Batch sample transfer system suitable for nucleic acid detection application and use method

Abstract

The invention discloses a batch sample transfer system suitable for nucleic acid detection application and a use method. The system comprises a main substrate, an uncapping tilting unit, an extraction plate treatment assembly, a transfer execution unit and a sampling tube group, by arranging two core function units, namely the uncapping and tilting unit and the transfer execution unit, uncapping, tilting, sample suction, station conversion and sample injection actions of batch sampling tubes are integrated, so that the pretreatment process of transferring samples in the sampling tubes to an extraction plate for nucleic acid samples is automatic, most manual operation is replaced, and the efficiency is improved. By integrating the inclination action of the test tube, the sampling rod section of the swab in the tube is attached to the surface, so that the possibility of needle collision is reduced to a great extent; according to the scheme, a single-row test tube group can be integrally put into the device, the working habits of workers are followed, the tedious operation of re-arranging test tubes in a test tube rack to a specified station one by one in the prior art is omitted, and the technical portability is effectively improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of nucleic acid sample transfer, and more specifically, relates to a batch sample transfer system suitable for nucleic acid detection applications and a method of use. Background Art

[0002] With the development of nucleic acid detection technology, nucleic acid detection has been widely used in various fields such as pathogen diagnosis, scientific research, and environmental monitoring. Nucleic acid detection is to find out whether there is nucleic acid of foreign invading viruses in the patient's respiratory specimens, blood or feces to determine whether the patient is infected with the virus. Therefore, once the nucleic acid test is "positive", it can be proved that the virus exists in the patient's body. After obtaining the test sample, it is generally necessary to open the lid, tilt the sample, close the lid, transfer the nucleic acid to the extraction plate, extract and analyze the nucleic acid, etc. At present, the pretreatment process is usually completed manually by the staff. When faced with large-scale tasks, work efficiency is difficult to guarantee. Therefore, a device that can replace manual labor to efficiently transfer samples in sampling tubes to extraction plates in batches is proposed, which is of substantial significance to the improvement of work efficiency.

[0003] In order to solve the above technical problems, Chinese invention patent CN113801770B discloses a nucleic acid detection pre-processing device, which includes a sterile isolation box, an extraction plate, a cup processing mechanism and a nucleic acid extraction mechanism. The extraction plate is arranged in the sterile isolation box; the cup processing mechanism is arranged in the sterile isolation box, and is used to divide the sample transported to the sterile isolation box into cups and sample on the extraction plate; the nucleic acid extraction mechanism is arranged in the sterile isolation box, and is used to extract nucleic acid from the sample on the extraction plate. The device can reduce the risk of infection and the protection level requirements for the detection personnel, and improve the processing efficiency. In addition, Chinese invention patent CN118356996A, a sample processing device and a nucleic acid detection integrated machine having the same, the sample processing device comprises: in the first horizontal direction, the transfer rack has a transfer position and a sample loading position, and the extraction rack has a placement position and an extraction position; the gripper is movable along a second horizontal direction perpendicular to the first horizontal direction, and the gripper can transfer the extraction plate to the transfer rack at the transfer position; the sample arm fills the sample or absorbs the nucleic acid to the extraction plate on the transfer rack at the sample loading position; the extraction rack moves to the placement position and the transfer rack moves to the transfer position, the gripper can transfer the extraction plate between the transfer rack and the extraction rack, the extraction rack moves to the extraction position, and the nucleic acid extraction part can extract nucleic acid from the extraction plate on the extraction rack; the sample processing device has a cache position arranged on the moving path of the gripper. The technical solution provided by the present application can solve the problem of low nucleic acid extraction efficiency of the sample processing device in the prior art.

[0004] The above patented technologies all use automated equipment to replace manual work, which improves the efficiency of nucleic acid sample processing to a certain extent. However, the following technical problems or areas for improvement still exist: (1) CN118356996A is provided with multiple robotic arm-like mechanisms, which relatively increase the manufacturing cost, making it difficult for small and medium-sized hospitals to accept it, and thus affecting its promotion. Moreover, according to the on-site application habits, a single-row test tube rack is usually used to arrange the sampling tubes storing nucleic acid samples. The above patented technologies do not incorporate this working habit into the design concept, discard the work experience of the staff, and require the test tubes in the test tube rack to be rearranged one by one to the designated workstations. The technology is not portable and also causes a waste of time. (1) When the syringe extracts the sample solution in the sampling tube, due to the presence of the sampling swab and the sampling rod segment inside the tube, the syringe needle and the sampling rod segment collide with each other, which easily causes system failure in the automated device. (3) After a single syringe extracts the sample from the sampling tube and transfers it to the extraction plate, it cannot be reused for a second time. However, the above patented technologies all provide a more convenient syringe replacement strategy. Summary of the invention

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a batch sample transfer system suitable for nucleic acid detection applications. By setting two core functional units, a capping and tilting unit and a transfer execution unit, the capping, tilting, sample suction, station conversion and sample injection actions of batch sampling tubes are integrated into one, thereby automating the nucleic acid sample pretreatment process of transferring samples in the sampling tubes to the extraction plate, replacing most of the manual operations. By integrating the test tube tilting action, the swab sampling rod section in the tube is attached to the surface, which greatly reduces the possibility of needle collision. This solution can place a single-row test tube group as a whole into the device, following the working habits of the staff, eliminating the tedious operation of rearranging the test tubes in the test tube rack to the designated stations one by one in the prior art, and effectively improving the portability of the technology.

[0006] In order to achieve the above object, according to a first aspect of the present invention, a batch sample transfer system suitable for nucleic acid detection applications comprises:

[0007] A main body consisting of a first stage, a second stage and a third stage, an open-cap tilting unit disposed on the first stage, an extraction plate handling assembly disposed on the second stage, a transfer execution unit disposed on the third stage, and a sampling tube group installed in the open-cap tilting unit; the sampling tube group includes a test tube rack and a sampling tube installed in the test tube rack;

[0008] The cap opening and tilting unit comprises a tilting support assembly fixedly connected to the upper surface of the first step, a pipe group mounting assembly rotatably connected to the output end of the tilting support assembly, and a pipe cap opening and closing assembly arranged directly above the pipe group mounting assembly; the tilting support assembly comprises a vertical support rod fixedly connected to the upper surface of the first step, a top positioning block arranged at the top of the vertical support rod, and a tilting drive motor installed on the side of the top positioning block;

[0009] The transfer execution unit includes a station conversion component rotatably connected to the third stage, an aspiration point control component and an aspiration volume control component arranged at the end of the station conversion component, and a sample aspirator installed and controlled by the aspiration point control component and the aspiration volume control component; the station conversion component includes a station conversion motor fixed to the front and rear sides of the third stage, a side connecting plate fixedly connected to the output end of the station conversion motor, and a loading base connected to the top of the side connecting plates on the front and rear sides, and the loading base is fixedly connected to the aspiration point control component and the aspiration volume control component.

[0010] Preferably, the pipe group installation assembly comprises:

[0011] An intermediate connecting plate fixedly connected to the output shaft of the tilting drive motor, a first connecting rod arranged below the intermediate connecting plate, and a tube group clamping body arranged at the lower end of the first connecting rod, wherein the tube group clamping body is provided with a tube group clamping groove for clamping the sampling tube group.

[0012] Preferably, the sampling tube comprises:

[0013] A pipe body and a cap assembly covering the pipe opening of the pipe body;

[0014] The pipe body includes an external thread arranged on the outer surface of the pipe mouth;

[0015] The cap body assembly includes a connecting base ring, an internal thread arranged on the inner surface of the connecting base ring and threadedly connected to the external thread, a cover body that is rotatably connected to one side of the connecting base ring through a rotating connecting part, a cover clamp assembly used for clamping connection between the cover body and the connecting base ring, a sealing film sealed at the ring mouth on the connecting base ring, an opening and closing auxiliary push clamp head arranged on the edge of the cover body on the opposite side of the rotating connecting part, and a clamping groove located between the opening and closing auxiliary push clamp head and the side edge of the cover body.

[0016] Preferably, the cover card assembly comprises:

[0017] A side convex clamping head and a side concave clamping groove are respectively arranged on the lower surface of the cover body and the upper surface of the connecting base ring, and the side convex clamping head and the side concave clamping groove can maintain an openable and closable clamping connection.

[0018] Preferably, the pipe cap opening and closing assembly comprises:

[0019] A top connecting plate on the upper edge of the middle connecting plates on the front and rear sides, an opening cover assembly arranged on the top connecting plate on the front side, and a closing cover assembly arranged on the top connecting plate on the rear side are respectively connected to the top connecting plate through a second connecting rod;

[0020] The cover opening assembly comprises a cover opening toggle servo fixed to the front middle connecting plate, a cover opening toggle rod fixedly connected to the output shaft of the cover opening toggle servo, a first arc-shaped slide groove opened on the front middle connecting plate along the motion track of the end of the cover opening toggle rod, and a transverse cover opening push rod fixed to the end of the cover opening toggle rod parallel to the direction of the tube group slot, and the end of the transverse cover opening push rod is kept in sliding connection with the first arc-shaped slide groove;

[0021] The sampling tube group is installed in a state where the sampling tube slot is stuck in the horizontal open cover push rod, and the open cover toggle servo is controlled to move upward, which drives the horizontal open cover push rod at the end of the open cover toggle rod to rotate along the first arc-shaped sliding groove, thereby lifting the sampling tube covers in the sampling tube group to realize batch opening.

[0022] Preferably, the cover assembly comprises:

[0023] A cover-closing toggle servo fixed to the middle connecting plate at the rear side, a cover-closing toggle rod fixedly connected to the cover-closing toggle servo, a second arc-shaped slide groove provided on the middle connecting plate at the rear side along the motion track of the end of the cover-closing toggle rod, and a transverse cover-closing push rod fixed to the end of the cover-closing toggle rod in parallel with the direction of the tube group clamping groove;

[0024] By controlling the cover closing toggle servo to move downward, the transverse cover closing push rod presses the cover body to close.

[0025] Preferably, the suction point control component comprises:

[0026] A first control motor fixed on one side of the loading base, a first slide groove opened on the surface of the loading base, a first control screw rod parallel to the first slide groove and coaxially fixedly connected to the first control motor, an intermediate nut block maintaining threaded transmission with the first control screw rod, a syringe clamp fixedly connected to the intermediate nut block, and an arc-shaped rubber clamping groove provided on the front section of the upper surface of the syringe clamping platform and used for clamping the sample syringe.

[0027] Preferably, the suction volume control component comprises:

[0028] A second control motor fixed to the rear side of the syringe clamp, a second slide groove opened on the upper surface of the rear section of the syringe clamp, a second control screw rod parallel to the second slide groove and coaxially fixedly connected to the second control motor, and a push handle clamping slider that maintains threaded transmission with the second control screw rod; the push handle clamping slider is used to clamp the push handle of the sample syringe.

[0029] Preferably, the extraction plate disposal assembly comprises:

[0030] A plate installation groove is arranged on the upper surface of the second step, and a row-type extraction plate is installed in the plate installation groove.

[0031] According to a second aspect of the present invention, a method for using a batch sample transfer system suitable for nucleic acid detection applications comprises the following steps:

[0032] S100: First, assemble the sampling tube to the tube assembly assembly;

[0033] S200: opening the caps of the test tubes in the sampling tube group as a whole by means of the tube cap opening and closing assembly, and controlling the test tube group to tilt at a fixed angle toward the transfer execution unit by means of the tilting support assembly, so that the swab sampling rod section is attached to the wall to prevent the needle from being hit;

[0034] S300: Control the sample aspirator to face the nozzle of the sampling tube group through the station conversion component, and control the aspiration point position and aspiration volume of the sample aspirator through the aspiration point control component and the aspiration volume control component respectively until the sample aspiration is completed;

[0035] S400: controlling the station conversion component to inject the sample in the sample aspirator into the extraction plate disposal component;

[0036] S500: Control the workstation conversion assembly to rotate in the reverse direction so that the needle of the sample aspirator faces upwards, so that the staff can replace the sample aspirator.

[0037] In general, the above technical solutions conceived by the present invention can achieve the following beneficial effects compared with the prior art:

[0038] 1. The present invention is a batch sample transfer system suitable for nucleic acid detection applications. By setting two core functional units, namely, a cap opening and tilting unit and a transfer execution unit, the cap opening, tilting, sample suction, station conversion and sample injection actions of batch sampling tubes are integrated into one, thereby automating the nucleic acid sample pre-treatment process of transferring samples in the sampling tubes to the extraction plate, replacing most manual operations. By integrating the test tube tilting action, the swab sampling rod section in the tube is attached to the surface, which greatly reduces the possibility of needle collision. This solution can put a single-row test tube group into the device as a whole, following the working habits of the staff, eliminating the cumbersome operation of re-arranging the test tubes in the test tube rack to the designated stations one by one in the prior art, and effectively improving the portability of the technology.

[0039] 2. The batch sample transfer system of the present invention is suitable for nucleic acid detection applications. During use, two people can cooperate, one person is responsible for replacing the test tube set, and the other person is responsible for replacing the sample aspirator and the extraction plate. It has a reasonable work allocation plan, standardizes the operation of the device, and is beneficial to the improvement of work quality and efficiency.

[0040] 3. A batch sample transfer system suitable for nucleic acid detection applications of the present invention integrates a station conversion component, an aspiration point position control component and an aspiration volume control component into one to form a transfer execution unit. Since the sample injector is installed in an arc-shaped rubber slot, during use, the station conversion motor is controlled to drive the aspiration point position control component and the aspiration volume control component to rotate, thereby realizing a change in the direction of the needle of the sample injector, that is, from a sampling station facing the sampling tube mouth to a sample discharge station facing the row extraction plate, or to a sample injector replacement station facing directly above; in addition, in the sampling station and the sample discharge station states, by controlling the first control motor and the second control motor, the sample injector can be pushed forward as a whole, and the aspiration volume and injection volume can be controlled, thereby improving the overall flexibility of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of the overall structure of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0042] Figure 2 This is a schematic diagram of the structure of a transfer execution unit of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0043] Figure 3 This is a schematic diagram of the structure of an open-cap tilting unit of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0044] Figure 4This is a schematic diagram of the structure of a sampling tube group of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0045] Figure 5 This is a schematic diagram of the sampling tube structure of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0046] Figure 6 A is a partial enlarged view of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0047] Figure 7 A partial enlarged view B of a batch sample transfer system suitable for nucleic acid detection application according to an embodiment of the present invention;

[0048] Figure 8 This is a schematic diagram of the cover opening principle structure of a batch sample transfer system suitable for nucleic acid detection applications in an embodiment of the present invention;

[0049] Fig. 9 This is a schematic diagram of the main structure of a cover opening component of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0050] Fig.10 This is a schematic diagram of the front view of the structure of a cover closing component of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0051] Fig.11 This is a schematic diagram of the structure of a row-type extraction plate of a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention;

[0052] Fig.12 The present invention is a flowchart of a method for using a batch sample transfer system suitable for nucleic acid detection applications according to an embodiment of the present invention.

[0053] In all the drawings, the same reference numerals represent the same technical features, specifically: 1-main body, 101-first step, 102-second step, 103-third step, 2-opening cap tilting unit, 210-tilting support assembly, 211-vertical support rod, 212-top positioning block, 213-tilting drive motor, 220-tube group installation assembly, 221-middle connecting plate, 222-first connecting rod, 223-tube group clamping body, 2231-tube group clamping slot, 2 30-pipe cap opening and closing assembly, 231-second connecting rod, 232-top connecting plate, 233-first arc-shaped slide, 234-opening cover toggle servo, 235-opening cover toggle rod, 236-lateral opening cover push rod, 237-closing cover toggle servo, 238-closing cover toggle rod, 239-second arc-shaped slide, 2310-lateral closing cover push rod, 3-transfer execution unit, 310-station conversion assembly, 311-station conversion motor, 312-side connecting plate, 313-loading base Table, 320-injection point control component, 321-first control motor, 322-first control screw, 323-middle nut block, 324-injection clamp, 3241-arc rubber slot, 330-injection volume control component, 331-second control motor, 332-second control screw, 333-push handle clamping slider, 340-sample injector, 341-main cavity, 342-push handle, 343-needle, 4-extraction plate disposal component, 401-plate body installation Mounting slot, 410-row extraction plate, 5-sampling tube group, 500-test tube rack, 510-sampling tube, 511-tube body, 5111-external thread, 512-cap body assembly, 5121-connecting base ring, 5122-internal thread, 5123-rotating connection part, 5124-cover body, 5125-opening and closing auxiliary push clamp, 5126-cage, 5127-sealing film, 5128-cover clamp assembly, 51281-side convex clamp, 51282-side concave slot. DETAILED DESCRIPTION

[0054] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0055] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0056] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0057] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0058] like Figures 1 to 11 As shown, in an embodiment of the present invention, the batch sample transfer system suitable for nucleic acid detection application includes:

[0059] A main base 1 consisting of a first stage 101, a second stage 102 and a third stage 103, an open-cap tilting unit 2 disposed on the first stage 101, an extraction plate handling assembly 4 disposed on the second stage 102, a transfer execution unit 3 disposed on the third stage 103, and a sampling tube group 5 installed in the open-cap tilting unit 2; the sampling tube group 5 includes a test tube rack 500 and a sampling tube 510 installed in the test tube rack 500;

[0060] The cap opening and tilting unit 2 comprises a tilting support assembly 210 fixedly connected to the upper surface of the first step 101, a tube group mounting assembly 220 rotatably connected to the output end of the tilting support assembly 210, and a tube cap opening and closing assembly 230 arranged directly above the tube group mounting assembly 220; the tilting support assembly 210 comprises a vertical support rod 211 fixedly connected to the upper surface of the first step 101, a top positioning block 212 arranged at the top of the vertical support rod 211, and a tilting drive motor 213 installed on the side of the top positioning block 212;

[0061] The transfer execution unit 3 includes a station conversion component 310 rotatably connected to the third stage 103, an aspiration point control component 320 and an aspiration volume control component 330 arranged at the end of the station conversion component 310, and a sample aspirator 340 installed and controlled by the aspiration point control component 320 and the aspiration volume control component 330; the station conversion component 310 includes a station conversion motor 311 fixed to the front and rear sides of the third stage 103, a side connecting plate 312 fixedly connected to the output end of the station conversion motor 311, and a loading base 313 connected to the top of the side connecting plates 312 at the front and rear sides, and the loading base 313 is fixedly connected to the aspiration point control component 320 and the aspiration volume control component 330;

[0062] The sampling tube group 5 is installed on the tube group installation component 220, and the cap of the test tube in the sampling tube group 5 is opened as a whole by means of the tube cap opening and closing component 230. The tilting support component 210 is used to control the test tube group to tilt at a fixed angle toward the transfer execution unit 3 so that the swab sampling rod section can stick to the wall to prevent the needle from hitting the needle. Subsequently, the sample aspirator 340 is controlled to face the tube mouth of the sampling tube group 5 through the workstation conversion component 310, and the aspiration point position and aspiration volume of the sample aspirator 340 are controlled by the aspiration point control component 320 and the aspiration volume control component 330 respectively. Finally, the workstation conversion component 310 is controlled again to inject the sample in the sample aspirator 340 into the extraction plate disposal component 4.

[0063] In the embodiment of the present invention, by setting two core functional units, namely the cap opening and tilting unit 2 and the transfer execution unit 3, the cap opening, tilting, sample suction, station conversion and sample injection actions of the batch sampling tubes are integrated into one, thereby automating the nucleic acid sample pretreatment process of transferring the samples in the sampling tubes to the extraction plate, replacing most of the manual operations, and by integrating the test tube tilting action, the swab sampling rod section in the tube is attached to the surface, which greatly reduces the possibility of needle collision; this solution can place a single-row test tube group into the device as a whole, following the working habits of the staff, eliminating the tedious operation of the prior art of rearranging the test tubes in the test tube rack to the designated stations one by one, and the technical portability is effectively improved.

[0064] In addition, the use process can be carried out by two people, one person is responsible for replacing the test tube set, and the other person is responsible for replacing the sample aspirator and the extraction plate. It has a reasonable work distribution plan and standardizes the operation of the device, which is beneficial to improving work quality and efficiency.

[0065] like Figure 1 and Figure 3 As shown, in an embodiment of the present invention, the pipe group installation assembly 220 includes:

[0066] An intermediate connecting plate 221 fixedly connected to the output shaft of the tilting drive motor 213, a first connecting rod 222 arranged below the intermediate connecting plate 221, and a tube group clamping body 223 arranged at the lower end of the first connecting rod 222, wherein the tube group clamping body 223 is provided with a tube group clamping groove 2231 for clamping the sampling tube group 5.

[0067] like Figure 4 to Figure 7 As shown, in this embodiment of the present invention, the sampling tube 510 includes:

[0068] A pipe body 511 and a cap assembly 512 covering the pipe opening of the pipe body 511;

[0069] The pipe body 511 includes an external thread 5111 provided on the outer surface of the pipe mouth;

[0070] The cap body assembly 512 includes a connecting base ring 5121, an internal thread 5122 arranged on the inner surface of the connecting base ring 5121 and threadedly connected to the external thread 5111, a cover body 5124 that is rotatably connected to one side of the connecting base ring 5121 through a rotating connection part 5123, a cover clamp assembly 5128 for clamping connection between the cover body 5124 and the connecting base ring 5121, a sealing film 5127 sealed at the ring mouth of the connecting base ring 5121, an opening and closing auxiliary push clamp 5125 on the opposite side of the rotating connection part 5123 and arranged on the edge of the cover body 5124, and a clamping groove 5126 located between the opening and closing auxiliary push clamp 5125 and the side of the cover body 5124.

[0071] like Figure 6 and Figure 7 As shown, in this embodiment of the present invention, the cover card assembly 5128 includes:

[0072] The lateral convex clamping head 51281 and the lateral concave clamping groove 51282 are respectively arranged on the lower surface of the cover body 5124 and the upper surface of the connecting base ring 5121, and the lateral convex clamping head 51281 and the lateral concave clamping groove 51282 maintain an openable and closable clamping connection.

[0073] like Figure 1 , Figure 3 , Figure 6 , Figure 8 to Figure 10 As shown, in the embodiment of the present invention, the pipe cap opening and closing assembly 230 includes:

[0074] A top connecting plate 232 connected to the upper edge of the middle connecting plates 221 at the front and rear sides, an opening cover assembly provided on the front top connecting plate 232, and a closing cover assembly provided on the rear top connecting plate 232 are respectively connected through a second connecting rod 231;

[0075] The cover opening assembly includes a cover opening toggle servo 234 fixed to the front middle connecting plate 221, a cover opening toggle rod 235 fixedly connected to the output shaft of the cover opening toggle servo 234, a first arc-shaped slide groove 233 opened on the front middle connecting plate 221 along the motion track of the end of the cover opening toggle rod 235, and a transverse cover opening push rod 236 fixed to the end of the cover opening toggle rod 235 in parallel with the direction of the tube group slot 2231, and the end of the transverse cover opening push rod 236 is kept in sliding connection with the first arc-shaped slide groove 233;

[0076] The sampling tube group 5 is installed in a state where the sampling tube 510 slot 5126 is stuck in the horizontal open cover push rod 236, and the open cover toggle servo 234 is controlled to move upward, which drives the horizontal open cover push rod 236 at the end of the open cover toggle rod 235 to rotate along the first arc-shaped slide groove 233, thereby lifting the cover body 5124 of the sampling tube 510 in the sampling tube group 5, thereby realizing batch opening of the cover.

[0077] like Figure 1 , Figure 3 , Figure 6 , Figure 8 to Figure 10 As shown, in an embodiment of the present invention, the cover assembly includes:

[0078] A cover closing toggle servo 237 fixed to the middle connecting plate 221 at the rear side, a cover closing toggle rod 238 fixedly connected to the cover closing toggle servo 237, a second arc-shaped slide groove 239 provided on the middle connecting plate 221 at the rear side along the motion track of the end of the cover closing toggle rod 238, and a transverse cover closing push rod 2310 fixed to the end of the cover closing toggle rod 238 in parallel with the direction of the tube group clamping groove 2231;

[0079] By controlling the cover closing toggle servo 237 to move downward, the transverse cover closing push rod 2310 presses the cover body 5124 to close.

[0080] like Figure 1 and Figure 2 As shown, in this embodiment of the present invention, the suction point control component 320 includes:

[0081] A first control motor 321 fixed to one side of the loading base 313, a first slide groove opened on the surface of the loading base 313, a first control screw rod 322 parallel to the first slide groove and coaxially fixedly connected to the first control motor 321, an intermediate nut block 323 maintaining threaded transmission with the first control screw rod 322, a syringe clamp 324 fixedly connected to the intermediate nut block 323, and an arc-shaped rubber groove 3241 provided on the front section of the upper surface of the syringe clamp 324 and used to clamp the sample syringe 340.

[0082] like Figure 1 and Figure 2 As shown, in this embodiment of the present invention, the suction volume control component 330 includes:

[0083] A second control motor 331 fixed to the rear side of the syringe clamp 324, a second slide groove opened on the upper surface of the rear section of the syringe clamp 324, a second control screw rod 332 parallel to the second slide groove and coaxially fixedly connected to the second control motor 331, and a push handle clamping slider 333 that maintains threaded transmission with the second control screw rod 332; the push handle clamping slider 333 is used to clamp the push handle 342 of the sample syringe.

[0084] In the embodiment of the present invention, the transfer execution unit 3 is formed by integrating the station conversion component 310, the suction point control component 320 and the suction volume control component 330. Since the sample injector is installed in the arc-shaped rubber card groove 3241, during use, the station conversion motor 311 is controlled to drive the suction point control component 320 and the suction volume control component 330 to rotate, so as to realize the change of the direction of the needle of the sample injector, that is, from the sampling station facing the sampling tube mouth to the sample injection station facing the row extraction plate, or to the sample injector replacement station facing directly above; in addition, in the sampling station and the sample injection station state, by controlling the first control motor 321 and the second control motor 331, the sample injector 340 can be pushed forward as a whole, and the suction volume and injection volume can be controlled, thereby improving the overall flexibility of the device.

[0085] like Figure 1 As shown, in an embodiment of the present invention, the extraction plate disposal assembly 4 comprises:

[0086] A plate mounting groove 401 is provided on the upper surface of the second step 102 , and a row-type extraction plate 410 is installed in the plate mounting groove 401 .

[0087] like Fig.12 As shown, in an embodiment of the present invention, a method for using a batch sample transfer system suitable for nucleic acid detection applications includes the following steps:

[0088] S100: First, install the sampling tube set 5 on the tube set installation assembly 220;

[0089] S200: opening the caps of the test tubes in the sampling tube group 5 as a whole by means of the tube cap opening and closing assembly 230, and controlling the test tube group to tilt toward the transfer execution unit 3 at a fixed angle by using the tilting support assembly 210, so that the swab sampling rod section is attached to the wall to prevent the needle from being hit;

[0090] S300: Control the sample aspirator 340 to face the tube opening of the sampling tube group 5 through the station conversion component 310, and control the aspiration point position and aspiration volume of the sample aspirator 340 through the aspiration point control component 320 and the aspiration volume control component 330 respectively until the sample aspiration is completed;

[0091] S400: Control the workstation conversion component 310 to inject the sample in the sample injector 340 into the extraction plate disposal component 4;

[0092] S500: Control the workstation conversion assembly 310 to rotate in the reverse direction so that the needle of the sample aspirator 340 faces upwards, so that the staff can replace the sample aspirator.

[0093] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. The above are only preferred implementations of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. A batch sample transfer system suitable for nucleic acid detection applications, characterized in that: include: A main base (1) consisting of a first stage (101), a second stage (102) and a third stage (103), a cap opening and tilting unit (2) arranged on the first stage (101), an extraction plate handling assembly (4) arranged on the second stage (102), a transfer execution unit (3) arranged on the third stage (103), and a sampling tube group (5) installed in the cap opening and tilting unit (2); the sampling tube group (5) comprises a test tube rack (500) and a sampling tube (510) installed in the test tube rack (500); The cap opening and tilting unit (2) comprises a tilting support assembly (210) fixedly connected to the upper surface of the first step (101), a tube group mounting assembly (220) rotatably connected to the output end of the tilting support assembly (210), and a tube cap opening and closing assembly (230) arranged directly above the tube group mounting assembly (220); the tilting support assembly (210) comprises a vertical support rod (211) fixedly connected to the upper surface of the first step (101), a top positioning block (212) arranged at the top of the vertical support rod (211), and a tilting drive motor (213) installed on the side of the top positioning block (212); The transfer execution unit (3) comprises a station conversion component (310) rotatably connected to the third stage (103), an aspiration point control component (320) and an aspiration volume control component (330) arranged at the end of the station conversion component (310), and a sample aspirator (340) installed and controlled by the aspiration point control component (320) and the aspiration volume control component (330); the station conversion component (310) comprises a station conversion motor (311) fixed to the front and rear sides of the third stage (103), a side connecting plate (312) fixedly connected to the output end of the station conversion motor (311), and a loading base (313) connected to the top of the side connecting plates (312) at the front and rear sides, and the loading base (313) is fixedly connected to the aspiration point control component (320) and the aspiration volume control component (330).

2. A batch sample transfer system suitable for nucleic acid detection applications according to claim 1, characterized in that: The pipe assembly installation component (220) comprises: An intermediate connecting plate (221) fixedly connected to the output shaft of the tilting drive motor (213), a first connecting rod (222) arranged below the intermediate connecting plate (221), and a tube group clamping body (223) arranged at the lower end of the first connecting rod (222), wherein the tube group clamping body (223) is provided with a tube group clamping groove (2231) for clamping the sampling tube group (5).

3. A batch sample transfer system suitable for nucleic acid detection applications according to claim 1, characterized in that: The sampling tube (510) comprises: A pipe body (511), and a cap body assembly (512) covering the pipe opening of the pipe body (511); The pipe body (511) includes an external thread (5111) provided on the outer surface of the pipe opening; The cap body assembly (512) comprises a connecting base ring (5121), an internal thread (5122) provided on the inner surface of the connecting base ring (5121) and threadedly connected to the external thread (5111), a cover body (5124) rotatably connected to one side of the connecting base ring (5121) via a rotating connecting portion (5123), a cover clamping assembly (5128) for clamping connection between the cover body (5124) and the connecting base ring (5121), a sealing film (5127) sealed at the ring opening of the connecting base ring (5121), an opening and closing auxiliary push clamp (5125) on the opposite side of the rotating connecting portion (5123) and provided on the edge of the cover body (5124), and a clamping groove (5126) located between the opening and closing auxiliary push clamp (5125) and the side edge of the cover body (5124).

4. A batch sample transfer system suitable for nucleic acid detection applications according to claim 3, characterized in that: The cover card assembly (5128) comprises: A lateral convex clamping head (51281) and a lateral concave clamping groove (51282) are respectively arranged on the lower surface of the cover body (5124) and the upper surface of the connecting base ring (5121), and the lateral convex clamping head (51281) and the lateral concave clamping groove (51282) maintain an openable and closable clamping connection.

5. A batch sample transfer system suitable for nucleic acid detection applications according to claim 4, characterized in that: The pipe cap opening and closing assembly (230) comprises: A top connecting plate (232) connected to the upper edges of the front and rear middle connecting plates (221) respectively through a second connecting rod (231), a cover opening component arranged on the front top connecting plate (232), and a cover closing component arranged on the rear top connecting plate (232); The cover opening assembly comprises a cover opening toggle servo (234) fixed to the front middle connecting plate (221), a cover opening toggle rod (235) fixedly connected to the output shaft of the cover opening toggle servo (234), a first arc-shaped sliding groove (233) provided on the front middle connecting plate (221) along the motion track of the end of the cover opening toggle rod (235), and a transverse cover opening push rod (236) fixed to the end of the cover opening toggle rod (235) parallel to the direction of the tube group clamping groove (2231), and the end of the transverse cover opening push rod (236) is kept in sliding connection with the first arc-shaped sliding groove (233); The sampling tube group (5) is installed in a state where the sampling tube (510) clamping groove (5126) is clamped in the transverse cover opening push rod (236), and the cover opening toggle servo (234) is controlled to move upward, thereby driving the transverse cover opening push rod (236) at the end of the cover opening toggle rod (235) to rotate along the first arc-shaped sliding groove (233), and the cover body (5124) of the sampling tube (510) in the sampling tube group (5) is lifted, thereby realizing batch opening of the covers.

6. A batch sample transfer system suitable for nucleic acid detection applications according to claim 5, characterized in that: The cover assembly comprises: A cover closing toggle steering gear (237) fixed to the middle connecting plate (221) at the rear side, a cover closing toggle rod (238) fixedly connected to the cover closing toggle steering gear (237), a second arc-shaped sliding groove (239) provided on the middle connecting plate (221) at the rear side along the motion track of the end of the cover closing toggle rod (238), and a transverse cover closing push rod (2310) fixed to the end of the cover closing toggle rod (238) in parallel with the direction of the tube group clamping groove (2231); By controlling the cover closing steering engine (237) to move downward, the transverse cover closing push rod (2310) presses the cover body (5124) to close.

7. A batch sample transfer system suitable for nucleic acid detection applications according to claim 1, characterized in that: The injection point control component (320) comprises: A first control motor (321) fixed to one side of the loading base (313), a first slide groove opened on the surface of the loading base (313), a first control screw (322) parallel to the first slide groove and coaxially fixedly connected to the first control motor (321), an intermediate nut block (323) maintaining threaded transmission with the first control screw (322), a syringe clamp (324) fixedly connected to the intermediate nut block (323), and an arc-shaped rubber clamping groove (3241) provided on the front section of the upper surface of the syringe clamp (324) and used for clamping the sample syringe (340).

8. A batch sample transfer system suitable for nucleic acid detection applications according to claim 7, characterized in that: The suction volume control component (330) comprises: A second control motor (331) fixed to the rear side of the syringe clamp (324), a second slide groove opened on the upper surface of the rear section of the syringe clamp (324), a second control screw (332) parallel to the second slide groove and coaxially fixedly connected to the second control motor (331), and a push handle clamping slider (333) threadedly driven with the second control screw (332); the push handle clamping slider (333) is used to clamp the push handle (342) of the sample syringe.

9. A batch sample transfer system suitable for nucleic acid detection applications according to any one of claims 1 to 8, characterized in that: The extraction plate handling assembly (4) comprises: A plate mounting groove (401) is provided on the upper surface of the second step (102), and a row-type extraction plate (410) is installed in the plate mounting groove (401).

10. A method for using a batch sample transfer system suitable for nucleic acid detection applications according to claim 9, comprising the following steps: S100: First, install the sampling tube set (5) on the tube set installation assembly (220); S200: opening the cap of the test tube in the sampling tube group (5) as a whole by means of the tube cap opening and closing assembly (230), and controlling the test tube group to tilt at a fixed angle toward the transfer execution unit (3) by means of the tilting support assembly (210), so that the swab sampling rod section is attached to the wall to prevent the needle from being hit; S300: Control the sample aspirator (340) to face the tube opening of the sampling tube group (5) through the workstation conversion component (310), and control the aspiration point position and aspiration volume of the sample aspirator (340) through the aspiration point control component (320) and the aspiration volume control component (330) respectively until the sample aspiration is completed; S400: Controlling the workstation conversion component (310) to inject the sample in the sample aspirator (340) into the extraction plate disposal component (4); S500: Control the workstation conversion assembly (310) to rotate in the reverse direction so that the needle of the sample aspirator (340) faces upwards, so that the staff can replace the sample aspirator.

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