Automatic culture device for noctilucent algae

Abstract

According to the automatic culture device for the noctilucent algae, the sterile seawater, the bait algae solution for ingestion during culture of the noctilucent algae, the noctilucent algae mother solution and the noctilucent algae culture area are arranged in a separated mode, and automatic culture of the noctilucent algae is achieved through timed and quantitative liquid conveying among all the separated areas and stirring control over the liquid. The low-cost automatic culture bioreactor for the noctilucent algae, provided by the invention, can realize efficient, controllable and sterile propagation of the noctilucent algae, and can realize the proliferation of cells of the noctilucent algae by 5.6 times within 14 days, so that the requirements of ornamental and scientific research are met.

Description

Technical Field

[0001] The invention belongs to the technical fields of automated algae cultivation, microalgae biology and marine biology, and in particular relates to an automated cultivation device for Noctiluca scintillans. Background Art

[0002] The "blue tears" landscape is a natural phenomenon of fluorescence in the seawater caused by the massive reproduction or aggregation of organisms such as Noctiluca scintillans in the ocean. From the end of March to the beginning of June every year, the coastal waters of Pingtan-Xiamen in Fujian, China, will attract a large number of tourists to watch and "catch tears" because of the "blue tears". The "blue tears" tourist landscape is bringing positive effects to the local tourism industry. Noctiluca scintillans ) reproduce in large numbers in the near sea, which is the main cause of "blue tears". Although Noctiluca scintillans itself does not produce toxins, when it reproduces in large numbers, the ammonia it secretes can cause the death of marine life, and at the same time consume a large amount of dissolved oxygen in the water, causing water hypoxia. In addition, studies have shown that Noctiluca scintillans will also prey on fish eggs and fry in the water, resulting in a decrease in fish production, which in turn has a serious impact on aquaculture. Given the biological characteristics of Noctiluca scintillans, the natural reproduction of Noctiluca scintillans in marine areas will not only damage the ecological environment, but also have uncertainty in the time and space of occurrence, and the public's appreciation of the phenomenon of the fluorescent sea is limited.

[0003] Noctiluca belongs to the phylum Dinophyta, class Dinophyceae, order Noctilucales, family Noctiluaceae, and genus Noctiluca. It is divided into two types: red Noctiluca and green Noctiluca. All species of Noctiluca recorded along the coast of China are red Noctiluca. Red Noctiluca is a purely heterotrophic algae that needs to obtain energy through predation. In the process of cultivating red Noctiluca, bait algae need to be added to provide energy for Noctiluca. However, the choice of bait algae and the amount of addition will affect the growth of Noctiluca. The seawater temperature and salinity, nutrient level, and seawater disturbance conditions for cultivating Noctiluca also play an important role. At present, there is no mature solution for large-scale cultivation of Noctiluca, and mass cultivation of Noctiluca requires a lot of manpower and time. Summary of the invention

[0004] In order to solve the problems of untimely feeding and large labor costs in large-scale cultivation of Noctiluca, the present invention discloses a low-cost automatic cultivation device for Noctiluca. The device accurately controls the liquid delivery and stirring in each reactor in the device through an automatic control module to simulate the marine growth environment of Noctiluca, and feeds the Noctiluca with prey accurately and on time, so that the Noctiluca in the reactor obtains sufficient bait and sustainable growth. By setting the specific stirring and feeding time, the number of feedings, and the feeding ratio, the efficiency and controllability of Noctiluca in cultivation can be significantly improved, while reducing the risk of algae liquid pollution and Noctiluca cell extinction caused by manual operation, which is suitable for scientific research, teaching demonstration and industrial application of Noctiluca. The low-cost automatic cultivation bioreactor for Noctiluca provided can realize the efficient, controllable and sterile propagation of Noctiluca, and can achieve a 5.6-fold proliferation of Noctiluca cells within 14 days, meeting the needs of viewing and scientific research.

[0005] The technical solution specifically adopted by the present invention to solve the technical problem is: A device for automatically cultivating Noctiluca scintillans: sterile seawater, algae liquid used as bait for cultivation of Noctiluca scintillans, Noctiluca mother liquid and Noctiluca scintillans cultivation area are separated and arranged; automatic cultivation of Noctiluca scintillans is achieved through timed and quantitative liquid transportation between each separated area and stirring control of the liquid.

[0006] Further, including: Four sealed reactors, wherein the first reactor (101) is filled with sterile seawater, the second reactor (102) is filled with bait algae liquid, the third reactor (103) is filled with Noctiluca mother liquid, and the fourth reactor (104) is used to hold the finished product Noctiluca after cultivation and maturity; A liquid transport module, used for transporting liquid between various reactors; A deceleration stirring module, used to stir the liquid inside the reactor; And, an automatic control module connecting the liquid delivery module and the speed reduction stirring module.

[0007] Furthermore, the liquid delivery module comprises a first peristaltic pump (203) connected to the first reactor (101) and the third reactor (103) via a pipeline, a second peristaltic pump (204) connected to the second reactor (102) and the third reactor (103) via a pipeline, and a third peristaltic pump (205) connected to the third reactor (103) and the fourth reactor (104) via a pipeline.

[0008] Furthermore, the liquid delivery module also includes a first Hall flowmeter (201) arranged on the pipeline where the first peristaltic pump (203) is located, and a second Hall flowmeter (202) arranged on the pipeline where the second peristaltic pump (204) is located.

[0009] Furthermore, the deceleration stirring module comprises a first deceleration stirring device (301) and a second deceleration stirring device (302), which are respectively arranged in the second reactor (102) and the third reactor (103), and are used to stir the liquid inside the reactor to simulate the tidal changes of seawater movement, so that the internal liquid is mixed evenly.

[0010] Furthermore, the automatic control module is used to control the start and stop of the peristaltic pump and the reduction stirring motor, and monitor the real-time flow data collected by the Hall flow meter.

[0011] Furthermore, the automatic control module controls the feeding of bait algae liquid in the Noctiluca scintillans and the movement of simulated seawater during the cultivation of the Noctiluca scintillans by controlling the delivery time and delivery volume of each peristaltic pump and the rotation time of the stirring motor of each reduction stirring device to realize the automated cultivation of the Noctiluca scintillans.

[0012] Furthermore, the reactor was placed at a temperature of 20°C and a light intensity of 80 μmol•m -2 •s -1 , in an environment with a light-dark cycle of 14:10h.

[0013] Further, in the initial state, the first reactor (101) is filled with 1L of sterile seawater, the second reactor (102) is filled with 1L of bait algae liquid, and the third reactor (103) is filled with 1.5L of Noctiluca scintillans mother liquid; in the second reactor (102), 100mL of bait algae liquid is transported to the third reactor (103) through the second peristaltic pump (204) and the second Hall flowmeter (202) at the initial start-up and every 3 days thereafter for the Noctiluca to prey on and provide nutrition, and then sterile seawater is added to the first reactor (101) to 2L; in the first reactor (101), 50mL of sterile seawater is transported to the third reactor (103) through the first peristaltic pump (204) and the second Hall flowmeter (202) at the initial start-up and every 3 days thereafter. 3) and the first Hall flowmeter (201) are transported to the third reactor (103); in the third reactor (103), except for the initial start-up, after receiving the liquid transmitted by the first reactor (101) and the second reactor (102), the Noctiluca scintillans liquid in the third reactor (103) is transported to the fourth reactor (104) through the second peristaltic pump (204) to transfer 150 mL of the finished Noctiluca scintillans for subsequent cultivation or transfer; since the start-up of the device, the reduction stirring motors provided in the second reactor (102) and the third reactor (103) are started once every day, and the running time is 3 minutes.

[0014] Furthermore, after the peristaltic pump and the reduction stirring motor finish running each time, 1 mL of the liquid in the second reactor (102) and the third reactor (103) is randomly sampled, and the Noctiluca scintillans and bait algae individuals are observed under a microscope and recorded.

[0015] In the above scheme, the automated cultivation cycle of Noctiluca scintillans is 14 days.

[0016] Compared with the prior art, the beneficial effects of the present invention and its preferred embodiments include at least: 1. Simple and convenient operation, which can effectively reduce the workload of manpower.

[0017] 2. The effect is significant, which further improves the feasibility of cultivating Noctiluca scintillans in the environment.

[0018] 3. Installation suggestions: Liquid delivery and stirring can be set by simply connecting an external load to the circuit. Installation and disassembly are relatively simple.

[0019] 4. Reasonable process and low cost.

[0020] Through this device, the materials in the Noctiluca cultivation tank can be replenished in time, and the Noctiluca can be given sufficient nutrients to meet its reproduction needs. It can not only replenish the nutrients of the Noctiluca in time, but also simulate the movement of the Noctiluca in the outdoor ocean, effectively improve the growth of the Noctiluca in the environment to meet the luminous effect; it does not require a lot of operation by the staff, saving time and manpower, financial and material resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention is further described in detail below with reference to the accompanying drawings and specific embodiments: Figure 1 This is a system block diagram of the automated cultivation device for Noctiluca scintillans according to an embodiment of the present invention.

[0022] Figure 2 This is a physical picture of the automated cultivation device for Noctiluca scintillans according to an embodiment of the present invention.

[0023] Figure 3 This is a diagram of the main control chip of the automatic control module according to an embodiment of the present invention.

[0024] Figure 4 It is a schematic diagram of flow transport of a bait algae reactor and a Noctiluca scintillans reactor according to an embodiment of the present invention.

[0025] Figure 5 This is a diagram of biological indicators related to the Noctiluca scintillans reactor according to an embodiment of the present invention. DETAILED DESCRIPTION

[0026] In order to make the features and advantages of the present invention more clearly understood, the following embodiments are specifically described in detail as follows: It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used in this specification have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0027] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0028] Reference Figure 1 to Figure 2 The embodiment of the present invention provides an automatic cultivation device for Noctiluca scintillans based on STM32, comprising: The reactor is sealed inside and has a volume capacity of 5L, and is used to contain liquids such as seawater and bait algae required for cultivating Noctiluca scintillans; A liquid transport module, connected to the reactor, for transporting liquid between the reactors; A deceleration stirring module is connected to the reactor and is used to stir the liquid inside the reactor; The automatic control device is connected to the external pins of the liquid delivery module, the flow sensor module, and the deceleration stirring module, and is used to control the automatic culture device.

[0029] The device provided in this embodiment mainly controls the feeding of bait algae liquid in Noctiluca scintillans and the movement of simulated seawater during the cultivation of Noctiluca scintillans by controlling the delivery time and delivery volume of different liquid delivery pumps and the rotation time of the stirring motor to realize the automated cultivation of Noctiluca scintillans.

[0030] In this embodiment, different installation tasks are set for the four reactors, wherein the first reactor 101 is filled with 1L of sterile seawater, the second reactor 102 is filled with 1L of algae liquid for Noctiluca prey, the third reactor 103 is filled with 1.5L of Noctiluca mother liquid, and the fourth reactor 104 is used as a tank for the finished product of Noctiluca after cultivation; As a preferred solution of this embodiment, the algae liquid used as bait in the second reactor 102 is a species of Platyhelminthes; the culture liquid is transferred to a glass bottle sterilized by high pressure steam in a sterile operating table, and Platyhelminthes liquid (seawater: mother liquid = 9:1) is injected. Then it is placed in an environment for cultivation, and when the bait algae is in a stable growth state, 1L of the algae liquid is injected into the second reactor 102; As a preferred solution of this embodiment, a Noctiluca sample is taken from a surface seawater sample, and the Noctiluca species is purified by picking single cells from the water sample, and the single cells are transferred to a conical flask in sterile seawater sterilized at 110°C to obtain a Noctiluca seed solution; Platyhelminthes is used as bait and fed three times a week, and the Noctiluca is amplified when the density of the Noctiluca is high; 1.5L of the Noctiluca solution after amplification (with an abundance of 10 Cells / mL) is injected into the third reactor 103.

[0031] In this embodiment, the liquid delivery device includes: a first Hall flowmeter 201 and a second Hall flowmeter 202, a first peristaltic pump 203, a second peristaltic pump 204 and a third peristaltic pump 205; Among them, the Hall flowmeter is used to accurately control the flow rate of each liquid delivery process; the peristaltic pump is used to transport the liquid in each reactor.

[0032] In this embodiment, the Hall flowmeter is a YF-S401 Hall flowmeter, which realizes the acquisition of water flow sensor through digital pulse signals; the core components of the Hall flowmeter are a Hall effect sensor and an impeller placed in the fluid channel. When water or other fluids pass through the sensor, the impeller is pushed by the water flow and rotates. A magnet is installed on the impeller. As the impeller rotates, the magnet approaches or moves away from the Hall effect sensor; when the magnet passes through the Hall flowmeter, it will produce a magnetic field change, causing the Hall flowmeter to output a pulse signal; therefore, the number of times the impeller rotates is proportional to the amount of water flowing through the sensor; the pulse frequency output by the sensor is proportional to the water flow velocity, that is, the faster the flow velocity, the more pulses are generated per unit time; the speed of the water flow is determined by counting the number of pulses generated within a certain period of time.

[0033] The peristaltic pump adopts DP-DIY type pump; DP-DIY type peristaltic pump realizes accurate liquid delivery through roller squeezing hose, which can ensure the safety of liquid delivery process, will not damage the cell structure of Noctiluca and bait algae, and can finely control the flow of liquid; In terms of the specific installation and connection structure, the first reactor 101 is perforated at the top and connected to the first Hall flowmeter 201 and one end of the first peristaltic pump 203 through a PVC transparent hose, and the other end of the first peristaltic pump 203 is perforated and connected to the third reactor 103; the second reactor 102 is perforated at the top and connected to the second Hall flowmeter 202 and one end of the second peristaltic pump 204 through a PVC transparent hose, and the other end of the second peristaltic pump 204 is perforated and connected to the third reactor 103; another hole is perforated at the top of the third reactor 103 and connected to one end of the third peristaltic pump 205 through a PVC transparent hose, and the other end of the third peristaltic pump 205 is perforated and connected to the fourth reactor 104.

[0034] In the device of this embodiment, a first deceleration stirring device 301 and a second deceleration stirring device 302 are also used to stir the liquid inside the reactor to simulate the tidal changes of seawater movement so that the internal liquid is mixed evenly; As a preferred solution of this embodiment, the reduction stirring device is composed of a GA12-N reduction gear motor and a stirring blade; The first deceleration stirring device 301 and the second deceleration stirring device 302 are respectively connected to the second reactor 102 and the third reactor 103 after being perforated.

[0035] In this embodiment, the automatic control device 401 is composed of a single chip, which is used to control the operation of the reaction system; each YF-S401 Hall flow meter, each DP-DIY peristaltic pump, and the power pins of the reduction stirring motor are respectively connected to the corresponding external load pins of the single chip; The automatic control device 401 controls the start and stop of the DP-DIY peristaltic pump and the reduction stirring motor, monitors and controls the real-time flow data collected by the YF-S401 Hall flow meter, displays the real-time flow data and start and stop status on the single-chip microcomputer, and realizes the control of the reaction system.

[0036] Reference Figure 3 , the automatic control device 401 mainly uses STM32F103CBT6 as the main control chip. The operating frequency of STM32F103CBT6 is 72MHz, the power supply voltage is 3~3.6V, and each circuit is connected through different pins. STM32F103CBT6 is based on the ARM Cortex-M3 core and provides high-speed processing performance. Its minimum system configuration uses the internal high-speed clock (HSI) as the main clock source. HSI provides a clock frequency of about 16MHz. At the same time, the BOOT pin is set to a low level to ensure that the program chip is started from the flash memory. The J-Link download mode is used to facilitate program updates and hardware debugging. All power pins are equipped with filter capacitors to ensure the stability and anti-interference ability of the power supply. STM32F103CBT6 is equipped with up to 10 12-bit ADC channels, which can be used independently or in dual mode to increase the sampling rate. The ADC module can measure data from 10 external signal sources and 2 internal signal sources, and perform single, continuous, scan or intermittent mode conversion. During system operation, the interrupt mechanism allows the normal program flow to be interrupted when necessary, and the conversion of the injection channel can take precedence over the conversion of the regular channel, ensuring the real-time and accuracy of data acquisition. In this design, the data collected by the ADC is transmitted through the direct memory access (DMA) mechanism, which effectively improves the data processing speed and meets the needs of real-time control. The STM32 main control module includes the STM32F103CBT6 chip and its peripheral circuits, and a reset circuit 501 electrically connected to the corresponding end of the STM32F103CBT6 chip.

[0037] The NRST pin in the reset circuit is connected to the power supply (3.3V) through a pull-up resistor R2 (10kΩ). When a reset is required, the NRST pin can be pulled low by an external signal to trigger the reset process; The ADC module can achieve synchronous conversion with the system clock and support triggering DMA operation requests after the regular conversion is completed, thereby allowing high-speed data transmission, avoiding direct CPU intervention, and improving overall system efficiency. The input clock frequency of the ADC module can reach up to 14MHz, while the conversion time is only 125 clock cycles, and its maximum sampling frequency can reach 1MHz, which fully meets the needs of capturing fast dynamic signals.

[0038] In this embodiment, the above reaction system was placed in the culture conditions of: temperature 20°C, light intensity 80 μmol•m -2 •s -1 , in an environment with a light-dark cycle of 14:10 h.

[0039] Reference Figure 4 , in the second reactor 102, 100mL of bait algae liquid is transported to the third reactor 103 through the second peristaltic pump 204 and the second Hall flowmeter 202 at the initial start-up and every 3 days thereafter for the Noctiluca to prey on and provide nutrition, and then the sterile seawater is added to the first reactor 101 to 2L; in the first reactor 101, 50mL of sterile seawater is also transported to the third reactor 103 through the first peristaltic pump 203 and the first Hall flowmeter 201 at the initial start-up and every 3 days thereafter; in the third reactor 103, except for the initial start-up, after receiving the liquid transmitted by the first reactor 101 and the second reactor 102, the Noctiluca liquid in the third reactor 103 is transported to the fourth reactor 104 through the second peristaltic pump 204 to transfer 150mL of finished Noctiluca for subsequent cultivation or transfer; since the start-up of the device, the reduction stirring motors in the second reactor 102 and the third reactor 103 are started once every 1 day, and the running time is 3 minutes; As a preferred solution of this embodiment, 1 mL of liquid in the second reactor 102 and the third reactor 103 is randomly sampled after each operation of the peristaltic pump and the reduction stirring motor of the device is completed, and the Noctiluca scintillans and bait algae individuals are observed under a microscope, recorded, and their data are processed.

[0040] Reference Figure 5 The invention is used to realize the automatic cultivation of Noctiluca, and a cycle (14 days) of cultivation is carried out; in the third reactor 103, while the Noctiluca is digesting the bait algae, the device can ensure a high concentration of bait algae in the third reactor 103, ensuring that it can continuously obtain continuous nutrition for reproduction; in a cycle of cultivation time, the Noctiluca can achieve continuous growth, and the Noctiluca cells in the third reactor 103 grow fastest in the later stage, and the number of Noctiluca cells has achieved a high-speed reproduction from the original 10 / mL to the final 56 / mL. The Noctiluca cells proliferate 5.6 times, which fully meets the needs of viewing and scientific research.

[0041] In addition, for the device selection of the embodiment of the present invention, the reduction stirring motor adopts the GA12-N reduction gear motor, which is a motor-driven closed transmission gear reduction device, a reduction transmission mechanism that integrates and assembles the motor and the gear box, and is used to reduce the speed and increase the torque to meet the needs of mechanical equipment. The gear motor product has the characteristics of "small size, large torque, and low noise"; the peristaltic pump adopts the DP-DIY pump, which is installed on the L-shaped base, has a low flow rate and a long motor life; the reduction motor of this embodiment is 100RPM (100 revolutions per minute) when 12V is unloaded, and the output shaft length is 100MM, which ensures the simulation of seawater movement in the growth environment of Noctiluca; the flow rate of the peristaltic pump is 2~17mL / min when 12V is unloaded, which meets the liquid delivery flow rate; The main function of the reduction stirring motor is to simulate the natural movement of seawater during the algae cultivation process. The automatic start time of the load reduction motor can be accurately set by programming the real-time clock (RTC) function of the main control chip. By configuring the RTC interrupt service program, the system is designed to automatically start the reduction motor once every 24 hours, and each start lasts for 3 minutes to achieve regular stirring of the algae liquid.

[0042] At a predetermined time point, the RTC triggers an interrupt, and the main control chip executes the interrupt service program, which is responsible for changing the state of the GPIO pin connected to the gate of the MOSFET transistor. When the GPIO outputs a high level, the MOSFET is turned on, the motor power is energized, and the motor starts to rotate, thereby achieving liquid stirring.

[0043] On the contrary, when the GPIO outputs a low level, the MOSFET is turned off, the motor power is cut off, and the motor stops rotating. In this way, the stirring of the motor connected to the bait algae tank and the luminous algae tank is automatically controlled, ensuring the uniform mixing of the liquid in the algae tank.

[0044] In this embodiment, the automatic start of the peristaltic pump is accurately set by programming the real-time clock function of the main control chip. The system is designed to trigger the interrupt service program for the peristaltic pump connected to each cylinder at a set time point to achieve automatic liquid delivery; Two groups of Hall flow meters are used for flow calculation in the liquid transportation process.

[0045] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0046] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

[0047] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of an automated cultivation device for Noctiluca scintillans under the inspiration of the present invention. All equivalent changes and modifications made within the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. An automated cultivation device for Noctiluca scintillans, characterized in that: The sterile seawater, algae liquid used as bait for the cultivation of Noctiluca, Noctiluca mother liquid and Noctiluca cultivation area are separated and set, and the automated cultivation of Noctiluca is achieved through the timed and quantitative liquid transportation between the separated areas and the stirring control of the liquid.

2. The automatic cultivation device for Noctiluca scintillans according to claim 1, characterized in that: include: Four sealed reactors, wherein the first reactor (101) is filled with sterile seawater, the second reactor (102) is filled with bait algae liquid, the third reactor (103) is filled with Noctiluca mother liquid, and the fourth reactor (104) is used to hold the finished product Noctiluca after cultivation and maturity; A liquid transport module, used for transporting liquid between various reactors; A deceleration stirring module, used to stir the liquid inside the reactor; And, an automatic control module connecting the liquid delivery module and the speed reduction stirring module.

3. The automatic cultivation device for Noctiluca scintillans according to claim 2, characterized in that: The liquid delivery module comprises a first peristaltic pump (203) connected to the first reactor (101) and the third reactor (103) via a pipeline, a second peristaltic pump (204) connected to the second reactor (102) and the third reactor (103) via a pipeline, and a third peristaltic pump (205) connected to the third reactor (103) and the fourth reactor (104) via a pipeline.

4. The automatic cultivation device for Noctiluca scintillans according to claim 2, characterized in that: The liquid delivery module further comprises a first Hall flow meter (201) arranged on the pipeline where the first peristaltic pump (203) is located, and a second Hall flow meter (202) arranged on the pipeline where the second peristaltic pump (204) is located.

5. The automatic cultivation device for Noctiluca scintillans according to claim 3, characterized in that: The deceleration stirring module comprises a first deceleration stirring device (301) and a second deceleration stirring device (302), which are respectively arranged in the second reactor (102) and the third reactor (103) and are used to stir the liquid inside the reactor to simulate the tidal changes of seawater movement so that the internal liquid is mixed evenly.

6. The automatic cultivation device for Noctiluca scintillans according to claim 5, characterized in that: The automatic control module is used to control the start and stop of the peristaltic pump and the reduction stirring motor, and monitor the real-time flow data collected by the Hall flow meter.

7. The automatic cultivation device for Noctiluca scintillans according to claim 5, characterized in that: The automatic control module controls the feeding of bait algae liquid in the Noctiluca scintillans and the movement of simulated seawater during the cultivation of the Noctiluca scintillans by controlling the delivery time and delivery volume of each peristaltic pump and the rotation time of the stirring motor of each reduction stirring device to realize the automatic cultivation of the Noctiluca scintillans.

8. The automatic cultivation device for Noctiluca scintillans according to claim 7, characterized in that: The reactor was placed at a temperature of 20°C and a light intensity of 80 μmol•m -2 •s -1 , in an environment with a light-dark cycle of 14:10 h.

9. The automatic cultivation device for Noctiluca scintillans according to claim 8, characterized in that: In an initial state, the first reactor (101) is filled with 1L of sterile seawater, the second reactor (102) is filled with 1L of bait algae liquid, and the third reactor (103) is filled with 1.5L of Noctiluca scintillans mother liquid; in the second reactor (102), 100mL of bait algae liquid is transported to the third reactor (103) through the second peristaltic pump (204) and the second Hall flowmeter (202) at the initial start-up and every 3 days thereafter for the Noctiluca to prey on and provide nutrition, and then sterile seawater is added to the first reactor (101) to 2L; in the first reactor (101), 50mL of sterile seawater is transported to the third reactor (103) through the first peristaltic pump (204) and the second Hall flowmeter (202) at the initial start-up and every 3 days thereafter. 3) and the first Hall flowmeter (201) are transported to the third reactor (103); in the third reactor (103), except for the initial start-up, after receiving the liquid transmitted by the first reactor (101) and the second reactor (102), the Noctiluca scintillans liquid in the third reactor (103) is transported to the fourth reactor (104) through the second peristaltic pump (204) to transfer 150 mL of the finished Noctiluca scintillans for subsequent cultivation or transfer; since the start-up of the device, the reduction stirring motors provided in the second reactor (102) and the third reactor (103) are started once every day, and the running time is 3 minutes.

10. The automatic cultivation device for Noctiluca scintillans according to claim 9, characterized in that: After the peristaltic pump and the reduction stirring motor finish running each time, 1 mL of the liquid in the second reactor (102) and the third reactor (103) is randomly sampled, and the Noctiluca scintillans and bait algae individuals are observed under a microscope and recorded.

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