A detection and analysis device for immune protein

Abstract

The present invention relates to the field of protein detection technology, and in particular to a detection and analysis device for immune protein. It includes: a frame, the frame is fixedly connected to an ice bath shell, the ice bath shell is filled with a heat-conducting medium, and the frame is installed with a detection analyzer through a mounting frame; a vertical frame, fixedly connected to the ice bath shell, the vertical frame is installed with a hydraulic push rod; a placement frame, arranged at the telescopic end of the hydraulic push rod, the placement frame is used to place a transfer frame; a refrigeration mechanism, arranged on the frame, and used to refrigerate the heat-conducting medium in the ice bath shell. The present invention uses the heat-conducting medium in the ice bath shell to fully cover the transfer frame for heat dissipation, avoiding the existing heat dissipation method of only using a few ice bags to contact the transfer frame, and dissipating the contact area of ​​the transfer frame, while the ice bags cannot completely cover the transfer frame, resulting in uneven heat dissipation, reducing the transfer effect of the immune protein, thereby reducing the accuracy of the immune protein detection results.

Description

Technical Field

[0001] The present invention relates to the technical field of protein detection, and in particular to a detection and analysis device for immune protein. Background Art

[0002] The immune protein detection and analysis device is a detection device used to detect the concentration of various immune proteins in the human body. It can detect indicators such as immunoglobulins, complement, cytokines in blood, urine or other body fluids, and is widely used in clinical diagnosis, disease monitoring, drug efficacy evaluation and other fields. Its detection methods and experimental means mainly use Real-time and WB to detect the gene and protein expression of key molecules in the lipid metabolism signaling pathway of gastric cancer cells after FASN overexpression, and use the luciferase transcription reporter system to detect the regulatory effect of FASN on the transcriptional activity of key mitochondrial functional genes.

[0003] The WB detection process consists of protein extraction, protein concentration determination, electrophoresis, transfer, blocking, primary antibody incubation and secondary antibody incubation. The transfer needs to be performed in a low temperature environment to dissipate the heat generated during the transfer process. The existing transfer is performed by simply contacting the transfer frame with an ice bag to dissipate the heat in the contact area of ​​the transfer frame, but the ice bag cannot completely cover the transfer frame, and the transfer frame has uneven heat dissipation, which reduces the transfer effect of the immune protein and thus reduces the accuracy of the immune protein detection results. Summary of the invention

[0004] In order to overcome the shortcomings mentioned in the above technical background, the present invention provides a detection and analysis device for immune protein.

[0005] The technical solution of the present invention is: a detection and analysis device for immune protein, comprising: a frame, the frame is fixedly connected to an ice bath shell, the ice bath shell is filled with a heat-conducting medium, and the frame is installed with a detection analyzer through a mounting frame; a vertical frame, fixedly connected to the ice bath shell, the vertical frame is installed with a hydraulic push rod; a placement frame, arranged at the telescopic end of the hydraulic push rod, the placement frame is used to place a transfer rack; a refrigeration mechanism, arranged on the frame, and used to cool the heat-conducting medium in the ice bath shell.

[0006] Preferably, the refrigeration mechanism includes: a connecting shaft, which is arranged on the ice bath shell, the connecting shaft is fixedly connected to an intermediate tube, and the intermediate tube is located in the ice bath shell; a first shell and a second shell, both of which are arranged on the connecting shaft, the first shell is fixedly connected and connected to a first tube, the second shell is fixedly connected and connected to a second tube, the first tube is connected to an external refrigeration device, the second tube is connected to an external circulation device, and the external refrigeration device is connected to an external circulation device.

[0007] Preferably, the intermediate tube is in a planar spiral shape and has elastic deformation capability, so as to increase the contact area with the heat-conducting medium in the ice bath shell.

[0008] Preferably, it also includes: a motor installed on the frame, the output shaft of the motor is fixedly connected to the connecting shaft, the connecting shaft is rotatably connected to the ice bath shell, the first shell and the second shell are both rotatably connected to the connecting shaft, a first limit block is fixedly connected in the ice bath shell, and the first limit block is used to squeeze the intermediate tube; an extrusion assembly is arranged on the placement rack for moving the transfer rack; two clamping assemblies are arranged in a mirror image and are both arranged on the placement rack for stably clamping the transfer rack.

[0009] Preferably, the extrusion assembly includes: a fixed ring fixedly connected to the placement rack, the fixed ring fixedly connected with a circumferentially arranged second limit block; a connecting rod fixedly connected to the connecting shaft, the connecting rod provided with a first ball, the first ball being used to extrude the adjacent second limit block; a guide assembly arranged in the ice bath shell, for guiding the heat-conducting medium in the ice bath shell; a rotating assembly arranged on the placement rack, for causing the placement rack to reciprocate in the ice bath shell.

[0010] Preferably, the second limit block is a "right triangle" for changing the motion state of the fixing ring.

[0011] Preferably, the clamping assembly includes: a guide shell, slidably connected to the placement rack, the guide shell is slidably connected to a sliding rod, the sliding rod is provided with a second ball, a first elastic element is fixedly connected between the guide shell and the sliding rod, a squeezing pad is fixedly connected to the side of the guide shell away from the second ball, the squeezing pad is fixedly connected to the placement rack, the squeezing pad is used to squeeze the transfer rack, and the squeezing pad is made of elastic material; a limiting plate, fixedly connected to the ice bath shell, the limiting plate is an arc-shaped plate, and the limiting plate is used to squeeze the second ball.

[0012] Preferably, the guide assembly comprises: a guide shell fixedly connected to the ice bath shell, the guide shell being an arc-shaped shell; an arc-shaped rod fixedly connected to the connecting rod, the cross-section of the arc-shaped rod being a "right triangle" for squeezing the heat-conducting medium in adjacent areas in the ice bath shell.

[0013] Preferably, the guide shell is provided with through holes arranged at intervals to allow the heat-conducting medium in the ice bath shell to pass through.

[0014] Preferably, the rotating assembly includes: a fixing part, fixedly connected to the placing frame, the limit plate is a part of the spherical shell; a connecting frame, rotatably connected to the fixing part; a movable shell, fixedly connected to the connecting frame, the movable shell is slidably connected to the telescopic end of the hydraulic push rod, and a second elastic element is fixedly connected between the fixing part and the movable shell; a limit ring, fixedly connected to the telescopic end of the hydraulic push rod, and the limit ring is spline-connected to the movable shell.

[0015] Compared with the prior art, the present invention has the following advantages: the present invention uses the heat-conducting medium in the ice bath shell to fully cover the transfer frame for heat dissipation, thereby avoiding the existing heat dissipation method of only using a number of ice bags to contact the transfer frame to dissipate heat in the contact area of ​​the transfer frame, while the ice bags cannot completely cover the transfer frame, resulting in uneven heat dissipation, reducing the transfer effect of the immune protein, thereby reducing the accuracy of the immune protein detection result; the first limit block is used to squeeze the middle tube, so that the squeezed middle tube is deformed, and then the middle tube is quickly reset under the action of its own elastic force, thereby generating a vibration The first ball is used to squeeze the second limit block, so that the placement rack drives the transfer rack to move in the ice bath shell, thereby increasing the degree of confusion between the heat-conducting medium and the transfer rack during contact, and improving the ice bath effect on the transfer rack. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0017] Figure 2 It is a three-dimensional structural cross-sectional view of the frame and the ice bath shell of the present invention;

[0018] Figure 3 It is a three-dimensional structural cross-sectional view of the ice bath shell and the guide shell of the present invention;

[0019] Figure 4 It is a three-dimensional structural cross-sectional view of the connecting shaft, the first shell and the second shell of the present invention;

[0020] Figure 5 It is a sectional view of the three-dimensional structure of the placement rack of the present invention when it moves;

[0021] Figure 6 For the present invention Figure 3 A magnified view of the three-dimensional structure at center A;

[0022] Figure 7 For the present invention Figure 5 A magnified view of the three-dimensional structure at B in the middle;

[0023] Figure 8 It is a three-dimensional structural exploded view of the fixing member and the parts thereon of the present invention.

[0024] Markings in the accompanying drawings: 101, transfer rack, 1, rack body, 2, ice bath shell, 3, detection analyzer, 4, stand, 5, hydraulic push rod, 6, placement rack, 701, connecting shaft, 702, intermediate tube, 703, first shell, 704, second shell, 705, first tube, 706, second tube, 801, motor, 802, first limit block, 901, fixing ring, 902, second limit block, 903, connection Rod, 904, first ball, 1001, guide shell, 1002, sliding rod, 1003, second ball, 1004, first elastic element, 1005, extrusion pad, 1006, limit plate, 1101, guide shell, 1102, arc rod, 1103, through hole, 1201, fixing part, 1202, connecting frame, 1203, movable shell, 1204, second elastic element, 1205, limit ring. DETAILED DESCRIPTION

[0025] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation mode of the present invention is described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are elaborated to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein. Those skilled in the art can make similar improvements without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0026] The transcriptional activity luciferase reporter system detection is a rapid and highly sensitive method. The basic principle is to connect the gene fragment containing the binding site of the transcription factor to the firefly luciferase coding gene, construct a plasmid expression vector, transfect the vector into the cell, and activate the reporter gene to express luciferase. By detecting the enzyme, it can be determined whether the factor activates the transcription factor. In this study, different transcriptional activity luciferase reporter systems were constructed according to the promoter sequences of different transcription factors, and the dual reporter gene detection system was used to determine its signal transduction activity.

[0027] like Figure 1-Figure 3As shown, an embodiment of the present invention is proposed, which provides a detection and analysis device for immune protein to solve the problem that it is difficult for existing detection and analysis devices to fully and evenly cool the transfer rack, so that the immune protein loses its activity due to heat generated by electronic equipment during the transfer process, resulting in inaccurate detection and analysis results. The device comprises: a frame 1, the frame 1 is fixedly connected to an ice bath shell 2, the ice bath shell 2 is filled with a heat-conducting medium, and the frame 1 is equipped with a detection analyzer 3 through a mounting frame; a stand 4 is fixedly connected to the ice bath shell 2, and the stand 4 is equipped with a hydraulic push rod 5; a placement frame 6 is arranged at the telescopic end of the hydraulic push rod 5, and the placement frame 6 is used to place the transfer rack 101; a refrigeration mechanism is arranged on the frame 1, and is used to refrigerate the heat-conducting medium in the ice bath shell 2.

[0028] In the above scheme, the transfer rack 101 is a prior art, and the upper side of the transfer rack 101 is provided with positive and negative electrode connectors for connecting to an external power source (its detailed working principle will not be described in detail), and the detection analyzer 3 is an existing device, and its detailed working principle will not be described in detail. The above scheme mainly uses Real-time and WB to detect the gene and protein expression of key molecules in the lipid metabolism signaling pathway of gastric cancer cells after FASN overexpression, and uses the luciferase transcription reporter system to detect the regulatory effect of FASN on the transcriptional activity of key mitochondrial functional genes. The principle is to use anti-FASN as a bait protein (combined with IP technology to determine the target molecule that directly interacts with FASN), and then use WB to detect the expression of pMET, RhoGTPase signaling pathway related protein molecules to determine the protein molecules that interact with FASN. The transfer rack 101 is used to carry the immune protein that needs to be transferred, the heat conduction medium in the ice bath shell 2 is water, the hydraulic push rod 5 is connected to the external hydraulic system, and the lower part of the placement rack 6 is provided with a rectangular groove to increase the stability of the transfer rack 101 after placement. When using the device, the staff places the transfer frame 101 in the groove at the bottom of the placement frame 6, and then uses the telescopic end of the hydraulic push rod 5 to drive the placement frame 6 to move downward, and the placement frame 6 drives the transfer frame 101 to move downward together, and the transfer frame 101 gradually immerses in the (ice) water in the ice bath shell 2 until the transfer frame 101 is submerged by the (ice) water in the ice bath shell 2, and then the placement frame 6 stops moving downward, so as to evenly dissipate the heat of the transfer frame 101, avoiding the existing heat dissipation method of only using a number of ice bags to contact the transfer frame 101, and the contact area of ​​the transfer frame 101 is The transfer rack 101 is cooled by the ice pack, and the ice bag cannot completely cover the transfer rack 101, resulting in uneven heat dissipation, which reduces the transfer effect of the immune protein and thus reduces the accuracy of the immune protein detection result. The (ice) water in the ice bath shell 2 is then continuously cooled by the refrigeration mechanism to ensure uniform heat dissipation of the transfer rack 101. After the transfer is completed, the staff moves the transfer rack 101 out of the ice bath shell 2 through the telescopic end of the hydraulic push rod 5, and then removes the transfer rack 101 from the placement rack 6, and then uses the detection analyzer 3 to detect and analyze the transferred immune protein.

[0029] like Figure 2-Figure 5As shown, the refrigeration mechanism includes: a connecting shaft 701, which is arranged on the ice bath shell 2, and the connecting shaft 701 is fixedly connected with an intermediate tube 702, and the intermediate tube 702 is located in the ice bath shell 2, and the intermediate tube 702 is a plane spiral shape and has elastic deformation ability, and is used to increase the contact area with the heat-conducting medium in the ice bath shell 2; a first shell 703 and a second shell 704, which are both arranged on the connecting shaft 701, the first shell 703 is fixedly connected and connected with a first tube 705, and the second shell 704 is fixedly connected and connected with a second tube 706, the first tube 705 is connected to an external refrigeration device, the second tube 706 is connected to an external circulation device, and the external refrigeration device is connected to an external circulation device.

[0030] In the above scheme, the intermediate tube 702 is a stainless steel thin-walled tube, and the intermediate tube 702 is used to fill a refrigerant (the refrigerant includes but is not limited to Freon) to cool the heat-conducting medium in the ice bath shell 2. The refrigerant medium circulates in the intermediate tube 702 by using external circulation equipment and refrigeration equipment, that is, the refrigerant medium enters the first shell 703 through the first tube 705, and then enters the intermediate tube 702, so as to indirectly contact the heat-conducting medium in the ice bath shell 2 and cool the heat-conducting medium in the ice bath shell 2, so as to ensure that the temperature of the heat-conducting medium in the ice bath shell 2 is always the temperature required for the ice bath of the transfer rack 101, thereby improving the stability of the immune protein transfer during the ice bath of the transfer rack 101, and thereby improving the accuracy of subsequent immune protein detection and analysis.

[0031] like Figure 3 As shown, it also includes: a motor 801, which is installed on the frame 1, the output shaft of the motor 801 is fixedly connected to the connecting shaft 701, the connecting shaft 701 is rotatably connected to the ice bath shell 2, the first shell 703 and the second shell 704 are both rotatably connected to the connecting shaft 701, and a first limit block 802 is fixedly connected in the ice bath shell 2, and the first limit block 802 is used to squeeze the intermediate tube 702; an extrusion component is arranged on the placement frame 6, which is used to move the transfer frame 101; and two clamping components are arranged in a mirror image, which are both arranged on the placement frame 6 and are used to stably clamp the transfer frame 101.

[0032] In the above scheme, the first limit block 802 is a "right-angled triangular prism", and the inclined surface is used to squeeze the intermediate tube 702, so that the intermediate tube 702 undergoes elastic deformation. When the transfer film frame 101 is placed in an ice bath, the output shaft of the motor 801 drives the connecting shaft 701 to rotate counterclockwise (such as Figure 3Taking the top view as an example), the connecting shaft 701 and the ice bath shell 2, the first shell 703 and the second shell 704 all rotate relative to each other, and the connecting shaft 701 drives the intermediate tube 702 to rotate counterclockwise together, thereby changing the position of the intermediate tube 702 in the ice bath shell 2, thereby continuously changing the contact position between the intermediate tube 702 and the heat-conducting medium in the ice bath shell 2, so that the heat-conducting medium in the plane where the intermediate tube 702 is located is evenly cooled, thereby improving the heat exchange efficiency of the two, that is, improving the refrigeration efficiency of the heat-conducting medium in the ice bath shell 2, so that the temperature of the heat-conducting medium in the ice bath shell 2 is always in a relatively stable area, thereby stably ice-bathing the transfer rack 101, thereby improving the transfer of immune proteins. Quality, during the counterclockwise rotation of the middle tube 702, when the middle tube 702 contacts the first limit block 802, the middle tube 702 is squeezed and deformed by the first limit block 802, and then after the two are separated, the middle tube 702 quickly resets under the action of its own elastic force, thereby generating vibration and impacting the heat-conducting medium, and finally transmitting the impact to the transfer frame 101, so that the heat-conducting medium and the transfer frame 101 are "fully contacted", improving the ice bath effect of the transfer frame 101, and using the extrusion component to make the transfer frame 101 move in the ice bath shell 2, so that the transfer frame 101 dissipates heat evenly in the ice bath shell 2, and the transfer frame 101 is clamped and stabilized by the clamping component.

[0033] like Figure 3 and Figure 5 As shown, the extrusion assembly includes: a fixed ring 901, fixedly connected to the placement rack 6, the fixed ring 901 is fixedly connected with a circumferentially arranged second limit block 902, the second limit block 902 is a "right-angled triangle", and is used to change the movement state of the fixed ring 901; a connecting rod 903, fixedly connected to the connecting shaft 701, the connecting rod 903 is provided with a first ball 904, and the first ball 904 is used to extrude the adjacent second limit block 902; a guiding assembly, arranged in the ice bath shell 2, for guiding the heat-conducting medium in the ice bath shell 2; a rotating assembly, arranged in the placement rack 6, for causing the placement rack 6 to reciprocate in the ice bath shell 2.

[0034] In the above scheme, the first ball 904 rolls in the connecting rod 903 to reduce the friction between the two. During the downward movement of the placement rack 6, the placement rack 6 drives the second limit block 902 to move downward together through the fixing ring 901 until the placement rack 6 stops moving downward. At this time, the inclined surface of the second limit block 902 contacts the first ball 904. During the counterclockwise rotation of the connecting shaft 701, the connecting shaft 701 drives the connecting rod 903 to rotate counterclockwise, and the connecting rod 903 drives the first ball 904 to rotate counterclockwise. The first ball 904 begins to squeeze the inclined surface of the second limit block 902, and the second limit block 902 begins to rotate counterclockwise. The second limit block 902 drives the placement rack 6 to rotate counterclockwise through the fixing ring 901 (due to the limitation of the rotating component, the fixing ring 901 always rotates relative to the connecting rod 903 during the process), thereby disturbing the heat-conducting medium in the adjacent areas of the ice bath shell 2, increasing the disorder of the heat-conducting medium, that is, improving its temperature stability, and then improving the transfer quality of the immune protein on the transfer rack 101, and then the first ball 904 passes through the second limit block 902 through the rotating component, and then the placement rack 6 is reset, and the guide component is used to guide the heat-conducting medium in the ice bath shell 2, so that the temperature of the heat-conducting medium in each area of ​​the ice bath shell 2 is relatively stable.

[0035] like Figure 5 and Figure 6 As shown, the clamping assembly includes: a guide shell 1001, which is slidably connected to the placement rack 6, the guide shell 1001 is slidably connected to a sliding rod 1002, the sliding rod 1002 is provided with a second ball 1003, a first elastic element 1004 is fixedly connected between the guide shell 1001 and the sliding rod 1002, a squeezing pad 1005 is fixedly connected to the side of the guide shell 1001 away from the second ball 1003, the squeezing pad 1005 is fixedly connected to the placement rack 6, the squeezing pad 1005 is used to squeeze the transfer rack 101, and the squeezing pad 1005 is made of elastic material; a limiting plate 1006, which is fixedly connected to the ice bath shell 2, the limiting plate 1006 is an arc-shaped plate, and the limiting plate 1006 is used to squeeze the second ball 1003.

[0036] In the above scheme, the first elastic element 1004 is a spring, which is used to apply elastic force to the guide shell 1001. The fixed connection area between the squeezing pad 1005 and the placement rack 6 is the contact area between the two. The squeezing pad 1005 can be elastically deformed, and the distance between the limiting plate 1006 and the inner wall of the ice bath shell 2 gradually increases from top to bottom. In the process of the placement rack 6 moving downward, the placement rack 6 drives the guide shell 1001 and the squeezing pad 1005 to move downward together, the guide shell 1001 drives the sliding rod 1002 and the first elastic element 1004 to move downward together, and the sliding rod 1002 drives the second ball 1003 to move downward together. After the second ball 1003 moves downward until it contacts the limiting plate 1006, as the second ball 1003 continues to move downward, the second ball 1003 is squeezed by the limiting plate 1006 and begins to move toward the side close to the placement rack 6, and the second ball 1003 drives the sliding rod 1002 to move together, and the sliding rod 1002 drives the guide shell 1001 to move together through the first elastic element 1004, and the guide shell 1001 squeezes the squeezing pad 1005, and the squeezing pad 1005 is deformed and gradually applies squeezing force to the transfer rack 101, so as to clamp and fix the transfer rack 101, so that the transfer rack 101 remains stable during the transfer process, thereby improving the transfer quality of the immune protein.

[0037] like Figure 2 and Figure 3 As shown, the guide assembly includes: a guide shell 1101, which is fixedly connected to the ice bath shell 2, and the guide shell 1101 is a part of the spherical shell; an arc rod 1102, which is fixedly connected to the connecting rod 903, and the cross section of the arc rod 1102 is a "right triangle", and the hypotenuse faces the guide shell 1101, which is used to squeeze the heat-conducting medium in the adjacent area of ​​the ice bath shell 2, and the guide shell 1101 is provided with through holes 1103 arranged at intervals for the heat-conducting medium in the ice bath shell 2 to pass through.

[0038] In the above scheme, the arc rod 1102 is "spiral-shaped". During the counterclockwise rotation of the connecting rod 903, the connecting rod 903 drives the arc rod 1102 to rotate counterclockwise together. During the process, the inclined surface of the arc rod 1102 squeezes the heat-conducting medium in the adjacent area of ​​the ice bath shell 2, and the squeezed heat-conducting medium begins to move toward the direction close to the guide shell 1101. Then, the heat-conducting medium is blocked and guided by the guide shell 1101, that is, the heat-conducting medium begins to move upward along the inside of the guide shell 1101, so that the heat-conducting medium moves in the ice bath shell 2 (the heat-conducting medium located at the upper part of the ice bath shell 2). The medium starts to move downward from the outside of the ice bath shell 2, that is, the heat-conducting medium in the ice bath shell 2 circulates), so that the heat-conducting medium in the ice bath shell 2 is evenly contacted with the intermediate tube 702, so that the temperature of the heat-conducting medium in the ice bath shell 2 is even, thereby improving the ice bath effect on the transfer membrane rack 101. During the process, a part of the heat-conducting medium squeezed by the arc rod 1102 passes through the through hole 1103 and impacts the heat-conducting medium in the adjacent area (the area outside the ice bath shell 2) in the form of a "jet", so that the heat-conducting medium in the circulation process is further disturbed, thereby improving the uniformity of cooling the heat-conducting medium.

[0039] like Figure 3 , Figure 5-Figure 7 and Figure 8 As shown, the rotating assembly includes: a fixing member 1201, fixedly connected to the placement frame 6, and a limit plate 1006 is a part of the spherical shell; a connecting frame 1202, rotatably connected to the fixing member 1201; a movable shell 1203, fixedly connected to the connecting frame 1202, the movable shell 1203 is slidably connected to the telescopic end of the hydraulic push rod 5, and a second elastic element 1204 is fixedly connected between the fixing member 1201 and the movable shell 1203; a limit ring 1205, fixedly connected to the telescopic end of the hydraulic push rod 5, and the limit ring 1205 is spline-connected to the movable shell 1203.

[0040] In the above solution, during the process of the first ball 904 squeezing the upper inclined surface of the second stop block 902, the force applied by the first ball 904 to the second stop block 902 can be divided into a vertical upward force and a counterclockwise torsional force (such as Figure 5Taking the top view as an example), when the first ball 904 squeezes the second limit block 902, the second limit block 902 drives the placement frame 6 to rotate counterclockwise through the fixing ring 901 (the second ball 1003 rolls along the limit plate 1006 during the process), and the placement frame 6 drives the fixing piece 1201 to rotate counterclockwise together, and the fixing piece 1201 and the connecting frame 1202 rotate relative to each other, and the second elastic element 1204 is deformed. As the torque applied by the second elastic element 1204 to the fixing piece 1201 gradually increases, the effect of the circumferential component of the first ball 904 on the second limit block 902 gradually decreases (that is, the second limit block 902 gradually stops rotating with the first ball 904), that is, the upward force applied by the first ball 904 to the second limit block 902 gradually increases, and the upward force Gradually overcoming gravity causes the placement rack 6 to gradually move upward, and the placement rack 6 drives the movable shell 1203 to move upward together through the fixing member 1201 and the connecting frame 1202, and the movable shell 1203 slides relatively with the limiting ring 1205. As the first ball 904 continues to rotate counterclockwise, when the first ball 904 is disengaged from the second limiting block 902, the fixing member 1201 drives the placement rack 6 to reset (rotate clockwise) under the action of the torsion of the second elastic element 1204, and the placement rack 6 and the parts thereon move downward (reset) under the action of their own gravity, so that the transfer rack 101 moves in the heat-conducting medium in the ice bath shell 2, thereby increasing the confusion when the heat-conducting medium and the transfer rack 101 are in contact, and improving the ice bath effect on the transfer rack 101.

[0041] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A detection and analysis device for immune protein, characterized in that: Included are: A frame (1), the frame (1) being fixedly connected to an ice bath shell (2), the ice bath shell (2) being filled with a heat-conducting medium, and the frame (1) being mounted with a detection analyzer (3) via a mounting frame; A stand (4) fixedly connected to the ice bath shell (2), the stand (4) being provided with a hydraulic push rod (5); A placement rack (6), arranged at the telescopic end of the hydraulic push rod (5), the placement rack (6) being used to place the transfer rack (101); A refrigeration mechanism, arranged on the frame (1), and used for refrigerating the heat-conducting medium in the ice bath shell (2); The refrigeration mechanism comprises: A connecting shaft (701) is arranged on the ice bath shell (2); the connecting shaft (701) is fixedly connected to an intermediate tube (702); the intermediate tube (702) is located inside the ice bath shell (2); the intermediate tube (702) is used to fill a refrigerant medium; external circulation equipment and refrigeration equipment are used to cause the refrigerant medium to circulate in the intermediate tube (702); and the intermediate tube (702) has elastic deformation capability; The first shell (703) and the second shell (704) are both arranged on the connecting shaft (701); the first shell (703) is fixedly connected to and communicated with a first tube (705); the second shell (704) is fixedly connected to and communicated with a second tube (706); the first tube (705) is connected to an external refrigeration device; the second tube (706) is connected to an external circulation device; and the external refrigeration device is connected to an external circulation device; Also included are: a motor (801) mounted on the frame (1); an output shaft of the motor (801) being fixedly connected to the connecting shaft (701); the connecting shaft (701) being rotationally connected to the ice bath shell (2); the first shell (703) and the second shell (704) being rotationally connected to the connecting shaft (701); a first limit block (802) being fixedly connected inside the ice bath shell (2); the first limit block (802) being used to squeeze the intermediate tube (702); An extrusion assembly, arranged on the placement rack (6), and used to move the transfer rack (101); The two clamping assemblies are arranged in a mirror image and are both provided on the placement rack (6) and are used to stably clamp the transfer rack (101).

2. A detection and analysis device for immune protein according to claim 1, characterized in that: The intermediate tube (702) is in a planar spiral shape and has elastic deformation capability, and is used to increase the contact area with the heat-conducting medium in the ice bath shell (2).

3. A detection and analysis device for immune protein according to claim 1, characterized in that: The extrusion assembly comprises: A fixing ring (901) fixedly connected to the placement rack (6), the fixing ring (901) being fixedly connected to a second limiting block (902) arranged in a circumferential direction; A connecting rod (903) fixedly connected to the connecting shaft (701), the connecting rod (903) being provided with a first ball (904), the first ball (904) being used to squeeze the adjacent second limiting block (902); A guide component, arranged in the ice bath shell (2), and used for guiding the heat-conducting medium in the ice bath shell (2); A rotating assembly is arranged on the placement rack (6) and is used to enable the placement rack (6) to reciprocate in the ice bath shell (2).

4. A detection and analysis device for immune protein according to claim 3, characterized in that: The second limit block (902) is a "right triangle" and is used to change the movement state of the fixing ring (901).

5. The detection and analysis device for immune protein according to claim 3, characterized in that: The clamping assembly comprises: A guide shell (1001) is slidably connected to the placement rack (6); the guide shell (1001) is slidably connected to a sliding rod (1002); the sliding rod (1002) is provided with a second ball (1003); a first elastic element (1004) is fixedly connected between the guide shell (1001) and the sliding rod (1002); a squeezing pad (1005) is fixedly connected to a side of the guide shell (1001) away from the second ball (1003); the squeezing pad (1005) is fixedly connected to the placement rack (6); the squeezing pad (1005) is used to squeeze the transfer rack (101); and the squeezing pad (1005) is made of elastic material; A limiting plate (1006) is fixedly connected to the ice bath shell (2); the limiting plate (1006) is an arc-shaped plate; the limiting plate (1006) is used to squeeze the second ball (1003).

6. The detection and analysis device for immune protein according to claim 3, characterized in that: The guide component comprises: A guide shell (1101) is fixedly connected to the ice bath shell (2), and the guide shell (1101) is an arc-shaped shell; The arc-shaped rod (1102) is fixedly connected to the connecting rod (903); the cross section of the arc-shaped rod (1102) is a "right triangle" and is used to squeeze the heat-conducting medium in adjacent areas within the ice bath shell (2).

7. A detection and analysis device for immune protein according to claim 6, characterized in that: The guide shell (1101) is provided with through holes (1103) arranged at intervals to allow the heat-conducting medium in the ice bath shell (2) to pass through.

8. The detection and analysis device for immune protein according to claim 5, characterized in that: The rotating assembly comprises: A fixing member (1201) is fixedly connected to the placement frame (6), and the limiting plate (1006) is a part of the spherical shell; A connecting frame (1202), rotatably connected to the fixing member (1201); A movable shell (1203) is fixedly connected to the connecting frame (1202), the movable shell (1203) is slidably connected to the telescopic end of the hydraulic push rod (5), and a second elastic element (1204) is fixedly connected between the fixing member (1201) and the movable shell (1203); A limiting ring (1205) is fixedly connected to the telescopic end of the hydraulic push rod (5), and the limiting ring (1205) is spline-connected to the moving shell (1203).

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