Composition containing pig brain protein hydrolysate as well as preparation method and application thereof

Abstract

The invention discloses a composition containing pig cerebroprotein hydrolysate, and a preparation method and application thereof. The composition comprises pig brain protein hydrolysate, gastrodia elata, American ginseng, dogwood, ginkgo, rhizoma polygonati, radix puerariae, folium mori, radix asparagi, vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12. The composition can improve memory, further repair damaged nerve cells, improve brain blood circulation, reduce damage caused by oxygen free radicals, accelerate removal of free radicals in the body, eliminate inflammation and maintain nervous system health, so that the composition has the effects of improving memory and preventing Alzheimer's disease.

Description

Technical Field

[0001] The present invention relates to the technical field of composition preparation, and more specifically, to a composition, a preparation method thereof and application thereof, wherein the composition is preferably a food composition. Background Art

[0002] Alzheimer's disease (AD) was first proposed by German doctor Alois Alzheimer in 1906. It is a degenerative disease of the central nervous system that occurs in the elderly and is characterized by progressive cognitive dysfunction and behavioral impairment, accounting for 70% of dementia. The main clinical manifestations of AD are memory impairment, aphasia, agnosia, visual-spatial skill impairment, executive dysfunction, and personality and behavioral changes. To date, AD has two main characteristics: a large number of patients and a heavy burden on society and families.

[0003] As of the end of 2021, there are 51.62 million registered patients worldwide, of which 15 million are in my country, ranking first in the world in absolute terms. It is estimated that by 2050, the number of patients worldwide will increase to 139 million. What is more serious is that as my country gradually enters an aging society, the number of elderly people has begun to rise significantly. As of the end of 2017, there were 241 million elderly people aged 60 and above in my country, accounting for 17.3% of the total population (according to international practice, if the elderly population aged 60 and above accounts for 10% of the total population, it means entering an aging society). It is estimated that by around 2050, the number of elderly people in my country will reach a peak of 487 million, accounting for 34.9% of the total population, which objectively increases the potential population of AD patients in my country.

[0004] AD has a long course (cases as short as three to five years and as long as 20 to 30 years have been reported), making AD patients a serious burden on society and families. According to statistics from the World Health Organization, the current global cost of caring for AD patients is higher than the sum of cardiovascular disease, cancer and stroke. According to a national study, the annual treatment cost of AD patients in China in 2015 was US$167.74 billion (RMB 1.1 trillion / year), and it is expected to increase to US$1.8 trillion (RMB 12.6 trillion / year) by 2050. Therefore, if this disease is not controlled, its various direct and implicit expenses will impose a huge burden on every family and the entire society.

[0005] However, there is no drug that can completely cure AD, because the exact pathogenesis of AD has not been fully studied. At this stage, AD patients can only delay the progression of the disease as much as possible and improve their quality of life through "early diagnosis, early detection, and early intervention". According to the calculations of relevant authoritative departments, if the age of onset of Alzheimer's disease can be delayed by 10 years through intervention, the number of patients in the next generation will be reduced by 90%, which will reduce a huge burden on society.

[0006] Although the pathogenesis of AD is unclear, the clinical molecular biological characteristics of AD are clear, mainly manifested in oxidative damage to nerve cells, inflammation and reduction of neurotransmitters, and the resulting decline in memory and learning ability.

[0007] In the health products or medicines based on brain protein hydrolysate at present, although there is a certain effect in improving memory, due to its single composition, it causes its limited effect, the improvement degree of memory is unstable, the onset time is long, the drug effect time is short, and drug resistance is easy to occur. If the dosage of such health products or medicines is increased rashly, obvious side effects will be caused again. Because the brain protein hydrolysate contains exciting active ingredients, overdose can cause mental excitement or tension, skin itching and rash, and some exciting effects are produced with the generation of, for example, excessive behavior, tension, insomnia, hyperventilation, hypertension, hypotension, fatigue, tremor, depression, indifference, numbness, cold-like symptoms (chills, cough, respiratory tract infection) etc. Therefore, how to enhance the improvement effect of brain protein hydrolysate on memory is a problem that is urgently needed to be solved. Summary of the invention

[0008] Technical issues

[0009] Although the health products or drugs based on brain protein hydrolysate have certain effects in improving memory, their single ingredients have limited effects, unstable improvement in memory, long onset time, short duration of efficacy, and easy to develop drug resistance. If the dosage of such health products or drugs is increased rashly, it will cause obvious side effects.

[0010] Technical Solution

[0011] By combining protein hydrolysate, plant raw materials and vitamins, the improvement effect of memory is enhanced and side effects are avoided; by combining plant raw materials and vitamins, damaged nerve cells can be further repaired; it can improve brain blood circulation, reduce damage caused by oxygen free radicals, accelerate the removal of free radicals in the body and eliminate inflammation. The composition of the present invention replenishes qi and produces blood, calms the nerves; improves the digestive function and metabolic function of the user, and allows the nutrients in the composition to be fully absorbed by the human body. Assisted by the antioxidant effect of vitamin C and vitamin B5, and the effect of maintaining the health of the nervous system of vitamin B1, vitamin B6 and vitamin B12, the entire formula composition has the effect of improving memory and preventing and treating Alzheimer's disease, and has good application prospects.

[0012] To achieve the above object, the present invention provides a composition in the first aspect, which comprises the following raw materials: 10-100 parts by weight of porcine brain protein hydrolyzate; a plant concentrate prepared from 3000-15000 parts by weight of Gastrodia elata, 1000-8000 parts by weight of American ginseng, 2000-15000 parts by weight of Cornus officinalis, 5000-10000 parts by weight of Ginkgo, 3000-25000 parts by weight of Polygonatum sibiricum, 2000-23000 parts by weight of Pueraria lobata (preferably Pueraria lobata), 2000-10000 parts by weight of mulberry leaf, and 2000-20000 parts by weight of Asparagus cochinchinensis; and 10-35 parts by weight of vitamin C, 0.1-0.4 parts by weight of vitamin B1, 1.5-5 parts by weight of vitamin B3, 0.3-1.5 parts by weight of vitamin B5, 0.1-0.5 parts by weight of vitamin B6, and 0.1×10 -3 -1.0×10 -3 Parts by weight.

[0013] In one embodiment of the first aspect of the present invention, the composition comprises the following raw materials: 10-100 mg / portion of porcine brain protein hydrolysate; a plant concentrate made from 3-15 g / portion of Gastrodia elata, 1-8 g / portion of American ginseng, 2-15 g / portion of Cornus officinalis, 5-10 g / portion of Ginkgo biloba, 3-25 g / portion of Polygonatum sibiricum, 2-23 g / portion of Pueraria lobata (preferably Pueraria lobata), 2-10 g / portion of Morus alba, and 2-20 g / portion of Asparagus cochinchinensis; 10-35 mg / portion of vitamin C, 0.1-0.4 mg / portion of vitamin B1, 1.5-5 mg / portion of vitamin B3, 0.3-1.5 mg / portion of vitamin B5, 0.1-0.5 mg / portion of vitamin B6, and 0.1-1.0 μg / portion of vitamin B12.

[0014] In one embodiment of the first aspect of the present invention, the composition is a food composition, preferably a food composition for preventing Alzheimer's disease.

[0015] In a second aspect of the present invention, a method for preparing the composition of the present invention is provided, comprising the following steps:

[0016] S1. Cut the pig brain into pieces and steam, separate the oil and water from the steamed liquid, and hydrolyze the aqueous phase with a composite enzyme preparation consisting of trypsin, chymotrypsin and alkaline protease to obtain a collagen peptide solution, which is spray-dried to obtain a pig brain protein hydrolyzate, labeled as "animal dry powder", and placed in a container for standby use;

[0017] S2. The solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12 are uniformly mixed in the proportions described in the first aspect above, and passed through an 80-mesh sieve, marked as "vitamin excipients", and placed in a container for use;

[0018] S3. Prepare plant-derived ingredients by mixing Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root (preferably kudzu root) and asparagus cochinchinensis according to the ratio described in the first aspect above to prepare a mixed extract, which is concentrated and marked as "plant concentrate" and placed in a container for use;

[0019] S4. The "animal dry powder" prepared in S1 and the "plant concentrate" prepared in S3 are mixed, and then the "vitamin excipient" prepared in S2 is added to the mixture, and the preparation of the finished composition is completed by granulation and drying.

[0020] In one embodiment of the second aspect of the present invention, S4 further comprises adding one or more of the following excipients or additives to the finished composition: diluents, excipients, lubricants, disintegrants, flavoring agents, binders, fillers, preservatives, surfactants, colorants, emulsifiers, suspending agents, gelling agents, pH adjusters and solubilizers.

[0021] In one embodiment of the second aspect of the present invention, the dosage form of the finished composition includes but is not limited to solid beverages, oral liquids, compressed candies, soft capsules, and the like.

[0022] In one embodiment of the second aspect of the present invention, the steps of preparing the porcine brain protein hydrolysate in S1 are as follows:

[0023] S1. First, the fresh pig brain is manually removed of impurities, and then the brain is pulverized into 2-3 cm tissue blocks using a block cutter, and then the pulverized tissue blocks are cleaned with water, and then placed in a cooking tank and water is added for cooking, wherein the volume ratio of the tissue block to water is 1:2-1:5, the cooking temperature is 80-120°C, the cooking pressure is 1-3MPa, and the cooking time is 2-6 hours to obtain a cooking liquid;

[0024] S2. The cooking liquid obtained in S1 is separated into oil and water by gravity separation, and the resulting aqueous phase is cooled to 50 to 55° C. to obtain a crude collagen solution;

[0025] S3. Add the composite enzyme preparation to the crude collagen solution obtained in S2, mix well and perform enzymolysis for 0.5-3 hours to obtain a collagen peptide solution, wherein the composite enzyme preparation is composed of trypsin, chymotrypsin and alkaline protease;

[0026] S4. Filtering the collagen peptide solution obtained in S3 to remove tissue fragments and other impurities, and then filtering with an inorganic nanofiltration ceramic membrane with a molecular weight cutoff of 800Da to further obtain purified collagen peptides;

[0027] S5. Concentrate the purified collagen peptides obtained in S4, spray-dry, and obtain the final porcine brain protein hydrolysate.

[0028] In one embodiment of the second aspect of the present invention, the preparation steps of the mixed extract of Gastrodia elata, American ginseng, Ginkgo biloba, Cornus officinalis, Polygonatum sibiricum, Morus alba, Pueraria lobata (preferably Pueraria lobata) and Asparagus cochinchinensis in S3 are as follows:

[0029] S1. Cut the dried Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root (preferably kudzu root) and asparagus into small pieces of 2 to 3 mm, and the cutting is completed by a knife or a cutting machine;

[0030] S2. Add the cut Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaf, kudzu root (preferably kudzu root) and asparagus root into a sealed extraction tank according to the proportions described in the first aspect, add purified water at a mass volume ratio of 1:1-1:10, extract at 70-130° C. for 1-8 hours, and filter to obtain the supernatant. Repeat the extraction 2-5 times.

[0031] S3. Collect all the supernatant and concentrate it to obtain "plant concentrate", which is then placed in a container for later use.

[0032] In one embodiment of the second aspect of the present invention, the composition is a food composition, preferably a food composition for treating and preventing Alzheimer's disease.

[0033] The third aspect of the present invention provides use of the composition containing brain protein hydrolysate according to the first aspect of the present invention or the composition containing brain protein hydrolysate prepared by the method of the second aspect of the present invention in preparing a composition for treating and preventing Alzheimer's disease.

[0034] Beneficial effects:

[0035] The present invention adopts a combination of plant raw materials, vitamins and brain protein hydrolysate, and can promote the absorption of brain protein hydrolysate, reduce the side effects of brain protein hydrolysate, and further repair damaged nerve cells through the synergistic effect of plant raw materials and brain protein hydrolysate; can improve brain blood circulation, reduce damage caused by oxygen free radicals, accelerate the removal of free radicals in the body and eliminate inflammation. The composition of the present invention replenishes qi and produces blood, calms the nerves; improves the digestive function and metabolic function of the user, and allows the nutrients in the composition to be fully absorbed by the human body. Assisted by the antioxidant effect of vitamin C and vitamin B5, and the effect of maintaining the health of the nervous system of vitamin B1, vitamin B6 and vitamin B12, the entire formula composition has the effect of improving memory and preventing and treating Alzheimer's disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is shown that the five formula products of the present invention have no toxicity to PC12 cells, but instead have a certain nutritional effect of promoting growth.

[0037] Figure 2 It was shown that the five formulations of the present invention had no significant effect on the body weight of mice, indicating that they are biosafe.

[0038] Figure 3 It is shown that the five formulations of the present invention all have the effect of reducing IL-6 in the brain tissue of mice, indicating that they have anti-inflammatory effects.

[0039] Figure 4 It is shown that the five formulations of the present invention all have the effect of reducing ROS in the brain tissue of mice, indicating that they have an antioxidant effect.

[0040] Figure 5 It was shown that the five formulations of the present invention had a significant improvement effect on the behavior of 5*FAD transgenic mice, indicating that they have the effect of treating Alzheimer's disease. DETAILED DESCRIPTION

[0041] The technical scheme in the embodiment of the present invention will be clearly and completely described below. Obviously, the described embodiment is only a part of the embodiment of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If the specific conditions are not specified in the embodiment, they are carried out according to the conditions recommended by the conventional conditions or the manufacturer. If the manufacturer is not specified in the reagents or instruments used, they are all conventional products that can be obtained commercially.

[0042] The composition of the invention comprises: pig brain protein hydrolysate, gastrodia elata, American ginseng, cornus officinalis, ginkgo, polygonatum, kudzu root, mulberry leaf, asparagus cochinchinensis, vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12.

[0043] In one embodiment of the present invention, the Gastrodia elata, American ginseng, Cornus officinalis, Ginkgo biloba, Polygonatum sibiricum, Pueraria root, Morus alba and Asparagus cochinchinensis contained in the present invention are plant concentrates.

[0044] In one embodiment of the present invention, the plant concentrate is obtained by mixing Gastrodia elata, American ginseng, Cornus officinalis, Ginkgo biloba, Polygonatum sibiricum, Pueraria root, Morus alba and Asparagus cochinchinensis, and then extracting with water.

[0045] In one embodiment of the present invention, the method for preparing the plant concentrate comprises the following steps:

[0046] S1. Cut the dried Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root and asparagus into small pieces of 2 to 3 mm, and the cutting is completed by a knife or a cutting machine;

[0047] S2. Add the cut pieces of Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root and asparagus to a closed extraction tank, add purified water according to a mass volume ratio of 1:1-1:10, extract at 70-130 ° C for 1-8 hours, filter to obtain the supernatant, and repeat the extraction 2-5 times;

[0048] S3. Collect all the supernatant and concentrate it to obtain "plant concentrate", which is then placed in a container for later use.

[0049] The plant concentrate may be a product obtained by concentrating the supernatant, or may be a dry powder obtained by further drying.

[0050] Depending on the concentration degree, the product obtained after the supernatant is concentrated may contain a water content of less than 50% by weight, a water content of less than 40% by weight, a water content of less than 30% by weight, a water content of less than 20% by weight, a water content of less than 10% by weight, or a water content of less than 5% by weight. Depending on the drying degree, the dry powder may contain a water content of less than 4% by weight, a water content of less than 3% by weight, a water content of less than 2% by weight, or a water content of less than 1% by weight.

[0051] In one embodiment of the present invention, the mixing ratio of the plant raw materials is: 3000 to 15000 parts by weight of Gastrodia elata, 1000 to 8000 parts by weight of American ginseng, 2000 to 15000 parts by weight of Cornus officinalis, 5000 to 10000 parts by weight of Ginkgo, 3000 to 25000 parts by weight of Polygonatum sibiricum, 2000 to 23000 parts by weight of Pueraria root, 2000 to 10000 parts by weight of mulberry leaf, and 2000 to 20000 parts by weight of Asparagus cochinchinensis.

[0052] In one embodiment of the present invention, the Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum sibiricum, mulberry leaf, kudzu root and asparagus cochinchinensis are selected from fresh, complete and pest-free parts;

[0053] In one embodiment of the present invention, Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaf, kudzu root and asparagus cochinchinensis are washed with clean water and then dried, and the drying is selected from natural drying or hot air drying.

[0054] In one embodiment of the present invention, the composition of the present invention comprises: 10 to 100 parts by weight of porcine brain protein hydrolyzate; a plant concentrate prepared from 3000 to 15000 parts by weight of Gastrodia elata, 1000 to 8000 parts by weight of American ginseng, 2000 to 15000 parts by weight of Cornus officinalis, 5000 to 10000 parts by weight of Ginkgo, 3000 to 25000 parts by weight of Polygonatum sibiricum, 2000 to 23000 parts by weight of Pueraria root, 2000 to 10000 parts by weight of mulberry leaf, and 2000 to 20000 parts by weight of Asparagus cochinchinensis; and 10 to 35 parts by weight of vitamin C, 0.1 to 0.4 parts by weight of vitamin B1, 1.5 to 5 parts by weight of vitamin B3, 0.3 to 1.5 parts by weight of vitamin B5, and 0.6 to 1.5 parts by weight of vitamin B6. 0.1 to 0.5 parts by weight, Vitamin B12 0.1×10 -3 Weight parts to 1.0×10 -3 Parts by weight.

[0055] In one embodiment of the present invention, the composition comprises: 10-100 mg / portion of porcine brain protein hydrolyzate; a plant concentrate prepared from 3-15 g / portion of Gastrodia elata, 1-8 g / portion of American ginseng, 2-15 g / portion of Cornus officinalis, 5-10 g / portion of Ginkgo biloba, 3-25 g / portion of Polygonatum sibiricum, 2-23 g / portion of Pueraria lobata (preferably Pueraria lobata), 2-10 g / portion of Morus alba, and 2-20 g / portion of Asparagus cochinchinensis; 10-35 mg / portion of vitamin C, 0.1-0.4 mg / portion of vitamin B1, 1.5-5 mg / portion of vitamin B3, 0.3-1.5 mg / portion of vitamin B5, 0.1-0.5 mg / portion of vitamin B6, and 0.1-1.0 μg / portion of vitamin B12.

[0056] Although cerebroprotein hydrolysate has certain efficacy in improving memory, due to its single composition, it causes its limited effect, the improvement degree of memory is unstable, the onset time is long, the drug effect time is short, and drug resistance occurs easily. And cerebroprotein hydrolysate contains exciting active ingredients, excessive taking can cause mental excitement or tension, skin itching and rash occur, and some exciting effects are produced with, for example, excessive behavior, tension, insomnia, hyperventilation, hypertension, hypotension, weakness, tremor, depression, coldness, numbness, cold-like symptoms (chills, cough, respiratory tract infection) etc. Therefore, the present invention adopts plant raw materials and vitamins and cerebroprotein hydrolysate combination, can overcome the above-mentioned problems it has.

[0057] In one embodiment of the present invention, the plant raw materials are selected from Gastrodia elata, American ginseng, Cornus officinalis, Ginkgo, Polygonatum sibiricum, Pueraria root, mulberry leaf, and Asparagus cochinchinensis; and the vitamins are selected from vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6, and vitamin B12.

[0058] In particular, the present invention uses a combination of plant raw materials, vitamins and brain protein hydrolysate, which can promote the absorption of brain protein hydrolysate, reduce the side effects of brain protein hydrolysate, and further repair damaged nerve cells through the synergistic effect of plant raw materials and brain protein hydrolysate; it can improve brain blood circulation, reduce damage caused by oxygen free radicals, accelerate the removal of free radicals in the body and eliminate inflammation. Therefore, the composition of the present invention can be used for all diseases and symptoms used for brain protein hydrolysate, and eliminate the side effects of brain protein hydrolysate.

[0059] The composition of the present invention replenishes Qi and blood, calms the nerves, improves the digestive function and metabolic function of the user, and allows the nutrients in the composition to be fully absorbed by the human body. In addition, with the antioxidant effect of vitamin C and vitamin B5, and the effect of vitamin B1, vitamin B6 and vitamin B12 in maintaining the health of the nervous system, the entire formula composition has the effect of improving memory and preventing and treating Alzheimer's disease.

[0060] In one embodiment of the present invention, the composition is a food composition.

[0061] In one embodiment of the present invention, the present invention provides use of the composition or the composition prepared by the method of the present invention in preparing a medicament for treating and preventing Alzheimer's disease.

[0062] The preparation and effects of the present invention are specifically explained below through examples.

[0063] Example:

[0064] Preparation Example 1: Preparation of porcine brain protein hydrolysate

[0065] The preparation steps of porcine brain protein hydrolysate are as follows:

[0066] S1. First, the fresh pig brain is manually removed of impurities, and then the brain is pulverized into 2-3 cm tissue blocks using a block cutter, and then the pulverized tissue blocks are cleaned with water, and then placed in a cooking tank and water is added for cooking, wherein the volume ratio of the tissue block to water is 1:2-1:5, the cooking temperature is 80-120°C, the cooking pressure is 1-3MPa, and the cooking time is 2-6 hours to obtain a cooking liquid;

[0067] S2. The cooking liquid obtained in S1 is separated into oil and water by gravity separation, and the resulting aqueous phase is cooled to 50 to 55° C. to obtain a crude collagen solution;

[0068] S3. Add the composite enzyme preparation to the crude collagen solution obtained in S2, mix well and perform enzymolysis for 0.5-3 hours to obtain a collagen peptide solution, wherein the composite enzyme preparation is composed of trypsin, chymotrypsin and alkaline protease;

[0069] S4. Filtering the collagen peptide solution obtained in S3 to remove tissue fragments and other impurities, and then filtering with an inorganic nanofiltration ceramic membrane with a molecular weight cutoff of 800Da to further obtain purified collagen peptides;

[0070] S5. Concentrate the purified collagen peptides obtained in S4, spray-dry, and obtain the final porcine brain protein hydrolysate.

[0071] Preparation Example 2: Preparation of plant concentrate

[0072] Select the complete and pest-free parts of fresh Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum sibiricum, mulberry leaf, kudzu root and asparagus cochinchinensis, wash them with clean water, dry them, and then obtain the plant concentrate through the following steps.

[0073] S1. Cut the dried Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root and asparagus into small pieces of 2 to 3 mm, and the cutting is completed by a knife or a cutting machine;

[0074] S2. The cut pieces of Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root and asparagus root were added to a closed extraction tank according to the ratio of the present invention, and purified water was added according to a mass volume ratio of 1:1-1:10, and extracted at 70-130 ° C for 1-8 hours, filtered to obtain the supernatant, and the extraction was repeated 2-5 times;

[0075] S3. Collect all the supernatant and concentrate it to a water content of 5% to obtain the "plant concentrate", which is placed in a container for later use.

[0076] Preparation Example:

[0077] Formulation 1: the raw materials include 10 mg / portion of porcine brain protein hydrolyzate; a plant concentrate prepared from 3 g / portion of Gastrodia elata, 1 g / portion of American ginseng, 2 g / portion of Cornus officinalis, 5 g / portion of Ginkgo biloba, 3 g / portion of Polygonatum sibiricum, 2 g / portion of Pueraria root (preferably Pueraria lobata), 2 g / portion of Morus alba and 2 g / portion of Asparagus cochinchinensis; 10 mg / portion of vitamin C, 0.1 mg / portion of vitamin B1, 1.5 mg / portion of vitamin B3, 0.3 mg / portion of vitamin B5, 0.1 mg / portion of vitamin B6 and 0.1 μg / portion of vitamin B12.

[0078] Formulation 2: The raw materials include 20 mg / portion of porcine brain protein hydrolyzate; a plant concentrate prepared from 6 g / portion of Gastrodia elata, 2 g / portion of American ginseng, 4 g / portion of Cornus officinalis, 6 g / portion of Ginkgo biloba, 6 g / portion of Polygonatum sibiricum, 4 g / portion of Pueraria root (preferably Pueraria lobata root), 4 g / portion of Morus alba and 3 g / portion of Asparagus cochinchinensis; 15 mg / portion of vitamin C, 0.2 mg / portion of vitamin B1, 2.0 mg / portion of vitamin B3, 0.5 mg / portion of vitamin B5, 0.2 mg / portion of vitamin B6 and 0.2 μg / portion of vitamin B12.

[0079] Formulation 3: The raw materials include 30 mg / portion of porcine brain protein hydrolyzate; a plant concentrate prepared from 9 g / portion of Gastrodia elata, 4 g / portion of American ginseng, 6 g / portion of Cornus officinalis, 8 g / portion of Ginkgo biloba, 9 g / portion of Polygonatum sibiricum, 6 g / portion of Pueraria lobata (preferably Pueraria lobata), 6 g / portion of Morus alba and 5 g / portion of Asparagus cochinchinensis; 20 mg / portion of vitamin C, 0.3 mg / portion of vitamin B1, 3.0 mg / portion of vitamin B3, 0.9 mg / portion of vitamin B5, 0.3 mg / portion of vitamin B6 and 0.4 μg / portion of vitamin B12.

[0080] Formulation 4: The raw materials include 50 mg / portion of porcine brain protein hydrolyzate; a plant concentrate prepared from 12 g / portion of Gastrodia elata, 6 g / portion of American ginseng, 8 g / portion of Cornus officinalis, 9 g / portion of Ginkgo biloba, 12 g / portion of Polygonatum sibiricum, 8 g / portion of Pueraria lobata (preferably Pueraria lobata), 8 g / portion of Morus alba and 9 g / portion of Asparagus cochinchinensis; 30 mg / portion of vitamin C, 0.3 mg / portion of vitamin B1, 4.0 mg / portion of vitamin B3, 1.2 mg / portion of vitamin B5, 0.4 mg / portion of vitamin B6 and 0.5 μg / portion of vitamin B12.

[0081] Formulation 5: The raw materials include a plant concentrate prepared from 100 mg / portion of porcine brain protein hydrolysate, 15 g / portion of Gastrodia elata, 8 g / portion of American ginseng, 15 g / portion of Cornus officinalis, 10 g / portion of Ginkgo biloba, 25 g / portion of Polygonatum sibiricum, 23 g / portion of Pueraria root (preferably Pueraria lobata), 10 g / portion of mulberry leaf and 20 g / portion of Asparagus cochinchinensis; 35 mg / portion of vitamin C, 0.4 mg / portion of vitamin B1, 5 mg / portion of vitamin B3, 1.5 mg / portion of vitamin B5, 0.5 mg / portion of vitamin B6 and 1.0 μg / portion of vitamin B12.

[0082] Preparation method:

[0083] The solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12 are mixed evenly and passed through an 80-mesh sieve to form a vitamin auxiliary material; then, the dry powder of pig brain protein hydrolyzate and the plant concentrate are added to the auxiliary material, and the preparation of the finished composition is completed by granulation and drying.

[0084] Experimental example

[0085] The five compositions prepared above were tested:

[0086] Experimental Example 1: PC12 cells (rat adrenal medullary pheochromocytoma differentiation cell line) are a neural cell model used to study more biological toxicity. This experimental example used the MTT colorimetric method to analyze the neurotoxicity of five formula products on PC12 cells. The detection principle is based on the fact that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to water-insoluble blue-purple crystalline formazan and deposit it in the cells. Dead cells cannot undergo this reaction. By using dimethyl sulfoxide (DMSO) to dissolve the formazan and measuring its light absorption value at a wavelength of 490nm using an enzyme-linked immunosorbent assay, the number of living cells can be indirectly reflected. Specifically, PC12 cells were cultured in DMEM medium for 7 days, and each formulation of the present invention was added at a ratio of 1 gram of product / 100 ml of culture medium, and then cultured for another 7 days. The cell survival rate of each group was determined by measuring its light absorption value at a wavelength of 490 nm, thereby determining the toxicity of each formulation to the cells. Figure 1 As shown, compared with the group without formula products, the five formula products showed no toxicity to nerve cells, but instead showed a certain nutritional effect in promoting the growth of nerve cells.

[0087] Experimental Example 2: 60 mice were randomly divided into 6 groups, with 10 mice in each group. In this experimental example, the control and the five formulations were fed to the mice twice a day, and the changes in the weight of the mice were monitored for 14 days to determine the toxicity of the product to the organism. Figure 2As shown, compared with the group without formula products, the five formula products did not show significant effects on the body weight of mice, indicating that the above products are biosafe.

[0088] Experimental Example 3: In this experimental example, the mice in Experimental Example 2 were euthanized in accordance with experimental ethics after the feeding was completed, and the concentration of interleukin-6 (IL-6) mRNA in their brain tissue was measured. Figure 3 As shown, the results showed that compared with the control, the five formula products all had a reducing effect on IL-6 in the mouse brain tissue, indicating that the above products have an anti-inflammatory effect.

[0089] Experimental Example 4: In this experiment, the mice in Experimental Example 2 were euthanized in accordance with experimental ethics, and the concentration of reactive oxygen free radicals (ROS) in their brain tissue was measured. Figure 4 As shown, the results showed that compared with the control, the five formula products all had a reducing effect on ROS in mouse brain tissue, indicating that the above products have an antioxidant effect.

[0090] Experimental Example 5: The Morris water maze experiment is a common method for evaluating the spatial learning and memory ability of rodents. Through the two stages of training and exploration, the spatial cognitive ability of animals can be fully understood. 5*FAD transgenic is a transgenic animal model widely used in the study of Alzheimer's disease. The present invention simultaneously trains wild-type mice, 5*FAD transgenic mice, and 5*FAD transgenic mice fed with five formula products, with a daily feeding amount of 200 mg of product / kg body weight, once a day, for 7 days, and then puts the above mice into water, and records the time and path they find the platform. Specifically, 7 mice were trained for 5 days, once a day, and the platform was withdrawn on the 6th day of training. The learning and memory behavior of the mice was measured, and the mice were placed on the opposite side of the platform facing the pool wall. The camera system records the movement time and trajectory of the mice in each quadrant. Figure 5 The movement trajectory of mice within 60 seconds on the 6th day is shown in the figure. It can be seen that the composition of the present invention has a significant improvement effect on the behavior of 5*FAD transgenic mice. In particular, the path taken by mice treated with the composition of the present invention to find the platform is significantly shorter and more direct than that of the control group.

[0091] According to Experimental Examples 1 to 5, it can be confirmed that the composition of the present invention has no toxicity to the subjects, and exhibits a certain nutritional effect on promoting the growth of PC12 cells representing neural cells; it can reduce oxidative damage and inflammation in brain tissue; and it has a significant improvement effect on the behavior of 5*FAD transgenic mice, indicating that it has the effect of improving memory and preventing and treating Alzheimer's disease.

Claims

1. A composition, wherein It contains the following ingredients in the following proportions: 10 to 100 parts by weight of porcine brain protein hydrolysate; A plant concentrate made from 3000 to 15000 parts by weight of Gastrodia elata, 1000 to 8000 parts by weight of American ginseng, 2000 to 15000 parts by weight of Cornus officinalis, 5000 to 10000 parts by weight of Ginkgo biloba, 3000 to 25000 parts by weight of Polygonatum sibiricum, 2000 to 23000 parts by weight of Pueraria root, 2000 to 10000 parts by weight of Morus alba and 2000 to 20000 parts by weight of Asparagus cochinchinensis; as well as Vitamin C 10 to 35 parts by weight, vitamin B1 0.1 to 0.4 parts by weight, vitamin B3 1.5 to 5 parts by weight, vitamin B5 0.3 to 1.5 parts by weight, vitamin B6 0.1 to 0.5 parts by weight and vitamin B12 0.1×10 -3 Weight parts to 1.0×10 -3 Parts by weight.

2. The composition according to claim 1, wherein The composition is a food composition.

3. A method for preparing the composition of claim 1, wherein: The following steps are involved: S1. The pig brain was cut into pieces and steamed, the obtained cooking liquid was separated into oil and water, and the aqueous phase was hydrolyzed with a composite enzyme preparation consisting of trypsin, chymotrypsin and alkaline protease to obtain a collagen peptide solution, which was spray-dried to obtain a pig brain protein hydrolyzate, marked as "animal dry powder", and placed in a container for use; S2. The solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12 are uniformly mixed in the proportions according to claim 1, and passed through an 80-mesh sieve, marked as "vitamin excipients", and placed in a container for use; S3. Prepare plant-derived ingredients, according to the ratio of claim 1, mix Gastrodia elata, American ginseng, ginkgo, Cornus officinalis, Polygonatum sibiricum, mulberry leaf, Pueraria lobata and Asparagus cochinchinensis to prepare a mixed extract, which is concentrated and marked as "plant concentrate" and placed in a container for use; S4. The "animal dry powder" prepared in S1 and the "plant concentrate" prepared in S3 are mixed, and then the "vitamin excipient" prepared in S2 is added to the mixture, and the finished composition is prepared by granulation and drying.

4. The method according to claim 3, wherein: The S4 also includes adding one or more of the following additives to the finished composition: diluents, excipients, lubricants, binders, fillers, preservatives, surfactants, colorants, flavoring agents, emulsifiers, suspending agents, gelling agents, disintegrants, pH regulators and solubilizers.

5. The method according to claim 4, wherein: The dosage form of the finished composition includes but is not limited to solid beverages, oral liquids, compressed candies, soft capsules, and the like.

6. The method according to claim 3, wherein: The steps for preparing the pig brain protein hydrolysate in S1 are as follows: S1. First, the fresh pig brain is manually removed of impurities, and then the brain is pulverized into 2-3 cm tissue blocks using a block cutter, and then the pulverized tissue blocks are cleaned with water, and then placed in a cooking tank and water is added for cooking, wherein the volume ratio of the tissue block to water is 1:2-1:5, the cooking temperature is 80-120°C, the cooking pressure is 1-3MPa, and the cooking time is 2-6 hours to obtain a cooking liquid; S2. The cooking liquid obtained in S1 is separated into oil and water by gravity separation, and the resulting aqueous phase is cooled to 50 to 55° C. to obtain a crude collagen solution; S3. Add the composite enzyme preparation to the crude collagen solution obtained in S2, mix well and perform enzymolysis for 0.5-3 hours to obtain a collagen peptide solution, wherein the composite enzyme preparation is composed of trypsin, chymotrypsin and alkaline protease; S4. Filtering the collagen peptide solution obtained in S3 to remove tissue fragments and other impurities, and then filtering with an inorganic nanofiltration ceramic membrane with a molecular weight cutoff of 800Da to further obtain purified collagen peptides; S5. Concentrate the purified collagen peptides obtained in S4, spray-dry, and obtain the final porcine brain protein hydrolysate.

7. The method according to claim 3, wherein: The preparation steps of the mixed extract of Gastrodia elata, American ginseng, Ginkgo biloba, Cornus officinalis, Polygonatum sibiricum, Morus alba, Pueraria lobata and Asparagus cochinchinensis in S3 are as follows: S1. Cut the dried Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaves, kudzu root and asparagus into small pieces of 2 to 3 mm, and the cutting is completed by a knife or a cutting machine; S2. The cut Gastrodia elata, American ginseng, ginkgo, cornus officinalis, polygonatum, mulberry leaf, kudzu root and asparagus pieces are added to a closed extraction tank according to the ratio of claim 1, and purified water is added according to a mass volume ratio of 1:1-1:10, extracted at 70-130 ° C for 1-8 hours, filtered to obtain the supernatant, and the extraction is repeated 2-5 times; S3. Collect all the supernatant and concentrate it to obtain "plant concentrate", which is then placed in a container for later use.

8. The method according to any one of claims 3 to 7, wherein: The composition is a food composition.

9. Use of the composition of claim 1 or the composition prepared by the method of any one of claims 3 to 7 in the preparation of a medicament for treating and preventing Alzheimer's disease.

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