Insecticidal composition containing flupyradifurone and application thereof

Abstract

The invention relates to an insecticidal composition containing flupyradifurone and application thereof, and belongs to the technical field of pesticides, the insecticidal composition contains flupyradifurone, beta-cyfluthrin, basil extract, salvia officinalis extract, origanum vulgare extract and other components, and through the synergistic effect of different action mechanisms, the insecticidal composition has an excellent insecticidal effect; the flupyradifurone, the basil extract, the salvia officinalis extract and the origanum vulgare extract act together, and compared with single use of one component, after compounding, the intensity of effects such as poison killing and repelling on pests is improved, and the risk that the pests generate drug resistance to a single component is reduced. The extract component can enhance the poisoning effect of the flupyradifurone on pests, influence the metabolism of the pests on the flupyradifurone, and enhance the toxicity of the flupyradifurone in the pests. The dosage of the flupyradifurone is effectively reduced. The quick-acting property is good, and the medication period is shortened; the problems of resistance and poor imitation effect are solved, the composition is safe to crops, the content of flupyradifurone is reduced, and environmental pollution is relieved.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to an insecticidal composition containing flupyrazone and application thereof. Background Art

[0002] Flupyralidone is an insecticide ingredient that is suitable for many crops and is widely used in vegetables (such as tomatoes, peppers, cucumbers, eggplants, potatoes, etc.), fruit trees (such as citrus, apples, pears, etc.), coffee, cocoa, cotton, soybeans and other field crops.

[0003] Fluopyramide is mainly used to control pests such as aphids, whiteflies, whiteflies, leafhoppers, psyllids, and thrips. Aphids are common pests of crops. They suck crop sap, causing poor crop growth, curling and yellowing of leaves. Fluopyramide has a good control effect on aphids, can quickly control the aphid population, and protect the healthy growth of crops. Pests such as whiteflies and whiteflies not only suck plant sap, but also secrete honeydew, causing diseases such as sooty mold, affecting the photosynthesis and quality of crops; Fluopyramide can effectively control whiteflies and reduce their harm to crops. Leafhoppers feed on plant sap, and their feeding behavior will cause white spots and yellowing on plant leaves, affecting the yield and quality of crops; the use of fluopyramide can effectively control leafhoppers. Psyllids often harm crops such as fruit trees, and thrips occur on a variety of crops, which will have adverse effects on the growth and development of crops; Fluopyramide also has a good control effect on these pests.

[0004] In agricultural production, due to the long-term use of traditional pesticides, some pests have developed resistance to commonly used insecticides, resulting in a decrease in control effects. Flupyralidone has a new chemical structure and a unique mechanism of action. It has no cross-resistance with conventional neonicotinoid insecticides and still has control effects on pests that have developed resistance to other insecticides. However, as a pesticide, flupyralidone still has the following problems:

[0005] 1. Flupyralidone is considered a potential alternative to neonicotinoid pesticides. Although it has low acute toxicity to bees, chronic exposure can cause serious harm to bees. Chronic exposure to this pesticide can significantly reduce bee survival rate, weight gain and food utilization, affect bees' sucrose sensitivity and memory ability, cause oxidative stress, immunity and detoxification inhibition, and cause damage to bee brain and intestinal cells, including cell apoptosis and autophagy characteristics. This will have an adverse impact on bees' survival, reproduction, and pollination of crops.

[0006] 2. Although flupyralidone has no cross-resistance with conventional neonicotinoid insecticides, as its use time and frequency increase, pests will still develop a certain resistance to the agent, which will reduce the control effect of pesticides and increase the difficulty and cost of control.

[0007] 3. Flupyralidone residues in the environment may have potential impacts on the ecological environment such as soil and water. Although the agent has relatively little environmental pollution under natural conditions, after long-term and large-scale use, its accumulation in the environment may produce certain ecotoxicity to soil microbial communities, aquatic organisms, etc., affecting the balance and stability of the ecosystem.

[0008] Therefore, there is a need to continuously develop and prepare more efficient and safer insecticides to reduce the dosage of flupyrazone and reduce the impact of flupyrazone on bees and the environment. Summary of the invention

[0009] In view of the above-mentioned problems existing in the existing flupyrazone as an insecticide component, the present invention provides an insecticidal composition containing flupyrazone and its use. The insecticidal composition contains flupyrazone, high-efficiency flucythrin, basil extract, sage extract, oregano extract and other components. The components are used in a certain proportion, and different mechanisms of action act synergistically. It has excellent insecticidal effects and is effective against both piercing-sucking mouthparts pests in the growth period and adults. It has good rapidity and reduces the medication cycle; it solves the problems of resistance to flupyrazone and poor imitation, and can be used throughout the life cycle of crops; it is environmentally friendly, safe for crops, reduces the content of flupyrazone, and reduces environmental pollution. Its specific technical scheme is as follows:

[0010] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 5% to 8% of flupyrazone, 2% to 5% of high-efficiency flucythrin, 2% to 5% of basil extract, 2% to 5% of sage extract, 2% to 5% of oregano extract, 1% to 3% of alkylphenol polyoxyethylene ether, 0.5% to 2% of sodium lignin sulfonate, 0.3% to 0.5% of xanthan gum, 0.5% to 3% of propylene glycol, 0.05% to 0.1% of sodium benzoate, 0.1% to 0.3% of organosilicon defoamer, and the balance is deionized water; the basil extract is an extract obtained by fermenting basil with Paenibacillus polymyxa and Lactobacillus reuteri; the sage extract is an extract obtained by fermenting sage with Streptococcus faecalis and Paenibacillus polymyxa; and the oregano extract is an extract obtained by fermenting oregano with Streptococcus thermophilus and Lactobacillus crispatus.

[0011] In the above insecticide composition, the preparation method of the basil extract comprises the following steps: taking the whole basil plant and crushing it, adding water 12 to 15 times the mass of the basil, boiling it for 20 to 30 minutes, and cooling it to obtain a base liquid; adding a fermentation aid to the base liquid, and then adding 1% to 3% of the mass of basil Bacillus polymyxa, anaerobically fermenting it for 12 hours to 15 hours at 25°C to 37°C and pH 6.5 to 7.5, and then adding 1% to 3% of the mass of basil Lactobacillus reuteri, anaerobically fermenting it for 12 hours to 15 hours at 35°C to 38°C and pH 5.5 to 6.5, filtering it with a sieve, taking the filtrate, concentrating it to a paste, sterilizing it, and obtaining the basil extract.

[0012] In the preparation method of the basil extract, the bacterial activity of the polymyxa bacillus is 5 billion CFU / g to 10 billion CFU / g; the bacterial activity of the Lactobacillus reuteri is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, dipotassium hydrogen phosphate, magnesium sulfate, and ferrous sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid, and ferrous sulfate is 0.005% to 0.01% of the mass of the base liquid.

[0013] In the above-mentioned insecticide composition, the preparation method of the sage extract comprises the following steps: taking the whole plant of sage and crushing it, adding water 12 to 15 times the mass of the sage, boiling it for 20 to 30 minutes, and cooling it to obtain a base liquid; adding a fermentation aid to the base liquid, and then adding 1% to 3% of fecal streptococci by mass of the sage, aerobically fermenting it for 15 hours to 20 hours at 35°C to 38°C and pH 7.0 to 7.4, and then adding 1% to 3% of polymyxa bacillus by mass of the sage, anaerobically fermenting it for 12 hours to 15 hours at 25°C to 37°C and pH 6.5 to 7.5, filtering it with a sieve, taking the filtrate, concentrating it to a paste, sterilizing it, and obtaining the sage extract.

[0014] In the preparation method of the sage extract, the bacterial activity of the faecal streptococcus is 10 billion CFU / g to 20 billion CFU / g; the bacterial activity of the polymyxa bacillus is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, sodium chloride, dipotassium hydrogen phosphate, and magnesium sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, sodium chloride is 0.05% to 0.1% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, and magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid.

[0015] In the above insecticide composition, the preparation method of the oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 12 to 15 times the mass of the oregano, boiling it for 20 to 30 minutes, cooling it, and obtaining a base liquid; adding a fermentation aid to the base liquid, and then adding thermophilic streptococci 1% to 3% by mass of the oregano, and anaerobically fermenting it for 15 to 20 hours at 40 to 45° C. and pH 5.5 to 6.5, and then adding Lactobacillus crispatus 1% to 3% by mass of the oregano, and anaerobically fermenting it for 10 to 15 hours at 35 to 38° C. and pH 5.5 to 6.5, filtering it with a sieve, obtaining the filtrate, concentrating it to a paste, and sterilizing it to obtain the oregano extract.

[0016] In the preparation method of the oregano extract, the bacterial activity of the thermophilic streptococcus is 5 billion CFU / g to 10 billion CFU / g; the bacterial activity of the crispatus lactobacillus is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, dipotassium hydrogen phosphate, magnesium sulfate, and zinc sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid, and zinc sulfate is 0.005% to 0.01% of the mass of the base liquid.

[0017] In the above technical solution, the mesh size of the sieve is 200-250 meshes; the sterilization temperature is above 120° C.; and the water content of the paste is 50%.

[0018] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0019] The above-mentioned insecticidal composition containing flupyrazone is used for preventing and controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0020] The insecticidal composition containing flupyrazone and its use provided by the present invention have the following beneficial effects compared with the prior art:

[0021] 1. The insecticide composition of the present invention contains flupyrazone, high-efficiency cypermethrin, basil extract, sage extract, oregano extract and other ingredients; when used in a certain proportion, it has excellent insecticidal effect, can highly selectively control major piercing-sucking mouthparts pests, is effective for adults in the growth period, effectively controls hidden pests, has good rapid effect, and reduces the medication cycle; it solves the problem of resistance to flupyrazone and the problem of poor imitation, and can be used throughout the life cycle of crops. It is environmentally friendly, safe for crops, reduces the content of flupyrazone, and reduces environmental pollution.

[0022] 2. Flupyralidone is mainly used to control piercing-sucking mouthparts pests. It has good control effects on aphids, whiteflies, whiteflies, thrips, psyllids, etc., especially whiteflies and whiteflies. High-efficiency flucythrinate, also known as trihalocyhalothrin and kungfu cypermethrin, is a broad-spectrum insecticide that has contact and stomach poison effects on a variety of pests and can effectively control aphids and other pests. After the two are compounded, the insecticide spectrum is further expanded, and multiple pests can be controlled at the same time, improving the control effect. Flupyralidone acts on the central nervous system of pests, making nerve cells in an excited state, causing permanent opening of postsynaptic receptors, causing nervous system disorders, and then causing them to collapse, with good rapid effect. High-efficiency flucythrinate is a neurotoxin that can quickly exert contact killing effects after contacting pests, causing the pests to die of poisoning. The combination of the two can quickly knock down pests in a short period of time, enhancing the rapid effect of the agent.

[0023] These pests reproduce quickly and are prone to developing resistance. Flupyralidone has no cross-resistance with other insecticides, while the mechanism of action of high-efficiency flucythrinate is different from that of flupyralidone. After the two are combined, the number of action sites increases, which can delay the development of pest resistance and extend the service life of the agent. Flupyralidone has systemic, contact, stomach poison and osmotic effects, and can control pests from inside and outside the plant; high-efficiency flucythrinate mainly kills insects through contact and stomach poison, and has strong permeability. The combination of the two can work simultaneously when the pest contacts the agent and feeds on the plant, thereby improving the insecticidal effect. Flupyralidone acts on the central nervous system of insects and interferes with the transmission of nerve signals in pests; high-efficiency flucythrinate destroys the nervous system by interacting with the sodium channels of pests. The two agents affect the pest nervous system at different action sites, and the synergistic effect makes the dysfunction of the pest nervous system more serious, thereby enhancing the insecticidal effect.

[0024] 3. The basil extract of the present invention is an extract obtained by fermenting basil with Bacillus polymyxa and Lactobacillus reuteri. Basil contains a variety of biologically active ingredients, which can produce some substances with pungent odors and insecticidal ingredients after fermentation, which have the effect of repelling and preventing pests; at the same time, the fermentation products can interfere with the nervous system and physiological metabolism of pests, affecting their feeding, growth and reproduction.

[0025] Fourth, the sage extract of the present invention is an extract obtained by fermenting sage with Streptococcus faecalis and Paenibacillus polymyxa. The fermented and concentrated product has certain effects of killing, repelling and inhibiting growth and development of pests. The active ingredients in the fermented sage extract can destroy the digestive system and nervous system of pests and interfere with their endocrine system, thereby affecting the survival and reproduction of pests.

[0026] 5. The oregano extract of the present invention is an extract obtained by fermenting oregano with thermophilic streptococcus and crispatus lactobacillus. It has the effects of repelling, inhibiting growth and killing pests. The fermented oregano extract may contain active ingredients such as antibacterial and antioxidant, which can interfere with the physiological metabolism of pests and affect their survival ability.

[0027] 6. Flupyralidone, basil extract, sage extract, and oregano extract work together to target a variety of pests and improve the control effect on aphids, whiteflies, whiteflies, leafhoppers, thrips, and psyllids. Compared with using one ingredient alone, the compound can improve the intensity of the poisoning and repelling effects on pests. The combination of multiple ingredients with different mechanisms of action can reduce the risk of pests developing resistance to a single ingredient.

[0028] The different mechanisms of action of the four ingredients complement each other, making it difficult for pests to adapt, thereby improving the insecticidal effect. Certain ingredients in the extract can enhance the toxicity of flupyrazone to pests, affect the metabolism of flupyrazone by pests, and increase its toxicity in the pests. The combined use of the four ingredients can effectively reduce the dosage of flupyrazone and effectively reduce the adverse effects of flupyrazone on bees and the environment. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0030] Alkylphenol polyoxyethylene ether: NP-10; Propylene glycol: 1,3-propylene glycol; Silicone defoamer: KS-66.

[0031] Example 1

[0032] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 5% of flupyrazone, 2% of high-efficiency flucythrin, 2% of basil extract, 2% of sage extract, 2% of oregano extract, 1% of alkylphenol polyoxyethylene ether, 0.5% of sodium lignin sulfonate, 0.3% of xanthan gum, 0.5% of propylene glycol, 0.05% of sodium benzoate, 0.1% of organosilicon defoamer, and the balance is deionized water.

[0033] The preparation method of basil extract comprises the following steps: taking the whole basil plant for crushing, adding water 12 times the mass of basil, boiling for 20 minutes, cooling, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2% sucrose by mass of the base liquid, 2% peptone by mass of the base liquid, 0.2% dipotassium hydrogen phosphate by mass of the base liquid, 0.1% magnesium sulfate by mass of the base liquid, and 0.005% ferrous sulfate by mass of the base liquid; then adding 5 billion CFU / g of polymyxa bacillus by mass of 1% of the mass of basil, anaerobically fermenting for 12 hours at 25°C and pH 6.5, then adding 5 billion CFU / g of Lactobacillus reuteri by mass of 1% of the mass of basil, anaerobically fermenting for 12 hours at 35°C and pH 5.5, filtering through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, and sterilizing at 120°C to obtain the basil extract.

[0034] The preparation method of sage extract includes the following steps: taking the whole plant of sage and crushing it, adding water 12 times the mass of sage, boiling it for 20 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2% sucrose by mass of the base liquid, 2% peptone by mass of the base liquid, 0.05% sodium chloride by mass of the base liquid, 0.2% dipotassium hydrogen phosphate by mass of the base liquid, and 0.1% magnesium sulfate by mass of the base liquid; then adding 10 billion CFU / g of fecal streptococci by mass of 1% of the mass of sage, aerobically fermenting it for 15 hours at 35°C and pH7.0, then adding 5 billion CFU / g of polymyxa bacillus by mass of 1% of the mass of sage, anaerobically fermenting it for 12 hours at 25°C and pH6.5, filtering it with a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 120°C, and obtaining the sage extract.

[0035] The preparation method of oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 12 times the mass of the oregano, boiling it for 20 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2% sucrose by mass of the base liquid, 2% peptone by mass of the base liquid, 0.2% dipotassium hydrogen phosphate by mass of the base liquid, 0.1% magnesium sulfate by mass of the base liquid, and 0.005% zinc sulfate by mass of the base liquid; then adding 5 billion CFU / g of thermophilic streptococcus by mass of 1% of the oregano mass, anaerobic fermentation for 15 hours at 40°C and pH 5.5, then adding 5 billion CFU / g of crisp lactobacillus by mass of 1% of the oregano mass, anaerobic fermentation for 10 hours at 35°C and pH 5.5, filtering it through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 120°C, and obtaining the oregano extract.

[0036] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0037] The insecticidal composition containing flupyrazone of this embodiment is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0038] Example 2

[0039] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 6% of flupyrazone, 2.5% of high-efficiency cypermethrin, 2.5% of basil extract, 2.5% of sage extract, 3% of oregano extract, 1.5% of alkylphenol polyoxyethylene ether, 1% of sodium lignin sulfonate, 0.4% of xanthan gum, 0.6% of propylene glycol, 0.06% of sodium benzoate, 0.15% of organosilicon defoamer, and the balance is deionized water.

[0040] The preparation method of basil extract comprises the following steps: taking the whole basil plant for crushing, adding water 13 times the mass of basil, boiling for 20 minutes, cooling, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2.5% sucrose by mass of the base liquid, 2.5% peptone by mass of the base liquid, 0.3% dipotassium hydrogen phosphate by mass of the base liquid, 0.15% magnesium sulfate by mass of the base liquid, and 0.006% ferrous sulfate by mass of the base liquid; then adding 1.5% of the mass of basil and 5 billion CFU / g of polymyxa bacillus, anaerobically fermenting for 13 hours at 28°C and pH 6.8, then adding 1.5% of the mass of basil and 5 billion CFU / g of Lactobacillus reuteri, anaerobically fermenting for 13 hours at 36°C and pH 5.8, filtering through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, and sterilizing at 123°C to obtain the basil extract.

[0041] The preparation method of sage extract includes the following steps: taking the whole plant of sage and crushing it, adding water 13 times the mass of sage, boiling it for 20 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2.5% sucrose by mass of the base liquid, 2.5% peptone by mass of the base liquid, 0.06% sodium chloride by mass of the base liquid, 0.3% dipotassium hydrogen phosphate by mass of the base liquid, and 0.15% magnesium sulfate by mass of the base liquid; then adding 10 billion CFU / g of fecal streptococci by mass of 1.5% of the mass of sage, aerobically fermenting it for 16 hours at 36°C and pH7.1, then adding 5 billion CFU / g of polymyxa bacillus by mass of 1.5% of the mass of sage, anaerobically fermenting it for 13 hours at 28°C and pH6.8, filtering it with a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 123°C, and obtaining the sage extract.

[0042] The preparation method of oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 13 times the mass of the oregano, boiling it for 20 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 2.5% of sucrose by mass of the base liquid, 2.5% of peptone by mass of the base liquid, 0.25% of dipotassium hydrogen phosphate by mass of the base liquid, 0.15% of magnesium sulfate by mass of the base liquid, and 0.008% of zinc sulfate by mass of the base liquid; then adding 5 billion CFU / g of thermophilic streptococcus by mass of 1.5% of the mass of the oregano, anaerobic fermentation for 16 hours at 42° C. and pH 5.8, then adding 5 billion CFU / g of crisp lactobacillus by mass of 1.5% of the mass of the oregano, anaerobic fermentation for 11 hours at 36° C. and pH 5.8, filtering it through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 123° C., and obtaining the oregano extract.

[0043] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0044] The insecticidal composition containing flupyrazone of this embodiment is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0045] Example 3

[0046] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 6.5% of flupyrazone, 3.5% of high-efficiency cypermethrin, 3% of basil extract, 3% of sage extract, 3.5% of oregano extract, 2% of alkylphenol polyoxyethylene ether, 1.2% of sodium lignin sulfonate, 0.4% of xanthan gum, 2% of propylene glycol, 0.08% of sodium benzoate, 0.2% of organosilicon defoamer, and the balance is deionized water.

[0047] The preparation method of basil extract comprises the following steps: taking the whole basil plant for crushing, adding water 13 times the mass of basil, boiling for 25 minutes, cooling, and obtaining a base liquid; adding fermentation aids to the base liquid, including 3% sucrose by mass of the base liquid, 3% peptone by mass of the base liquid, 0.3% dipotassium hydrogen phosphate by mass of the base liquid, 0.2% magnesium sulfate by mass of the base liquid, and 0.008% ferrous sulfate by mass of the base liquid; then adding 10 billion CFU / g of polymyxa bacillus by mass of 2% of the mass of basil, anaerobically fermenting for 14 hours at 32° C. and pH 7.0, then adding 10 billion CFU / g of Lactobacillus reuteri by mass of 2% of the mass of basil, anaerobically fermenting for 14 hours at 37° C. and pH 6.0, filtering through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 60%, and sterilizing at 121° C. to obtain the basil extract.

[0048] The preparation method of sage extract includes the following steps: taking the whole plant of sage and crushing it, adding water 13 times the mass of sage, boiling it for 25 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 3% sucrose by mass of the base liquid, 3% peptone by mass of the base liquid, 0.08% sodium chloride by mass of the base liquid, 0.3% dipotassium hydrogen phosphate by mass of the base liquid, and 0.2% magnesium sulfate by mass of the base liquid; then adding 20 billion CFU / g of fecal streptococci by mass of 2% of the mass of sage, aerobically fermenting it for 18 hours at 37°C and pH7.2, then adding 10 billion CFU / g of polymyxa bacillus by mass of 2% of the mass of sage, anaerobically fermenting it for 14 hours at 32°C and pH7.0, filtering it with a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 60%, sterilizing it at 121°C, and obtaining the sage extract.

[0049] The preparation method of oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 13 times the mass of the oregano, boiling it for 25 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 3% sucrose by mass of the base liquid, 3% peptone by mass of the base liquid, 0.3% dipotassium hydrogen phosphate by mass of the base liquid, 0.2% magnesium sulfate by mass of the base liquid, and 0.008% zinc sulfate by mass of the base liquid; then adding 10 billion CFU / g of thermophilic streptococcus by mass of 2% of the oregano, anaerobically fermenting it for 18 hours at 42° C. and pH 6.0, then adding 10 billion CFU / g of crisp lactobacillus by mass of 2% of the oregano, anaerobically fermenting it for 14 hours at 37° C. and pH 6.0, filtering it through a 200-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 60%, sterilizing it at 121° C., and obtaining the oregano extract.

[0050] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0051] The insecticidal composition containing flupyrazone of this embodiment is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0052] Example 4

[0053] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 7% flupyrazone, 4% high-efficiency cypermethrin, 4% basil extract, 4% sage extract, 3.5% oregano extract, 2.5% alkylphenol polyoxyethylene ether, 1.5% sodium lignin sulfonate, 0.4% xanthan gum, 2.5% propylene glycol, 0.08% sodium benzoate, 0.2% organosilicon defoamer, and the balance is deionized water.

[0054] The preparation method of basil extract comprises the following steps: taking the whole basil plant for crushing, adding water 14 times the mass of basil, boiling for 30 minutes, cooling, and obtaining a base liquid; adding fermentation aids to the base liquid, including 4% sucrose by mass of the base liquid, 4% peptone by mass of the base liquid, 0.4% dipotassium hydrogen phosphate by mass of the base liquid, 0.25% magnesium sulfate by mass of the base liquid, and 0.008% ferrous sulfate by mass of the base liquid; then adding 10 billion CFU / g of polymyxa bacillus by mass of 2.5% of the mass of basil, anaerobically fermenting for 14 hours at 35°C and pH 7.2, then adding 10 billion CFU / g of Lactobacillus reuteri by mass of 2.5% of the mass of basil, anaerobically fermenting for 14 hours at 37°C and pH 6.2, filtering with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, and sterilizing at 124°C to obtain the basil extract.

[0055] The preparation method of sage extract includes the following steps: taking the whole plant of sage and crushing it, adding water 14 times the mass of sage, boiling it for 30 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 4% sucrose by mass of the base liquid, 4% peptone by mass of the base liquid, 0.08% sodium chloride by mass of the base liquid, 0.4% dipotassium hydrogen phosphate by mass of the base liquid, and 0.25% magnesium sulfate by mass of the base liquid; then adding 20 billion CFU / g of fecal streptococci by mass of 2.5% of the mass of sage, aerobically fermenting it for 18 hours at 37°C and pH7.3, then adding 10 billion CFU / g of polymyxa bacillus by mass of 2.5% of the mass of sage, anaerobically fermenting it for 14 hours at 35°C and pH7.3, filtering it with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 124°C, and obtaining the sage extract.

[0056] The preparation method of oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 14 times the mass of the oregano, boiling it for 30 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 4% sucrose by mass of the base liquid, 4% peptone by mass of the base liquid, 0.4% dipotassium hydrogen phosphate by mass of the base liquid, 0.25% magnesium sulfate by mass of the base liquid, and 0.008% zinc sulfate by mass of the base liquid; then adding 10 billion CFU / g of thermophilic streptococcus by mass of 2.5% of the mass of the oregano, anaerobically fermenting it for 18 hours at 43° C. and pH 6.2, then adding 10 billion CFU / g of crisp lactobacillus by mass of 2.5% of the mass of the oregano, anaerobically fermenting it for 14 hours at 37° C. and pH 6.2, filtering it with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 124° C., and obtaining the oregano extract.

[0057] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0058] The insecticidal composition containing flupyrazone of this embodiment is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0059] Example 5

[0060] The invention discloses an insecticide composition containing flupyrazone, which comprises the following raw materials in percentage by mass: 8% of flupyrazone, 5% of high-efficiency flucythrin, 5% of basil extract, 5% of sage extract, 5% of oregano extract, 3% of alkylphenol polyoxyethylene ether, 2% of sodium lignin sulfonate, 0.5% of xanthan gum, 3% of propylene glycol, 0.1% of sodium benzoate, 0.3% of organosilicon defoamer, and the balance is deionized water.

[0061] The preparation method of basil extract comprises the following steps: taking the whole basil plant for crushing, adding water 15 times the mass of basil, boiling for 30 minutes, cooling, and obtaining a base liquid; adding fermentation aids to the base liquid, including 5% sucrose by mass of the base liquid, 5% peptone by mass of the base liquid, 0.5% dipotassium hydrogen phosphate by mass of the base liquid, 0.3% magnesium sulfate by mass of the base liquid, and 0.01% ferrous sulfate by mass of the base liquid; then adding 10 billion CFU / g of polymyxa bacillus by mass of 3% of the mass of basil, anaerobic fermentation for 15 hours at 37°C and pH7.5, then adding 10 billion CFU / g of Lactobacillus reuteri by mass of 3% of the mass of basil, anaerobic fermentation for 15 hours at 38°C and pH6.5, filtering with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, and sterilizing at 125°C to obtain the basil extract.

[0062] The preparation method of sage extract includes the following steps: taking the whole plant of sage and crushing it, adding water 15 times the mass of sage, boiling it for 30 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 5% sucrose by mass of the base liquid, 5% peptone by mass of the base liquid, 0.1% sodium chloride by mass of the base liquid, 0.5% dipotassium hydrogen phosphate by mass of the base liquid, and 0.3% magnesium sulfate by mass of the base liquid; then adding 20 billion CFU / g of fecal streptococci by mass of 3% of the mass of sage, aerobically fermenting it for 20 hours at 38°C and pH7.4, then adding 10 billion CFU / g of polymyxa bacillus by mass of 3% of the mass of sage, anaerobically fermenting it for 15 hours at 37°C and pH7.5, filtering it with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 125°C, and obtaining the sage extract.

[0063] The preparation method of oregano extract comprises the following steps: taking the whole oregano plant and crushing it, adding water 15 times the mass of the oregano, boiling it for 30 minutes, cooling it, and obtaining a base liquid; adding fermentation aids to the base liquid, including 5% sucrose by mass of the base liquid, 5% peptone by mass of the base liquid, 0.5% dipotassium hydrogen phosphate by mass of the base liquid, 0.3% magnesium sulfate by mass of the base liquid, and 0.01% zinc sulfate by mass of the base liquid; then adding 10 billion CFU / g of thermophilic streptococcus by mass of 3% of the oregano, anaerobically fermenting it for 20 hours at 45°C and pH 6.5, then adding 10 billion CFU / g of crisp lactobacillus by mass of 3% of the oregano, anaerobically fermenting it for 15 hours at 38°C and pH 6.5, filtering it with a 250-mesh sieve, taking the filtrate, concentrating it to a paste with a water content of 50%, sterilizing it at 125°C, and obtaining the oregano extract.

[0064] The preparation method of the above-mentioned insecticidal composition containing flupyrazone comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and silicone defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrinate, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticidal composition.

[0065] The insecticidal composition containing flupyrazone of this embodiment is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

[0066] Comparative Example 1

[0067] No basil extract was added to the insecticide composition; other parameters and methods were the same as in Example 1.

[0068] Comparative Example 2

[0069] No sage extract was added to the insecticide composition; other parameters and methods were the same as in Example 1.

[0070] Comparative Example 3

[0071] No oregano extract was added to the insecticide composition; other parameters and methods were the same as in Example 1.

[0072] Comparative Example 4

[0073] The insecticidal composition does not contain basil extract, sage extract and oregano extract; other parameters and methods are the same as those in Example 1.

[0074] Comparative Example 5

[0075] The basil extract was not fermented with Paenibacillus polymyxa; other parameters and methods were the same as those in Example 1.

[0076] Comparative Example 6

[0077] The sage extract was not fermented with Streptococcus faecalis; other parameters and methods were the same as in Example 1.

[0078] Comparative Example 7

[0079] The oregano extract was not fermented with Lactobacillus crispatus; other parameters and methods were the same as in Example 1.

[0080] Comparative Example 8

[0081] In the insecticide composition, the basil extract, the sage extract, and the oregano extract are all extracted by water extraction, that is, after boiling in water for 25 minutes, filtered through a 200-mesh sieve, the filtrate is concentrated to a paste with a water content of 60%, and sterilized at 121° C. to obtain the extract. Other parameters and methods are the same as in Example 1.

[0082] The insecticidal compositions of the above-mentioned embodiments and comparative examples were subjected to insecticidal detection tests.

[0083] 1. Spray method

[0084] Several plant leaves with healthy adults and nymphs of aphids that have not been exposed to pesticides were collected and divided into 14 groups on average, one of which was a blank control group. Each group was evenly placed in 5 insect cages (200 aphids in each insect cage), and the insecticide composition was evenly sprayed on the plant leaves with a sprayer. The blank control group was sprayed with water; observation was carried out in a constant temperature and humidity environment (temperature 25°C, relative humidity 65%), and the aphid mortality rate was recorded within 24 hours. The test results are shown in Table 1.

[0085] The determination methods for whitefly, whitefly, leafhopper and thrips were the same as above. The test results are shown in Table 1.

[0086] 2. Dipping method

[0087] Several plant leaves with healthy adults and nymphs of psyllids that have not been exposed to insecticides were collected and divided into 14 groups on average, one of which was a blank control group. Each group was evenly placed in 5 insect cages (200 in each insect cage). The leaves with psyllids were immersed in the insecticide solution containing the insecticide composition, and taken out after immersion for 10 seconds. The excess solution was absorbed with filter paper, and then the treated leaves were placed in the insect cage. The observation was carried out in a constant temperature and humidity environment (temperature 25°C, relative humidity 65%), and the mortality rate of psyllids was recorded for 24 hours. The test results are shown in Table 1.

[0088] Table 1 Insecticidal effect test results

[0089]

[0090]

[0091] From the above results, it can be seen that the insecticidal compositions of Examples 1 to 5 have good killing effects on aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips, and the 3h pest mortality rate can reach more than 70%, and the 24h pest mortality rate can reach more than 90%, and the killing effect on psyllids is particularly significant, with a 24h mortality rate of more than 98%; from the results of Comparative Examples 1 to 3, it can be seen that when basil extract, sage extract or oregano extract are not added to the insecticidal composition, the insecticidal effect of the composition is reduced, indicating that basil extract, sage extract and oregano extract all have a promoting effect on the prevention and control of pests by flupyrazone; from the results of Comparative Example 4, it can be seen that when basil extract, sage extract or oregano extract are not added to the insecticidal composition at the same time, the insecticidal effect of the composition is significantly reduced, and the 24h mortality rate is less than 81%; from the results of comparative example 5, it can be seen that the basil extract is not fermented with Bacillus polymyxa, the extract product components are different, the insect control promoting effect is different, resulting in a decrease in the insecticidal effect of the composition; from the results of comparative example 6, it can be seen that the sage extract is not fermented with Streptococcus faecalis, the extract product components are different, the insect control promoting effect is different, resulting in a decrease in the insecticidal effect of the composition; from the results of comparative example 7, it can be seen that the oregano extract is not fermented with Lactobacillus crispatus, the extract product components are different, the insect control promoting effect is different, resulting in a decrease in the insecticidal effect of the composition; from the results of comparative example 8, it can be seen that in the insecticidal composition, the basil extract, the sage extract, and the oregano extract are all extracted by water extraction, the extract product components are different, the insecticidal effect of the composition is significantly reduced, and the 24h mortality rate is less than 84%.

[0092] In addition, conventional insecticides contain 15% to 17% of fluoropyrazone, and the insecticide mortality rate after 24 hours is more than 80%. The amount of fluoropyrazone added in each embodiment of the present invention is only 5% to 8%. After adding basil extract, sage extract and oregano extract, the combination with fluoropyrazone has a good synergistic effect. Compared with conventional insecticides containing 15% to 17% of fluoropyrazone, each embodiment has a better insecticidal effect, and the insecticide mortality rate after 24 hours can reach more than 90%, which can well reduce the dosage of fluoropyrazone and effectively reduce the adverse effects of fluoropyrazone on bees and the environment.

Claims

1. An insecticidal composition containing flupyrazone, characterized in that: The insecticide composition comprises the following raw materials in percentage by mass: 5% to 8% of flupyralidone, 2% to 5% of high-efficiency flucythrin, 2% to 5% of basil extract, 2% to 5% of sage extract, 2% to 5% of oregano extract, 1% to 3% of alkylphenol polyoxyethylene ether, 0.5% to 2% of sodium lignin sulfonate, 0.3% to 0.5% of xanthan gum, 0.5% to 3% of propylene glycol, 0.05% to 0.1% of sodium benzoate, 0.1% to 0.3% of organosilicon defoamer, and the balance is deionized water; The basil extract is an extract obtained by fermenting basil with Paenibacillus polymyxa and Lactobacillus reuteri; The sage extract is an extract obtained by fermenting sage with Streptococcus faecalis and Paenibacillus polymyxa; The oregano extract is an extract obtained by fermenting oregano with thermophilic streptococcus and crispatus lactobacillus.

2. The insecticidal composition containing flupyrazone according to claim 1, characterized in that: The preparation method of the basil extract comprises the following steps: taking the whole basil and crushing it, adding water with a mass of 12 to 15 times the mass of the basil, boiling it for 20 to 30 minutes, and cooling it to obtain a base liquid; adding a fermentation aid to the base liquid, and then adding 1% to 3% of the mass of basil Paenibacillus polymyxa, and performing anaerobically fermentation for 12 to 15 hours at 25 to 37 degrees Celsius and pH 6.5 to 7.5, and then adding 1% to 3% of the mass of basil Lactobacillus reuteri, and performing anaerobically fermentation for 12 to 15 hours at 35 to 38 degrees Celsius and pH 5.5 to 6.5, filtering with a sieve, taking the filtrate, concentrating it to a paste, and sterilizing it to obtain the basil extract.

3. The insecticidal composition containing flupyrazone according to claim 2, characterized in that: The bacterial activity of the polymyxa bacillus is 5 billion CFU / g to 10 billion CFU / g; the bacterial activity of the Lactobacillus reuteri is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, dipotassium hydrogen phosphate, magnesium sulfate, and ferrous sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid, and ferrous sulfate is 0.005% to 0.01% of the mass of the base liquid.

4. The insecticidal composition containing flupyrazone according to claim 1, characterized in that: The preparation method of the sage extract comprises the following steps: taking a whole plant of sage and crushing it, adding water 12 to 15 times the mass of the sage, boiling it for 20 to 30 minutes, and cooling it to obtain a base liquid; adding a fermentation aid to the base liquid, and then adding 1% to 3% of fecal streptococci by mass of the sage, aerobically fermenting it for 15 to 20 hours at 35 to 38 degrees Celsius and pH 7.0 to 7.4, and then adding 1% to 3% of polymyxa bacillus by mass of the sage, and anaerobically fermenting it for 12 to 15 hours at 25 to 37 degrees Celsius and pH 6.5 to 7.5, filtering it with a sieve, taking the filtrate, concentrating it to a paste, and sterilizing it to obtain the sage extract.

5. The insecticidal composition containing flupyrazone according to claim 4, characterized in that: The bacterial activity of the fecal streptococcus is 10 billion CFU / g to 20 billion CFU / g; the bacterial activity of the polymyxa bacillus is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, sodium chloride, dipotassium hydrogen phosphate, and magnesium sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, sodium chloride is 0.05% to 0.1% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, and magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid.

6. The insecticidal composition containing flupyrazone according to claim 1, characterized in that: The preparation method of the oregano extract comprises the following steps: taking a whole oregano plant and crushing it, adding water 12 to 15 times the mass of the oregano, boiling it for 20 to 30 minutes, and cooling it to obtain a base liquid; adding a fermentation aid to the base liquid, and then adding thermophilic streptococci 1% to 3% by mass of the oregano, and carrying out anaerobically fermenting it for 15 to 20 hours at 40 to 45° C. and pH 5.5 to 6.5, and then adding Lactobacillus crispatus 1% to 3% by mass of the oregano, and carrying out anaerobically fermenting it for 10 to 15 hours at 35 to 38° C. and pH 5.5 to 6.5, filtering it with a sieve, taking the filtrate, concentrating it to a paste, and sterilizing it to obtain the oregano extract.

7. The insecticidal composition containing flupyrazone according to claim 6, characterized in that: The bacterial activity of the thermophilic streptococcus is 5 billion CFU / g to 10 billion CFU / g; the bacterial activity of the crisp lactobacillus is 5 billion CFU / g to 10 billion CFU / g; the fermentation aids include sucrose, peptone, dipotassium hydrogen phosphate, magnesium sulfate, and zinc sulfate, and the added amounts are: sucrose is 2% to 5% of the mass of the base liquid, peptone is 2% to 5% of the mass of the base liquid, dipotassium hydrogen phosphate is 0.2% to 0.5% of the mass of the base liquid, magnesium sulfate is 0.1% to 0.3% of the mass of the base liquid, and zinc sulfate is 0.005% to 0.01% of the mass of the base liquid.

8. An insecticidal composition containing flupyrazone according to claim 2, 4 or 6, characterized in that: The mesh number of the sieve is 200-250 meshes; the sterilization temperature is above 120° C.; and the water content of the paste is 50%.

9. The insecticidal composition containing flupyrazone according to claim 1, characterized in that: The preparation method of the insecticide composition comprises the following steps: weighing raw materials according to mass percentage, dissolving xanthan gum in deionized water, then adding alkylphenol polyoxyethylene ether, sodium lignin sulfonate, propylene glycol, and an organosilicon defoamer to mix evenly, then adding flupyrazone, high-efficiency flucythrin, basil extract, sage extract, and oregano extract to mix evenly, and finally adding sodium benzoate to mix evenly to obtain the insecticide composition.

10. The use of an insecticidal composition containing flupyrazone according to claim 1, characterized in that: The insecticidal composition is used for controlling aphids, whiteflies, whiteflies, leafhoppers, psyllids and thrips.

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