Detection method and sample analyzer

Abstract

The embodiment of the invention discloses a detection method and a sample analyzer. The method comprises the following steps: controlling a light source device to emit incident light, wherein at least part of the incident light comprises target incident light; acquiring a detection signal of the first target emergent light and acquiring a detection signal of the second target emergent light; determining a detection result of the sample according to the detection signal of the first target emergent light and the detection signal of the second target emergent light; wherein the light intensity value of the target incident light is N times of the light intensity value of the normal incident light, and N is a positive real number greater than 5; the normal incident light is incident light of which the detection signal corresponding to the emergent light after passing through the air is within the measurement range of the measurement device and meets M times of the full voltage range, and M is a positive real number greater than 0.5 and less than 1. By adopting the embodiment of the invention, the accuracy of the measurement result can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sample detection, and in particular to a detection method and a sample analyzer. Background Art

[0002] The optical measurement system is a commonly used measurement system for sample analyzers. Its measurement principle is: a light source (such as a light emitting diode (LED)) emits light of a specific wavelength as the incident light. After the incident light passes through the reaction cup loaded with samples and reagents, the outgoing light is collected by the back-end detector (such as a photodetector (PD)) to collect the light intensity signal. The coagulation reaction result of the sample is obtained by detecting the change in absorbance during the coagulation reaction of the sample in the reaction cup.

[0003] The sample analyzer measurement system based on optical measurement has the problem of inaccurate measurement results due to the high concentration of sample interferents. Therefore, it is urgent to study a detection method to improve the accuracy of the measurement results of the sample analyzer optical system. Summary of the invention

[0004] The embodiments of the present invention provide a detection method and a sample analyzer, which can improve the accuracy of the measurement result of the sample analyzer.

[0005] In a first aspect, an embodiment of the present invention provides a detection method applied to a sample analyzer, wherein the sample analyzer includes a processor, a light source device, and a measuring device; the light source device is used to provide incident light with different light intensity values ​​under the control of the processor; the measuring device is used to detect a detection signal corresponding to the outgoing light after the incident light passes through a reaction cup storing a mixed solution of a sample and a reagent; the detection method includes:

[0006] Control the light source device to emit incident light, wherein at least part of the incident light includes target incident light; obtain a detection signal of a first target emitted light, wherein the first target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution at an early stage of the coagulation reaction; obtain a detection signal of a second target emitted light, wherein the second target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution at a late stage of the coagulation reaction; determine a detection result of the sample according to the detection signal of the first target emitted light and the detection signal of the second target emitted light; wherein the light intensity value of the target incident light is N times the light intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light whose detection signal corresponding to the emitted light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0007] In the embodiment of the present application, the target incident light is used to irradiate the mixed solution, that is, the above-mentioned light source device is adjusted to work in the high light intensity mode. At this time, the signal-to-noise ratio of the measured result is relatively large. In more detail, since the ratio between the light intensity value of the target incident light and the light intensity value of the normal incident light is greater than the ratio between the noise caused by the target incident light and the noise caused by the normal incident light, the ratio between the light intensity value of the above-mentioned target incident light and the light intensity value of the normal incident light is equivalent to the ratio between the change amplitude of the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the coagulation reaction and the change amplitude of the detection signal measured by the measuring device after the normal incident light passes through the mixed solution. Therefore, compared with irradiating the mixed solution with normal incident light, the signal-to-noise ratio obtained by irradiating the mixed solution with the target incident light is larger, thereby improving the repeatability and accuracy of the test results.

[0008] In a possible implementation, the value range of N is [5.5, 6.5].

[0009] In this implementation, the intensity of the target incident light is 5.5 to 6.5 times that of the normal incident light. At this time, the detection signal obtained by directly detecting the target incident light by the measuring device will exceed the measurement range of the measuring device. The above-mentioned target incident light is directly detected by the measuring device, that is, the target incident light is detected by the measuring device after passing through the air. When a high-intensity incident light is used to irradiate the mixed solution, the measured signal-to-noise ratio will be greater, thereby improving the accuracy of the measurement result.

[0010] In a possible implementation, before controlling the light source device to emit incident light, the method further includes: determining the absorbance of the sample solution, and the absorbance is greater than a preset threshold.

[0011] In this implementation, when the absorbance of the sample solution is greater than a preset threshold, that is, when the background of the sample is high, the target incident light is used to irradiate the mixed solution. Due to the high background of the sample, the detection signal of the first target outgoing light will be within the measurement range of the measuring device and greater than the detection signal of the corresponding outgoing light when the normal incident light is used to irradiate the mixed solution. According to the calculation formula of absorbance, it can be concluded that when the difference between the absorbance in the early stage of the coagulation reaction (i.e., the baseline period) and the absorbance in the late stage of the coagulation reaction is the same, the higher the detection signal in the early stage of the coagulation reaction, the greater the change amplitude of the detection signal during the coagulation reaction. Therefore, when the background of the sample is too high, the change amplitude of the detection signal measured by the measuring device during the coagulation reaction of the sample can be increased by controlling the light source device to emit the target incident light. The ratio of the increase in the detection signal is greater than the ratio of the increase in the light source noise due to the increase in the light intensity value, so the signal-to-noise ratio increases. Thereby, the repeatability and accuracy of the test results can be improved.

[0012] In one possible implementation, the sample analyzer has an interference detection channel; determining the absorbance of the sample solution includes: controlling the light source device to emit detection incident light, wherein the detection incident light is normal incident light; obtaining a detection signal of detection output light, wherein the detection output light is output light corresponding to the detection incident light after passing through the sample solution in the interference detection channel; and determining the absorbance of the sample solution according to the detection signal of the detection output light.

[0013] In this implementation, the interference detection channel of the sample analyzer is used to determine the absorbance of the sample solution, and normal incident light is used as the detection incident light. When normal incident light is used to detect the sample background, a more accurate incident light can be measured regardless of whether the sample background is too high, thereby avoiding the light source device also emitting target incident light when the sample background is small, causing the detection signal obtained by the measuring device to exceed the measurement range. When the sample background is high, using target incident light can improve the accuracy of the test results.

[0014] In a possible implementation, the sample analyzer has a first calibration channel, in which a light intensity attenuation plate is provided; the method further includes: obtaining an actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; and calibrating the target incident light according to the actual detection signal.

[0015] In this implementation, since the light intensity value of the target incident light is large, the detection signal of the corresponding outgoing light exceeds the measurement range of the measuring device and cannot be calibrated using the conventional calibration method. Therefore, the embodiment of the present application uses a light intensity attenuation sheet to reduce the light intensity value of the target incident light during calibration, so that the detection signal of the corresponding outgoing light after the target incident light passes through the light intensity attenuation sheet is within the measurement range of the second device. The proportion of the light intensity value reduced by the above-mentioned light intensity attenuation sheet depends on the transmittance of the light intensity attenuation sheet, and the transmittance is the ratio between the light intensity that can pass through the light intensity attenuation sheet and the original light intensity. As an example, the proportion of reducing the light intensity value is inversely proportional to the transmittance of the light intensity attenuation sheet. The lower the transmittance, the greater the proportion of reducing the light intensity value by the light intensity attenuation sheet. As another example, the transmittance of the light intensity attenuation sheet is equal to the ratio of the light intensity that can pass through the first light signal after passing through the light intensity attenuation sheet to the original light intensity of the first light signal.

[0016] In one possible implementation, the target incident light is calibrated according to the actual detection signal, including: if the actual detection signal is greater than the target detection signal, controlling the light source device to reduce the operating current of the light source device; if the actual detection signal is less than the target detection signal, controlling the light source device to increase the operating current of the light source device.

[0017] In this implementation, the target incident light is calibrated according to the actual detection signal, that is, the working current of the light source device is adjusted. The greater the working current, the greater the light intensity value of the target incident light emitted by the light source device. Conversely, the smaller the working current, the smaller the light intensity value of the target incident light emitted by the light source device, until the actual detection signal is equal to the target detection signal.

[0018] In a second aspect, an embodiment of the present invention provides a sample analyzer, comprising: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; a light source device, used to provide incident light with different light intensity values; a measuring device, used to detect a detection signal of an output light generated corresponding to the incident light after passing through the reaction cup containing the mixed solution; and a processor, connected to the light source device and the measuring device, used to execute some or all of the method steps described in the first aspect of this embodiment.

[0019] In a third aspect, an embodiment of the present invention provides a detection method applied to a sample analyzer, the sample analyzer comprising a processor, a light source device and a measuring device; the light source device is used to provide incident light with different light intensity values ​​under the control of the processor; the detection device is used to detect a detection signal of an outgoing light corresponding to the incident light after the incident light passes through a reaction cup storing a mixed solution of a sample and a reagent; the detection method comprises:

[0020] Determine the absorbance of the sample solution; determine the target incident light used to irradiate the mixed solution of the sample and the reagent based on the absorbance, wherein the target incident light is one of the first incident light and the second incident light, and the light intensity value of the first incident light is higher than the light intensity value of the second incident light; control the light source device to emit incident light, wherein the incident light at least partially includes the target incident light; obtain a detection signal of a first emitted light, wherein the first emitted light is the emitted light corresponding to the mixed solution in the early stage of the coagulation reaction after the target incident light irradiates the mixed solution in the late stage of the coagulation reaction; and determine the detection result of the sample solution based on the detection signal of the first emitted light and the detection signal of the second emitted light.

[0021] In the embodiment of the present application, the target incident light is determined according to the absorbance of the sample solution, and the target incident light is one of the first incident light and the second incident light, so that the light intensity value of the incident light irradiating the mixed solution is more flexible, which can improve the accuracy of the measurement result.

[0022] In one possible implementation, the light intensity value of the first incident light is N times the light intensity value of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the output light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0023] In this implementation, the light intensity value of the first incident light is N times the light intensity value of the second incident light, and N is a positive real number greater than 5. At this time, the detection signal of the outgoing light corresponding to the first incident light after passing through the air exceeds the measurement range of the measuring device. When the target incident light is the first incident light, the ratio between the light intensity value of the target incident light and the light intensity value of the second incident light is greater than the ratio between the noise caused by the target incident light and the noise caused by the second incident light. The ratio between the light intensity value of the target incident light and the light intensity value of the second incident light is equal to the ratio between the change amplitude of the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the coagulation reaction and the change amplitude of the detection signal measured by the measuring device after the second incident light passes through the mixed solution. Therefore, the signal-to-noise ratio is increased, thereby improving the repeatability and accuracy of the test results.

[0024] In a possible implementation, determining the target incident light for irradiating the mixed solution of the sample and the reagent based on the absorbance includes: if the absorbance is greater than a preset threshold, determining the target incident light as the first incident light; if the absorbance is less than the preset threshold, determining the target incident light as the second incident light.

[0025] In this implementation, when the sample absorbance is greater than a preset threshold, the target incident light is determined to be the first incident light, so the detection signal measured by the measuring device in the early stage of the coagulation reaction is larger. According to the calculation formula of absorbance, it can be concluded that when the difference between the absorbance in the early stage of the coagulation reaction and the absorbance in the late stage of the coagulation reaction is the same, the higher the detection signal in the early stage of the coagulation reaction, the greater the change amplitude of the detection signal during the coagulation reaction. Therefore, when the background of the sample is too high, determining the target incident light as the first incident light can increase the change amplitude of the detection signal measured by the measuring device during the coagulation reaction of the sample. The ratio of the increase in the detection signal is greater than the ratio of the increase in light source noise due to the increase in light intensity, so the signal-to-noise ratio increases. Thereby, the accuracy of the test results can be improved.

[0026] In one possible implementation, the sample analyzer has a first calibration channel, in which a light intensity attenuation plate is provided; the method further includes: when the target incident light is determined to be the first incident light, obtaining an actual detection signal of the output light corresponding to the target incident light after passing through the first calibration channel; and calibrating the target incident light according to the actual detection signal.

[0027] For the description of this implementation method, please refer to the description of the first aspect above, which will not be described in detail here.

[0028] In one possible implementation, the sample analyzer has a second calibration channel; the method further includes: when the target incident light is determined to be the second incident light, obtaining an actual detection signal of the output light corresponding to the target incident light after passing through the second calibration channel; and calibrating the target incident light according to the actual detection signal.

[0029] In this implementation, the above-mentioned second calibration channel is a calibration channel for the second incident light. When the second incident light is used as the target incident light, since the detection signal of the output light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device, the second incident light can be calibrated directly according to the actual detection signal.

[0030] In one possible implementation, the sample analyzer has an interference detection channel; determining the absorbance of the sample solution includes: controlling the light source device to emit detection incident light, wherein the detection incident light is normal incident light; obtaining a detection signal of detection output light, wherein the detection output light is output light corresponding to the detection incident light after passing through the sample solution in the interference detection channel; and determining the absorbance of the sample solution according to the detection signal of the detection output light.

[0031] For the description of this implementation method, please refer to the description of the first aspect above, which will not be described in detail here.

[0032] In one possible implementation, the detected incident light is normal incident light, wherein the normal incident light is the detection signal of the corresponding outgoing light after passing through the air, which is within the measurement range of the measuring device and satisfies M times the full voltage range of the incident light, wherein M is a positive real number greater than 0.5 and less than 1.

[0033] In one possible implementation, the sample analyzer includes multiple detection channels; the light source device is used to switch different wavelengths of the incident light during the detection process under the control of the processor; the method also includes: determining the time segment of the target incident light in the incident light according to the detection items of the detection channel and the target incident light; and determining the detection signals of the first outgoing light and the second outgoing light according to the time segment.

[0034] In this implementation, the sample analyzer includes multiple detection channels and can perform multiple detection items simultaneously. During the detection process, the light source device can switch different wavelengths of incident light, that is, the incident light emitted by the light source device is a series of lights with different wavelengths and different light intensity values. This series of lights occupies a time segment respectively, which contains the target incident light.

[0035] In a possible implementation, after determining that the target incident light is the second incident light and acquiring the detection signal of the first outgoing light and acquiring the detection signal of the second outgoing light, the method further includes: amplifying the detection signal of the first outgoing light and the detection signal of the second outgoing light.

[0036] In this implementation, when the target incident light is the second incident light, the measuring device can be controlled to amplify the detection signal of the first outgoing light and the detection signal of the second outgoing light, which can also increase the signal-to-noise ratio, thereby improving the repeatability and accuracy of the test results.

[0037] In a fourth aspect, an embodiment of the present invention provides a sample analyzer, comprising: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; a light source device, used to provide incident light with different light intensity values; a measuring device, used to detect a detection signal of an output light generated corresponding to the incident light after passing through the reaction cup containing the mixed solution; and a processor, connected to the light source device and the measuring device, for executing some or all of the method steps described in the third aspect of this embodiment.

[0038] In a fifth aspect, an embodiment of the present application provides a computer-readable storage medium, which stores instructions. When the computer-readable storage medium is run on a computer, some or all of the method steps described in the first aspect or the third aspect of the embodiment are executed. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the accompanying drawings required for use in the embodiment of the present invention will be described below.

[0040] Figure 1 is a structural schematic diagram of an optical measurement system provided in an embodiment of the present application;

[0041] Figure 2 is a curve diagram of the absorbance of a common interfering substance provided in the examples of the present application;

[0042] Figure 3 is a coagulation reaction curve provided in an embodiment of the present application;

[0043] Figure 4 It is another coagulation reaction curve provided in the embodiment of the present application;

[0044] Figure 5 It is a flow chart of a detection method provided in an embodiment of the present application;

[0045] Figure 6 It is a flow chart of another detection method provided in an embodiment of the present application;

[0046] Figure 7 It is a flow chart of another detection method provided in an embodiment of the present application;

[0047] Figure 8 It is a structural schematic diagram of a sample analyzer provided in an embodiment of the present application. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0049] The terms "first", "second", etc. in the specification and claims of this application and the above-mentioned drawings are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0050] Reference to "embodiments" in this application means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described in this application may be combined with other embodiments.

[0051] The technical solution provided in the embodiment of the present application can be applied to an optical measurement system, which is a commonly used measurement system for a sample analyzer, which may include a coagulation analyzer. The measurement principle is: a light source (such as an LED, etc.) emits light of a specific wavelength as incident light. After the incident light passes through a reaction container containing a sample or a reagent, the outgoing light is collected by a rear-end measurement device for a light intensity signal. The coagulation reaction result of the sample is obtained by detecting the change in absorbance during the coagulation reaction of the sample in the reaction container.

[0052] See also Figure 1 , Figure 1 Schematic diagram of the structure of an optical measurement system provided in an embodiment of the present application. Figure 1 As shown, the optical measurement system includes a light source device 101, a reaction cup 102 and a measuring device 103, and the measuring device 103 is a device for converting an optical signal into an electrical signal. Exemplarily, the measuring device 103 can be a photodiode (PD). The light source device 101 adjusts the working current to emit optical signals of different light intensities. The light source device 101 can be an LED, and examples are not given here one by one. The reaction cup 102 is filled with samples with different absorbances, and the reaction cup is a light-transmitting container, such as a beaker or a reaction cup. The measuring device 103 is used to collect optical signals, and convert the optical signals into analog electrical signals using a photoelectric sensor, and then convert the analog electrical signals into digital electrical signals. During the coagulation reaction, the amplitude of the change of the digital electrical signal is a useful signal obtained by measurement. The light source device 101 can emit optical signals of different intensities. Figure 1 The light signal emitted by the central light source is the incident light 104 , and the light signal after the incident light 104 passes through the reaction cup is the outgoing light 105 .

[0053] like Figure 1 As shown, the incident light 104 is emitted from the light source, and is measured by the measuring device 103 after passing through the reaction cup 102. During the coagulation reaction of the sample in the reaction cup, the absorbance of the sample changes (for example, the absorbance gradually increases during the coagulation reaction), so the light intensity of the outgoing light 105 gradually decreases. The measuring device 103 will record the voltage value change amplitude obtained by measuring the outgoing light 105 during the reaction process, which is equivalent to the light intensity change amplitude of the light signal after passing through the reaction cup. It should be noted that during the reaction process of the sample, the intensity of the incident light 104 emitted by the light source device 101 is a constant value.

[0054] See also Figure 2 , Figure 2 is a graph showing the absorbance of a common interfering substance provided in the examples of this application. Figure 2 As shown in the figure, common interferents such as hemolysis, icterus and lipemia (collectively referred to as HIL interferents) have different degrees of absorption peaks at the main measurement wavelengths, such as 405nm, 575nm, 660nm, and 800nm, in the optical measurement device of the coagulation analyzer. Coagulation analyzers based on optical measurement methods are prone to deviations in test results due to the presence of interferents in plasma samples (here refers to deviations in measurement, not including pathological deviations). Figure 2 The horizontal axis is the wavelength of the light signal, and the vertical axis is the absorbance.

[0055] See also Figure 3 , Figure 3 This is a coagulation reaction curve provided in the embodiment of the present application. Figure 3 As shown, the curve is the test result of a normal plasma sample. The ordinate is the voltage value of the sample obtained by the measuring device during the coagulation reaction. Optionally, the ordinate is the digital value (Analog-to-digital, AD) output by the measuring device after converting the optical signal into an analog electrical signal and then passing through an analog-to-digital converter. Figure 3 The change range of the middle curve is the change range of the voltage value during the coagulation reaction, and the horizontal axis is the coagulation reaction time of the sample.

[0056] like Figure 3 As shown, the duration of the coagulation reaction starts from the time when the last reagent is added to the sample solution and the mixed solution is put back into the measurement channel. The voltage value corresponding to the highest point of the curve is the voltage value measured by the measuring device when the incident light passes directly through the air, and when the mixed solution is put back into the measurement channel, the voltage value will drop sharply. Subsequently, the curve enters the first plateau period (i.e., the baseline period or the early stage of the coagulation reaction). The voltage value obtained by the measuring device when measuring the light signal passing through the sample during the baseline period is higher because the background of normal plasma samples is smaller, that is, the absorbance of normal plasma samples is smaller in the early stage of the coagulation reaction. At this time, the light intensity value of the light signal after passing through the sample is higher. Then, during the coagulation reaction, due to the gradual increase in the absorbance of the sample, the voltage value obtained by the measuring device when measuring the light signal after passing through the sample gradually decreases, as shown in FIG. Figure 3 , the curve drops gently during the coagulation reaction. Finally, in the second plateau of the curve (i.e., the late stage of the coagulation reaction), the absorbance of the sample reaches a maximum value and tends to be stable (in some cases, it may also rise slowly or fluctuate), so the voltage value obtained by the measuring device measuring the light signal after passing through the sample reaches a minimum value and tends to be stable (in some cases, it may also drop slowly).

[0057] See also Figure 4 , Figure 4 This is another coagulation reaction curve provided in the embodiment of the present application. Figure 4 As shown, this curve is the test result of lipemia sample. Figure 4 The horizontal and vertical coordinates refer to the above Figure 3 The description of the horizontal and vertical coordinates of is not detailed here. Figure 4 As shown in the figure, the voltage value of the coagulation reaction curve of the lipemia sample changes slightly during the coagulation reaction, which is much smaller than Figure 3The voltage value variation range of normal plasma samples during the coagulation reaction. The reasons are as follows: compared with normal plasma samples, the concentration of interfering substances in lipemia samples is greater, that is, the absorbance of interfering substances (i.e. background) in lipemia samples is higher. For samples with a high background, since the sample absorbance is higher in the early stage of the coagulation reaction, the voltage value measured by the measuring device in the early stage of the coagulation reaction will be lower. Because the absorbance variation range of the sample is small during the entire coagulation reaction, the voltage value variation range of the sample measured by the measuring device during the coagulation reaction is small. In other words, the useful signal that affects the measurement result in the entire measurement process is small, and the signal-to-noise ratio is low, resulting in poor repeatability and accuracy of the measurement result. For Figure 4 The details of the coagulation reaction curve can be found in the above Figure 3 The description will not be described in detail here.

[0058] Based on the above problems, the embodiment of the present application provides a measurement method, which can solve the problem of inaccurate measurement results. The method provided by the embodiment of the present application is specifically described below.

[0059] See also Figure 5 , Figure 5 is a flow chart of a detection method provided in an embodiment of the present application. For the description of the light source device and the measuring device involved in the embodiment of the present application, please refer to the above description of Figure 1 The following is a detailed description of the steps involved in the above process.

[0060] 501. Control the light source device to emit incident light, wherein at least a portion of the incident light includes target incident light.

[0061] The intensity value of the target incident light is N times the intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light that has a detection signal of the corresponding outgoing light after passing through the air within the measurement range of the measurement device and satisfies the full voltage range M times, where M is a positive real number greater than 0.5 and less than 1. Optionally, the value range of N is [5.5,6.5].

[0062] Exemplarily, the measuring device may use a 12-bit, 16-bit or 24-bit digital-to-analog conversion chip; in some examples, their measurement range may be [-2.5V, +2.5V]. When the maximum value of the normal light intensity value is directly measured, it can reach 70%-80% of the full voltage of the measuring device, preferably 75% of the full voltage of the chip. The light intensity value of the target incident light (i.e., the high light intensity value) can be between 5.7 and 6.3 times the normal light intensity value (maximum value), preferably 5.9 times. In the embodiment of the present application, the detection signal obtained by directly detecting the target incident light by the measuring device will exceed the measurement range of the measuring device. When a high-intensity incident light is used to irradiate the mixed solution, the measured signal-to-noise ratio will be greater, thereby improving the accuracy of the measurement result.

[0063] Exemplarily, the incident light emitted by the light source device is a series of lights of different wavelengths and different light intensities. For example, the wavelength of the first beam of incident light is 405nm, and the light intensity value is 26000, the wavelength of the second beam of incident light is 405nm, and the light intensity value is 154000, the wavelength of the third beam of incident light is 660nm, and the light intensity value is 26000, the wavelength of the fourth beam of incident light is 660nm, and the light intensity value is 154000, etc., and examples are not given one by one here. The above-mentioned target incident light can be a part of a series of lights emitted by the light source device. Correspondingly, at the measuring device end, the measuring device detects the target incident light after passing through the mixed solution and the corresponding target outgoing light obtains a detection signal, and the processor determines the detection result according to the detection signal.

[0064] Optionally, before controlling the light source device to emit incident light, the absorbance of the sample solution is first determined, and the absorbance is greater than a preset threshold. In the embodiment of the present application, when the absorbance of the sample solution is greater than the preset threshold, the target incident light is used to irradiate the mixed solution. Due to the high background of the sample, the detection signal of the outgoing light corresponding to the target incident light after passing through the mixed solution in the early stage of the coagulation reaction will be within the measurement range of the measuring device, and will be greater than the detection signal of the corresponding outgoing light when the mixed solution is irradiated with normal incident light. According to the calculation formula of absorbance, it can be concluded that when the difference between the absorbance in the early stage of the coagulation reaction (i.e., the baseline period) and the difference between the absorbance in the late stage of the coagulation reaction are the same, the higher the detection signal in the early stage of the coagulation reaction, the greater the change amplitude of the detection signal during the coagulation reaction. Therefore, when the background of the sample is too high, the change amplitude of the detection signal measured by the measuring device during the coagulation reaction of the sample can be increased by controlling the light source device to emit the target incident light. Furthermore, since the ratio of the increase in the detection signal is greater than the ratio of the increase in light source noise due to the increase in light intensity, the signal-to-noise ratio is increased, thereby improving the repeatability and accuracy of the test results.

[0065] Optionally, when the target incident light is normal incident light and can be accurately measured (ie, the measured signal-to-noise ratio is large, greater than 3.0), the preset threshold is the maximum value of the sample absorbance.

[0066] Exemplarily, the absorbance (A) is calculated as follows:

[0067]

[0068] The absorbance calculated by this formula is a dimensionless quantity, where T 入射光 is the intensity of the incident light, T 出射光 is the intensity of the outgoing light after passing through the sample solution.

[0069] Optionally, the sample analyzer has an interference detection channel, and the processor controls the light source device to emit a detection incident light, which is a normal incident light, and then obtains a detection signal of the detection output light, and determines the absorbance of the sample solution based on the detection signal of the detection output light. In an embodiment of the present application, the interference detection channel of the above-mentioned sample analyzer is used to determine the absorbance of the sample solution, and normal incident light is used as the detection incident light. When normal incident light is used to detect the sample background, a more accurate incident light can be measured regardless of whether the sample background is too high, thereby avoiding the light source device also emitting a target incident light to detect the sample background when the sample background is small, causing the detection signal obtained by the measuring device to measure the detection output light to exceed the measurement range.

[0070] Optionally, the sample analyzer has a first calibration channel, in which a light intensity attenuation plate is provided, and the target incident light is calibrated according to an actual detection signal of the corresponding outgoing light after the target incident light passes through the first calibration channel. In the embodiment of the present application, the light intensity attenuation plate is used during calibration to reduce the light intensity value of the target incident light, so that the detection signal of the corresponding outgoing light after the target incident light passes through the light intensity attenuation plate is within the measurement range of the measuring device.

[0071] Exemplarily, there are 24 channels in the sample analyzer mentioned in the embodiment of the present application, one is a calibration channel for normal light intensity, one is a calibration channel for high light intensity, one is a detection channel for interference, and the remaining 21 are ordinary detection channels, and the above-mentioned first calibration channel belongs to the calibration channel for high light intensity. Light intensity calibration and detection of mixed solution can be carried out simultaneously. For example, during the detection process, the incident light of high light intensity or the incident light of normal light intensity used in the ordinary detection channel is calibrated once every 100ms. In some examples, in 21 ordinary detection channels, it can be used to carry out a variety of detection projects, such as coagulation method, colorimetric turbidimetry, chromogenic substrate method, etc. Among them, the change degree sensitive wavelength of the coagulation method is 660nm and 450nm, the change degree sensitive wavelength of the colorimetric turbidimetry is 575nm, and the change degree sensitive wavelength of the chromogenic substrate method is 405nm.

[0072] Optionally, if the actual detection signal is greater than the target detection signal, the light source device is controlled to reduce the working current of the light source device; if the actual detection signal is less than the target detection signal, the light source device is controlled to increase the working current of the light source device. In an embodiment of the present application, the target incident light is calibrated according to the actual detection signal, that is, the working current of the light source device is adjusted. The greater the working current, the greater the light intensity value of the target incident light emitted by the light source device. Conversely, the smaller the working current, the smaller the light intensity value of the target incident light emitted by the light source device, until the actual detection signal is equal to or closest to the target detection signal. This makes the light intensity value of the target incident light more accurate, thereby improving the accuracy of the measurement result.

[0073] 502. Acquire a detection signal of the first target emitted light.

[0074] The first target emitted light referred to herein is the emitted light corresponding to the target incident light after passing through the mixed solution in the early stage of the coagulation reaction.

[0075] In the embodiment of the present application, the early stage of the coagulation reaction (i.e., the baseline stage) is the part of the early stage of the coagulation reaction curve where the AD value changes relatively slowly. In some embodiments, it is possible to judge whether the AD value changes slowly based on the amplitude of the change in the AD value, such as by using methods such as difference, slope, and integration to judge the part where the AD value changes slowly at the beginning of the coagulation reaction, and then the section where the part is located can be determined as the early stage of the coagulation reaction. In some embodiments, the detection signals (AD values) of multiple time points in a partial window (intervals where the AD value changes relatively steadily) in the early stage of the coagulation reaction can be collected, and the average value of the multiple AD values ​​can be used as the detection signal of the first target emitted light.

[0076] 503. Acquire a detection signal of the second target output light.

[0077] The second target emitted light referred to herein is the emitted light corresponding to the target incident light after passing through the mixed solution in the late stage of the coagulation reaction.

[0078] In an embodiment of the present application, the late stage of the coagulation reaction is the part of the late stage of the coagulation reaction curve where the AD value changes relatively slowly. In some embodiments, it is also possible to judge whether the AD value changes slowly based on the amplitude of the change in the AD value. For example, methods such as difference, slope or integration can be used to judge the part where the AD value changes relatively slowly in the late stage of the coagulation reaction. In some embodiments, after determining the late stage of the coagulation reaction, all or part of the AD values ​​in this interval can be collected for calculation to obtain a detection signal of the second target output light, such as an average value. Exemplarily, the late stage of the coagulation reaction is divided into three windows, and the AD values ​​corresponding to three time points are randomly selected in each window, and then the average value of these nine AD values ​​is calculated as the detection signal of the second target output light.

[0079] 504. Determine a detection result of the sample according to the detection signal of the first target output light and the detection signal of the second target output light.

[0080] In some embodiments, the time point at which the coagulation reaction ends can be obtained based on the detection signal of the first target output light and the detection signal of the second target output light. Exemplarily, the time point corresponding to the middle value of the detection signal obtained in the early stage of the coagulation reaction and the detection signal obtained in the late stage of the coagulation reaction is selected as the time point at which the coagulation reaction ends, so as to obtain a coagulation reaction time from the time point at which the coagulation reaction starts (for example, the time point at which the last reagent is put into the mixed solution and then put back into the detection channel) to the time point at which the above coagulation reaction ends, and then the detection result of the sample can be determined. Optionally, the concentration of the sample can be further obtained based on the above coagulation reaction time. The above coagulation reaction time and the time point at which the coagulation reaction ends can be found in Figure 3 .

[0081] In the embodiment of the present application, the target incident light is used to irradiate the mixed solution, that is, the above-mentioned light source device is adjusted to work in the high light intensity mode. At this time, the signal-to-noise ratio of the measured result is relatively large. In more detail, the ratio of the light intensity value of the target incident light to the light intensity value of the normal incident light is greater than the ratio of the increase in noise caused by the increase in the light intensity value. The ratio of the light intensity value of the above-mentioned target incident light to the light intensity value of the normal incident light is equal to the ratio of the change amplitude of the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the coagulation reaction to the change amplitude of the detection signal measured by the measuring device after the normal incident light passes through the mixed solution. Therefore, compared with irradiating the mixed solution with normal incident light, the signal-to-noise ratio obtained by irradiating the mixed solution with the target incident light is larger, thereby improving the repeatability and accuracy of the test results.

[0082] See also Figure 6 , Figure 6 is a flow chart of another detection method provided in the embodiment of the present application. The description of the light source device and the measuring device involved in the embodiment of the present application can refer to the above description of Figure 1 The following is a detailed description of the steps involved in the above process.

[0083] 601. Determine the absorbance of the sample solution.

[0084] In some embodiments, the sample analyzer has an interference detection channel, and the processor controls the light source device to emit detection incident light, and the detection incident light is normal incident light. Then, in the interference detection channel, the corresponding outgoing light (also referred to as "detected outgoing light") is obtained after the detection incident light passes through the sample solution. The absorbance of the sample solution is determined based on the detection signal of the detected outgoing light.

[0085] Optionally, the detected incident light is normal incident light, and the maximum value of the normal incident light is that the detection signal of the corresponding outgoing light after passing through the air is within the measurement range of the measuring device, and the detection signal of the corresponding outgoing light after the normal incident light passes through the air satisfies M times the full voltage range of the incident light, where M is a positive real number greater than 0.5 and less than 1, preferably 0.75.

[0086] Exemplarily, the detection incident light may be the normal incident light of the first part of the series of lights emitted by the light source device, for example, the wavelength of the first incident light is 405nm, the light intensity is 26000, the wavelength of the second incident light is 405nm, the light intensity is 154000, the wavelength of the third incident light is 660nm, the light intensity is 26000, the wavelength of the fourth incident light is 660nm, the light intensity is 154000, etc., then the detection incident light may be the first incident light. After the light source device emits this series of lights, the absorbance of the sample can be detected immediately, so that after the test is completed, the target outgoing light can be determined in the outgoing light corresponding to the series of lights.

[0087] 602. Determine target incident light for irradiating a mixed solution of the sample and the reagent according to the absorbance.

[0088] The target incident light is a beam of the above series of lights emitted by the light source device, and the series of lights includes the first incident light, the second incident light, etc. The target incident light is determined in the series of lights according to the absorbance of the sample, and the light intensity value of the above first incident light is higher than the light intensity value of the second incident light. Exemplarily, the light intensity values ​​and wavelengths of the above first incident light and the second incident light can be different, and can be used for different detection items and detection items using different methods, such as coagulation method, colorimetric turbidimetric method, chromogenic substrate method, etc., wherein the change degree sensitive wavelengths of the coagulation method are 660nm and 450nm, the change degree sensitive wavelength of the colorimetric turbidimetric method is 575nm, and the change degree sensitive wavelength of the chromogenic substrate method is 405nm.

[0089] Optionally, the light intensity value of the first incident light is N times the light intensity value of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the output light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0090] Optionally, if the absorbance is greater than a preset threshold, the target incident light is determined to be the first incident light; if the absorbance is less than the preset threshold, the target incident light is determined to be the second incident light.

[0091] Optionally, the sample analyzer has a first calibration channel, in which a light intensity attenuation plate is provided. When the target incident light is determined to be the first incident light, the processor obtains an actual detection signal of the output light corresponding to the target incident light after passing through the first calibration channel, and then calibrates the target incident light according to the actual detection signal.

[0092] Optionally, the sample analyzer has a second calibration channel. When it is determined that the target incident light is the second incident light, the processor obtains an actual detection signal of the output light corresponding to the target incident light after passing through the second calibration channel, and then calibrates the target incident light according to the actual detection signal.

[0093] For a detailed description of this step, please refer to the above Figure 5 The description of step 501 will not be described in detail here.

[0094] 603. Control the light source device to emit incident light.

[0095] The incident light at least partially includes target incident light.

[0096] Optionally, the sample analyzer includes a plurality of detection channels, and the light source device is used to switch different wavelengths of incident light during the detection process under the control of the processor, and determine the time segment of the target incident light in the incident light according to the detection items of the detection channel and the target incident light. Because the above-mentioned incident light is a series of lights with different light intensity values ​​and wavelengths, a part of which is the target incident light, the target incident light will occupy a time segment of the incident light.

[0097] 604. Obtain a detection signal of the first emitted light.

[0098] Optionally, the detection signals of the first emergent light and the second emergent light are determined according to a time segment.

[0099] For a detailed description of this step, please refer to the above Figure 5 The description of step 502 will not be described in detail here.

[0100] 605. Obtain a detection signal of the second emitted light.

[0101] Optionally, the detection signals of the first emergent light and the second emergent light are determined according to a time segment.

[0102] For a detailed description of this step, please refer to the above Figure 5 The description of step 503 is not described in detail here.

[0103] 606. Determine a detection result of the sample solution according to the detection signal of the first emergent light and the detection signal of the second emergent light. Optionally, when the sample absorbance is greater than a threshold and the target incident light is the second incident light, after acquiring the detection signal of the first emergent light and acquiring the detection signal of the second emergent light, the detection signal of the first emergent light and the detection signal of the second emergent light are amplified (i.e., high gain mode), and the signal-to-noise ratio obtained at this time is relatively large.

[0104] Optionally, when the sample absorbance is less than a threshold value and the target incident light is the second incident light, a measurement result is directly obtained after obtaining a detection signal of the first outgoing light and a detection signal of the second outgoing light (ie, a normal light intensity mode).

[0105] As an example, the sample background is 2, and the absorbance variation of the sample coagulation reaction is 0.02. At this time, the normal light intensity mode, high gain mode and high light intensity mode are used for measurement. The measurement data are as follows:

[0106]

[0107] In the above table, the initial AD is the AD obtained by the measuring device directly measuring the light signal emitted by the light source device. The AD in the early stage of the reaction is the AD obtained by the measuring device measuring the light signal passing through the mixed solution in the early stage of the coagulation reaction of the mixed solution. The AD after the reaction is the AD obtained by the measuring device measuring the light signal passing through the mixed solution in the late stage of the coagulation reaction of the mixed solution. The AD change amplitude is the difference between the AD in the early stage of the reaction and the AD in the late stage of the reaction. The AD change amplitude is a useful signal of the measurement result.

[0108] As can be seen from the chart, since the background of the mixed solution is 2, which is greater than the preset threshold value of 1.5, that is, the concentration of the interfering substance in the mixed solution is relatively large, when the light source device works in the high light intensity mode, the signal-to-noise ratio after signal processing is 6.93, which is greater than the signal-to-noise ratio after signal processing when the light source device works in the normal light intensity mode (3.90) and the signal-to-noise ratio after signal processing when the measuring device works in the high gain mode (5.57). In more detail, the ratio of the AD change amplitude in the high light intensity mode and the normal light intensity mode (69.3 / 11.7) is equal to the ratio of the initial AD (154000 / 26000), equal to the ratio of the AD in the early stage of the coagulation reaction (1540 / 260), and greater than the ratio of the noise after signal processing (10 / 3). In other words, during the coagulation reaction, the ratio of the intensity of the light signal increased by the light source device is equal to the ratio of the increase in the AD change amplitude caused by the increase in the light intensity value, and is greater than the ratio of the increase in the light source noise caused by the increase in the light source signal intensity. It can be seen that when the background of the mixed solution is high, the method of adjusting the light source device to work in the high light intensity mode can effectively improve the signal-to-noise ratio of the measurement, thereby solving the problem of deviation in the measurement results caused by the presence of interferents in the mixed solution, and further improving the accuracy of the measurement results.

[0109] It can be seen that both high gain and high light intensity modes can improve the signal-to-noise ratio, among which the high light intensity mode is more effective in improving the signal-to-noise ratio. For mixed solutions with high concentrations of interfering substances, the high light intensity mode is preferred. Considering that the signal of a normal mixed solution (or a mixed solution with a background less than a preset threshold) is outside the acquisition range of the measurement board in the high light intensity mode, the normal mixed solution cannot use the high light intensity mode, but is suitable for the high gain mode.

[0110] In the embodiment of the present application, the target incident light is determined according to the absorbance of the sample solution, and the target incident light is one of the first incident light and the second incident light, so that the light intensity value of the incident light irradiating the mixed solution is more flexible, which can improve the accuracy of the measurement result.

[0111] See also Figure 7 , Figure 7 is a flow chart of another detection method provided in the embodiment of the present application. The description of the light source device and the measuring device involved in the embodiment of the present application can refer to the above description of Figure 1 The following is a detailed description of the steps involved in the above process.

[0112] 701. Determine the sample absorbance.

[0113] For a detailed description of this step, please refer to the above Figure 5 The description of step 501 will not be described in detail here.

[0114] 702. Determine whether the absorbance is greater than a preset threshold. If the absorbance is greater than the preset threshold, execute step 703; if the absorbance is less than the preset threshold, execute step 704.

[0115] 703. Control the light source device to operate in a high light intensity mode.

[0116] For a detailed description of this step, please refer to the above Figure 5 The description of steps 501, 502, and 503 will not be described in detail here.

[0117] 704. Control the light source device to operate in a normal light intensity mode.

[0118] For a detailed description of this step, please refer to the above Figure 6 The description of steps 603, 604, and 605 will not be described in detail here.

[0119] 705. Control the measuring device to operate in a high gain mode.

[0120] For a detailed description of the high gain mode, refer to the above Figure 6 The description of step 606 will not be described in detail here.

[0121] Optionally, when the sample absorbance is less than a threshold and the target incident light is the second incident light, the measurement result is directly obtained after obtaining the detection signal of the first outgoing light and the detection signal of the second outgoing light (ie, normal light intensity mode), and the measuring device does not need to be controlled to operate in high gain mode.

[0122] The following is an introduction to the device provided in the embodiments of the present application.

[0123] The present application divides the functional modules of the device according to the above method embodiments. For example, each functional module can be divided according to each function, or two or more functions can be integrated into one processing module. The above integrated modules can be implemented in the form of hardware or software functional modules. It should be noted that the division of modules in the present application is schematic and is only a logical function division. There may be other division methods in actual implementation. Figure 8 The device of the embodiment of the present application is described in detail.

[0124] See also Figure 8 , Figure 8It is a structural schematic diagram of a sample analyzer provided in an embodiment of the present application. The sample analyzer includes a processor 801, a light source device 802, a measuring device 103 and a detection channel 804. The processor 801 is connected to the light source device 802 and the measuring device 103, such as an electrical connection, a communication connection, etc., and is used to control the light source device 802 and the measuring device 103 to obtain a detection result. The light source device 802 is used to at least provide incident light with different light intensities, and the measuring device 103 is used to detect the detection signal of the corresponding outgoing light generated after the incident light passes through the reaction cup of the mixed solution. The detection channel 804 is at least partially used to load the reaction cup with a mixed solution of a sample and a reagent. At least one detection channel is required in the sample analyzer. It should be noted that Figure 8 The processor, light source device, measuring device and detection channel shown in the figure are only examples. For the specific functions or execution steps of the processor, light source device and measuring device, etc., reference may be made to the above method embodiments and will not be described in detail here.

[0125] An embodiment of the present application also provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program for electronic data exchange, and the computer program enables a computer to execute part or all of the steps of any method for implementing a key function as recorded in the above method embodiments.

[0126] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the described order of actions, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present application.

[0127] A person of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing related hardware through a program, and the program can be stored in a computer-readable memory, which can include: a flash drive, a read-only memory, a random access memory, a magnetic disk or an optical disk, etc.

[0128] The embodiments of the present application are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation method and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A detection method applied to a sample analyzer, characterized in that: The sample analyzer includes a processor, a light source device and a measuring device; the light source device is used to provide incident light with different light intensity values ​​under the control of the processor; the measuring device is used to detect the detection signal of the incident light corresponding to the outgoing light after the incident light passes through the reaction cup storing the mixed solution of the sample and the reagent; the detection method includes: Controlling the light source device to emit incident light, wherein at least a portion of the incident light includes target incident light; Acquire a detection signal of a first target emitted light, wherein the first target emitted light is emitted light corresponding to the target incident light after passing through the mixed solution at the early stage of the coagulation reaction; Acquire a detection signal of a second target emitted light, wherein the second target emitted light is emitted light corresponding to the target incident light after passing through the mixed solution at the late stage of the coagulation reaction; Determining a detection result of the sample according to a detection signal of the first target output light and a detection signal of the second target output light; Among them, the light intensity value of the target incident light is N times the light intensity value of the normal incident light, and N is a positive real number greater than 5; the normal incident light is the incident light that is M times the full voltage range and the detection signal of the corresponding outgoing light after passing through the air is within the measurement range of the measuring device and meets the full voltage range, and M is a positive real number greater than 0.5 and less than 1.

2. The method according to claim 1, characterized in that The value range of N is [5.5, 6.5].

3. The method according to claim 1, characterized in that Before controlling the light source device to emit incident light, the method further includes: The absorbance of the sample solution is determined, and the absorbance is greater than a preset threshold.

4. The method according to claim 3, characterized in that The sample analyzer has an interference detection channel; Determining the absorbance of the sample solution comprises: Controlling the light source device to emit detection incident light, wherein the detection incident light is normal incident light; Acquiring a detection signal of the detected emitted light, wherein the detected emitted light is the emitted light corresponding to the light after passing through the sample solution in the interference detection channel; The absorbance of the sample solution is determined according to the detection signal of the detected emitted light.

5. The method according to any one of claims 1 to 4, characterized in that: The sample analyzer has a first calibration channel, and a light intensity attenuation plate is arranged in the first calibration channel; the method further includes: Acquire an actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; The target incident light is calibrated according to the actual detection signal.

6. The method according to claim 5, characterized in that The step of calibrating the target incident light according to the actual detection signal comprises: If the actual detection signal is greater than the target detection signal, controlling the light source device to reduce the operating current of the light source device; If the actual detection signal is smaller than the target detection signal, the light source device is controlled to increase the operating current of the light source device.

7. A sample analyzer, characterized in that: include: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; A light source device, used for providing incident light with different light intensity values; A measuring device, used for detecting a detection signal of an outgoing light generated corresponding to the incident light passing through the reaction cup of the mixed solution; A processor is connected to the light source device and the measuring device, and is used to execute the method according to any one of claims 1 to 6.

8. A detection method applied to a sample analyzer, characterized in that: The sample analyzer comprises a processor, a light source device and a measuring device; the light source device is used to provide incident light of different light intensity values ​​under the control of the processor; The detection device is used to detect a detection signal of the incident light corresponding to the outgoing light after the incident light passes through a reaction cup storing a mixed solution of a sample and a reagent; the detection method comprises: Determine the absorbance of the sample solution; Determining, according to the absorbance, a target incident light for irradiating a mixed solution of the sample and the reagent, wherein the target incident light is one of a first incident light and a second incident light, and a light intensity value of the first incident light is higher than a light intensity value of the second incident light; Controlling the light source device to emit incident light, wherein the incident light at least partially includes the target incident light; Acquiring a detection signal of a first emitted light, where the first emitted light is emitted light corresponding to the mixed solution in the early stage of a coagulation reaction after the target incident light irradiates the mixed solution; Acquiring a detection signal of a second emitted light, where the second emitted light is emitted light corresponding to the mixed solution in the late stage of the coagulation reaction after the target incident light irradiates the mixed solution; A detection result of the sample solution is determined according to the detection signal of the first emitted light and the detection signal of the second emitted light.

9. The method according to claim 8, characterized in that The light intensity value of the first incident light is N times the light intensity value of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the outgoing light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

10. The method according to claim 8, characterized in that The step of determining, based on the absorbance, a target incident light for irradiating a mixed solution of the sample and the reagent comprises: If the absorbance is greater than a preset threshold, determining that the target incident light is the first incident light; If the absorbance is less than a preset threshold, the target incident light is determined to be the second incident light.

11. The method according to claim 8, characterized in that The sample analyzer has a first calibration channel, and a light intensity attenuation plate is arranged in the first calibration channel; the method further includes: When it is determined that the target incident light is the first incident light, obtaining an actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; The target incident light is calibrated according to the actual detection signal.

12. The method according to claim 8, characterized in that The sample analyzer has a second calibration channel; the method further comprises: When it is determined that the target incident light is the second incident light, obtaining an actual detection signal of the outgoing light corresponding to the target incident light after passing through the second calibration channel; The target incident light is calibrated according to the actual detection signal.

13. The method according to claim 8, characterized in that The sample analyzer has an interference detection channel; Determining the absorbance of the sample solution comprises: Controlling the light source device to emit detection incident light, wherein the detection incident light is normal incident light; Acquiring a detection signal of the detection output light, wherein the detection output light is the output light corresponding to the detection incident light after passing through the sample solution in the interference detection channel; The absorbance of the sample solution is determined according to the detection signal of the detected emitted light.

14. The method according to claim 13, characterized in that The detected incident light is normal incident light, wherein the normal incident light is incident light whose detection signal corresponding to the outgoing light after passing through the air is within the measurement range of the measuring device and satisfies M times of the full voltage range, wherein M is a positive real number greater than 0.5 and less than 1.

15. The method according to claim 8, characterized in that The sample analyzer includes a plurality of detection channels; the light source device is used to switch different wavelengths of the incident light during the detection process under the control of the processor; the method also includes: Determine a time segment of the target incident light in the incident light according to the detection items of the detection channel and the target incident light; According to the time segment, detection signals of the first emergent light and the second emergent light are determined.

16. The method according to claim 8, characterized in that After determining that the target incident light is the second incident light, acquiring the detection signal of the first outgoing light and acquiring the detection signal of the second outgoing light, the method further comprises: A detection signal of the first outgoing light and a detection signal of the second outgoing light are amplified.

17. A sample analyzer, characterized in that: include: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; A light source device, used for providing incident light with different light intensity values; A measuring device, used for detecting a detection signal of an outgoing light generated corresponding to the incident light passing through the reaction cup of the mixed solution; A processor is connected to the light source device and the measuring device, and is used to execute the method according to any one of claims 8 to 16.

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