Acoustic levitation microscopic testing device

Abstract

The invention discloses an acoustic levitation microscopic testing device, relates to the technical field of intelligent detection, and solves the problems that the manual slicing efficiency is low and real-time continuous observation cannot be realized. One end of a quantitative jet gun is connected with a support through a telescopic mechanical arm, and a reed is arranged on the quantitative jet gun; the jet head is horizontally arranged below the quantitative jet gun; the reflector is arranged in the middle of the support, and the sound wave generator is arranged under the reflector. A muzzle of the quantitative jet gun and an air outlet of the jet head are positioned between the reflector and the sound wave generator; the multiple cold needles are arranged on the support through the telescopic mechanical arm. The cold needle sends the collected sample into the kit, the kit is transferred into the image analysis system through the sample preparation device, and image analysis is carried out on the sample. According to the invention, the particles are stably conveyed by using acoustic suspension, the method has the characteristic of dynamic real-time continuous observation, and the activated sludge is analyzed layer by layer by using the cold needle sample treatment system, so that the analysis of the activated sludge particles can be realized.

Description

Technical Field

[0001] The invention relates to the technical field of intelligent detection, in particular to an acoustic levitation microscopic testing device. Background Art

[0002] The activated sludge method is the most commonly used sewage treatment method at home and abroad. The structure of activated sludge reflects the status of the sewage treatment system. Regular monitoring of the microscopic parameters of activated sludge flocs can provide important information for the dynamic changes of sewage treatment operation parameters. Therefore, it is necessary to analyze and observe the activated sludge flocs in different states.

[0003] However, the observation of activated sludge particles in most sewage treatment plants remains at the theoretical stage. Some large water plants require professionals to manually slice the activated sludge particles, which is affected by human factors and is time-consuming, labor-intensive, and inefficient. In addition, manual slicing can only achieve static observation of sampling first and then testing, and cannot achieve real-time continuous observation. Therefore, the results obtained are one-sided and incomplete. Summary of the invention

[0004] In order to solve the above-mentioned problems of low efficiency of manual sectioning and inability to achieve real-time continuous observation, the present invention proposes an acoustic levitation microscopic testing device. The present invention uses acoustic levitation to stably transport particles, has the characteristics of dynamic real-time continuous observation, and uses a cold needle sample processing system to analyze activated sludge layer by layer, which can realize the analysis of activated sludge particles; it can also be applied to the field of medical pathology sections, the decomposition of microbial cell structures in biology, and the monitoring of protozoa in ecology.

[0005] The present invention proposes an acoustic levitation microscopy testing device, which specifically includes a bracket, a quantitative sampling device, an acoustic levitation stabilization device, a protective gas propulsion device, a cold needle sample processing device, a sample preparation device and an image analysis system. The bracket is provided with a quantitative sampling device at one end, an acoustic levitation stabilization device is provided in the middle, and a cold needle sample processing device is provided at the other end; the quantitative sampling device includes a quantitative jet gun and a jet head, one end of the quantitative jet gun is connected to the bracket through a telescopic mechanical arm, and the other end of the quantitative jet gun is connected to the bracket through a reed; the jet head is connected to the bracket through a telescopic mechanical arm and is horizontally arranged below the quantitative jet gun, and the jet head is connected to the bracket through a telescopic mechanical arm. The air head is connected to the air pump; the acoustic suspension stabilization device includes a reflector and a sound wave generator, the reflector is arranged in the middle of the bracket, and the sound wave generator is arranged directly below the reflector; the muzzle of the quantitative jet gun and the air outlet of the jet head are located between the reflector and the sound wave generator; the cold needle sample processing device includes a plurality of cold needles, and the cold needles are arranged on the bracket through a telescopic mechanical arm; a sample preparation device and an image analysis system are arranged below the plurality of cold needles, and the sample preparation device includes a plurality of test kits; the cold needles send the collected samples into the test kits, and the test kits are transferred to the image analysis system through the sample preparation device to perform image analysis on the samples.

[0006] Furthermore, the acoustic levitation stabilization device also includes three automatic zoom microscopes and electromagnetic relays, the electromagnetic relay is arranged at the bottom of the sound wave generator; the automatic zoom microscope is arranged on the sound wave generator and is located between the sound wave generator and the reflector to observe the droplets dripped from the quantitative jet gun.

[0007] Furthermore, a light source compensation device is arranged on the bracket, and the light source compensation device provides a light source for the automatic zoom microscope device.

[0008] Furthermore, the cold needle includes a cold needle plate and a plurality of fine needles. The cold needle plate is a regular polygonal ring structure. Two fine needles are arranged on each edge of the cold needle plate, and a plurality of fine needles point to the center of the cold needle plate. The cold needle plate is arranged vertically, and the edge is connected to the telescopic robotic arm.

[0009] Furthermore, the side length of the cold needle plate decreases from close to the sound wave generator to away from the sound wave generator.

[0010] Furthermore, the bracket is provided with a cold needle stabilizing device and a slide rail, and the cold needle is slidably set on the bracket through a telescopic mechanical arm and a slide rail; the cold needle is extended through the telescopic mechanical arm and inserted into the cold needle stabilizing device, and the temperature of the cold needle is maintained by the low temperature environment inside the cold needle stabilizing device.

[0011] Furthermore, the sample preparation device also includes a robotic arm, a conveying device and a telescopic bracket, and the telescopic bracket is arranged below the conveying device; the robotic arm clamps the reagent kit and places it on the conveying device through the telescopic bracket.

[0012] Furthermore, a reagent kit storage box is provided at the end of the conveying device.

[0013] Furthermore, the image analysis system includes a microscope and a display, the microscope is arranged on one side of the transmission device, and the microscope and the display are connected.

[0014] Furthermore, the acoustic levitation microscopy testing device also includes a gas propulsion device, which includes a plurality of jet heads. The plurality of jet heads of the gas propulsion device are arranged in the middle and at the end between the reflector and the sound wave generator to control the balance of the droplets.

[0015] The beneficial effects of the acoustic levitation microscopy testing device described in the present invention are:

[0016] (1) The acoustic levitation microscopy testing device described in the present invention overcomes the problems of low efficiency of manual sectioning and inability to achieve real-time continuous observation. Liquid droplets are suspended by an acoustic wave generator and a reflector and contacted with a number of cold needles under the push of an airflow. The cold needles peel off the droplets and send the samples into a reagent kit. The samples in the reagent kit are observed through a microscope. The entire process does not require manual sectioning, which effectively improves efficiency. Continuous observation can be achieved by continuously dripping droplets with a quantitative jet gun. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings constituting a part of this application are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0018] In the attached picture:

[0019] Figure 1 It is a structural schematic diagram of an acoustic levitation microscopy testing device according to the present invention;

[0020] Figure 2 It is a schematic diagram of the setting of an automatic zoom microscope device of an acoustic levitation microscope testing device according to the present invention;

[0021] Figure 3 It is a structural schematic diagram of a quantitative jet gun of an acoustic levitation microscopy testing device according to the present invention;

[0022] Figure 4 It is a schematic diagram of the structure of a cold needle of an acoustic levitation microscopy testing device according to the present invention;

[0023] Figure 5 It is a structural schematic diagram of a kit for an acoustic levitation microscopy testing device according to the present invention;

[0024] Among them: 1-air pump, 2-jet head, 3-quantitative jet gun, 4-reflector, 5-telescopic mechanical arm, 6-cold needle, 7-automatic zoom microscope, 8-sound wave generator, 9-mechanical arm, 10-cold needle stabilization device, 11-electromagnetic relay, 12-microscope, 13-display, 14-light source compensation device, 15-transmission device, 16-telescopic bracket, 17-reagent box storage box, 18-reed, 19-reagent box, 20-bracket, 21-slide rail. DETAILED DESCRIPTION

[0025] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0027] In the description of the invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the invention can be understood according to specific circumstances.

[0028] In addition, the technical features involved in different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0029] Specific implementation method 1: See Figure 1-Figure 5The present embodiment is described in detail. The acoustic levitation microscopy testing device described in the present embodiment specifically includes a bracket 20, a quantitative sampling device, an acoustic levitation stabilization device, a cold needle sample processing device, a sample preparation device and an image analysis system. The bracket 20 is provided with a quantitative sampling device at one end, an acoustic levitation stabilization device is provided in the middle, and a cold needle sample processing device is provided at the other end; the quantitative sampling device includes a quantitative jet gun 3 and a nozzle 2, the quantitative jet gun 3 is provided on the bracket 20, and one end of the quantitative jet gun 3 is connected to the bracket 20 through a telescopic mechanical arm 5, and the telescopic mechanical arm 5 can control the quantitative jet gun 3 to move, and the rotation radius is 25cm-30cm; the quantitative jet gun 3 is also provided with a reed 18, and the reed 18 is connected to the bracket 20; the reed 18 is provided with a tensioning device, and the tension of the reed 18 is controlled by the tensioning device, so that different vibration amplitudes can be generated; the telescopic mechanical arm 5 is used to apply a fixed force to the quantitative jet gun 3 and the reed 18 is moved at about 100mm. The quantitative jet gun 3 is vibrated under the beam, so that the sample droplets drip from the muzzle of the quantitative jet gun 3; the jet head 2 is connected to the bracket 20 through a telescopic mechanical arm 5 and is horizontally arranged below the quantitative jet gun 3, and the jet head 2 is connected to the air pump 1; the acoustic suspension stabilization device includes a reflector 4 and a sound wave generator 8, the reflector 4 is arranged in the middle of the bracket 20, and the sound wave generator 8 is arranged directly below the reflector 4, the sound wave generator 8 is used to send sound waves, and the generator 4 is used to receive sound waves to form a sound field; the muzzle of the quantitative jet gun 3 and the air outlet of the jet head 2 are located between the reflector 4 and the sound wave generator 8, when the sample is dripped out of the gun head of the quantitative jet gun 3, the sample droplets can be suspended in the sound field under the action of the reflector 4 and the sound wave generator 8; the cold needle sample processing device includes a plurality of cold needles 6, which are arranged on the bracket 20 through a telescopic mechanical arm 5; after the sample drips out, it moves in the sound field under the action of the gas ejected from the jet head 2 until it contacts the cold needle 6.

[0030] A sample preparation device and an image analysis system are arranged below the several cold needles 6. The sample preparation device includes several test kits 19. The test kit 19 is a semi-solid agar culture medium test kit. The test kit 19 is 25 cm in length and 10 cm in width. The edges of both sides of the test kit 19 are provided with magnets. Several partitions are arranged inside the test kit 19. The cold needle 6 sends the collected samples into the test kit 19. The test kit 19 is transferred to the image analysis system through the sample preparation device to perform image analysis on the samples.

[0031] The acoustic levitation stabilization device also includes three automatic zoom microscopes 7 and an electromagnetic relay 11. The electromagnetic relay 11 is arranged at the bottom of the sound wave generator 8, and the electromagnetic relay 11 controls the sound wave generator 8; the automatic zoom microscope 7 is arranged on the sound wave generator 8 and is between the sound wave generator 8 and the reflector 4. The three automatic zoom microscopes 7 are evenly arranged around the circumference, and a preliminary visual judgment is made on the droplets dripped from the quantitative jet gun 3 from three different angles.

[0032] The bracket 20 is provided with a light source compensation device 14, which provides light source for the automatic zoom microscope 7. The light source compensation device 14 is an LED light source.

[0033] The cold needle 6 includes a cold needle plate and a plurality of fine needles. The cold needle plate is a regular polygonal ring structure. In the present embodiment, the cold needle plate is a regular hexagonal ring structure. Two fine needles are arranged on each side of the cold needle plate, and a plurality of fine needles point to the center of the cold needle plate. The cold needle plate is arranged vertically, and the cold needle plates of the plurality of cold needles 6 are arranged coaxially, and the edge of the cold needle plate is connected to the telescopic robotic arm 5.

[0034] The side length of the cold needle plate decreases from close to the sound wave generator 8 to far away from the sound wave generator 8, that is, the cold needle plate of the cold needle 6 close to the sound wave generator 8 is larger, and the cold needle plate of the cold needle 6 far away from the sound wave generator 8 is smaller. In this embodiment, the side lengths of the cold needle plate are 10mm, 8mm and 6mm respectively. The fine needles on different cold needles 6 are of the same length, so that the circular structure formed by the end of the fine needle in the center of the cold needle 6 gradually decreases away from the sound wave generator 8.

[0035] The bracket 20 is provided with a cold needle stabilizing device 10 and a slide rail 21, the cold needle 6 is connected to one end of the telescopic mechanical arm 5, and the other end of the telescopic mechanical arm 5 is connected to the slide rail 21, so that a plurality of cold needles 6 are slidably arranged on the bracket 20; the interior of the cold needle stabilizing device 10 is a low-temperature environment, which is achieved by setting dry ice or continuously cooling the interior of the cold needle stabilizing device 10 through a heat exchange medium; after the cold needle 6 completes a sample collection, it moves to the top of the cold needle stabilizing device 10 under the action of the slide rail 21, the telescopic mechanical arm 5 is extended, and the cold needle 6 is inserted into the cold needle stabilizing device 10, and the cold needle 6 is cooled by the low-temperature environment inside the cold needle stabilizing device 10 to maintain the temperature of the cold needle 6 stable.

[0036] The sample preparation device further includes a robotic arm 9, a conveyor 15 and a telescopic bracket 16, wherein the telescopic bracket 16 is disposed below the conveyor 15; the robotic arm 9 clamps the reagent kit 19 and places it on the conveyor 15 through the telescopic bracket 16. The conveyor 15 is 2 meters long and 1 meter wide, and has a running speed range of 0.1 mm / s to 0.5 mm / s.

[0037] A reagent kit storage box 17 is disposed at the end of the conveying device 15 .

[0038] The image analysis system includes a microscope 12 and a display 13. The microscope 12 is arranged on one side of the conveying device 15. The microscope 12 and the display 13 are connected. The microscope 12 performs partition monitoring on the samples in the reagent kit 19 and displays the results on the display 13. The display 13 can automatically record into video or image data through operation, and then perform deep analysis, classification and other operations through deep learning and other algorithms to form a large number of data sets, which provide a basis for subsequent test verification. The obtained high-precision model can bring results for subsequent monitoring.

[0039] The acoustic levitation microscopy test device also includes a gas propulsion device, which includes a plurality of jet heads 2. The plurality of jet heads 2 of the gas propulsion device are arranged in the middle and at the end between the reflector 4 and the sound wave generator 8 to control the balance of the droplets. The plurality of jet heads 2 of the gas propulsion device are arranged obliquely; the plurality of jet heads 2 of the gas propulsion device are divided into two parts, one part is arranged on the bracket 20, and the other part is arranged on the ground. The two parts of the jet heads 2 are opposite to each other up and down, and the angle between the gas outlet direction of the two parts of the jet heads 2 and the horizontal plane is in the range of 30°-45°, so as to control the parallel movement of the droplets, such as Figure 1 shown.

[0040] The specific working process of the acoustic levitation microscopy testing device described in the present invention is as follows:

[0041] Before sampling, press and hold the top button of the quantitative jet gun 3 to immerse the gun head of the quantitative jet gun 3 in the liquid. When sampling, slowly release the top button by manipulating the telescopic mechanical arm 5 to further transfer the gun head to the sound field of the acoustic suspension device. The droplet size can be determined by the range of the quantitative jet gun, so the volume range of the droplet is 100-1000 microliters; after the gun head moves into the sound field, the telescopic mechanical arm 5 is set to apply a fixed stress to the quantitative jet gun 3, so that the magnitude of the stress can be controlled when dripping the sample, thereby controlling the number of samples dripped by the quantitative jet gun, and the sample droplets are dripped into the sound field through the cooperation of the telescopic mechanical arm 5 and the reed 18.

[0042] After the sample droplets fall into the sound field between the reflector 4 and the sound wave generator 8, the horizontally arranged jet heads 2 provide moving power for the sample droplets. During the horizontal movement, the sample droplets pass through the automatic zoom microscope 7, and the automatic zoom microscope 7 performs a preliminary visual judgment on the sample droplets. Afterwards, several tilted jet heads 2 of the gas propulsion device adjust the positions of the sample droplets in the sound field, control the parallel movement of the droplets, and ensure that the sample droplets can contact the cold needle 6.

[0043] When the sample droplet passes through the cold needle 6, due to the low temperature, the dissection is realized layer by layer, so the peeled liquid will adhere to the cold needle 6 to form a sample. The cold needle plate is pulled horizontally by the telescopic mechanical arm 5 to expand the hexagon into a rectangle; then the telescopic mechanical arm 5 is pressed down to make it penetrate into the culture medium of the reagent kit 19 clamped by the mechanical arm 9 to complete the sample processing.

[0044] After the sample processing is completed, the telescopic bracket 16 rises and passes through the conveying device 15. At this time, the conveying device 15 stops running, and the robotic arm 9 places the reagent kit 19 on the telescopic bracket 16; the telescopic bracket 16 descends and places the reagent kit 19 on the conveying device 15. The reagent kit 19 is adsorbed on the conveying device 15 by the magnetic force on the edges on both sides; the conveying device 15 is further controlled to transport the reagent kit 19 to the microscope 12 for partition monitoring, and the observed picture will be transmitted to the display 13. The display 13 can automatically record into video or image data through operation, and then perform deep analysis, classification and other operations through deep learning and other algorithms to form a large number of data sets, which provide a basis for subsequent test verification. The obtained high-precision model can bring results for subsequent monitoring; after the observation is completed, the reagent kit 19 will fall into the reagent kit storage box 17 at the end of the conveying device 15.

[0045] To summarize the above implementation cases, the acoustic levitation microscopy testing device described in the present invention overcomes the problems of low efficiency of manual slicing and inability to achieve real-time continuous observation. The droplets are suspended by the acoustic wave generator 8 and the reflector 4 and contacted with a plurality of cold needles 6 under the push of the airflow. The cold needles 6 peel off the droplets and send the samples into the reagent kit 19. The samples in the reagent kit 19 are observed through the microscope 12. The whole process does not require manual participation in slicing, which effectively improves the efficiency. The purpose of continuous observation can be achieved by continuously dripping droplets through the quantitative jet gun 3.

[0046] The specific embodiments described above further illustrate the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the invention. It can also be a reasonable combination of the features recorded in the above implementation methods. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An acoustic levitation microscopy testing device, characterized in that: The invention comprises a support (20), a quantitative injection device, an acoustic suspension stabilization device, a cold needle sample processing device, a sample preparation device and an image analysis system. The support (20) is provided with a quantitative injection device at one end, an acoustic suspension stabilization device is provided at the middle, and a cold needle sample processing device is provided at the other end. The quantitative injection device comprises a quantitative jet gun (3) and a jet head (2). One end of the quantitative jet gun (3) is connected to the support (20) via a telescopic mechanical arm (5), and the other end of the quantitative jet gun (3) is connected to the support (20) via a reed (18). The jet head (2) is connected to the support (20) via a telescopic mechanical arm (5). The device is connected to a support (20) and is horizontally arranged below a quantitative jet gun (3), and the jet head (2) is connected to an air pump (1); the acoustic suspension stabilization device comprises a reflector (4) and a sound wave generator (8), the reflector (4) is arranged in the middle of the support (20), and the sound wave generator (8) is arranged directly below the reflector (4); the muzzle of the quantitative jet gun (3) and the air outlet of the jet head (2) are located between the reflector (4) and the sound wave generator (8); the cold needle sample processing device comprises a plurality of cold needles (6), and the cold needles (6) are arranged on the support (20) through a telescopic mechanical arm (5); A sample preparation device and an image analysis system are arranged below the plurality of cold needles (6), and the sample preparation device includes a plurality of reagent kits (19); the cold needles (6) send the collected samples into the reagent kits (19), and the reagent kits (19) are transferred to the image analysis system through the sample preparation device, and image analysis is performed on the samples.

2. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The acoustic suspension stabilization device also includes three automatic zoom microscopes (7) and an electromagnetic relay (11), wherein the electromagnetic relay (11) is arranged at the bottom of the acoustic wave generator (8); the automatic zoom microscope (7) is arranged on the acoustic wave generator (8) and between the acoustic wave generator (8) and the reflector (4) to observe the droplets dripped from the quantitative jet gun (3).

3. The acoustic levitation microscopy testing device according to claim 2, characterized in that: The support (20) is provided with a light source compensation device (14), and the light source compensation device (14) provides a light source for the automatic zoom microscope device (7).

4. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The cold needle (6) comprises a cold needle plate and a plurality of fine needles. The cold needle plate is a regular polygonal ring structure. Two fine needles are arranged on each side of the cold needle plate, and the plurality of fine needles point to the center of the cold needle plate. The cold needle plate is arranged vertically, and the edge is connected to the telescopic mechanical arm (5).

5. The acoustic levitation microscopy testing device according to claim 4, characterized in that: The side length of the cold needle plate decreases from a direction close to the sound wave generator (8) to a direction away from the sound wave generator (8).

6. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The bracket (20) is provided with a cold needle stabilizing device (10) and a slide rail (21), and the cold needle (6) is slidably arranged on the bracket (20) through a telescopic mechanical arm (5) and the slide rail (21); the cold needle (6) is extended through the telescopic mechanical arm (5) and inserted into the cold needle stabilizing device (10), and the temperature of the cold needle (6) is maintained by the low temperature environment inside the cold needle stabilizing device (10).

7. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The sample preparation device further comprises a mechanical arm (9), a conveying device (15) and a telescopic support (16), wherein the telescopic support (16) is arranged below the conveying device (15); the mechanical arm (9) clamps the reagent box (19) and places it on the conveying device (15) via the telescopic support (16).

8. The acoustic levitation microscopy testing device according to claim 7, characterized in that: A reagent kit storage box (17) is provided at the end of the conveying device (15).

9. The acoustic levitation microscopy testing device according to claim 7, characterized in that: The image analysis system comprises a microscope (12) and a display (13); the microscope (12) is arranged on one side of the transmission device (15), and the microscope (12) and the display (13) are connected.

10. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The acoustic levitation microscopy testing device also includes a gas propulsion device, which includes a plurality of jet heads (2). The plurality of jet heads (2) of the gas propulsion device are arranged at the middle and end between the reflector (4) and the sound wave generator (8) to control the balance of the droplets.

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