In-situ biological detection system and detection method based on plug-in Raman probe

Abstract

The application discloses an in-situ biological detection system and method based on an insertion type Raman probe, and the in-situ biological detection system comprises a Raman detection device and a biological detection cabin. The Raman detection device comprises a Raman probe, and the Raman probe is provided with a probe head which is inserted into the biological detection cabin. The biological detection cabin comprises a shell and a biological reaction substrate arranged in the shell, and the shell is provided with a drain hole. After the in-situ biological detection system is assembled, the system is dived to a required position, and the metabolic interaction process between the biological detection cabin and the in-situ environment is monitored in real time and continuously by the Raman detection device. The in-situ biological detection system based on the insertion type Raman probe adopts the in-situ Raman detection method, and has a simpler structure and stable deep-sea in-situ detection performance. Since the system itself is used for in-situ environment detection, the application needs of sensing systems for in-situ detection of various types of organisms can be met, and the system has more sensitive selectivity.

Description

Technical Field

[0001] This invention relates to the field of deep-sea exploration equipment technology, and more specifically to an in-situ biological detection system and method based on an insertable Raman probe. Background Technology

[0002] In recent years, with the gradual development of various detection technologies for deep-sea exploration, Raman spectroscopy has gained popularity due to its advantages such as non-invasiveness, high sensitivity, high selectivity, and rapid analysis. Deep-sea in-situ Raman spectroscopy is a technique that uses laser irradiation of samples and measures changes in the intensity of scattered light to analyze the chemical composition and structure of deep-sea samples. The applications of deep-sea in-situ Raman spectroscopy are very broad, including deep-sea geological research, deep-sea resource exploration, and deep-sea environmental monitoring. However, it still has certain limitations in the study of deep-sea microorganisms. Due to the harsh deep-sea environment and the diverse types of microorganisms, the weak in-situ microbial spectral signals are difficult to analyze effectively. Effective real-time in-situ detection of the metabolic processes of deep-sea microorganisms would have significant scientific reference value for multiple disciplines. Therefore, deep-sea in-situ microbial Raman spectroscopy is a problem that urgently needs to be solved in this field. Summary of the Invention

[0003] In view of this, the present invention provides an in-situ biological detection system and method based on an insertable Raman probe, which has more sensitive selectivity.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] Firstly, this invention provides an in-situ biological detection system based on an insertable Raman probe, comprising: a Raman detection device and a biological detection chamber;

[0006] The Raman detection device includes a Raman probe, the Raman probe having a probe inserted into the biological detection chamber;

[0007] The biological detection chamber includes an outer shell and a biological reaction substrate placed inside the outer shell, and the surface of the outer shell is provided with drainage holes.

[0008] Preferably, the Raman detection device is further provided with a light source coupling port and a collimating optical path, and the detection light window of the Raman probe is a high-transmittance window.

[0009] Preferably, the inner wall of the top of the outer casing is provided with a fastening slot, and the outer wall of the probe is provided with a protrusion that matches the fastening slot.

[0010] Preferably, the inner wall of the outer casing is provided with a fixing mesh.

[0011] Preferably, the bioreaction substrate is encapsulated using a bio-semi-permeable membrane.

[0012] Furthermore, the semipermeable membrane is one or more of animal membranes, cellophane, cellulose derivatives, and synthetic polymer membranes.

[0013] Furthermore, the synthetic polymer film is one or more of polysulfone, polyethersulfone, polyamide, and polyvinylidene fluoride.

[0014] The detection method using the in-situ biological detection system described above includes the following steps:

[0015] After assembling the in-situ biological detection system, the device is submerged to the required location and uses Raman spectroscopy to continuously monitor the metabolic interaction between the organisms inside the biological detection chamber and the in-situ environment in real time.

[0016] As can be seen from the above technical solution, compared with the prior art, the present invention discloses an in-situ biological detection system and detection method based on an insertion Raman probe, which has the following beneficial effects:

[0017] This insertable Raman probe in-situ biological detection system adopts the Raman in-situ detection method, which has a simpler structure and stable performance in deep-sea in-situ detection. Since the system itself is an in-situ environmental detection system, it can meet the application needs of various biological in-situ detection sensor systems, making it more sensitive and selective. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of the Raman detector.

[0020] Figure 2 This is a structural diagram of the biological detection cabin;

[0021] Figure 3 This is a cross-sectional view of the biological detection cabin;

[0022] Figure 4 This is a schematic diagram of an optical signal passing through a Raman detector.

[0023] Figure 5 This is a schematic diagram showing the structural matching between the probe and the housing.

[0024] In the figure, 111-Raman probe, 112-Biodetection chamber, 113-Protrusion; 211-Shell shell, 212-Fastening bayonet, 213-Drainage hole, 311-Fixing net, 312-Bioreaction substrate, 411-Glass device, 511-Optical window. Detailed Implementation

[0025] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0026] As attached Figure 1-3 As shown, the in-situ biodetection system based on an insertion Raman probe includes: a Raman detection device ( Figure 1 ) and biological detection cabin 112 ( Figure 1 and 2 -3);

[0027] The Raman detection device includes a Raman probe 111, which has a probe that is inserted into the biological detection chamber;

[0028] The biological detection chamber 112 includes an outer shell 211 and a biological reaction substrate 312 placed inside the outer shell 211. The surface of the outer shell 211 is provided with drainage holes 213. The drainage holes 213 can accelerate the flow and discharge of seawater when used in situ, so as to ensure the decompression of the water in the biological detection chamber and the interaction between the organisms in the chamber and the in situ environment.

[0029] The Raman detector is equipped with a light source coupling port and a collimating optical path. Figure 4 The sample is provided with a glass device 411 and an optical window 511. The optical window 511 is a high-transmittance optical window. The light signal passes through the optical coupling and collimation optical path of the glass device 411 in the Raman probe in sequence, and then the excitation light is irradiated onto the sample through the high-transmittance optical window 511.

[0030] The inner wall of the top of the outer casing 211 is provided with a fastening slot 212. Figure 2 and 5 The probe has a protrusion 113 on its outer wall, which matches the fastening slot 212 to prevent it from falling off or loosening.

[0031] The inner wall of the outer shell 211 is provided with a fixing net 311 to protect and fix the biological reaction substrate 312.

[0032] The bioreactor substrate 312 is an internal material encapsulated by a biological semi-permeable membrane, ensuring that the analyte and some metabolic products are not lost within the membrane. The semi-permeable membrane can be one or more of animal membranes, cellophane, cellulose derivatives, and synthetic polymer membranes. The synthetic polymer membrane can be one or more of polysulfone, polyethersulfone, polyamide, and polyvinylidene fluoride.

[0033] When using the Raman probe in-situ biodetection system, the Raman detector is connected to the biodetection chamber, which contains the observation samples needed for deep-sea in-situ detection. Before the equipment dives, the bioreactor substrate, biodetection chamber, and Raman detector are assembled sequentially. Once the equipment has descended to a certain environment and begun operation, the Raman detector can continuously monitor the metabolic interactions between the organisms within the bioreactor and the in-situ environment in real time.

[0034] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0035] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An in-situ biological detection system based on an insertion Raman probe, characterized in that, include: Raman spectroscopy device and biological detection chamber; The Raman detection device includes a Raman probe, the Raman probe having a probe inserted into the biological detection chamber; The biological detection chamber includes an outer shell and a biological reaction substrate placed inside the outer shell, and the surface of the outer shell is provided with drainage holes; The inner wall of the outer shell is provided with a fixing net, and the bioreactor substrate is wrapped with a bio-semi-permeable membrane; The detection method of the in-situ biological detection system includes the following steps: After assembling the in-situ biological detection system, the device is submerged to the required location and uses Raman spectroscopy to continuously monitor the metabolic interaction between the organisms inside the biological detection chamber and the in-situ environment in real time.

2. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that, The Raman detection device is also equipped with a light source coupling port and a collimated optical path, and the detection light window of the Raman probe is a high-transmittance window.

3. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that, The inner wall of the top of the outer casing is provided with a fastening slot, and the outer wall of the probe is provided with a protrusion that matches the fastening slot.

4. The in-situ biological detection system based on an insertion Raman probe according to claim 1, characterized in that, The semipermeable membrane is one or more of animal membranes, cellophane, cellulose derivatives, and synthetic polymer membranes.

5. The in-situ biological detection system based on an insertion Raman probe according to claim 4, characterized in that, The synthesized polymer film is one or more of polysulfone, polyethersulfone, polyamide, and polyvinylidene fluoride.

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