is fixedly mounted on the base (11); a circular hole is formed at the end of the base rod (182); a metal round tube is sealed and rotatably mounted on the top end of the external transmission gear (17) and is fixedly connected to the outer sleeve (16); one end of the metal round tube passes through the external transmission gear (17) and is connected to the outer sleeve (16) and the outer end head (152); the other end of the metal round tube is connected to the injection assembly (12); the injection assembly (12) includes an injection pump, a mobile phase mixer and an eight-way valve; the base rod (182) is sleeved on the metal round tube; the driving gear (184) meshes with the external transmission gear (17) and has a thickness smaller than that of the external transmission gear (17); the external transmission gear (17) is movably arranged in the straight groove (13).

8. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The ultrafiltration separation component (19) adopts a three-stage ultrafiltration membrane stacking design in series to form a multi-layer gradient membrane group. The membrane material is polyethersulfone, and each stage of the membrane component is externally connected to a semiconductor cooling plate. The temperature is adjusted by thermocouple feedback. A high-pressure pulse valve is arranged downstream of the ultrafiltration membrane to periodically backwash the membrane surface. The ultrafiltration membrane surface is etched with bionic leaf vein patterns. The membrane component interlayer is embedded with a shape memory alloy. The shape memory alloy automatically adjusts the membrane surface tension according to temperature changes. One end of the ultrafiltration separation component (19) is connected to the inside of the kettle body (1) through a conduit.

9. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to claim 1, characterized in that: The main ultrasonic transducer (20) is arranged on the upper and lower sides inside the kettle body (1), with a low-frequency transducer arranged on the upper layer and a high-frequency transducer arranged on the lower layer to form a superimposed sound field.

10. The anti-tumor and immunity-enhancing Ganoderma lucidum peptide extraction and separation device according to any one of claims 1 to 9, characterized in that: The specific usage steps are as follows: S1. Equipment startup self-test and automatic loading of process parameters; S2, transporting the raw materials to the interior of the kettle body (1) through the feed pipe (9) for raw material processing; S3, starting the main ultrasonic transducer (20) and adjusting the parameters, connecting the electromagnetic coil at the bottom of the kettle body (1) and adjusting the magnetic field strength as required, and starting the detection and collection component (2) as required to drive the central rod (6) to rotate, so that the central rod (6) drives the stirring blades (7) and the top cover (8) to rotate, and at the same time adjusting the overall inclination angle of the centrifugal separation component (15) through the external drive component (18), and during the rotation process, the external drive component (18) can also drive the centrifugal separation component (15) to rotate; S4, the raw material after enzymatic hydrolysis is filtered and separated by an ultrafiltration separation component (19), and the peptide segments that do not meet the requirements are returned to the kettle body (1) for secondary enzymatic hydrolysis, and the peptide segments that meet the requirements enter the centrifugal spiral tube (153) through the injection component (12), and then are separated and collected by the microfluidic chip at the bottom, while the mass spectrometer detector monitors in real time and dynamically adjusts various parameters of the equipment according to the monitoring results; S5. The collected purified liquid is subjected to subsequent concentration and drying processes to complete the final extraction and separation operation.

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