Restoration material for simultaneously treating sediment and water bodies, preparation method and application thereof

Abstract

This application relates to the field of water pollution control technology, specifically to remediation materials that simultaneously treat sediment and water, their preparation methods, and applications. The method for preparing the remediation material includes: adding Al-MOFs and mixed activated carbon powder to an organic solvent, shaking, cooling, filtering, washing, and drying to obtain porous activated carbon material; adding the porous activated carbon material to a calcium lactate solution and stirring, then adding sodium alginate solution for forward spheroidization, followed by filtering, washing, and drying to obtain porous activated carbon material gel spheres; adding the porous activated carbon material gel spheres and a composite bacterial agent to water, shaking and culturing in a shaker, then filtering, washing, and drying to obtain bacteria-containing porous material; adding the bacteria-containing porous material to a sodium alginate solution, stirring, and adding calcium lactate solution dropwise for reverse spheroidization, followed by filtering, washing, and drying to obtain the remediation material. This remediation material possesses excellent water and sediment remediation effects without burdening the aquatic ecosystem.

Description

Technical Field

[0001] This application relates to the field of water pollution control technology, specifically to remediation materials that simultaneously treat sediment and water, their preparation methods, and applications. Background Technology

[0002] Previously, due to the expansion of urbanization, the increase in urban population, inadequate water pollution control measures, and outdated treatment equipment, the amount of industrial, agricultural, and domestic wastewater in urban water bodies has been continuously increasing. This has led to organic pollution in the water environment exceeding its self-purification capacity. Simultaneously, due to insufficient hydrodynamics and poor water flow, pollutants have been continuously accumulating in the bottom sediment. The sediment and the overlying water body constantly migrate and transfer pollutants, ultimately forming black and odorous water bodies, which have seriously impacted the urban landscape, the ecological environment, and residents' health. In recent years, with increased environmental awareness and strengthened environmental supervision, the discharge of external pollution sources such as industrial, agricultural, and domestic wastewater has been controlled. However, bottom sediment, as an internal source of pollution, remains a major source of pollution.

[0003] For sediment remediation, various technologies have been developed, including physical remediation, chemical remediation, and bioremediation. While physical technologies are low-cost and effective, they do not fundamentally address endogenous pollution and are easily disturbed and eroded by water bodies. Chemical remediation is fast-acting and effective, but it can harm aquatic life. Bioremediation is less harmful, but it is time-consuming and easily affected by temperature. To achieve better sediment remediation, methods combining multiple technologies have been developed. For example, existing technology CN112441713B provides an in-situ remediation composite material and a method for in-situ remediation of contaminated sediment. However, its specific technical solution involves separately preparing chemical and bioremediation materials and using them sequentially. The preparation and application steps are cumbersome, and the remediation agents contain a large amount of cement during preparation. This can easily lead to the sediment layer in the water being covered by cement, making it difficult for plants, microorganisms, and fish with water purification functions to grow, thus hindering the restoration of the water body's natural purification capacity.

[0004] Therefore, a new remediation material is needed that can restore water bodies and sediment without affecting the water's own purification capacity. Summary of the Invention

[0005] The problem this application aims to solve is to provide remediation materials that simultaneously treat sediment and water, as well as their preparation methods and applications, which have excellent water and sediment remediation effects without burdening the aquatic ecological environment.

[0006] To solve the above-mentioned technical problems, this application adopts the following technical solution:

[0007] On the one hand, this application provides a method for preparing remediation materials that simultaneously treat sediment and water, comprising the following steps:

[0008] Al-MOFs and mixed activated carbon powder were dispersed in an organic solvent, shaken, cooled to room temperature, and then subjected to a first filtration, a first washing, and a first drying to obtain aluminum-based MOF porous activated carbon material.

[0009] The aluminum-based MOF porous activated carbon material is added to a first calcium lactate solution and stirred for the first time. During the first stirring, a first sodium alginate solution is added. After positive spheroidization, the material is filtered, washed, and dried to obtain aluminum-based MOF porous activated carbon material gel spheres.

[0010] The aluminum-based MOF porous activated carbon material gel balls and composite bacterial agent were added to water and placed in a shaker for oscillation culture. Then, a third filtration, a third washing, and a third drying were performed to obtain a porous material containing bacteria.

[0011] The bacterial porous material is added to a second sodium alginate solution and stirred for the second time. During the second stirring process, a second calcium lactate solution is added dropwise. After reverse spheroidization, the material undergoes a fourth filtration, a fourth washing, and a fourth drying to obtain the remediation material that simultaneously treats sediment and water.

[0012] Furthermore, the Al-MOFs include one of MIL-53(Al) and CAU-1.

[0013] Furthermore, the mixed activated carbon powder includes wood-based activated carbon powder, coal-based activated carbon powder, and fruit shell activated carbon powder.

[0014] Furthermore, the compound microbial agent includes photosynthetic bacteria, Bacillus, phosphorus-fixing bacteria, denitrifying bacteria, and nitrate-reducing bacteria.

[0015] Furthermore, the weight ratio of the Al-MOFs, the activated carbon powder, and the composite microbial agent is 2:(2-4):(1-2).

[0016] Furthermore, the weight ratio of the wood-based activated carbon powder, the coal-based activated carbon powder, and the fruit shell activated carbon powder is (10-20):(5-15):(3-7).

[0017] Furthermore, the weight ratio of the photosynthetic bacteria, the Bacillus, the phosphorus-fixing bacteria, the denitrifying bacteria, and the nitrate-reducing bacteria is (5-9):(8-16):(9-21):(3-7):(2-6).

[0018] Furthermore, the concentrations of the first sodium alginate solution and the second sodium alginate solution are 1.0–2.0 wt%.

[0019] Furthermore, the concentrations of the first calcium lactate solution and the second calcium lactate solution are 2.0–3.0 wt%.

[0020] Furthermore, the organic solvent includes one of ethanol and dimethylformamide.

[0021] Furthermore, the water includes ddH2O.

[0022] Furthermore, the oscillation method includes ultrasonic oscillation, the oscillation temperature is 160-180°C, and the oscillation time is 24-36 hours.

[0023] Furthermore, the temperature of the oscillation culture is 35–37°C, the oscillation culture speed is 160–180 rpm, and the oscillation culture time is 48–72 h.

[0024] On the other hand, this application provides a remediation material that simultaneously treats sediment and water, prepared by the above method.

[0025] Furthermore, this application provides for the application of the aforementioned methods or repair materials.

[0026] Furthermore, the applications include one of the following: water body remediation, sediment remediation, and simultaneous remediation of water body and sediment.

[0027] This application has the following beneficial effects:

[0028] 1. The remediation material provided in this application, which simultaneously treats sediment and water, uses Al-MOFs as a carrier material to provide adsorption sites for the mixed activated carbon powder. By organically combining the porous adsorption material with the activated carbon material, the remediation material's ability to clean up pollutants such as organic and inorganic matter in water and sediment is greatly improved.

[0029] 2. The remediation material for simultaneously treating sediment and water provided in this application utilizes forward spheroidization to form an ultra-thin and tough gel isolation membrane on the surface of aluminum-based MOF porous activated carbon material during the preparation process. This membrane can separate the composite bacterial agent from the aluminum-based MOF porous activated carbon material, thus preventing the porous adsorption material from affecting the remediation ability of the composite bacterial agent.

[0030] 3. The remediation material provided in this application, which simultaneously treats sediment and water, incorporates composite microbial agents and aluminum-based MOF porous activated carbon within a thicker gel outer membrane formed by reverse spheroidization during the preparation process, thereby improving the storage stability of the remediation material.

[0031] 4. The remediation material provided in this application, which simultaneously treats sediment and water, can, under the influence of water movement, cause the outer gel membrane of the sediment to rupture through friction between the hard components and the gel membrane, thereby releasing the internal compound microbial agent.

[0032] 5. The remediation material provided in this application, which simultaneously treats sediment and water, is made of biocompatible materials such as Al-MOFs, activated carbon powder, and sodium alginate gel. It not only has excellent sediment and water remediation capabilities in the early stages of use, but also has the potential to provide growth and attachment space for the restoration of aquatic biological communities. Detailed Implementation

[0033] The technical solutions in some embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments provided in this application, all other embodiments obtained by those skilled in the art are within the scope of protection of this application.

[0034] Example 1

[0035] Prepare the mixed activated carbon powder according to the following steps:

[0036] S11. Add 10 parts of wood-based activated carbon granules, 5 parts of coal-based activated carbon granules and 3 parts of fruit shell activated carbon granules to a grinder and grind for 1 hour. Then, sieve the mixture through a 325-mesh sieve and collect the sieved powder to obtain the mixed activated carbon powder.

[0037] Example 2

[0038] Prepare the mixed activated carbon powder according to the following steps:

[0039] S11. Add 15 parts of wood-based activated carbon granules, 10 parts of coal-based activated carbon granules and 5 parts of fruit shell activated carbon granules to a grinder and grind for 1 hour. Then, sieve the mixture through a 325-mesh sieve and collect the sieved powder to obtain the mixed activated carbon powder.

[0040] Example 3

[0041] Prepare the mixed activated carbon powder according to the following steps:

[0042] S11. Add 20 parts of wood-based activated carbon granules, 15 parts of coal-based activated carbon granules and 7 parts of fruit shell activated carbon granules to a grinder and grind for 1 hour. Then, sieve the mixture through a 325-mesh sieve and collect the sieved powder to obtain the mixed activated carbon powder.

[0043] Example 4

[0044] Prepare the compound microbial agent according to the following steps:

[0045] S21. Add 5 parts photosynthetic bacteria, 8 parts Bacillus, 9 parts phosphorus-fixing bacteria, 3 parts denitrifying bacteria and 2 parts nitrate-reducing bacteria to 50 parts deionized water, sonicate for 2 hours, and then freeze-dry to obtain the compound bacterial agent.

[0046] Example 5

[0047] Prepare the compound microbial agent according to the following steps:

[0048] S21. Add 7 parts photosynthetic bacteria, 12 parts Bacillus, 15 parts phosphorus-fixing bacteria, 5 parts denitrifying bacteria and 4 parts nitrate-reducing bacteria to 50 parts deionized water, sonicate for 2 hours, and then freeze-dry to obtain the compound bacterial agent.

[0049] Example 6

[0050] Prepare the compound microbial agent according to the following steps:

[0051] S21. Add 9 parts of photosynthetic bacteria, 16 parts of Bacillus, 21 parts of phosphorus-fixing bacteria, 7 parts of denitrifying bacteria and 6 parts of nitrate-reducing bacteria to 50 parts of deionized water, sonicate for 2 hours, and then freeze-dry to obtain the compound bacterial agent.

[0052] Example 7

[0053] Prepare a remediation material that simultaneously treats sediment and water according to the following steps:

[0054] S31. Disperse 20 parts of MIL-53(Al) and 20 parts of the mixed activated carbon powder prepared in Example 1 in 100 parts of ethanol, ultrasonically vibrate at 160°C for 24 hours, cool to room temperature, filter, wash three times with anhydrous ethanol and air dry to obtain aluminum-based MOF porous activated carbon material.

[0055] S32. Add the aluminum-based MOF porous activated carbon material to 300 parts of 2.0wt% calcium lactate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 120 parts of 1.0wt% sodium alginate solution. After positive spheroidization, filter the mixture, wash it three times with ddH2O, and air dry the surface moisture to obtain aluminum-based MOF porous activated carbon material gel spheres.

[0056] S33. Add the aluminum-based MOF porous activated carbon material gel balls and 10 parts of the composite bacterial agent prepared in Example 4 to 200 parts of ddH2O, and place them in a shaker at 35°C and 180 rpm for 48 h. After filtration, wash three times with ddH2O and air dry the surface moisture to obtain the bacterial porous material.

[0057] S34. Add the bacterial porous material to 100 parts of 1.0wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 300 parts of 2.0wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the remediation material that simultaneously treats sediment and water.

[0058] Example 8

[0059] Prepare a remediation material that simultaneously treats sediment and water according to the following steps:

[0060] S31. Disperse 20 parts of MIL-53(Al) and 30 parts of the mixed activated carbon powder prepared in Example 2 in 100 parts of ethanol, ultrasonically vibrate at 170°C for 30 hours, cool to room temperature, filter, wash three times with anhydrous ethanol and air dry to obtain aluminum-based MOF porous activated carbon material.

[0061] S32. Add the aluminum-based MOF porous activated carbon material to 300 parts of 2.5wt% calcium lactate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 100 parts of 1.5wt% sodium alginate solution. After positive spheroidization, filter the mixture, wash it three times with ddH2O, and air dry the surface moisture to obtain aluminum-based MOF porous activated carbon material gel spheres.

[0062] S33. Add the aluminum-based MOF porous activated carbon material gel balls and 15 parts of the composite bacterial agent prepared in Example 5 to 200 parts of ddH2O, and place them in a shaker at 36°C and 170 rpm for 36 h of shaking culture. After filtration, wash three times with ddH2O and air dry the surface moisture to obtain the bacterial porous material.

[0063] S34. Add the bacterial porous material to 120 parts of 1.5wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 300 parts of 2.5wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the remediation material that simultaneously treats sediment and water.

[0064] Example 9

[0065] Prepare a remediation material that simultaneously treats sediment and water according to the following steps:

[0066] S31. Disperse 20 parts of CAU-1 and 40 parts of the mixed activated carbon powder prepared in Example 3 in 100 parts of dimethylformamide, ultrasonically vibrate at 180°C for 36 hours, cool to room temperature, filter, wash three times with dimethylformamide and air dry to obtain aluminum-based MOF porous activated carbon material.

[0067] S32. The aluminum-based MOF porous activated carbon material is added to 320 parts of 3.0wt% calcium lactate solution and the magnetic stirrer is turned on. During the magnetic stirring process, 100 parts of 2.0wt% sodium alginate solution are added. After positive spheroidization, the mixture is filtered, then washed three times with ddH2O and the surface moisture is dried to obtain aluminum-based MOF porous activated carbon material gel spheres.

[0068] S33. Add the aluminum-based MOF porous activated carbon material gel balls and 20 parts of the composite bacterial agent prepared in Example 6 to 200 parts of ddH2O, and place them in a shaker at 160 rpm at 37°C for 72 h of shaking culture. After filtration, wash three times with ddH2O and air dry the surface moisture to obtain the bacterial porous material.

[0069] S34. Add the bacterial porous material to 100 parts of 2.0wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 320 parts of 3.0wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the remediation material that simultaneously treats sediment and water.

[0070] Comparative Example 1

[0071] Prepare the repair material according to the following steps:

[0072] The method for preparing the repair material in this comparative example is the same as in Example 7; the only difference between Comparative Example 1 and Example 7 is that:

[0073] In S31, Al-MOFs are replaced with zeolites.

[0074] Comparative Example 2

[0075] Prepare the repair material according to the following steps:

[0076] S41. Add 20 parts of MIL-53(Al) to 300 parts of 2.0wt% calcium lactate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 120 parts of 1.0wt% sodium alginate solution. After positive spheroidization, filter the solution, wash it three times with ddH2O and air dry the surface moisture to obtain aluminum-based MOF porous activated carbon material gel spheres.

[0077] S42. Add the aluminum-based MOF porous activated carbon material gel balls and 10 parts of the composite bacterial agent prepared in Example 4 to 200 parts of ddH2O, and place them in a shaker at 35°C and 180 rpm for 48 h. After filtration, wash three times with ddH2O and air dry the surface moisture to obtain the bacterial porous material.

[0078] S43. Add the bacterial porous material to 100 parts of 1.0wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 300 parts of 2.0wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the repair material.

[0079] Comparative Example 3

[0080] Prepare the repair material according to the following steps:

[0081] S51. Disperse 20 parts of MIL-53(Al) and 20 parts of the mixed activated carbon powder prepared in Example 1 in 100 parts of ethanol, ultrasonically vibrate at 160°C for 24 hours, cool to room temperature, filter, wash three times with anhydrous ethanol and air dry to obtain aluminum-based MOF porous activated carbon material.

[0082] S52. The aluminum-based MOF porous activated carbon material and 10 parts of the composite bacterial agent prepared in Example 4 were added to 200 parts of ddH2O, and then placed in a shaker at 180 rpm at 35°C for 48 hours. After filtration, the material was washed three times with ddH2O and the surface moisture was dried to obtain the bacterial porous material.

[0083] S53. Add the bacterial porous material to 100 parts of 1.0wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 300 parts of 2.0wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the repair material.

[0084] Comparative Example 4

[0085] Prepare the repair material according to the following steps:

[0086] S61. Add 10 parts of the composite bacterial agent prepared in Example 4 to 200 parts of ddH2O, and place it in a shaker at 180 rpm at 35°C for 48 hours. After filtration, wash it three times with ddH2O and air dry the surface moisture to obtain a porous material containing bacteria.

[0087] S62. Add the bacterial porous material to 100 parts of 1.0wt% sodium alginate solution and turn on the magnetic stirrer. During the magnetic stirring process, add 300 parts of 2.0wt% calcium lactate solution dropwise. After reverse spheroidization, filter, wash three times with ddH2O, and air dry the surface moisture to obtain the repair material.

[0088] Experiment 1 verifies the ammonia nitrogen removal effect of remediation materials that simultaneously treat sediment and water.

[0089] Take 1g of the remediation material prepared in Examples 7-9 and Comparative Examples 1-4 respectively, and add it to 100mL of ammonia nitrogen solution or methylene blue solution with a concentration of 100mg / L. The pH of the ammonia nitrogen solution and methylene blue solution should be adjusted to 7 beforehand using hydrochloric acid or sodium hydroxide. Then, place the ammonia nitrogen solution or methylene blue solution in a constant temperature shaker at 25℃ and shake at a speed of 200r / min for 150min for adsorption. After adsorption is complete, the removal rate of ammonia nitrogen compounds is measured and calculated using Nessler's reagent method, or the removal rate of methylene blue is measured and calculated using methylene blue spectrophotometry. The results are shown in Table 1.

[0090] Table 1:

[0091]

[0092]

[0093] As shown in Table 1, the remediation material of this application, which simultaneously treats sediment and water, has excellent removal effects on both ammonia nitrogen compounds and methylene blue. Among them, Example 9 has the best removal effect, achieving a 43.1% ammonia nitrogen removal rate and a 39.8% methylene blue removal rate in just 150 minutes. In other words, the remediation material of this application has excellent water treatment effect.

[0094] Experiment Example 2: Simulation Experiment of River Treatment Using Remediation Materials that Simultaneously Treat Sediment and Water

[0095] River water and surface sediment from a secondary river in a city in Sichuan Province were collected and tested in a river simulation device. The reactor measures 5m × 1m × 0.5m and is made of transparent tempered glass. The simulated sediment thickness is 15cm, and the simulated water thickness is 20cm. A 20cm baffle is installed at the downstream position of the reactor to prevent the sediment and remediation materials from being washed away. The reactor is equipped with a water circulation system, a water movement simulation system, an energy dissipation plate, and a laminar flow diversion pipe to simulate the real river conditions. At the same time, an aeration device is used to continuously aerate the river water. Two sampling ports are set at the downstream position of the reactor, at heights of 13cm and 25cm respectively, and the sampling ports are numbered 1 and 2 from bottom to top. 65g of the remediation materials prepared in Examples 7-9 and Comparative Examples 1-4 were added to the reactor, respectively. Samples were taken at two sampling ports at 0h, 48h and 96h after the addition of the remediation materials. The total organic carbon (TOC), total phosphorus (TP) and total phosphorus (TN) contents at the two sampling ports were detected. The test results are shown in Table 2.

[0096] Table 2:

[0097]

[0098] As shown in Table 2, the remediation material provided in this application can produce significant remediation effects on both water and sediment, and the remediation effect is very rapid, completing the treatment of most pollutants in the water and sediment in just three days.

[0099] In summary, the remediation material provided in this application, which simultaneously treats sediment and water, uses biocompatible Al-MOFs and activated carbon powder as raw materials to prepare an aluminum-based MOF porous activated carbon material. Sodium alginate gel is used to encapsulate the composite bacterial agent with the aluminum-based MOF porous activated carbon material in a non-contact manner, avoiding interference from the aluminum-based MOF porous activated carbon material with the release of the composite bacterial agent. This remediation material can rapidly treat inorganic and organic pollutants in the water and possesses excellent cleaning capabilities. One of the raw materials in this remediation material is sodium alginate gel. As a biodegradable coating material, sodium alginate gel exposes the internal porous material in the sediment after degradation. The porous material, being biocompatible, also has the potential to provide growth and attachment space for subsequent restoration of biological communities in the water, making it a material with significant application value and development potential.

[0100] The above description is merely a preferred embodiment of this application. It should be understood that this application is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the concept described herein through the above teachings or the technology or knowledge in related fields. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of this application should be within the protection scope of the appended claims.

Claims

1. A method for preparing remediation materials that simultaneously treat sediment and water, characterized in that, Includes the following steps: Al-MOFs and mixed activated carbon powder were dispersed in an organic solvent, shaken, cooled to room temperature, and then subjected to a first filtration, a first washing, and a first drying to obtain aluminum-based MOF porous activated carbon material. The aluminum-based MOF porous activated carbon material is added to a first calcium lactate solution and stirred for the first time. During the first stirring, a first sodium alginate solution is added. After positive spheroidization, the material is filtered, washed, and dried to obtain aluminum-based MOF porous activated carbon material gel spheres. The aluminum-based MOF porous activated carbon material gel balls and composite bacterial agent were added to water and placed in a shaker for oscillation culture. Then, a third filtration, a third washing, and a third drying were performed to obtain a porous material containing bacteria. The bacterial porous material is added to a second sodium alginate solution and stirred for the second time. During the second stirring process, a second calcium lactate solution is added dropwise. After reverse spheroidization, the material undergoes a fourth filtration, a fourth washing, and a fourth drying to obtain the remediation material that simultaneously treats sediment and water. The Al-MOFs include one of MIL-53 (Al) and CAU-1; And / or, the mixed activated carbon powder includes wood-based activated carbon powder, coal-based activated carbon powder, and fruit shell activated carbon powder; And / or, the compound microbial agent includes photosynthetic bacteria, Bacillus, phosphorus-fixing bacteria, denitrifying bacteria, and nitrate-reducing bacteria.

2. The method according to claim 1, characterized in that, The weight ratio of the Al-MOFs, the mixed activated carbon powder, and the composite microbial agent is 2:(2~4):(1~2). And / or, the weight ratio of the wood-based activated carbon powder, the coal-based activated carbon powder, and the fruit shell activated carbon powder is (10~20):(5~15):(3~7). And / or, the weight ratio of the photosynthetic bacteria, the Bacillus, the phosphorus-fixing bacteria, the denitrifying bacteria and the nitrate-reducing bacteria is (5~9):(8~16):(9~21):(3~7):(2~6).

3. The method according to claim 1, characterized in that, The concentrations of the first sodium alginate solution and the second sodium alginate solution are 1.0~2.0 wt%; And / or, the concentrations of the first calcium lactate solution and the second calcium lactate solution are 2.0~3.0 wt%.

4. The method according to claim 1, characterized in that, The organic solvent includes one of ethanol and dimethylformamide; And / or, the water includes ddH2O.

5. The method according to claim 1, wherein The oscillation method includes ultrasonic oscillation, the oscillation temperature is 160~180℃, and the oscillation time is 24~36 h; And / or, the temperature of the shaking culture is 35~37℃, the shaking culture speed is 160~180 rpm, and the shaking culture time is 48~72 h.

6. The remediation material that simultaneously treats sediment and water body as prepared by the method according to any one of claims 1 to 5.

7. The method described in any one of claims 1 to 5 or the application of the remediation material described in claim 6, including one of the following: water body remediation, sediment remediation, and simultaneous remediation of water body and sediment.

Images