Bladder cancer tumor cell organoid culture device and method

Abstract

The invention belongs to the technical field of bladder cancer tumor cell organoid culture, and particularly relates to a bladder cancer tumor cell organoid culture device and method.The bladder cancer tumor cell organoid culture device comprises a culture medium containing mechanism, a liquid discharging mechanism, a lifting driving mechanism, a culture mechanism and a sealing assembly; the culture medium placing mechanism is used for placing a culture medium, the liquid discharging mechanism is rotationally connected to the lower end of the interior of the culture medium placing mechanism, and the outer wall of the liquid discharging mechanism is connected with the driving end of the culture medium placing mechanism. Therefore, the operation steps of changing the liquid are reduced, the difficulty of changing the liquid is reduced, automatic liquid drainage can be realized, and the taken bladder cancer tumor cells are clean and tidy.

Description

Technical Field

[0001] The present invention relates to the technical field of bladder cancer tumor cell organoid culture, and in particular to a bladder cancer tumor cell organoid culture device and method. Background Art

[0002] Bladder cancer tumor cell organoids refer to microstructures formed by bladder cancer tumor cells that have organ-like structure and function.

[0003] In the existing bladder cancer cell organoid culture, the extracted and separated bladder cancer cells need to be cultured in a culture medium to provide the necessary nutrition and environment for the bladder cancer cells. The culture medium needs to be replaced according to the cell growth status and experimental requirements. Generally, it is replaced every three days. If the culture medium turns yellow, it needs to be replaced immediately. When replacing, a direct replacement method can be adopted to adapt the cells to the new culture medium; or a more gentle method can be adopted. When subculturing cells, the new culture medium and the original recommended culture medium are mixed in a certain proportion and gradually replaced, and the cell growth status is closely observed to ensure that the cells can adapt to the new culture medium environment;

[0004] However, the existing devices for culturing bladder cancer tumor cell organoids are relatively simple. Generally, bladder cancer tumor cells and culture medium are directly placed in glass vessels for culture. According to the culture environment required by bladder cancer tumor cells, the vessel needs to be placed in a carbon dioxide incubator for culture. During the culture process, the replacement of the culture medium requires manual operation by the operator. The culture medium to be replaced is gradually taken out through a rubber-tipped dropper or a pipette, and the new culture medium is injected into the glass vessel. After the culture medium is cultivated, the liquid around the bladder cancer tumor cells needs to be removed. Therefore, the culture of bladder cancer tumor cell organoids is not only cumbersome to operate, but also inefficient.

[0005] To this end, we propose a bladder cancer tumor cell organoid culture device and method. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A bladder cancer tumor cell organoid culture device, comprising: a culture medium placement mechanism, a drainage mechanism, a lifting and driving mechanism, a culture mechanism and a sealing component;

[0008] The culture medium placing mechanism is placed on the table, and is used to place the culture medium through the culture medium placing mechanism. The drainage mechanism is rotatably connected to the lower end of the culture medium placing mechanism. The outer wall of the drainage mechanism is connected to the driving end of the culture medium placing mechanism, and is driven by the culture medium placing mechanism to drive the drainage mechanism to rotate so that the drainage mechanism drains the culture medium inside the culture medium placing mechanism. The lifting and lowering driving mechanism is installed at the inner center of the culture medium placing mechanism. The bottom of the lifting and lowering driving mechanism is slidably connected to the inner bottom end of the culture medium placing mechanism, and is used to control the depth of the culture medium through the movement of the lifting and lowering driving mechanism. The culture mechanism is installed on the top of the lifting and lowering driving mechanism, and the bottom center of the culture mechanism contacts the top of the lifting and lowering driving mechanism. At the same time, bladder cancer tumor cells are placed inside the culture mechanism, and is driven by the lifting and lowering driving mechanism to absorb and discharge the culture medium inside the culture mechanism into the inside of the culture mechanism. The sealing component is detachably installed on the top of the culture medium placing mechanism, and is used to seal the culture medium placing mechanism.

[0009] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the culture medium placement mechanism includes: a guide component, a placement component and a drainage drive component;

[0010] The guide component is placed on the desktop, with the top in contact with the bottom of the lifting drive mechanism, the placement component is detachably mounted on the top of the guide component, and the discharge drive component is mounted in the middle of the inner wall of the placement component.

[0011] As a preferred embodiment of the bladder cancer tumor cell organoid culture device of the present invention, the guide assembly comprises: a chassis and an arc groove;

[0012] The chassis is placed on a table, arc grooves are arranged around the surface of the chassis, and the inside of the arc grooves contacts the bottom of the lifting drive mechanism;

[0013] The placement assembly comprises: a placement vessel, an annular partition, a liquid outlet groove, a first installation groove, a placement groove, a rotation groove, a first through groove and a second through groove;

[0014] The placing vessel is detachably mounted on the top of the chassis, the annular partition is arranged in the middle of the inner wall of the placing vessel, the liquid outlet groove is arranged around the bottom of the annular partition, a through hole is arranged at the bottom of the liquid outlet groove, the first mounting groove is arranged in the middle of the inner wall of the annular partition, the placing groove is arranged at the lower end of the inner wall of the placing vessel, the placing groove is arranged at the lower end of the annular partition, the rotating groove is arranged at the lower end of the inner wall of the placing vessel, the rotating groove is arranged at the lower end of the placing groove, the first through groove is arranged at the lower side of the front end of the outer wall of the placing vessel, and the second through groove is arranged in the middle of the left end of the outer wall of the placing vessel;

[0015] The liquid discharge drive assembly includes: a placement box, a rotating motor, and gears;

[0016] The placement box is installed in the middle of the front end of the outer wall of the placement container, the rotating motor is arranged inside the placement box, the gear is connected to the output end of the rotating motor, the rear end of the outer wall of the gear passes through the first through groove, and at the same time, the rear end of the outer wall of the gear is connected to the front end of the outer wall of the discharge mechanism.

[0017] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the drainage mechanism comprises: a blocking component and a drainage component;

[0018] The blocking component is rotatably connected inside the placement groove, the top of the blocking component contacts the bottom of the annular partition, the drainage component is fixedly installed inside the placement groove, and the top of the drainage component is rotatably connected to the bottom of the blocking component.

[0019] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the blocking component includes: a first rotating disk, a second mounting groove, a tooth groove, a return spring, a second rotating disk and a blocking plate;

[0020] The first rotating disk is rotatably connected to the inside of the placement groove, the second installation groove is arranged on the outside of the surface of the first rotating disk, the tooth groove is arranged around the outer wall of the first rotating disk, the tooth groove is meshed with the gear, the return spring is arranged around the inside of the second installation groove, the second rotating disk is slidably connected to the inside of the second installation groove, the bottom of the second rotating disk is connected to the top of the return spring, and at the same time, the top of the second rotating disk contacts the bottom of the annular partition, the blocking plate is arranged around the top of the second rotating disk, and the top of the blocking plate contacts the inside of the liquid outlet groove;

[0021] The drainage assembly comprises: a drainage tray and a drainage pipe;

[0022] The drain pan is fixedly installed at the lower end of the placement slot, the top of the drain pan is rotatably connected to the bottom of the first rotating disc, the drain pipe is arranged at the left end of the outer wall of the drain pan, and the drain pipe runs through the second through slot.

[0023] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the lifting and driving mechanism includes: a limit component and a lifting and driving component;

[0024] The limit assembly is rotatably connected inside the rotating groove, the outer wall of the lifting drive assembly is installed inside the first installation groove, the lower end of the lifting drive assembly is rotatably connected around the inside of the limit assembly, and the bottom of the lifting drive assembly is slidably connected inside the arc groove.

[0025] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the limiting assembly includes: a limiting plate, a third mounting slot and a limiting rod;

[0026] A limiting rod is arranged around the outer wall of the limiting plate, and an outer end of the limiting rod is slidably connected to the inside of the rotating groove, and a third mounting groove is arranged around the surface of the limiting plate;

[0027] The lifting drive assembly comprises: a rubber sheet, a mounting plate, a hydraulic cylinder, a sealing box, a grab bar and a knob block;

[0028] The outer wall of the rubber sheet is installed inside the first mounting groove, the mounting plate is set at the center of the rubber sheet, and at the same time, the inner end of the rubber sheet is installed around the outer wall of the mounting plate, the hydraulic cylinder is installed at the top center of the mounting plate, and the sealing box is installed on the top of the mounting plate. At the same time, the sealing box seals and wraps the hydraulic cylinder, the top of the grab bar is rotatably connected around the output end of the hydraulic cylinder, the middle two ends of the grab bar are rotatably connected to the inside of the third mounting groove, the bottom of the grab bar is slidably connected to the inside of the arc groove, the knob block is set around the top of the sealing box, and the knob block is connected to the bottom of the culture mechanism.

[0029] As a preferred embodiment of the bladder cancer tumor cell organoid culture device of the present invention, the culture mechanism comprises: a culture component and an auxiliary component;

[0030] The bottom of the culture component is detachably mounted on the top of the sealed box, and the bottom of the culture component is in contact with the top of the annular partition. At the same time, bladder cancer tumor cells are placed inside the culture component, and the auxiliary component is detachably mounted on the top of the culture component.

[0031] The culture assembly comprises: a culture vessel, a liquid transport pipeline, a slide groove, a piston and a knob groove;

[0032] The culture vessel is placed inside the placement vessel, bladder cancer tumor cells are placed inside the culture vessel, a liquid transport pipeline is arranged around the upper end of the outer wall of the culture vessel, the liquid transport pipeline and the culture vessel are interconnected, the bottom of the liquid transport pipeline contacts the surface of the annular partition, a slide groove is arranged on the inner wall of the culture vessel, a piston is slidably connected inside the slide groove, a knob groove is arranged around the bottom of the piston, and a knob block is detachably installed inside the knob groove;

[0033] The auxiliary components include: an auxiliary cover plate and a telescopic filter plate;

[0034] The auxiliary cover is detachably mounted on the top of the culture vessel, the top of the auxiliary cover contacts the bottom of the sealing assembly, the telescopic filter plate is arranged at the bottom center of the auxiliary cover, and the bottom of the telescopic filter plate contacts the surface of the piston.

[0035] As a preferred embodiment of the bladder cancer tumor cell organoid culture device described in the present invention, the sealing component includes: a sealing cover plate, an observation port and an injection valve;

[0036] The sealing cover plate is detachably mounted on the top of the container, the observation port is arranged at the front end of the surface of the sealing cover plate, and the liquid injection valve is arranged at the right end of the surface of the sealing cover plate.

[0037] A method for using a bladder cancer tumor cell organoid culture device, comprising the following operation methods:

[0038] S1: The culture vessel is placed on the top of the sealed box. At this time, the knob groove at the bottom of the piston is inserted into the knob block. By rotating the culture vessel, the piston is driven to rotate, so that one end of the knob block is inserted into the groove on the side of the inner wall of the knob groove, thereby limiting the placement of the culture vessel;

[0039] S2: The extracted and separated bladder cancer tumor cells are placed in the culture vessel and received by the surface of the piston, and then the auxiliary cover is pressed and installed on the top of the culture vessel. At this time, the bottom of the telescopic filter plate contacts the surface of the piston, so that the telescopic filter plate wraps and isolates the bladder cancer tumor cells, and the sealing cover is connected to the placement vessel by pressing to seal the placement vessel;

[0040] S3: When culturing, the culture medium with a configured concentration is injected into the interior of the placement vessel through the injection valve for storage. At this time, the blocking plate of the second rotating disk blocks the liquid outlet groove of the annular partition to prevent the culture medium from being discharged. By starting the hydraulic cylinder, the hydraulic cylinder drives the grab bar to expand and contract, and under the arc groove limit on the bottom plate surface, the rubber sheet and the mounting plate are forced to rotate and lift at the same time. Since the top of the auxiliary cover plate contacts the bottom of the sealing cover plate, the movement of the sealing box forces the piston to perform a rotational lifting movement, so that the culture medium inside the placement vessel enters the interior of the culture vessel through the liquid transport pipe, infiltrates the bladder cancer tumor cells, and thus cultivates the bladder cancer tumor cells;

[0041] S4: When the culture medium needs to be replaced after a period of time or when the culture medium is observed through the observation port, the rotating motor is started to drive the gear and the first rotating disk to rotate, thereby rotating the second rotating disk. Under the action of the reset spring, when the blocking plate rotates and disengages from the liquid outlet tank, the second rotating disk sinks, so that the blocking plate smoothly disengages from the liquid outlet tank. At this time, the excess culture medium inside the placement vessel falls into the inside of the drainage plate through the first rotating disk and the second rotating disk, and is discharged through the drainage pipe. After being drained clean, the blocking plate and the liquid outlet tank are restored to their initial positions, and the culture medium to be replaced is injected into the placement vessel through the injection valve. At this time, the hydraulic cylinder is started again to allow the new culture medium to interact and mix with the culture medium inside the culture vessel.

[0042] S5: After the culture is completed, the culture fluid is drained cleanly by driving the hydraulic cylinder, and then the culture vessel is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel.

[0043] Compared with existing technologies:

[0044] The present invention stores bladder cancer tumor cells separately by setting a culture mechanism, thereby preventing the bladder cancer tumor cells from spreading to the entire vessel due to tilted placement during culture, thereby increasing the difficulty of liquid replacement operation;

[0045] The present invention enables the new culture medium to interact and fuse with the culture medium inside the culture mechanism through the mutual cooperation of the drainage mechanism, the lifting drive mechanism and the culture mechanism, thereby reducing the operation steps of changing the liquid, reducing the difficulty of changing the liquid, and realizing autonomous drainage, so that the removed bladder cancer tumor cells are cleaner and neater. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 A schematic diagram of the overall main structure provided by the present invention;

[0047] Figure 2 A schematic diagram of the overall main view split structure provided by the present invention;

[0048] Figure 3 A schematic diagram of the internal structure of the culture medium placement mechanism provided by the present invention;

[0049] Figure 4 A schematic diagram of the split structure of the culture medium placement mechanism provided by the present invention;

[0050] Figure 5 A schematic diagram of the structure of the guide assembly provided by the present invention;

[0051] Figure 6 A bottom-up structural schematic diagram of a placement assembly provided by the present invention;

[0052] Figure 7 A schematic diagram of the disassembled structure of the liquid discharge drive assembly provided by the present invention;

[0053] Figure 8 A schematic diagram of the disassembled structure of the liquid discharge mechanism provided by the present invention;

[0054] Fig. 9 A schematic diagram of the structure of the second installation slot provided by the present invention;

[0055] Fig.10 A schematic diagram of the connection structure of the lifting drive mechanism provided by the present invention;

[0056] Fig.11 A schematic diagram of the disassembled structure of the lifting drive mechanism provided by the present invention;

[0057] Fig.12 A schematic diagram of the structure of the lifting drive assembly provided by the present invention;

[0058] Fig.13 A schematic diagram of the disassembly structure of the grab bar and the limit assembly provided by the present invention;

[0059] Fig.14 This is a schematic diagram of the split structure of the culture mechanism provided by the present invention.

[0060] In the figure:

[0061] Culture medium placement mechanism 1, guide component 11, chassis 111, arc groove 112, placement component 12, placement vessel 121, annular partition 122, liquid outlet groove 123, first mounting groove 124, placement groove 125, rotation groove 126, first through groove 127, second through groove 128, drainage drive component 13, placement box 131, rotating motor 132, gear 133, drainage mechanism 2, blocking component 21, first rotating disk 211, second mounting groove 212, tooth groove 213, reset spring 214, second rotating disk 215, blocking plate 216, drainage component 22, drainage Disk 221, drainage pipe 222, lifting drive mechanism 3, limit assembly 31, limit disk 311, third mounting groove 312, limit rod 313, lifting drive assembly 32, rubber sheet 321, mounting plate 322, limit seat 323, hydraulic cylinder 324, sealing box 325, grab rod 326, culture mechanism 4, culture assembly 41, culture vessel 411, liquid transport pipe 413, mounting seat 414, slide 415, piston 416, auxiliary assembly 42, auxiliary cover plate 421, telescopic filter plate 422, sealing assembly 5, sealing cover plate 51, observation port 52, injection valve 53. DETAILED DESCRIPTION

[0062] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0063] The present invention provides a bladder cancer tumor cell organoid culture device and method, please refer to Figure 1-Figure 14 , including a culture medium placing mechanism 1, a liquid discharging mechanism 2, a lifting and driving mechanism 3, a culture mechanism 4 and a sealing component 5;

[0064] The culture medium placement mechanism 1 is placed on the desktop, and the culture medium placement mechanism 1 is used to place the culture medium. The culture medium placement mechanism 1 includes: a guide component 11, a chassis 111, an arc groove 112, a placement component 12, a placement vessel 121, an annular partition 122, a liquid outlet groove 123, a first installation groove 124, a placement groove 125, a rotation groove 126, a first through groove 127, a second through groove 128, a liquid discharge drive component 13, a placement box 131, a rotating motor 132 and a gear 133. The guide component 11 is placed on the desktop, and the top is in contact with the bottom of the lifting drive mechanism 3. The guide component 11 can limit and guide the drive of the lifting drive mechanism 3. The chassis 111 is placed on The desktop comprises an arc groove 112 arranged around the surface of the chassis 111, the interior of the arc groove 112 contacts the bottom of the lifting drive mechanism 3, and the arc groove 112 can limit and guide the driving of the lifting drive mechanism 3, the placement component 12 can be detachably installed on the top of the guide component 11, and the discharge mechanism 2 and the lifting drive mechanism 3 can be installed and placed through the placement component 12, the placement vessel 121 can be detachably installed on the top of the chassis 111, the annular partition 122 is arranged in the middle of the inner wall of the placement vessel 121, and the culture medium can be stored through the annular partition 122, the liquid outlet trough 123 is arranged around the bottom of the annular partition 122, and the bottom of the liquid outlet trough 123 is provided with a through hole, and The stored culture medium can be discharged through the liquid outlet groove 123, the first installation groove 124 is arranged in the middle of the inner wall of the annular partition 122, and the lifting drive mechanism 3 can be installed and placed through the first installation groove 124, the placement groove 125 is arranged at the lower end of the inner wall of the placement vessel 121, the placement groove 125 is arranged at the lower end of the annular partition 122, and the liquid discharge mechanism 2 can be limited and guided through the placement groove 125, the rotation groove 126 is arranged at the lower end of the inner wall of the placement vessel 121, the rotation groove 126 is arranged at the lower end of the placement groove 125, and the lower end component of the lifting drive mechanism 3 can be installed and limited through the rotation groove 126, and the first through groove 127 is arranged at the placement vessel 12 1, the second through groove 128 is arranged in the middle of the left end of the outer wall of the placing vessel 121, the discharge drive component 13 is installed in the middle of the inner wall of the placing component 12, and the culture medium can be placed through the cooperation of the placing component 12 and the discharge drive component 13, the placing box 131 is installed in the middle of the front end of the outer wall of the placing vessel 121, the rotating motor 132 is arranged inside the placing box 131, the gear 133 is connected to the output end of the rotating motor 132, the outer wall rear end of the gear 133 passes through the first through groove 127, and at the same time, the outer wall rear end of the gear 133 is connected to the outer wall front end of the discharge mechanism 2, and the rotation of the gear 133 can drive the discharge mechanism 2 to rotate.

[0065] The drainage mechanism 2 is rotatably connected to the lower end of the culture medium placement mechanism 1, and the outer wall of the drainage mechanism 2 is connected to the driving end of the culture medium placement mechanism 1, and is used to drive the drainage mechanism 2 to rotate through the driving of the culture medium placement mechanism 1, so that the drainage mechanism 2 drains the culture medium inside the culture medium placement mechanism 1. The drainage mechanism 2 includes: a blocking component 21, a first rotating disk 211, a second mounting groove 212, a tooth groove 213, a reset spring 214, a second rotating disk 215, a blocking plate 216, a drainage component 22, a drainage disk 221 and a drainage pipeline 222, the blocking component 21 is rotatably connected to the inside of the placement groove 125, the top of the blocking component 21 contacts the bottom of the annular partition 122, and the blocking component 21 can block the drainage of the annular partition 122, the first rotating disk 211 is rotatably connected to the inside of the placement groove 125, the second installation groove 212 is arranged on the outside of the surface of the first rotating disk 211, the tooth groove 213 is arranged around the outer wall of the first rotating disk 211, the tooth groove 213 is meshed and connected with the gear 133, and the return spring 214 is arranged around the inside of the second installation groove 212 The second rotating disk 215 is slidably connected to the inside of the second mounting groove 212, and the bottom of the second rotating disk 215 is connected to the top of the return spring 214. At the same time, the top of the second rotating disk 215 contacts the bottom of the annular partition 122. The blocking plate 216 is arranged around the top of the second rotating disk 215. The top of the blocking plate 216 contacts the inside of the liquid outlet groove 123, and the blocking plate 216 can block the culture medium discharged from the liquid outlet groove 123. The drainage component 22 is fixedly installed in the placement groove 125, and the top of the drainage component 22 contacts the bottom of the annular partition 122. The bottom of the blocking component 21 is rotatably connected, and the collected culture medium can be discharged through the drainage component 22. The drainage plate 221 is fixedly installed at the inner lower end of the placement groove 125. The top of the drainage plate 221 is rotatably connected to the bottom of the first rotating plate 211, and the culture medium discharged from the outlet groove 123 can be collected through the drainage plate 221. The drainage pipe 222 is arranged at the left end of the outer wall of the drainage plate 221. The drainage pipe 222 runs through the second through groove 128, and the culture medium collected by the drainage plate 221 can be discharged through the drainage pipe 222.

[0066] The lifting drive mechanism 3 is installed in the inner center of the culture medium placing mechanism 1, and the bottom of the lifting drive mechanism 3 is slidably connected to the inner bottom end of the culture medium placing mechanism 1, and the activity of the lifting drive mechanism 3 is used to control the depth of the culture medium. The lifting drive mechanism 3 includes: a limit assembly 31, a limit plate 311, a third mounting groove 312, a limit rod 313, a lifting drive assembly 32, a rubber sheet 321, a mounting plate 322, a hydraulic cylinder 324, a sealing box 325, a grab rod 326 and a knob block 327. The limit assembly 31 is rotatably connected to the inside of the rotating groove 126, and the outer wall of the lifting drive assembly 32 is installed in the inside of the first mounting groove 124. The limit rod 313 is arranged around the outer wall of the limit plate 311, and the outer port of the limit rod 313 is slidably connected to the inside of the rotating groove 126. The third mounting groove 312 is arranged around the surface of the limit plate 311. The lower end of the lifting drive assembly 32 is rotatably connected to the inner periphery of the limit assembly 31, and the bottom of the lifting drive assembly 32 is slidably connected to the inside of the arc groove 112. The rubber sheet The outer wall of 321 is installed inside the first installation groove 124, the installation plate 322 is set at the center of the rubber sheet 321, and the inner end of the rubber sheet 321 is installed around the outer wall of the installation plate 322. The hydraulic cylinder 324 is installed at the top center of the installation plate 322, and the sealing box 325 is installed on the top of the installation plate 322. At the same time, the sealing box 325 seals and wraps the hydraulic cylinder 324. The top of the grab bar 326 is rotatably connected to the output end of the hydraulic cylinder 324. The middle ends of the grab bar 326 are rotatable. Connected to the inside of the third mounting groove 312, the bottom of the grab rod 326 is slidably connected to the inside of the arc groove 112, and the grab rod 326 can be driven to expand and contract by the drive of the hydraulic cylinder 324, so that the grab rod 326 can drive the rubber sheet 321 and the mounting plate 322 to rotate and lift, and the knob block 327 is arranged around the top of the sealing box 325. The knob block 327 is connected to the bottom of the culture mechanism 4, and the components at the bottom of the culture mechanism 4 can be driven by the knob block 327.

[0067] The culture mechanism 4 is installed on the top of the lifting drive mechanism 3, and the bottom center of the culture mechanism 4 is in contact with the top of the lifting drive mechanism 3. At the same time, bladder cancer tumor cells are placed inside the culture mechanism 4, and the culture medium inside the culture medium placement mechanism 1 is sucked and discharged into the culture mechanism 4 through the driving of the lifting drive mechanism 3. The culture mechanism 4 includes: a culture component 41, a culture vessel 411, a liquid transport pipeline 412, a slide 413, a piston 414, a knob groove 415, an auxiliary component 42, an auxiliary cover plate 421 and a telescopic filter plate 422. The culture component 41 The bottom can be detachably mounted on the top of the sealed box 325. The bottom of the culture component 41 is in contact with the top of the annular partition 122. At the same time, bladder cancer tumor cells are placed inside the culture component 41, and the internal components of the culture component 41 can be driven by the hydraulic cylinder 324 to move up and down. The culture vessel 411 is placed inside the placement vessel 121, and bladder cancer tumor cells are placed inside the culture vessel 411. The liquid transport pipe 412 is arranged around the upper end of the outer wall of the culture vessel 411, and the liquid transport pipe 412 is connected to the culture vessel 411. The liquid transport pipe 412 is connected to the culture vessel 411. The bottom of the pipe 412 contacts the surface of the annular partition 122, the slide groove 413 is arranged on the inner wall of the culture vessel 411, the piston 414 is slidably connected to the inside of the slide groove 413, and the bladder cancer tumor cells can be pushed by the piston 414, the knob groove 415 is arranged around the bottom of the piston 414, the knob block 327 can be detachably installed inside the knob groove 415, and the placement of the culture vessel 411 can be limited by the cooperation of the knob groove 415 and the knob block 327. The auxiliary component 42 can be detachably installed on the top of the culture component 41, and through the auxiliary component 42 can seal the culture component 41. At the same time, the auxiliary component 42 can wrap and isolate the placed bladder cancer tumor cells. The auxiliary cover 421 can be detachably installed on the top of the culture vessel 411. The top of the auxiliary cover 421 contacts the bottom of the sealing component 5. The telescopic filter plate 422 is arranged at the bottom center of the auxiliary cover 421. The bottom of the telescopic filter plate 422 contacts the surface of the piston 414. The placed bladder cancer tumor cells can be wrapped and isolated by the telescopic filter plate 422. At the same time, the telescopic filter plate 422 can be extended and retracted as the piston 414 rises and falls.

[0068] The sealing component 5 can be detachably mounted on the top of the culture medium placing mechanism 1, and is used to seal the culture medium placing mechanism 1 through the sealing component 5. The sealing component 5 includes: a sealing cover plate 51, an observation port 52 and an injection valve 53. The sealing cover plate 51 can be detachably mounted on the top of the placing vessel 121, and the placing vessel 121 can be sealed and packaged through the sealing cover plate 51. The observation port 52 is arranged at the front end of the surface of the sealing cover plate 51, and the internal situation of the placing vessel 121 can be observed through the observation port 52. The injection valve 53 is arranged at the right end of the surface of the sealing cover plate 51, and the culture medium can be injected into the interior of the placing vessel 121 through the injection valve 53.

[0069] During specific use, a person skilled in the art places the culture vessel 411 on the top of the sealed box 325. At this time, the knob groove 415 at the bottom of the piston 414 is inserted by the knob block 327. By rotating the culture vessel 411, the piston 414 is driven to rotate, so that one end of the knob block 327 is inserted into the groove on the side of the inner wall of the knob groove 415, thereby limiting the placement of the culture vessel 411. By placing the extracted and separated bladder cancer tumor cells into the culture vessel 411 and receiving them on the surface of the piston 414, the auxiliary cover plate 421 is pressed and installed on the top of the culture vessel 411. At this time, the bottom of the telescopic filter plate 422 contacts the surface of the piston 414, so that the telescopic filter plate 422 wraps and isolates the bladder cancer tumor cells, and passes The sealing cover plate 51 is connected to the placing vessel 121 by pressing, and the placing vessel 121 is sealed. When culturing, the culture medium of the configured concentration is injected into the placing vessel 121 through the injection valve 53 for storage. At this time, the blocking plate 216 of the second rotating disk 215 blocks the liquid outlet groove 123 of the annular partition 122 to prevent the culture medium from draining. By starting the hydraulic cylinder 324, the hydraulic cylinder 324 drives the grab bar 326 to expand and contract, and under the limit of the arc groove 112 on the surface of the bottom plate 111, the rubber sheet 321 and the mounting plate 322 are forced to rotate and rise and fall at the same time. Since the top of the auxiliary cover plate 421 contacts the bottom of the sealing cover plate 51, the activity of the sealing box 325 The piston 414 is forced to perform a rotational lifting movement, so that the culture medium inside the placement vessel 121 enters the interior of the culture vessel 411 through the liquid transport pipe 412 to penetrate the bladder cancer tumor cells, thereby culturing the bladder cancer tumor cells. At the same time, the injection valve 53 can be externally connected to carbon dioxide or other gases required for culture, so that the bladder cancer tumor cells are continuously cultured inside the culture vessel 411. After a period of time or when the culture medium is observed through the observation port 52 and it is necessary to replace the culture medium, the rotating motor 132 is started to drive the gear 133 and the first rotating disk 211 to rotate, thereby rotating the second rotating disk 215. Under the action of the reset spring 214, the blocking plate 216 rotates. When the second rotating disk 215 is separated from the liquid outlet trough 123, the second rotating disk 215 sinks, so that the blocking plate 216 is smoothly separated from the liquid outlet trough 123. At this time, the excess culture medium in the placement vessel 121 falls into the interior of the drainage plate 221 through the first rotating disk 211 and the second rotating disk 215, and is discharged through the drainage pipe 222. After being drained clean, the blocking plate 216 and the liquid outlet trough 123 are restored to their initial positions, and the culture medium to be replaced is injected into the placement vessel 121 through the injection valve 53. At this time, the hydraulic cylinder 324 is started again to allow the new culture medium to interact and mix with the culture medium in the culture vessel 411. The bladder cancer tumor cells are always in the interior of the telescopic filter plate 422 under the isolation of the telescopic filter plate 422. After the culture is completed,The culture fluid is drained cleanly by driving the hydraulic cylinder 324, and then the culture vessel 411 is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel 411.

[0070] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A bladder cancer tumor cell organoid culture device, characterized by: include: A culture medium placement mechanism (1), a liquid discharge mechanism (2), a lifting and driving mechanism (3), a culture mechanism (4) and a sealing component (5); The culture medium placement mechanism (1) is placed on a table, and is used to place the culture medium through the culture medium placement mechanism (1); the drainage mechanism (2) is rotatably connected to the lower end of the culture medium placement mechanism (1); the outer wall of the drainage mechanism (2) is connected to the driving end of the culture medium placement mechanism (1), and is driven by the culture medium placement mechanism (1) to drive the drainage mechanism (2) to rotate, so that the drainage mechanism (2) drains the culture medium inside the culture medium placement mechanism (1); the lifting drive mechanism (3) is installed at the inner center of the culture medium placement mechanism (1), and the bottom of the lifting drive mechanism (3) is slidably connected to the inner side of the culture medium placement mechanism (1). The culture medium placing mechanism (1) is provided at the bottom end of the culture medium placing mechanism (1), and the movement of the lifting drive mechanism (3) is used to control the depth of the culture medium. The culture mechanism (4) is installed at the top of the lifting drive mechanism (3), and the bottom center of the culture mechanism (4) is in contact with the top of the lifting drive mechanism (3). At the same time, bladder cancer tumor cells are placed inside the culture mechanism (4), and the lifting drive mechanism (3) is driven to absorb and discharge the culture medium inside the culture medium placing mechanism (1) into the culture mechanism (4). The sealing component (5) is detachably installed on the top of the culture medium placing mechanism (1), and the sealing component (5) is used to seal the culture medium placing mechanism (1).

2. The bladder cancer tumor cell organoid culture device according to claim 1, characterized in that: The culture medium placement mechanism (1) comprises: a guide component (11), a placement component (12) and a liquid discharge drive component (13); The guide component (11) is placed on a desktop, with its top in contact with the bottom of the lifting drive mechanism (3); the placement component (12) is detachably mounted on the top of the guide component (11); and the discharge drive component (13) is mounted in the middle of the inner wall of the placement component (12).

3. The bladder cancer tumor cell organoid culture device according to claim 2, characterized in that: The guide assembly (11) comprises: a chassis (111) and an arc groove (112); The bottom plate (111) is placed on a table, the arc groove (112) is arranged around the surface of the bottom plate (111), and the inside of the arc groove (112) contacts the bottom of the lifting drive mechanism (3); The placement component (12) comprises: a placement vessel (121), an annular partition (122), a liquid outlet groove (123), a first installation groove (124), a placement groove (125), a rotation groove (126), a first through groove (127) and a second through groove (128); The placing vessel (121) is detachably mounted on the top of the chassis (111); the annular partition (122) is arranged in the middle of the inner wall of the placing vessel (121); the liquid outlet groove (123) is arranged around the bottom of the annular partition (122); a through hole is arranged at the bottom of the liquid outlet groove (123); the first mounting groove (124) is arranged in the middle of the inner wall of the annular partition (122); the placing groove (125) is arranged at the lower end of the inner wall of the placing vessel (121); the placing groove (125) is arranged at the lower end of the annular partition (122); the rotating groove (126) is arranged at the lower end of the inner wall of the placing vessel (121); the rotating groove (126) is arranged at the lower end of the placing groove (125); the first through groove (127) is arranged at the lower side of the front end of the outer wall of the placing vessel (121); and the second through groove (128) is arranged in the middle of the left end of the outer wall of the placing vessel (121); The liquid discharge drive assembly (13) comprises: a placement box (131), a rotating motor (132), and a gear (133); The placement box (131) is installed in the middle of the front end of the outer wall of the placement container (121), the rotating motor (132) is arranged inside the placement box (131), the gear (133) is connected to the output end of the rotating motor (132), the rear end of the outer wall of the gear (133) passes through the first through groove (127), and at the same time, the rear end of the outer wall of the gear (133) is connected to the front end of the outer wall of the liquid discharge mechanism (2).

4. The bladder cancer tumor cell organoid culture device according to claim 3, characterized in that: The liquid discharge mechanism (2) comprises: a blocking component (21) and a liquid discharge component (22); The blocking component (21) is rotatably connected to the inside of the placement groove (125), the top of the blocking component (21) contacts the bottom of the annular partition (122), the drainage component (22) is fixedly installed in the placement groove (125), and the top of the drainage component (22) is rotatably connected to the bottom of the blocking component (21).

5. The bladder cancer tumor cell organoid culture device according to claim 4, characterized in that: The blocking assembly (21) comprises: a first rotating disk (211), a second mounting groove (212), a tooth groove (213), a return spring (214), a second rotating disk (215) and a blocking plate (216); The first rotating disk (211) is rotatably connected to the inside of the placement groove (125), the second installation groove (212) is arranged on the outside of the surface of the first rotating disk (211), the tooth groove (213) is arranged around the outer wall of the first rotating disk (211), the tooth groove (213) is meshed and connected with the gear (133), the return spring (214) is arranged around the inside of the second installation groove (212), the second rotating disk (215) is slidably connected to the inside of the second installation groove (212), the bottom of the second rotating disk (215) is connected to the top of the return spring (214), and at the same time, the top of the second rotating disk (215) contacts the bottom of the annular partition (122), the blocking plate (216) is arranged around the top of the second rotating disk (215), and the top of the blocking plate (216) contacts the inside of the liquid outlet groove (123); The drainage assembly (22) comprises: a drainage tray (221) and a drainage pipe (222); The drain pan (221) is fixedly mounted at the lower end of the placement groove (125); the top of the drain pan (221) is rotatably connected to the bottom of the first rotating disc (211); the drain pipe (222) is disposed at the left end of the outer wall of the drain pan (221); and the drain pipe (222) runs through the second through groove (128).

6. The bladder cancer tumor cell organoid culture device according to claim 5, characterized in that: The lifting drive mechanism (3) comprises: a limit assembly (31) and a lifting drive assembly (32); The limiting assembly (31) is rotatably connected to the inside of the rotating groove (126), the outer wall of the lifting drive assembly (32) is installed inside the first installation groove (124), the lower end of the lifting drive assembly (32) is rotatably connected to the inside of the limiting assembly (31), and the bottom of the lifting drive assembly (32) is slidably connected to the inside of the arc groove (112).

7. The bladder cancer tumor cell organoid culture device according to claim 6, characterized in that: The limiting assembly (31) comprises: a limiting plate (311), a third installation slot (312) and a limiting rod (313); A limiting rod (313) is provided around the outer wall of the limiting plate (311), and the outer end of the limiting rod (313) is slidably connected to the inside of the rotating groove (126), and the third installation groove (312) is provided around the surface of the limiting plate (311); The lifting drive assembly (32) comprises: a rubber sheet (321), a mounting plate (322), a hydraulic cylinder (324), a sealing box (325), a grab bar (326) and a knob block (327); The outer wall of the rubber sheet (321) is installed inside the first installation groove (124), the installation plate (322) is set at the center position of the rubber sheet (321), and at the same time, the inner side end of the rubber sheet (321) is installed around the outer wall of the installation plate (322), the hydraulic cylinder (324) is installed at the top center of the installation plate (322), and the sealing box (325) is installed on the top of the installation plate (322). At the same time, the sealing box (325) seals and wraps the hydraulic cylinder (324), the top of the grab rod (326) is rotatably connected to the output end of the hydraulic cylinder (324), the middle two ends of the grab rod (326) are rotatably connected to the inside of the third installation groove (312), and the bottom of the grab rod (326) is slidably connected to the inside of the arc groove (112), and the knob block (327) is set around the top of the sealing box (325), and the knob block (327) is connected to the bottom of the culture mechanism (4).

8. The bladder cancer cell organoid culture device according to claim 7, characterized in that: The culture mechanism (4) comprises: a culture component (41) and an auxiliary component (42); The bottom of the culture component (41) is detachably mounted on the top of the sealed box (325), and the bottom of the culture component (41) is in contact with the top of the annular partition (122) on all sides. At the same time, bladder cancer tumor cells are placed inside the culture component (41), and the auxiliary component (42) is detachably mounted on the top of the culture component (41); The culture assembly (41) comprises: a culture vessel (411), a liquid transport pipeline (412), a slide groove (413), a piston (414) and a knob groove (415); The culture vessel (411) is placed inside the placement vessel (121), and bladder cancer tumor cells are placed inside the culture vessel (411). The liquid transport pipe (412) is arranged around the upper end of the outer wall of the culture vessel (411), the liquid transport pipe (412) and the culture vessel (411) are interconnected, and the bottom of the liquid transport pipe (412) contacts the surface of the annular partition (122). The slide groove (413) is arranged on the inner wall of the culture vessel (411), and the piston (414) is slidably connected to the inside of the slide groove (413). The knob groove (415) is arranged around the bottom of the piston (414), and the knob block (327) is detachably installed inside the knob groove (415); The auxiliary component (42) comprises: an auxiliary cover plate (421) and a telescopic filter plate (422); The auxiliary cover plate (421) is detachably mounted on the top of the culture vessel (411), the top of the auxiliary cover plate (421) contacts the bottom of the sealing assembly (5), the telescopic filter plate (422) is arranged at the bottom center of the auxiliary cover plate (421), and the bottom of the telescopic filter plate (422) contacts the surface of the piston (414).

9. The bladder cancer tumor cell organoid culture device according to claim 8, characterized in that: The sealing assembly (5) comprises: a sealing cover plate (51), an observation port (52) and a liquid injection valve (53); The sealing cover plate (51) is detachably mounted on the top of the placing vessel (121), the observation port (52) is arranged at the front end of the surface of the sealing cover plate (51), and the injection valve (53) is arranged at the right end of the surface of the sealing cover plate (51).

10. A culture method using the bladder cancer tumor cell organoid culture device according to any one of claims 1 to 9, characterized in that: The following methods are included: S1: The culture vessel (411) is placed on the top of the sealed box (325), and the knob groove (415) at the bottom of the piston (414) is inserted by the knob block (327). The culture vessel (411) is rotated to drive the piston (414) to rotate, so that one end of the knob block (327) is inserted into the groove on the side of the inner wall of the knob groove (415), thereby limiting the placement of the culture vessel (411); S2: The extracted and separated bladder cancer tumor cells are placed in the culture vessel (411) and received by the surface of the piston (414), and then the auxiliary cover plate (421) is pressed and installed on the top of the culture vessel (411). At this time, the bottom of the telescopic filter plate (422) contacts the surface of the piston (414), so that the telescopic filter plate (422) wraps and isolates the bladder cancer tumor cells, and the sealing cover plate (51) is connected to the placement vessel (121) by pressing, so that the placement vessel (121) is sealed; S3: When culturing, the culture medium of the configured concentration is injected into the interior of the placement vessel (121) through the injection valve (53) for storage. At this time, the blocking plate (216) of the second rotating disk (215) blocks the liquid outlet groove (123) of the annular partition (122) to prevent the culture medium from being discharged. By starting the hydraulic cylinder (324), the hydraulic cylinder (324) drives the grabbing rod (326) to expand and contract, and the arc groove (112) on the surface of the bottom plate (111) limits the liquid discharge. The rubber sheet (321) and the mounting plate (322) are forced to rotate and lift at the same time, and since the top of the auxiliary cover plate (421) contacts the bottom of the sealing cover plate (51), the movement of the sealing box (325) forces the piston (414) to perform a rotational lifting movement, so that the culture medium inside the placement vessel (121) enters the interior of the culture vessel (411) through the liquid transport pipe (412), penetrates the bladder cancer tumor cells, and thus cultivates the bladder cancer tumor cells; S4: When the culture medium needs to be replaced after a period of time or when the culture medium is observed through the observation port (52), the rotating motor (132) is started to drive the gear (133) and the first rotating disk (211) to rotate, thereby rotating the second rotating disk (215). Under the action of the return spring (214), when the blocking plate (216) rotates and separates from the liquid outlet trough (123), the second rotating disk (215) sinks, so that the blocking plate (216) smoothly separates from the liquid outlet trough (123). At this time, the second rotating disk (215) is released. Excessive culture medium inside the placement vessel (121) falls into the interior of the drainage plate (221) through the first rotating disk (211) and the second rotating disk (215), and is discharged through the drainage pipe (222). After being drained clean, the blocking plate (216) and the liquid outlet trough (123) are restored to their initial positions, and the culture medium to be replaced is then injected into the interior of the placement vessel (121) through the injection valve (53). At this time, the hydraulic cylinder (324) is started again to allow the new culture medium to interact and mix with the culture medium inside the culture vessel (411); S5: After the culture is completed, the culture liquid is drained cleanly by driving the hydraulic cylinder (324), and then the culture vessel (411) is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel (411).

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