A composition, application and cosmetic for improving mitochondrial energy based on plant stem cells

Abstract

The present invention belongs to the field of daily chemicals, and discloses a mitochondrial energy improvement composition, application and cosmetic based on plant stem cells. The composition comprises the following components in parts by weight: 0.0005 - 1 part of sunflower bud extract; 0.0005 - 1 part of lemon balm extract; 0.0005 - 1 part of epigallocatechin gallate or epigallocatechin gallate. The composition of the present invention combines sunflower bud extract, lemon balm extract, epigallocatechin gallate or epigallocatechin gallate, and it is found that the combination of the three has obvious ability to improve cell mitochondrial dysfunction, enhance mitochondrial energy and increase the content of type I collagen, and can be used as a potential alternative for anti-wrinkle active ingredients in cosmetics.

Description

Technical Field

[0001] The invention relates to the field of daily chemicals, and in particular to a mitochondrial energy improving composition based on plant stem cells, its application and cosmetics. Background Art

[0002] Cellular energy ATP has been a hot topic in recent years. As an important energy source for cells, the health of mitochondria directly affects the vitality and lifespan of cells. Therefore, protecting mitochondria and maintaining cell energy have become new focuses in the anti-aging field. It is foreseeable that more anti-aging skin care products and raw material products based on mitochondria will emerge in the market in the future.

[0003] The anti-aging research of lemon balm extract can be found in the following literature:

[0004] The application number is CN118453476A, and the subject is a patent application for a repair and anti-aging composition and a repair and anti-aging cosmetic;

[0005] The application number is CN119258200A, and the subject is a patent application for an anti-aging composition for increasing dermal collagen and a preparation method thereof;

[0006] The anti-aging research on epigallocatechin galloside or epigallocatechin gallate can be found in the following literature:

[0007] The application number is CN115887246A, and the subject is a patent application for a novel microcapsule composition with multiple anti-aging effects and its preparation method and application;

[0008] The applicant believes that mitochondrial dysfunction may cause increased oxidative stress and produce excessive reactive oxygen free radicals (ROS). ROS will further damage various components in the cell, including the mitochondria themselves, forming a vicious cycle, which will greatly reduce the energy produced by the mitochondria. Insufficient energy supply will lead to a decrease in the cell metabolic rate, affecting the normal physiological function of the cell, and then affecting the function of tissues and organs, and ultimately accelerating skin aging. Therefore, by removing ROS, mitochondria can be prevented from being damaged by oxidation, mitochondrial dysfunction can be improved, and mitochondrial energy levels can be increased, thereby improving skin aging. The antioxidant (DPPH free radical scavenging) ability of the above substances was verified to evaluate their ability to scavenge free radicals and improve mitochondrial dysfunction.

[0009] During the verification process, we found that the above substances have certain free radical scavenging capabilities, but their ability to improve mitochondrial dysfunction needs to be further improved. Summary of the invention

[0010] The object of the present invention is to provide a mitochondrial energy improvement composition based on plant stem cells. This composition combines sunflower bud extract, lemon balm extract, and EGCG (epigallocatechin gallate glucoside or epigallocatechin gallic acid ester). Compared with the free radical scavenging ability when each of the three is used alone, it can effectively improve the free radical scavenging ability of the composition. Further verification reveals that after the combination of the three, it has an obvious ability to improve mitochondrial dysfunction, enhance mitochondrial energy, and increase the content of type I collagen, which can be used as a potential alternative as an anti-wrinkle active ingredient in cosmetics.

[0011] Meanwhile, the present invention also provides the application and cosmetics based on this composition.

[0012] The specific solution of the present invention is as follows:

[0013] A mitochondrial energy improvement composition based on plant stem cells, comprising the following components in parts by weight:

[0014] Sunflower bud extract 0.0005 - 1 part;

[0015] Lemon balm extract 0.0005 - 1 part;

[0016] Epigallocatechin gallate glucoside or epigallocatechin gallic acid ester 0.0005 - 1 part.

[0017] In some embodiments of the present invention, the dosage of the sunflower bud extract can be selected as 0.0005 part, 0.001 part, 0.005 part, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part;

[0018] The dosage of the lemon balm extract can be selected as 0.0005 part, 0.001 part, 0.005 part, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part;

[0019] The dosage of the epigallocatechin gallate glucoside or epigallocatechin gallic acid ester can be selected as 0.0005 part, 0.001 part, 0.005 part, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part;

[0020] In the present invention, the lemon balm extract is a plant stem cell extract. When the lemon balm extract is used alone, its free radical scavenging rate is very low; when EGCG is used alone, its free radical scavenging rate is significantly better than that of either the sunflower bud extract or the lemon balm extract. When EGCG is combined with either the sunflower bud extract or the lemon balm extract, certain synergism can be shown, and among them, the synergism between EGCG and the lemon balm extract is more obvious.

[0021] In subsequent experiments of the present invention, it was found that when sunflower sprout extract and lemon balm extract are used in combination in the presence of EGCG, their synergistic effect can be further improved.

[0022] When performing mitochondrial membrane potential tests, we further demonstrated a potential direct correlation between good free radical scavenging rate and mitochondrial membrane potential.

[0023] We believe that the underlying mechanism is as follows:

[0024] Mitochondrial dysfunction generally refers to the energy metabolism disorder caused by factors such as damaged mitochondrial membranes, blocked respiratory chains, decreased enzyme activity, and mitochondrial DNA (mtDNA) damage, which will trigger a series of chain reactions and cause cell damage. Further research reveals that oxidative stress is the key factor leading to mitochondrial dysfunction.

[0025] Sunflower sprout extract improves the function of the mitochondrial respiratory chain by increasing the level of NAD+, while lemon balm extract maintains the morphological integrity of mitochondria by protecting them from reactive oxygen species (ROS). As the core active ingredient in green tea, EGCG is known as the "diamond molecule in green tea" and has extremely strong antioxidant ability, which is 25 times that of vitamin C and 3 times that of pomegranate seed extract. The strong antioxidant effect of EGCG can further protect mitochondria from oxidative damage by free radicals. From the results, it is speculated that under the action of EGCG, the health status of mitochondria will be significantly improved, showing a significant synergistic effect. From the characteristics of each raw material, the mechanism of improving mitochondrial energy of the composition is based on the powerful antioxidant activity of EGCG to resist the oxidative damage of mitochondria by free radicals, the cytohesin contained in lemon balm extract preserves the integrity of cell nutrients and its antioxidant ability can further ensure the health of mitochondria, and sunflower sprout extract enhances the skin's production of NAD+ to increase the ATP level, improve DNA repair function, optimize cell function, and increase cell vitality; through the multiple external damage resistance, maintaining the integrity of the cell itself, and DNA repair inside the cell by the above three active ingredients, the improvement of mitochondrial energy is achieved through multiple external and internal coordinated cooperation, and then skin wrinkles are improved.

[0026] At the same time, through the test of the expression of type I collagen of each substance, it can be found that sunflower sprout extract, lemon balm extract, and EGCG can all show a certain effect of increasing the expression of type I collagen, among which the effect of lemon balm extract is more obvious, and when the three are used in combination, the effect is significantly improved.

[0027] In the above composition, it also includes one or more combinations of extracts of Leontopodium alpinum callus, Polygonum bistorta root, and Saponaria officinalis extract.

[0028] The callus extract of Leontopodium alpinum, the root extract of Polygonum bistorta, and the extract of Saponaria officinalis are also plant stem cell extracts. They have been used in a moisturizing product of the applicant and achieved good results. During the research process of this application, it is hoped that they can show certain effects in the formula of the present invention. Based on the experimental results obtained, their free radical scavenging ability is limited and they have no negative impact on mitochondrial membrane potential. Through the expression test experiment of type I collagen, it can be seen that the callus extract of Leontopodium alpinum, the root extract of Polygonum bistorta, and the extract of Saponaria officinalis can improve the expression of type I collagen to a certain extent. When combined with the sunflower sprout extract, the Melissa officinalis extract, and EGCG, they can play a more obvious role in enhancing the expression of type I collagen;

[0029] This also indicates that skin anti-wrinkle is the result of the comprehensive action of multi-faceted and multi-channel factors. Although the effects of the callus extract of Leontopodium alpinum, the root extract of Polygonum bistorta, and the extract of Saponaria officinalis and the sunflower sprout extract, the Melissa officinalis extract, and EGCG on mitochondrial membrane potential are additive and there is no synergistic effect, they have obvious synergy in the expression of type I collagen.

[0030] In the above composition, the dosage of the callus extract of Leontopodium alpinum is 0 - 1 part by weight;

[0031] The dosage of the root extract of Polygonum bistorta is 0 - 1 part by weight;

[0032] The dosage of the extract of Saponaria officinalis is 0 - 1 part by weight.

[0033] In the above composition, the dosage of the callus extract of Leontopodium alpinum is 0.001 - 1 part by weight;

[0034] The dosage of the root extract of Polygonum bistorta is 0.001 - 1 part by weight;

[0035] The dosage of the extract of Saponaria officinalis is 0.001 - 1 part by weight.

[0036] Meanwhile, the present invention also discloses the use of the composition as described in any one of the above in the preparation of cosmetics.

[0037] In the above use, the cosmetics are cosmetics for enhancing the energy of cell mitochondria.

[0038] In addition, the present invention also discloses a cosmetic containing the composition as described in any one of the above.

[0039] In the above cosmetic, the composition is contained in an amount of 0.001 - 10 wt%.

[0040] In the above-mentioned cosmetics, the dosage forms of the cosmetics at least include lotion, essence, dew, emulsion, cream and facial mask.

[0041] The beneficial effects of this application are as follows:

[0042] The combination of the sunflower sprout extract, lemon balm extract, epigallocatechin gallate or epigallocatechin gallate in the composition of the present invention can effectively improve the free radical scavenging ability of the composition; further verification found that the combination of the three has an obvious ability to improve mitochondrial dysfunction and increase mitochondrial energy, thereby improving the anti-wrinkle ability of the skin. Detailed implementation manners

[0043] Next, the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that for those conditions not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For the reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained through commercial purchase.

[0044] The first part

[0045] Description of the source of reagents:

[0046] The sunflower sprout extract is purchased from Guangzhou Lingyu Trading Co., Ltd., and its content is 0.1-5 wt%.

[0047] The lemon balm extract is purchased from Guangzhou Aoxue Chemical Co., Ltd., and its content is 3-10 wt%.

[0048] The EGCG is purchased from Guangzhou Aoxue Chemical Co., Ltd., and its content is 5-10 wt%.

[0049] The extract of Leontopodium alpinum callus is purchased from Guangzhou Aoyuan Trading Co., Ltd., and its content is 15-30 wt%.

[0050] The extract of Polygonum bistorta root is purchased from Guangzhou Lingyu Trading Co., Ltd., and its content is 0.5-1 wt%.

[0051] The extract of Saponaria officinalis is purchased from Guangzhou Lingyu Trading Co., Ltd., and its content is 0.5-1 wt%.

[0052] There will be different manufacturers for actual procurement, and the content will fluctuate slightly.

[0053] The second part

[0054] Example 1

[0055] The preparation method of the composition of the present invention is as follows:

[0056] Dissolve sunflower sprout extract, lemon balm extract, EGCG, Leontopodium alpinum callus extract, polygonatum root extract, and saponaria extract in water and stir until completely dissolved.

[0057] The formulation of the composition refers to Table 1;

[0058] Table 1 Formulation Table Unit: g

[0059] Sunflower bud extract Melissa officinalis extract EGCG Leontopodium alpinum callus extract Polygonum bistorta root extract Saponaria officinalis extract Water Example 1 0.0005 0.0005 0.0005 0 0 0 To100 Example 2 1 1 1 0 0 0 To100 Example 3 0.0005 0.01 0.5 0 0 0 To100 Example 4 0.01 0.0005 0.8 0 0 0 To100 Example 5 0.5 0.5 0.0005 0 0 0 To100 Example 6 0.01 0.01 0.002 0 0 0 To100 Example 7 0.01 0.01 0.002 0.001 0.001 0.001 To100 Example 8 0.01 0.01 0.002 1 1 1 To100 Example 9 0.01 0.01 0.002 0.5 0.5 0.5 To100 Comparative Example 1 0 0 0 0.001 0.001 0.001 To100 Comparative Example 2 0 0 0 0.5 0.5 0.5 To100 Comparative Example 3 0.01 0.01 0 0 0 0 To100 Comparative Example 4 0.02 0 0.002 0 0 0 To100 Comparative Example 5 0 0.02 0.002 0 0 0 To100 Comparative Example 6 0.02 0 0 0 0 0 To100 Comparative Example 7 0 0.02 0 0 0 0 To100 Comparative Example 8 0 0 0.002 0 0 0 To100

[0060] Part Three

[0061] 3.1 DPPH Radical Scavenging Rate

[0062] Weigh 0.005 g of DPPH (1,1-diphenyl-2-picrylhydrazine) powder, dissolve it in an appropriate amount of absolute ethanol, and ultrasonicate it in the dark to fully dissolve. Then, make up the volume to 100 mL with absolute ethanol to prepare a 50.0 μg / mL DPPH solution. Use purified water to prepare the samples of each example and comparative example respectively. In the experimental group, 1 mL of the sample is mixed with 3 mL of the DPPH solution. In the control group, 1 mL of the sample is mixed with 3 mL of absolute ethanol. In the blank group, 1 mL of the sample solvent is mixed with 3 mL of the DPPH solution. After thorough mixing, incubate in the dark for 30 min. Add 200 μL of the reaction solution of each group to a 96-well plate, with 3 parallel wells for each tube, and measure the absorbance at 517 nm.

[0063] Calculate its radical scavenging rate according to the following formula.

[0064] Scavenging rate (%) = [1 - (A 实验组 - A 对照组 ) / A 空白组 × 100%;

[0065] A 实验组 —— Absorbance of the mixed solution of the sample solution to be measured and the DPPH solution;

[0066] A 对照组 —— Absorbance of the mixed solution of the sample solution to be measured and the absolute ethanol solution;

[0067] A 空白组 —— Absorbance of the mixed solution of the DPPH solution and the sample solvent solution.

[0068] The DPPH radical scavenging rate reflects its antioxidant ability to a certain extent. The higher the scavenging rate, the better its antioxidant degree and the better the anti-wrinkle effect. Therefore, the anti-wrinkle effect can be judged by studying the ability of the composition to scavenge DPPH radicals.

[0069] 3.2 Mitochondrial Membrane Potential

[0070] HaCaT cells were seeded in 12-well plates (5×10 4 cells / well), incubated at 37 °C and 5% CO2 for 24 h. The experiment was set up with a normal control group, a model control group, and a sample group. The medium was removed, and the cells were washed 1-2 times with D-Hanks. The sample group and the model control group were subjected to UVB modeling (100 mJ / cm 2 ). After modeling, the cells were washed 1-2 times with D-Hanks. The normal control group and the model control group were added with complete medium (the final content of each component is shown in Table 1), and the sample group was added with complete medium containing the samples of the corresponding concentrations of the examples and the comparative examples, and continued to be cultured at 37 °C and 5% CO2 for 24 h. After the culture was completed, JC-1 staining was performed, and the cells were observed and photographed under a fluorescence microscope. Image J was used to analyze the fluorescence intensity (S) of each group, and the mitochondrial membrane potential was represented by the ratio of red / green fluorescence intensity. The mitochondrial membrane potential (ratio of red / green fluorescence intensity) was calculated.

[0071] Mitochondrial membrane potential (ratio of red / green fluorescence intensity) = S (red fluorescence intensity) / S (green fluorescence intensity).

[0072] The samples used for the mitochondrial membrane potential were Example 6, Example 7, and Comparative Example 1.

[0073] The mitochondrial membrane potential elevation rate was calculated according to the following formula:

[0074] Mitochondrial membrane potential elevation rate = (mitochondrial membrane potential of the sample group - mitochondrial membrane potential of the model control group) / mitochondrial membrane potential of the model control group × 100%

[0075] 3.3 Content of type I collagen

[0076] ESF-M cells were seeded in a 24-well plate and incubated at 37°C, 5% CO2 for 24 h. The experiment set up a normal control group, a model control group and a sample group. The culture medium in the well was discarded, and the sample group was added with complete culture medium containing corresponding concentrations of the examples and comparative examples (the final content of each component is shown in Table 1), and continued to be cultured at 37°C, 5% CO2 for 24 h; the cell plate was removed, the supernatant was discarded, and the cells were washed with PBS, and the cells were exposed to UVA for irradiation; after the irradiation, the PBS was discarded, and each group was added with complete culture medium and placed in a cell culture incubator for continued culture. Remove the culture plate, discard the culture medium in the well, add PBS to wash and discard; Add paraformaldehyde fixative to fix the cells, add PBS to wash and discard; Add Triton X-100 solution to permeabilize, add PBS to wash and discard; Add bovine serum albumin solution to block, add PBS to wash and discard; Add Rabbit Anti-Collagen I antibody and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) for staining and incubation, add PBS to wash and discard; DAPI staining solution, add PBS to wash and discard, add appropriate amount of anti-fluorescence quencher; Observe and take pictures under a fluorescence microscope. Use ImageJ to count the fluorescence intensity of each group of green fluorescence images.

[0077] The increase rate of type I collagen content = (green fluorescence intensity of the sample group - green fluorescence intensity of the model control group) / green fluorescence intensity of the model control group × 100%.

[0078] The test results can be seen in Table 2;

[0079] Table 2 Test result statistics

[0080] DPPH radical scavenging rate % Mitochondrial membrane potential elevation rate % I - type collagen content elevation rate % Example 1 20.50 / / Example 2 91.38 / / Example 3 95.84 / / Example 4 95.79 / / Example 5 83.96 / / Example 6 50.43 532 235.14 Example 7 62.75 571 386.75 Example 8 81.85 / / Example 9 75.87 / / Comparative Example 1 5.36 98 81.65 Comparative Example 2 23.31 / / Comparative Example 3 6.77 / / Comparative Example 4 30.11 / / Comparative Example 5 40.15 / / Comparative Example 6 4.01 / 76.52 Comparative Example 7 7.82 / 57.88 Comparative Example 8 20.67 / 96.04

[0081] Result analysis:

[0082] 1. According to the test results of Example 1, Example 2, Example 3, Example 4, Example 5, and Example 6, the free radical scavenging ability is closely related to the concentration of the effective ingredient; from the experimental results of Example 3, Example 4, and Example 5, when the dosage of any one of the sunflower sprout extract, the lemon balm extract, and the EGCG is very small, the composition can show a relatively excellent free radical scavenging effect; among them, the effect of Example 5 is relatively weak, the reason is that among the three, EGCG has the strongest single antioxidant ability, and when its dosage is small, the dosage of the other two is required to increase in order to achieve a relatively good free radical scavenging effect; this conclusion can be supported by the data of the free radical scavenging ability of Comparative Example 8.

[0083] 2. The test results of Example 6 and Comparative Examples 3 to 5 can prove that if in the form of pairwise combinations, EGCG is the most important component for scavenging free radicals;

[0084] 3. Considering the data of Comparative Examples 6 to 8 and Comparative Examples 3 to 5, there is no obvious synergy between any two of the sunflower sprout extract, lemon balm extract, and EGCG; from the results of Example 6 and Comparative Examples 6 to 8, there is an obvious internal synergy among the above three;

[0085] From the test results of Comparative Example 3 and Comparative Example 8, Comparative Example 4 and Comparative Example 7, and Comparative Example 5 and Comparative Example 6, it can be seen that the sunflower sprout extract is the core for generating synergy. From the previous research of this application, it can be concluded that the sunflower sprout extract has the weakest free radical scavenging ability among all substances, and its ability to significantly enhance the antioxidant properties of the latter two exceeds the expectation of the pre-experimental design.

[0086] 4. Considering the results of Comparative Example 6, Comparative Example 7, Comparative Example 1, Example 9, and Comparative Example 2, the exhibition of the free radical scavenging ability of the Leontopodium alpinum callus extract, Polygonum bistorta root extract, and Saponaria officinalis extract requires a certain concentration. This result can be concluded from the data change trends of Examples 7 to 9 and Comparative Examples 1 to 2. It can be inferred that it may show the same trend in the mitochondrial membrane potential elevation rate. Based on the performance evaluation results of the products already on sale by this applicant, even if it does not show good free radical scavenging effects at low concentrations, it will effectively compensate for the composition of the present invention in terms of properties such as moisturizing.

[0087] 5. From the test results of the elevation rate of type I collagen content, compared with the use of a single component among the sunflower sprout extract, lemon balm extract, and EGCG, the expression of type I collagen in Example 6 has been significantly improved. At the same time, referring to the results of Example 6, Example 7, and Comparative Example 1, the Leontopodium alpinum callus extract, Polygonum bistorta root extract, and Saponaria officinalis extract can increase the expression of type I collagen to a certain extent. When combined with the sunflower sprout extract, lemon balm extract, and EGCG, it can play a more obvious role in increasing the content of type I collagen.

[0088] 3.3 Safety Test

[0089] Select qualified patch test materials. Put the test substances (Example 2, Example 8) into the patch tester, with a dosage of about 0.020 mL - 0.025 mL (the liquid is dropped on the filter paper attached to the patch tester and placed in the patch tester). Stick the patch tester with the test substance on the back or the flexor side of the forearm of the subject, and gently press it with the palm to make it evenly adhere to the skin for 24 hours.

[0090] The judgment criteria are shown in Table 3 (Skin Reaction Judgment Criteria for Closed Patch Test);

[0091] Table 3 Skin Reaction Judgment Criteria for Closed Patch Test

[0092] Reaction degree Scoring grade Skin reaction - 0 Negative reaction ± 1 Suspicious reaction: only slight erythema + 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, may have papules ++ 3 Strong positive reaction (vesicle reaction): erythema, infiltration, edema, papules, vesicles; the reaction may exceed the test area +++ 4 Extremely strong positive reaction (confluent vesicle reaction): obvious erythema, severe infiltration, edema, confluent vesicles; the reaction exceeds the test area

[0093] Result judgment: When the number of subjects with grade 1 skin adverse reactions among 30 subjects is more than 5, or the number of subjects with grade 2 skin adverse reactions is more than 2 (the number of subjects with grade 2 reactions in the patch test of the deodorant product is more than 5), or when any 1 case of grade 3 or above skin adverse reaction occurs, it is determined that the test substance has skin adverse reactions to the human body. The test results can be seen in Table 4.

[0094] Table 4 Test Results of Closed Patch Skin Reaction Experiment

[0095]

[0096] Conclusion: The results of the human patch test show that:

[0097] Among 30 subjects, the skin of [number of subjects] cases showed negative reactions (scoring grade 0). Referring to the judgment criteria of the human patch test in "HAT-SOP-F1-PZ-092", Example 2 and Example 8 have no adverse reactions to human skin.

Claims

1. A mitochondrial energy improvement composition based on plant stem cells, characterized in that, It consists of the following components in parts by weight: Sunflower sprout extract: 0.01 - 0.5 part; Melissa officinalis extract: 0.01 - 0.5 part; Epigallocatechin gallate glucoside: 0.002 - 0.5 part; Leontopodium alpinum callus extract: 0 - 1 part by weight; Symphytum officinale root extract: 0 - 1 part by weight; Saponaria officinalis extract: 0 - 1 part by weight; The content of the active ingredient in the sunflower sprout extract is 0.1 - 5 wt%; The content of the active ingredient in the Melissa officinalis extract is 3 - 10 wt%; The content of the active ingredient in the epigallocatechin gallate glucoside is 5 - 10 wt%; The content of the active ingredient in the Leontopodium alpinum callus extract is 15 - 30 wt%; The content of the active ingredient in the Symphytum officinale root extract is 0.5 - 1 wt%; The content of the active ingredient in the Saponaria officinalis extract is 0.5 - 1 wt%.

2. The composition according to claim 1, wherein The dosage of the Leontopodium alpinum callus extract is 0.001 - 1 part by weight; The dosage of the Symphytum officinale root extract is 0.001 - 1 part by weight; The dosage of the Saponaria officinalis extract is 0.001 - 1 part by weight.

3. Use of the composition according to claim 1 or 2 for preparing cosmetics.

4. The use according to claim 3, characterized in that, The cosmetics are cosmetics for enhancing the energy of cell mitochondria.

5. A cosmetic, characterized in that, Containing the composition according to claim 1 or 2.

6. The cosmetic according to claim 5, characterized in that, Containing 0.001 - 10 wt% of the composition according to claim 1 or 2.

7. The cosmetic according to claim 6, characterized in that, The dosage form of the cosmetics is lotion, essence, emulsion, cream or facial mask.

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