Breeding method of Taiwan chenopodium quinoa

Abstract

The invention discloses a Taiwan chenopodium quinoa breeding method, and relates to the technical field of introduction and breeding of Taiwan chenopodium quinoa. The method comprises the following steps: S1, obtaining Taiwan chenopodium quinoa seeds, and controlling the water content; s2, carrying out sterilization treatment on the obtained seeds; s3, preparing a first bacterial solution by adopting bacillus subtilis and bacillus licheniformis, and culturing the seeds for the first time; s4, bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus, actinomycetes, trichoderma harzianum and photosynthetic bacteria are adopted to prepare a second bacterial solution, and the seeds are subjected to secondary seed culture; by breeding the Taiwan chenopodium quinoa seeds, the Taiwan chenopodium quinoa seeds can be massively planted in a mainland soil environment, the germination rate is increased when the Taiwan chenopodium quinoa seeds are planted in the mainland soil, meanwhile, rhizobium in the soil is inhibited, breeding of nematode eggs is avoided, the soil environment is improved in the long-term planting process, and the survival rate of the Taiwan chenopodium quinoa is increased. And heavy metals such as mercury and cadmium in the soil are continuously decomposed.

Description

Technical Field

[0001] The invention relates to the technical field of introduction and breeding of Taiwan red quinoa, in particular to a breeding method of Taiwan red quinoa. Background Art

[0002] Taiwan red quinoa is a grain plant of the Amaranthaceae family native to the high mountainous areas of southeastern Taiwan, China, and is well-known for its high nutritional value. Taiwan red quinoa is rich in calcium, dietary fiber, amino acids and proteins that cannot be synthesized naturally by the human body, vitamins, minerals and antioxidants. It is a whole plant protein source suitable for vegetarians and people who need a high-protein diet.

[0003] Among them, red quinoa native to the Andes Mountains in South America has been introduced into Northwest my country, such as Gansu, Shaanxi, Qinghai and other places for breeding and cultivation, so that it can adapt to the growth environment of my country's saline-alkali land and arid land, and the introduction and breeding has been successful.

[0004] However, compared with Taiwan red quinoa, the nutritional value and health benefits of Northwest red quinoa are inferior to those of Taiwan red quinoa. The protein content of Taiwan red quinoa is as high as 17.7%, which is twice that of wheat, and the dietary fiber content is 15 times that of ordinary white rice. It is rich in nutrients such as calcium, iron, and amino acids. It also has many health benefits such as regulating blood sugar, lowering cholesterol, protecting the heart, and losing weight.

[0005] Northwestern red quinoa and Taiwan red quinoa are two plants of the same family but different species. They have obvious differences in germplasm resources and genetic background. Therefore, how to breed Taiwan red quinoa so that it can adapt to the soil environment of the mainland and complete the introduction and localization of the plant is the key to allowing Taiwan red quinoa to be planted as a crop in the mainland. Summary of the invention

[0006] The purpose of the present invention is to provide a breeding method for Taiwan red quinoa, which can achieve the purpose of enabling Taiwan red quinoa seeds to be planted in large quantities in a continental soil environment and improving the germination rate when planted in continental soil by breeding Taiwan red quinoa seeds.

[0007] In order to achieve the above object, the present invention adopts the following technical means: A method for breeding Taiwanese red quinoa comprises the following steps: S1. Obtain Taiwan red quinoa seeds and control the water content; S2. sterilize the obtained seeds; S3. The first bacterial solution is prepared by using Bacillus subtilis and Bacillus licheniformis to cultivate the seeds for the first time; S4. A second bacterial solution is prepared using Bacillus subtilis, Bacillus licheniformis, Bacillus gelatinosa, Actinomycetes, Trichoderma harzianum and photosynthetic bacteria, and the seeds are cultured for the second time.

[0008] Preferably, in step S1, the moisture content of Taiwan red quinoa seeds is controlled to 20% by natural drying.

[0009] Furthermore, in step S2, sulfur is used to perform fumigation sterilization on Taiwanese red quinoa seeds for 10 days.

[0010] Furthermore, the first bacterial liquid is made of 30% of Bacillus subtilis and 20% of Bacillus licheniformis based on the weight of the seeds.

[0011] Furthermore, the first cultivation lasts for 15 days.

[0012] Furthermore, the second bacterial liquid is made of 10% Bacillus subtilis, 10% Trichoderma harzianum, 10% Bacillus licheniformis, 10% Bacillus gelatinosa, 10% Actinomycetes and 10% photosynthetic bacteria based on the weight of the seeds.

[0013] Furthermore, the second cultivation lasts for 15 days.

[0014] Furthermore, after the second breeding is completed, the bred seeds are directly sown or vacuum-preserved.

[0015] During use, the present invention has the following beneficial effects: First, by controlling the water content, ensure that the first bacterial solution and the second bacterial solution can be inhaled in appropriate amounts during subsequent seed cultivation. In the subsequent seed cultivation process, the first bacterial solution is used to cultivate the seeds, on the one hand, so that the seeds can absorb the probiotics. On the other hand, the most important thing is to use the first bacterial solution to open up the nutritional channel for the Taiwan red quinoa seeds to enter the embryo, enhance the vitality of the seeds, and facilitate the second seed cultivation. After the first cultivation is completed, the second bacterial solution is combined for the second cultivation. During the second cultivation, the added jelly-like Bacillus, actinomycetes and photosynthetic bacteria are used to improve the adaptability of the seeds to the mainland soil. At the same time, the added strains can enter the embryo through the nutritional channel formed by the first cultivation. After the seeds germinate, the strains can also be released to improve the soil environment. In the process of the second cultivation, the added Bacillus subtilis and Bacillus licheniformis are used to act as a carrier to increase the power of other strains to enter the seeds through the nutritional channel. After a series of breeding measures, this application can not only successfully cultivate Taiwan red quinoa seeds under mainland soil conditions, but also promote the seed germination rate to 90%~95%. After the seeds germinate, the released bacteria can also improve the plant's cold and drought resistance, inhibit rhizobia and avoid the breeding of nematode eggs. After the seeds germinate, they can also improve the soil environment and continuously decompose heavy metals such as mercury and cadmium in the soil. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0017] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features of the embodiments may be combined with each other.

[0019] A method for breeding Taiwanese red quinoa comprises the following steps: S1. Obtain Taiwan red quinoa seeds and control the moisture content; use natural drying to control the moisture content of Taiwan red quinoa seeds to 20%.

[0020] S2. Sterilize the obtained seeds; use sulfur to smoke-sterilize the Taiwan red quinoa seeds for 10 days.

[0021] S3. A first bacterial solution is prepared using Bacillus subtilis and Bacillus licheniformis, and the seeds are cultured for the first time; wherein the first culture lasts for 15 days.

[0022] S4. A second bacterial solution is prepared using Bacillus subtilis, Bacillus licheniformis, Bacillus gelatinosa, Actinomycetes, Trichoderma harzianum and photosynthetic bacteria, and the seeds are cultured for a second time, wherein the second culture lasts for 15 days.

[0023] After the second breeding is completed, the bred seeds can be directly sown or vacuum-packed for preservation.

[0024] The following describes it in detail in conjunction with specific implementation methods. Example

[0025] In this embodiment, the first bacterial liquid is made of 30% of Bacillus subtilis and 20% of Bacillus licheniformis based on the weight of the seeds.

[0026] The second bacterial liquid is prepared by 10% of Bacillus subtilis, 10% of Trichoderma harzianum, 10% of Bacillus licheniformis, 10% of Bacillus gelatinus, 10% of Actinomycetes and 10% of photosynthetic bacteria based on the weight of the seeds. Example

[0027] In this embodiment, the first seed cultivation step is removed, and the second bacterial solution is directly used for seed cultivation, wherein the second bacterial solution is made of 10% Bacillus subtilis, 10% Trichoderma harzianum, 10% Bacillus licheniformis, 10% Bacillus gelatinus, 10% Actinomycetes and 10% photosynthetic bacteria, accounting for 10% of the weight of the seeds. Example

[0028] In this embodiment, the Taiwan red quinoa seeds are dried in an oven, and the moisture content of the seeds is also controlled to be 20%. Then, the first bacterial liquid and the second bacterial liquid are used for the first and second cultivation, respectively, wherein the components of the first bacterial liquid and the second bacterial liquid are the same as those in Example 1.

[0029] In the breeding process of all the aforementioned embodiments, when carrying out the first breeding or the second breeding, all the seeds are completely soaked, and then a corresponding amount of bacteria is added, and then the mixture is stirred evenly before breeding.

[0030] Example 1 is the best example. When planted in continental soil, the germination rate can reach 90% to 95%, and the growth state is good. The germinated plants can have good cold and drought resistance, and can inhibit rhizobia and control the breeding of nematode eggs.

[0031] In Example 2, only the second seeding was carried out, and a large amount of bacterial liquid could only coat the surface of the seeds, making it impossible to carry out breeding improvement normally. The germination rate of Taiwan quinoa red quinoa seeds was basically difficult to adapt to the mainland soil environment, and its germination rate was only about 50%. Moreover, the germinated plants could not effectively restrain the growth of rhizobia and nematode eggs during their growth, which was a case of breeding failure.

[0032] For Example 3, since the drying is carried out by drying in a drying oven, even if the water content is also 20%, the drying in the oven will cause a large amount of seed embryos to be inactivated. Therefore, under the premise of adopting two breedings, the germination rate of seeds is only about 65%. Although the germinated plants also have the ability to resist cold and drought during the planting and cultivation process, and are good at avoiding root nodules and nematodes. However, due to the low germination rate, it is also not conducive to large-scale low-cost planting.

[0033] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for breeding Taiwanese red quinoa, characterized in that: The following steps are involved: S1. Obtain Taiwan red quinoa seeds and control the water content; S2. sterilize the obtained seeds; S3. The first bacterial solution is prepared by using Bacillus subtilis and Bacillus licheniformis to cultivate the seeds for the first time; S4. A second bacterial solution is prepared using Bacillus subtilis, Bacillus licheniformis, Bacillus gelatinosa, Actinomycetes, Trichoderma harzianum and photosynthetic bacteria, and the seeds are cultured for the second time.

2. The method for breeding Taiwanese red quinoa according to claim 1, characterized in that: In the step S1, the moisture content of the Taiwan red quinoa seeds is controlled to 20% by natural drying.

3. The method for breeding Taiwanese red quinoa according to claim 1, characterized in that: In the step S2, sulfur is used to perform fumigation sterilization on Taiwan red quinoa seeds for 10 days.

4. The method for breeding Taiwan red quinoa according to claim 1, characterized in that: The first bacterial liquid is made of 30% of Bacillus subtilis and 20% of Bacillus licheniformis based on the weight of the seeds.

5. A Taiwan red quinoa breeding method according to claim 1 or 4, characterized in that: The first cultivation lasted 15 days.

6. The method for breeding Taiwanese red quinoa according to claim 1, characterized in that: The second bacterial liquid is prepared from 10% of Bacillus subtilis, 10% of Trichoderma harzianum, 10% of Bacillus licheniformis, 10% of Bacillus gelatinus, 10% of Actinomycetes and 10% of photosynthetic bacteria, based on the weight of the seeds.

7. A Taiwan red quinoa breeding method according to claim 1 or 6, characterized in that: The second cultivation lasted 15 days.

8. The method for breeding Taiwan red quinoa according to claim 1, characterized in that: After the second breeding is completed, the bred seeds can be directly sown or vacuum-packed for preservation.