A method of promoting egg production in queen bees of apis mellifera by feeding juvenile hormone

Abstract

The application provides a method for promoting oviposition of queen bees of Apis mellifera by feeding the queen bees with juvenile hormone, wherein the queen bees are quantitatively fed with a 500ug / ml JHIII sugar solution, and each queen bee is fed with 20ul, so that the effective mass of JHIII fed to each queen bee is 10ug. The results show that the number and quality of eggs laid by the queen bees are significantly improved, and the queen bees can still maintain a good physiological state.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bee breeding, and particularly relates to a method for promoting oviposition of Apis mellifera queen bees by feeding juvenile hormone. BACKGROUND

[0002] In traditional bee breeding, the oviposition capacity of queen bees directly affects the reproductive efficiency of bee colonies and the economic benefits of bee breeding. Due to environmental changes, improper feeding management, queen bee health and other factors, the oviposition amount of queen bees often fluctuates. In order to improve the oviposition quantity and quality of queen bees, researchers have tried various methods, including optimizing feed formulations, adjusting environmental conditions, etc. However, the effects of these methods are limited, and often depend on long-term management of the environment and feed. Therefore, how to adjust the physiological state of queen bees to improve oviposition efficiency has become a problem to be solved in the field of bee breeding.

[0003] Juvenile hormone, as a hormone that promotes the growth and development of insects, has been applied in the breeding research of various insects. In bee breeding, appropriate juvenile hormone can promote the development of queen bee ovaries and improve their oviposition capacity. The existing technology has not fully utilized juvenile hormone to achieve high-efficiency oviposition of queen bees, so the present application provides a new technology for promoting oviposition of Apis mellifera queen bees by feeding juvenile hormone. SUMMARY

[0004] The purpose of the present application is to provide a method for promoting oviposition of Apis mellifera queen bees by feeding juvenile hormone. This method can significantly improve the oviposition efficiency of queen bees, increase the reproductive capacity of bee colonies, and thus improve the production efficiency of bee breeding.

[0005] To achieve the above-mentioned purpose, the present application adopts the following technical solutions.

[0006] A method for promoting oviposition of Apis mellifera queen bees by feeding juvenile hormone, which comprises adding juvenile hormone to the queen bee feed to prepare a feed solution and then feeding the queen bees.

[0007] The juvenile hormone is R-JH III or S-JH III.

[0008] The juvenile hormone is R-JH III.

[0009] The concentration of juvenile hormone in the feed solution is 500 μg / ml.

[0010] The feeding amount in step (2) is 20 μL.

[0011] The feeding operation is as follows: the queen bee is taken out of the beehive and stuck on a clean culture dish with double-sided tape, the prepared feed solution with juvenile hormone is fed to the queen bee with a pipette, and then the queen bee is put back into the original beehive after eating.

[0012] The feeding is starving the queen bee before feeding.

[0013] The method for promoting egg production of Apis mellifera queen bees by feeding juvenile hormone provided by the present application has the following advantages:

[0014] 1. Significantly improve egg production efficiency: By adjusting the hormone level in the queen bee's body, the egg production capacity of the queen bee can be significantly improved, thereby promoting the rapid reproduction of the bee colony.

[0015] 2. Improve production efficiency: Enhance the reproductive capacity of the bee colony and improve the honey production and reproduction efficiency of bee breeding.

[0016] 3. Reduce environmental dependence: Compared with traditional environmental regulation methods, the method of the present application relies more on the regulation of endogenous hormones, reducing the influence of external environment.

[0017] 4. Strong adaptability: The method is suitable for Apis mellifera breeding under different environmental conditions and has strong universality. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The figure is a marking method for the egg production area described in the present application.

[0019] Figure 2 The figure is a feeding schematic diagram.

[0020] Figure 3 The figure is a comparison of the egg production of queen bees fed with different feed. DETAILED DESCRIPTION

[0021] The present application will be further described in detail below in conjunction with the examples and drawings.

[0022] EXAMPLE

[0023] In an experimental bee colony, Apis mellifera queen bees were selected and operated according to the following steps: preparation of juvenile hormone solution: juvenile hormones R-JH III and S-JH III were selected and dissolved in 50% sugar water solution at a concentration of 500 μg / ml. The bee colony was divided into 4 groups for feeding, wherein the CK group was fed with 50% sugar water solution; the SCK group was fed with 50% sugar water solution containing 2% acetonitrile; the R-JH III group was fed with 50% sugar water solution containing 500 μg / ml R-JH III; and the S-JH III group was fed with 50% sugar water solution containing 500 μg / ml S-JH III.

[0024] Marking the beehives: 12 boxes of bees were prepared and marked, No. 1 box, No. 2 box... No. 12 box, each box of bees placed 4 spleens, marked each spleen, for example: No. 1 box: 1-1, 1-2, 1-3... Finally, the amount of eggs produced by each spleen was added up as the total number of eggs produced by the beehive. The control group CK was No. 1-3 box, the solvent control group SCK was No. 4-6 box, R-JH III was No. 7-9 box, and S-JH III was No. 10-12 box.

[0025] Feeding the queen bee: The queen bee was taken out and recorded which No. box the queen bee came from, then put back into the original beehive after feeding the medicine. The queen bee was starved after being taken out, then fixed on a clean culture dish with double-sided tape, and then slowly fed 20 μl of feed solution to the queen bee with a pipette. After the queen bee finished eating the feed solution, it was put back into the original beehive.

[0026] Feeding frequency: The queen bee was fed with juvenile hormone sugar solution only once in this experiment.

[0027] Regularly record the number of eggs: The egg stage of Italian bees is 3 days, and the larvae develop on the 4th day. The egg-laying area of the queen bee was marked on the same day after feeding the medicine (without counting the number of eggs). On the 4th day after feeding the medicine, the newly laid egg-laying area of the queen bee was marked, and compared with the previous area (at this time, the eggs in the previous area have reached the larva stage and developed into larvae, so the number of eggs counted on the 4th day is the newly laid eggs after feeding the medicine, which is the first formal count). The number of eggs laid by the queen bee in 3 days can be counted, and the number of eggs is recorded regularly, with a frequency of once every 3 days.

[0028] The experiment was divided into 4 times, and each beehive was placed in the same position to ensure that each beehive was tested in the same environment. The second test was conducted after the first test, and so on. Each test interval was 3 days.

[0029] Health monitoring: The health status of the queen bee was checked every 3 days to ensure that the queen bee did not have health problems caused by excessive hormones; the quality of the eggs was checked every 3 days to observe whether the newly laid eggs could develop normally into larvae.

[0030] Statistical table of the number of eggs laid by Italian queen bees after feeding

[0031] Unit: grains

[0032]

[0033] The experimental results show that the solvent control group SCK has no significant difference in egg production compared with the control group CK, and the solvent has no effect on the egg production of the queen bee. By feeding the Italian queen bee with juvenile hormone, the egg production of the queen bee is significantly improved compared with the control group, and the health status of the queen bee is good, and there is no obvious side effect. The quality of the newly produced eggs is good, full and shiny, and the newly produced eggs can develop into healthy larvae normally. The results of 4 experiments show that the total egg production of R-JH III feeding is 1.4, 1.2, 0.7 and 1.0 times higher than that of the CK control group respectively; the total egg production of S-JH III feeding is 1.4, 0.9, 0.1 and 0.2 times higher than that of the CK control group respectively. The total egg production of the 4 investigations shows that the total egg production of CK is 7301, the total egg production of SCK is 7848, the total egg production of R-JH III is as high as 14822, and the total egg production of S-JH III is 11340. The total egg production of R-JH III is 2 times of CK, and the total egg production of S-JH III is 1.6 times of CK. It can be seen that JH III can significantly promote the egg production of the queen bee, and R has higher efficiency.

[0034] The results of the third investigation show that the egg production of R-JH III and S-JH III is smaller than that of the other three investigations, and the growth is not obvious. The reason is that we only put 4 bee guts in each experimental beehive for egg production. Since the egg production of the queen bee is huge in the first and second investigations, the number of available holes for the queen bee to lay eggs is small in the third investigation, which leads to the increase of the egg production of the queen bee. However, in the fourth investigation, R-JH III shows a sharp increase, which also shows that it has long-term effect on promoting egg production.

[0035] The application provides a method for promoting the egg production of Italian queen bees by feeding juvenile hormone to the queen bees. The queen bees are starved and fixed by double-sided tape after being taken out, and are accurately fed by using a pipette. Specific juvenile hormone JH III is fed, and the specific mass of the juvenile hormone is fed. 500 μg / ml of JH III sugar solution is fed, and 20 μl of the solution is fed to each queen bee, so that the effective mass of JH III fed to each queen bee is 10 μg. The feeding is performed once every 3 days.

[0036] The method can significantly improve the egg production and quality of the queen bees, promote the reproduction of the bee colony, and improve the production efficiency of the bee breeding. The method can effectively solve the problem of insufficient egg production of the queen bees in traditional bee breeding, and provides a new technical means for the bee breeding industry.

Claims

1. A method of promoting egg production in queen bees of Apis mellifera by feeding them with juvenile hormone, characterized in that, The method comprises the following steps: (1) preparing a feed solution: adding juvenile hormone into queen bee feed to prepare a feed solution; (2) feeding queen bees: feeding Italian queen bees; The juvenile hormone is R-JH III; The concentration of R-JH III in the feed solution is 500 μg / mL; The volume of the feed solution for each queen bee at each time is 20 μL.

2. The method of claim 1 for promoting egg production in queen bees of Apis mellifera by feeding them with juvenile hormone, characterized in that, The feeding is specifically performed as follows: after the queen bee is taken out from a beehive, the queen bee is adhered to a clean culture dish by double-sided adhesive tape, the prepared feed solution with juvenile hormone is fed to the queen bee by a pipette, and the queen bee is put back to the beehive after the queen bee finishes eating.

3. The method of claim 2 for promoting egg production in queen bees of Apis mellifera by feeding them with juvenile hormone, characterized in that, The feeding is performed after the queen bee is starved.

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