Full-automatic online monitoring device and method for drugs in water

Abstract

The invention discloses a full-automatic online monitoring device and a full-automatic online monitoring method for drugs in water, and aims to solve the problem that the detection cost and the time cost are too high in the traditional sewage poison detection process. According to the device, the whole process from collection, filtration, solid-phase extraction, nitrogen blowing, redissolution to fluorescence detection of a sewage sample is realized through an automatic process, and the monitoring efficiency and convenience are remarkably improved. According to the method, through continuous and automatic operation steps, manual intervention is reduced, operation errors are reduced, rapid and accurate detection of drugs in sewage is achieved, the detection result can be uploaded to the cloud end in time, and efficient and accurate technical support is provided for drug banning management and scientific research. Besides, the device design considers the capability of long-time continuous work, can realize real-time collection and analysis of waste liquid in remote areas, improves the monitoring continuity and coverage range, reduces the monitoring cost, and enhances the scientificity and effectiveness of monitoring.

Description

Technical Field

[0001] The invention relates to a fully automatic on-line monitoring device for drugs in water, and also to a corresponding on-line monitoring method for drugs in water, belonging to the technical field of analysis and testing. Background Art

[0002] Sewage drug testing is an important means of drug monitoring and assessment. It can accurately track areas where drugs are rampant, and therefore has received widespread attention from drug control agencies and scientific research organizations around the world. However, sewage drug testing still faces challenges in practical applications. The main problem is that the testing cost and time cost are too high. The current practice is to use automatic sampling equipment to collect sewage samples, and then send the samples to the laboratory for analysis. This process not only increases the economic cost of testing, but also prolongs the testing time, and it is impossible to upload the test results immediately.

[0003] China's utility model patent CN212301009U discloses a water sample on-site freezing extraction instrument for sewage toxicity testing. This technical solution improves the integration of the extractor by integrating the sampling components inside the box, reduces the number of equipment required for on-site sampling, and facilitates sample collection in different regions. In addition, the device is also equipped with a temperature control module to achieve low-temperature storage of samples.

[0004] Although this on-site freezing extraction instrument for water samples has made some improvements in sampling and sample preservation, since the collected samples still need to be sent to professional identification agencies for testing, it does not fundamentally solve the problems of high testing costs and long testing times. Summary of the invention

[0005] The primary technical problem to be solved by the present invention is to provide a fully automatic on-line monitoring device for drugs in water.

[0006] Another technical problem to be solved by the present invention is to provide a fully automatic online monitoring method for drugs in water quality.

[0007] In order to achieve the above technical objectives, the present invention adopts the following technical solutions:

[0008] According to a first aspect of an embodiment of the present invention, there is provided a fully automatic online monitoring device for drugs in water quality, comprising: a housing, an electric pump, a liquid inlet pipe, a waste liquid pipe, a filter, a solid phase extraction column turntable, a reconstitution tray, a nitrogen module, a reconstitution tank, a water purification tank, a pipetting module, a card slot box, a fluorescence detection module and a control module;

[0009] Wherein, the liquid inlet and the waste liquid outlet are both provided on the shell and are connected to the liquid inlet pipe and the waste liquid pipe respectively;

[0010] The solid phase extraction column turntable is fixed to the inside of the housing by the first rotating shaft and is located above the reconstitution tray, and includes n solid phase extraction columns arranged in a ring on the circular periphery of the solid phase extraction column turntable; the reconstitution tray is fixed to the inside of the housing by the second rotating shaft, and includes m reconstitution test tubes arranged in a ring on the circular periphery of the reconstitution tray, and the position of the reconstitution test tubes away from the first rotating shaft is the first position;

[0011] The filter is connected to the liquid inlet through the liquid inlet pipe and to the waste liquid outlet through the waste liquid pipe, and is located directly above the solid phase extraction column turntable and aligned with at least one of the solid phase extraction columns in the vertical direction;

[0012] The card slot box includes z reagent cards, the card outlet of the card slot box is aligned with the card inlet of the fluorescence detection module in the horizontal direction, and the position of the card outlet of the card slot box is the second position;

[0013] The liquid transfer module is arranged on one side of the solid phase extraction column turntable, the reconstitution tray, the nitrogen module, the reconstitution tank, and the clean water tank, and can move horizontally between the first position and the second position;

[0014] The nitrogen module, the rehydration solution tank and the clean water tank are located between the first position and the second position;

[0015] The control module is connected to the filter, the first rotating shaft, the second rotating shaft, the nitrogen module, the pipetting module, the card slot box, the fluorescence detection module and the electric pump through a data line;

[0016] Among them, n, m, and z are all positive integers.

[0017] Preferably, the nitrogen module comprises a nitrogen tank, a nitrogen pipe and a nitrogen nozzle; wherein the nitrogen pipe is used to store nitrogen, the nitrogen pipe is used to connect the nitrogen tank and the nitrogen nozzle, and the nitrogen nozzle is used to spray nitrogen into the extract in the solid phase extraction column to perform solid phase extraction.

[0018] Preferably, the pipetting module includes a horizontal slide, a horizontal slide, a vertical slide, a pipetting arm and a pipetting dropper; wherein, the length of the horizontal slide enables the horizontal slide to move horizontally between the first position and the second position; the horizontal slide is installed on the horizontal slide; the vertical slide is vertically fixed to the horizontal slide in the up and down directions; one end of the pipetting arm is connected to the vertical slide, so that the pipetting arm can move vertically on the vertical slide, and the other end of the pipetting arm is connected to the pipetting dropper; the pipetting dropper is parallel to the vertical slide, so that it can enter and exit the clean water tank, the re-dissolving solution tank and the re-dissolving test tube in the up and down directions, and the pipetting dropper is used to absorb the re-dissolving solution and re-dissolve the sample to be tested.

[0019] Preferably, the control module includes a processor and a memory; wherein the memory is coupled to the processor and is used to store a computer program.

[0020] Preferably, the control module further includes a wireless communication unit; wherein the wireless communication unit is coupled to the processor and is used to upload a concentration detection report to the cloud.

[0021] Preferably, the first rotating axis and the second rotating axis are designed to be arranged in parallel, with a spacing greater than the radius of the reconstitution tray and smaller than the sum of the radius of the solid phase extraction column turntable and the radius of the reconstitution tray, so that the solid phase extraction column and the reconstitution test tube can be accurately docked.

[0022] Preferably, the solid phase extraction column and the reconstitution test tube can overlap in the vertical direction.

[0023] Preferably, a portion of the filtered waste liquid directly drips into the solid phase extraction column aligned with the filter due to gravity, and the other portion is discharged through the waste liquid port.

[0024] According to a second aspect of an embodiment of the present invention, there is provided a fully automatic online monitoring method for drugs in water, comprising the following steps:

[0025] S1: Collect the original solution to be tested, filter the original solution to be tested through a filter, and obtain the filtered solution to be tested;

[0026] S2: collecting the filtered test liquid into a solid phase extraction column, adding an eluent thereto, purifying and eluting the filtered test liquid to obtain a purified test liquid;

[0027] S3: nitrogen blowing the purified test liquid to obtain a nitrogen-blown test sample;

[0028] S4: Immerse the pipette dropper in a clean water tank for cleaning;

[0029] S5: After cleaning, immerse the pipette into the reconstitution solution tank to absorb the reconstitution solution, and add the reconstitution solution to the sample to be tested after nitrogen blowing to reconstitute it, so as to obtain a reconstituted sample to be tested;

[0030] S6: Pipet the reconstituted sample to be tested and drop the reconstituted sample to be tested into two reagent cards respectively;

[0031] S7: Put the two reagent cards into the fluorescence detection module in turn, perform fluorescence detection on the reconstituted sample to be tested according to the preset detection items, and after obtaining the respective detection results, take the average of the two detection results and generate a concentration curve;

[0032] S8: Generate a concentration detection report according to the concentration curve.

[0033] Preferably, in step S1, the interval between collection of the test stock solution is greater than or equal to 2 hours.

[0034] Compared with the prior art, the present invention realizes the whole process from sewage sample collection, filtration, solid phase extraction, nitrogen blowing, re-dissolution to fluorescence detection through an automated process, solving the problem of high detection cost and time cost in the traditional sewage drug testing process. The present invention reduces manual intervention and operating errors through continuous automated operation steps, realizes rapid and accurate detection of drugs in sewage, and can upload the test results to the cloud in real time, providing efficient and accurate technical support for drug control management and scientific research. In addition, the design of the device takes into account the ability to work continuously for a long time, and can realize real-time collection and analysis of waste liquid in remote areas, improve the continuity and coverage of monitoring, reduce monitoring costs, and enhance the scientificity and effectiveness of monitoring. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 A schematic diagram of the structure of a fully automatic online monitoring device for drugs in water provided by an embodiment of the present invention;

[0036] Figure 2 A sample schematic diagram of a fully automatic online monitoring device for drugs in water provided by an embodiment of the present invention;

[0037] Figure 3 This is a schematic diagram of the structure of a control module in an embodiment of the present invention;

[0038] Figure 4 A flow chart of a fully automatic on-line monitoring method for drugs in water provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0039] The technical content of the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0040] First embodiment

[0041] like Figure 1 As shown, the first embodiment of the present invention provides a fully automatic online monitoring device for drugs in water, comprising: a housing, an electric pump, a liquid inlet pipe, a waste liquid pipe, a filter, a solid phase extraction column turntable, a reconstitution tray, a nitrogen module, a reconstitution tank, a water purification tank, a liquid transfer module, a card slot box, a fluorescence detection module and a control module. The control module is used to control the operation of each component.

[0042] like Figure 2 As shown, the liquid inlet and the waste liquid outlet are respectively arranged on the outer shell of the device, and they are connected to the liquid inlet pipe and the waste liquid pipe. Such a design allows the control module to automatically control the inflow and outflow of waste liquid: the waste liquid to be tested is introduced through the liquid inlet, and after analysis and processing, the waste liquid is discharged through the waste liquid outlet. This process realizes the automatic management of waste liquid and improves the efficiency and convenience of the monitoring process.

[0043] The solid phase extraction column turntable and the reconstitution tray arranged inside the device are the key parts for realizing sample processing. The solid phase extraction column turntable is fixed inside the shell by the first rotating shaft and is located directly above the reconstitution tray. The turntable is designed with n solid phase extraction columns, which are arranged in a ring along the circular circumference of the turntable, where n is a positive integer. The reconstitution tray is fixed inside the shell by the second rotating shaft, and is also designed with m reconstitution test tubes, which are also arranged in a ring along the circular circumference of the tray, where m is also a positive integer. The reconstitution test tubes are arranged in a manner so that they form a first position on the side away from the first rotating shaft.

[0044] In order to ensure that the SPE column and the reconstitution tube can be accurately docked, the first rotating axis and the second rotating axis are designed to be parallel. Their spacing is carefully designed to be larger than the radius of the reconstitution tray, and smaller than the sum of the radius of the SPE column turntable and the radius of the reconstitution tray. This design allows one or more SPE columns to be precisely aligned with one or more reconstitution tubes in the vertical direction, that is, they can overlap in the upper and lower directions. This precise alignment mechanism is crucial for the automated sample processing process, which ensures that the sample can be seamlessly transferred between the SPE and reconstitution steps.

[0045] The filter plays a vital role in the present invention. It is connected to the liquid inlet through the liquid inlet pipe and to the waste liquid outlet through the waste liquid pipe, and is placed directly above the solid phase extraction column turntable. This layout allows the waste liquid from the liquid inlet pipe to be filtered through the filter under the action of an electric pump (not shown in the figure). A portion of the filtered waste liquid will drip directly into the solid phase extraction column aligned with the filter due to gravity, while most of the remaining waste liquid will be discharged through the waste liquid outlet. This design allows the waste liquid to be effectively distributed to each solid phase extraction column.

[0046] Furthermore, the first rotating shaft on the SPE column turntable is responsible for controlling the positioning of the SPE columns. When a SPE column is filled with filtered waste liquid, the first rotating shaft automatically rotates to align the next SPE column with the filter at an angle of 360° divided by the number of SPE columns (n). Such a rotation pattern is carefully designed to ensure that the time for the waste liquid to drip from the filter and fill the SPE column matches the rotation period of the first rotating shaft, thereby achieving an efficient and continuous filtration and distribution process.

[0047] In addition, the rotation mode of the first rotating shaft and the second rotating shaft is also carefully designed to ensure that the filtered waste liquid enters from the top of the solid phase extraction column and flows out from the bottom after a certain period of time T, while the second rotating shaft rotates 360° divided by the number of reconstitution test tubes (m). Such synchronous rotation ensures that at least one reconstitution test tube is always aligned with one solid phase extraction column up and down, or two reconstitution test tubes are aligned with two solid phase extraction columns respectively. This precise alignment and synchronous rotation mechanism ensures the continuity and automation of the waste liquid treatment and sample preparation process.

[0048] The card slot box is designed to store z reagent cards, where z is the liquid inlet and waste liquid port respectively set on the outer shell of the device, which are connected to the liquid inlet pipe and the waste liquid pipe. This design allows the control module to automatically control the inflow and outflow of waste liquid: the waste liquid to be tested is introduced through the liquid inlet, and after analysis and processing, the waste liquid is discharged through the waste liquid port. This process realizes the automatic management of waste liquid and improves the efficiency and convenience of the monitoring process.

[0049] The pipetting module is a key component in the device, which is placed on one side of the solid phase extraction column turntable, the reconstitution tray, the nitrogen module, the reconstitution tank and the clean water tank. The module can move horizontally between the first position and the second position, so that the pipette dropper can easily enter the clean water tank, the reconstitution tank and the reconstitution test tube. This design allows the pipetting module to flexibly transfer liquids between different containers and test tubes, improving the efficiency and accuracy of the operation.

[0050] The nitrogen module, reconstitution tank, and clean water tank are arranged between the first and second positions. This layout is designed to optimize space utilization and ensure that the pipetting module can easily access these components. This layout helps to realize automated liquid handling processes, reducing the need for manual operation and the possibility of errors during operation.

[0051] The control module is the brain of the entire device. It is connected to the filter, the first rotating shaft, the second rotating shaft, the nitrogen module, the pipetting module, the card slot box, the fluorescence detection module, the electric pump and other parts through data lines. This connection method allows the control module to accurately control the work of each component to ensure the automation and coordination of the entire monitoring process. Through centralized control, operators can easily monitor and adjust the entire monitoring process.

[0052] In one embodiment of the present invention, the solid phase extraction column turntable is designed with a maximum of 30 solid phase extraction columns. According to the frequency of automatically collecting the test liquid every 2 hours, the device can work continuously for 60 hours to achieve uninterrupted sample collection. This design enables real-time collection and analysis of waste liquid in remote areas, which not only makes drug monitoring work in these areas a reality, but also significantly increases the monitoring area that can be covered by a single monitoring personnel, thereby improving the efficiency and scope of monitoring.

[0053] Solid phase extraction column is a sample pretreatment technology. It uses solid particle chromatographic fillers (usually installed in small column-type devices) to chemically separate different components of the sample. It is an efficient separation tool based on the principle of liquid chromatography separation. The solid phase extraction column uses chemical adsorption, ion exchange, hydrogen bonding and van der Waals forces to enrich the target molecules from the sample to a certain adsorbent. These adsorbent materials (such as bonded silica gel and polymers) are filled inside the solid phase extraction column. When the sample enters the solid phase extraction column, the target compound will interact with the adsorbent and be retained in the column, while other components will be excluded. Subsequently, an appropriate solvent is used for elution to separate the target molecules from the column to achieve the purpose of separation, enrichment and purification.

[0054] In one embodiment of the present invention, the nitrogen module includes a nitrogen tank, a nitrogen pipe and a nitrogen nozzle, wherein the nitrogen pipe is used to store nitrogen, the nitrogen pipe is used to connect the nitrogen tank and the nitrogen nozzle, and the nitrogen nozzle is used to spray nitrogen into the extract in the solid phase extraction column to perform solid phase extraction.

[0055] Nitrogen is mainly used for sample concentration and drying. By passing nitrogen into the container to form an airflow, the solvent on the surface of the liquid reagent in the container evaporates, thereby achieving sample concentration or solvent removal. It is mainly used in the fields of pesticide residue analysis, environmental analysis, biological analysis, food quality inspection, pharmaceutical drug inspection and commodity inspection.

[0056] In one embodiment of the present invention, the reconstitution tank is used to store the reconstitution solution, and the function of the reconstitution solution is to dissolve the substance that has been in a dry state into the solvent again to form a new solution.

[0057] In one embodiment of the present invention, the clean water tank is used to store clean water. Because the pipette needs to continuously absorb the reconstitution solution and each sample to be tested, the pipette needs to be cleaned in the clean water tank before each absorption of the reconstitution solution.

[0058] In one embodiment of the present invention, the pipetting module includes a horizontal slide, a horizontal slide, a vertical slide, a pipetting arm and a pipetting dropper. The length of the horizontal slide enables the horizontal slide to move horizontally between a first position and a second position; the horizontal slide is mounted on the horizontal slide; the vertical slide is vertically fixed to the horizontal slide in the up and down directions; one end of the pipetting arm is connected to the vertical slide, so that the pipetting arm can move vertically on the vertical slide, and the other end of the pipetting arm is connected to the pipetting dropper; the pipetting dropper is parallel to the vertical slide, so that it can enter and exit the clean water tank, the reconstitution tank and the reconstitution test tube in the up and down directions, and the pipetting dropper is used to absorb the reconstitution solution and reconstitute the sample to be tested.

[0059] The fluorescence detection module is a key component of the device, which is responsible for the final drug concentration detection of the reconstituted sample to be tested. The module realizes the automatic entry and exit of the reagent card by horizontally aligning the card outlet of the card slot box, wherein the card slot box contains multiple reagent cards for storing and managing the samples to be tested. In the operation process, after the liquid transfer module drips the reconstituted sample to be tested into the reagent card, the reagent card will be placed in the fluorescence detection module for detection in turn. The module analyzes the sample using fluorescence technology according to the preset detection items to obtain the respective test results. In order to ensure the accuracy of the results, the module will average the test results of the two reagent cards and generate a concentration curve. Finally, the fluorescence detection module will generate a detailed concentration detection report based on the concentration curve, providing accurate data support for drug control management and scientific research. Through this automated fluorescence detection process, the present invention not only improves the efficiency of drug monitoring, but also enhances the accuracy and reliability of the results.

[0060] In one embodiment of the present invention, the reconstitution tray is designed to contain up to 30 reconstitution tubes, and the card slot box is designed to contain up to 90 reagent cards. Such a design allows the device to process a large number of samples in an automated process, improving the throughput and efficiency of monitoring. The provision of the reconstitution tubes ensures that after solid phase extraction, each purified sample has enough space for the reconstitution step, and the number of reagent cards provides sufficient detection capacity for fluorescence detection. This high-capacity design enables the device to run for a long time without frequent manual intervention, especially in situations where continuous monitoring is required or where it is difficult to frequently replace consumables in remote areas. Such a design significantly improves the continuity and convenience of monitoring, while also reducing the complexity and cost of operation.

[0061] like Figure 3As shown, in one embodiment of the present invention, the control module includes one or more processors, a memory, and a wireless communication unit. Among them, the wireless communication unit is used to upload the concentration detection report to the cloud. The memory is used to store a computer program. When the computer program is executed by the processor, the online monitoring method for drugs in water quality provided by the embodiment of the present invention (described in detail below) can be implemented.

[0062] Among them, the processor is used to control the overall operation of the device to complete all or part of the steps of the above method. The processor can be a central processing unit (CPU), a graphics processing unit (GPU), a field programmable gate array (FPGA), an application-specific integrated circuit (ASIC), a digital signal processing (DSP) chip, etc. The memory is used to store various types of data to support the operation of the device, and these data may include, for example, instructions for any application or method for operating on the device, and application-related data. The memory can be implemented by any type of volatile or non-volatile storage device or a combination thereof, such as a static random access memory (SRAM), an electrically erasable programmable read-only memory (EEPROM), an erasable programmable read-only memory (EPROM), a programmable read-only memory (PROM), a read-only memory (ROM), a magnetic memory, a flash memory, etc.

[0063] Second embodiment

[0064] like Figure 4 As shown, the second embodiment of the present invention provides a fully automatic online monitoring method for drugs in water, which includes at least the following steps:

[0065] S1: Collect the original solution to be tested, filter the original solution to be tested through a filter, and obtain the filtered solution to be tested.

[0066] S2: collecting the filtered test liquid into a solid phase extraction column, adding an eluent thereto, purifying and eluting the filtered test liquid to obtain a purified test liquid.

[0067] S3: nitrogen blow the purified test liquid to obtain a nitrogen blown test sample.

[0068] S4: Immerse the pipette dropper in a clean water tank for cleaning.

[0069] S5: After cleaning, immerse the pipette into the reconstitution solution tank to absorb the reconstitution solution, and add the reconstitution solution to the sample to be tested after nitrogen blowing to reconstitute it, so as to obtain the reconstituted sample to be tested.

[0070] S6: Use a pipette to absorb the reconstituted sample to be tested, and drop the reconstituted sample to be tested into two reagent cards respectively.

[0071] S7: Put the two reagent cards into the fluorescence detection module in turn, perform fluorescence detection on the reconstituted sample to be tested according to the preset detection items, and after obtaining the respective test results, take the average of the two test results and generate a concentration curve.

[0072] S8: Generate a concentration detection report according to the concentration curve.

[0073] The method for online monitoring of drugs in water provided by the second embodiment of the present invention makes full use of the unique design of the above-mentioned device to realize an efficient and automated sewage drug monitoring process. Among them, through the setting of the liquid inlet pipe and the waste liquid pipe, the original liquid to be tested can be automatically collected, and the filtered liquid to be tested can be obtained after filtering through the filter. This step eliminates the tediousness of manual collection and filtering, reduces the human error and contamination risk in the sample processing process, and improves the efficiency and accuracy of sample processing.

[0074] Furthermore, the parallel setting and precise alignment mechanism of the solid phase extraction column turntable and the reconstitution tray enable the solid phase extraction and reconstitution steps to be seamlessly connected and completed automatically. The rotating shaft design of the solid phase extraction column turntable ensures that the waste liquid can be evenly distributed to each solid phase extraction column, while the rotation of the reconstitution tray ensures the precise connection between the reconstitution tube and the solid phase extraction column, realizing the automated transfer and processing of samples.

[0075] The nitrogen module automates the solid phase extraction process by injecting nitrogen into the extract in the solid phase extraction column, further improving the efficiency and purity of sample processing. This step plays an important role in reducing impurities in the sample and improving detection sensitivity.

[0076] Finally, by automatically dropping the processed sample into the reagent card and using fluorescence detection technology to quantitatively analyze the drug concentration, this method can quickly and accurately obtain the test results. This process not only reduces manual operations, but also improves the sensitivity and accuracy of drug detection, making sewage drug monitoring more efficient and reliable.

[0077] In one embodiment of the present invention, the collection frequency of the original solution to be tested in step S1 can be set according to the needs of the user, which provides flexibility to adapt to different monitoring needs and conditions. The set collection interval is greater than or equal to 2 hours. This design is based on the limited pressure bearing capacity of the solid phase extraction column. Therefore, during the solid phase extraction process, there is an upper limit to the setting of the air pressure value. This means that in order to ensure that the solid phase extraction column will not be damaged due to excessive pressure, the time required for the complete detection process is at least 2 hours. Such a design ensures the safety and reliability of the device during operation, while also taking into account the durability and maintenance cost of the solid phase extraction column.

[0078] In addition, in order to improve the accuracy of the test results, steps S7 to S8 can be repeated 5 times. This design of repeated detection helps to reduce accidental errors and improve the stability and credibility of the test results. By repeatedly detecting and averaging, the drug concentration in the sample can be more accurately evaluated, thereby providing more accurate data support for drug control management departments and scientific research institutions. This design embodies the present invention's emphasis on detection accuracy, ensures the scientificity and effectiveness of the monitoring results, helps to more accurately evaluate drug abuse, and provides data support for the formulation of relevant policies and measures.

[0079] It should be noted that the above embodiments are only examples, and the technical solutions of the various embodiments can be combined, all within the protection scope of the present invention.

[0080] It should be understood that the terms "upper", "lower", "horizontal", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0081] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0082] The above is a detailed description of the fully automatic online monitoring device and method for drugs in water provided by the present invention. For those skilled in the art, any obvious changes made to it without departing from the essence of the present invention will constitute an infringement of the patent right of the present invention and will bear corresponding legal responsibilities.

Claims

1. A fully automatic online monitoring device for drugs in water, characterized in that include: Housing, electric pump, liquid inlet pipe, waste liquid pipe, filter, solid phase extraction column turntable, reconstitution tray, nitrogen module, reconstitution tank, water purification tank, pipetting module, card slot box, fluorescence detection module and control module; Wherein, the liquid inlet and the waste liquid outlet are both provided on the shell and are connected to the liquid inlet pipe and the waste liquid pipe respectively; The solid phase extraction column turntable is fixed to the inside of the housing by the first rotating shaft and is located above the reconstitution tray, and includes n solid phase extraction columns arranged in a ring on the circular periphery of the solid phase extraction column turntable; the reconstitution tray is fixed to the inside of the housing by the second rotating shaft, and includes m reconstitution test tubes arranged in a ring on the circular periphery of the reconstitution tray, and the position of the reconstitution test tubes away from the first rotating shaft is the first position; The filter is connected to the liquid inlet through the liquid inlet pipe and to the waste liquid outlet through the waste liquid pipe, and is located directly above the solid phase extraction column turntable and aligned with at least one of the solid phase extraction columns in the vertical direction; The card slot box includes z reagent cards, the card outlet of the card slot box is aligned with the card inlet of the fluorescence detection module in the horizontal direction, and the position of the card outlet of the card slot box is the second position; The liquid transfer module is arranged on one side of the solid phase extraction column turntable, the reconstitution tray, the nitrogen module, the reconstitution tank, and the clean water tank, and can move horizontally between the first position and the second position; The nitrogen module, the rehydration solution tank and the clean water tank are located between the first position and the second position; The control module is connected to the filter, the first rotating shaft, the second rotating shaft, the nitrogen module, the pipetting module, the card slot box, the fluorescence detection module and the electric pump through a data line; Among them, n, m, and z are all positive integers.

2. The on-line monitoring device for drugs in water as claimed in claim 1 is characterized in that The nitrogen module comprises a nitrogen tank, a nitrogen pipe and a nitrogen nozzle; The nitrogen pipe is used to store nitrogen, the nitrogen pipe is used to connect the nitrogen tank with the nitrogen nozzle, and the nitrogen nozzle is used to spray nitrogen into the extraction liquid in the solid phase extraction column to perform solid phase extraction.

3. The on-line monitoring device for drugs in water as claimed in claim 1 is characterized in that The pipetting module includes a horizontal slide, a horizontal slide, a vertical slide, a pipetting arm and a pipetting dropper; wherein, the length of the horizontal slide enables the horizontal slide to move horizontally between the first position and the second position; the horizontal slide is installed on the horizontal slide; the vertical slide is vertically fixed to the horizontal slide in the up and down directions; one end of the pipetting arm is connected to the vertical slide, so that the pipetting arm can move vertically on the vertical slide, and the other end of the pipetting arm is connected to the pipetting dropper; the pipetting dropper is parallel to the vertical slide, so that it can enter and exit the clean water tank, the reconstitution solution tank and the reconstitution test tube in the up and down directions, and the pipetting dropper is used to absorb the reconstitution solution and reconstitute the sample to be tested.

4. The on-line monitoring device for drugs in water as claimed in claim 1 is characterized in that The control module includes a processor and a memory; wherein the memory is coupled to the processor and is used to store a computer program.

5. The on-line monitoring device for drugs in water as claimed in claim 4 is characterized in that The control module also includes a wireless communication unit; wherein the wireless communication unit is coupled to the processor and is used to upload a concentration detection report to the cloud.

6. The on-line monitoring device for drugs in water as claimed in claim 1, characterized in that The first rotating shaft and the second rotating shaft are designed to be arranged in parallel, with a spacing greater than the radius of the reconstitution tray and smaller than the sum of the radius of the solid phase extraction column turntable and the radius of the reconstitution tray, so that the solid phase extraction column and the reconstitution test tube can be accurately docked.

7. The on-line monitoring device for drugs in water as claimed in claim 6 is characterized in that The solid phase extraction column and the reconstitution test tube can overlap in the vertical direction.

8. The on-line monitoring device for drugs in water as claimed in claim 1 is characterized in that A portion of the filtered waste liquid directly drips into the solid phase extraction column aligned with the filter due to gravity, and the other portion is discharged through the waste liquid port.

9. A fully automatic method for online monitoring of drugs in water, based on the online monitoring device for drugs in water as claimed in any one of claims 1 to 8, characterized in that The steps include: S1: Collect the original solution to be tested, filter the original solution to be tested through a filter, and obtain the filtered solution to be tested; S2: collecting the filtered test liquid into a solid phase extraction column, adding an eluent thereto, purifying and eluting the filtered test liquid to obtain a purified test liquid; S3: nitrogen blowing the purified test liquid to obtain a nitrogen-blown test sample; S4: Immerse the pipette dropper in a clean water tank for cleaning; S5: After cleaning, immerse the pipette into the reconstitution solution tank to absorb the reconstitution solution, and add the reconstitution solution to the sample to be tested after nitrogen blowing to reconstitute it, so as to obtain a reconstituted sample to be tested; S6: Pipet the reconstituted sample to be tested and drop the reconstituted sample to be tested into two reagent cards respectively; S7: Put the two reagent cards into the fluorescence detection module in turn, perform fluorescence detection on the reconstituted sample to be tested according to the preset detection items, and after obtaining the respective detection results, take the average of the two detection results and generate a concentration curve; S8: Generate a concentration detection report according to the concentration curve.

10. The method for online monitoring of drugs in water according to claim 9, characterized in that: The interval time for collecting the raw solution to be tested in step S1 is greater than or equal to 2 hours.

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