High-throughput living cell imager LED array light source light following system and control method

Abstract

The invention discloses a high-throughput living cell imager LED array light source light following system and a control method, and relates to the technical field of instrument design and control, and the system is characterized in that an objective table and an LED light source are relatively fixed, an objective lens moves relative to the objective table, the movement stroke of the objective lens is matched with the LED light source, and the light source light following system is in signal connection with the objective lens and the LED light source. The control module is used for controlling the LED light source corresponding to the real-time position of the objective lens to be turned on or off. According to the invention, the problem of cell phototoxicity is solved only when the light source is lightened when the light following system controls imaging, the problem of mismatching of the light and the objective lens is solved through the LED array, the circuit implementation principle of the array LED is utilized, and the functions of light adjustability, constant current, RS485 control and the like are added, so that the light brightness is more stable than that of the existing LED array, and the system is suitable for large-scale popularization and application. The LED lamp does not change along with factors such as external voltage and temperature, has a more stable light supplementing effect, and achieves the purpose of controlling each lamp bead to be stably lightened at any brightness.

Description

Technical Field

[0001] The present invention relates to the technical field of instrument design and control, and more particularly to a high-throughput living cell imaging instrument LED array light source light following system and control method. Background Art

[0002] Live cell imaging detection technology is a commonly used technology in the fields of biology and biomedicine, which is used to observe and record the structure, function and interaction of cells at the level of living cells. This technology can obtain images and data in real time in the state of living cells, providing a powerful tool for studying cell biology, cell signaling, drug development and other fields. At present, traditional live cell imaging detection technologies include morphological observation equipment such as fluorescence microscopes, confocal microscopes, and two-photon microscopes, but these microscopic imaging techniques cannot observe cells dynamically for a long time. The high-throughput live cell imagers developed on this basis are increasingly favored by scientific researchers. This type of instrument can be placed in a cell culture incubator, which can maintain the biological activity of cell samples for a long time, making it possible to observe the dynamic activities of cells for a long time.

[0003] However, the high-throughput live-cell imaging industry is still in its initial stage, and high-throughput live-cell imaging generally has automatically controlled XYZ axis motion components, which can perform microscopic imaging and long-term observation of high-throughput specimens. Among them, there are two types of movement mechanisms for the stage of high-throughput live-cell imaging. One is that the objective lens is stationary, and the movement of the stage drives the living cell specimen on it to move to the top of the objective lens and image it. The other is that the stage and the living cell specimen on it are stationary, and the objective lens and imaging module are integrated on a mobile platform. The movement of the mobile platform moves the objective lens and imaging module to the bottom of the specimen area to be observed for imaging. Obviously, for living cell specimens that are not suitable for movement or vibration, the method of keeping the specimen stationary and moving the objective lens is the best solution, but this solution is extremely complex from design to control for the light source required for microscopic imaging. First, because light is phototoxic to living cells, long-term illumination will affect the normal physiological state of living cells and even cause the death of living cells; secondly, for high-throughput (large number of samples) imaging and detection, each specimen must be illuminated by a light source, otherwise the image will be unclear or even impossible to image.

[0004] Therefore, how to propose a high-throughput living cell imager LED array light source light following system and control method to solve the problems of light source fill light and cell phototoxicity when the light following system controls imaging, and improve the stability of light brightness and fill light effect are issues that technical personnel in this field urgently need to solve. Summary of the invention

[0005] In view of this, the present invention provides a high-throughput live cell imaging instrument LED array light source light following system and control method, which utilizes the circuit realization principle of array LED, and adds functions such as adjustable light, constant current, and RS485 control on this basis. The light brightness is more stable than the current LED array, and will not change with external voltage, temperature and other factors, and has a more stable fill light effect. It can also conveniently control each lamp bead to light up stably at any brightness. In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A high-throughput living cell imager LED array light source light following system comprises: an objective lens, an object stage, an LED light source and a light source light following system, wherein the object stage and the LED light source are relatively fixed, the objective lens moves relative to the object stage, the movement stroke of the objective lens is adapted to the LED light source, and the light source light following system is respectively connected to the objective lens and the LED light source signal, and is used to control the LED light source corresponding to the real-time position of the objective lens to light up or turn off.

[0007] Optionally, a high-throughput living cell specimen is placed on the stage.

[0008] Optionally, the light source light following system includes: a main control board, an RS485 conversion circuit, a single-chip microcomputer circuit, an indicator light circuit and an LED constant current drive dimming circuit;

[0009] The main control board is connected to the single-chip circuit signal through the RS485 conversion circuit, and the single-chip circuit is respectively connected to the indicator light circuit, the LED light source and the LED constant current drive dimming circuit signal.

[0010] Optionally, it also includes: a serial port to parallel port circuit, which is signal-connected to the single-chip microcomputer circuit and is used to expand the IO port of the single-chip microcomputer circuit.

[0011] Optionally, the LED light source is an LED array circuit.

[0012] Optionally, a power supply circuit is also included, which includes a DC-DC converter for converting 12V into 5V and 3.3V to provide power for the single-chip microcomputer circuit, main control board or LED light source.

[0013] Optionally, the LED array includes H lamp beads, arranged in M ​​columns and N clusters, M*N=H, each column is connected in a common cathode manner, and each cluster is connected in a common anode manner. If the lights in a certain column and a certain cluster are lit, the single-chip microcomputer circuit controls the conduction of the signals corresponding to the IO and PWM outputs of the LED array respectively, and controls the lamp beads from the darkest to the brightest through the PWM signal with a duty cycle of 0 to 100%.

[0014] Optionally, the indicator light circuit uses green, blue, yellow, red and white lights to connect to the IO of the single-chip computer in alternating yin and yang; if the green light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a low level; if the blue light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a high level; if the yellow light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a low level; if the red light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a high level; if the white light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a low level, and LD3 outputs a high level.

[0015] Optionally, the indicator light circuit also includes a breathing light mode, which outputs a PWM signal through PWM software with a timer interrupt as the timing, and cyclically changes the PWM signal duty cycle from low to high and then from high to low.

[0016] Optionally, a method for controlling a light following system of an LED array light source of a high-throughput living cell imaging instrument comprises:

[0017] Store the position relationship of the LED light source into the main control board;

[0018] The position of the corresponding LED light source is obtained according to the real-time position of the objective lens;

[0019] The main control board sends the position of the LED light source to the single-chip microcomputer circuit through the RS485 conversion circuit;

[0020] The single chip microcomputer circuit drives the LED light source according to the position of the LED light source and the LED constant current driving dimming circuit, controls the LED light source corresponding to the real-time position of the objective lens to light up or turn off, and controls its brightness.

[0021] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a high-throughput living cell imager LED array light source light following system and control method, which has the following beneficial effects:

[0022] The present invention proposes a high-throughput living cell imaging instrument LED array light source light following system, including: an objective lens, an objective stage, an LED light source and a light source light following system, wherein the objective stage and the LED light source are relatively fixed, the objective lens moves relative to the objective stage, the objective lens movement stroke is adapted to the LED light source, and the light source light following system is respectively connected to the objective lens and the LED light source signal, and is used to control the LED light source corresponding to the real-time position of the objective lens to light up or turn off. The present invention solves the problem of cell phototoxicity by controlling the light source to light up during imaging through the light following system, and solves the problem of mismatch between light and objective lens (the light source is not directly above the objective lens after the objective lens moves) through the LED array, ensuring that living cells remain healthy and active during the entire experiment. At the same time, this design also brings the following advantages: 1. Reduce mechanical vibration: the specimen does not move, which means that the movement of mechanical parts is reduced, and the light follows the lens to control the lighting of the lamp beads at the corresponding position, which significantly reduces the impact of mechanical vibration on imaging quality. 2. Stability and consistency: The fixed specimen position can ensure that the position of the specimen relative to the detector and the light source is consistent each time imaging is performed, which helps to improve the consistency and repeatability of imaging. 3. Simplified system design: The solution of moving the objective lens without moving the specimen and the light source simplifies the system design and reduces the need for complex mechanical structures and drive devices. This not only reduces the manufacturing cost of the equipment, but also may improve the reliability and life of the equipment. 4. High-precision imaging: Moving the objective lens can control the focal plane more accurately, thereby achieving high-precision imaging. The movement of the objective lens can be precisely controlled at the micron level, which is very important for capturing subtle changes in the internal structure of cells. 5. Flexibility: Due to the design and control of the LED array, the objective lens can be moved freely, and the user can adjust the size and position of the imaging area as needed. This flexibility enables the device to adapt to specimens of different types and sizes, increasing the versatility of the device. 6. Low thermal effect: Compared with traditional light sources, LED array light sources generate less heat, which is very important for imaging living cells. Excessive heat may damage cells and affect the authenticity of experimental results. The low thermal effect of LED array light sources helps maintain the physiological state of cells. 7. Energy saving and environmental protection: LED array light sources have high-efficiency energy conversion characteristics and consume less energy than traditional light sources (such as halogen lamps). This not only reduces operating costs, but also helps protect the environment. 8. High flexibility and adaptability: The design of the mobile objective lens and the light source of the LED array allows the system to easily adapt to different types of well plates and specimen sizes. Whether it is a multi-well plate, a single-well plate, or other containers of special shapes, the objective lens and the light source of the LED array can be adjusted as needed to ensure the best imaging effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0024] Figure 1 The schematic diagram of the single chip microcomputer circuit provided by the present invention.

[0025] Figure 2 This is a schematic diagram of the indicator light circuit provided by the present invention.

[0026] Figure 3 This is a schematic diagram of the LED array circuit provided by the present invention.

[0027] Figure 4 This is a schematic diagram of the LED constant current drive dimming circuit provided by the present invention.

[0028] Figure 5 The schematic diagram of the serial port to parallel port circuit provided by the present invention.

[0029] Figure 6 The RS485 conversion circuit schematic diagram provided by the present invention.

[0030] Figure 7 This is a schematic diagram of the power supply circuit principle provided by the present invention.

[0031] Figure 8 A schematic flow chart of a method for controlling a light following system of an LED array light source for a high-throughput living cell imager provided by the present invention. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] An embodiment of the present invention discloses a high-throughput living cell imager LED array light source light following system, comprising: an objective lens, a stage, an LED light source and a light source light following system, wherein the stage and the LED light source are relatively fixed, the objective lens moves relative to the stage, the movement stroke of the objective lens is adapted to the LED light source, and the light source light following system is respectively connected to the objective lens and the LED light source signal, and is used to control the LED light source corresponding to the real-time position of the objective lens to light up or turn off.

[0034] Furthermore, a high-throughput living cell specimen is placed on the stage.

[0035] Furthermore, the light source light following system includes: a main control board, an RS485 conversion circuit, a single chip microcomputer circuit, an indicator light circuit and an LED constant current drive dimming circuit;

[0036] The main control board is connected to the single-chip circuit signal through the RS485 conversion circuit, and the single-chip circuit is respectively connected to the indicator light circuit, the LED light source and the LED constant current drive dimming circuit signal.

[0037] Furthermore, it also includes: a serial port to parallel port circuit, which is connected to the single-chip circuit signal and is used to expand the IO port of the single-chip circuit.

[0038] Furthermore, the LED light source is an LED array circuit.

[0039] Furthermore, it also includes a power supply circuit, which includes a DC-DC converter for converting 12V into 5V and 3.3V to provide power for the single-chip microcomputer circuit, the main control board or the LED light source.

[0040] Furthermore, the LED array includes H lamp beads, which are arranged in M ​​columns and N clusters, M*N=H, each column is connected in a common cathode manner, and each cluster is connected in a common anode manner. If the lamps in a certain column and a certain cluster are lit, the single-chip microcomputer circuit controls the conduction of the signals corresponding to the IO and PWM outputs of the LED array respectively, and controls the lamp beads from the darkest to the brightest through the PWM signal with a duty cycle of 0 to 100%.

[0041] Furthermore, the indicator light circuit uses green, blue, yellow, red and white lights to connect to the IO of the single-chip computer in alternating yin and yang; if the green light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a low level; if the blue light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a high level; if the yellow light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a low level; if the red light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a high level; if the white light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a low level, and LD3 outputs a high level.

[0042] Furthermore, the indicator light circuit also includes a breathing light mode, which outputs a PWM signal through PWM software with a timer interrupt as the timing, and cyclically changes the PWM signal duty cycle from low to high and then from high to low.

[0043] In a specific embodiment, a method for controlling a light following system of an LED array light source of a high-throughput living cell imaging instrument is provided. Figure 8 As shown, including:

[0044] Store the position relationship of the LED light source into the main control board;

[0045] The position of the corresponding LED light source is obtained according to the real-time position of the objective lens;

[0046] The main control board sends the position of the LED light source to the single-chip microcomputer circuit through the RS485 conversion circuit;

[0047] The single chip microcomputer circuit drives the LED light source according to the position of the LED light source and the LED constant current driving dimming circuit, controls the LED light source corresponding to the real-time position of the objective lens to light up or turn off, and controls its brightness.

[0048] In a specific implementation, since the main purpose of the live cell imager is to perform long-term imaging of special specimens such as living cells, and for high-throughput living cell specimens, the method of not moving the specimen with the objective lens is the best solution. Therefore:

[0049] 1) In order not to interfere with the normal activity of cells, the light source cannot irradiate the cells for a long time, that is, the control system controls the light source to light up synchronously only when the imaging system needs to image or take pictures, and it is not lit at other times;

[0050] 2) Since the objective lens moves between different cell samples, the best solution is to place the light source directly above the objective lens. If there is a single light source, in order to keep the light source directly above the objective lens, the light source and the objective lens must be synchronized. On the one hand, this linkage requires the light source to have a displacement and control system. On the other hand, since there is a large area of ​​living cell samples in between, the simultaneous movement of the light source and the objective lens will cause errors, making it difficult to implement. In addition, the vibration caused by multiple sets of moving parts will also interfere with the living cell samples.

[0051] Therefore, the present invention proposes a solution: using multiple LEDs to form a large-area LED array to replace the movement of the light source. The LED array light source can provide a highly uniform light intensity distribution, which is particularly important for imaging living cells. Cells need to be observed under stable lighting conditions to avoid phototoxicity or photobleaching effects; and through precise software calculation and control system, no matter where the objective lens moves to, the LED light source directly above the objective lens position is lit, and other light sources in the LED array are not lit. In this way, the LED array light source does not need to move, which solves the problem of asynchronous movement of the objective lens and the single light source, that is, solves the problem of cell phototoxicity, and solves the problem of mismatch between light and objective lens (the light source is not directly above the objective lens).

[0052] Specifically, as a light-filling component of a microscopic imaging system, it mainly solves the problems of light-filling and cell phototoxicity when the light-following system controls imaging. Cells need light-filling during microscopic imaging, but long-term exposure to light will affect cell survival and lifespan. By controlling the intensity of the light and the lighting time, the dose and intensity of light irradiation to the cells can be controlled, effectively controlling the effect of light on the cells during the culture process. The problem of mismatch between light and objective lens (the light source is not directly above the objective lens after the objective lens moves) is solved by the LED array. When the microscope is moving, the light needs to be accurately controlled to light up at the appropriate intensity above. The LED lamp beads in the array are arranged at a spacing of 4.5mm to cover the entire moving area.

[0053] The present invention utilizes the circuit realization principle of array LED, and adds functions such as adjustable light, constant current, RS485 control, etc. on this basis. The light brightness is more stable than that of the current LED array, and will not change with external voltage, temperature and other factors, and has a more stable microscopic light source fill light effect. It also has the function of conveniently controlling each lamp bead to light up stably at any brightness.

[0054] In a specific embodiment, a method for controlling a light following system of an LED array light source of a high-throughput living cell imaging instrument comprises the following steps: using a HC32F460 single-chip microcomputer as the core of the component, such as Figure 1 As shown, it receives the lighting control instructions issued by the main control board, executes them accurately, and returns information; Figure 7 As shown, the power circuit stabilizes the 12V power supply to 5V and 3.3V to supply power to the microcontroller and chip; Figure 6 As shown, it is connected to the main control board through the RS485 conversion circuit; Figure 3 As shown, the array LED fill light circuit plays a role in fill light; Figure 4 As shown, the constant current circuit is used to keep the current of the LED constant, so that the light intensity remains constant; the single-chip microcomputer circuit is used to control the lighting of the LED and output PWM signals to keep the light intensity constant; Figure 5 As shown in the figure, the IO of the microcontroller can be expanded through the serial port to parallel port circuit; Figure 2 As shown, the indicator light circuit is used to display the indication status. Through the indicator light circuit, the 5-way indicator light adopts a 4-way IO ingenious circuit design, and the number of IOs occupied is reduced. This embodiment uses the above circuit design to reduce component costs, the circuit is simple and easy to maintain and produce, and the RS485 communication has strong anti-interference performance and high communication reliability.

[0055] In a specific implementation, a single-chip microcomputer is used as the core to control the anode conduction of the LED through IO and extended IO, and then outputs the PWM signal to the LED constant current chip to achieve constant current dimming; the LED array uses 384 lamp beads, arranged in 16 columns and 24 clusters, each column is connected in a common cathode manner, and each cluster is connected in a common anode manner. If the lights in a certain column and a certain cluster need to be lit, the single-chip microcomputer controls the IO of the LED array and the corresponding signals of the PWM output to be turned on; the PWM signal is output with a duty cycle of 0 to 100% to control the lights from the darkest to the brightest.

[0056] In a specific implementation, the indicator light circuit uses green, blue, yellow, red, and white LED lights and is connected to the IO of the single-chip computer in alternating yin and yang. If the green light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a low level. If the blue light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a high level. If the yellow light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a low level. If the red light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a high level. If the white light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a low level, and LD3 outputs a high level.

[0057] In a specific implementation, the indicator light can also be controlled to operate in a breathing light mode, which is implemented using software PWM. The PWM signal output is realized using a timer interrupt as the timing, and then the PWM signal duty cycle is continuously changed from low to high and then from high to low to achieve a breathing light effect.

[0058] In a specific implementation, the light following effect is achieved, and the main control of the living cell imager sends a signal through the serial port to RS485 to control the LED fill light corresponding to the real-time position of the camera to light up or turn off, and control its brightness. The position relationship of the light is stored in the main control of the living cell imager in advance, and the lighting method of the indicator light is the same.

[0059] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0060] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A high-throughput living cell imaging instrument LED array light source light following system, characterized in that: include: An objective lens, an object stage, an LED light source and a light source light following system, wherein the object stage and the LED light source are relatively fixed, the objective lens moves relative to the object stage, the object lens movement stroke is adapted to the LED light source, and the light source light following system is respectively connected to the objective lens and the LED light source signal, and is used to control the LED light source corresponding to the real-time position of the objective lens to light up or turn off.

2. The high-throughput living cell imaging device LED array light source light following system according to claim 1, characterized in that: A high-throughput living cell specimen is placed on the stage.

3. The high-throughput living cell imaging device LED array light source light following system according to claim 1, characterized in that: The light source light following system comprises: a main control board, an RS485 conversion circuit, a single chip microcomputer circuit, an indicator light circuit and an LED constant current drive dimming circuit; The main control board is connected to the single-chip circuit signal through the RS485 conversion circuit, and the single-chip circuit is respectively connected to the indicator light circuit, the LED light source and the LED constant current drive dimming circuit signal.

4. The high-throughput living cell imaging device LED array light source light following system according to claim 3, characterized in that: Also includes: A serial port to parallel port circuit is connected to the single chip circuit signal and is used to expand the IO port of the single chip circuit.

5. The high-throughput living cell imaging device LED array light source light following system according to claim 1, characterized in that: The LED light source is an LED array circuit.

6. The high-throughput living cell imaging device LED array light source light following system according to claim 1, characterized in that: It also includes a power supply circuit, which includes a DC-DC converter for converting 12V into 5V and 3.3V to provide power for the single-chip microcomputer circuit, the main control board or the LED light source.

7. The high-throughput living cell imaging device LED array light source light following system according to claim 5, characterized in that: The LED array includes H lamp beads, which are arranged in M ​​columns and N clusters, M*N=H, each column is connected in a common cathode manner, and each cluster is connected in a common anode manner. If the lamps in a certain column and a certain cluster are lit, the single-chip microcomputer circuit controls the IO and PWM output of the LED array respectively to turn on the signals corresponding to the output, and controls the lamp beads from the darkest to the brightest through the PWM signal with a duty cycle of 0 to 100%.

8. The high-throughput living cell imaging device LED array light source light following system according to claim 3, characterized in that: The indicator light circuit uses green, blue, yellow, red and white lights to connect to the IO of the single-chip computer in alternating yin and yang; if the green light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a low level; if the blue light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a high level; if the yellow light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a high level, and LD3 outputs a low level; if the red light needs to be controlled to light up, LD4 outputs a high level, LD1 outputs a high level, LD2 outputs a low level, and LD3 outputs a high level; if the white light needs to be controlled to light up, LD4 outputs a low level, LD1 outputs a low level, LD2 outputs a low level, and LD3 outputs a high level.

9. The high-throughput living cell imaging device LED array light source light following system according to claim 8, characterized in that: The indicator light circuit also includes a breathing light mode, which uses PWM software to output PWM signals with timer interrupts as the timing, and cyclically changes the PWM signal duty cycle from low to high and then from high to low.

10. A method for controlling a light following system of an LED array light source for a high-throughput living cell imaging instrument, characterized in that: include: Store the position relationship of the LED light source into the main control board; The position of the corresponding LED light source is obtained according to the real-time position of the objective lens; The main control board sends the position of the LED light source to the single-chip microcomputer circuit through the RS485 conversion circuit; The single chip microcomputer circuit drives the LED light source according to the position of the LED light source and the LED constant current driving dimming circuit, controls the LED light source corresponding to the real-time position of the objective lens to light up or turn off, and controls its brightness.

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