Extraction method of radish cream
By crushing the radish and Glauber's salt and mixing it with water, and placing the precipitated frost in a specific environment, the radish frost was successfully extracted, solving the problem of unsuccessful weathering and frost making in the prior art, and the extraction of radish frost and its therapeutic effects were achieved.
Patent Information
- Application Number
- CN202411232518.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-04
- Publication Date
- 2025-04-11
AI Technical Summary
Vegetables that have not yet been successfully weathered and frosted in the prior art include radish, which has resulted in the extraction method of radish frost not being studied and applied.
The method of collecting the radish and Glauber's salt after crushing it with water, mixing it with water, putting it at an environment of 0°C to 20°C, and a relative humidity of 1% to 60%, and collecting the frost after precipitation is achieved to achieve the extraction of the radish frost.
This method was successfully extracted, which has the effects of reducing swelling and relieving pain, clearing heat and detoxifying. It is suitable for treating symptoms such as swelling and pain in the throat, sores in the mouth and tongue, and swollen and painful gums. It also has a deswelling and anti-it-it-out effect on edema and itching caused by mosquito bites.
Smart Images
Figure CN119792371A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicine processing, and in particular relates to an extraction method of radish frost. Background Art
[0002] Chinese medicine processing is a traditional pharmaceutical technology for preparing Chinese medicine slices. Through processing, the medicine can be made into loose powder or precipitated tiny crystals. Their appearance is similar to frost, so they are called "frost". This processing method is called frost making. The purpose of drug frost making is to reduce toxicity, alleviate drug efficacy, eliminate side effects, and improve efficacy to achieve the best therapeutic effect. The specific method of frost making is to sprinkle certain salt drugs on the melon pulp with skin, put it into an earthen jar, cover it and seal it, place it in a cool and ventilated place, let the precipitated crystals condense outside the earthen jar, and sweep it down with a brush until there are no crystals outside the jar.
[0003] The study of effective ingredients of frost-type Chinese medicine has always been highly valued. Li Yanfeng et al. believed that microbial fermentation is the connotation of frost-type Chinese medicine processing. They found a large number of hyphae in watermelon frost. The antibacterial activity of watermelon frost extract was studied and found that it has strong antibacterial and bactericidal activity against oral pathogens and common pathogens. Then, the fermentation microorganisms in the traditional processing process of watermelon frost were separated and identified. Six fungi were isolated from the traditional processing process of watermelon frost, and their colors were white, yellow, green and gray, respectively, which was consistent with the color changes of watermelon frost during the processing under natural conditions. These six fungi were identified as Fusarium, Penicillium, Alternaria and Mucor4. This shows that there is indeed a microbial fermentation process in the processing of watermelon frost, and some high-salt-tolerant microorganisms and their secondary metabolites involved in the fermentation may have a huge impact on the material basis of the efficacy of watermelon frost.
[0004] Modern medical research shows that radish contains high levels of glucosinolates and rich varieties, among which 4-methylthio-3-butenyl glucosinolate has the highest content, accounting for more than 90% of the total glucosinolate content, and has antibacterial, anti-inflammatory, antioxidant, and anti-tumor activities.
[0005] Glucosinolates will degrade to produce sulforaphene (4-methylsulfinyl-3-butenyl isothiocyanate) under the conditions of myrosinase or high temperature and high pressure, which has antioxidant, anti-cell mutation, anti-cancer and plant antibacterial and herbicidal effects. Among them, isothiocyanates (sulforaphane, isothiocyanophenethyl ester, etc.) have strong anticancer activity. However, sulforaphane is unstable in nature, difficult to produce and prepare, and has a low synthesis rate, which is not suitable for industrial production. However, obtaining it through the degradation of glucosinolates can greatly improve productivity. Studies have shown that during the fermentation of vegetables, glucosinolates are degraded to produce active metabolites such as isothiocyanates, nitriles, and cyclic nitriles. A study by Yang Xiaoming et al. in 2014 showed that the edible solid taproot of Asian white radish has anti-inflammatory and anti-cancer effects, and sulforaphane found in radish can inhibit the proliferation of cervical cancer cell line HeLa cells. At present, cucumbers and watermelons have been successfully weathered and frosted. Therefore, in view of the unique medicinal effects of radish, an extraction of radish frost is urgently needed. Summary of the invention
[0006] The purpose of the present invention is to solve the shortcomings of the prior art, and to propose an extraction method of radish frost, which solves the problem that only cucumbers and watermelons have been successfully weathered and frosted in the prior art, but not radishes.
[0007] In order to achieve the above object, the present invention adopts the following technical scheme: a radish frost extraction and method thereof, comprising at least the following steps:
[0008] S1: Grind the radish and the sodium sulfate respectively, add water and mix them evenly to obtain a mixture;
[0009] S2: placing the mixture obtained in S1 in an environment of 0°C to 20°C and a relative humidity of 1% to 60%;
[0010] S3: After frost is precipitated in S2, collect the frost until the frost precipitation stops.
[0011] The radish described in S1 is taken as 100 g; the sodium sulfate is taken as 10 g to 30 g; and the water is taken as 20 mL to 40 mL.
[0012] In S1, the radish and the sodium sulfate are crushed and filtered respectively using a 30-50 mesh filter.
[0013] The mixture obtained in S1 is first placed in a container, and then placed in an environment of 0°C to 20°C and a relative humidity of 1 to 60%, wherein the container is a container that is not corroded by Glauber's salt.
[0014] Select radishes with a water content of 60% to 80%.
[0015] The container is made by hollowing out the core of a radish, removing the flesh and leaving the skin.
[0016] The collection method in S3 is: use a brush to brush off the frost precipitated on the surface of the container and collect it.
[0017] The radish is white radish.
[0018] The present invention has the following beneficial effects:
[0019] 1. The present invention is the first to study the process of preparing radish frost by using Glauber's salt, which provides a basis for the production of glucosinolates and sulforaphane in radish, the clinical research significance and the establishment of radish frost quality standards. Radish frost tastes salty. According to the comparison of the frost making process with the research results of watermelon frost, it is predicted that the main component is recrystallized Na2SO4·10H2O, which is verified and obtained through experiments. It is inferred that they have similar effects, both of which have the effects of reducing swelling and relieving pain, clearing away heat and detoxifying. Radish can help digestion and resolve phlegm, and Glauber's salt can clear away heat and purge fire. The two drugs are made in a synergistic way, which makes the drugs purer and enhances the effects of reducing swelling and relieving pain, clearing away heat and detoxifying. Radish frost is used for sore throat, mouth sores, swollen and painful gums, acute and chronic pharyngitis, tonsillitis, stomatitis, and oral ulcers.
[0020] 2. The 5-10% radish frost solution of the present invention has a good effect of reducing swelling and relieving itching on edema and itching caused by mosquito bites.
[0021] 3. The research on the frosting process of radish and salt is based on the traditional frosting process. According to the water content of radish, a certain proportion of water is added in the frosting process to ensure complete dissolution and fermentation. Radish frosting is made by processing radish and mirabilite. It has a laxative effect, can increase the water content in the intestine, cause mechanical stimulation, and promote intestinal peristalsis. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 A flowchart of a radish frost extraction method and a method thereof provided by the present invention;
[0023] Figure 2 The effect of temperature on the amount of frost produced by radish;
[0024] Figure 3 The effect of relative humidity on the amount of frost produced by radish;
[0025] Figure 4 The effect of drug-material ratio on frost yield of radish;
[0026] Figure 5 To study the effect of processing time on the amount of frost produced by radish; Figure 6 The effect of the amount of water added on the amount of frost produced by radish. DETAILED DESCRIPTION
[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0028] Example 1
[0029] A method for extracting radish frost and the method at least comprise the following steps:
[0030] S1: Take 100 g of radish with a water content of 60% and wash it clean;
[0031] S2: chop the radish, crush it into 30 meshes according to the ratio of radish: sodium sulfate: water = 100 g: 10 g: 20 mL, stir it evenly to obtain a mixture, and put the mixture into a container;
[0032] S3: Place the container in S2 in an environment with a temperature of 0°C and a relative humidity of 1%;
[0033] S4: After radish frost is continuously precipitated outside the container in S3, the frost is brushed off and collected gradually until there is no frost.
[0034] Example 2
[0035] A method for extracting radish frost and the method at least comprise the following steps:
[0036] S1: Take 100 g of radish with a water content of 70% and wash it clean;
[0037] S2: Take out the pulp of the radish and keep the skin to obtain a radish skin container, chop the pulp, weigh the mirabilite and grind it into 40 meshes, add water, mix the pulp, mirabilite and water, pour them into the container, seal it, and obtain a radish skin mixture. According to radish: mirabilite: water = 100g: 15g: 30mL;
[0038] S3: placing the radish peel mixture in S2 in an environment with a temperature of 10°C and a relative humidity of 30%;
[0039] S4: After radish cream is continuously precipitated out of the radish peel mixture in S3, brush it off and collect it until there is no frost.
[0040] Example 3
[0041] A method for extracting radish frost and the method at least comprise the following steps:
[0042] S1: Take 100 g of radish with a water content of 80% and wash it clean;
[0043] S2: chop the radish, crush it into 50 meshes according to the ratio of radish: sodium sulfate: water = 100 g: 30 g: 40 mL, stir it evenly to obtain a mixture, and put the mixture into a container;
[0044] S3: Place the container in S2 in an environment with a temperature of 20°C and a relative humidity of 60%;
[0045] S4: After radish frost is continuously precipitated outside the container in S3, brush it off and collect it until there is no frost.
[0046] Because the radish cream prepared in Examples 1 and 3 is the same as that prepared in Example 2, the radish cream prepared in Examples 1 and 3 also has the functions of reducing swelling and relieving pain, clearing away heat and detoxifying, and can be used for sore throat, mouth sores, sore gums, acute and chronic pharyngitis, tonsillitis, stomatitis, and oral ulcers.
[0047] The following test takes the method of Example 2 as an example.
[0048] Effect of temperature on frost yield of radish
[0049] The temperature was set at 0℃, 5℃, 10℃, 15℃, and 20℃, the ratio of radish to mirabilite was 100:15, the amount of water added was 30mL, the preparation time was 6 days, and the amount of frost was as follows: Figure 2 As shown in the figure, it can be seen that the frost output is highest when the temperature is 15℃.
[0050] Single factor experiment
[0051] Effect of relative humidity on frost yield of radish
[0052] Referring to the preparation method of Example 2, the relative humidity was set to 10%, 20%, 30%, 40%, and 50%, respectively, the drug ratio of radish to mirabilite was 100:15, the amount of water added was 30 mL, the processing time was 6 days, and the amount of frost was as follows: Figure 3 As shown in the figure, it can be seen that the frost output is highest when the relative humidity is 40%.
[0053] Effect of drug-drug ratio on frost yield of radish
[0054] Referring to the preparation method of Example 2, the amount of water added was 30 mL, the processing time was 6 days, and the frost amount of radish was observed when the drug ratio of radish to mirabilite was 90:15, 100:15, 110:15, and 120:15 respectively. Figure 4 As shown in the figure, it can be seen that when the ratio of radish to Glauber's salt is 100:15, the frost output is the highest.
[0055] Effect of processing time on frost yield of radish
[0056] Referring to the preparation method of Example 2, the amount of water added was 30 mL, the ratio of radish to mirabilite was 100:15, and the amount of frost on the 3rd, 4th, 5th, and 6th days of preparation was observed. Figure 5 As shown in the figure, it can be seen that the frost output is the highest when the processing time is 5 days.
[0057] Effect of water addition on frost production of radish
[0058] Referring to the preparation method of Example 2, the ratio of radish to mirabilite was 100:15, the preparation time was 6 days, and the frost amount when the amount of water added was 10mL, 20mL, 30mL, and 40mL was observed. Figure 6 As shown in the figure, it can be seen that when the amount of water added is 20mL, the frost output is the highest.
[0059] Determination of frost yield of radish frost
[0060] Radish frost was collected every day, placed in a constant weight beaker and placed in a desiccator. After collection, it was dried for 24 hours and weighed using a balance with an accuracy of 0.1 mg.
[0061] Determination of sodium sulfate content
[0062] Take 0.2g of sample, add 150mL of water, shake for 10 minutes, filter, wash the precipitate with 50mL of water three times, filter, combine the filtrate, add 1mL of hydrochloric acid, boil, stir constantly, and slowly add 20mL of 60℃, 1.2% barium chloride test solution until no more precipitate is generated, heat on a water bath for 30min, let stand for 1h, filter with ash-free filter paper or a weighed Gooch crucible, wash the precipitate with water several times until the washing solution no longer shows chloride reaction, dry, and ignite to constant weight, accurately weigh, and multiply by 0.6086 to obtain the weight of sodium sulfate (Na2SO4) contained in the test sample.
[0063] Experimental factor level table
[0064] Table 1 Experimental factor level table
[0065]
[0066] Weighted comprehensive scoring analysis
[0067] Table 2 Test results analysis
[0068]
[0069]
[0070] Note: “-” means no result.
[0071] From the table above, we can see that the experimental result of group 9 experiment A2B2C2 is the best, which is the optimal level combination obtained from the experiment. The K value results in Table 2 show that the index value level K2 in factor A is the largest value among the KiA values; the index value level K2B in factor B is the largest value among the KiB values; the index value level K2C in factor C is the largest value among the KiC values; therefore, in theory, the optimal level combination for radish weathering into frost is A2B2C2. That is, the optimal level combination is the radish-salt medicine ratio (100:15), the processing time is 5 days, and the amount of water added is 30mL.
[0072] Data analysis
[0073] Weighted comprehensive analysis of frost rate of radish and sodium sulfate content in radish frost
[0074] Table 3 Analysis results
[0075] source Factor sum of squares Degrees of Freedom Mean Square F-number P A 0.714 2 357 61.640 P＜0.05 B 0.234 2 0.117 20.238 P＜0.05 C 0.006 2 0.003 0.517 P＞0.05 error 0.012 2 0.006 - -
[0076] Note: F0.05(2,2)=19.00, “-” means no result.
[0077] From the analysis of Table 3, we can see that the influence of factors A, B, and C on the radish frosting process is drug-material ratio>processing time>water addition. Factors A (drug-material ratio) and B (processing time) have significant effects on the frosting process, while C (water addition) has no significant effect on the radish frosting process.
[0078] Confirmatory experiments
[0079] According to the above results, the optimal process for making frost from radish is: the drug-material ratio of radish to Glauber's salt is 100:15, the processing days are 5 days, the amount of water added is 30 mL, the optimal frost output is 3.7913 g, and the sodium sulfate content is 91.8%. In order to verify the reliability of the optimal process, the optimal process conditions were used for verification, with a drug-material ratio of 100:15, a processing days of 5 days, and a water addition amount of 30 mL. Three parallel experiments were conducted, and the experimental results are shown in Table 4.
[0080] Table 4. Processing verification results of the optimal level combination
[0081] Experiment No. 1 2 3 average value Radish cream weight (g) 3.7806 3.7580 3.7643 3.7676 Frost rate (%) 3.2875 3.2678 3.2733 3.2762 Sodium sulfate content (%) 90.2523 90.9845 91.6615 90.6661
[0082] From Table 4, it can be seen that the average frost amount of radish is 3.7676g, the average frost rate is 3.2762%, the average sodium sulfate content is 90.6661%, and the relative standard deviation RSD values are 0.31%, 0.31% and 0.78%, respectively. The experimental error is small.
[0083] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.
Claims
1. A method for extracting radish frost and the method comprising at least the following steps: S1: Grind the radish and the sodium sulfate respectively, add water and mix them evenly to obtain a mixture; S2: placing the mixture obtained in S1 in an environment of 0°C to 20°C and a relative humidity of 1% to 60%; S3: After frost is precipitated in S2, collect the frost until the frost precipitation stops.
2. The method for extracting radish frost according to claim 1, characterized in that: The radish described in S1 is taken as 100 g; the sodium sulfate is taken as 10 g to 30 g; and the water is taken as 20 mL to 40 mL.
3. The extraction method of radish frost according to claim 1, characterized in that: In S1, the radish and the sodium sulfate are crushed and filtered respectively using a 30-50 mesh filter.
4. The extraction method of radish frost according to claim 1, characterized in that: The mixture obtained in S1 is first placed in a container, and then placed in an environment of 0°C to 20°C and a relative humidity of 1% to 60%, wherein the container is a container that is not corroded by Glauber's salt.
5. The extraction method of radish frost according to claim 1, characterized in that: Select radishes with a water content of 60% to 80%.
6. The extraction method of radish frost according to claim 1, characterized in that: The container is made by hollowing out the heart of a radish.
7. The extraction method of radish frost according to claim 1, characterized in that: The collection method in S3 is: use a brush to brush off the frost precipitated on the surface of the container and collect it.
8. The extraction method of radish frost according to claim 1, characterized in that: The radish is white radish.