Diester oil microbial fermentation method and system based on fermentation process optimization

Abstract

The invention relates to the technical field of microbial fermentation regulation and control, in particular to a diester oil microbial fermentation method and system based on fermentation process optimization, and the method comprises the following steps: obtaining fermentation liquor of modified saccharomycetes, and filtering to obtain a diester oil mixture; identifying the total diester oil concentration and the biological waste concentration in the diester oil mixture; calculating the fermentation time according to the backing material injection speed and the fermentation volume; according to the total diester oil concentration and the obtained microorganism concentration, calculating the yield of the diester oil per unit microorganism; according to the microorganism concentration, the fermentation environment comprehensive coefficient, the biological waste concentration, the base material concentration and the unit microorganism diester oil yield, the base material consumption rate and the diester oil generation rate are obtained; and comparing the diester oil generation rate with the backing material consumption rate to obtain a fermentation complete limitation formula, and solving the optimal fermentation environment, the backing material concentration and the backing material injection speed corresponding to the maximum value of the diester oil generation rate in the fermentation complete limitation formula to realize fermentation control. According to the invention, the overall efficiency of diester oil fermentation production can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation regulation and control, and in particular to a diester oil microbial fermentation method and system based on fermentation process optimization. Background Art

[0002] Diester oil is an important class of bio-based compounds, widely used in biofuels, lubricants, plastic additives and food industries. Compared with traditional petroleum-based products, diester oil has the advantages of being renewable, environmentally friendly and biodegradable, and therefore has attracted more and more attention.

[0003] However, current microbial fermentation processes often fail to meet expectations in terms of yield and production rate. In most cases, the growth rate of microorganisms and the rate of product generation do not match, resulting in longer fermentation times and affecting overall production efficiency. Summary of the invention

[0004] The present invention provides a diester oil microbial fermentation method based on fermentation process optimization, the main purpose of which is to optimize the fermentation process of the diester oil based on sustainability and efficiency to improve the overall production efficiency.

[0005] To achieve the above-mentioned purpose, the present invention provides a diester oil microbial fermentation method based on fermentation process optimization, comprising: obtaining modified yeast, and performing a fermentation operation on the modified yeast using a pre-constructed fermentation chamber to obtain a fermentation liquid; injecting a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and filtering the fermentation liquid of the unit time injection volume in the fermentation chamber using a pre-constructed diaphragm to obtain a diester oil mixture, and identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can pass through the biological product of the modified yeast, but cannot pass through the modified yeast; the diester oil mixture is esterified to obtain an artificially synthesized diester oil, the concentration of the artificially synthesized diester oil is calculated based on the artificially synthesized diester oil and the injection volume per unit time, and the concentration of the diester oil is summed up using the concentration of the artificially synthesized diester oil to obtain the total diester oil concentration; the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation bin are obtained, and the fermentation time of the fermentation liquid is calculated based on the injection speed of the base material and the fermentation volume; the fermentation liquid The cells in the fermentation tank are monitored to obtain the microbial concentration, and the unit microbial diester oil production is calculated according to the total diester oil concentration and the microbial concentration; the base material consumption rate is obtained according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration; the base material concentration and the biological waste concentration are used to construct the Monod equation of microbial growth rate, and the diester oil generation rate is obtained according to the microbial concentration, the Monod equation of microbial growth rate and the unit microbial diester oil production; according to the preset complete fermentation strategy, the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time to obtain the fermentation complete restriction formula; according to the fermentation complete restriction formula, the fermentation tank is subjected to random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, the base material concentration and the base material injection speed to obtain the diester oil generation rate change curve, and the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed when the diester oil generation rate is the maximum value in the diester oil generation rate change curve are obtained; according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed, the fermentation tank is continuously injected to obtain a stable diester oil output.

[0006] Optionally, the obtaining of the modified yeast includes: obtaining an initial yeast, enhancing the metabolic pathway based on synthetic esters of the initial yeast, and obtaining a primary modified yeast; monitoring the ester synthesis activity of the primary modified yeast, screening the primary modified yeast with the highest ester synthesis activity, and obtaining a high-yield yeast; breeding the high-yield yeast for a long term to obtain a plurality of breeding strains, and retaining a breeding strain with high ester synthesis stability among the plurality of breeding strains as the modified yeast.

[0007] Optionally, the esterification operation on the diester oil mixture to obtain the synthetic diester oil includes: filtering the diester oil in the diester oil mixture to obtain a bio-product mixture; adding a pre-constructed catalyst to the bio-product mixture, and performing an esterification operation according to a preset esterification temperature to obtain an esterification solution; optically monitoring the esterification solution to obtain an infrared spectrum; when the absorption characteristic peak of the ester in the infrared spectrum no longer changes, filtering the esterification solution to obtain the synthetic diester oil.

[0008] Optionally, the fermentation time is expressed as: , where represents the fermentation time, represents the fermentation volume, Indicates the base material injection speed.

[0009] Optionally, obtaining the base material consumption rate according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration includes: obtaining the growth inhibition constant of the biological waste concentration for the modified yeast, and performing weighted calculation on the biological waste concentration and the growth inhibition constant to obtain the growth inhibition coefficient; performing weighted calculation on the fermentation environment comprehensive coefficient and the base material concentration to obtain the growth promotion coefficient; calculating the difference between the growth promotion coefficient and the growth inhibition coefficient to obtain the growth comprehensive coefficient; performing weighted calculation on the microbial concentration according to the growth comprehensive coefficient to obtain the base material consumption rate, and the base material consumption rate is expressed as: , where represents the base material consumption rate, Indicates the concentration of the base material, represents the comprehensive coefficient of the fermentation environment, including temperature, oxygen concentration and pH value, represents the concentration of the microorganism, represents the biowaste concentration, It represents the growth inhibition constant of the modified yeast by the concentration of the biological waste.

[0010] Optionally, the use of the substrate concentration and the biological waste concentration to construct a Monod equation for microbial growth rate includes: obtaining the maximum growth rate of the modified yeast when the substrate concentration is sufficient, and obtaining the substrate concentration corresponding to the half-value speed of the maximum growth rate to obtain a promoting half-saturation constant of the modified yeast; obtaining an inhibiting half-saturation constant of the biological waste concentration for the modified yeast, performing weighted calculation on the inhibiting half-saturation constant and the biological waste concentration to obtain a growth inhibition influence value, and summing the promoting half-saturation constant and the growth inhibition influence value to obtain a modified half-saturation constant; according to a pre-constructed Monod algorithm, using the substrate concentration, the maximum growth rate and the modified half-saturation constant, calculating the growth rate of the modified yeast to obtain a Monod equation for microbial growth rate, the Monod equation for microbial growth rate is expressed as: , where represents the growth rate of the modified yeast, It indicates the maximum growth rate of the modified yeast when the base material concentration is sufficient. represents the half-saturation constant of the base material concentration promoting the modified yeast, The inhibition half-saturation constant representing the effect of the biological waste concentration on the growth of the modified yeast.

[0011] Optionally, obtaining the diester oil generation rate according to the microbial concentration, the Monod equation of microbial growth rate and the unit microbial diester oil production includes: performing weighted calculation on the microbial concentration and the Monod equation of microbial growth rate to obtain the microbial concentration change rate; performing weighted calculation on the microbial concentration change rate according to the unit microbial diester oil production to obtain the diester oil generation rate, wherein the diester oil generation rate is expressed as: , where represents the diester oil production rate, It represents the unit microbial diester oil production.

[0012] Optionally, the fermentation complete limitation formula is expressed as: Optionally, the method of obtaining the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil production rate in the diester oil production rate change curve is at the maximum value includes: spatially mapping the diester oil production rate change curve in a three-dimensional space constructed according to the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a three-dimensional diester oil production rate matrix; identifying the cluster center points in the three-dimensional diester oil production rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining the values ​​of the cluster center points in each dimension of the three-dimensional space to obtain the optimal fermentation environment, optimal base material concentration and optimal base material injection speed.

[0013] To achieve the above-mentioned object, the present invention also provides a diester oil microbial fermentation system based on fermentation process optimization, comprising: a fermentation module, used to obtain modified yeast, and use a pre-constructed fermentation chamber to perform a fermentation operation on the modified yeast to obtain a fermentation liquid; a diester oil concentration detection module, used to inject a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and use a pre-constructed diaphragm to filter the fermentation liquid of the unit time injection volume in the fermentation chamber to obtain a diester oil mixture, and identify the diester oil concentration and biological waste in the diester oil mixture. The diaphragm can pass the biological product of the modified yeast, but cannot pass the modified yeast, and the diester oil mixture is esterified to obtain an artificially synthesized diester oil, and the concentration of the artificially synthesized diester oil is calculated according to the artificially synthesized diester oil and the injection volume per unit time, and the diester oil concentration is summed up using the artificially synthesized diester oil concentration to obtain the total diester oil concentration; a substrate consumption rate calculation module is used to obtain the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation bin, calculate the fermentation time of the fermentation liquid according to the substrate injection speed and the fermentation volume, and calculate the total diester oil concentration. The cells in the fermentation broth are monitored to obtain the microbial concentration, and the unit microbial diester oil production is calculated according to the total diester oil concentration and the microbial concentration, and the base material consumption rate is obtained according to the microbial concentration, the comprehensive coefficient of the fermentation environment, the biological waste concentration and the base material concentration; the diester oil generation rate calculation module is used to construct the microbial growth rate Monod equation using the base material concentration and the biological waste concentration, and obtain the diester oil generation rate according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production; the fermentation condition adjustment module is used to adjust the said base material concentration and the biological waste concentration according to the preset complete fermentation strategy. The ratio of the diester oil production rate to the base material consumption rate is equivalent to the fermentation time, and the fermentation complete restriction formula is obtained. According to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a diester oil production rate change curve, and the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil production rate is the maximum in the diester oil production rate change curve are obtained. According to the optimal fermentation environment, optimal base material concentration and optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

[0014] In order to solve the above problems, the present invention also provides an electronic device, which includes: a memory storing at least one instruction; and a processor executing the instructions stored in the memory to implement the above-mentioned diester oil microbial fermentation method based on fermentation process optimization.

[0015] In order to solve the above problems, the present invention also provides a computer-readable storage medium, in which at least one instruction is stored, and the at least one instruction is executed by a processor in an electronic device to implement the above-mentioned diester oil microbial fermentation method based on fermentation process optimization.

[0016] The present invention is to solve the problem described in the background technology. The present invention first designs a fermentation bin with balanced input and output to ferment the modified yeast to obtain a fermentation liquid. By measuring the total diester oil concentration and microbial concentration of the fermentation liquid, the unit microbial diester oil production can be calculated, thereby estimating the diester oil generation rate; measuring the biological waste concentration of the fermentation liquid, the microbial growth rate can be estimated, thereby estimating the base material consumption rate; due to the complete fermentation strategy of the present invention, the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time, thereby further limiting the diester oil generation rate, and finally obtaining the relationship between the diester oil generation rate and the comprehensive coefficient of the fermentation environment, the base material concentration and the base material injection speed, thereby experimentally obtaining the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed, and using them to guide the output of diester oil. Therefore, the present invention optimizes the fermentation process of diester oil based on sustainability and efficiency to improve overall production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of a diester oil microbial fermentation method based on fermentation process optimization provided in one embodiment of the present invention; Figure 2 A functional module diagram of a diester oil microbial fermentation system based on fermentation process optimization provided by one embodiment of the present invention; Figure 3 A schematic diagram of the structure of an electronic device for implementing the diester oil microbial fermentation method based on fermentation process optimization provided by one embodiment of the present invention.

[0018] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0019] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0020] The embodiment of the present application provides a diester oil microbial fermentation method based on fermentation process optimization. The execution subject of the diester oil microbial fermentation method based on fermentation process optimization includes but is not limited to at least one of the electronic devices such as a server and a terminal that can be configured to execute the method provided in the embodiment of the present application. In other words, the diester oil microbial fermentation method based on fermentation process optimization can be executed by software or hardware installed in a terminal device or a server device, and the software can be a blockchain platform. The server includes but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, etc.

[0021] Reference Figure 1 FIG. 1 is a flow chart of a diester oil microbial fermentation method based on fermentation process optimization provided by an embodiment of the present invention. In this embodiment, the diester oil microbial fermentation method based on fermentation process optimization includes: S1, obtaining modified yeast, and performing fermentation operation on the modified yeast in a pre-constructed fermentation chamber to obtain a fermentation liquid.

[0022] In the embodiment of the present invention, the modified yeast refers to a microorganism whose metabolic pathway for synthesizing esters is enhanced by genetic engineering. In addition, microbial samples (such as soil, water, and plants) can also be extracted from the natural environment to screen out microorganisms that have the ability to produce fatty acids and alcohols, thereby performing modification.

[0023] Furthermore, the fermentation chamber includes a chamber, an inlet, an outlet, a diaphragm and an environmental control port. The chamber is the actual place where the fermentation process takes place; the inlet is used to put in the base material; the diaphragm is used to retain the modified yeast in the chamber, while allowing the diester oil mixture formed by biological products such as esters, acids, diester oils and biological waste to pass through; the outlet is used to discharge the diester oil mixture, and a filtering function can also be added to directly extract the diester oil; the environmental control port is used to control the environment in the chamber, such as temperature, pH value and oxygen concentration.

[0024] In detail, in an embodiment of the present invention, the method of obtaining the modified yeast comprises: obtaining an initial yeast, enhancing the metabolic pathway based on synthetic esters of the initial yeast, and obtaining a primary modified yeast; monitoring the ester synthesis activity of the primary modified yeast, screening the primary modified yeast with the highest ester synthesis activity, and obtaining a high-yield yeast; breeding the high-yield yeast for a long term to obtain a plurality of breeding strains, and retaining the breeding strain with high ester synthesis stability among the plurality of breeding strains as the modified yeast.

[0025] The initial yeast is the common oil yeast Yarrowia lipolytica in the laboratory.

[0026] The present invention first observes the metabolic pathway of synthetic esters, finds fatty acid synthase (FAS) and enzymes related to fatty acid synthesis in yeast, then selects important genes (such as FAS, ACPs related genes) for cloning, constructs expression vectors, and obtains primary modified yeast.

[0027] Then, the present invention detects the ester synthesis activity of the microorganism by measuring the concentration of ester compounds in the fermentation broth, and screens out high-yield yeast. Finally, through long-term cultivation and selection, the metabolic capacity of the microorganism is enhanced, so that it can synthesize ester compounds more stably and effectively under specific conditions, thereby obtaining modified yeast.

[0028] In the embodiment of the present invention, the modified yeast is used for fermentation to obtain a fermentation liquid, wherein nutrients, biological waste and biological products are present in the fermentation liquid.

[0029] S2. According to a preset base material injection speed, a base material of a preset base material concentration is injected into the fermentation bin to obtain a unit time injection volume of the base material, and a pre-constructed diaphragm is used to filter the fermentation liquid of the unit time injection volume in the fermentation bin to obtain a diester oil mixture, and the diester oil concentration and the biological waste concentration in the diester oil mixture are identified, wherein the diaphragm can pass the biological products of the modified yeast but cannot pass the modified yeast.

[0030] In the embodiment of the present invention, the base material includes and contains at least water, nutrients, and alcohol and acid required for esterification reaction, and the nutrients include sugars, amino acids, minerals and vitamins, etc. The base material concentration refers to the volume ratio of substances other than water in the base material.

[0031] In the embodiment of the present invention, the volume of the fermentation bin is constant. To achieve continuous production, the volume of the feed volume and the discharge volume must be the same. In order to ensure the completeness of the fermentation process, the base material must be completely consumed from the feed inlet to the discharge outlet, thereby improving the fermentation efficiency and reducing the waste of the base material.

[0032] In the embodiment of the present invention, the preset unknown number Indicates the base material injection speed.

[0033] Specifically, in the embodiment of the present invention, according to the injection speed of the bottom material , per unit time In the fermentation tank, the volume per unit time is injected Then we get The volume of fermentation broth is filtered through a diaphragm to obtain a diester oil mixture.

[0034] Furthermore, in the embodiment of the present invention, it is also necessary to identify the diester oil concentration and the biological waste concentration in the diester oil mixture. The diester oil concentration can be used to preliminarily evaluate the production capacity of the modified yeast, while the biological waste concentration can be used to determine whether the living environment of the microorganisms is healthy. The diester oil concentration and the biological waste concentration are the volume ratios of the diester oil and the biological waste in the volume injected per unit time.

[0035] S3. Performing an esterification operation on the diester oil mixture to obtain synthetic diester oil, calculating the concentration of the synthetic diester oil according to the synthetic diester oil and the injection volume per unit time, and summing the diester oil concentrations using the synthetic diester oil concentration to obtain a total diester oil concentration.

[0036] In the embodiment of the present invention, the concentration of the liquid substances in each base material, biological product or biological waste in the fermentation broth, such as the artificial synthetic diester oil and subsequent various solutions, is calculated based on the injection volume per unit time, and the volume of each substance is identified.

[0037] In detail, in an embodiment of the present invention, the esterification operation on the diester oil mixture to obtain an artificially synthesized diester oil includes: filtering the diester oil in the diester oil mixture to obtain a bio-product mixture; adding a pre-constructed catalyst to the bio-product mixture, and performing an esterification operation according to a preset esterification temperature to obtain an esterification solution; optically monitoring the esterification solution to obtain an infrared spectrum; when the absorption characteristic peak of the ester in the infrared spectrum no longer changes, filtering the esterification solution to obtain an artificially synthesized diester oil.

[0038] Specifically, the present invention first filters the diester oil synthesized by the modified yeast itself and the diester oil produced by the ester biological product according to the reaction between the acid and alcohol in the base material by means of fractionation.

[0039] However, the esterification reaction has certain reversibility and certain catalytic conditions. Therefore, the diester oil mixture still has a large amount of biological products, which can be processed by subsequent catalysis to obtain artificially synthesized diester oil.

[0040] The present invention uses phosphoric acid as a catalyst and sets the esterification temperature to 140° C. to catalyze the biological product mixture to obtain an esterification solution. Then, the absorption characteristic peaks of esters in the esterification solution are monitored by a spectrometer to obtain an infrared spectrum. When the absorption characteristic peaks of esters in the infrared spectrum no longer change, the esterification solution is filtered to obtain an artificially synthesized diester oil.

[0041] Since the synthetic diester oil and the biosynthesized diester oil are taken from the same solution, the concentration of the synthetic diester oil can be directly measured and summed with the concentration of the diester oil to obtain the total diester oil concentration.

[0042] S4. Obtain the fermentation volume and comprehensive coefficient of the fermentation environment of the fermentation bin, and calculate the fermentation time of the fermentation liquid according to the injection speed of the base material and the fermentation volume.

[0043] In the embodiment of the present invention, the fermentation volume is a fixed constant , and the fermentation environment comprehensive coefficient is a variable constant. The fermentation environment comprehensive coefficient configured in the present invention is , containing quantitative information on temperature, oxygen concentration and pH value.

[0044] According to the ratio of the injection speed of the base material and the fermentation volume, the present invention can obtain the proportion of the injection volume per unit time in the fermentation bin, for example, one tenth, which means that ten unit time injections are required to completely replace the fermentation liquid in the fermentation bin. In order to ensure that each injection is completely fermented and not wasted, the fermentation time of the base material of the injection volume per unit time needs to be completely fermented within ten unit time. Too early will lead to reduced fermentation efficiency, too long will lead to incomplete fermentation of the base material, thereby causing waste of the base material.

[0045] Therefore, in the embodiment of the present invention, the fermentation time is defined as the ratio of the fermentation volume to the injection speed of the base material.

[0046] In detail, in the embodiments of the present invention, the fermentation time is expressed as: , where represents the fermentation time, represents the fermentation volume, Indicates the base material injection speed.

[0047] S5. Monitoring the cells in the fermentation broth to obtain the microbial concentration, and calculating the unit microbial diester oil yield based on the total diester oil concentration and the microbial concentration.

[0048] In the embodiment of the present invention, an optical density sensor is used to monitor the turbidity of the microbial culture solution in real time to estimate the microbial concentration, wherein the microbial concentration refers to the total number of modified yeasts in the injected volume per unit time.

[0049] Furthermore, in the embodiments of the present invention, the volume of total diester oil concentration and microorganism concentration is the same, which is the injection volume per unit time. Therefore, a direct comparison can be made to obtain the unit microbial diester oil yield.

[0050] S6. Obtaining a base material consumption rate according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration.

[0051] It should be known that the base material consumption rate is proportional to the base material concentration, proportional to the microorganism concentration, and inversely proportional to the biological waste concentration.

[0052] In detail, in an embodiment of the present invention, the base material consumption rate is obtained according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration, including: obtaining the growth inhibition constant of the biological waste concentration for the modified yeast, and weightedly calculating the biological waste concentration and the growth inhibition constant to obtain the growth inhibition coefficient; weighted calculating the fermentation environment comprehensive coefficient and the base material concentration to obtain the growth promotion coefficient; calculating the difference between the growth promotion coefficient and the growth inhibition coefficient to obtain the growth comprehensive coefficient; weighted calculating the microbial concentration according to the growth comprehensive coefficient to obtain the base material consumption rate, and the base material consumption rate is expressed as: , where represents the base material consumption rate, Indicates the concentration of the base material, represents the comprehensive coefficient of the fermentation environment, including temperature, oxygen concentration and pH value, represents the concentration of the microorganism, represents the biowaste concentration, It represents the growth inhibition constant of the modified yeast by the concentration of the biological waste.

[0053] The growth inhibition coefficient indicates that the growth rate of the modified yeast is reduced and the cell activity is reduced, while the growth promotion coefficient indicates that the base material promotes the growth of the modified yeast.

[0054] The present invention makes a difference between the growth inhibition coefficient and the growth promotion coefficient to obtain a comprehensive growth coefficient, thereby calculating the base material consumption rate of the base material by the microorganism according to the microorganism concentration. The comprehensive growth coefficient is used to represent the comprehensive impact on the modified yeast.

[0055] S7. Using the base material concentration and the biological waste concentration, construct a Monod equation for microbial growth rate, and obtain the diester oil production rate based on the microbial concentration, the Monod equation for microbial growth rate and the unit microbial diester oil production.

[0056] In the embodiment of the present invention, the Monod equation is a model for describing the growth rate of microorganisms under changes in nutrient concentration.

[0057] In detail, in an embodiment of the present invention, the use of the base material concentration and the biological waste concentration to construct a Monod equation for microbial growth rate includes: obtaining the maximum growth rate of the modified yeast when the base material concentration is sufficient, and obtaining the base material concentration corresponding to the half-value speed of the maximum growth rate to obtain the promotion half-saturation constant of the modified yeast; obtaining the inhibition half-saturation constant of the biological waste concentration for the modified yeast, weighted calculation of the inhibition half-saturation constant and the biological waste concentration to obtain a growth inhibition influence value, and summing the promotion half-saturation constant and the growth inhibition influence value to obtain a modified half-saturation constant; according to a pre-constructed Monod algorithm, the base material concentration, the maximum growth rate and the modified half-saturation constant are used to calculate the growth rate of the modified yeast to obtain the Monod equation for microbial growth rate, and the Monod equation for microbial growth rate is expressed as: , where represents the growth rate of the modified yeast, It indicates the maximum growth rate of the modified yeast when the base material concentration is sufficient. represents the half-saturation constant of the base material concentration promoting the modified yeast, The inhibition half-saturation constant representing the effect of the biological waste concentration on the growth of the modified yeast.

[0058] In the embodiment of the present invention, the base material concentration is continuously increased, the real-time growth rate of the yeast is recorded, a growth rate curve is obtained, and then the peak growth rate is obtained to obtain the maximum growth rate G, and then the base material concentration corresponding to the G / 2 speed is obtained to obtain the promotion half-saturation constant.

[0059] Similarly, the inhibition half-saturation constant of the biological waste concentration on the modified yeast is obtained. Then, according to the real-time biological waste concentration, the growth inhibition impact value of the biological waste concentration on the modified yeast is calculated.

[0060] In the embodiment of the present invention, in the Monod equation On this basis, the effect of biological waste concentration on the growth of the modified yeast was added to obtain a modified half-saturation constant, which was then brought into various parameters to obtain the Monod equation for microbial growth rate.

[0061] In detail, in the embodiment of the present invention, the diester oil generation rate is obtained according to the microbial concentration, the Monod equation of microbial growth rate and the unit microbial diester oil production, including: performing weighted calculation on the microbial concentration and the Monod equation of microbial growth rate to obtain the microbial concentration change rate; performing weighted calculation on the microbial concentration change rate according to the unit microbial diester oil production to obtain the diester oil generation rate, and the diester oil generation rate is expressed as: , where represents the diester oil production rate, It represents the unit microbial diester oil production.

[0062] Specifically, in the embodiment of the present invention, according to The change rate of microbial concentration is obtained, and then the unit microbial diester oil production is calculated. , thereby obtaining the diester oil production rate.

[0063] S8. According to a preset complete fermentation strategy, the ratio of the diester oil production rate to the base material consumption rate is equated to the fermentation time to obtain a complete fermentation restriction formula.

[0064] In the embodiment of the present invention, the complete fermentation strategy means that the base material injected once can be completely fermented before being discharged.

[0065] The present invention equates the ratio of the injection volume per unit time to the base material consumption rate to the fermentation time. However, the injection volume per unit time is the same as the volume of the discharged fermentation liquid, and diester oil is the target component in the fermentation liquid. Therefore, the present invention utilizes the diester oil generation rate to replace the injection volume per unit time.

[0066] In detail, in the embodiment of the present invention, the fermentation complete restriction formula is expressed as: , through calculation, we get: , when the fermentation volume Fixed, while the concentration of biowaste Base material injection speed , base material concentration and fermentation environment comprehensive coefficient and can be directly measured, it can be seen that the diester oil production rate changes with the base material injection speed. , base material concentration and fermentation environment comprehensive coefficient changes with the changes of.

[0067] S9. According to the complete fermentation restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a diester oil production rate change curve, and obtain the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil production rate is the maximum in the diester oil production rate change curve.

[0068] In the embodiment of the present invention, a control experiment can be designed to change one or more conditions of the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to record the output of diester oil, thereby obtaining a diester oil generation rate change curve.

[0069] In detail, in an embodiment of the present invention, the method of obtaining the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil production rate in the diester oil production rate change curve is the maximum value includes: spatially mapping the diester oil production rate change curve in a three-dimensional space constructed according to the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a three-dimensional diester oil production rate matrix; identifying the cluster center points in the three-dimensional diester oil production rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining the values ​​of the cluster center points in each dimension of the three-dimensional space to obtain the optimal fermentation environment, optimal base material concentration and optimal base material injection speed.

[0070] In the embodiment of the present invention, the comprehensive coefficient of the fermentation environment, the base material concentration and the base material injection speed each represent the three dimensions of x, y, and z, thereby constructing a three-dimensional space, and mapping the diester oil generation rate change curve into the three-dimensional space. Since each test result of the present invention is a test performed within the variation range of the three-dimensional data, the diester oil generation rate change curve will eventually be mapped into a spherical data structure. The present invention selects a K-means clustering algorithm that has effective clustering for spherical data structures, presets K initial center points, and iteratively updates the center points until convergence to obtain cluster center points. Then, the values ​​of the three dimensions corresponding to the cluster center points are obtained to obtain the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed.

[0071] S10, continuously injecting material into the fermentation bin according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed to obtain a stable diester oil output.

[0072] The value at the cluster center has a higher diester oil generation rate, and the surrounding numerical points also have a high diester oil generation rate. The optimal fermentation environment, optimal base material concentration and optimal base material injection speed corresponding to the cluster center have good stability.

[0073] Therefore, according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

[0074] The present invention is to solve the problem described in the background technology. The present invention first designs a fermentation bin with balanced input and output to ferment the modified yeast to obtain a fermentation liquid. By measuring the total diester oil concentration and microbial concentration of the fermentation liquid, the unit microbial diester oil production can be calculated, thereby estimating the diester oil generation rate; measuring the biological waste concentration of the fermentation liquid, the microbial growth rate can be estimated, thereby estimating the base material consumption rate; due to the complete fermentation strategy of the present invention, the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time, thereby further limiting the diester oil generation rate, and finally obtaining the relationship between the diester oil generation rate and the comprehensive coefficient of the fermentation environment, the base material concentration and the base material injection speed, thereby experimentally obtaining the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed, and using them to guide the output of diester oil. Therefore, the present invention optimizes the fermentation process of diester oil based on sustainability and efficiency to improve overall production efficiency.

[0075] like Figure 2 , which is a functional module diagram of a diester oil microbial fermentation system based on fermentation process optimization provided by one embodiment of the present invention.

[0076] The diester oil microbial fermentation system 100 based on fermentation process optimization of the present invention can be installed in an electronic device. According to the functions to be implemented, the diester oil microbial fermentation system 100 based on fermentation process optimization can include a fermentation module 101, a diester oil concentration detection module 102, a substrate consumption rate calculation module 103, a diester oil generation rate calculation module 104 and a fermentation condition adjustment module 105. The module of the present invention can also be referred to as a unit, which refers to a series of computer program segments that can be executed by an electronic device processor and can complete fixed functions, which are stored in the memory of the electronic device.

[0077] The fermentation module 101 is used to obtain the modified yeast, and use the pre-constructed fermentation chamber to perform a fermentation operation on the modified yeast to obtain a fermentation liquid; the diester oil concentration detection module 102 is used to inject a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain the base material injection volume per unit time, and use the pre-constructed diaphragm to filter the fermentation liquid of the injection volume per unit time in the fermentation chamber to obtain a diester oil mixture, and identify the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can detect the biological production of the modified yeast. The modified yeast can be used to esterify the diester oil mixture to obtain synthetic diester oil, calculate the concentration of the synthetic diester oil according to the synthetic diester oil and the injection volume per unit time, and sum the diester oil concentration using the synthetic diester oil concentration to obtain the total diester oil concentration; the substrate consumption rate calculation module 103 is used to obtain the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation bin, calculate the fermentation time of the fermentation liquid according to the substrate injection speed and the fermentation volume, and monitor the cells in the fermentation liquid to obtain the microbial The diester oil production rate calculation module 104 is used to construct a microbial growth rate Monod equation using the base material concentration and the biowaste concentration, and to obtain the diester oil production rate according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production; the fermentation condition adjustment module 105 is used to adjust the diester oil production rate according to the preset complete fermentation strategy. The ratio of the generation rate to the base material consumption rate is equivalent to the fermentation time, and the fermentation complete restriction formula is obtained. According to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a diester oil generation rate change curve, and the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil generation rate is the maximum in the diester oil generation rate change curve are obtained. According to the optimal fermentation environment, optimal base material concentration and optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

[0078] In detail, each module in the diester oil microbial fermentation system 100 based on fermentation process optimization described in the embodiment of the present invention is used in the same manner as described above. Figure 1 The same technical means as the diester oil microbial fermentation method based on fermentation process optimization described in , and can produce the same technical effects, will not be repeated here.

[0079] like Figure 3 , which is a schematic diagram of the structure of an electronic device for implementing a diester oil microbial fermentation method based on fermentation process optimization provided by an embodiment of the present invention.

[0080] The electronic device 1 may include a processor 10, a memory 11 and a bus 12, and may also include a computer program stored in the memory 11 and executable on the processor 10, such as a diester oil microbial fermentation method program based on fermentation process optimization.

[0081] Wherein, the memory 11 includes at least one type of readable storage medium, and the readable storage medium includes flash memory, mobile hard disk, multimedia card, card-type memory (for example: SD or DX memory, etc.), magnetic memory, disk, optical disk, etc. In some embodiments, the memory 11 can be an internal storage unit of the electronic device 1, such as a mobile hard disk of the electronic device 1. In other embodiments, the memory 11 can also be an external storage device of the electronic device 1, such as a plug-in mobile hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (SecureDigital, SD) card, a flash card (Flash Card), etc. equipped on the electronic device 1. Further, the memory 11 also includes an internal storage unit of the electronic device 1 and an external storage device. The memory 11 can not only be used to store application software and various types of data installed in the electronic device 1, such as the code of the diester oil microbial fermentation method program based on fermentation process optimization, but also can be used to temporarily store data that has been output or is to be output.

[0082] The processor 10 may be composed of an integrated circuit in some embodiments, for example, a single packaged integrated circuit, or a plurality of packaged integrated circuits with the same or different functions, including one or more central processing units (CPUs), microprocessors, digital processing chips, graphics processors, and combinations of various control chips, etc. The processor 10 is the control core (Control Unit) of the electronic device, and uses various interfaces and lines to connect various components of the entire electronic device, and executes or executes programs or modules stored in the memory 11 (for example, a diester oil microbial fermentation method program based on fermentation process optimization, etc.), and calls data stored in the memory 11, so as to execute various functions of the electronic device 1 and process data.

[0083] The bus 12 may be a peripheral component interconnect (PCI) bus or an extended industry standard architecture (EISA) bus, etc. The bus 12 may be divided into an address bus, a data bus, a control bus, etc. The bus 12 is configured to realize connection and communication between the memory 11 and at least one processor 10, etc.

[0084] Figure 3 Only an electronic device with components is shown, and those skilled in the art will understand that Figure 3 The structure shown does not constitute a limitation on the electronic device 1, and may include fewer or more components than shown in the figure, or combine certain components, or arrange the components differently.

[0085] For example, although not shown, the electronic device 1 may also include a power source (such as a battery) for supplying power to various components. Preferably, the power source may be logically connected to the at least one processor 10 through a power management device, so that the power management device can realize functions such as charging management, discharging management, and power consumption management. The power source may also include one or more DC or AC power sources, recharging devices, power failure detection circuits, power converters or inverters, power status indicators, and other arbitrary components. The electronic device 1 may also include a variety of sensors, Bluetooth modules, Wi-Fi modules, etc., which will not be repeated here.

[0086] Furthermore, the electronic device 1 may also include a network interface. Optionally, the network interface may include a wired interface and / or a wireless interface (such as a WI-FI interface, a Bluetooth interface, etc.), which is generally used to establish a communication connection between the electronic device 1 and other electronic devices.

[0087] Optionally, the electronic device 1 may further include a user interface, which may be a display, an input unit (such as a keyboard), or a standard wired interface or a wireless interface. Optionally, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, and an OLED (Organic Light-Emitting Diode) touch device. The display may also be appropriately referred to as a display screen or a display unit, which is used to display information processed in the electronic device 1 and to display a visual user interface.

[0088] The diester oil microbial fermentation method program based on fermentation process optimization stored in the memory 11 of the electronic device 1 is a combination of multiple instructions. When running in the processor 10, it can achieve: obtaining modified yeast, fermenting the modified yeast using a pre-constructed fermentation chamber to obtain fermentation liquid; injecting a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and filtering the fermentation liquid of the unit time injection volume in the fermentation chamber using a pre-constructed diaphragm to obtain a diester oil mixture, and identifying the The diester oil concentration and the biological waste concentration in the diester oil mixture are calculated, wherein the diaphragm can pass the biological product of the modified yeast, but cannot pass the modified yeast; the diester oil mixture is esterified to obtain artificially synthesized diester oil, and the concentration of the artificially synthesized diester oil is calculated based on the artificially synthesized diester oil and the injection volume per unit time, and the diester oil concentration is summed up and calculated using the artificially synthesized diester oil concentration to obtain the total diester oil concentration; the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation bin are obtained, and the fermentation volume is calculated based on the injection speed of the base material and the fermentation volume. The fermentation time of the fermentation broth is determined; the cells in the fermentation broth are monitored to obtain the microbial concentration, and the unit microbial diester oil production is calculated according to the total diester oil concentration and the microbial concentration; the base material consumption rate is obtained according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration; the base material concentration and the biological waste concentration are used to construct the microbial growth rate Monod equation, and the diester oil generation rate is obtained according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production; the diester oil generation rate is calculated according to the preset complete fermentation strategy. The ratio of the fermentation rate to the base material consumption rate is equivalent to the fermentation time, and the fermentation complete restriction formula is obtained; according to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a diester oil generation rate change curve, and the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil generation rate is the maximum in the diester oil generation rate change curve are obtained; according to the optimal fermentation environment, optimal base material concentration and optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

[0089] Specifically, the specific implementation method of the processor 10 for the above instructions can refer to Figures 1 to 3 The description of the relevant steps in the corresponding embodiments will not be repeated here.

[0090] Furthermore, if the module / unit integrated in the electronic device 1 is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. The computer-readable storage medium can be volatile or non-volatile. For example, the computer-readable medium can include: any entity or device capable of carrying the computer program code, a recording medium, a USB flash drive, a mobile hard disk, a magnetic disk, an optical disk, a computer memory, and a read-only memory (ROM).

[0091] The present invention also provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor of an electronic device, the computer program can achieve: obtaining a modified yeast, and performing a fermentation operation on the modified yeast using a pre-constructed fermentation chamber to obtain a fermentation liquid; injecting a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and filtering the fermentation liquid of the unit time injection volume in the fermentation chamber using a pre-constructed diaphragm to obtain a diester oil mixture, and identifying the diester oil The concentration of diester oil and the concentration of biological waste in the mixture, wherein the diaphragm can pass the biological product of the modified yeast, but cannot pass the modified yeast; the diester oil mixture is esterified to obtain synthetic diester oil, the concentration of synthetic diester oil is calculated according to the synthetic diester oil and the injection volume per unit time, and the concentration of diester oil is summed up by the concentration of synthetic diester oil to obtain the total diester oil concentration; the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation bin are obtained, and the fermentation liquid is calculated according to the injection speed of the base material and the fermentation volume. fermentation time; monitoring the cells in the fermentation broth to obtain the microbial concentration, and calculating the unit microbial diester oil production according to the total diester oil concentration and the microbial concentration; obtaining the base material consumption rate according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration; constructing the microbial growth rate Monod equation using the base material concentration and the biological waste concentration, and obtaining the diester oil generation rate according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production; according to the preset complete fermentation strategy, the diester oil generation rate is compared with The ratio of the base material consumption rate is equivalent to the fermentation time, and the fermentation complete restriction formula is obtained; according to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, the base material concentration and the base material injection speed to obtain the diester oil generation rate change curve, and the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil generation rate is the maximum value in the diester oil generation rate change curve are obtained; according to the optimal fermentation environment, optimal base material concentration and optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

[0092] In the several embodiments provided by the present invention, it should be understood that the disclosed devices, systems and methods can be implemented in other ways. For example, the system embodiments described above are only illustrative, and actual implementation may have other division methods.

[0093] The modules described as separate components may or may not be physically separated, and the components shown as modules may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0094] In addition, each functional module in each embodiment of the present invention may be integrated into one processing unit, each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of hardware plus software functional modules.

[0095] It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A diester oil microbial fermentation method based on fermentation process optimization, characterized in that: The method comprises: obtaining a modified yeast, performing a fermentation operation on the modified yeast using a pre-constructed fermentation chamber to obtain a fermentation liquid; injecting a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and filtering the fermentation liquid of the unit time injection volume in the fermentation chamber using a pre-constructed diaphragm to obtain a diester oil mixture, and identifying the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can pass the biological products of the modified yeast but cannot pass the modified yeast; performing an esterification operation on the diester oil mixture to obtain an artificially synthesized diester oil, calculating the concentration of the artificially synthesized diester oil according to the artificially synthesized diester oil and the unit time injection volume, and summing the diester oil concentration using the artificially synthesized diester oil concentration to obtain a total diester oil concentration; obtaining the fermentation volume and the comprehensive coefficient of the fermentation environment of the fermentation chamber, and calculating the fermentation time of the fermentation liquid according to the base material injection speed and the fermentation volume; monitoring the cells in the fermentation liquid to obtain the microbial concentration , and according to the total diester oil concentration and the microbial concentration, the unit microbial diester oil production is calculated; according to the microbial concentration, the comprehensive coefficient of the fermentation environment, the biological waste concentration and the base material concentration, the base material consumption rate is obtained; using the base material concentration and the biological waste concentration, the Monod equation of the microbial growth rate is constructed, and according to the microbial concentration, the Monod equation of the microbial growth rate and the unit microbial diester oil production, the diester oil generation rate is obtained; according to the preset complete fermentation strategy, the ratio of the diester oil generation rate to the base material consumption rate is equivalent to the fermentation time to obtain the fermentation complete restriction formula; according to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, the base material concentration and the base material injection speed to obtain a diester oil generation rate change curve, and the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed when the diester oil generation rate is the maximum value in the diester oil generation rate change curve are obtained; according to the optimal fermentation environment, the optimal base material concentration and the optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

2. The diester oil microbial fermentation method based on fermentation process optimization according to claim 1, characterized in that: The method for obtaining the modified yeast comprises: obtaining an initial yeast, enhancing a metabolic pathway based on synthetic esters of the initial yeast, and obtaining a primary modified yeast; monitoring the ester synthesis activity of the primary modified yeast, screening the primary modified yeast with the highest ester synthesis activity, and obtaining a high-yield yeast; and breeding the high-yield yeast for a long term to obtain a plurality of breeding strains, and retaining a breeding strain with high ester synthesis stability among the plurality of breeding strains as the modified yeast.

3. The diester oil microbial fermentation method based on fermentation process optimization according to claim 2, characterized in that: The method of performing an esterification operation on the diester oil mixture to obtain an artificially synthesized diester oil comprises: filtering the diester oil in the diester oil mixture to obtain a bio-product mixture; adding a pre-constructed catalyst to the bio-product mixture and performing an esterification operation according to a preset esterification temperature to obtain an esterification solution; optically monitoring the esterification solution to obtain an infrared spectrum; and filtering the esterification solution to obtain an artificially synthesized diester oil when the absorption characteristic peak of the ester in the infrared spectrum no longer changes.

4. The diester oil microbial fermentation method based on fermentation process optimization according to claim 3, characterized in that: The fermentation time is expressed as: , where represents the fermentation time, represents the fermentation volume, Indicates the base material injection speed.

5. The diester oil microbial fermentation method based on fermentation process optimization according to claim 4, characterized in that: The method of obtaining the base material consumption rate according to the microbial concentration, the fermentation environment comprehensive coefficient, the biological waste concentration and the base material concentration includes: obtaining the growth inhibition constant of the biological waste concentration for the modified yeast, and performing weighted calculation on the biological waste concentration and the growth inhibition constant to obtain the growth inhibition coefficient; performing weighted calculation on the fermentation environment comprehensive coefficient and the base material concentration to obtain the growth promotion coefficient; calculating the difference between the growth promotion coefficient and the growth inhibition coefficient to obtain the growth comprehensive coefficient; performing weighted calculation on the microbial concentration according to the growth comprehensive coefficient to obtain the base material consumption rate, wherein the base material consumption rate is expressed as: , where represents the base material consumption rate, Indicates the concentration of the base material, represents the comprehensive coefficient of the fermentation environment, including temperature, oxygen concentration and pH value, represents the concentration of the microorganism, represents the biowaste concentration, It represents the growth inhibition constant of the modified yeast by the concentration of the biological waste.

6. The diester oil microbial fermentation method based on fermentation process optimization according to claim 5, characterized in that: The method of constructing a Monod equation for microbial growth rate by using the base material concentration and the biological waste concentration includes: obtaining the maximum growth rate of the modified yeast when the base material concentration is sufficient, and obtaining the base material concentration corresponding to the half-value speed of the maximum growth rate to obtain a promoting half-saturation constant of the modified yeast; obtaining an inhibiting half-saturation constant of the biological waste concentration for the modified yeast, performing weighted calculation on the inhibiting half-saturation constant and the biological waste concentration to obtain a growth inhibiting influence value, and summing the promoting half-saturation constant and the growth inhibiting influence value to obtain a corrected half-saturation constant; according to a pre-constructed Monod algorithm, the base material concentration, the maximum growth rate and the corrected half-saturation constant are used to calculate the growth rate of the modified yeast to obtain a Monod equation for microbial growth rate, and the Monod equation for microbial growth rate is expressed as: , where represents the growth rate of the modified yeast, It indicates the maximum growth rate of the modified yeast when the base material concentration is sufficient. represents the half-saturation constant of the base material concentration promoting the modified yeast, The inhibition half-saturation constant representing the effect of the biological waste concentration on the growth of the modified yeast.

7. The diester oil microbial fermentation method based on fermentation process optimization according to claim 6, characterized in that: The method of obtaining the diester oil generation rate according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production comprises: performing weighted calculation on the microbial concentration and the microbial growth rate Monod equation to obtain the microbial concentration change rate; performing weighted calculation on the microbial concentration change rate according to the unit microbial diester oil production to obtain the diester oil generation rate, wherein the diester oil generation rate is expressed as: , where represents the diester oil production rate, It represents the unit microbial diester oil production.

8. The diester oil microbial fermentation method based on fermentation process optimization according to claim 7, characterized in that: The complete fermentation limitation formula is expressed as: 。 9. The diester oil microbial fermentation method based on fermentation process optimization according to claim 8, characterized in that: The method of obtaining the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil generation rate in the diester oil generation rate change curve is at the maximum value comprises: spatially mapping the diester oil generation rate change curve in a three-dimensional space constructed according to the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a three-dimensional diester oil generation rate matrix; identifying the cluster center points in the three-dimensional diester oil generation rate matrix according to a pre-constructed K-means clustering algorithm, and obtaining the values ​​of the cluster center points in each dimension of the three-dimensional space to obtain the optimal fermentation environment, optimal base material concentration and optimal base material injection speed.

10. A diester oil microbial fermentation system based on fermentation process optimization, characterized in that: The system comprises: a fermentation module, which is used to obtain modified yeast, and use a pre-constructed fermentation chamber to perform a fermentation operation on the modified yeast to obtain a fermentation liquid; a diester oil concentration detection module, which is used to inject a base material of a preset base material concentration into the fermentation chamber according to a preset base material injection speed to obtain a unit time injection volume of the base material, and use a pre-constructed diaphragm to filter the fermentation liquid of the unit time injection volume in the fermentation chamber to obtain a diester oil mixture, and identify the diester oil concentration and the biological waste concentration in the diester oil mixture, wherein the diaphragm can detect the biological waste concentration of the modified yeast. The product can be produced by the modified yeast, and the diester oil mixture is esterified to obtain an artificially synthesized diester oil. The concentration of the artificially synthesized diester oil is calculated according to the artificially synthesized diester oil and the injection volume per unit time, and the diester oil concentration is summed up by using the artificially synthesized diester oil concentration to obtain a total diester oil concentration; a substrate consumption rate calculation module is used to obtain the fermentation volume of the fermentation bin and the comprehensive coefficient of the fermentation environment, calculate the fermentation time of the fermentation liquid according to the substrate injection speed and the fermentation volume, and monitor the cells in the fermentation liquid to obtain the microbial a diester oil generation rate calculation module, for constructing a microbial growth rate Monod equation using the base material concentration and the biowaste concentration, and obtaining a diester oil generation rate according to the microbial concentration, the microbial growth rate Monod equation and the unit microbial diester oil production; a fermentation condition adjustment module, for adjusting the diester oil generation rate and the microbial diester oil production according to a preset complete fermentation strategy; The ratio of the base material consumption rate is equivalent to the fermentation time, and the fermentation complete restriction formula is obtained. According to the fermentation complete restriction formula, the fermentation bin is subjected to a random adjustment simulation test operation based on the fermentation environment comprehensive coefficient, base material concentration and base material injection speed to obtain a diester oil generation rate change curve, and the optimal fermentation environment, optimal base material concentration and optimal base material injection speed when the diester oil generation rate is the maximum value in the diester oil generation rate change curve are obtained. According to the optimal fermentation environment, optimal base material concentration and optimal base material injection speed, the fermentation bin is continuously injected to obtain a stable diester oil output.

Images