Traditional Chinese medicine composition with anti-inflammatory and soothing effects and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition with anti-inflammatory and soothing effects. The traditional Chinese medicine composition is prepared from Chinese honeylocust fruits, ligustrum quihoui, folium artemisiae argyi and pitaya stems. The traditional Chinese medicine composition has exact cell inflammatory factor inhibition and soothing activity, and can be applied to various skin care products such as shampoo, facial cleanser and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a Chinese medicine composition with anti-inflammatory and soothing effects and a use thereof. Background Art

[0002] Dermatitis refers to inflammation of the skin. It is a general term for inflammatory skin diseases, not an independent disease, and is relatively common in clinical practice. The etiology is complex and may be related to contact with allergens, genetics, immunity, environmental factors, abnormal skin barrier function, etc. Its clinical manifestations are diverse. Patients may experience redness, swelling, dryness or itching of the skin, or blistering, exudation, scabs or desquamation of the skin.

[0003] Inflammation of the head and face is usually caused by folliculitis, seborrheic dermatitis, infection, etc. It is very necessary to take appropriate methods to treat it, but care should also be taken during the treatment. Patients should pay attention to maintaining a normal life and work and rest habits, and should also pay attention to quitting smoking and drinking, and not eating spicy or irritating foods, otherwise it will aggravate local infectious symptoms and affect the treatment effect. At the same time, they should also pay attention to personal hygiene.

[0004] Scalp folliculitis: Scalp folliculitis can cause follicular inflammatory papules on the patient's scalp. The patient may also have symptoms such as local itching, pain, increased dandruff, and greasy scalp. The treatment principle of scalp folliculitis is to kill bacteria and relieve itching. For scalp folliculitis caused by bacterial infection, patients can use antibacterial drugs such as mupirocin ointment and fusidic acid ointment; when there is a fungal infection, terbinafine cream, ketoconazole lotion, etc. can be used externally.

[0005] Seborrheic dermatitis: Seborrheic dermatitis is a chronic inflammatory skin disease and is also a common cause of scalp inflammation, which can cause symptoms such as erythematous papules, desquamation, and itching. Treatment can be topical selenium sulfide, salicylic acid lotion and other drugs, and in the case of fungal infection, oral itraconazole and other drugs can also be used.

[0006] Scalp infection: Scalp inflammation may also be caused by trauma. For scalp infections caused by bacterial infections, you can take antibiotics such as cefdinir dispersible tablets orally, and you can also apply ointments such as mupirocin cream topically. For fungal infections, you need to use antifungal drugs, such as terbinafine, itraconazole, etc.

[0007] Therefore, it can be seen that head and face inflammation is mainly treated with chemical antibacterial drugs, but long-term use of chemical antibacterial drugs will have significant side effects. Traditional Chinese medicine has a long history in anti-inflammatory and anti-infective diseases. Therefore, finding anti-inflammatory drugs from traditional Chinese medicine has important economic and social value.

[0008] Gleditsia sinensis Lam. is the fruit of the leguminous plant Gleditsia sinensis Lam. after aging or being damaged. Traditional Chinese medicine believes that it has the functions of clearing the orifices, removing phlegm, removing wind, and killing insects. It can be used to treat stroke, lockjaw, wind, wind chronicity, throat paralysis, phlegm and asthma, fullness and stagnation, obstruction of the body, carbuncle, scabies, ringworm, head sore and other diseases. Gleditsia is also a commonly used cleaning material. For example, it was used to wash clothes in ancient times; in modern times, many shampoos and other daily chemical products have added gleditsia.

[0009] Ligustrum quihoui Carr. is a small shrub of the genus Ligustrum in the family Oleaceae. Its young leaves are often used as Kuding tea in the southwest region. It has the effects of clearing away heat and detoxifying, and is also used to treat burns and trauma. However, it is rare to see Ligustrum quihoui Carr. used in skin care products.

[0010] Artemisia argyi Levl.et Van, also known as mugwort, is a common plant of the genus Artemisia in the Asteraceae family. It is a perennial herb or slightly semi-shrubby plant with a strong aroma. It has the effects of warming the yang, regulating qi and blood, dispelling cold and dampness, stopping bleeding and stabilizing pregnancy. It is often used for menstrual disorders, excessive menstrual bleeding, metrorrhagia, bleeding during pregnancy, cold pain in the abdomen, bacillary dysentery, impetigo, rhinitis and other symptoms of deficiency and cold; it has the effects of relieving cough, relieving asthma, removing phlegm, antibacterial, anti-allergic, analgesic, and hemostasis.

[0011] Pitaya Hylocereus undulatus Britt. is a perennial climbing fleshy shrub of the genus Hylocereus in the Cactaceae family. Its fruit is a common and delicious fruit. The stem of pitaya contains rich mucilage, polysaccharides and flavonoids, but the medicinal use of pitaya stem is not yet widespread.

[0012] Based on the above reasons, the present invention provides a traditional Chinese medicine composition with anti-inflammatory and soothing effects. The traditional Chinese medicine composition is composed of saponins, Ligustrum lucidum, wormwood and pitaya stem. The traditional Chinese medicine composition has good anti-inflammatory and soothing activity and can be used in various skin care products such as shampoo and facial cleanser. Summary of the invention

[0013] The main purpose of the present invention is to provide an anti-inflammatory and soothing Chinese medicine composition with definite efficacy, few side effects, and natural safety, so as to solve the problems of poor anti-inflammatory effect or easy allergy of common cleaning products in the prior art.

[0014] In order to achieve the above object, according to one aspect of the present invention, a Chinese medicine composition with anti-inflammatory and soothing effects is provided, characterized in that the Chinese medicine composition is composed of 20 parts of Gleditsia sinensis, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of Pitaya stems.

[0015] Furthermore, the traditional Chinese medicine composition further comprises one or more drugs and / or extracts for anti-inflammatory and antioxidant effects.

[0016] Furthermore, the anti-inflammatory drugs are aspirin, cefixime, roxithromycin tablets, and amoxicillin.

[0017] Furthermore, the antioxidant drug is vitamin C, vitamin E, resveratrol, quercetin, and dihydroquercetin.

[0018] According to another aspect of the present invention, there is provided a use of the above-mentioned Chinese medicine composition in terms of anti-inflammatory and soothing effects and its application in various skin care products such as shampoo and facial cleanser.

[0019] Beneficial effects of the present invention:

[0020] By applying the technical scheme of the present invention, the Chinese medicine composition of the present invention is composed of 20 parts of Gleditsia sinensis, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of Pitaya stems. The Chinese medicine composition has good anti-inflammatory and soothing activity, can significantly improve the level of cellular inflammatory factors, and well solves the problems of poor anti-inflammatory effect and easy allergic phenomenon of common washing and care products. DETAILED DESCRIPTION

[0021] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0022] The present invention is intended to encompass all alternatives, variations and equivalents that may be included in the present invention as defined by the claims. Those skilled in the art will recognize many methods and materials similar or equivalent to those described herein that can be used in the practice of the present invention. The present invention is in no way limited to the methods and materials described.

[0023] Unless otherwise specified or there is a clear conflict in context, the articles "a", "an", and "the" used in this invention are intended to include "at least one" or "one or more". Therefore, these articles used in this invention refer to one or more than one (i.e., at least one) object.

[0024] As described in the background technology section, existing common cleaning products have problems such as poor anti-inflammatory effect and easy allergic reaction. In order to solve the above problems, the present invention provides a Chinese medicine composition, which is composed of 20 parts of sapodilla, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of pitaya stems.

[0025] In this embodiment, the traditional Chinese medicine composition further comprises one or more drugs and / or extracts for anti-inflammatory and antioxidant effects.

[0026] Furthermore, the anti-inflammatory drugs are aspirin, cefixime, roxithromycin tablets, and amoxicillin.

[0027] Furthermore, the antioxidant drug is vitamin C, vitamin E, resveratrol, quercetin, and dihydroquercetin.

[0028] The present invention also provides a use of the above-mentioned traditional Chinese medicine composition in terms of anti-inflammatory and soothing effects and its application in various skin care products such as shampoo and facial cleanser.

[0029] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0030] The present invention is further described in detail below in conjunction with specific embodiments. These embodiments should not be construed as limiting the scope of protection claimed in this application.

[0031] Example

[0032] Example 1 Preparation of Chinese medicine composition 1-11

[0033] The Chinese medicine composition 1-11 of the present invention adopts a uniform design experimental scheme: referring to the literature (Liang Maoxin, Li Dongan. Improved application of uniform design in the selection of small Chinese medicine compound prescriptions [J]. World Science and Technology - Modernization of Traditional Chinese Medicine, 2006, 8 (02): 53-56.), the uniform design scheme U11 (11 5 )(As shown in Table 1), four Chinese medicinal materials in the prescription, namely, sapodilla, Ligustrum lucidum, wormwood leaf, and pitaya stem, were used as investigation factors. Each factor had 11 levels (i.e., 11 different doses with increasing gradient), and the investigation dose was between 0 and 50 g. The factors and levels were arranged according to the uniform design table, and the prescription design was carried out to construct a total of 11 groups of Chinese medicine compositions.

[0034] Table 1 Chinese medicine composition U11(11 5 )Uniform Design Table

[0035]

[0036]

[0037] Preparation of Chinese medicine composition 1-11 According to the amount of medicinal materials of composition 1-11 in Table 1, weigh each medicinal material according to the prescription of each group, crush it into coarse powder, and mix it evenly. According to the total amount of medicinal materials in each group, add 12 times the amount of purified water, soak for 1 hour, decoct for 1.5 hours, cool, filter, and collect the filtrate. Add 10 times the amount of purified water of the original medicinal material to the residue, decoct again for 1 hour, cool, filter, combine the filtrates of the two decoctions, and concentrate to 2 times the volume of the original medicinal material (volume / mass ratio), let it stand to 70°C, add 3 times the amount of 95% ethanol while hot, let it stand for 4 hours, take the supernatant, reduce pressure to recover ethanol and concentrate, and dry the concentrate at 60°C under reduced pressure to obtain the extract of Chinese medicine composition 1-11.

[0038] Example 2 Evaluation of the Cellular Anti-inflammatory Activity of Chinese Medicine Compositions 1-11

[0039] Raw264.7 cells in the logarithmic growth phase were taken and prepared into a single cell suspension using complete medium containing 10% FBS and 1% streptomycin. The concentration was adjusted to 10 5 / mL, inoculated in a 96-well plate, cultured for 18 hours, and after the cells adhered to the wall, 1g / mL of the extracts of the Chinese medicine composition 1-11 were added, and after pretreatment for 5 hours, 1.0μg / mL of lipopolysaccharide (LPS) was added. After incubation for 24 hours, the 96-well plate was placed on a centrifuge and centrifuged at 1000×g for 10 minutes. The supernatant was taken and the NO content was determined according to the instructions of the nitric oxide kit (Shanghai ELISA Biotechnology Co., Ltd.). The supernatant was taken separately and the contents of inflammatory factors IL-6, IL-1β, and TNF-α were determined according to the instructions of the enzyme-linked immunosorbent assay kit (Shanghai ELISA Biotechnology Co., Ltd.). The results are shown in Table 2.

[0040] Table 2 Effects of different compositions on NO and inflammatory factors in RAW264.7 cells induced by lipopolysaccharide (n=5)

[0041]

[0042]

[0043] From the data in Table 2, it can be seen that Chinese medicine composition 5 (20 parts of sapodilla, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of pitaya stems), Chinese medicine composition 8 (35 parts of sapodilla, 20 parts of Ligustrum lucidum, 5 parts of Artemisia argyi and 30 parts of pitaya stems) and Chinese medicine composition 10 (45 parts of sapodilla, 40 parts of Ligustrum lucidum, 35 parts of Artemisia argyi and 25 parts of pitaya stems) can better inhibit the release of cellular NO and inflammatory factors, and show good anti-inflammatory activity. In particular, Chinese medicine composition 5 (20 parts of sapodilla, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of pitaya stems) has the strongest anti-inflammatory activity.

[0044] Example 3 Evaluation of the anti-inflammatory activity of Chinese medicine composition 5 in animals

[0045] Fifty male ICR mice (provided by the Experimental Animal Center of Zunyi Medical University, Animal Production License No.: SCXK (Qian) 2019-0001) were randomly divided into 5 groups, 10 mice in each group, after adaptive feeding for 1 week, respectively: ① model group (gavage with 0.5% CMC-Na solution), ② positive control group (gavage with 0.2g / kg / d aspirin Bayer Pharmaceuticals and Healthcare Co., Ltd.), ③ high-dose group of Composition 5 (gavage with 1g / kg / d Composition 5 extract prepared by the method of Example 1, the same below), ④ medium-dose group of Composition 5 (gavage with 0.5g / kg / d Composition 5 extract), ⑤ low-dose group of Composition 5 (gavage with 0.25g / kg / d Composition 5 extract). The model group was gavaged with 0.5% CMC-Na solution, and the other groups were gavaged with equal volumes of corresponding drugs for five consecutive days. After 5 consecutive days of drug administration, xylene (Nanjing Chemical Reagent Co., Ltd.) was used to stimulate the mouse auricle to cause inflammation 1 hour after the last drug administration. The mice were killed 1 hour later, and both ears were removed along the auricle baseline. One ear piece was removed from the same part with a puncher and weighed with an electronic balance. The weight of the inflamed ear piece minus the weight of the control ear piece was the swelling degree, and the inhibition rate was calculated. The swelling degree is the weight difference of the bilateral ear pieces (mg): inhibition rate = (swelling degree of the model group - swelling degree of the drug administration group) / swelling degree of the model group × 100%.

[0046] The results are shown in Table 3. Compared with the model group, high, medium and low doses of Chinese medicine composition 5 can significantly inhibit the ear swelling of mice induced by xylene (P<0.05), and are dose-dependent, indicating that Chinese medicine composition 5 (composed of 20 parts of saponin, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of pitaya stem) has good anti-inflammatory activity.

[0047] Table 3 Effects of extracts from Chinese herbal composition 5 on xylene-induced ear swelling in mice

[0048] Group Number of animals Dose / g·kg-1 Swelling degree / mg Inhibition rate / % Model Group 10 - 9.36±2.14 - Aspirin group 10 0.2 2.68±0.96* 71.37 High dose group 10 1.0 2.57±0.88* 72.54 Medium dose group 10 0.5 3.91±1.01* 58.23 Low dose group 10 0.25 6.02±1.43* 35.68

[0049] Note: Compared with the model group, *p<0.05; the swelling degree is the difference in bilateral ear weight (mg);

[0050] Inhibition rate = (swelling degree of model group - swelling degree of drug administration group) / swelling degree of model group × 100%

[0051] Example 4 Preparation of Chinese medicine composition 5 shampoo

[0052] The shampoo formula comprises: 52.65% water, 16.0% cocamide propyl betaine, 8.0% lauryl glucoside, 10.0% extract of Chinese medicine composition 5 (prepared by the method of Example 1), 5.0% glycerol, 5.0% sodium laureth sulfate, 2.0% sodium C14-18 olefin sulfonate, 0.1% disodium EDTA, 0.15% citric acid, 0.5% phenoxyethanol, 0.5% sodium benzoate and 0.1% essence.

[0053] Preparation process: Add the prescribed amount of water, Chinese medicine composition 5 extract, and sodium laureth sulfate into the main pot, stir and heat to 85°C to fully dissolve them; then add cocamide propyl betaine, glycerin, sodium C14-18 olefin sulfonate, and disodium EDTA and continue to stir evenly; cool to 45°C and add the remaining components in turn, continue to stir slowly and evenly, and after passing the inspection, fill, package, inspect the finished products, and put them into storage.

[0054] Preliminary volunteer trials showed that the shampoo prepared with the extract of Chinese medicine composition 5 had no bad odor or irritation, and had obvious anti-inflammatory, soothing and anti-dandruff effects.

[0055] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

[0056] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, changes or deformations made by those skilled in the art based on the ideas of the present invention, the specific implementation methods and application scope of the present invention, all belong to the scope of protection of the present invention. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A Chinese medicine composition with anti-inflammatory and soothing effects, characterized in that: The traditional Chinese medicine composition is composed of 20 parts of sapodilla, 45 parts of Ligustrum lucidum, 15 parts of Artemisia argyi and 10 parts of pitaya stems.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is further used in combination with one or more anti-inflammatory and anti-oxidative drugs and / or extracts.

3. The Chinese medicine composition according to claim 2, characterized in that: The anti-inflammatory drugs are aspirin, cefixime, roxithromycin tablets and amoxicillin.

4. The Chinese medicine composition according to claim 2, characterized in that: The antioxidant drugs are vitamin C, vitamin E, resveratrol, quercetin and dihydroquercetin.

5. The anti-inflammatory and soothing effects of the traditional Chinese medicine composition according to claims 1-4 and its application in various skin care products such as shampoo and facial cleanser.

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