Reagent and method for detecting carbapenemase type bacteria in enterobacter bacteria
By using a combination of Class A and Class B carbapenemase inhibitors, the problem of limited detection methods for different carbapenemase-type bacteria in Enterobacteria bacteria was solved, and a fast, accurate and low-cost detection method was achieved, which was suitable for clinical use.
Patent Information
- Application Number
- CN202510150450.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-11
- Publication Date
- 2025-04-11
AI Technical Summary
In the prior art, the detection methods of different carbapenemase-type bacteria in Enterobacteria bacteria are limited, the operation steps are complex, and the cost is high, making it difficult to meet the needs of rapid and accurate clinical screening.
The combination of Class A carbapenemase inhibitor R1 solution and Class B metalloenzyme inhibitor R2 solution is used to identify different carbapenemase-type strains by limiting the ratio of the two, providing a simple, easy-to-read and low-cost detection method.
It has achieved rapid and accurate typing of carbapenemase-type bacteria in Enterobacteria bacteria. It has simple operation, easy to read results, low cost, suitable for clinical use, and improved the CRE treatment level.
Smart Images
Figure CN119799841A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of bacteria detection, and in particular to a reagent and method for detecting carbapenemase-type bacteria in Enterobacteriales bacteria. Background Art
[0002] Currently, bacterial resistance has become a major challenge in the field of global public health, among which the infection caused by carbapenem-resistant Enterobacterales (CRE) is the most serious. Carbapenem antibiotics include imipenem, meropenem and ertapenem, which are one of the most effective antibiotics for treating infections caused by multidrug-resistant Gram-negative bacteria.
[0003] With the widespread clinical application of this type of drug, the resistance rate of Enterobacteriales to carbapenem antibiotics has been increasing rapidly year by year. Since CRE strains usually also carry genes that are resistant to other antibiotics, and strains of different enzyme-producing types have different sensitivities to limited antibiotics, the antibiotics available for clinical use are very limited.
[0004] Therefore, the rapid and accurate screening of enzyme-producing strains has important reference value for the selection of drugs for clinical anti-infection treatment. Therefore, the clinic can select effective treatment plans based on the typing of carbapenemase to improve the level of CRE treatment. In view of this, the present invention is specially proposed. Summary of the invention
[0005] In order to overcome the shortcomings of limited detection methods and complicated operation steps for different carbapenemase-type bacteria in Enterobacteriales, the present invention provides a reagent and method for detecting carbapenemase-type bacteria in Enterobacteriales. The detection reagent can not only realize the typing of strains with different enzyme types, but also has simple operation, easy-to-read results, low cost, and is very suitable for clinical use in carbapenemase-type bacteria detection.
[0006] A reagent for detecting carbapenemase-producing bacteria in Enterobacteriales bacteria comprises a Class A carbapenemase inhibitor R1 solution and a Class B metalloenzyme inhibitor R2 solution; the concentration of the Class A carbapenemase inhibitor R1 solution is 40-70 mg / mL; the concentration of the Class B metalloenzyme inhibitor R2 solution is 0.4-0.6 mol / L.
[0007] Preferably, the Class A carbapenemase inhibitor R1 solution is a 3-aminophenylboronic acid solution.
[0008] Preferably, the B-type metalloenzyme inhibitor R2 solution is an EDTA solution.
[0009] A method for detecting carbapenemase-producing bacteria in Enterobacteriales bacteria comprises the following steps: Step 1: Use a bacterial sampler to streak the pure test bacteria on a blood agar plate, incubate for 18-24 hours, and prepare a class A carbapenemase inhibitor R1 solution and a class B metalloenzyme inhibitor R2 solution respectively; Step 2: Use a bacterial sampler to pick fresh colonies, prepare a bacterial suspension, apply it on the surface of MHA agar, and after drying, use four areas of imipenem paper to stick them on the surface of MHA agar coated with the bacterial solution, and record them as four areas A, B, C, and D respectively; Step 3, adding the Class A carbapenemase inhibitor R1 solution obtained in step 1 to the imipenem paper disc B in step 2, adding the Class B metalloenzyme inhibitor R2 solution obtained in step 1 to the imipenem paper disc C, and adding the Class A carbapenemase inhibitor R1 solution and the Class B metalloenzyme inhibitor R2 solution obtained in step 1 to the imipenem paper disc D at the same time, wherein the volume ratio of the Class A carbapenemase inhibitor R1 solution to the Class B metalloenzyme inhibitor R2 solution is 1:1; Step 4: After incubation for 18-24 hours, read the diameter of the inhibition zone of imipenem, observe and compare the diameters of the inhibition zones of the four imipenem paper sheets A, B, C, and D, and obtain the results.
[0010] Preferably, a bacteria sampler comprises a gun body, a ceramic heat preservation chamber, an air supply pipe, a micro air pump, a storage battery, a fixed cylinder, a sliding block, a marking rod, a windproof lighter and a flamethrower; the gun body is fixedly connected with a ceramic heat preservation chamber; a fixed cylinder is slidably connected between the gun body and the ceramic heat preservation chamber; a first compression spring is fixedly connected between the fixed cylinder and the gun body; a sliding block is slidably connected to the gun body; the sliding block is fixedly connected to the fixed cylinder; a marking rod is fixedly connected to the fixed cylinder; a windproof lighter is installed in the gun body; the outlet end of the windproof lighter is connected with Flame-throwing tube; the outlet ends of the flame-throwing tubes are connected to the ceramic insulation cabin; a lighter control button for controlling the windproof lighter switch is provided on the gun body; a micro air pump is installed in the gun body; an air supply pipe connected to the ceramic insulation cabin is fixedly connected to the gun body, and the air supply pipe is aligned with the micro air pump; an air pump control button for controlling the micro air pump switch is provided in the gun body; a battery electrically connected to the micro air pump through a line is installed in the gun body; an air inlet groove structure connected to the ceramic insulation cabin is provided on the fixed cylinder; and no less than three air jet structures facing the marking rod are provided on the fixed cylinder.
[0011] Preferably, a filter cotton is fixedly connected to one side of the air inlet of the micro air pump in the gun body.
[0012] Preferably, the outlet end of the air supply pipe is aligned between the outlet ends of the two flame spray pipes.
[0013] Preferably, a miniature infrared thermometer for detecting the temperature toward the marking rod is installed on the gun body, and an indicator light is installed on the miniature infrared thermometer; the miniature infrared thermometer is electrically connected to the battery through a circuit.
[0014] Preferably, the marking rod is a hollow rod structure, and a liquid injection port structure is provided on the marking rod; a liquid storage cylinder storing the inhibitor solution is fixedly connected in the fixed cylinder; the marking rod is connected to the liquid storage cylinder; and a pull rod piston is slidably connected in the liquid storage cylinder.
[0015] Preferably, the outlet end of the liquid injection port structure of the marking rod is arranged to be tilted downward.
[0016] The present invention provides a reagent and method for detecting carbapenemase-type bacteria in Enterobacteriaceae. By using ethylenediaminetetraacetic acid compounds and triaminophenylboronic acid to match and limit the ratio of the two, strains producing class A, class B, and class A and class B carbapenemase are identified, providing a method for simply detecting and distinguishing carbapenemase in Enterobacteriaceae. Not only can the typing of carbapenemase be achieved, but the operation is simple, the results are easy to read, the cost is low, and it is very suitable for clinical use in Enterobacteriaceae carbapenemase phenotype detection. In addition, a method for A bacterial sampler used in a method for detecting carbapenemase-type bacteria in Enterobacteriales bacteria not only utilizes a ceramic insulation chamber and a windproof lighter to perform high-temperature sterilization on a marking rod, but also utilizes them to sterilize an airflow, thereby improving the cooling efficiency of the marking rod while reducing the residual amount of foreign bacteria on the marking rod. The marking rod can also cooperate with a liquid storage cylinder and a pull rod piston to form a liquid collection component, and the ceramic insulation chamber and the windproof lighter can also simultaneously perform high-temperature sterilization on the liquid collection component, thereby greatly reducing the interference of foreign bacteria in the detection of carbapenemase-type bacteria. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 A schematic diagram describing the addition of drug-sensitive tablets to the culture medium of the present invention; Figure 2 A schematic diagram describing the results of the Class A carbapenemase producing strain of the present invention; Figure 3 A schematic diagram describing the results of the Class B carbapenemase producing strain of the present invention; Figure 4 A schematic diagram describing the results of the strains producing class A and B carbapenemase of the present invention; Figure 5 A schematic perspective view of a bacteria sampler of the present invention is described; Figure 6 A cross-sectional view of a gun body of a bacteria sampler of the present invention is described; Figure 7 A cross-sectional view of a ceramic insulation chamber of a bacteria sampler of the present invention is described; Figure 8 A cross-sectional view of a fixed barrel of a bacteria sampler of the present invention is described; Fig. 9 A cross-sectional view of a streaking rod of a bacteria sampler of the present invention is described.
[0018] Figure numerals: 1-gun body, 11-ceramic insulation cabin, 12-air supply pipe, 13-mini air pump, 131-air pump control button, 14-filter cotton, 15-battery, 21-fixed cylinder, 2101-air inlet groove structure, 2102-jet nozzle structure, 22-first compression spring, 23-sliding block, 31-marking rod, 3101-liquid injection port structure, 32-liquid storage cylinder, 33-pull rod piston, 41-windproof lighter, 42-flame spray tube, 43-lighter control button, 44-second compression spring, 5-mini infrared thermometer. DETAILED DESCRIPTION
[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example
[0020] A reagent for detecting carbapenemase-producing bacteria in Enterobacteriales bacteria comprises a Class A carbapenemase inhibitor R1 solution and a Class B metalloenzyme inhibitor R2 solution; the Class A carbapenemase inhibitor R1 solution is a 3-aminophenylboronic acid solution with a concentration of 50 mg / mL; the Class B metalloenzyme inhibitor R2 solution is an EDTA solution with a concentration of 0.5 mol / L.
[0021] A method for detecting carbapenemase-producing bacteria in Enterobacteriales bacteria comprises the following steps: Step 1: Use a bacterial sampler to streak the pure test bacteria on a blood agar plate, incubate for 18-24 hours, and prepare a class A carbapenemase inhibitor R1 solution and a class B metalloenzyme inhibitor R2 solution respectively; Step 2: Use a bacterial sampler to pick fresh colonies, prepare a bacterial suspension, apply it on the surface of MHA agar, and after drying, Figure 1 As shown, four regions of imipenem paper were pasted on the surface of MHA agar coated with bacterial solution, and were marked as A, B, C, and D respectively; Step three, such as Figure 1 As shown, the Class A carbapenemase inhibitor R1 solution obtained in step 1 is added to the imipenem paper disc B in step 2, the Class B metalloenzyme inhibitor R2 solution obtained in step 1 is added to the imipenem paper disc C, and the Class A carbapenemase inhibitor R1 solution and the Class B metalloenzyme inhibitor R2 solution obtained in step 1 are added to the imipenem paper disc D at the same time, and the volume ratio of the Class A carbapenemase inhibitor R1 solution to the Class B metalloenzyme inhibitor R2 solution is 1:1; Step 4: After incubation for 18-24 hours, read the diameter of the inhibition zone of imipenem, observe and compare the diameters of the inhibition zones of the four imipenem paper sheets A, B, C, and D, and obtain the following results: Figure 2-Figure 4 shown.
[0022] The experiment was conducted by taking the above R1 solution having a concentration of 50 mg / mL and the R2 solution having a concentration of 0.5 mol / L as an example.
[0023] Day 1: Streak the pure test bacteria onto blood agar plates and incubate at 35℃±2℃ for 18-24h; The next day: Pick fresh pure colonies from overnight culture, prepare 0.5 McFarland turbidity bacterial suspension with 2 mL of physiological saline, and apply it on the MHA agar surface within 15 minutes. After drying for 15 minutes, use sterile tweezers to take out 4 imipenem paper discs and stick them on the agar surface coated with bacterial solution at positions A, B, C, and D. Figure 1 As shown; Add 5 μL of R1 solution on paper piece B, add 5 μL of R2 solution on paper piece C, and add 5 μL of R1 solution and 5 μL of R2 solution on paper piece D; After incubation at 35℃±2℃ for 18-24h, read the diameter of the inhibition zone of imipenem, and observe and compare the diameters of the inhibition zones of the four imipenem paper sheets A, B, C, and D. Results: The diameter of the inhibition zone of paper disc B minus the diameter of the inhibition zone of paper disc A ≥ 5 mm. Figure 2 As shown, indicating the production of class A carbapenemase; The diameter of the antibacterial zone of paper C - the diameter of the antibacterial zone of paper A ≥ 5 mm, such as Figure 3 As shown, it indicates the production of class B metalloenzymes; The diameter of the antibacterial circle of paper piece D minus the diameter of the antibacterial circle of paper piece A is ≥5mm, and the diameter of the antibacterial circle of paper piece B minus the diameter of the antibacterial circle of paper piece A is <5mm, and the diameter of the antibacterial circle of paper piece C minus the diameter of the antibacterial circle of paper piece A is <5mm. Figure 4 As shown, it indicates that class A carbapenemases and class B metalloenzymes are produced simultaneously.
[0024] A bacterial sampler is suitable for the bacterial sampling work in step one and step two of a method for detecting carbapenemase-type bacteria in Enterobacteriaceae. Figure 5-Figure 9As shown, it includes a gun body 1, a ceramic heat preservation chamber 11, an air supply pipe 12, a micro air pump 13, a battery 15, a fixed cylinder 21, a sliding block 23, a marking rod 31, a windproof lighter 41 and a flamethrower 42; the gun body 1 is fixedly connected with the ceramic heat preservation chamber 11; the gun body 1 and the ceramic heat preservation chamber 11 are slidably connected with the fixed cylinder 21; the fixed cylinder 21 and the gun body 1 are fixedly connected with a first compression spring 22; the gun body 1 is slidably connected with the sliding block 23; the sliding block 23 is fixedly connected to the fixed cylinder 21; The gun body 1 is provided with a second compression spring 44 fixedly connected to the lighter control button 43 and the gun body 1. A micro air pump 13 is installed inside the gun body 1; an air supply pipe 12 connected to the ceramic insulation cabin 11 is fixedly connected to the gun body 1, and the inlet end of the air supply pipe 12 is aligned with the air outlet of the micro air pump 13; an air pump control button 131 for controlling the switch of the micro air pump 13 is provided in the gun body 1, and the air pump control button 131 can be kept in the on state by only a short press; the outlet end of the air supply pipe 12 is aligned between the outlet ends of the two flame-spraying tubes 42, so that the air flow blown out from the air supply pipe 12 continues to flow through the two flame-spraying tubes 42 The flame is ejected, so that the airflow directly contacts the high-temperature flame, thereby improving the sterilization effect of the airflow; a filter cotton 14 is fixedly connected to the gun body 1, and the filter cotton 14 is located on the air inlet side of the micro air pump 13; a battery 15 is installed in the gun body 1, and the battery 15 is electrically connected to the micro air pump 13 through a line to power it; an air inlet groove structure 2101 connected to the ceramic insulation cabin 11 is opened on the fixed cylinder 21; three air jet structures 2102 facing the marking rod 31 are opened on the fixed cylinder 21.
[0025] like Figure 4 As shown, a miniature infrared thermometer 5 is installed on the gun body 1, and an indicator light is installed on the miniature infrared thermometer 5; the miniature infrared thermometer 5 is electrically connected to the battery 15 through a circuit, and the battery 15 continuously supplies power to the miniature infrared thermometer 5; the miniature infrared thermometer 5 continuously detects the temperature of the marking rod 31, and when the miniature infrared thermometer 5 detects that the temperature of the marking rod 31 exceeds a specified value, the indicator light on the miniature infrared thermometer 5 will remain on, and only when the miniature infrared thermometer 5 detects that the temperature of the marking rod 31 drops below a specified value, the indicator light on the miniature infrared thermometer 5 will go out, so as to remind the user whether the temperature of the current marking rod 31 has dropped to a standard state.
[0026] The steps of high-temperature sterilization and disinfection treatment of a bacteria sampler of the present invention are as follows: first, the user holds the gun body 1 with one hand and presses the lighter control button 43 to compress the second compression spring 44, which can trigger the windproof lighter 41 to spray flames into the ceramic insulation cabin 11 through the flamethrower 42. The windproof lighter 41 mainly uses technologies such as piezoelectric ceramic igniter and re-ignition catalytic net to prevent wind from extinguishing fire, which is a widely used existing technology. Therefore, compared with ordinary lighters, the windproof lighter 41 can maintain stable combustion under strong airflow. At the same time, the user pulls the sliding block 23 with the other hand to drive the fixed cylinder 21 to move to the right along the gun body 1, and the fixed cylinder 21 drives the first compression spring 22 to compress, and the fixed cylinder 21 drives The marking rod 31 moves to the right, allowing various areas of the marking rod 31 to pass through the ceramic insulation chamber 11 in turn and come into contact with the flame ejected from the flame tube 42, thereby achieving high-temperature sterilization and disinfection of the fixed cylinder 21 and the marking rod 31. Then the user slowly releases the sliding block 23, and the compressed first compression spring 22 pushes the fixed cylinder 21 along the gun body 1 to drive the marking rod 31 to reset to the left. During this process, the user keeps pressing the lighter control button 43, allowing the windproof lighter 41 to continue to spray flames to the marking rod 31 and the fixed cylinder 21 through the flame tube 42, thereby extending the high-temperature sterilization and disinfection time of the marking rod 31 and the fixed cylinder 21, and improving the high-temperature sterilization and disinfection effect of the marking rod 31 and the fixed cylinder 21.
[0027] After the high-temperature sterilization treatment of the marking rod 31 and the fixed tube 21 is completed, the user continues to press the lighter control button 43, allowing the windproof lighter 41 to continuously spray flames toward the marking rod 31 and the fixed tube 21 through the flame-spraying tube 42, thereby increasing the temperature of the fixed tube 21 and the ceramic heat-insulating chamber 11. At the same time, the user presses the air pump control button 131 to start the micro air pump 13, and the micro air pump 13 continuously pumps the outside air through the filter cotton 14 into the air duct 12 to form a continuous airflow. After the airflow is blown out from the air duct 12, it passes through the high-temperature jet flame and sequentially contacts The inner wall of the ceramic insulation chamber 11 and the surface of the air duct 12 allow the air flow to continue to complete the sterilization treatment in a high-temperature environment. After the sterilization treatment, the air flow enters the fixed cylinder 21 through the air inlet groove structure 2101, and then blows out through the various jet outlet structures 2102 of the fixed cylinder 21 to flow through the surface of the marking rod 31. The marking rod 31 that has completed the high-temperature sterilization treatment is air-cooled by the continuously flowing air flow until the micro infrared thermometer 5 detects that the surface temperature of the marking rod 31 has cooled to below forty-six degrees Celsius. The marking rod 31 that has completed the cooling treatment can be used for sampling.
[0028] Since the marking rod 31 is sterilized and disinfected at high temperature and cooled by the airflow after the sterilization, the residual foreign bacteria on the surface of the marking rod 31 can be greatly reduced, thereby reducing the interference of foreign bacteria in the detection of carbapenemase-type bacteria.
[0029] The marking rod 31 is configured as a hollow rod structure, and an injection port structure 3101 is provided at one end of the marking rod 31 away from the gun body 1; a liquid storage cylinder 32 storing an inhibitor solution is fixedly connected in the fixed cylinder 21; the end of the marking rod 31 away from the injection port structure 3101 is connected to the liquid storage cylinder 32; a pull rod piston 33 is slidably connected in the liquid storage cylinder 32; the outlet end of the injection port structure 3101 of the marking rod 31 is configured to be in a downwardly inclined state.
[0030] The marking rod 31, the liquid storage cylinder 32 and the rod piston 33 together form a liquid collection component. The flame sprayed from the flame-spraying tube 42 to the fixed cylinder 21 and the marking rod 31 can perform high-temperature sterilization and disinfection on the liquid collection component in real time. When the prepared inhibitor solution needs to be added to the surface of the MHA agar, the user first holds the gun body 1 with one hand to immerse the injection port structure 3101 of the marking rod 31 into the inhibitor solution, and then pulls the rod piston 33 with the other hand to move it outward along the inside of the liquid storage cylinder 32, generating a suction negative pressure inside the liquid storage cylinder 32, so that the inhibitor solution is drawn into the liquid storage through the marking rod 31. The cylinder 32 is then moved by the user to align the injection port structure 3101 of the marking rod 31 downwardly with the MHA agar surface, and then the pull rod piston 33 is pressed to allow the pull rod piston 33 to push the inhibitor solution inside the liquid storage cylinder 32 to the MHA agar surface. At the same time, the user presses the sliding block 23 on the fixed cylinder 21 with his thumb to avoid pushing the fixed cylinder 21 to move toward the inside of the gun body 1 during the pressing of the pull rod piston 33, thereby completing the addition of the inhibitor solution to the MHA agar surface. This is suitable for the addition of the inhibitor solution in step three of a method for detecting carbapenemase-type bacteria in Enterobacteriaceae.
[0031] The above description is only an example of the present invention and is not intended to limit the present invention. Any equivalent substitutions made within the principles of the present invention should be included in the protection scope of the present invention. The contents not elaborated in detail in the present invention belong to the existing technologies known to those skilled in the art.
Claims
1. A reagent for detecting carbapenemase-producing bacteria in Enterobacteriaceae, characterized in that: It includes a class A carbapenemase inhibitor R1 solution and a class B metalloenzyme inhibitor R2 solution; the concentration of the class A carbapenemase inhibitor R1 solution is 40-70 mg / mL; the concentration of the class B metalloenzyme inhibitor R2 solution is 0.4-0.6 mol / L.
2. A reagent for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 1, characterized in that: The class A carbapenemase inhibitor R1 solution is a 3-aminophenylboronic acid solution.
3. The reagent for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 1, characterized in that: The Class B metalloenzyme inhibitor R2 solution is an EDTA solution.
4. A method for detecting carbapenemase-producing bacteria in Enterobacteriaceae, the method using a reagent for detecting carbapenemase-producing bacteria in Enterobacteriaceae according to any one of claims 1 to 3, characterized in that: The following steps are included: Step 1: Use a bacterial sampler to streak the pure test bacteria on a blood agar plate, incubate for 18-24 hours, and prepare a class A carbapenemase inhibitor R1 solution and a class B metalloenzyme inhibitor R2 solution respectively; Step 2: Use a bacterial sampler to pick fresh bacterial colonies, prepare a bacterial suspension, apply it on the surface of MHA agar, and after drying, use four imipenem paper sheets to stick them on the surface of MHA agar coated with the bacterial suspension, and mark them as four areas A, B, C, and D respectively; Step 3, adding the Class A carbapenemase inhibitor R1 solution obtained in step 1 to the imipenem paper disc B in step 2, adding the Class B metalloenzyme inhibitor R2 solution obtained in step 1 to the imipenem paper disc C, and adding the Class A carbapenemase inhibitor R1 solution and the Class B metalloenzyme inhibitor R2 solution obtained in step 1 to the imipenem paper disc D at the same time, wherein the volume ratio of the Class A carbapenemase inhibitor R1 solution to the Class B metalloenzyme inhibitor R2 solution is 1:1; Step 4: After incubation for 18-24 hours, read the diameter of the inhibition zone of imipenem, observe and compare the diameters of the inhibition zones of the imipenem paper disks in the four areas A, B, C, and D, and obtain the results.
5. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 4, characterized in that: The bacterial sampler used comprises a gun body (1); a ceramic heat preservation chamber (11) is fixedly connected inside the gun body (1); a fixed tube (21) is slidably connected between the gun body (1) and the ceramic heat preservation chamber (11); a first compression spring (22) is fixedly connected between the fixed tube (21) and the gun body (1); a sliding block (23) is slidably connected inside the gun body (1); the sliding block (23) is fixedly connected to the fixed tube (21); a marking rod (31) is fixedly connected to the fixed tube (21); a windproof lighter (41) is installed inside the gun body (1); the outlet end of the windproof lighter (41) is connected to a flamethrower (42); the outlet end of the flamethrower (42) is connected to the ceramic heat preservation chamber (11); the gun body (1) is provided with a plurality of airtight ... A lighter control button (43) for controlling the switch of the windproof lighter (41) is provided; a micro air pump (13) is installed in the gun body (1); an air supply pipe (12) connected to the ceramic heat preservation chamber (11) is fixedly connected to the gun body (1), and the air supply pipe (12) is aligned with the micro air pump (13); an air pump control button (131) for controlling the switch of the micro air pump (13) is provided in the gun body (1); a battery (15) electrically connected to the micro air pump (13) through a circuit is installed in the gun body (1); an air inlet groove structure (2101) connected to the ceramic heat preservation chamber (11) is provided on the fixed cylinder (21); and no less than three air jet structures (2102) facing the marking rod (31) are provided on the fixed cylinder (21).
6. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 5, characterized in that: A filter cotton (14) is fixedly connected to one side of the air inlet of the micro air pump (13) in the gun body (1).
7. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 5, characterized in that: The outlet end of the air delivery pipe (12) is aligned between the outlet ends of the two flame spraying pipes (42).
8. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 5, characterized in that: A miniature infrared thermometer (5) for detecting the temperature of the marking rod (31) is mounted on the gun body (1), and an indicator light is mounted on the miniature infrared thermometer (5); the miniature infrared thermometer (5) is electrically connected to a storage battery (15) via a circuit.
9. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 5, characterized in that: The marking rod (31) is configured as a hollow rod structure, and a liquid injection port structure (3101) is provided on the marking rod (31); a liquid storage cylinder (32) storing an inhibitor solution is fixedly connected in the fixed cylinder (21); the marking rod (31) is connected to the liquid storage cylinder (32); and a pull rod piston (33) is slidably connected in the liquid storage cylinder (32).
10. The method for detecting carbapenemase-producing bacteria in Enterobacteriales according to claim 9, characterized in that: The outlet end of the liquid injection port structure (3101) of the marking rod (31) is arranged in a downwardly inclined state.