Cryogenic vial protection cover and cryogenic vial

Abstract

The utility model relates to the field of experimental consumables, in particular to a cryopreservation tube protective cover and a cryopreservation tube. The cryopreservation tube protection cover is matched with a tube body and a cover body of a cryopreservation tube and comprises a shell and a separation membrane. The housing has a first section and a second section opposite the first section. The first section of the shell is matched with the pipe body and connected with the pipe body in a sealed mode. And the second section of the shell is matched with the cover body and is detachably connected with the cover body. The edge of the separation film is connected to the inner wall of the shell and used for closing the opening of the pipe body. The cryopreservation tube comprises the tube body, a cover body and a protective cover. Whether the cryopreservation tube is opened for the first time or not is judged by judging whether the separation membrane is punctured and damaged or not, and the uniqueness function of a biological sample can be achieved easily. A sample is protected by the separation membrane after the cover body is opened and before sampling, so that the cleanliness of the sample is improved.

Description

Technical Field

[0001] This utility model relates to the field of laboratory consumables, and in particular to a cryopreservation tube protective cap and a cryopreservation tube. Background Technology

[0002] Cryopreservation tubes, also known as cryovials, cell cryopreservation tubes, etc., are mainly used for the transfer, cryogenic transport, and storage of biological samples such as cells, tissues, DNA, and RNA. They are commonly used in biological research and medical fields. Cryopreservation tubes are frequently used as containers and carriers when preserving and transferring biological samples.

[0003] Due to the special nature of biological samples, only one sampling is usually permitted. Samples obtained by sampling twice from the same cryovial are generally considered contaminated. Therefore, in biological experiments, samples in cryovials are discarded after the first sampling. However, in practice, in situations such as hospitals where large quantities of samples need to be preserved and processed efficiently, sample processing is often delayed. This can lead to confusion between samples that have already been sampled and those that haven't, resulting in contaminated samples that hinder the experiment. Utility Model Content

[0004] The purpose of this invention is to provide a cryopreservation tube protective cap and cryopreservation tube that can distinguish whether a sample has been collected once.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A cryopreservation tube protective cap, which is fitted to the tube body and cap body of the cryopreservation tube, includes:

[0007] The housing has a first section and a second section connected to the first section, the first section of the housing is fitted to the tube and is sealed to the tube, and the second section of the housing is fitted to the cover and is detachably connected to the cover;

[0008] A separator membrane, the edges of which are connected to the inner wall of the housing, is used to close the opening of the tube.

[0009] Optionally, the protective cover is constructed in a one-piece molding manner.

[0010] Optionally, the separator membrane is provided with an opening point, and the side of the separator membrane away from the tube body is formed with a plurality of thinning grooves passing through the opening point.

[0011] Optionally, the thickness at the opening point is less than or equal to 0.2 mm.

[0012] Optionally, the protective cover further includes an abutment portion, one end of which is connected to the inner wall of the housing away from the tube body, and the abutment portion and the first section of the housing form a sealing groove facing the opening of the tube body, the opening of the tube body being embedded in the sealing groove and abutting against the inner wall of the sealing groove.

[0013] Secondly, this utility model also provides a cryopreservation tube, including the aforementioned protective cap, tube body and cap body.

[0014] Optionally, a buckle is provided on the outer wall of the tube, and a blocking part that cooperates with the buckle is provided on the inner wall of the first section of the housing. When the first section of the housing is sleeved and connected to the opening of the tube, the buckle is constrained by the blocking part.

[0015] Optionally, the opening of the tube is connected to the first section of the housing via a first thread. The buckle has an inclined surface at one end away from the cover that slopes toward the outside of the tube. A blocking portion is formed on the inner wall of the housing near one end of the tube, with a position corresponding to the buckle. When the first section of the housing is connected to the opening of the tube, the buckle abuts against the blocking portion and is located between the blocking portion and the thread.

[0016] Optionally, the cover includes a mating part, a sealing part, and a closing part. The mating part is mated to the second section of the housing and is detachably connected to the second section of the housing. The sealing part is connected to the end of the mating part away from the tube and is sleeved on the inner side of the mating part. The outer wall of the sealing part abuts against the inner wall of the second section of the housing. The edge of the closing part is connected to the sealing part and is used to close the end of the mating part away from the tube.

[0017] Optionally, the closure portion is recessed toward the side closer to the tube body.

[0018] According to a first aspect of this invention, after the collected biological sample is placed inside the cryovial, the first section of the protective cap is installed onto the tube body. At this point, the septum membrane of the protective cap covers the opening of the tube body, closing the opening. The cap is then installed onto the second section of the shell to protect the septum membrane. During sampling, the cap is opened, and the septum membrane is punctured and broken using a sampler to extract the sample from inside the tube. In case of sample confusion, the integrity of the septum membrane can be used to determine whether a sample has been sampled once, thus ensuring the uniqueness of the biological sample. Furthermore, when reagents need to be added to the biological sample inside the tube during sampling, this structure can distinguish whether reagent addition has been completed, preventing multiple additions of reagents to the tube. Additionally, after opening the cap, the inside of the tube remains sealed, helping to prevent the sample from being exposed to the external environment for extended periods.

[0019] Furthermore, constructing the protective cover from a single material through a one-piece molding process helps reduce production costs and improve production efficiency.

[0020] Furthermore, it is difficult to obtain a complete film when constructing products using one-piece molding techniques such as injection molding. By thickening the separator film as a whole and then thinning it locally using thinning grooves, it is helpful to form openings on the separator film that are thinner and easier to penetrate. Forming openings using multiple thinning grooves intersecting at the same point helps to increase the area of ​​the openings and reduce the difficulty of penetration.

[0021] Furthermore, by constraining the thickness of the opening point, it is ensured that the opening point of the separator membrane can be penetrated by the sampler, thereby ensuring the convenience of sampling.

[0022] Furthermore, by setting an abutment to form a sealing groove, the opening of the tube is completely embedded inside the protective cap, and a seal is achieved through mechanical compression, reducing the risk of sample contamination inside the tube.

[0023] According to a second aspect of this invention, the cryopreservation tube includes a protective cap, a tube body, and a cap body that cooperate with each other. The protective cap protects the contents of the tube body and indicates whether the tube body has been opened for the first time, which helps to reduce the risk of secondary sampling. Designing the protective cap, tube body, and cap body as a whole helps to improve the tightness of the fit and enhance the sealing performance.

[0024] Furthermore, by setting up snap-fit ​​and blocking parts, the protective cover is difficult to remove after being installed on the tube body, which helps to improve the reliability of the judgment on whether a sample has been taken.

[0025] Furthermore, by incorporating mating and sealing components, the sealing performance of the connection between the cover and the protective cover is improved, which helps to enhance the cleanliness of sample preservation.

[0026] Furthermore, by making the closure recessed towards the protective cap, the space enclosed by the septum membrane and the inside of the cap is reduced, which helps to reduce the contamination of the septum membrane by air inside the cap, thereby improving the cleanliness of sample preservation.

[0027] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure of the cryopreservation tube shown in Embodiment 1 of this utility model;

[0029] Figure 2 This is a cross-sectional view of the cryopreservation tube shown in Embodiment 1 of this utility model;

[0030] Figure 3 This is a schematic diagram of the structure of the protective cover shown in Embodiment 1 of this utility model.

[0031] Legend: 1-pipe body, 11-pipe opening, 111-first thread, 112-clamp, 12-limiting part, 2-cover, 21-fitting part, 22-sealing part, 23-closing part, 3-protective cover, 31-shell, 311-first section, 312-second section, 313-blocking part, 32-separating membrane, 321-thinning groove, 322-opening point, 33-contacting part, 331-sealing groove. Detailed Implementation

[0032] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0033] Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0034] Please see Figure 1 and Figure 2The cryopreservation tube protective cap 3, protected by this utility model application, is fitted to the tube body 1 and cap body 2 of the cryopreservation tube, and includes a shell 31 and a separator 32. The shell 31 has a first section 311 and a second section 312 connected to the first section 311. The first section 311 of the shell 31 fits into the tube body 1 and is sealed to the tube body 1. The second section 312 of the shell 31 fits into the cap body 2 and is detachably connected to the cap body 2. The edge of the separator 32 is connected to the inner wall of the shell 31 to close the opening of the tube body 1.

[0035] After the collected biological samples are placed inside the cryovial body 1, the first section 311 of the protective cap 3's shell 31 is installed onto the body 1. At this time, the septum 32 of the protective cap 3 covers the opening of the body 1, closing the opening. The cap 2 is then installed onto the second section 312 of the shell 31, thus protecting the septum 32. During sampling, the cap 2 is opened, and the septum 32 is punctured and broken using a sampler to extract the sample from inside the body 1. When sample confusion occurs, the integrity of the septum 32 can be used to determine whether a sample has been sampled once, thus ensuring the uniqueness of the biological sample. In addition, when reagents need to be added to the biological sample inside the body 1 during sampling, this structure can also distinguish whether reagent addition has been completed, preventing multiple additions of reagents to the body 1. Furthermore, after the cap 2 is opened, the body 1 remains sealed, helping to prevent the sample from being exposed to the external environment for extended periods.

[0036] Please refer to the following examples for details.

[0037] Example 1:

[0038] Please see Figure 1 The cryopreservation tube shown in a preferred embodiment of this application includes a tube body 1, a cap body 2, and a protective cap 3. The two ends of the protective cap 3 are respectively connected to the tube body 1 and the cap body 2.

[0039] Please see Figure 1 and Figure 2 The tube body 1 is a cylindrical tubular structure with an open top, the top of which is the tube body 11. The outer wall diameter of the tube body 11 is small, and a first thread 111 is formed on the outer wall. Below the first thread 111, a ring-shaped buckle 112 is formed. The cross-section of the buckle 112 is trapezoidal, and its inclined surface extends outward from the tube body 1 and away from the opening of the tube body 1. The lower outer wall of the tube body 11 expands outward to form a limiting part 12 with a larger diameter.

[0040] Please see Figure 1 , Figure 2 and Figure 3The protective cover 3 includes a housing 31, an abutment portion 33, and a separator 32, all integrally molded by injection molding. The housing 31 has a first segment 311 and a second segment 312 connected to the first segment 311. The first segment 311 of the housing 31 is configured as an annular shape to fit the tube body 11, and its inner wall is provided with another set of threads that fit the first thread 111. The first segment 311 of the housing 31 is screwed onto the outside of the tube body 11. An annular blocking portion 313 is formed on the inner wall of the first segment 311 of the housing 31 at a position corresponding to the buckle 112. The cross-sectional structure of the blocking portion 313 is a right angle with the notch facing the housing 31, facilitating the buckle 112 to reach below the blocking portion 313. With the side of the first segment 311 away from the second segment 312 as the bottom surface of the housing 31, when the first segment 311 of the housing 31 is connected to the tube 11, the bottom surface of the housing 31 abuts against the limiting part 12 of the tube 1, and the side of the buckle 112 near the bottom surface of the housing 31 abuts against the side of the blocking part 313 away from the bottom surface of the housing 31, thereby constraining the position of the protective cover 3 and fixing the position of the protective cover 3 relative to the tube 1. The end of the first segment 311 away from the bottom surface of the housing 31 tapers inward into a circle with a smaller diameter, and the edge extends in two directions parallel to the first segment 311 to form the second segment 312 and the abutting part 33. The second segment 312 extends in a direction away from the first segment 311. The abutting part 33 is fitted inside the first segment 311 and is shorter than the first segment 311. The diameter of the outer wall of the contact portion 33 matches the diameter of the outer wall of the tube body 11, so that the tube body 11 fits into the single-sided opening sealing groove 331 formed by the first section 311 and the contact portion 33, thereby achieving a seal on the tube body 11 through the contact and compression between the tube body 11 and the sealing groove 331. A separator membrane 32 is connected to the end of the contact portion 33 away from the second section 312. The edge of the circular separator membrane 32 is closed and connected to the contact portion 33, and the center of its circle near the side of the second section 312 is the opening point 322. Three sealing grooves 331 are formed on the surface of the separator membrane 32, passing through the opening point 322. The sealing grooves 331 are embedded inside the separator membrane 32, so that the thickness of the separator membrane 32 at the opening point 322 is only 0.1 mm, facilitating the sampler to penetrate the separator membrane 32. In this embodiment, the sealing grooves 331 are evenly distributed.

[0041] Please see Figure 1 and Figure 2The cover 2 includes a mating part 21, a sealing part 22, and a closing part 23. The inner diameter of the annular mating part 21 matches the outer diameter of the second section 312 of the housing 31, and it is detachably fitted onto the outside of the second section 312 of the housing 31 via a threaded structure. The end of the mating part 21 away from the tube 1 converges inward and extends toward the tube 1 in a direction parallel to the mating part 21, forming the sealing part 22. The sealing part 22 is fitted inside the mating part 21, and its outer wall diameter matches the inner wall diameter of the second section 312 of the housing 31, forming an annular groove structure with a bottom opening together with the mating part 21. When the cover 2 is connected to the protective cover 3, the inner wall of the cover 2 abuts against the outer wall of the sealing part 22, thereby ensuring the airtightness of the connection between the cover 2 and the protective cover 3. The edge of the closing part 23 is connected to the end of the sealing part 22 near the tube 1, so that the side of the cover 2 away from the tube 1 is closed. The middle part of the closing portion 23 is recessed towards the inside of the protective cover 3, forming a cylindrical groove concentric with the mating portion 21. The end of the groove away from the sealing portion 22 is conical, thereby reducing the internal space of the cover 2 and decreasing the contact between the separator membrane 32 and the ambient gas. The height of the mating portion 21 is slightly greater than the total height of the sealing portion 22 and the closing portion 23, ensuring that the inner wall of the closing portion 23 does not contact the plane when the cover 2 is placed on a flat surface, thereby reducing the risk of contamination.

[0042] The beneficial effects of this invention are as follows: The protective cap 3 indicates whether the sample inside the tube 1 has undergone sampling, helping to prevent sample confusion and multiple sampling of the same sample, thus ensuring sample cleanliness. The protective cap 3 also protects the sample inside the tube 1 after the cap 2 is opened, reducing the sample's exposure time to the environment. The protective cap 3 is integrally molded from a single material using injection molding, a simple and easy process. Furthermore, the structure of the tube 1 and cap 2 is only slightly different from existing products, requiring only minor adjustments to the mold, resulting in lower production costs.

[0043] Example 2:

[0044] The only difference between this embodiment and Embodiment 1 is that this embodiment only includes the protective cap 3, and the protective cap 3 in this embodiment does not include the blocking part 313. The protective cap 3 in this embodiment helps to reduce costs by adjusting its size to match the tube body 1 and cap body 2 of the existing cryopreservation tube.

[0045] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0046] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A protective cap for cryopreservation tubes, characterized in that, The tube body (1) and cap (2) that are used with cryopreservation tubes include: The housing (31) has a first section (311) and a second section (312) connected to the first section (311). The first section (311) of the housing (31) is fitted to the tube (1) and is sealed to the tube (1). The second section (312) of the housing (31) is fitted to the cover (2) and is detachably connected to the cover (2). A separator (32), the edge of which is connected to the inner wall of the housing (31), is used to close the opening of the tube (1).

2. The protective cover as described in claim 1, characterized in that, The protective cover (3) is constructed by integral molding.

3. The protective cover as described in claim 2, characterized in that, The separator (32) has an opening point (322), and the separator (32) has a plurality of thinning grooves (321) passing through the opening point (322) on the side away from the tube (1).

4. The protective cover as described in claim 3, characterized in that, The thickness at the opening point (322) is less than or equal to 0.2 mm.

5. The protective cover as described in claim 1, characterized in that, It also includes an abutment part (33), one end of which is connected to the inner wall of the housing (31) away from the tube body (1), and the abutment part (33) and the first section (311) of the housing (31) form a sealing groove (331) that opens toward the tube body (1), the opening of the tube body (1) is embedded in the sealing groove (331) and abuts against the inner wall of the sealing groove (331).

6. A cryopreservation tube, characterized in that, Includes the protective cap (3), tube (1) and cover (2) as described in any one of claims 1 to 5.

7. The cryopreservation tube as described in claim 6, characterized in that, A buckle (112) is provided on the outer wall of the tube (1), and a blocking part (313) that cooperates with the buckle (112) is provided on the inner wall of the first section (311) of the shell (31). When the first section (311) of the shell (31) is sleeved and connected to the opening of the tube (1), the buckle (112) is constrained by the blocking part (313).

8. The cryopreservation tube as described in claim 7, characterized in that, The opening of the tube (1) is connected to the first section (311) of the housing (31) by a first thread (111). The buckle (112) has an inclined surface that is inclined towards the outside of the tube (1) at one end away from the cover (2). The inner wall of the housing (31) near the tube (1) is formed with a blocking part (313) corresponding to the buckle (112). When the first section (311) of the housing (31) is connected to the opening of the tube (1), the buckle (112) abuts against the blocking part (313) and is located between the blocking part (313) and the thread.

9. The cryopreservation tube as described in claim 6, characterized in that, The cover (2) includes a mating part (21), a sealing part (22), and a closing part (23). The mating part (21) is mated to the second section (312) of the housing (31) and is detachably connected to the second section (312) of the housing (31). The sealing part (22) is connected to the end of the mating part (21) away from the tube (1) and is sleeved on the inner side of the mating part (21). The outer wall of the sealing part (22) abuts against the inner wall of the second section (312) of the housing (31). The edge of the closing part (23) is connected to the sealing part (22) and is used to close the end of the mating part (21) away from the tube (1).

10. The cryopreservation tube as described in claim 9, characterized in that, The closure (23) is recessed toward the side closer to the tube body (1).

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