Application of d-ribose in improving plant resistance to salt stress

Abstract

The application belongs to the technical field of biological breeding, and particularly relates to application of D-ribose in improving plant salt stress resistance. The application provides application of D-ribose in improving plant salt stress resistance. As an important plant metabolite, D-ribose plays a key role in the growth and development stage of plants. Under salt stress conditions, application of D-ribose can not only promote the growth of plant taproot, plant height and leaf, reduce the inhibitory effect of salt stress on crop growth, but also has a protective effect on flowering and seed production of later plants. The application method is simple, and the use effect is remarkable.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological breeding, and particularly relates to application of D-ribose in improving plant salt stress resistance. BACKGROUND

[0002] Excessive soil salt has a serious threat to plant growth and food production, and has a far-reaching impact on China's agricultural production and sustainable development. First, high salt environment can disturb the normal metabolism of plants, reduce their water absorption capacity, and further cause leaf stomata to close and bud growth to be blocked. In addition, excessive salt can inhibit the synthesis of chlorophyll and the activity of enzymes in the photosynthetic apparatus, especially the formation of chlorophyll, resulting in pale and yellowing of plant leaves, which seriously affects the synthesis of carbohydrates in photosynthesis, and further hinders the healthy growth and development of plants. At the same time, high salt also weakens the respiration of plants, increases the respiration consumption in the body, and reduces the net photosynthetic rate, which is further not conducive to plant growth.

[0003] Secondly, excessive salt can also cause ion toxicity effect, in which the intracellular accumulation of K + , Zn2+, Mn2+ and Mo2+ can cause damage to cell structure, including damage to membrane structure and organelles, affect the absorption of beneficial nutrients, change the process of photosynthesis, accelerate leaf senescence, and inhibit enzyme activity. With the increasing problem of soil salt, the damage to social economy and agricultural ecosystem is deepening, which has become a major obstacle to the development of China's agriculture. Therefore, how to effectively alleviate the problem of salt stress faced by plants has become an important issue to be solved.

[0004] Plants continuously interact with the environment at all stages of growth and development, during which a large number of primary and secondary metabolites are produced in plants. These metabolites exhibit specific or non-specific expression patterns in different developmental stages and tissue sites of plants, and act as key factors in response to various biological and abiotic stresses. Salt stress, as a significant abiotic stress, has a negative effect on plant growth, and can trigger dynamic adjustment of metabolite levels in plants. These adjustments involve various metabolites, such as basic metabolites such as amino acids and carbohydrates, and secondary metabolites such as phenolic acids, flavonoids, terpenes, steroids and alkaloids. Studies have shown that these metabolites play a core role in the mechanism of plant adaptation to biological and abiotic stresses. In particular, sucrose, as a key metabolite, plays an important role in the process of plant adaptation to salt stress. For example, under salt stress, the sucrose synthesis activity and sucrose content of sweet sorghum are significantly increased, which may mean that sucrose is involved in the response mechanism of plants to salt stress. This phenomenon reveals that the synthesis and content changes of metabolites in plants are adaptive responses of plants to adverse growth conditions. Therefore, exploring and utilizing these metabolites that change significantly under salt stress provide a new perspective and approach for developing plant salt tolerance solutions.

[0005] When soil salt significantly accumulates, excessive accumulation of reactive oxygen species occurs in plants, triggering oxidative stress. To resist the damage of excessive reactive oxygen species, plants need to activate a series of antioxidant defense mechanisms to maintain the redox balance in cells. In exploring how to improve plant yield and growth conditions under salt stress, it is crucial to explore which metabolites can protect plants from salt stress. The metabolites specifically expressed by plants under salt stress may be the key factors that play a key role in adversity. Therefore, under salt stress conditions, exogenous application of these specific metabolites provides a new strategy for plants to cope with salt stress. Reasonable use of these functional metabolites is expected to open up new ways to improve plant growth and development under salt stress conditions. However, current research on metabolites in plant salt stress response is still insufficient, and the functions of many potential metabolites in alleviating plant stress growth still need to be further explored. SUMMARY

[0006] The purpose of the present application is to provide the application of D-ribose in improving plant resistance to salt stress, which has broad application prospects.

[0007] To achieve the above purpose, the technical scheme adopted by the present application is:

[0008] The application of D-ribose in improving plant resistance to salt stress.

[0009] Further, the concentration of the salt stress is 50-200 mmol / L.

[0010] Further, the specific way of the application is:

[0011] D-ribose is applied to the seeds or seedlings of the plants.

[0012] Further, the application concentration of the D-ribose is 0.5-1.5 mmol / L.

[0013] Further, the application concentration of the D-ribose is 1 mmol / L.

[0014] Further, the specific method of applying the D-ribose to the seeds of the plants is to sterilize the seeds of the plants and then sow them in a culture medium containing D-ribose for culture.

[0015] Further, the specific method of applying the D-ribose to the seedlings of the plants is to add D-ribose to water or a culture medium and then perform foliar spraying treatment on the seedlings of the plants.

[0016] Further, the plants are Arabidopsis thaliana, cotton, corn, wheat, and soybean.

[0017] Further, the spraying frequency is 1 time / 5 days, and the spraying amount is sufficient to wet all the leaves.

[0018] Further, the D-ribose can promote the growth of the main roots, plant height, and leaves of the plants and effectively alleviate the inhibition of salt stress on the growth and development of the plants.

[0019] The beneficial technical effects of the application are:

[0020] The application provides application of D-ribose in improving plant salt stress resistance. As a plant metabolite, D-ribose can play an important role in the growth and development of plants. Under salt stress conditions, the application of D-ribose can promote the growth of the main roots, plant height, and leaves of crops, effectively reduce the negative effects of salt stress, and ensure the smooth progress of flowering and seed production in the later stage. Moreover, the application method is simple, and the use effect is remarkable. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a phenotype diagram of Arabidopsis thaliana plants cultured for 7 days using the three culture media in Example 1;

[0022] Figure 2 is a phenotype diagram of Arabidopsis thaliana plants cultured for different times in Example 2; wherein Figure 2 A is a phenotype diagram of Arabidopsis thaliana plants cultured for 10 days in Example 2, Figure 2 B is a phenotype diagram of Arabidopsis thaliana plants cultured for 60 days in Example 2;

[0023] Figure 3are the phenotype pictures of the cotton plants of each group after 14 days of culture in Example 3;

[0024] Figure 4 are the phenotype pictures of the corn plants of each group after 14 days of culture in Example 4. DETAILED DESCRIPTION

[0025] The following further describes the present application in connection with specific preferred embodiments, which are not to be construed as limiting the present application to only these described embodiments. Those skilled in the art will readily understand that modifications and substitutions can be made to the present application without departing from the concept thereof. The specific conditions not mentioned in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturers. The reagents or instruments used are conventional products obtained from the market, unless otherwise specified.

[0026] Example 1

[0027] Wild type (WT) Arabidopsis seeds were soaked in 75% alcohol for 5 minutes, then moved into 50% droplets for 10 minutes, and then the seeds were washed with sterile water for 5 times. The sterilized Arabidopsis seeds were sowed into petri dishes containing three kinds of solid culture medium, medium ① was 1 / 2MS basic medium, the pH of the medium was 5.7; medium ② was 1 / 2MS basic medium, the pH was 5.7, and it further included 100 mmol / L NaCl; medium ③ was 1 / 2MS basic medium, the pH was 5.7, and it further included 100 mmol / L NaCl and 1 mmol / L D-ribose. The petri dishes were sealed with sealing film to maintain sterility and appropriate humidity. The petri dishes were placed in a 4°C light-free vernalization treatment for 3 days. After the vernalization treatment, the petri dishes were moved to an Arabidopsis growth room and placed vertically, and cultured under long-day conditions at 22°C (16h light / 8h dark).

[0028] Example 2

[0029] The WT Arabidopsis plants grown on medium ① for 7 days were moved into soil for 7 days of culture. Then the WT Arabidopsis plants were grouped and cultured according to the methods shown in Table 1, and each group was cultured for 60 days.

[0030] Table 1

[0031]

[0032]

[0033] Example 3

[0034] The cotton seeds of Zhongmian 619 were germinated and then planted in the soil, and watered normally until the cotton plants grew true leaves. After the cotton plants grew true leaves, the cotton plants were grouped and cultured as shown in Table 2, with each group cultured for 14 days.

[0035] Table 2

[0036]

[0037] Example 4

[0038] B104 corn seeds were germinated and then planted in the soil until the corn grew true leaves. After the corn grew true leaves, the corn plants were grouped and cultured as shown in Table 3, with each group cultured for 14 days.

[0039] Table 3

[0040]

[0041] Experimental Example

[0042] Experimental Example 1

[0043] Phenotypic photographs were taken and observed of Arabidopsis thaliana plants obtained after culturing in three different culture media for 7 days in Example 1. Changes in their growth and development were analyzed, and the results are shown in [reference missing]. Figure 1 As shown.

[0044] Figure 1 The images show the phenotypic characteristics of Arabidopsis thaliana plants cultured for 7 days using the three culture media described in Example 1. (Observation) Figure 1 It was found that, compared to culture medium ①, culture of Arabidopsis thaliana plants using culture medium ② inhibited the growth of the taproot. Compared to culture medium ②, culture of Arabidopsis thaliana plants using culture medium ③ effectively alleviated the inhibitory effect of salt stress on the taproot growth of Arabidopsis thaliana. These results indicate that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on the taproot growth of Arabidopsis thaliana.

[0045] Experiment Example 2

[0046] Phenotypic photographs were taken and observed of Arabidopsis thaliana plants in each group after 10 and 60 days of culture in Example 2. Changes in their growth and development were analyzed. The results are shown in [link to results]. Figure 2 As shown.

[0047] Figure 2 These are phenotypic images of Arabidopsis thaliana plants from different groups after cultivation for different time periods in Example 2. Figure 2 A is a phenotypic diagram of Arabidopsis thaliana plants from each group after 10 days of culture in Example 2. Observation Figure 2As shown in Figure A, compared to the Control group, salt stress inhibited the leaf growth of Arabidopsis thaliana plants, and the inhibitory effect on leaf growth increased with increasing salt stress. Compared to various salt concentration stress groups, the groups with added D-ribose effectively alleviated the inhibitory effect of salt stress on Arabidopsis thaliana leaf growth. Figure 2 B is a phenotypic diagram of Arabidopsis thaliana plants from each group after 60 days of culture in Example 2. Observation Figure 2 As shown in Figure B, compared to the Control group, salt stress inhibited the plant height and leaf growth of Arabidopsis thaliana after 60 days of growth, and severely suppressed flowering and seed production. Furthermore, the inhibitory effect on plant height and leaf growth increased with increasing salt stress. Compared to various salt concentration stress groups, the D-ribose supplementation group effectively alleviated the inhibitory effect of salt stress on the overall plant growth of Arabidopsis thaliana in the later stages. These results indicate that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on the plant height and leaf growth of Arabidopsis thaliana, and has a protective effect on flowering and seed production in the later stages of Arabidopsis thaliana.

[0048] Experimental Example 3

[0049] Phenotypic photographs were taken of cotton plants in each group after 14 days of cultivation in Example 3, and their growth and development changes were analyzed. The results are shown in [link to results]. Figure 3 As shown.

[0050] Figure 3 These are phenotypic images of cotton plants in each group after 14 days of cultivation in Example 3. Observation Figure 3 It was found that, compared with the Control group, salt stress inhibited the plant height and leaf growth of cotton, and the inhibitory effect on plant height and leaf growth increased with the increase of salt stress. Compared with various salt concentration stress groups, the addition of D-ribose effectively alleviated the inhibitory effect of salt stress on cotton plant height and leaf growth. The above results indicate that the addition of D-ribose can effectively alleviate the inhibitory effect of salt stress on cotton leaf growth.

[0051] Experiment Example 4

[0052] Phenotypic photographs were taken of maize plants in each group after 14 days of cultivation in Example 4, and their growth and development changes were analyzed. The results are shown in [link to example]. Figure 4 As shown.

[0053] Figure 4 These are phenotypic images of maize plants in each group after 14 days of cultivation in Example 4. Observation Figure 4It can be seen that, compared with the Control group, under salt stress, the plant height and leaf growth of corn were inhibited, and the inhibition on the plant height and leaf growth of corn was enhanced with the increase of salt stress. Compared with various salt concentration stress groups, the addition of D-ribose effectively alleviated the inhibition of salt stress on the plant height and leaf growth of corn. The above results show that the addition of D-ribose can effectively alleviate the inhibition of salt stress on the plant height and leaf growth of corn. In summary, salt stress seriously damages the normal growth and development of the main root, plant height and leaf of the plant, and inhibits the overall growth of the plant, and further affects the flowering and seed formation in the later period, and the inhibition effect is intensified with the deepening of the degree of salt stress. The addition of D-ribose can actively promote the growth of the main root, plant height and leaf of the crop, effectively reduce the negative impact of salt stress, and at the same time ensure the smooth progress of the later flowering and seed production.

[0054] Finally, it should be pointed out that the above examples are only used to illustrate the technical solutions of the present application, but not to limit it. The basic principles and main features of the present application have been described above with specific embodiments, and some modifications or substitutions can be made on the basis of the present application, but these modifications or substitutions do not make the corresponding technical solutions deviate from the scope of the present application.

Claims

1. Application of D-ribose in improving plant salt stress resistance.

2. The application of D-ribose according to claim 1 in improving plant salt stress resistance, characterized in that, The concentration of the salt stress was 50-200 mmol / L.

3. The application of D-ribose according to claim 1 in improving plant salt stress resistance, characterized in that, The specific method of application is as follows: Apply D-ribose to the seeds or seedlings of plants.

4. The application of D-ribose according to claim 3 in improving plant salt stress resistance, characterized in that, The concentration of D-ribose applied is 0.5-1.5 mmol / L.

5. The application of D-ribose according to claim 4 in improving plant salt stress resistance, characterized in that, The concentration of D-ribose administered was 1 mmol / L.

6. The application of D-ribose according to claim 3 in improving plant salt stress resistance, characterized in that, The specific method for applying D-ribose to plant seeds is as follows: sterilize the plant seeds and then sow them in a culture medium containing D-ribose for cultivation.

7. The application of D-ribose according to claim 3 in improving plant salt stress resistance, characterized in that, The specific method for applying D-ribose to plant seedlings is as follows: add D-ribose to water or culture medium, and then spray the seedlings with the solution.

8. The application of D-ribose according to claim 7 in improving plant salt stress resistance, characterized in that, The plants mentioned are Arabidopsis thaliana, cotton, corn, wheat, and soybean.

9. The application of D-ribose according to claim 7 in improving plant salt stress resistance, characterized in that, The spraying frequency is once every 5 days; the amount of spraying is sufficient to completely wet all leaves.

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